CN102630821A - Aquacultural microbiological feed premix and preparation method thereof - Google Patents
Aquacultural microbiological feed premix and preparation method thereof Download PDFInfo
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- CN102630821A CN102630821A CN2012101006326A CN201210100632A CN102630821A CN 102630821 A CN102630821 A CN 102630821A CN 2012101006326 A CN2012101006326 A CN 2012101006326A CN 201210100632 A CN201210100632 A CN 201210100632A CN 102630821 A CN102630821 A CN 102630821A
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- 230000002906 microbiologic effect Effects 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 44
- 239000000463 material Substances 0.000 claims abstract description 32
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 29
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 29
- 108010059892 Cellulase Proteins 0.000 claims abstract description 29
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 29
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 29
- 229940106157 cellulase Drugs 0.000 claims abstract description 29
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 28
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 23
- 235000009566 rice Nutrition 0.000 claims abstract description 23
- 239000011780 sodium chloride Substances 0.000 claims abstract description 21
- 238000000855 fermentation Methods 0.000 claims abstract description 15
- 230000004151 fermentation Effects 0.000 claims abstract description 15
- 239000007787 solid Substances 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims description 57
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 56
- 239000001963 growth medium Substances 0.000 claims description 50
- 238000002156 mixing Methods 0.000 claims description 38
- 241000894006 Bacteria Species 0.000 claims description 34
- 239000003795 chemical substances by application Substances 0.000 claims description 32
- 108010089934 carbohydrase Proteins 0.000 claims description 28
- 235000014655 lactic acid Nutrition 0.000 claims description 28
- 239000004310 lactic acid Substances 0.000 claims description 28
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 23
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 23
- 241000209094 Oryza Species 0.000 claims description 22
- 235000002639 sodium chloride Nutrition 0.000 claims description 20
- 238000009360 aquaculture Methods 0.000 claims description 19
- 244000144974 aquaculture Species 0.000 claims description 19
- 239000000203 mixture Substances 0.000 claims description 8
- 238000007865 diluting Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 abstract description 16
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 230000035764 nutrition Effects 0.000 abstract description 3
- 238000012258 culturing Methods 0.000 abstract description 2
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 abstract 1
- 102100022624 Glucoamylase Human genes 0.000 abstract 1
- 241000186660 Lactobacillus Species 0.000 abstract 1
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 235000019764 Soybean Meal Nutrition 0.000 abstract 1
- 230000036039 immunity Effects 0.000 abstract 1
- 229940039696 lactobacillus Drugs 0.000 abstract 1
- 239000004455 soybean meal Substances 0.000 abstract 1
- 244000005700 microbiome Species 0.000 description 10
- 108090000790 Enzymes Proteins 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- 229940088598 enzyme Drugs 0.000 description 6
- 230000009286 beneficial effect Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 241000233866 Fungi Species 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 230000002180 anti-stress Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000003053 immunization Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000019568 aromas Nutrition 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000005714 functional activity Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000003871 intestinal function Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000007269 microbial metabolism Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001835 salubrious effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
Abstract
The invention discloses aquacultural microbiological feed premix and a preparation method thereof. The aquacultural microbiological feed premix comprises culture material and medium. The culture material comprises, in weight percentage, 25-32% of lactobacillus, 28-35% of bacillus subtilis, 8-12% of brewer's yeast, 17-20% of glucoamylase and 8-13% of cellulase. The medium comprises, in weight percentage, 30-35% of rice bran, 15-20% of soybean meal, 0.8-1% of NaCl and 44-54.2% of wheat bran. The culture material and the medium are mixed for solid fermentation so as to obtain the microbiological feed premix. By the aquacultural microbiological feed premix, animal immunity is improved, animal body color is improved, the rate of absorbing feed nutrition is increased, and culturing cycle is shortened.
Description
Technical field
The present invention relates to aquaculture field, relate in particular to a kind of aquaculture microbiological feed pre-mixing agent and preparation method thereof.Belong to microbiological feed and preparing technical field.
Background technology
Along with the fast development of aquaculture, feedstuff prices such as fish meal, dregs of beans are unprecedented soaring, and feed cost is high, has seriously restricted the lifting of aquaculture benefit.Meanwhile, because naturally occurring all kinds of ANFs (trypsin ihhibitor, urase, soybean haemoglutinin etc.), amino acid composition imbalance have influenced the animal digestion absorbing function in the feed ingredient, thereby cause the efficiency of feed utilization reduction.Along with development of biology, the particularly development of microbial technique, people gradually adopt the microbiological feed fermentation technique to improve trophic structure, lifting animal digestion absorbability in the feed.Realize with enzyme preparation through in feed, adding beneficial bacteria of intestinal tract generally speaking, make beneficial microbe field planting and form advantage group in animal alimentary canal on the one hand, promote the microecological balance in the alimentary canal, the ability of digesting and assimilating of raising animal; On the other hand, can produce functional activity materials such as little peptide, glucan in the microbial metabolism, the toxin that possibly exist in the degraded feedstuff promotes the animal immune function, ensures animal health; Meanwhile, complex enzyme formulation can efficiently decompose feedstuff, helps it and is digested and assimilated.
But, be used for the microbiological feed pre-mixing agent product of aquaculture and the problem that there is following aspect in technology thereof in the market: the one, microorganism and enzyme proportion of composing are unreasonable, and the synergy of microorganism and enzyme is not obvious; The 2nd, living bacteria count is on the low side, and service efficiency is not high; Usually adopt liquid fermentation process, pollution risk is bigger in storage, the transportation.
Summary of the invention
One of the object of the invention is for a kind of aquaculture microbiological feed pre-mixing agent is provided.Can improve animal immunizing power, improve the animal body colour, strengthen the animal anti-stress ability, and improve the absorptivity of feed nutrition, reduce feed coefficient, thereby shorten the culture-cycle.
Two of the object of the invention is for a kind of preparation method of aquaculture microbiological feed pre-mixing agent is provided.
One of the object of the invention can reach through adopting following technical scheme:
A kind of aquaculture microbiological feed pre-mixing agent; Comprise strain raw material and culture medium; Said strain raw material is made up of lactic acid bacteria, bacillus subtilis, saccharomyces cerevisiae, carbohydrase and cellulase, and to account for the strain raw material weight percentage be lactic acid bacteria 25～32%, bacillus subtilis 28～35%, saccharomyces cerevisiae 8～12%, carbohydrase 17～20% and cellulase 8～13% to each material component in the strain raw material; Said culture medium is made up of rice bran, dregs of beans, NaCl and wheat bran, and to account for the weight percentage of culture medium be rice bran 30～35%, dregs of beans 15～20%, NaCl 0.8～1% and wheat bran 44～54.2% to each material component in the culture medium; Obtain the microbiological feed pre-mixing agent by carrying out solid fermentation after strain raw material and the culture medium mixing.
One of the object of the invention can also reach through taking following technical scheme:
A kind of Technological improvement plan of realizing one of the object of the invention is: to account for the strain raw material weight percentage be lactic acid bacteria 28%, bacillus subtilis 30%, saccharomyces cerevisiae 9%, carbohydrase 20% and cellulase 13% to each material component in the strain raw material; To account for the weight percentage of culture medium be rice bran 30%, dregs of beans 17%, NaCl 0.8% and wheat bran 52.2% to each material component in the culture medium.
A kind of Technological improvement plan of realizing one of the object of the invention is:
The viable count of said lactic acid bacteria is 2～2.5 hundred million/and the viable count of gram, bacillus subtilis is that the viable count of 5～600,000,000/gram, saccharomyces cerevisiae is 3～3.5 hundred million/gram, and said carbohydrase content is that 50～60IU/ gram, cellulase content are 10～12IU/ gram; Said wheat bran is 60～80 mesh sieve.
Two of the object of the invention can reach through taking following technical scheme:
A kind of preparation method of aquaculture microbiological feed pre-mixing agent,
1) is ready to strain raw material; Said strain raw material is made up of lactic acid bacteria, bacillus subtilis, saccharomyces cerevisiae, carbohydrase and cellulase, and to account for the strain raw material weight percentage be lactic acid bacteria 25～32%, bacillus subtilis 28～35%, saccharomyces cerevisiae 8～12%, carbohydrase 17～20% and cellulase 8～13% to each material component in the strain raw material;
2) be ready to culture medium, said culture medium is made up of rice bran, dregs of beans, NaCl and wheat bran, and to account for the weight percentage of culture medium be rice bran 30～35%, dregs of beans 15～20%, NaCl 0.8～1% and wheat bran 44～54.2% to each material component in the culture medium;
3) ready strain raw material is diluted, earlier with the lactic acid bacteria in the strain raw material, bacillus subtilis, saccharomyces cerevisiae, carbohydrase and cellulase mixing, the water by 40 kilograms of 20～25 gram strain raw materials addings carries out diluted mixture again.
4) carry out solid fermentation; It is even at first fermentation auxiliary material to be that rice bran in the culture medium, dregs of beans, NaCl and wheat bran mix the companion in proportion; And then the strain raw material after will diluting mixes stirring with culture medium by 1: 1.5 weight ratio; And then sealing, place it in the dark fermented and cultured 72h of place at last, obtain the microbiological feed pre-mixing agent.
Beneficial effect of the present invention:
1, the present invention is owing to taked the prescription through the specific proportioning of applicant's repetition test and checking, and the proportion of composing of rational allocation microorganism fungus kind and enzyme makes the synergy of microorganism and enzyme obvious; Effectively improve viable count, improved service efficiency.
2, the present invention adopts solid fermentation process, has storage, convenient transportation, prevents to pollute and protect the beneficial effect of environment.
3, the present invention is owing to be provided with specific material prescription and under specific process conditions, carry out fermenting and producing, and therefore, the microbiological feed of preparation has good product quality, production cost is low and production efficiency is high beneficial effect.
The practical implementation method
Below in conjunction with embodiment the present invention is done further explanation.
Embodiment 1:
The aquaculture microbiological feed pre-mixing agent that present embodiment relates to; Comprise microorganism fungus kind raw material and culture medium; Said strain raw material is made up of lactic acid bacteria, bacillus subtilis, saccharomyces cerevisiae, carbohydrase and cellulase, and to account for the strain raw material weight percentage be lactic acid bacteria 28%, bacillus subtilis 30%, saccharomyces cerevisiae 9%, carbohydrase 20% and cellulase 13% to each material component in the strain raw material; To account for the weight percentage of culture medium be rice bran 30%, dregs of beans 17%, NaCl 0.8% and wheat bran 52.2% to each material component in the culture medium, carries out solid fermentation after being mixed by strain raw material and culture medium and obtain the microbiological feed pre-mixing agent.
In the present embodiment, the viable count of said lactic acid bacteria is 2～2.5 hundred million/viable count of gram, bacillus subtilis is that the viable count of 5～600,000,000/gram, saccharomyces cerevisiae is that 3～3.5 hundred million/gram, carbohydrase content are that 50～60IU/ gram and cellulase content are 10～12IU/ gram; Said wheat bran is 60～80 mesh sieve.
The preparation method of present embodiment comprises the steps:
1) is ready to strain raw material; Said strain raw material is made up of lactic acid bacteria, bacillus subtilis, saccharomyces cerevisiae, carbohydrase and cellulase, and to account for the strain raw material weight percentage be lactic acid bacteria 28%, bacillus subtilis 30%, saccharomyces cerevisiae 9%, carbohydrase 20% and cellulase 13% to each material component in the strain raw material;
2) be ready to culture medium, said culture medium is made up of rice bran, dregs of beans, NaCl and wheat bran, and to account for the weight percentage of culture medium be rice bran 30%, dregs of beans 17%, NaCl 0.8% and wheat bran 52.2% to each material component in the culture medium.
3) ready strain raw material is diluted, earlier with the lactic acid bacteria in the strain raw material, bacillus subtilis, saccharomyces cerevisiae, carbohydrase and cellulase mixing, the water by 40 kilograms of 20 gram strain raw materials addings carries out diluted mixture again.
4) carry out solid fermentation; It is even at first fermentation auxiliary material to be that rice bran in the culture medium, dregs of beans, NaCl and wheat bran mix the companion in proportion; And then 20 after will diluting gram strain raw material and 60 kilograms culture medium mixing stirring; And then the back envelope, place it in the dark fermented and cultured 72h of place at last, obtain the microbiological feed pre-mixing agent.
Thick protein (in dry) content in the microorganism pre-mixing agent that obtains at last reaches 10.3～11.9%, and living bacteria count is 1.17～1.24 hundred million a/gram.
The application process of present embodiment is following:
When brew, also carry out the proportioning of different proportion according to the different breed phases.Culturing early stage, the ratio of adding 20～30 kilograms of microbiological feed pre-mixing agents in 80 kilograms of feeds substitutes original 100 kilograms of feeds use; Culture mid-term, the ratio of adding 10～15 kilograms of microbiological feed pre-mixing agents in 80 kilograms of feeds substitutes original 100 kilograms of feeds use; Culture the later stage, the ratio of adding 5～10 kilograms of microbiological feed pre-mixing agents in 80 kilograms of feeds substitutes original 100 kilograms of feeds use.
Show through applicant's application, adopt the present invention to have following effect:
1. have fermenting aromas such as sweet, sour, alcohol, mouthfeel is good, has obviously improved animal feed intake;
2. thick protein is abundant;
3. effectively improved the animal intestinal micro-ecological environment, the rear intestinal function of searching for food is obviously improved, and reduces excreta, has significantly improved the feed nutrition absorptivity, has reduced feed coefficient, practices thrift feed, has shortened the culture-cycle;
4. improve animal immunizing power, improved the animal body colour, strengthened the animal anti-stress ability;
5. improved breeding environment, kept good bacterium phase, algae phase, made the long-term maintenance of environment salubrious.
Embodiment 2:
A kind of microbiological feed pre-mixing agent; Comprise microorganism fungus kind raw material and culture medium; Said strain raw material is made up of lactic acid bacteria, bacillus subtilis, saccharomyces cerevisiae, carbohydrase and cellulase, and to account for the strain raw material weight percentage be lactic acid bacteria 30%, bacillus subtilis 30%, saccharomyces cerevisiae 10%, carbohydrase 20% and cellulase 10% to each material component in the strain raw material; Said culture medium is made up of rice bran, dregs of beans, NaCl and wheat bran; To account for the weight percentage of culture medium be rice bran 32%, dregs of beans 18%, NaCl 0.9% and wheat bran 49.1% to each material component in the culture medium, carries out solid fermentation after being mixed by strain raw material and culture medium and obtain the microbiological feed pre-mixing agent.
The viable count of said lactic acid bacteria is 2～2.5 hundred million/viable count of gram, bacillus subtilis is that the viable count of 5～600,000,000/gram, saccharomyces cerevisiae is that 3～3.5 hundred million/gram, carbohydrase content are that 50～60IU/ gram and cellulase content are 10～12IU/ gram; Said wheat bran is 60～80 mesh sieve.
Its preparation method and step are with specific embodiment 1.
Thick protein (in dry) content in the microorganism pre-mixing agent that obtains at last reaches 10.3～11.9%, and living bacteria count is 1.17～1.24 hundred million a/gram.
Embodiment 3:
A kind of microbiological feed pre-mixing agent; Comprise microorganism fungus kind raw material and culture medium; Said strain raw material is made up of lactic acid bacteria, bacillus subtilis, saccharomyces cerevisiae, carbohydrase and cellulase, and to account for the strain raw material weight percentage be lactic acid bacteria 32%, bacillus subtilis 35%, saccharomyces cerevisiae 8%, carbohydrase 17% and cellulase 8% to each material component in the strain raw material; Said culture medium is made up of rice bran, dregs of beans, NaCl and wheat bran; To account for the weight percentage of culture medium be rice bran 35%, dregs of beans 20%, NaCl 1% and wheat bran 44% to each material component in the culture medium, carries out solid fermentation after being mixed by strain raw material and culture medium and obtain the microbiological feed pre-mixing agent.
The viable count of said lactic acid bacteria is 2～2.5 hundred million/viable count of gram, bacillus subtilis is that the viable count of 5～600,000,000/gram, saccharomyces cerevisiae is that 3～3.5 hundred million/gram, carbohydrase content are that 50～60IU/ gram and cellulase content are 10～12IU/ gram; Said wheat bran is 60～80 mesh sieve.
Its preparation method and step are with specific embodiment 1.
Thick protein (in dry) content in the microorganism pre-mixing agent that obtains at last reaches 10.3～11.9%, and living bacteria count is 1.17～1.24 hundred million a/gram.
Claims (8)
1. aquaculture microbiological feed pre-mixing agent; Comprise strain raw material and culture medium; It is characterized in that: said strain raw material is made up of lactic acid bacteria, bacillus subtilis, saccharomyces cerevisiae, carbohydrase and cellulase, and to account for the strain raw material weight percentage be lactic acid bacteria 25～32%, bacillus subtilis 28～35%, saccharomyces cerevisiae 8～12%, carbohydrase 17～20% and cellulase 8～13% to each material component in the strain raw material; Said culture medium is made up of rice bran, dregs of beans, NaCl and wheat bran, and to account for the weight percentage of culture medium be rice bran 30～35%, dregs of beans 15～20%, NaCl 0.8～1% and wheat bran 44～54.2% to each material component in the culture medium; Obtain the microbiological feed pre-mixing agent by carrying out solid fermentation after strain raw material and the culture medium mixing.
2. a kind of aquaculture microbiological feed pre-mixing agent according to claim 1 is characterized in that: and the viable count of said lactic acid bacteria is 2～2.5 hundred million/and the viable count of gram, bacillus subtilis is that the viable count of 5～600,000,000/gram, saccharomyces cerevisiae is 3～3.5 hundred million/gram.
3. a kind of aquaculture microbiological feed pre-mixing agent according to claim 1 is characterized in that: said carbohydrase content is that 50～60IU/ gram, cellulase content are 10～12IU/ gram.
4. a kind of aquaculture microbiological feed pre-mixing agent according to claim 1 is characterized in that: said wheat bran fineness degree is 60～80 order.
5. according to the described a kind of aquaculture microbiological feed pre-mixing agent of the arbitrary claim of claim 1 to 4, it is characterized in that: to account for the strain raw material weight percentage be lactic acid bacteria 28%, bacillus subtilis 30%, saccharomyces cerevisiae 9%, carbohydrase 20% and cellulase 13% to each material component in the strain raw material; To account for the weight percentage of culture medium be rice bran 30%, dregs of beans 17%, NaCl 0.8% and wheat bran 52.2% to each material component in the culture medium.
6. according to the described a kind of aquaculture microbiological feed pre-mixing agent of the arbitrary claim of claim 1 to 4, it is characterized in that: to account for the strain raw material weight percentage be lactic acid bacteria 30%, bacillus subtilis 30%, saccharomyces cerevisiae 10%, carbohydrase 20% and cellulase 10% to each material component in the strain raw material; To account for the weight percentage of culture medium be rice bran 32%, dregs of beans 18%, NaCl 0.9% and wheat bran 49.1% to each material component in the culture medium.
7. according to the described a kind of aquaculture microbiological feed pre-mixing agent of the arbitrary claim of claim 1 to 4, it is characterized in that: to account for the strain raw material weight percentage be lactic acid bacteria 32%, bacillus subtilis 35%, saccharomyces cerevisiae 8%, carbohydrase 17% and cellulase 8% to each material component in the strain raw material; To account for the weight percentage of culture medium be rice bran 35%, dregs of beans 20%, NaCl 1% and wheat bran 44% to each material component in the culture medium.
8. the preparation method of a kind of aquaculture microbiological feed pre-mixing agent according to claim 1 is characterized in that:
1) is ready to strain raw material; Said strain raw material is made up of lactic acid bacteria, bacillus subtilis, saccharomyces cerevisiae, carbohydrase and cellulase, and to account for the strain raw material weight percentage be lactic acid bacteria 25～32%, bacillus subtilis 28～35%, saccharomyces cerevisiae 8～12%, carbohydrase 17～20% and cellulase 8～13% to each material component in the strain raw material;
2) be ready to culture medium, said culture medium is made up of rice bran, dregs of beans, NaCl and wheat bran, and to account for the weight percentage of culture medium be rice bran 30～35%, dregs of beans 15～20%, NaCl 0.8～1% and wheat bran 44～54.2% to each material component in the culture medium;
3) ready strain raw material is diluted, earlier with the lactic acid bacteria in the strain raw material, bacillus subtilis, saccharomyces cerevisiae, carbohydrase and cellulase mixing, the water by 40 kilograms of 20～25 gram strain raw materials addings carries out diluted mixture again.
4) carry out solid fermentation; It is even at first fermentation auxiliary material to be that rice bran in the culture medium, dregs of beans, NaCl and wheat bran mix the companion in proportion; And then the strain raw material after will diluting mixes stirring with culture medium by 1: 1.5 weight ratio; And then sealing, place it in the dark fermented and cultured 72h of place at last, obtain the microbiological feed pre-mixing agent.
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| CN103283961A (en) * | 2013-05-10 | 2013-09-11 | 浙江工业大学 | Method for preparing health-benefiting fermented soybean meal |
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| CN106745762A (en) * | 2016-11-28 | 2017-05-31 | 中国水产科学研究院珠江水产研究所 | A kind of region breeding water body miniature organism sealing compound and preparation method thereof |
| CN108936098A (en) * | 2018-09-14 | 2018-12-07 | 安徽省农业科学院水产研究所 | A kind of production method of the dedicated ecological fermentation feed of Chinese snake head |
| CN110150461A (en) * | 2019-07-04 | 2019-08-23 | 徐州工程学院 | A kind of Composite fermentation type microbiological feed and preparation method thereof |
| CN111011665A (en) * | 2019-12-31 | 2020-04-17 | 山西大禹生物工程股份有限公司 | Microbial fermentation wet material for promoting growth of aquatic animals and preparation method and application thereof |
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| CN111772058A (en) * | 2020-08-04 | 2020-10-16 | 清远海贝生物技术有限公司 | Active compound feed suitable for aquaculture and preparation method and application thereof |
| CN112456653A (en) * | 2020-10-27 | 2021-03-09 | 广西诺亚生物科技发展有限公司 | Compound for adjusting aquaculture water quality and preparation process thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101978850A (en) * | 2010-12-04 | 2011-02-23 | 徐贵阁 | Green biological active feed |
| CN101986862A (en) * | 2009-07-31 | 2011-03-23 | 丁之铨 | Enzyme-containing multipurpose feed fermenting agent |
| CN102232466A (en) * | 2010-05-04 | 2011-11-09 | 洋浦慷民高科生物有限公司 | Bio-protein feed prepared by adopting cellulose raw material and preparation method thereof |
| CN102366030A (en) * | 2011-10-25 | 2012-03-07 | 陕西大秦汉集团有限公司 | Mixed composite microbial feed for freshwater fish and production method thereof |
| CN102389035A (en) * | 2011-10-11 | 2012-03-28 | 南京帅丰饲料有限公司 | Preparation of bighead carp biologic feed, and method for feeding bighead carp by using mesh bag |
-
2012
- 2012-04-06 CN CN201210100632.6A patent/CN102630821B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101986862A (en) * | 2009-07-31 | 2011-03-23 | 丁之铨 | Enzyme-containing multipurpose feed fermenting agent |
| CN102232466A (en) * | 2010-05-04 | 2011-11-09 | 洋浦慷民高科生物有限公司 | Bio-protein feed prepared by adopting cellulose raw material and preparation method thereof |
| CN101978850A (en) * | 2010-12-04 | 2011-02-23 | 徐贵阁 | Green biological active feed |
| CN102389035A (en) * | 2011-10-11 | 2012-03-28 | 南京帅丰饲料有限公司 | Preparation of bighead carp biologic feed, and method for feeding bighead carp by using mesh bag |
| CN102366030A (en) * | 2011-10-25 | 2012-03-07 | 陕西大秦汉集团有限公司 | Mixed composite microbial feed for freshwater fish and production method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 吕慧源等: "生物预混料的稳定性研究及应用前景(上)", 《科学养鱼》 * |
| 陈超然等: "在水产养殖中使用微生物活菌制剂应注意的问题", 《养殖与饲料》 * |
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| CN103283961A (en) * | 2013-05-10 | 2013-09-11 | 浙江工业大学 | Method for preparing health-benefiting fermented soybean meal |
| CN103283961B (en) * | 2013-05-10 | 2015-03-04 | 浙江工业大学 | Method for preparing health-benefiting fermented soybean meal |
| CN104686791A (en) * | 2015-02-03 | 2015-06-10 | 苏州亚太星原生物科技有限公司 | Bran treated by microbial fermentation and preparation technology of bran |
| CN106745762A (en) * | 2016-11-28 | 2017-05-31 | 中国水产科学研究院珠江水产研究所 | A kind of region breeding water body miniature organism sealing compound and preparation method thereof |
| CN106745762B (en) * | 2016-11-28 | 2019-09-17 | 中国水产科学研究院珠江水产研究所 | A kind of region breeding water body miniature organism curing agent and preparation method thereof |
| CN108936098A (en) * | 2018-09-14 | 2018-12-07 | 安徽省农业科学院水产研究所 | A kind of production method of the dedicated ecological fermentation feed of Chinese snake head |
| CN108936098B (en) * | 2018-09-14 | 2021-11-23 | 安徽省农业科学院水产研究所 | Production method of special ecological fermented feed for Taiwan loaches |
| CN110150461A (en) * | 2019-07-04 | 2019-08-23 | 徐州工程学院 | A kind of Composite fermentation type microbiological feed and preparation method thereof |
| CN111011665A (en) * | 2019-12-31 | 2020-04-17 | 山西大禹生物工程股份有限公司 | Microbial fermentation wet material for promoting growth of aquatic animals and preparation method and application thereof |
| CN111248365A (en) * | 2020-03-20 | 2020-06-09 | 海南歌颂饲料有限公司 | Feed for improving utilization rate of feed for aquatic young animals and preparation method thereof |
| CN111772058A (en) * | 2020-08-04 | 2020-10-16 | 清远海贝生物技术有限公司 | Active compound feed suitable for aquaculture and preparation method and application thereof |
| CN112456653A (en) * | 2020-10-27 | 2021-03-09 | 广西诺亚生物科技发展有限公司 | Compound for adjusting aquaculture water quality and preparation process thereof |
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