CN102599055B - Quick propagating method for new guinea impatiens - Google Patents
Quick propagating method for new guinea impatiens Download PDFInfo
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- CN102599055B CN102599055B CN 201210070362 CN201210070362A CN102599055B CN 102599055 B CN102599055 B CN 102599055B CN 201210070362 CN201210070362 CN 201210070362 CN 201210070362 A CN201210070362 A CN 201210070362A CN 102599055 B CN102599055 B CN 102599055B
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Abstract
The invention relates to plant cultivation, especially relates to a quick propagating method for new guinea impatiens and provides a tissue culture method for the new guinea impatiens and a method for preparing a fertilizer. The requirements of the tissue culture and differentiation of the new guinea impatiens for various elements in different growth periods are met; the growth and development ofthe roots and leaves of the new guinea impatiens are boosted; a bactericide is added in a formula, so that the functions of sterilizing, killing insects and preventing pest and disease damage are achieved when the fertilizer is applied; and the effect of preventing or relieving the pest and disease damage to the new guinea impatiens is better. According to the method provided by the invention, attapulgite is taken as an adding auxiliary of agricultural fertilizer, and due to the unique physical property of the attapulgite, the metabolism of the plant is promoted and the conversion rate of nutrient substance is increased.
Description
Technical field
The present invention relates to the plant cultivation field, relate in particular to a kind of method for quickly breeding of new guinea impatiens.
Background technology
New guinea impatiens (
Impatiens platypatala) be Balsaminaceae impatiens perennial herb flowers, originate in Asia and the torrid zone, Africa, its breeding center is in the U.S., and the part kind is imported China within this century into, and pattern is very abundant, adularescent, peachiness, redness, purple, orange and secondary color.But blooming in the anniversary mostly, is one of important potted flower of China in recent years.
At present, less for group training test, the fertilizer research of new guinea impatiens, when cultivating new guinea impatiens in the nursery, adopt conventional nitrogen-phosphorus-potassium compound fertilizer more, and in compost, add bone meal etc.The nutrient of these fertilizer is more single, can not satisfy bloom required to various nutritive elements of new guinea impatiens growth, cause new guinea impatiens in the cultivation process phenomenon of oligotrophy, the imbalance of part nutritive element to occur, this can further cause, and bud is few, flower is little, the flower front vane comes off, growth rate is slow, plant is unhealthy and strong etc.; Simultaneously, damage by disease and insect such as the common stem rot of new guinea impatiens, two spotted spider mite, the effect of resisting that when conventional fertilizer is used new guinea impatiens is improved the common disease of this class lacks obvious help, causes damage by disease and insect easily in the course of cultivation.
Summary of the invention
The invention provides a kind of comprehensive nutrition, easy to prepare, new guinea impatiens growth is promoted obviously, pollutes less and to resisting the cultivation method that damage by disease and insect has certain effect.
The present invention adopts following technical scheme: a kind of method for quickly breeding of new guinea impatiens is characterized in that: (1) selects the stem apex of new guinea impatiens for use, with 75% alcohol-pickled 20 minutes, and the clorox sterilization with 9% 10 minutes, aseptic water washing is cleaned.Carry out callus induction with the MS medium, 25 ℃ of temperature, 1800lux illumination is carried out in explant shading 8 days again every day, 10 days generation callus; Adopt the 1/2MS medium to carry out adventitious buds proliferation, 25 ℃ of cultivation temperature, illumination 2800lux cultivated 15 days; The bud of will growing thickly is inoculated on the 1/2MS+IAA0.3mg/L medium, takes root in 18 days for obtaining whole plant, is transplanted in the basin after the hardening.
Soil melioration applies nutrition composite fertilizer, is the prevention soil insect, and every mu applies insecticide Rogor 1.5kg, turn over for the first time, employing is ploughed deeply, and more than the degree of depth 26cm, the dead angle is not stayed in the field, thin rake is once turned over for the second time more then, and bed surface is smooth, the rake of turning over is thin, ditches behind the smooth bed surface, opens gutter around the piece of field, the degree of depth 50～80cm, wide 60cm, central authorities open middle ditch at the field piece, communicate dark 40～60cm, wide 50～60cm with gutter, make bed, the wide 110cm of bed surface, the bed surface soil block is whole thin, bed surface central authorities are high slightly, and both sides are low slightly, open pavement between every seedbed, more than the dark 25cm, wide 40cm, the pavement middle part is high slightly, two is low slightly, guarantees that the ditch ditch communicates the draining smoothness;
(2) transplant and manage: will through the training of the group after hardening transplantation of seedlings to the described seedbed of step (1), transplant the back and will irrigate normal root water the same day, as continuous sunny, to water 1 time water after the transplanting every day, keep ground moistening, as the rainy day, want timely draining, transplant in back 35 days and will set up the sunshade net, light transmittance 60%, full exposure management after 50 days, in addition, in conjunction with fertilising, weed with a hoe, fine behind the rain, in time weed with a hoe, to prevent sealing of soil;
Described nutrition composite fertilizer, each component is by weight: thunder godvine 5-7 part, root bark of shaggy-fruited dittany 2-3 part, trifoliate jewelvine 3-5 part, tobacco leaf 1-3 part, Radix Isatidis 2-3 part, urea 20-25 part, ammonium nitrate 2-6 part, potassium dihydrogen phosphate 1-4 part, potassium sulphate 2-5 part, nitrate of lime 1-2 part, magnesium sulfate 0.5-1 part, borax 1-2 part, zinc sulphate 2-5 part, ferrous sulfate 3-6 part, thiophanate methyl 2-4 part, copper sulphate 1-1.5 part, 3 parts in diatomite, attapulgite 10-15 part, vegetable fiber 11-20 part, fowl and animal excrement 40-60 part, microbial bacterial agent 2-4 part, 5 parts of plant growth regulator.
In the present invention further optimization scheme, the zymotic fluid of the preferred azotobacter of microbial bacterial agent, potassium solubilizing bacteria, phosphate solubilizing bacteria mixes; Described azotobacter be azotobacter chroococcum (
AzotobacterChroococcum), described phosphate solubilizing bacteria be false pseudomonas bacillus (
Pseudomonas), described potassium solubilizing bacteria is silicate bacterium.
In the present invention further optimization scheme, the mixture of a kind of or any several compositions in vegetable fiber preferably wheat stalk, maize straw, cotton stalk, reed rod, sedge, corncob, the bagasse.
In the present invention further optimization scheme, a kind of or any several mixing in the preferred cow dung of fowl and animal excrement, sheep excrement, the chicken and duck excrement;
In the present invention further optimization scheme, a kind of in plant growth regulator preferred oil rape lactone, heteroauxin, the gibberellin.
Fertilizer is prepared as follows in of the present invention:
(1) chooses the raw material of formula ratio by weight.
(2) adopt the particle cracker that vegetable fiber is ground into 50 purpose powder, thunder godvine, the root bark of shaggy-fruited dittany, trifoliate jewelvine, tobacco leaf, Radix Isatidis mixed powder are broken into 20 purpose powder.
(3) fowl and animal excrement, microbial bacterial agent, vegetable fiber powder, attapulgite are mixed, mix thoroughly, the wet feed of in fermentation vat, banking up fermentation, on cover the moisture-heat preservation material, after 50 hours, turning for the first time when temperature reaches 50-70 ℃ in the heap, and every turning in 20-24 hour once, through after 2-3 the turning, fermentation ends.
(4) tunning that step (3) is made mixes with urea, ammonium nitrate, potassium dihydrogen phosphate, potassium sulphate, nitrate of lime, magnesium sulfate, borax, zinc sulphate, ferrous sulfate, mixes thoroughly.
(5) thunder godvine, the root bark of shaggy-fruited dittany, trifoliate jewelvine, tobacco leaf, the Isatis Root after in the mixture of step (4) gained, add pulverizing, and be mixed together evenly with thiophanate methyl, copper sulphate, diatomite and plant growth regulator.
(6) step (5) gained mixture dried, obtain behind the granulating nutrition of the present invention composite fertilizer.
As one of preferred version of the present invention, the moisture of fermenting mixture is controlled at 45%-55%, and C/N ratio is controlled at 20-30:1, and the pH value is controlled at 6.5-7.
As one of present invention further optimization scheme, C/N ratio is controlled at 25:1.
As one of present invention further optimization scheme, the heat and moisture preserving material is Polypropylence Sheet.
As one of present invention further optimization scheme, the interior temperature of heap is 65 ℃ during turning for the first time.
The present invention has following beneficial effect: after new guinea impatiens nutritious fertilizer provided by the present invention is made base manure and once applied, can satisfy the basic nutrition needs of new guinea impatiens whole growth phase, wherein not only contain infrastructure elements such as NPK, also comprised multiple in, trace element and plant growth regulator, new guinea impatiens all can be met in the demand of growth different times for various elements, also help growing of its root of promotion and leaf portion.In addition, added bactericide in the prescription, made fertilizer when applying, also possess the effect of sterilization, desinsection and pre-preventing disease and pest, for control or alleviate the leaf spot that new guinea impatiens may run into, the problem of scale has effect preferably.Simultaneously, adopt attapulgite as the interpolation auxiliary agent of agricultural fertilizer among the present invention, with its distinctive physical property, can promote the metabolism of plant, improve the nutriment conversion ratio.
Use that new guinea impatiens bud behind the fertilizer of the present invention is big and many, beautiful in colour, damage by disease and insect is few, the speed of growing is fast, has favorable economic benefit.
Embodiment
The present invention is described further below by embodiment, but protection scheme of the present invention is not limited to following examples.
Embodiment 1
A kind of cultivation method of new guinea impatiens is characterized in that: (1) soil melioration applies nutrition composite fertilizer, be the prevention soil insect, every mu applies insecticide Rogor 1.5kg, turns over for the first time, employing is ploughed deeply, and more than the degree of depth 26cm, the dead angle is not stayed in the field, thin rake is once turned over for the second time more then, and bed surface is smooth, the rake of turning over is thin, ditches behind the smooth bed surface, opens gutter around the piece of field, the degree of depth 50～80cm, wide 60cm, central authorities open middle ditch at the field piece, communicate dark 40～60cm, wide 50～60cm with gutter, make bed, the wide 110cm of bed surface, the bed surface soil block is whole thin, bed surface central authorities are high slightly, and both sides are low slightly, open pavement between every seedbed, more than the dark 25cm, wide 40cm, the pavement middle part is high slightly, and two is low slightly, guarantee that the ditch ditch communicates the draining smoothness;
(2) transplant and manage: will through the training of the group after hardening transplantation of seedlings to the described seedbed of step (1), transplant the back and will irrigate normal root water the same day, as continuous sunny, to water 1 time water after the transplanting every day, keep ground moistening, as the rainy day, want timely draining, transplant in back 35 days and will set up the sunshade net, light transmittance 60%, full exposure management after 50 days, in addition, in conjunction with fertilising, weed with a hoe, fine behind the rain, in time weed with a hoe, to prevent sealing of soil;
Choose raw material, each component is by weight: 5 parts of thunder godvines, 2 parts of the root bark of shaggy-fruited dittanys, trifoliate jewelvine 3-part, 1 part on tobacco leaf, 2 parts of Radix Isatidis, 20 parts in urea, 2 parts in ammonium nitrate, 1 part of potassium dihydrogen phosphate, 2 parts of potassium sulphates, 1 part in nitrate of lime, 0.5 part in magnesium sulfate, 1 part of borax, 2 parts in zinc sulphate, 3 parts in ferrous sulfate, 2 parts of thiophanate methyls, 1 part in copper sulphate, 3 parts in diatomite, 10 parts of attapulgites, 11 parts of wheat stalks, 40 parts in cattle and sheep excrement mixture, microbial bacterial agent 2-4 part, 5 parts of brassinosteroids.
Adopt the particle cracker that wheat stalk is ground into 50 purpose powder, again thunder godvine, the root bark of shaggy-fruited dittany, trifoliate jewelvine, tobacco leaf, Radix Isatidis mixed powder are broken into 20 purpose powder.
Cow dung, sheep excrement, microbial bacterial agent, wheat stalk powder, attapulgite are mixed, mix thoroughly, the wet feed of in fermentation vat, banking up fermentation, on cover Polypropylence Sheet with moisture-heat preservation, the moisture of fermenting mixture is controlled 45%, after 50 hours, and turning for the first time when temperature reaches 50 ℃ in the heap, and every turning in 20 hours once, after 2 turnings, C/N ratio control is controlled 6.5 fermentation ends in 20:1, pH value.The tunning that makes is mixed with urea, ammonium nitrate, potassium dihydrogen phosphate, potassium sulphate, nitrate of lime, magnesium sulfate, borax, zinc sulphate, ferrous sulfate, mix thoroughly.Thunder godvine, the root bark of shaggy-fruited dittany, trifoliate jewelvine, tobacco leaf, Isatis Root after will pulverizing then and the mixture of just having mixed thoroughly mix, and are mixed together with thiophanate methyl, copper sulphate, diatomite and brassinosteroid to stir again; The end product that obtains is dried, is behind the granulating nutrition of the present invention composite fertilizer.
Thunder godvine in the present embodiment, the root bark of shaggy-fruited dittany, trifoliate jewelvine, tobacco leaf, Radix Isatidis, microbial bacterial agent, attapulgite mixing, urea, ammonium nitrate, potassium dihydrogen phosphate, potassium sulphate, nitrate of lime, magnesium sulfate, borax, zinc sulphate, ferrous sulfate, diatomite, copper sulphate, thiophanate methyl and brassinosteroid all can be buied by market.
Application process:
Select the stem apex of new guinea impatiens for use, with 75% alcohol-pickled 20 minutes, the clorox sterilization with 9% 10 minutes, aseptic water washing is cleaned.Carry out callus induction with the MS medium, 25 ℃ of temperature, 1800lux illumination is carried out in explant shading 8 days again every day, 10 days generation callus; Adopt the 1/2MS medium to carry out adventitious buds proliferation, 25 ℃ of cultivation temperature, illumination 2800lux cultivated 15 days; The bud of will growing thickly is inoculated on the 1/2MS+IAA0.3mg/L medium, takes root in 18 days for obtaining whole plant, is transplanted in the basin after the hardening.
Apply nutrition of the present invention composite fertilizer during transplanting, every strain 0.8kg, earthing waters permeablely, and final-period management is managed according to routine techniques, can bloom then.
Composite fertilizer nutrition composite fertilizer of the present invention and gardening field is commonly used in when fertilising compares test, and 6 processing are set, and each is handled and repeats 3 times, and disposable the applying of each fertilizer employing adopted same control measures, by statistics:
The average number of flowers of using the every strain of new guinea impatiens of conventional composite fertilizer is 15, flowering rate 83.5%, and the growing period normal management, the ratio that infects stem rot is 10.7%, the ratio that infects two spotted spider mite is 15.1%.
The average number of flowers of using the every strain of new guinea impatiens of nutrition of the present invention composite fertilizer reaches 24, and flowering rate reaches 96.9%, and the bright-coloured degree of pattern is higher than the new guinea impatiens that conventional composite fertilizer cultivates.The growing period normal management, the ratio that infects stem rot only is 2.6%, the ratio that infects two spotted spider mite is 4.5%.
Embodiment 2
A kind of cultivation method of new guinea impatiens is characterized in that: (1) soil melioration applies nutrition composite fertilizer, be the prevention soil insect, every mu applies insecticide Rogor 1.5kg, turns over for the first time, employing is ploughed deeply, and more than the degree of depth 26cm, the dead angle is not stayed in the field, thin rake is once turned over for the second time more then, and bed surface is smooth, the rake of turning over is thin, ditches behind the smooth bed surface, opens gutter around the piece of field, the degree of depth 50～80cm, wide 60cm, central authorities open middle ditch at the field piece, communicate dark 40～60cm, wide 50～60cm with gutter, make bed, the wide 110cm of bed surface, the bed surface soil block is whole thin, bed surface central authorities are high slightly, and both sides are low slightly, open pavement between every seedbed, more than the dark 25cm, wide 40cm, the pavement middle part is high slightly, and two is low slightly, guarantee that the ditch ditch communicates the draining smoothness;
(2) transplant and manage: will through the training of the group after hardening transplantation of seedlings to the described seedbed of step (1), transplant the back and will irrigate normal root water the same day, as continuous sunny, to water 1 time water after the transplanting every day, keep ground moistening, as the rainy day, want timely draining, transplant in back 35 days and will set up the sunshade net, light transmittance 60%, full exposure management after 50 days, in addition, in conjunction with fertilising, weed with a hoe, fine behind the rain, in time weed with a hoe, to prevent sealing of soil;
Described nutrition composite fertilizer chooses raw material, 6 parts of thunder godvines, 3 parts of the root bark of shaggy-fruited dittanys, 5 parts of trifoliate jewelvines, 3 parts on tobacco leaf, 2 parts of Radix Isatidis, 23 parts in urea, 5 parts in ammonium nitrate, 2 parts of potassium dihydrogen phosphates, 3 parts of potassium sulphates, 2 parts in nitrate of lime, 1 part in magnesium sulfate, 1 part of borax, 4 parts in zinc sulphate, 5 parts in ferrous sulfate, 3 parts of thiophanate methyls, 1.5 parts in copper sulphate, 3 parts in diatomite, 15 parts of attapulgites, 20 parts of corncobs, 55 parts in chicken and duck excrement, 4 parts of microbial bacterial agents, 5 parts of gibberellin.Adopt the particle cracker that maize cob meal is broken into 60 purpose powder, again thunder godvine, the root bark of shaggy-fruited dittany, trifoliate jewelvine, tobacco leaf, Radix Isatidis mixed powder are broken into 20 purpose powder.
Chicken and duck excrement, microbial bacterial agent, corncob powder, attapulgite are mixed, mix thoroughly, the wet feed of in fermentation vat, banking up fermentation, on cover Polypropylence Sheet with moisture-heat preservation, the moisture of fermenting mixture is controlled 55%, after 50 hours, and turning for the first time when temperature reaches 60 ℃ in the heap, and every turning in 23 hours once, after 3 turnings, C/N ratio control is controlled 7 fermentation ends in 25:1, pH value.The tunning that makes is mixed with urea, ammonium nitrate, potassium dihydrogen phosphate, potassium sulphate, nitrate of lime, magnesium sulfate, borax, zinc sulphate, ferrous sulfate, mix thoroughly.Thunder godvine, the root bark of shaggy-fruited dittany, trifoliate jewelvine, tobacco leaf, Isatis Root after will pulverizing then and the mixture of just having mixed thoroughly mix, and are mixed together with thiophanate methyl, copper sulphate, diatomite and gibberellin to stir again; The end product that obtains is dried, is behind the granulating nutrition of the present invention composite fertilizer.
Thunder godvine in the present embodiment, the root bark of shaggy-fruited dittany, trifoliate jewelvine, tobacco leaf, Radix Isatidis, microbial bacterial agent, attapulgite mixing, urea, ammonium nitrate, potassium dihydrogen phosphate, potassium sulphate, nitrate of lime, magnesium sulfate, borax, zinc sulphate, ferrous sulfate, diatomite, copper sulphate, thiophanate methyl and gibberellin all can be buied by market.
Application process:
Select the stem apex of new guinea impatiens for use, with 75% alcohol-pickled 20 minutes, the clorox sterilization with 9% 10 minutes, aseptic water washing is cleaned.Carry out callus induction with the MS medium, 25 ℃ of temperature, 1800lux illumination is carried out in explant shading 8 days again every day, 10 days generation callus; Adopt the 1/2MS medium to carry out adventitious buds proliferation, 25 ℃ of cultivation temperature, illumination 2800lux cultivated 15 days; The bud of will growing thickly is inoculated on the 1/2MS+IAA0.3mg/L medium, takes root in 18 days for obtaining whole plant, is transplanted in the basin after the hardening.
Apply nutrition of the present invention composite fertilizer during transplanting, every strain 0.9kg, earthing waters permeablely, and final-period management is managed according to routine techniques, can bloom then.
Composite fertilizer nutrition composite fertilizer of the present invention and gardening field is commonly used in when fertilising compares test, and 6 processing are set, and each is handled and repeats 3 times, and disposable the applying of each fertilizer employing adopted same control measures, by statistics:
The average number of flowers of using the every strain of new guinea impatiens of conventional composite fertilizer is 16, flowering rate 85.1%, and the growing period normal management, the ratio that infects stem rot is 9.4%, the ratio that infects two spotted spider mite is 13.6%.
The average number of flowers of using the every strain of new guinea impatiens of nutrition of the present invention composite fertilizer reaches 25, and flowering rate reaches 98.1%, and the bright-coloured degree of pattern is higher than the new guinea impatiens that conventional composite fertilizer cultivates.The growing period normal management, the ratio that infects stem rot only is 2.2%, the ratio that infects two spotted spider mite is 3.9%.
Embodiment 3
A kind of cultivation method of new guinea impatiens is characterized in that: (1) soil melioration applies nutrition composite fertilizer, be the prevention soil insect, every mu applies insecticide Rogor 1.5kg, turns over for the first time, employing is ploughed deeply, and more than the degree of depth 26cm, the dead angle is not stayed in the field, thin rake is once turned over for the second time more then, and bed surface is smooth, the rake of turning over is thin, ditches behind the smooth bed surface, opens gutter around the piece of field, the degree of depth 50～80cm, wide 60cm, central authorities open middle ditch at the field piece, communicate dark 40～60cm, wide 50～60cm with gutter, make bed, the wide 110cm of bed surface, the bed surface soil block is whole thin, bed surface central authorities are high slightly, and both sides are low slightly, open pavement between every seedbed, more than the dark 25cm, wide 40cm, the pavement middle part is high slightly, and two is low slightly, guarantee that the ditch ditch communicates the draining smoothness;
(2) transplant and manage: will through the training of the group after hardening transplantation of seedlings to the described seedbed of step (1), transplant the back and will irrigate normal root water the same day, as continuous sunny, to water 1 time water after the transplanting every day, keep ground moistening, as the rainy day, want timely draining, transplant in back 35 days and will set up the sunshade net, light transmittance 60%, full exposure management after 50 days, in addition, in conjunction with fertilising, weed with a hoe, fine behind the rain, in time weed with a hoe, to prevent sealing of soil;
Described nutrition composite fertilizer chooses raw material, 7 parts of thunder godvines, 3 parts of the root bark of shaggy-fruited dittanys, 5 parts of trifoliate jewelvines, 3 parts on tobacco leaf, 3 parts of Radix Isatidis, 25 parts in urea, 6 parts in ammonium nitrate, 4 parts of potassium dihydrogen phosphates, 5 parts of potassium sulphates, 2 parts in nitrate of lime, 0.5 part in magnesium sulfate, 2 parts of boraxs, 5 parts in zinc sulphate, 6 parts in ferrous sulfate, 4 parts of thiophanate methyls, 1.5 parts in copper sulphate, 3 parts in diatomite, 15 parts of attapulgites, 20 parts of cotton stalks, 60 parts of cow dungs, 4 parts of microbial bacterial agents, 5 parts of heteroauxins.
Cow dung, microbial bacterial agent, cotton stalk powder, attapulgite are mixed, mix thoroughly, the wet feed of in fermentation vat, banking up fermentation, on cover Polypropylence Sheet with moisture-heat preservation, the moisture of fermenting mixture is controlled 53%, after 50 hours, and turning for the first time when temperature reaches 60 ℃ in the heap, and every turning in 24 hours once, after 3 turnings, C/N ratio control is controlled 6.5 fermentation ends in 30:1, pH value.The tunning that makes is mixed with urea, ammonium nitrate, potassium dihydrogen phosphate, potassium sulphate, nitrate of lime, magnesium sulfate, borax, zinc sulphate, ferrous sulfate, mix thoroughly.Thunder godvine, the root bark of shaggy-fruited dittany, trifoliate jewelvine, tobacco leaf, Isatis Root after will pulverizing then and the mixture of just having mixed thoroughly mix, and are mixed together with thiophanate methyl, copper sulphate, diatomite and gibberellin to stir again; The end product that obtains is dried, is behind the granulating nutrition of the present invention composite fertilizer.
Thunder godvine in the present embodiment, the root bark of shaggy-fruited dittany, trifoliate jewelvine, tobacco leaf, Radix Isatidis, microbial bacterial agent, attapulgite mixing, urea, ammonium nitrate, potassium dihydrogen phosphate, potassium sulphate, nitrate of lime, magnesium sulfate, borax, zinc sulphate, ferrous sulfate, diatomite, copper sulphate, thiophanate methyl and heteroauxin all can be buied by market.
Application process:
Select the stem apex of new guinea impatiens for use, with 75% alcohol-pickled 20 minutes, the clorox sterilization with 9% 10 minutes, aseptic water washing is cleaned.Carry out callus induction with the MS medium, 25 ℃ of temperature, 1800lux illumination is carried out in explant shading 8 days again every day, 10 days generation callus; Adopt the 1/2MS medium to carry out adventitious buds proliferation, 25 ℃ of cultivation temperature, illumination 2800lux cultivated 15 days; The bud of will growing thickly is inoculated on the 1/2MS+IAA0.3mg/L medium, takes root in 18 days for obtaining whole plant, is transplanted in the basin after the hardening.
Apply nutritious fertilizer of the present invention during transplanting, every strain 0.7kg, earthing waters permeablely, and final-period management is managed according to routine techniques, can bloom then.
Composite fertilizer nutrition composite fertilizer of the present invention and gardening field is commonly used in when fertilising compares test, and 6 processing are set, and each is handled and repeats 3 times, and disposable the applying of each fertilizer employing adopted same control measures, by statistics:
The average number of flowers of using the every strain of new guinea impatiens of conventional composite fertilizer is about 16, flowering rate 84.6%, and the growing period normal management, the ratio that infects stem rot is 9.9%, the ratio that infects two spotted spider mite is 14.1%.
The average number of flowers of using each inflorescence of new guinea impatiens of nutrition of the present invention composite fertilizer reaches 23, and flowering rate reaches 97.2%, and the bright-coloured degree of pattern is higher than the new guinea impatiens that conventional composite fertilizer cultivates.The growing period normal management, the ratio that infects stem rot only is 3.1%, the ratio that infects two spotted spider mite is 4.4%.
Claims (2)
1. the method for quickly breeding of a new guinea impatiens is characterized in that: (1) tissue is cultivated: select the stem apex of new guinea impatiens for use, and with 75% alcohol-pickled 20 minutes, the clorox sterilization with 9% 10 minutes, aseptic water washing is clean; Carry out callus induction with the MS medium, 25 ℃ of temperature, 1800lux illumination is carried out in explant shading 8 days again every day, 10 days generation callus; Adopt the 1/2MS medium to carry out adventitious buds proliferation, 25 ℃ of cultivation temperature, illumination 2800lux cultivated 15 days; The bud of will growing thickly is inoculated on the 1/2MS+IAA0.3mg/L medium, takes root in 18 days for obtaining whole plant, is transplanted to after the hardening in the basin;
(2) soil melioration applies nutrition composite fertilizer, is the prevention soil insect, and every mu applies insecticide Rogor 1.5kg, turn over for the first time, employing is ploughed deeply, and more than the degree of depth 26cm, the dead angle is not stayed in the field, thin rake is once turned over for the second time more then, and bed surface is smooth, the rake of turning over is thin, ditches behind the smooth bed surface, opens gutter around the piece of field, the degree of depth 50～80cm, wide 60cm, central authorities open middle ditch at the field piece, communicate dark 40～60cm, wide 50～60cm with gutter, make bed, the wide 110cm of bed surface, the bed surface soil block is whole thin, bed surface central authorities are high slightly, and both sides are low slightly, open pavement between every seedbed, more than the dark 25cm, wide 40cm, the pavement middle part is high slightly, two is low slightly, guarantees that the ditch ditch communicates the draining smoothness;
(3) transplant and manage: will through the training of the group after hardening transplantation of seedlings to the described seedbed of step (2), transplant the back and will irrigate normal root water the same day, as continuous sunny, to water 1 time water after the transplanting every day, keep ground moistening, as the rainy day, want timely draining, transplant in back 35 days and will set up the sunshade net, light transmittance 60%, full exposure management after 50 days, in addition, in conjunction with fertilising, weed with a hoe, fine behind the rain, in time weed with a hoe, to prevent sealing of soil;
Described nutrition composite fertilizer, each component is by weight: thunder godvine 5-7 part, root bark of shaggy-fruited dittany 2-3 part, trifoliate jewelvine 3-5 part, tobacco leaf 1-3 part, Radix Isatidis 2-3 part, urea 20-25 part, ammonium nitrate 2-6 part, potassium dihydrogen phosphate 1-4 part, potassium sulphate 2-5 part, nitrate of lime 1-2 part, magnesium sulfate 0.5-1 part, borax 1-2 part, zinc sulphate 2-5 part, ferrous sulfate 3-6 part, thiophanate methyl 2-4 part, copper sulphate 1-1.5 part, 3 parts in diatomite, attapulgite 10-15 part, vegetable fiber 11-20 part, fowl and animal excrement 40-60 part, microbial bacterial agent 2-4 part, 5 parts of plant growth regulator;
Described microbial bacterial agent is mixed by the zymotic fluid of azotobacter, potassium solubilizing bacteria, phosphate solubilizing bacteria; Described azotobacter be azotobacter chroococcum (
AzotobacterChroococcum), described phosphate solubilizing bacteria be false pseudomonas bacillus (
Pseudomonas), described potassium solubilizing bacteria is silicate bacterium;
Described vegetable fiber is a kind of or any several mixing in wheat stalk, maize straw, cotton stalk, reed rod, sedge, corncob, the bagasse;
Described fowl and animal excrement is a kind of or any several mixing in cow dung, sheep excrement, the chicken and duck excrement;
Described plant growth regulator is a kind of in brassinosteroid, heteroauxin, the gibberellin;
In above-mentioned, the preparation method of described nutrition composite fertilizer the steps include:
(1) chooses the raw material of formula ratio by weight;
(2) vegetable fiber is ground into 50-60 purpose powder, thunder godvine, the root bark of shaggy-fruited dittany, trifoliate jewelvine, tobacco leaf, Radix Isatidis mixed powder are broken into 20 purpose powder;
(3) fowl and animal excrement, microbial bacterial agent, vegetable fiber powder, attapulgite are mixed, mix thoroughly, the wet feed of in fermentation vat, banking up fermentation, on cover the moisture-heat preservation material, after 50 hours, turning for the first time when temperature reaches 50-70 ℃ in the heap, and every turning in 20-24 hour once, through after 2-3 the turning, fermentation ends; (4) tunning that step (3) is made mixes with urea, ammonium nitrate, potassium dihydrogen phosphate, potassium sulphate, nitrate of lime, magnesium sulfate, borax, zinc sulphate, ferrous sulfate, mixes thoroughly; (5) thunder godvine, the root bark of shaggy-fruited dittany, trifoliate jewelvine, tobacco leaf, the Isatis Root after in the mixture of step (4) gained, add pulverizing, and be mixed together evenly with thiophanate methyl, copper sulphate, diatomite and plant growth regulator; (6) step (5) gained mixture dried, obtain behind the granulating nutrition of the present invention composite fertilizer.
2. the method for quickly breeding of new guinea impatiens according to claim 1 is characterized in that, the moisture control of fermenting mixture is at 45%-55% in the step (3) among the preparation method of described nutrition composite fertilizer, and C/N ratio is controlled at 20-30:1, and the pH value is controlled at 6.5-7.
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| CN110384042A (en) * | 2019-06-26 | 2019-10-29 | 西南林业大学 | A kind of new guinea impatiens tissue culture and rapid propagation method |
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| JPH05244824A (en) * | 1991-12-17 | 1993-09-24 | Idemitsu Kosan Co Ltd | Method for culturing plant |
| JP2000287535A (en) * | 1999-04-06 | 2000-10-17 | Takeshi Mori | Multiplication of upright raising of impatiens sultanii (impatiens) |
| US6924416B2 (en) * | 1999-12-28 | 2005-08-02 | Ball Horticultural Company | Trailing interspecific impatiens |
| CN1405124A (en) * | 2001-08-12 | 2003-03-26 | 王秀波 | Biofermented compound fertilizer composition and its production method |
| CN1168379C (en) * | 2002-05-31 | 2004-09-29 | 华南师范大学 | Quick breeding method of impatiens new-guianiense |
| CN1216013C (en) * | 2002-07-01 | 2005-08-24 | 左广胜 | Composite microbial bacteria adsorbent |
| CN100496216C (en) * | 2007-02-16 | 2009-06-10 | 北京林业大学 | NPK nutrition diagnosis method of pot flower Impatiens hawkeri |
| JPWO2010047349A1 (en) * | 2008-10-21 | 2012-03-22 | 日本ゼオン株式会社 | Polymerizable composition, resin molded body, laminate, and dielectric device |
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