CN110150461A - A kind of Composite fermentation type microbiological feed and preparation method thereof - Google Patents
A kind of Composite fermentation type microbiological feed and preparation method thereof Download PDFInfo
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- CN110150461A CN110150461A CN201910601066.9A CN201910601066A CN110150461A CN 110150461 A CN110150461 A CN 110150461A CN 201910601066 A CN201910601066 A CN 201910601066A CN 110150461 A CN110150461 A CN 110150461A
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- 238000000855 fermentation Methods 0.000 title claims abstract description 32
- 230000004151 fermentation Effects 0.000 title claims abstract description 32
- 230000002906 microbiologic effect Effects 0.000 title claims abstract description 27
- 239000002131 composite material Substances 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 title claims description 21
- 239000001963 growth medium Substances 0.000 claims abstract description 51
- 239000002994 raw material Substances 0.000 claims abstract description 47
- 240000002234 Allium sativum Species 0.000 claims abstract description 24
- 235000004611 garlic Nutrition 0.000 claims abstract description 24
- 241000218628 Ginkgo Species 0.000 claims abstract description 23
- 235000011201 Ginkgo Nutrition 0.000 claims abstract description 23
- 235000008100 Ginkgo biloba Nutrition 0.000 claims abstract description 23
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 21
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 21
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 21
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 13
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 13
- 239000000126 substance Substances 0.000 claims abstract description 8
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 5
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 5
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 5
- 239000007787 solid Substances 0.000 claims description 16
- 241000894006 Bacteria Species 0.000 claims description 12
- 239000002054 inoculum Substances 0.000 claims description 10
- 239000002609 medium Substances 0.000 claims description 10
- 238000010563 solid-state fermentation Methods 0.000 claims description 10
- 241000186660 Lactobacillus Species 0.000 claims description 5
- 230000004913 activation Effects 0.000 claims description 5
- 238000000227 grinding Methods 0.000 claims description 5
- 229940039696 lactobacillus Drugs 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 2
- 230000003519 ventilatory effect Effects 0.000 claims description 2
- 238000005303 weighing Methods 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 1
- 235000007164 Oryza sativa Nutrition 0.000 claims 1
- 235000009566 rice Nutrition 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 10
- 238000000034 method Methods 0.000 abstract description 4
- 230000007812 deficiency Effects 0.000 abstract description 2
- 239000000047 product Substances 0.000 description 14
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 8
- 239000004459 forage Substances 0.000 description 4
- 235000014655 lactic acid Nutrition 0.000 description 4
- 239000004310 lactic acid Substances 0.000 description 4
- 235000015277 pork Nutrition 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000013065 commercial product Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000004224 protection Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 241001071864 Lethrinus laticaudis Species 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- 244000018633 Prunus armeniaca Species 0.000 description 1
- 235000009827 Prunus armeniaca Nutrition 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 238000004500 asepsis Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Mycology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
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Abstract
The present invention relates to technique for producing feed fields, specifically disclose a kind of Composite fermentation type microbiological feed, including strain raw material and culture medium, the strain raw material by bacillus subtilis and lactobacillus plantarum one of or both arbitrary proportion combine；It include dregs of beans, garlic stalk, ginkgo leaf residue and wheat bran in the culture medium, it is dregs of beans 30%-55%, garlic stalk 10%-25%, ginkgo leaf residue 10%-20%, wheat bran 15%-40% that each substance component, which accounts for the weight percentage of culture medium, in the culture medium.The present invention overcomes the deficiencies in the prior art, the garlic stalk for using low cost and ginkgo leaf residue reduce the production cost of feed, meet the needs of industrialization production as fermentation raw material.
Description
Technical field
The present invention relates to technique for producing feed field, a kind of Composite fermentation type microbiological feed and its preparation side are particularly belonged to
Method.
Background technique
Since aquaculture excessively pursues high density, high yield, it is bound to cause breeding pollution object and increases and accumulate, cause to cultivate
The gradually deterioration of environment, major embodiment are as follows: water pollution；Air pollution；Pathogenic microorganism, which is largely bred, to spread the disease；Drug is residual
It stays；Pollution etc. of the microelement excess emissions to environment directly contributes cultivation failure and threatens Product quality and safety.According to current
The national appearance in relation to relevant policies such as food safety, environmental protections, development & production green, efficient, health, nonreactive microorganism
Feed, feed addictive, microbe additive, enzyme preparation become a key subjects of the pendulum in face of current cultivation every profession and trade.
Current microbiological feed is at high cost due to fermentation raw material, causes Feed Manufacturing at high cost, is unfavorable for extensive
Industrialization production is not suitable with the demand in market.
Summary of the invention
The object of the present invention is to provide a kind of Composite fermentation type microbiological feeds, overcome the deficiencies in the prior art, make
The garlic stalk and ginkgo leaf residue for using low cost reduce the production cost of feed, meet industrialization production as fermentation raw material
Demand.
To solve the above problems, the technical solution used in the present invention is as follows:
A kind of Composite fermentation type microbiological feed, including strain raw material and culture medium, the strain raw material is by withered grass gemma
Bacillus and lactobacillus plantarum one of or both arbitrary proportion combine；It include dregs of beans, garlic straw in the culture medium
Stalk, ginkgo leaf residue and wheat bran, it is dregs of beans 30%- that each substance component, which accounts for the weight percentage of culture medium, in the culture medium
55%, garlic stalk 10%-25%, ginkgo leaf residue 10%-20%, wheat bran 15%-40%.
Further, including strain raw material and culture medium, the strain raw material by bacillus subtilis and lactobacillus plantarum it
One of or both arbitrary proportion combine；It include dregs of beans, garlic stalk, ginkgo leaf residue and wheat bran in the culture medium,
It is dregs of beans 41%, garlic stalk 17%, ginkgo leaf residue that each substance component, which accounts for the weight percentage of culture medium, in the culture medium
15%, wheat bran 27%.
Further, the overall viable bacteria content of the strain raw material is 5 × 108-10×109cfu/ml。
A kind of preparation method of Composite fermentation type microbiological feed, it is characterised in that: include the following steps,
(1) preparation of culture medium: claiming according to the weight percentage of dregs of beans in formula, garlic stalk, ginkgo leaf residue and wheat bran
Raw material is taken, above-mentioned raw materials are put into grinding device respectively and are crushed, is uniformly mixed after crossing 40 meshes, solid is made in sterilizing
Culture medium；
(2) actication of culture: being inoculated into LB culture medium for bacillus subtilis and activate, and lactobacillus inoculum to MRS is trained
Feeding base is activated；
(3) strain is inoculated with: the strain raw material activated in step 2 is inoculated into step 1 according to inoculum concentration for 8%-10%
On the solid medium of preparation；
(4) solid state fermentation: inoculated solid medium is placed into Zymolysis Equipment, under the conditions of 30-35 DEG C of temperature,
Continuing fermentation 24-38 hours；
(5) pack: the product fermented in step 4 is dried, is crushed, detect after pack.
Further, the raw material of culture medium described in the step 1 is sterilized using high-temperature steam.
Further, the activation of strain raw material is all made of the mode of inclined-plane culture in the step 2.
Further, in the step 4 solid state fermentation condition are as follows: tank press 004-0.05MPa, speed of agitator 100-
150rpm and ventilatory capacity 1-2vols/vol/min.
Compared with prior art, implementation result of the invention is as follows by the present invention:
1, the garlic stalk and ginkgo leaf residue that the present invention uses low cost greatly reduce microorganism feeding as fermentation raw material
The production cost of material improves the benefit of enterprise, reduces use cost, meet the needs of industrialization production, improves product
Promotional value.
2, the present invention reduces the cost of fermentation by the way of solid fermentation, and fermentation process does not need integral asepsis,
It is easy to operate, meet the requirement of industrialization production.
Specific embodiment
The present invention will be further described with reference to the examples below, but the present invention is not limited to these instances, and is being de-
Under the premise of from present inventive concept, carried out by it is any improvement be within the scope of the present invention.
Embodiment 1
A kind of Composite fermentation type microbiological feed, including strain raw material and culture medium, strain raw material are bacillus subtilis,
The overall viable bacteria content of bacillus subtilis is 5 × 108-10×109cfu/ml；It include dregs of beans, garlic stalk, silver in culture medium
Apricot leaf slag and wheat bran, in culture medium each substance component account for the weight percentage of culture medium be dregs of beans 42%, garlic stalk 12%,
Ginkgo leaf residue 8%, wheat bran 38%.
A kind of preparation method of Composite fermentation type microbiological feed, includes the following steps,
(1) preparation of culture medium: claiming according to the weight percentage of dregs of beans in formula, garlic stalk, ginkgo leaf residue and wheat bran
Raw material is taken, above-mentioned raw materials are put into grinding device respectively and are crushed, is uniformly mixed after crossing 40 meshes, solid is made in sterilizing
Culture medium；
(2) actication of culture: being inoculated into LB culture medium for bacillus subtilis and activate, and lactobacillus inoculum to MRS is trained
Feeding base is activated；
(3) strain is inoculated with: the strain raw material activated in step 2 is inoculated into step 1 according to inoculum concentration for 8%-10%
On the solid medium of preparation；
(4) solid state fermentation: inoculated solid medium is placed into Zymolysis Equipment, under the conditions of 30-35 DEG C of temperature,
Continuing fermentation 24-38 hours；
(5) pack: the product fermented in step 4 is dried, is crushed, detect after pack.
The raw material of culture medium is sterilized using high-temperature steam in step 1；The activation of strain raw material is all made of tiltedly in step 2
The mode of face culture；The condition of solid state fermentation in step 4 are as follows: tank is pressed 004-0.05MPa, speed of agitator 100-150rpm and led to
Tolerance 1-2vols/vol/min.
Thick protein (in terms of dry matter) content reaches 12.9% in microbiological feed made from the present embodiment, effective viable bacteria
Number is 1.25 × 109-1.59×109Cfu/ml, the cost of this product are the 70% of commercially available microbiological feed cost.
Embodiment 2
A kind of Composite fermentation type microbiological feed, including strain raw material and culture medium, strain raw material are lactic acid bacteria, lactic acid bacteria
Overall viable bacteria content be 5 × 108-10×109cfu/ml；It include dregs of beans, garlic stalk, ginkgo leaf residue and wheat bran in culture medium,
In culture medium each substance component account for culture medium weight percentage be dregs of beans 41%, garlic stalk 17%, ginkgo leaf residue 15%,
Wheat bran 27%.
A kind of preparation method of Composite fermentation type microbiological feed, includes the following steps,
(1) preparation of culture medium: claiming according to the weight percentage of dregs of beans in formula, garlic stalk, ginkgo leaf residue and wheat bran
Raw material is taken, above-mentioned raw materials are put into grinding device respectively and are crushed, is uniformly mixed after crossing 40 meshes, solid is made in sterilizing
Culture medium；
(2) actication of culture: being inoculated into LB culture medium for bacillus subtilis and activate, and lactobacillus inoculum to MRS is trained
Feeding base is activated；
(3) strain is inoculated with: the strain raw material activated in step 2 is inoculated into step 1 according to inoculum concentration for 8%-10%
On the solid medium of preparation；
(4) solid state fermentation: inoculated solid medium is placed into Zymolysis Equipment, under the conditions of 30-35 DEG C of temperature,
Continuing fermentation 24-38 hours；
(5) pack: the product fermented in step 4 is dried, is crushed, detect after pack.
The raw material of culture medium is sterilized using high-temperature steam in step 1；The activation of strain raw material is all made of tiltedly in step 2
The mode of face culture；The condition of solid state fermentation in step 4 are as follows: tank is pressed 004-0.05MPa, speed of agitator 100-150rpm and led to
Tolerance 1-2vols/vol/min.
Thick protein (in terms of dry matter) content reaches 10.1% in microbiological feed made from the present embodiment, effective viable bacteria
Number is 0.71 × 109-0.94×109Cfu/ml, the cost of this product are the 61% of commercially available microbiological feed cost.
Embodiment 3
A kind of Composite fermentation type microbiological feed, including strain raw material and culture medium, strain raw material are bacillus subtilis
And lactic acid bacteria, bacillus subtilis and lactic acid bacteria are added according to the ratio of 1:1, the overall viable bacteria content of strain raw material is 5
×108-10×109cfu/ml；It include dregs of beans, garlic stalk, ginkgo leaf residue and wheat bran, each substance group in culture medium in culture medium
The weight percentage that part accounts for culture medium is dregs of beans 31%, garlic stalk 21%, ginkgo leaf residue 19%, wheat bran 29%.
A kind of preparation method of Composite fermentation type microbiological feed, includes the following steps,
(1) preparation of culture medium: claiming according to the weight percentage of dregs of beans in formula, garlic stalk, ginkgo leaf residue and wheat bran
Raw material is taken, above-mentioned raw materials are put into grinding device respectively and are crushed, is uniformly mixed after crossing 40 meshes, solid is made in sterilizing
Culture medium；
(2) actication of culture: being inoculated into LB culture medium for bacillus subtilis and activate, and lactobacillus inoculum to MRS is trained
Feeding base is activated；
(3) strain is inoculated with: the strain raw material activated in step 2 is inoculated into step 1 according to inoculum concentration for 8%-10%
On the solid medium of preparation；
(4) solid state fermentation: inoculated solid medium is placed into Zymolysis Equipment, under the conditions of 30-35 DEG C of temperature,
Continuing fermentation 24-38 hours；
(5) pack: the product fermented in step 4 is dried, is crushed, detect after pack.
The raw material of culture medium is sterilized using high-temperature steam in step 1；The activation of strain raw material is all made of tiltedly in step 2
The mode of face culture；The condition of solid state fermentation in step 4 are as follows: tank is pressed 004-0.05MPa, speed of agitator 100-150rpm and led to
Tolerance 1-2vols/vol/min.
Thick protein (in terms of dry matter) content reaches 13.5% in microbiological feed made from the present embodiment, effective viable bacteria
Number is 1.19 × 109-1.42×109Cfu/ml, the cost of this product are the 53% of commercially available microbiological feed cost.
Embodiment 4
Pork pig culture experiment:
Time: on May 6,6 days to 2019 01 month 2019.
Place: Xuzhou City of Jiangsu Province pork pig farm
Test grouping: selecting in 40 farms the small pork pig that size is identical, is of the similar age, be classified as four groups, point
Not Wei one group, two groups, three groups and control group, one group using the forage feed of 1% embodiment, 1 product is added to, two groups of uses add
The forage feed of 1% embodiment, 2 product is added, three groups are made using the forage feed for being added to 1% embodiment, 3 product, control group
With the forage feed for being added to 1% commercial product, other feeding conditions are all the same.
Test result: after feeding four months, weighing the weight of four groups of piggys respectively, be averaged, and record, concrete outcome
It is shown in Table 1:
| Serial number | Group | Original weight (kg/ is only) | Final weight (kg/ is only) | Average daily gain (kg/ is only) |
| 1 | One group | 40.2 | 126.5 | 0.72 |
| 2 | Two groups | 40.1 | 119.3 | 0.66 |
| 3 | Three groups | 39.2 | 124.1 | 0.71 |
| 4 | Control group | 41.2 | 121.6 | 0.67 |
Interpretation of result: the gaining effect of product of the invention is better than commercial product, and product cost substantially reduces, and meets industry
The demand that metaplasia produces has the value promoted the use of.
Above content is only to present inventive concept example and explanation, affiliated those skilled in the art couple
Described specific embodiment does various modifications or additions or is substituted in a similar manner, without departing from invention
Conceive or beyond the scope defined by this claim, is within the scope of protection of the invention.
Claims (7)
1. a kind of Composite fermentation type microbiological feed, it is characterised in that: including strain raw material and culture medium, the strain raw material by
Bacillus subtilis and lactobacillus plantarum one of or both arbitrary proportion combine；It include beans in the culture medium
The dregs of rice, garlic stalk, ginkgo leaf residue and wheat bran, it is dregs of beans that each substance component, which accounts for the weight percentage of culture medium, in the culture medium
30%-55%, garlic stalk 10%-25%, ginkgo leaf residue 10%-20%, wheat bran 15%-40%.
2. a kind of Composite fermentation type microbiological feed according to claim 1, it is characterised in that: including strain raw material and training
Support base, the strain raw material by bacillus subtilis and lactobacillus plantarum one of or both arbitrary proportion combine；
It include dregs of beans, garlic stalk, ginkgo leaf residue and wheat bran in the culture medium, each substance component accounts for culture medium in the culture medium
Weight percentage is dregs of beans 41%, garlic stalk 17%, ginkgo leaf residue 15%, wheat bran 27%.
3. a kind of Composite fermentation type microbiological feed according to claim 1, it is characterised in that: the strain raw material it is total
Body viable bacteria content is 5 × 108-10×109cfu/ml。
4. a kind of preparation method of Composite fermentation type microbiological feed, it is characterised in that: include the following steps,
(1) preparation of culture medium: weighing original according to the weight percentage of dregs of beans in formula, garlic stalk, ginkgo leaf residue and wheat bran
Material, above-mentioned raw materials are put into grinding device respectively and are crushed, and are uniformly mixed after crossing 40 meshes, and solid culture is made in sterilizing
Base；
(2) actication of culture: being inoculated into LB culture medium for bacillus subtilis and activate, by lactobacillus inoculum to MRS culture medium
It is activated；
(3) strain is inoculated with: the strain raw material activated in step 2 being inoculated into step 1 according to inoculum concentration for 8%-10% and is prepared
Solid medium on；
(4) solid state fermentation: inoculated solid medium is placed into Zymolysis Equipment, under the conditions of 30-35 DEG C of temperature, is continued
Fermentation 24-38 hours；
(5) pack: the product fermented in step 4 is dried, is crushed, detect after pack.
5. a kind of preparation method of Composite fermentation type microbiological feed according to claim 4, it is characterised in that: the step
The raw material of culture medium described in rapid 1 is sterilized using high-temperature steam.
6. a kind of preparation method of Composite fermentation type microbiological feed according to claim 4, it is characterised in that: the step
The activation of strain raw material is all made of the mode of inclined-plane culture in rapid 2.
7. a kind of preparation method of Composite fermentation type microbiological feed according to claim 4, it is characterised in that: the step
The condition of solid state fermentation in rapid 4 are as follows: tank presses 004-0.05MPa, speed of agitator 100-150rpm and ventilatory capacity 1-2vols/
vol/min。
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112998130A (en) * | 2021-04-22 | 2021-06-22 | 沈阳工学院 | Pomace and crop straw mixed fermented feed and preparation method thereof |
| CN115176864A (en) * | 2022-07-04 | 2022-10-14 | 徐州工程学院 | Probiotic fermented compound feed and preparation method thereof |
| CN115553380A (en) * | 2022-10-13 | 2023-01-03 | 徐州工程学院 | Fermented feed and fermentation process thereof |
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