BR112012015443A2 - métodos de detecção de micro-organismos e kits para os mesmos - Google Patents

métodos de detecção de micro-organismos e kits para os mesmos

Info

Publication number
BR112012015443A2
BR112012015443A2 BR112012015443A BR112012015443A BR112012015443A2 BR 112012015443 A2 BR112012015443 A2 BR 112012015443A2 BR 112012015443 A BR112012015443 A BR 112012015443A BR 112012015443 A BR112012015443 A BR 112012015443A BR 112012015443 A2 BR112012015443 A2 BR 112012015443A2
Authority
BR
Brazil
Prior art keywords
fluorescently
syn
microorganism detection
detectable product
test sample
Prior art date
Application number
BR112012015443A
Other languages
English (en)
Other versions
BR112012015443B1 (pt
Inventor
Phillip A Bolea
Stephanie J Moeller
Stephen B Roscoe
Original Assignee
3M Innovative Properties Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 3M Innovative Properties Co filed Critical 3M Innovative Properties Co
Publication of BR112012015443A2 publication Critical patent/BR112012015443A2/pt
Publication of BR112012015443B1 publication Critical patent/BR112012015443B1/pt

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

métodos de detecção de micro-organismo e kits para os mesmos a presente invenção refere-se a um método de detecção de micro-organimos em uma amostra de teste. o método inclui as etapas de : a) incubar a amostra de teste com um meio de crescimento para formar uma amostra incubada , sendo que o meio de crescimento inclui um substrato de enzima e o substrato de enzima inclui um grupo enzimaticamente hidrolisável e um grupo fluorescente ,sendo que os micro -organismos presentes na amostra de teste incluem uma enzima que hidrolisa o grupo hidrolísavel do grupo fluorescente para formar um produto detectável de forma fluorescente ,sendo que o produto detectável de formar fluorescente tem espéciaes ácidas e básicas ; b) excitar o produto detectável de forma fluorescente com luz que tem comprimento de onda ex<syn>iso durante tempo suficiente para que o produto detectável de forma fluorescente emita luz , sendo que ex<syn>iso é ponto isosbéstico de abordância do produto detectável de forma fluorescente ; e c) detectar luz emitida em um comprimento de onda em<syn>1.
BR112012015443-5A 2009-12-22 2010-12-17 Método de detecção de micro-organismos em uma amostra de teste BR112012015443B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28888309P 2009-12-22 2009-12-22
US61/288,883 2009-12-22
PCT/US2010/060936 WO2011087711A1 (en) 2009-12-22 2010-12-17 Methods of detecting microorganisms and kits therefore

Publications (2)

Publication Number Publication Date
BR112012015443A2 true BR112012015443A2 (pt) 2016-03-15
BR112012015443B1 BR112012015443B1 (pt) 2019-07-02

Family

ID=43855961

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012015443-5A BR112012015443B1 (pt) 2009-12-22 2010-12-17 Método de detecção de micro-organismos em uma amostra de teste

Country Status (6)

Country Link
US (2) US8518664B2 (pt)
EP (1) EP2516666B1 (pt)
JP (1) JP5747043B2 (pt)
CN (1) CN102686739B (pt)
BR (1) BR112012015443B1 (pt)
WO (1) WO2011087711A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102686739B (zh) * 2009-12-22 2014-09-24 3M创新有限公司 检测微生物的方法和其套件
CN103343158B (zh) * 2013-07-20 2014-05-14 吉林农业大学 产气荚膜梭菌检测纸片、制备方法及其应用
US10379125B2 (en) 2013-12-27 2019-08-13 Becton, Dickinson And Company System and method for dynamically calibrating and measuring analyte concentration in diabetes management monitors
CN106163996B (zh) * 2014-04-09 2019-09-10 Nch公司 用于检测在水系统中的微生物生长的系统和方法
US20180237821A1 (en) * 2017-02-23 2018-08-23 Ethicon, Inc. Apparatus and method to read biological indicator
FR3103900A1 (fr) * 2019-11-29 2021-06-04 Universite Du Mans Méthode d'identification rapide de microorganismes par analyse de matrices excitation-émission
CN111560408B (zh) * 2020-02-29 2022-11-25 浙江工业大学 一种基于流动化学酶促在线合成香豆素-3-羧酸糖酯衍生物的方法
CN112679569B (zh) * 2020-10-22 2022-05-27 山西大学 一种荧光探针及其制备方法和应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5817598B2 (ja) 1979-10-31 1983-04-08 味の素株式会社 微生物の迅速検出方法
GB2128204B (en) * 1982-04-14 1987-02-25 Unilever Plc Microbiological test processes and apparatus
US5079144A (en) * 1982-04-14 1992-01-07 Radiometer Corporate Development Ltd. Microorganism testing with a hydrolyzable fluorogenic substrate
US5354655A (en) * 1988-03-29 1994-10-11 Biocontrol Systems, Inc. Method for determining the presence or concentration of a bound enzyme
US6387650B1 (en) 1995-06-07 2002-05-14 Biocontrol Systems, Inc. Method and composition for detecting bacterial contamination in food products
US6372895B1 (en) 2000-07-07 2002-04-16 3M Innovative Properties Company Fluorogenic compounds
US8663909B2 (en) * 2002-05-09 2014-03-04 Nanologix, Inc. Device for rapid detection and identification of single microorganisms without preliminary growth
US7402426B2 (en) 2002-09-20 2008-07-22 Queen's University At Kingston Detection of biological molecules by differential partitioning of enzyme substrates and products
EP1926825A4 (en) * 2005-09-20 2008-09-24 Univ Arizona State METHODS FOR DETECTING AND CHARACTERIZING MICROORGANISMS
CN102686739B (zh) * 2009-12-22 2014-09-24 3M创新有限公司 检测微生物的方法和其套件

Also Published As

Publication number Publication date
CN102686739A (zh) 2012-09-19
US20130316443A1 (en) 2013-11-28
US8518664B2 (en) 2013-08-27
WO2011087711A1 (en) 2011-07-21
CN102686739B (zh) 2014-09-24
BR112012015443B1 (pt) 2019-07-02
EP2516666B1 (en) 2016-04-20
JP2013514809A (ja) 2013-05-02
US20120252049A1 (en) 2012-10-04
US8802429B2 (en) 2014-08-12
JP5747043B2 (ja) 2015-07-08
EP2516666A1 (en) 2012-10-31

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Legal Events

Date Code Title Description
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 17/12/2010, OBSERVADAS AS CONDICOES LEGAIS. (CO) 20 (VINTE) ANOS CONTADOS A PARTIR DE 17/12/2010, OBSERVADAS AS CONDICOES LEGAIS

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

Free format text: REFERENTE A 12A ANUIDADE.

B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2703 DE 25-10-2022 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.