CN102578013A - Method for preventing and treating black spermatophore disease of Litopenaeus vannamei - Google Patents

Method for preventing and treating black spermatophore disease of Litopenaeus vannamei Download PDF

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Publication number
CN102578013A
CN102578013A CN2012100702984A CN201210070298A CN102578013A CN 102578013 A CN102578013 A CN 102578013A CN 2012100702984 A CN2012100702984 A CN 2012100702984A CN 201210070298 A CN201210070298 A CN 201210070298A CN 102578013 A CN102578013 A CN 102578013A
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spermatophore
black
environment
litopenaeus vannamei
water
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梁华芳
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Guangdong Ocean University
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Guangdong Ocean University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

The invention provides a method for preventing and treating black spermatophore disease of Litopenaeus vannamei, falling into the field of aquaculture disease prevention and treatment. The method is mainly realized through technical measures including aquaculture water treatment, aquaculture environment control, feeding of high-quality baits, drug application and artificial black spermatophore removal by extrusion. The method has convenient application, curative rate up to above 90%, low death rate of male prawn, and no need of cooperation with ripened female prawn. Female prawn ripening and spawning can be performed after re-culture and ripening of diseased male prawn. The method can realize reasonable production arrangement and save production cost.

Description

A kind of sick method of the black spermatophore of Environment of Litopenaeus vannamei Low of preventing and treating
Technical field
The present invention relates to a kind of sick method of the black spermatophore of Environment of Litopenaeus vannamei Low of preventing and treating, belong to aquatic products disease control field.
Background technology
Black spermatophore disease is meant the spermatophore blackening of the male shrimp of Environment of Litopenaeus vannamei Low, and the sperm fertilizing ability in the spermatophore is low or lose a kind of disease of fertility.After ill, it is poor that the sperm in the black spermatophore is not had fertility or a fertilization ability, causes Environment of Litopenaeus vannamei Low artificial propagation failure.
At present, black spermatophore sick mainly through with the method for the female shrimp mating of maturation, migrate out from spermatophore sac deceiving spermatophore in the male shrimp, form new spermatophore again at spermatophore sac, thereby reach the purpose of control.But this method needs ripe female shrimp to be cooperated; Because sperm fertilizing ability is poor in the black spermatophore, the ovum of output be nonfertilization or fertilization rate extremely low, and male shrimp post-coitum; Again form spermatophore and need the 8d left and right sides time to maturation; During this period, ripe 1-2 time of female shrimp possibility has been wasted the ovum that female shrimp produced; Incur loss through delay the production time simultaneously, consumed water power, manpower, the energy (heating) etc., improved production cost greatly.Therefore, need not use under the prerequisite of the female shrimp of accelerating the ripening, the male shrimp that suffers from black spermatophore is being cured, could guarantee carrying out smoothly of artificial propagation, practice thrift cost.
Summary of the invention
The objective of the invention is to have expensive deficiency to the generality and the sick existing method of preventing and treating of the black spermatophore of Environment of Litopenaeus vannamei Low of the sick morbidity of the black spermatophore of Environment of Litopenaeus vannamei Low; Provide a kind of through handle to cultivate water, control nurturing an environment, the high-quality of throwing something and feeding bait; Drug administration and the artificial comprehensive technical measure of extruding black spermatophore are prevented and treated the sick method of the black spermatophore of Environment of Litopenaeus vannamei Low.
In order to address the above problem; The technical scheme that the present invention adopted is following: a kind of sick method of the black spermatophore of Environment of Litopenaeus vannamei Low of preventing and treating comprises following technical measures: handle and cultivate water, control nurturing an environment, the high-quality of throwing something and feeding bait, drug administration and manual work extrude black spermatophore.
The method that water is cultivated in said processing is: extract the natural waters seawater, the natural waters seawater is used further to suffer from the cultivation of the sick Environment of Litopenaeus vannamei Low of black spermatophore after through the husky filter of one-level, chlorine-containing disinfectant sterilization, the husky filter of secondary.
The method of said control nurturing an environment is: keep salinity 28-35, illumination 200-500lx, pH are 7.9-8.2, water temperature 28-29 ℃; Total ammonia nitrogen < 0.5 mg/>L, nitrite nitrogen < 0.1 mg/>L.
Said high-quality bait be in four types of clam worm, shellfish, squid and the swimming crabs more than 3 kinds or 3 kinds; Feeding method is: the above-mentioned high-quality bait that will choose every day is thrown something and fed once for every kind; Wherein the clam worm feeding volume accounts for 30% of total feeding volume, and the bait of all the other kinds accounts for 70% of total feeding volume.
The method of said drug administration is: at the bottom of the pond, pond is cultivated in cleaning with soil pick-up, change water after the chlorine dioxide 1-1.5mL/m of application concentration 5% in cultivating water body immediately 3, after the chlorine dioxide 6h of application concentration 5%, in cultivating water body, use Norfloxacin 2 mg/L again; The chlorine dioxide of concentration 5% is used once every other day, and continuous administration 7-10 days, Norfloxacin was used once every day, and continuous administration 7-10 days, said two kinds of medicines were when using on the same day, and must be 6h blanking time.
Said manual work is extruded black spermatophore and is meant: when cultivate water through handling, control nurturing an environment, the high-quality of throwing something and feeding bait, four kinds of technical measures of drug administration handles; Also there is the black spermatophore Environment of Litopenaeus vannamei Low of not curing; Then by hand method or electric shocking method extruded its black spermatophore; The Environment of Litopenaeus vannamei Low behind the black spermatophore is extruded in acquisition; By handle cultivating water, controlling nurturing an environment, after the technical measures of the high-quality of throwing something and feeding bait handle, the Environment of Litopenaeus vannamei Low of extruding behind the black spermatophore cultivated, formed the high spermatophore of sperm quality again through 7-10 days again.
Said chlorine-containing disinfectant is a kind of in clorox, chloride of lime, calcium hypochlorite, the sodium trichloro-isocyanurate.
Said clam worm is multiple tooth clam worm or the perinereis aibihitensis Grube of enclosing, and said shellfish is pleat oyster or Ruditapes philippinarum.
Manual work according to the invention is extruded black spermatophore and is meant that manual method or electric shocking method extrude black spermatophore.Manual method is extruded black spermatophore method: 2 people operation, and a people puts on cotton gloves, catches the disease shrimp, exposes the spermatophore position, and another human thumb will be deceived spermatophore and from gonopore, released gently; Electric shocking method takes out black spermatophore method: prepare one of charger; Call in the test test pencil that two red-black universal meters are used, be linked on the charger both positive and negative polarity, be transferred to 3A and 4V to current/voltage; The negative pole pen is placed on abdominal ganglion; Anodal pen is placed on the below of black spermatophore, shocks by electricity 1-2 second, and black spermatophore is gone out from gonopore; If black spermatophore is not hit, available manual method will be deceived spermatophore and released gently.
It is sick that the present invention is used to prevent and treat the black spermatophore of Environment of Litopenaeus vannamei Low, but inapplicable to black spermatophore downright bad, that form lump.
The present invention with respect to the beneficial effect of prior art is: the method convenient in application of preventing and treating of the present invention; The sick cure rate of the black spermatophore of Environment of Litopenaeus vannamei Low is reached more than 90%; Male shrimp lethality is low, does not need ripe female shrimp to cooperate, and can the male shrimp of deceiving spermatophore be cultivated maturation again and carry out the cultivation of accelerating the ripening of female shrimp again; The method of preventing and treating of the present invention can realize the reasonable arrangement to producing simultaneously, saves production cost.
Embodiment
Through embodiment the present invention is explained further details below, these embodiment only are used for explaining the present invention, do not limit the scope of the invention.
Embodiment 1: according to the method for preventing and treating of the present invention, adopt following technical measures that the black spermatophore disease of Environment of Litopenaeus vannamei Low is prevented and treated.
1. cultivate and use water treatment
(1) the husky filter of one-level: the seawater of natural waters is drawn up, filter through the one-level sand filter, i.e. coarse filtration, this grade filtering ponds can be contained in the sea, also can be contained in above the land, mainly play thick foreign material and the mud effect of intercepting;
(2) chlorine-containing disinfectant sterilization, deposition: the seawater that will pass through the husky filter of one-level is put in the sedimentation basin, adds clorox, makes that effective chlorine density reaches 10 mg/L in the water, adds in an amount of sodium thiosulfate behind the 24h and chlorine residue, aeration 24h;
(3) carry out the husky filter of secondary: will sterilize through chlorine-containing disinfectant, the seawater of post precipitation is through carrying out the husky filter of secondary, accomplishes the processing of cultivating water, obtains the secondary sand filter water; The sand of secondary sand filter requires delicate, and preferably can adorn the active carbon of 30 cm thicks, to obtain better filtered water.
2. the cultivation of the male shrimp of black spermatophore: the secondary sand filter water that will obtain after will handling is introduced and is cultivated the pond, and the male shrimp that will suffer from black spermatophore disease again moves to cultivate in the pond cultivates processing, in cultivating process, adopts following technical measures to deceive the sick control of spermatophore:
(1) control nurturing an environment: control environment the factor in following scope through changing measures such as water, shading, temperature control: keep salinity 28, illumination 300-350lx, pH are 8.0,28 ℃ of water temperatures; Total ammonia nitrogen < 0.5 mg/>L, nitrite nitrogen < 0.1 mg/>L;
(2) the high-quality bait of throwing something and feeding: throw something and feed every day and multiple toothly enclose clam worm once, the pleat oyster once, squid once, every kind of bait accounts for 30%, 35% and 35% of total feeding volume;
(3) at the bottom of soil pick-up and the clear pond, change water: change water 50% every day, cleaning is cultivated at the bottom of the pond, pond and soil pick-up 2 times, keeps the cleaning at the bottom of the pond;
(4) water body is cultivated in drug administration sterilization: at the bottom of the pond, pond is cultivated in cleaning with soil pick-up, change water after during in 10:00 in cultivating water body the chlorine dioxide 1mL/m of application concentration 5% 3, behind the chlorine dioxide 6h of application concentration 5%, when 16:00, use Norfloxacin 2 mg/L; 5% chlorine dioxide is used once every other day, continuous administration 7 days, and Norfloxacin is used once every day, continuous administration 7 days; 5% chlorine dioxide and Norfloxacin when using on the same day, administration interval time 6h;
(5) black spermatophore is extruded in manual work: adopt the measure of above-mentioned (1)-(5) to handle after 7 days, also have the male shrimp of the black spermatophore of curing, then method will deceive that spermatophore is extruded or electric shocking method will be deceived spermatophore and taken out by hand;
(6) extrude the cultivation of the male shrimp behind the black spermatophore
1. control nurturing an environment: control environment the factor in following scope through changing measures such as water, shading, temperature control: keep salinity 32, illumination 300-350lx, pH are 8.0,28 ℃ of water temperatures; Total ammonia nitrogen < 0.5 mg/>L, nitrite nitrogen < 0.1 mg/>L;
2. the high-quality of throwing something and feeding bait: throw something and feed every day and multiple toothly enclose clam worm once, the pleat oyster once, squid once accounts for 30%, 40% and 30% of total feeding volume respectively;
3. at the bottom of soil pick-up and the clear pond, change water: change water 50% every day, cleaning is cultivated at the bottom of the pond, pond and soil pick-up 2 times, keeps the cleaning at the bottom of the pond.
Through cultivation in 8 days, can will deceive the male shrimp of spermatophore and cure cure rate 92%.
Embodiment 2: according to the method for preventing and treating of the present invention, adopt following technical measures that the black spermatophore disease of Environment of Litopenaeus vannamei Low is prevented and treated.
1. cultivate and use water treatment
(1) the husky filter of one-level: the seawater of natural waters is drawn up, filter through the one-level sand filter, i.e. coarse filtration, this grade filtering ponds can be contained in the sea, also can be contained in above the land, mainly play thick foreign material and the mud effect of intercepting;
(2) chlorine-containing disinfectant sterilization, deposition: the seawater that will pass through the husky filter of one-level is put in the sedimentation basin, adds sodium trichloro-isocyanurate, makes that effective chlorine density reaches 10 mg/L in the water, adds in an amount of sodium thiosulfate behind the 24h and chlorine residue, aeration 24h;
(3) carry out the husky filter of secondary: will sterilize through chlorine-containing disinfectant, the seawater of post precipitation is through carrying out the husky filter of secondary, accomplishes the processing of cultivating water, obtains the secondary sand filter water; The sand of secondary sand filter requires delicate, and preferably can adorn the active carbon of 30 cm thicks, to obtain better filtered water.
2. the cultivation of the male shrimp of black spermatophore: the secondary sand filter water that will obtain after will handling is introduced and is cultivated the pond, and the male shrimp that will suffer from black spermatophore disease again moves to cultivate in the pond cultivates processing, in cultivating process, adopts following technical measures to deceive the sick control of spermatophore:
(1) control nurturing an environment: control environment the factor in following scope through changing measures such as water, shading, temperature control: keep salinity 35, illumination 500lx, pH are 8.0,28 ℃ of water temperatures; Total ammonia nitrogen < 0.5 mg/>L, nitrite nitrogen < 0.1 mg/>L;
(2) the high-quality bait of throwing something and feeding: throw something and feed perinereis aibihitensis Grube every day once, the pleat oyster once, squid once, the off-lying sea swimming crab once, the ratio that feeding volume accounts for total feeding volume respectively is 30%, 35%, 25%, 10%;
(3) at the bottom of soil pick-up and the clear pond, change water: change water 50% every day, cleaning is cultivated at the bottom of the pond, pond and soil pick-up 1 time, keeps the cleaning at the bottom of the pond;
(4) water body is cultivated in drug administration sterilization: at the bottom of the pond, pond is cultivated in cleaning with soil pick-up, change water after during in 9:00 in cultivating water body the chlorine dioxide 1.5mL/m of application concentration 5% 3, behind the chlorine dioxide 6h of application concentration 5%, use Norfloxacin 2 mg/L in 15:00; 5% chlorine dioxide is used once every other day, continuous administration 8 days; Norfloxacin is used once every day, continuous administration 8 days, 5% chlorine dioxide and Norfloxacin when using on the same day, administration interval time 6h;
(5) black spermatophore is extruded in manual work: adopt the measure of above-mentioned (1)-(5) to handle after 8 days, if also have the male shrimp of the black spermatophore of curing, then method will deceive that spermatophore is extruded or electric shocking method will be deceived spermatophore and taken out by hand;
(6) extrude the cultivation of the male shrimp behind the black spermatophore
1. control nurturing an environment: control environment the factor in following scope through changing measures such as water, shading, temperature control: keep salinity 35, illumination 500lx, pH are 8.2,28 ℃ of water temperatures; Total ammonia nitrogen < 0.5 mg/>L, nitrite nitrogen < 0.1 mg/>L;
2. the high-quality of throwing something and feeding bait: throw something and feed perinereis aibihitensis Grube every day once, Ruditapes philippinarum once, squid once accounts for 30%, 40% and 30% of total feeding volume respectively;
3. at the bottom of soil pick-up and the clear pond, change water: change water 50% every day, cleaning is cultivated at the bottom of the pond, pond and soil pick-up 2 times, keeps the cleaning at the bottom of the pond.
Through cultivation in 10 days, can will deceive the male shrimp of spermatophore and cure, cure rate is 93%.
Embodiment 3: according to the method for preventing and treating of the present invention, adopt following technical measures that the black spermatophore disease of Environment of Litopenaeus vannamei Low is prevented and treated.
1. cultivate and use water treatment
(1) the husky filter of one-level: the seawater of natural waters is drawn up, filter through the one-level sand filter, i.e. coarse filtration, this grade filtering ponds can be contained in the sea, also can be contained in above the land, mainly play thick foreign material and the mud effect of intercepting;
(2) chlorine-containing disinfectant sterilization, deposition: the seawater that will pass through the husky filter of one-level is put in the sedimentation basin, adds chloride of lime, makes that effective chlorine density reaches 10 mg/L in the water, adds in an amount of sodium thiosulfate behind the 24h and chlorine residue, aeration 24h;
(3) carry out the husky filter of secondary: will sterilize through chlorine-containing disinfectant, the seawater of post precipitation is through carrying out the husky filter of secondary, accomplishes the processing of cultivating water, obtains the secondary sand filter water; The sand of secondary sand filter requires delicate, and preferably can adorn the active carbon of 30 cm thicks, to obtain better filtered water.
2. the cultivation of the male shrimp of black spermatophore: the secondary sand filter water that will obtain after will handling is introduced and is cultivated the pond, and the male shrimp that will suffer from black spermatophore disease again moves to cultivate in the pond cultivates processing, in cultivating process, adopts following technical measures to deceive the sick control of spermatophore:
(1) control nurturing an environment: control environment the factor in following scope through changing measures such as water, shading, temperature control: keep salinity 30, illumination 400lx, pH are 8.2,29 ℃ of water temperatures; Total ammonia nitrogen < 0.5 mg/>L, nitrite nitrogen < 0.1 mg/>L;
(2) the high-quality bait of throwing something and feeding: throw something and feed perinereis aibihitensis Grube every day once, Ruditapes philippinarum once, squid once, the off-lying sea swimming crab once accounts for 30%, 30%, 30% and 10% of total feeding volume respectively;
(3) at the bottom of soil pick-up and the clear pond, change water: change water 60% every day, cleaning is cultivated at the bottom of the pond, pond and soil pick-up 2 times, keep the cleaning at the bottom of the pond;
(4) water body is cultivated in drug administration sterilization: at the bottom of the pond, pond is cultivated in cleaning with soil pick-up, change water after during in 8:00 in cultivating water body the chlorine dioxide 1.2mL/m of application concentration 5% 3, behind the chlorine dioxide 6h of application concentration 5%, when 14:00, use Norfloxacin 2 mg/L; The chlorine dioxide of concentration 5% is used once every other day, continuous administration 10 days, Norfloxacin is used once every day, continuous administration 10 days, the chlorine dioxide of concentration 5% and Norfloxacin when using on the same day, administration interval time 6h;
(5) black spermatophore is extruded in manual work: adopt the measure of above-mentioned (1)-(5) to handle after 10 days, if also have the male shrimp of the black spermatophore of curing, then method will deceive that spermatophore is extruded or electric shocking method will be deceived spermatophore and taken out by hand;
(6) extrude the cultivation of the male shrimp behind the black spermatophore
1. control nurturing an environment: control environment the factor in following scope through changing measures such as water, shading, temperature control: keep salinity 30, illumination 400lx, pH are 8.0,29 ℃ of water temperatures; Total ammonia nitrogen < 0.5 mg/>L, nitrite nitrogen < 0.1 mg/>L;
2. the high-quality of throwing something and feeding bait: throw something and feed perinereis aibihitensis Grube every day once, Ruditapes philippinarum once, squid once, the off-lying sea swimming crab once, every kind of bait accounts for 30%, 30%, 30% and 10% of total feeding volume;
3. at the bottom of soil pick-up and the clear pond, change water: change water 60% every day, cleaning is cultivated at the bottom of the pond, pond and soil pick-up 1 time, keeps the cleaning at the bottom of the pond.
Through cultivation in 7 days, can will deceive the male shrimp of spermatophore and cure cure rate 90%.
Embodiment 4 adopts following technical measures that the black spermatophore disease of Environment of Litopenaeus vannamei Low is prevented and treated according to the method for preventing and treating of the present invention.
1. cultivate and use water treatment
(1) the husky filter of one-level: the seawater of natural waters is drawn up, filter through the one-level sand filter, i.e. coarse filtration, this grade filtering ponds can be contained in the sea, also can be contained in above the land, mainly play thick foreign material and the mud effect of intercepting;
(2) chlorine-containing disinfectant sterilization, deposition: the seawater that will pass through the husky filter of one-level is put in the sedimentation basin, adds calcium hypochlorite, makes that effective chlorine density reaches 10 mg/L in the water, adds in an amount of sodium thiosulfate behind the 24h and chlorine residue, aeration 24h;
(3) carry out the husky filter of secondary: will sterilize through chlorine-containing disinfectant, the seawater of post precipitation is through carrying out the husky filter of secondary, accomplishes the processing of cultivating water, obtains the secondary sand filter water; The sand of secondary sand filter requires delicate, and preferably can adorn the active carbon of 30 cm thicks, to obtain better filtered water.
2. the cultivation of the male shrimp of black spermatophore: the secondary sand filter water that will obtain after will handling is introduced and is cultivated the pond, and the male shrimp that will suffer from black spermatophore disease again moves to cultivate in the pond cultivates processing, in cultivating process, adopts following technical measures to deceive the sick control of spermatophore:
(1) control nurturing an environment: control environment the factor in following scope through changing measures such as water, shading, temperature control: keep salinity 30, illumination 200-300lx, pH are 8.0,29 ℃ of water temperatures; Total ammonia nitrogen < 0.5 mg/>L, nitrite nitrogen < 0.1 mg/>L;
(2) the high-quality bait of throwing something and feeding: throw something and feed every day and multiple toothly enclose clam worm once, the pleat oyster once, squid once, the off-lying sea swimming crab once accounts for 30%, 20%, 20% and 30% of total feeding volume respectively;
(3) at the bottom of soil pick-up and the clear pond, change water: change water 80% every day, cleaning is cultivated at the bottom of the pond, pond and soil pick-up 2 times, keeps the cleaning at the bottom of the pond;
(4) water body is cultivated in drug administration sterilization: at the bottom of the pond, pond is cultivated in cleaning with soil pick-up, change water after the chlorine dioxide 1.4mL/m of application concentration 5% in cultivating water body immediately 3, use Norfloxacin 2 mg/L behind the chlorine dioxide 6h of application concentration 5%; The chlorine dioxide of concentration 5% is used once every other day, continuous administration 9 days, Norfloxacin is used once every day, continuous administration 9 days, the chlorine dioxide of concentration 5% and Norfloxacin when using on the same day, administration interval time 6h;
(5) black spermatophore is extruded in manual work: adopt the measure of above-mentioned (1)-(5) to handle after 10 days, if also have the male shrimp of the black spermatophore of curing, then method will deceive that spermatophore is extruded or electric shocking method will be deceived spermatophore and taken out by hand;
(6) extrude the cultivation of the male shrimp behind the black spermatophore
1. control nurturing an environment: control environment the factor in following scope through changing measures such as water, shading, temperature control: keep salinity 31, illumination 200-300lx, pH are 8.0,29 ℃ of water temperatures; Total ammonia nitrogen < 0.5 mg/>L, nitrite nitrogen < 0.1 mg/>L;
2. the high-quality of throwing something and feeding bait: throw something and feed every day and multiple toothly enclose clam worm once, the pleat oyster once, squid once, the off-lying sea swimming crab once, every kind of bait accounts for 30%, 25%, 25% and 20% of total feeding volume;
3. at the bottom of soil pick-up and the clear pond, change water: change water 80% every day, cleaning is cultivated at the bottom of the pond, pond and soil pick-up 1 time, keeps the cleaning at the bottom of the pond.
Through cultivation in 9 days, can will deceive the male shrimp of spermatophore and cure cure rate 94%.

Claims (8)

1. prevent and treat the sick method of the black spermatophore of Environment of Litopenaeus vannamei Low for one kind, it is characterized in that: this method comprises following technical measures: handle and cultivate water, control nurturing an environment, the high-quality of throwing something and feeding bait, drug administration and manual work extrude black spermatophore.
2. the sick method of the black spermatophore of Environment of Litopenaeus vannamei Low of preventing and treating according to claim 1; It is characterized in that: the method that water is cultivated in said processing is: extract the natural waters seawater, the natural waters seawater is used further to suffer from the cultivation of the sick black spermatophore Environment of Litopenaeus vannamei Low of black spermatophore after through the husky filter of one-level, chlorine-containing disinfectant sterilization, the husky filter of secondary.
3. the sick method of the black spermatophore of Environment of Litopenaeus vannamei Low of preventing and treating according to claim 1, it is characterized in that: the method for said control nurturing an environment is: keep salinity 28-35, illumination 200-500lx, pH are 7.9-8.2, water temperature 28-29 ℃; Total ammonia nitrogen < 0.5 mg/>L, nitrite nitrogen < 0.1 mg/>L.
4. the sick method of the black spermatophore of Environment of Litopenaeus vannamei Low of preventing and treating according to claim 1; It is characterized in that: said high-quality bait be in four types of clam worm, shellfish, squid and the swimming crabs more than 3 kinds or 3 kinds; Feeding method is: the above-mentioned high-quality bait that will choose every day is thrown something and fed once for every kind; Wherein the clam worm feeding volume accounts for 30% of total feeding volume, and the bait of all the other kinds accounts for 70% of total feeding volume.
5. the sick method of the black spermatophore of Environment of Litopenaeus vannamei Low of preventing and treating according to claim 1, it is characterized in that: the method for said drug administration is: at the bottom of the pond, pond is cultivated in cleaning with soil pick-up, change water after the chlorine dioxide 1-1.5mL/m of application concentration 5% in cultivating water body immediately 3, after the chlorine dioxide 6h of application concentration 5%, in cultivating water body, use Norfloxacin 2 mg/L again; The chlorine dioxide of concentration 5% is used once every other day, and continuous administration 7-10 days, Norfloxacin was used once every day, and continuous administration 7-10 days, said two kinds of medicines were when using on the same day, and must be 6h blanking time.
6. the sick method of the black spermatophore of Environment of Litopenaeus vannamei Low of preventing and treating according to claim 1; It is characterized in that: said manual work is extruded black spermatophore and is meant: when handling through the said method of claim 2-5; Also there is the black spermatophore Environment of Litopenaeus vannamei Low of not curing; Then by hand method or electric shocking method extruded its black spermatophore, obtains to extrude the Environment of Litopenaeus vannamei Low behind the black spermatophore, again by the said method of claim 2-4; Environment of Litopenaeus vannamei Low to extruding behind the black spermatophore was cultivated, and formed the high spermatophore of sperm quality again through 7-10 days.
7. the method for water is cultivated in processing according to claim 2, it is characterized in that: said chlorine-containing disinfectant is a kind of in clorox, chloride of lime, calcium hypochlorite, the sodium trichloro-isocyanurate.
8. high-quality bait according to claim 4 is characterized in that: said clam worm is multiple tooth clam worm or the perinereis aibihitensis Grube of enclosing, and said shellfish is pleat oyster or Ruditapes philippinarum.
CN2012100702984A 2012-03-16 2012-03-16 Method for preventing and treating black spermatophore disease of Litopenaeus vannamei Pending CN102578013A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105532537A (en) * 2016-01-05 2016-05-04 中国水产科学研究院淡水渔业研究中心 Method for extracting macrobrachium spermatophore through pulse electric shock method and macrobrachium intraspecific crossbreeding method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105532537A (en) * 2016-01-05 2016-05-04 中国水产科学研究院淡水渔业研究中心 Method for extracting macrobrachium spermatophore through pulse electric shock method and macrobrachium intraspecific crossbreeding method

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Application publication date: 20120718