CN105532537A - Method for extracting macrobrachium spermatophore through pulse electric shock method and macrobrachium intraspecific crossbreeding method - Google Patents
Method for extracting macrobrachium spermatophore through pulse electric shock method and macrobrachium intraspecific crossbreeding method Download PDFInfo
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- CN105532537A CN105532537A CN201610005939.6A CN201610005939A CN105532537A CN 105532537 A CN105532537 A CN 105532537A CN 201610005939 A CN201610005939 A CN 201610005939A CN 105532537 A CN105532537 A CN 105532537A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a method for extracting macrobrachium spermatophore through a pulse electric shock method and a macrobrachium intraspecific crossbreeding method. The methods comprise the steps that two pulse electrode tips are put on the portions close to the rear sides of two fifth paraeiopod bases, a connecting lead between the two electrode tips is parallel to the junction loop line of the cephalothorax and the abdomen of male macrobrachium, the electrode tips are basically perpendicular to the abdomen surface of the male macrobrachium, a pulse power switch is gently clicked once or twice, and the male macrobrachium is simulated through pulse electricity; a person pays close attention to spermatophore discharging holes formed in the inner sides of the fifth paraeiopod bases, takes down the spermatophore as soon as finding the spermatophore with ophthalmological tweezers and rapidly stick the spermatophore to the portions between the fourth paraeiopod bases and the fifth paraeiopod bases of congener female macrobrachium which just finishes reproductive uncoating, and the female macrobrachium conducts oviposition and incubation by itself. According to the method for extracting the macrobrachium spermatophore through the pulse electric shock method and the macrobrachium intraspecific crossbreeding method, the spermatophore can be extracted from the male macrobrachium rapidly, effectively and safely, the pulse electricity is adopted to extract the spermatophore, the male macrobrachium is not damaged, and compared with stimulation through an ordinary alternating current, the extracting success rate, the transplanting success rate and the fertilization rate are all obviously increased.
Description
Technical field
The invention belongs to aquatic livestock breeding and breeding technical field, relate to test-tube committed step in the crossbreeding of pond crayfish class, particularly relate to the technology that electric pulse stimulation extracts the male shrimp spermatophore of pond crayfish class.
Background technology
The important economic shrimps of pond crayfish Lei Shi China, the freshwater shrimp especially in pond crayfish and Macrobrachium rosenbergii are the important freshwater aquiculture shrimps of China.Announce according to " China Fisheries yearbook ", China freshwater shrimps in 2013 and Macrobrachium rosenbergii cultivation annual production are respectively 25.1 and 11.7 ten thousand tons. and cultivation province reaches 21 and 16 respectively, but along with the continuous expansion of cultivation scale, improving constantly of intensive degree, all there is serious deterioration of variety phenomenon, individuality diminishes, and disease increases, and culture benefit glides year by year.Deterioration of variety has become the bottleneck of pond crayfish class aquaculture, seriously constrains the development of its aquaculture, in the urgent need to carrying out genetic improvement to it, cultivates the new varieties that economic characters are excellent.Selection cross is effective traditional breeding method means.The hybridization of pond crayfish class needs to extract ripe spermatophore from the male shrimp of maturation, and extracting spermatophore is the earliest adopt to kill the method that shrimp gets essence, and so male shrimp can only be used once.Sandifer in 1980 etc. extract Macrobrachium rosenbergii spermatophore with low-voltage alternating-current electrical stimulation and obtain successfully; Qiu Gaofeng in 1997 extracts freshwater shrimp spermatophore with the low-voltage AC of 4-6V and obtains successfully, and the spermatophore extracted by the method is transplanted to 16 and has completed in the female shrimp of freshwater shrimp of reproductive moult, only has 10 to lay eggs smoothly, produce shrimp feritilization of ovum rate 71%.
Summary of the invention
The object of the invention is to provide a kind of efficient, reliable and the undamaged Pulse Electric striking of male shrimp is extracted to method and the pond crayfish class intraspecific hybridization breeding method of pond crayfish class spermatophore for the defect of prior art.
The present invention for achieving the above object, adopts following technical scheme:
Pulse Electric striking extracts a method for pond crayfish class spermatophore, it is characterized in that comprising the steps:
(1) two Pulse Electric cartridges are placed near on rear side of two the 5th step base portions, make the line between two electrode tips parallel with belly connecting portion loop wire with male shrimp cephalothorax, make electrode tip substantially vertical with the outside of belly of male shrimp, dub pulse power switch 1 to 2 time, by Pulse Electric, male shrimp is stimulated;
(2) keep a close eye on ejaculation hole inside male shrimp the 5th step base portion, find that spermatophore is taken the photograph son with ophthalmology immediately and taken off.
It is further characterized in that: described electrode tip is the cylindrical copper that head is spherical, copper wire diameter Ф 2mm, and the long 17mm of electrode tip, rear portion 15mm cover has insulating sheath, and head exposes 2mm, two electrode tip spacing 6mm.
Preferred: described Pulse Electric parameter is that pulse voltage controls at 30 ~ 35V, pulse width 30 ~ 70 μ S.
When water temperature 20 DEG C ~ 30 DEG C, the time interval again obtaining normal mature spermatophore is 48-72 hour.
A kind of pond crayfish class intraspecific hybridization breeding method, it is characterized in that the pond crayfish class spermatophore comprising the steps: the method adopting above-mentioned Pulse Electric striking to extract pond crayfish class spermatophore to extract, Rapid pasting, to of the same race female shrimp the 4th, the 5th step base portion central authorities just completing reproduction shelling, allows female shrimp lay eggs voluntarily, to hatch.
The present invention can fast effectively and extracted from male shrimp by spermatophore safely, it adopts Pulse Electric to extract spermatophore, not only to male shrimp not damaged, and success rate of extracting, transplanting succeed rate and fertilization rate etc. are all significantly increased than common alternating current stimulation, the spermatophore success rate of extracting more than 93% of ripe male shrimp, the success rate of spermatophore transplant experiment and produce shrimp feritilization of ovum rate all more than 82%.Spermatophore is extracted by the method, survival rate and the undressed male shrimp of the male shrimp processed do not have difference, and when water temperature 20 DEG C ~ 30 DEG C, interval 48-72 hour stimulates again, can again obtain normal mature spermatophore, and spermatophore recovery rate, spermatophore transplanting succeed rate and fertilization rate and for the first time equal indifference.
Accompanying drawing explanation
Fig. 1 is electrode tip schematic diagram of the present invention.
Embodiment
Concrete technical scheme of the present invention is as follows:
1) electrical stimulation Special electric cartridge 1: as shown in Figure 1, electrode tip 1 is made by cylindrical copper, the thick Ф 2mm of copper cash, copper cash extension 17mm, the overwhelming majority (15mm) is protected with insulating sheath 2, only exposes head about 2mm, top ends does globulate, the distance 6mm between two electrode tips 1.
2) Pulse Electric parameter is determined: pulse electricity generator can be made by oneself, also can with other cell electro' asion instrument or electroporation apparatus etc., pulse voltage controls at 30 ~ 35V, pulse width 30 ~ 70 μ S, suitably can adjust by the kind of pond crayfish and Individual Size during concrete operations, general individual is larger, shell is thicker, and pulse voltage and the pulse width of use are larger.
3) spermatophore extracts method of operating: left hand holds shrimp, use left hand thumb, forefinger clamps shrimp body cephalothorax, middle finger, shrimp body afterbody is held in nameless and little finger of toe and the centre of the palm, its fixing unlikely stretching is made to bend jump, electrical stimulation Special electric cartridge is placed near on rear side of two the 5th step base portions by the right hand, make the line between two electrode tips parallel with belly connecting portion loop wire with male shrimp cephalothorax, make electrode tip and the outside of belly substantially vertical, electrode can be held with left hand thumb and middle finger during one man operation simultaneously, dub pulse power switch 1 to 2 time, keep a close eye on ejaculation hole inside male shrimp the 5th step base portion simultaneously, find that spermatophore is taken the photograph son with ophthalmology immediately and taken off.
4) pond crayfish class intraspecific hybridization breeding method: the pond crayfish class spermatophore that the method adopting above-mentioned Pulse Electric striking to extract pond crayfish class spermatophore is extracted, Rapid pasting, to of the same race female shrimp the 4th, the 5th step base portion central authorities (paste front blotting paper and blot water) just completing reproduction shelling, allows female shrimp lay eggs voluntarily, to hatch.Statistics spermatophore transplanting succeed rate and fertilization rate.
Embodiment 1:
Freshwater shrimp spermatophore extracts and transplants: the electrode tip using the present invention's design, and use 30V pulse voltage, pulse width 35 μ S, spermatophore recovery rate 96%, spermatophore is transplanted to the success rate 87% on female freshwater shrimp body, fertilization rate 92%.
Embodiment 2:
Macrobrachium hainanense spermatophore extracts and transplants: the electrode tip using the present invention's design, and use 31V pulse voltage, pulse width 40 μ S, spermatophore recovery rate 94%, spermatophore is transplanted to the success rate 85% on female Macrobrachium hainanense body, fertilization rate 90.5%.
Embodiment 3:
Macrobrachium rosenbergii spermatophore extracts and transplants: the electrode tip using the present invention's design, and use 34V pulse voltage, pulse width 60 μ S, spermatophore recovery rate 93%, spermatophore is transplanted to the success rate 83% on female Macrobrachium rosenbergii body, fertilization rate 89%.
Above embodiment only illustrates the pond crayfish spermatophore extracting method that the present invention sets up and pond crayfish class intraspecific hybridization breeding method, but the present invention is not limited to this.It should be noted that and extract the method for spermatophore all within protection domain of the presently claimed invention by electric pulse stimulation, no longer other specific embodiment is enumerated here.
Claims (5)
1. Pulse Electric striking extracts a method for pond crayfish class spermatophore, it is characterized in that comprising the steps:
(1) two Pulse Electric cartridges are placed near on rear side of two the 5th step base portions, make the line between two electrode tips parallel with belly connecting portion loop wire with male shrimp cephalothorax, make electrode tip substantially vertical with the outside of belly of male shrimp, dub pulse power switch 1 to 2 time, by Pulse Electric, male shrimp is stimulated;
(2) keep a close eye on ejaculation hole inside male shrimp the 5th step base portion, find that spermatophore is taken the photograph son with ophthalmology immediately and taken off.
2. Pulse Electric striking according to claim 1 extracts the method for pond crayfish class spermatophore, it is characterized in that: described electrode tip is the cylindrical copper that head is spherical, copper wire diameter Ф 2mm, the long 17mm of electrode tip, 15mm cover in rear portion has insulating sheath, and head exposes 2mm, two electrode tip spacing 6mm.
3. Pulse Electric striking according to claim 1 and 2 extracts the method for pond crayfish class spermatophore, it is characterized in that: described Pulse Electric parameter is that pulse voltage controls at 30 ~ 35V, pulse width 30 ~ 70 μ S.
4. Pulse Electric striking according to claim 3 extracts the method for pond crayfish class spermatophore, it is characterized in that: when water temperature 20 DEG C ~ 30 DEG C, and the time interval again obtaining normal mature spermatophore is 48-72 hour.
5. a pond crayfish class intraspecific hybridization breeding method, it is characterized in that the pond crayfish class spermatophore comprising the steps: the method that the Pulse Electric striking adopted described in any one of claim 1-3 extracts pond crayfish class spermatophore to extract, Rapid pasting, to of the same race female shrimp the 4th, the 5th step base portion central authorities just completing reproduction shelling, allows female shrimp lay eggs voluntarily, to hatch.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610005939.6A CN105532537A (en) | 2016-01-05 | 2016-01-05 | Method for extracting macrobrachium spermatophore through pulse electric shock method and macrobrachium intraspecific crossbreeding method |
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| CN201610005939.6A CN105532537A (en) | 2016-01-05 | 2016-01-05 | Method for extracting macrobrachium spermatophore through pulse electric shock method and macrobrachium intraspecific crossbreeding method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109874712A (en) * | 2019-03-07 | 2019-06-14 | 广西壮族自治区水产科学研究院 | A kind of inseminatio externalis method of litopenaeus vannamei |
| CN113288502A (en) * | 2021-05-27 | 2021-08-24 | 山东省海洋生物研究院 | Device is transplanted to shrimp spermatophore |
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| CN102077793A (en) * | 2010-12-08 | 2011-06-01 | 厦门大学 | Method for replanting waste spermatophore of marsupenaeus japonicus |
| CN102578013A (en) * | 2012-03-16 | 2012-07-18 | 广东海洋大学 | Method for preventing and treating black spermatophore disease of Litopenaeus vannamei |
| CN104737953A (en) * | 2015-03-27 | 2015-07-01 | 深圳市龙科源水产养殖有限公司 | Synchronous decortication method for lobsters |
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2016
- 2016-01-05 CN CN201610005939.6A patent/CN105532537A/en active Pending
Patent Citations (3)
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| CN102077793A (en) * | 2010-12-08 | 2011-06-01 | 厦门大学 | Method for replanting waste spermatophore of marsupenaeus japonicus |
| CN102578013A (en) * | 2012-03-16 | 2012-07-18 | 广东海洋大学 | Method for preventing and treating black spermatophore disease of Litopenaeus vannamei |
| CN104737953A (en) * | 2015-03-27 | 2015-07-01 | 深圳市龙科源水产养殖有限公司 | Synchronous decortication method for lobsters |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109874712A (en) * | 2019-03-07 | 2019-06-14 | 广西壮族自治区水产科学研究院 | A kind of inseminatio externalis method of litopenaeus vannamei |
| CN109874712B (en) * | 2019-03-07 | 2021-04-06 | 广西壮族自治区水产科学研究院 | In-vitro fertilization method for litopenaeus vannamei |
| CN113288502A (en) * | 2021-05-27 | 2021-08-24 | 山东省海洋生物研究院 | Device is transplanted to shrimp spermatophore |
| CN113288502B (en) * | 2021-05-27 | 2022-09-09 | 山东省海洋生物研究院 | Device is transplanted to shrimp spermatophore |
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Application publication date: 20160504 |