CN110663702B - Preparation for preventing and treating ciliate of Eriocheir sinensis - Google Patents
Preparation for preventing and treating ciliate of Eriocheir sinensis Download PDFInfo
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- CN110663702B CN110663702B CN201910955307.XA CN201910955307A CN110663702B CN 110663702 B CN110663702 B CN 110663702B CN 201910955307 A CN201910955307 A CN 201910955307A CN 110663702 B CN110663702 B CN 110663702B
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- 241000223782 Ciliophora Species 0.000 title claims abstract description 54
- 238000002360 preparation method Methods 0.000 title claims abstract description 39
- 241000371997 Eriocheir sinensis Species 0.000 title claims abstract description 34
- YRIZYWQGELRKNT-UHFFFAOYSA-N 1,3,5-trichloro-1,3,5-triazinane-2,4,6-trione Chemical compound ClN1C(=O)N(Cl)C(=O)N(Cl)C1=O YRIZYWQGELRKNT-UHFFFAOYSA-N 0.000 claims abstract description 34
- 229950009390 symclosene Drugs 0.000 claims abstract description 34
- RZLVQBNCHSJZPX-UHFFFAOYSA-L zinc sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Zn+2].[O-]S([O-])(=O)=O RZLVQBNCHSJZPX-UHFFFAOYSA-L 0.000 claims abstract description 24
- 230000002147 killing effect Effects 0.000 claims abstract description 18
- 239000005944 Chlorpyrifos Substances 0.000 claims description 48
- SBPBAQFWLVIOKP-UHFFFAOYSA-N chlorpyrifos Chemical compound CCOP(=S)(OCC)OC1=NC(Cl)=C(Cl)C=C1Cl SBPBAQFWLVIOKP-UHFFFAOYSA-N 0.000 claims description 48
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 34
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 28
- 229960001763 zinc sulfate Drugs 0.000 claims description 28
- 239000000835 fiber Substances 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 17
- 230000000694 effects Effects 0.000 claims description 16
- 238000002156 mixing Methods 0.000 claims description 7
- 239000004033 plastic Substances 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 6
- 238000007865 diluting Methods 0.000 claims description 3
- 239000002023 wood Substances 0.000 claims description 3
- 239000003139 biocide Substances 0.000 claims 2
- 239000003527 fibrinolytic agent Substances 0.000 claims 1
- 230000003480 fibrinolytic effect Effects 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 12
- 230000006378 damage Effects 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 4
- 208000027418 Wounds and injury Diseases 0.000 abstract description 2
- 208000014674 injury Diseases 0.000 abstract description 2
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 27
- 238000012360 testing method Methods 0.000 description 18
- 239000003814 drug Substances 0.000 description 7
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 6
- 229910001385 heavy metal Inorganic materials 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 241001113556 Elodea Species 0.000 description 5
- 244000236458 Panicum colonum Species 0.000 description 5
- 235000015225 Panicum colonum Nutrition 0.000 description 5
- 238000009360 aquaculture Methods 0.000 description 5
- 244000144974 aquaculture Species 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- 244000025254 Cannabis sativa Species 0.000 description 4
- 239000004480 active ingredient Substances 0.000 description 4
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 4
- 229910000365 copper sulfate Inorganic materials 0.000 description 4
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 4
- 241000411851 herbal medicine Species 0.000 description 4
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 4
- 241000195493 Cryptophyta Species 0.000 description 3
- 241000238557 Decapoda Species 0.000 description 3
- 241000498251 Hydrilla Species 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000002195 synergetic effect Effects 0.000 description 3
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
- 241000168525 Haematococcus Species 0.000 description 2
- 239000005843 Thiram Substances 0.000 description 2
- 239000011717 all-trans-retinol Substances 0.000 description 2
- 235000019169 all-trans-retinol Nutrition 0.000 description 2
- 238000004061 bleaching Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000000645 desinfectant Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 210000002816 gill Anatomy 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 239000012286 potassium permanganate Substances 0.000 description 2
- 239000013049 sediment Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- KUAZQDVKQLNFPE-UHFFFAOYSA-N thiram Chemical compound CN(C)C(=S)SSC(=S)N(C)C KUAZQDVKQLNFPE-UHFFFAOYSA-N 0.000 description 2
- 229960002447 thiram Drugs 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 description 1
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 description 1
- 239000005499 Clomazone Substances 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 239000005802 Mancozeb Substances 0.000 description 1
- 240000000233 Melia azedarach Species 0.000 description 1
- 208000030852 Parasitic disease Diseases 0.000 description 1
- 241000201377 Radopholus Species 0.000 description 1
- 244000299790 Rheum rhabarbarum Species 0.000 description 1
- 235000009411 Rheum rhabarbarum Nutrition 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 239000003619 algicide Substances 0.000 description 1
- JBDGDEWWOUBZPM-XYPYZODXSA-N ambroxol Chemical compound NC1=C(Br)C=C(Br)C=C1CN[C@@H]1CC[C@@H](O)CC1 JBDGDEWWOUBZPM-XYPYZODXSA-N 0.000 description 1
- 229960005174 ambroxol Drugs 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- BLUAFEHZUWYNDE-NNWCWBAJSA-N artemisinin Chemical compound C([C@](OO1)(C)O2)C[C@H]3[C@H](C)CC[C@@H]4[C@@]31[C@@H]2OC(=O)[C@@H]4C BLUAFEHZUWYNDE-NNWCWBAJSA-N 0.000 description 1
- 229960004191 artemisinin Drugs 0.000 description 1
- 229930101531 artemisinin Natural products 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 235000009120 camo Nutrition 0.000 description 1
- FAKRSMQSSFJEIM-RQJHMYQMSA-N captopril Chemical compound SC[C@@H](C)C(=O)N1CCC[C@H]1C(O)=O FAKRSMQSSFJEIM-RQJHMYQMSA-N 0.000 description 1
- 229960000830 captopril Drugs 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 235000005607 chanvre indien Nutrition 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- KIEDNEWSYUYDSN-UHFFFAOYSA-N clomazone Chemical compound O=C1C(C)(C)CON1CC1=CC=CC=C1Cl KIEDNEWSYUYDSN-UHFFFAOYSA-N 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 239000011487 hemp Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000000077 insect repellent Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229910052745 lead Inorganic materials 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000033116 oxidation-reduction process Effects 0.000 description 1
- 230000024241 parasitism Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Inorganic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a preparation for preventing and controlling ciliates of eriocheir sinensis, which consists of 180-210 parts by weight of zinc sulfate heptahydrate and 75-100 parts by weight of trichloroisocyanuric acid. The invention has the advantages of no injury to aquatic weeds, safety and stability, and good ciliate killing effect on the premise of reducing the use concentration of effective substances.
Description
Technical Field
The invention relates to the technical field of aquaculture production, in particular to a preparation for preventing and controlling ciliates of eriocheir sinensis.
Background
Ciliate diseases are common parasitic diseases in aquaculture and are also frequently caused in the cultivation process of eriocheir sinensis, and the ciliate diseases of shrimp and crab are caused by the attachment of ciliates such as radopholus, polyworm, bell worm and the like on the body surfaces of the shrimp and crab, including eyes and gills. The pond with overlarge stocking density, poor seedling quality, excessive organic matters at the bottom of the pond, eutrophication of water bodies and frequent abuse of microbial preparations has high probability of occurrence of the disease. Ciliate parasitism can cause the phenomena of unshelling and death of eriocheir sinensis, further the growth speed is reduced, and individuals on the market are smaller or die.
At present, the medicines for preventing and controlling ciliates in aquaculture mainly comprise: 1. zinc sulfate, copper sulfate and its compound preparation, 2, potassium permanganate, 3, formaldehyde or glutaraldehyde, 4, dithiocyano methane solution, 5, mancozeb preparation (zineb, thiram, etc.), 6, chinese herbal medicine preparation, such as artemisinin, chinaberry, rhubarb, kuh-seng, etc.
Because the Eriocheir sinensis is cultivated in Zhejiang zone in grass cultivation (commonly known as 'crab size, water grass viewing', and waterweed, bitter grass and black algae are generally planted), the Eriocheir sinensis has the effects of being used as feed, preventing mutual residue and shading, and the like, the basic requirement for using medicines in the crab pond is that the water grass is not damaged, and the crab pond is safe and effective and allowed to be used in China. The above 6 general formulations have the following problems: 1. zinc sulfate, copper sulfate and its compound preparation are used as a kind of heavy metal salt pesticide as the primary medicine for resisting ciliate in aquaculture industry. But ciliates develop resistance to it because of no alternative product and long term use. Copper sulfate and the preparation thereof are used as algicides and have great harm to aquatic weeds, so the copper sulfate and the preparation thereof are rarely used for Eriocheir sinensis cultivation. 2. The potassium permanganate, formaldehyde or glutaraldehyde and dithiocyano methane solution have the function of killing sessile ciliates, however, the medicines are easy to form algae pouring or massive death of microorganisms after being used in normal doses, aldehydes cause the death of pasture and water due to yellow rot, the water quality is black and odorous, and the death is easy to be caused by the stimulation of gill parts of eriocheir sinensis. Therefore, the 3-class product is rarely used in Eriocheir sinensis cultivation. 3. The ambroxol preparation (zineb, thiram and the like) is a pesticide, has excellent killing effect on ciliates, but is forbidden to be used in aquaculture in China due to toxicity, residue and the like. 4. The Chinese herbal medicine preparation has the characteristics of natural and environment-friendly, but researches show that the Chinese herbal medicine preparation has a large dosage for achieving a good treatment effect, and the Chinese herbal medicine preparation can only take effect when the splashing concentration reaches 15mg/L by taking radix sophorae flavescentis powder as an example, so that the use of the finished product is too high, and the popularization and the application of the product in the cultivation process are limited.
As early as 1998, the U.S. environmental protection agency evaluated the sediment of five lagoons, and the investigation report indicated that the sediment of water body generally had heavy metal pollution problems, mainly including heavy metals such as Pb, cd and Hg, and eight kinds of heavy metals as main pollutants in 2010, namely As, cd, cr, cu, pb, hg, ni and Zn. Although zinc sulfate is used as an insect repellent, the environmental hazard of heavy metals is not ignored, and the dosage of zinc sulfate can be reduced under the action of the same curative effect, so that the zinc sulfate is worthy of deep research and innovation by aquatic science and technology workers.
The trichloroisocyanuric acid has strong sterilization and bleaching effects and quick-acting slow-release effects, and can be used as a washing bleaching agent for livestock breeding industry, cotton and hemp fabrics, a wool shrink-proof agent, a rubber chlorination, a battery material, an organic synthesis industry, dry bleaching of clothes and the like. Trichloroisocyanuric acid is a water disinfectant, and has no killing effect on ciliates.
Disclosure of Invention
The invention aims to provide a preparation for preventing ciliate of Eriocheir sinensis, which has the advantages of no injury to aquatic weeds, safety and stability, and good ciliate killing effect on the premise of reducing the use concentration of effective substances.
The technical scheme adopted for solving the technical problems is as follows:
a preparation for preventing and controlling ciliate of Eriocheir sinensis consists of 180-210 parts by weight of zinc sulfate heptahydrate and 75-100 parts by weight of trichloroisocyanuric acid.
A method for using preparation for preventing and treating ciliate of Eriocheir sinensis comprises dissolving trichloroisocyanuric acid in water, adding zinc sulfate heptahydrate, and mixing.
A preparation for preventing and treating ciliate of Eriocheir sinensis comprises 60-70wt% of fiber and 30-40wt% of chlorpyrifos, wherein the sum of the two is 100%.
Preferably, the composition is formed by mixing the fiber and the chlorpyrifos, wherein the weight percentage of the fiber is 65-66.7%, the weight percentage of the chlorpyrifos is 33.3-35%, and the sum of the two is 100%.
The zinc sulfate heptahydrate content in the fiber is 60%.
The content of trichloroisocyanuric acid in the chlorpyrifos is 50%.
A method for using preparation for preventing and treating ciliate of Eriocheir sinensis comprises dissolving chlorpyrifos in water, and adding gram fiber to obtain uniform mixture.
The active ingredient in the fiber is zinc sulfate heptahydrate, and the active ingredient in the chlorpyrifos is trichloroisocyanuric acid; the scheme of the invention can be directly formed by combining zinc sulfate heptahydrate and trichloroisocyanuric acid, and can also be formed by combining commercially available products of captopril and chlorpyrifos.
The clever (zinc sulfate heptahydrate) is widely used as a ciliate killing medicament for a long time, and has no harm to aquatic weeds, but the ciliate has obvious drug resistance in the state, so that the use amount of the clever is greatly increased to meet the same killing effect as the prior art, and the heavy metal hazard is increased. Chlorpyrifos (trichloroisocyanuric acid) is a water disinfectant which has no killing effect on ciliates. The inventor finds that the cleft (zinc sulfate heptahydrate) and the chlorpyrifos (trichloroisocyanuric acid) are used simultaneously in a long-term research process, the two can play a special synergistic effect, the chlorpyrifos (trichloroisocyanuric acid) can obviously improve the killing effect of the cleft (zinc sulfate heptahydrate), so that the ciliate killing effect can be improved under the condition that the dosage of the cleft (zinc sulfate heptahydrate) is not increased, the dosage of the cleft (zinc sulfate heptahydrate) can be effectively reduced, the chlorpyrifos (trichloroisocyanuric acid) does not hurt water grass and eriocheir sinensis, and the water body can be disinfected, and the combination of the chlorpyrifos (trichloroisocyanuric acid) and the Chinese eriocheir sinensis is safe and stable. The invention utilizes trichloroisocyanuric acid to destroy the cell walls of the ciliates, plays a role in permeation and synergism, and enhances the use effect of zinc sulfate.
The beneficial effects of the invention are as follows: the use cost is low, the components are safe to people and livestock under the condition of effective use concentration, the aquatic weeds and the eriocheir sinensis are not damaged, and the ciliate killing effect is good on the premise of reducing the use concentration of effective substances.
Detailed Description
The technical scheme of the invention is further specifically described by the following specific examples.
In the present invention, the materials and equipment used are commercially available or commonly used in the art, unless otherwise specified. The methods in the following examples are conventional in the art unless otherwise specified.
Example 1:
a preparation for preventing and treating ciliate of Eriocheir sinensis comprises 180g zinc sulfate heptahydrate and 100g trichloroisocyanuric acid.
Example 2:
a preparation for preventing and treating ciliate of Eriocheir sinensis comprises 210g zinc sulfate heptahydrate and 75g trichloroisocyanuric acid.
Example 3:
a preparation for preventing and treating ciliate of Eriocheir sinensis comprises zinc sulfate heptahydrate 200g and trichloroisocyanuric acid 90 g.
Example 4:
a preparation for preventing and treating ciliate of Eriocheir sinensis comprises 180g zinc sulfate heptahydrate and 75g trichloroisocyanuric acid.
The application method of the embodiment 1-4 comprises the steps of adding 5L of water into trichloroisocyanuric acid, adding zinc sulfate heptahydrate, uniformly mixing, and then putting into a crab pond for use, wherein the dosage of the pond is 1m deep water and 1.5 mu deep water, and the mixed solution is used.
Example 5:
a preparation for preventing and treating ciliate of Eriocheir sinensis comprises a fiber-forming agent (commercially available) with zinc sulfate heptahydrate content of 60% and a chlorpyrifos (commercially available) with trichloroisocyanuric acid content of 50%, wherein the weight of the fiber-forming agent is 300g, and the weight of the chlorpyrifos is 200g.
Example 6:
a preparation for preventing and treating ciliate of Eriocheir sinensis comprises (by weight) kefenflur (commercially available) with zinc sulfate heptahydrate content of 60% and chlorpyrifos (commercially available) with trichloroisocyanuric acid content of 50% (by weight), wherein the weight of kefenflur is 350g, and the weight of chlorpyrifos is 150g.
Example 7:
a preparation for preventing and treating ciliate of Eriocheir sinensis comprises a fiber-forming agent (commercially available) with zinc sulfate heptahydrate content of 60% and a chlorpyrifos (commercially available) with trichloroisocyanuric acid content of 50%, wherein the weight of the fiber-forming agent is 325g, and the weight of the chlorpyrifos is 175g.
Example 8:
a preparation for preventing and treating ciliate of Eriocheir sinensis comprises a fiber-obtaining (commercially available) with zinc sulfate heptahydrate content of 60% and a chlorpyrifos (commercially available) with trichloroisocyanuric acid content of 50%, wherein the weight of the fiber-obtaining is 333.5 g, and the weight of the chlorpyrifos is 166.5g.
The application method of the embodiment 5-8 comprises the steps of adding 5L of water into chlorpyrifos, adding fiber into the chlorpyrifos to obtain uniform mixture, and putting the mixture into a crab pond for use, wherein the dosage of the pond is 1m deep and 1.5 mu deep.
Test section:
test 1: the optimization of the component proportion of the preparation and the use method have the effect of comparing that the common preparation for preventing and treating ciliate of Eriocheir sinensis on the current market is zinc sulfate, such as 'keqinde' produced by Wuxi Zhongyi and company, the main component is 60 percent zinc sulfate heptahydrate, and the main component of 'qinling Jing' produced by Shanxi shrimp An aquatic pharmaceutical industry Co., ltd is 60 percent zinc sulfate heptahydrate. The main component of the "clomazone" produced by Shanxiety and Chang corporation is 50% trichloroisocyanuric acid, and the above products are all veterinary medicine GMP products. The comparative analysis of the preparation was carried out using the above-mentioned product as a raw material, wherein zinc sulfate was selected as "keshen".
1. Calculation of ciliate removal Rate with Zinc sulfate at different concentrations
Since trichloroisocyanuric acid alone is ineffective in killing ciliates, no relevant report has been made. The report of killing ciliates by zinc sulfate is more, so the project group performs LD on the toxicity of zinc sulfate to ciliates 50 The test zinc sulfate was calculated to provide a "crochet" source using concentrations of 0.1ppm,0.2ppm,0.3ppm,0.4ppm,0.5ppm,0.6ppm,0.7ppm,0.8ppm. Test ciliate crab 20 per group, test conditions: the size of the water cluster box is 100cm multiplied by 80cm, the test water is pond water, the test water depth is 50cm, the water body is 0.5 cubic meter, the test site is in the fresh water aquatic product institute of Zhejiang province, the test water temperature is 24 ℃, and the test water is pH 7.8. After 48 hours, the existence of ciliates on the crab body surface is observed by a microscope.
Table shows ciliate removal rates of zinc sulfate with different use concentrations
Calculating effective use concentration of zinc sulfate to obtain regression equation y (Probit) =7.1293+4.2184 log (D), and ciliate half-lethal concentration LD 50 =0.31278, an ld50 (Feiller correction) confidence limit of 95% = 0.26426-0.35823.
Table I shows that the use of zinc sulfate alone can kill ciliates in crab ponds, and the use concentration of the ciliates reaches more than 0.8mg/L to achieve 100 percent of killing effect, LD 50 0.31mg/L.
2. The synergistic effect of the chlorpyrifos (chlorpyrifos) with different proportions is calculated by adding the chlorpyrifos (the active ingredient of the chlorpyrifos) with the concentration of zinc sulfate (provided by the cleft) of 0.31mg/L as a fixed use dosage, and the synergistic effect is observed by adding the chlorpyrifos (the active ingredient of the chlorpyrifos) with different proportions, wherein the adding amount of the chlorpyrifos respectively accounts for 10%,20%,30%,40%,50% and 60% of the weight of the cleft.
Table two comparison of usage effects of trichloroisocyanuric acid added with different proportions
As can be seen from Table II, the concentration LD was used in zinc sulfate (supplied by KluyLess) 50 Under the condition of 0.31mg/L, along with the increase of the additive amount of chlorpyrifos (the effective component trichloroisocyanuric acid), the effect of the preparation on killing ciliates is also enhanced, and when the adding proportion of chlorpyrifos reaches more than 33.33%, the effect on killing ciliates reaches 100%. Considering that the price of trichloroisocyanuric acid is slightly higher than that of zinc sulfate, the use proportion of the fixed chlorpyrifos is 33.3-35%.
Test 2, different methods of use test materials were compared:
method of use # 1: 65% of fiber and 35% of chlorpyrifos. Diluting chlorpyrifos with 5 liters of water in a plastic barrel, uniformly stirring with a wood rod or a plastic rod, adding the fiber, continuously stirring, and uniformly mixing, wherein the pond dosage is 0.31mg/L (calculated by zinc sulfate).
Method of use # 2: the weight of the fiber is 65 percent and the weight of the chlorpyrifos is 35 percent, and the fiber is directly put into an aquarium with the dosage of 0.31mg/L (calculated by zinc sulfate).
Each group tested 2 aquariums, the test environment and conditions were the same as those tested one.
TABLE III effects of different methods of use
The test results are shown in the table, the removal rates of ciliates are different in the two using methods, and under the premise of fully and uniformly mixing, the test subject group considers that zinc sulfate (cleft) and trichloroisocyanuric acid (chlorpyrifos) have a better effect, and the trichloroisocyanuric acid (chlorpyrifos) can be rapidly dissolved in water, and the zinc sulfate is a crystalline substance, has a slow dissolution rate and poor effect, and when the zinc sulfate is completely dissolved in water, the oxidation reduction effect of the trichloroisocyanuric acid is reduced under the influence of other organic impurities in pond water. Therefore, the two are mixed uniformly and then sprayed, the trichloroisocyanuric acid has strong oxidation-reduction reaction, the water temperature in the barrel is high after the use, and the zinc sulfate is added after the trichloroisocyanuric acid is dissolved.
Test 3 influence of the formulation on aquatic weeds in a culture pond
The three waterweeds, namely the waterweed, the bitter grass and the black-leaf-black-algae, which are used for cultivating the eriocheir sinensis are moved into the same water cluster box together, temporarily cultivated for 3 days, and the test after the waterweed is observed, wherein the experimental conditions are as in the test 1. 65% of fiber and 35% of chlorpyrifos. Diluting chlorpyrifos with 5L of water in a plastic barrel, uniformly stirring with a wood rod or a plastic rod, adding fiber (zinc sulfate), continuously stirring, and uniformly mixing for later use. The water plants were observed after 5 days at concentrations of 0.31mg/L, 0.62mg/L, 0.93mg/L, 1.24mg/L, 1.5mg/L (calculated as zinc sulfate), respectively, and the results are shown in the following Table:
table four hazard of zinc sulfate of different concentrations to aquatic weeds
Experiments show that the dosage of 0.31mg/L, 0.62mg/L, 0.93mg/L and 1.2mg/L has no influence on the production of 3 aquatic weeds, the dosage of 1.5mg/L has influence on the growth of the waterweeds and the growth of the hydrilla verticillata and the hydrilla verticillata to cause the death of the aquatic weeds, but the preparation is safe for the aquatic weeds when the concentration of the preparation in actual production is 4 times that of the effective dosage (1.24 mg/L), so that we can obtain that: the product is safe to aquatic weeds under normal use dose.
Experiment 4, pond application paradigm 1:
the culture pond is positioned at the red star bridge town at the side of the Taihu lake, the pond is 10 mu, the average water depth is 50cm, the water grass is the kucao and the Haematococcus, 3.5 bags (500 g per bag, wherein 65 percent of fiber is obtained by gram and 35 percent of chlorpyrifos) are used for the preparation of the invention, the pond water is uniformly mixed according to the method, and then the whole pond is sprayed for the application of the preparation at 14:30 afternoon. Third day 8 am: 00, the eriocheir sinensis eats normally, ciliates are not seen in microscopic examination, and after one week, the growth of the Haematococcus and the herba sophorae flavescentis is observed normally.
Example 2:
the culture pond is positioned at the inner town of the Taihu lake side weave in Huzhou, the pond is 8 mu, the average water depth is 60 cm, the water grass is the kucao and the hydrilla verticillata, 3 bags (500 g of each bag, wherein 65 percent of fiber is obtained by gram and 35 percent of chlorpyrifos) of the preparation are used, the whole pond is sprayed after being uniformly mixed with pond water according to the method, and the medicine is applied at 9:30 am. Third day 9 am: 00, the eriocheir sinensis eats normally, ciliates are not seen in microscopic examination, and after one week, the growth of the kucao and the black algae on the leaves is observed to be normal.
The above-described embodiment is only a preferred embodiment of the present invention, and is not limited in any way, and other variations and modifications may be made without departing from the technical aspects set forth in the claims.
Claims (1)
1. The application of chlorpyrifos as a fibrinolytic synergist in preparing a preparation for preventing and controlling ciliates of eriocheir sinensis is characterized in that:
the preparation for preventing and controlling ciliates of eriocheir sinensis consists of fiber-killing agent and chlorpyrifos, wherein the weight percentage of the fiber-killing agent is 65%, the weight percentage of the chlorpyrifos is 35%, and the sum of the two is 100%; the zinc sulfate heptahydrate content in the fiber is 60%; the content of trichloroisocyanuric acid in the chlorpyrifos is 50%; the using method comprises the following steps: diluting chlorpyrifos with 5 liters of water in a plastic barrel, uniformly stirring with a wood rod or a plastic rod, putting fiber into the plastic barrel, continuously stirring, uniformly mixing, and calculating the using dosage of the pond to be 0.31mg/L in terms of zinc sulfate;
the chlorpyrifos can obviously improve the killing effect of the clever, thus the effect of killing ciliates can be improved under the condition of not increasing the dosage of the clever, thereby the dosage of the clever can be effectively reduced, and the chlorpyrifos does not hurt pasture and eriocheir sinensis, and can disinfect water.
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