CN114668020B

CN114668020B - Algae control composition

Info

Publication number: CN114668020B
Application number: CN202011556712.3A
Authority: CN
Inventors: 黄小威; 翁爱珍; 张有根; 杨婷婷; 陈润丽; 徐宗余; 陈俊鹏
Original assignee: Ningbo Sunjoy Agroscience Co Ltd
Current assignee: Ningbo Sunjoy Agroscience Co Ltd
Priority date: 2020-12-24
Filing date: 2020-12-24
Publication date: 2024-03-12
Anticipated expiration: 2040-12-24
Also published as: CN114668020A

Abstract

The invention discloses an algae control composition, which comprises water-soluble copper and a complex thereof and herbicide, wherein the weight ratio of the copper content in the water-soluble copper and the complex thereof to the herbicide is 0-99:99-0. According to the invention, the water-soluble copper and the complex thereof are matched with the herbicide for use, compared with the dosage of a single agent, after the dosage of 1/3 of the single ingredient is mixed in the mixed agent, the generated synergistic effect is better than 100% of the control effect of the single agent, the synergistic effect is realized, meanwhile, the quick acting property and the lasting period are well balanced, the control cost is greatly reduced, and the use cost is only 40-60% of the cost of the single agent.

Description

Algae control composition

Technical Field

The invention belongs to the technical field of pesticides, and particularly relates to an algae control composition for places such as rivers and lakes, paddy fields, ponds, reservoirs, rivers, landscape architecture, fish tanks, emulsion, paint and the like.

Background

In recent years, with the increase of eutrophication of water bodies, the frequency of blue algae bloom outbreaks in China and even worldwide is gradually increased, and the bloom outbreaks of blue algae and the like lead to the bloom outbreaks of ponds and rivers, the ponds are smelly, dead fishes and shrimps, particularly in Taihu and other places, the annual prevention and control pressure is high, and environmental protection departments also carry out algae control in water conservancy facility transformation, lake water quality control, reed water grass planting in lakes and the like, and prevent in advance. However, when the meteorological water quality condition is proper, blue algae and filiform algae are easy to burst, the coverage is wide, the situation is rapid, and the control cannot be realized.

In addition, moss is very common and difficult to control in aquaculture, particularly in the production of crab and shrimp in recent years, and is the most headache management and cultivation problem for farmers; in rice planting, especially northeast rice planting, because of large phosphate fertilizer, moss burst is promoted to form water meter moss layer coverage, so that the surface area temperature of a paddy field is reduced, and the growth of rice is affected. Moss and rice are simultaneously competing for fertilizer and oxygen and competing for resources, aiming at the prevention and control of algae, the prior art mainly focuses on opacifiers such as copper-based algicides, peroxides, quaternary ammonium salts, phthalocyanine copper and the like, and trisazo benzene herbicides.

The water-soluble copper and the complex thereof release copper ions or chelate copper ions to kill algae, and have two action mechanisms: firstly, the photosynthesis progress of algae can be interrupted, so that the algae die; secondly, the protease in the algae cells can be denatured, and the cells are inactivated and dead. The two mechanisms act together to make the copper-based algicide take effect quickly. The water-soluble copper and its complex are characterized by good quick-acting property, but short duration. If the water environment is required to be kept in a healthy state for a long time, high-frequency application is required, and labor cost and material cost are high. Excessive application of copper formulations can also cause copper ion accumulation, causing environmental concerns.

The herbicide is used for controlling algae, the herbicide algicides with the largest dosage on the market at present are prometryn and diuron, the prometryn and diuron have low cost and high cost performance, but the half lives of the prometryn and diuron are very long, and the whole system is provided with the herbicide algicidesHalf-life DT of prometryn ₅₀ Up to 110-236 days; diuron half life DT ₅₀ Also for 90-180 days. In practical production practice, users like the perception of good quick-acting property, and the algae floating on the surface of the water surface are killed, and the algae are often used according to the highest dosage, even multiple dosage. As a result of excessive use, a large amount of submerged plants such as the waterweed and the like in the water body die due to long residues, and the death accident of fish and crab culture often occurs, so that serious economic loss is brought to the farmers.

Other products for killing algae, such as quaternary ammonium salts like polythiophene ammonium chloride, are widely used for killing algae in aspects of swimming pools, pipeline construction, daily chemicals and the like, but are highly toxic to aquatic organisms, especially crustaceans like shrimp and crab, and can not be used in water sources like ponds and reservoirs; the peroxide has good blue algae effect and is environment-friendly, but has low activity and high use cost, and can be only used in places with low price; the phthalocyanine copper is an excellent light-shading agent, is particularly suitable for water bodies with deeper water depth, but has relatively poor effect and more application scene limitation for environments with shallow water bodies or large-area expansion of algae, so that the phthalocyanine copper is developed for sites such as natural water bodies, bonsais and aquatic production, can control excessive propagation of algae, and is very necessary for products with good compatibility to water environment.

Disclosure of Invention

Aiming at the problems existing in the prior art, the invention aims to provide an algae control composition which can control excessive propagation of planktonic algae such as blue algae and filamentous algae such as spirogyra and can avoid water pollution accidents such as large-area blue algae bloom and moss burst and can also be used for pollution control of harmful algae on the surfaces of articles.

The invention is realized by the following technical scheme:

the algae control composition is characterized by comprising two active components, namely a component A and a component B, wherein the component A is water-soluble copper and a complex thereof, the component B is herbicide, and the weight ratio of the component A to the component B is 0-99:99-0.

Preferably, the weight ratio of the copper content in the water-soluble copper and the complex thereof to the herbicide is 0-50:60-0.

Preferably, the weight ratio of the copper content in the water-soluble copper and the complex thereof to the herbicide is 0.5-20:50-1.

Preferably, the water-soluble copper and the complex thereof comprise any one or more of copper chloride, copper sulfate, copper nitrate, copper citrate, copper gluconate, copper ammine, copper acetate, copper ethanolamine, copper triethanolamine, copper ethylenediamine dihydroxide, copper ethylenediamine, copper rosinate and copper amino acid. These water-soluble copper and its complexes are commonly characterized as algicidal by releasing or chelating copper ions. The effective concentration of copper ions in the water body is 0.1-1.0ppm, and the concentration is increased along with the increase of the hardness of the water body.

Preferably, the herbicide is any one or a mixture of more than one of substituted urea herbicide, dinitroaniline herbicide and triazine herbicide, and the herbicide is a mixture of the same type of herbicide or a mixture of different types of herbicide. The three herbicides have the same characteristics in field efficacy experiments: the activity of mature algae is low, the mature algae can be killed by high-concentration dosage treatment, but the spore activity of the mature algae is high, and the mature algae can be shoveled out with extremely low dosage. The low temperature and low illumination have little influence on the effect.

Preferably, the substituted urea herbicide is any one of isoproturon, chlormeuron, diuron, fluometuron, chlormeuron and linuron.

Preferably, the dinitroaniline herbicide is any one of pendimethalin, butralin and trifluralin. The dinitroaniline herbicide has inhibiting effect on filamentous algae such as water net algae and aphanidermatum algae. Can inhibit the mitosis of algae cells, reduce the reproduction function of algae cells, is ineffective for mature algae, but can inhibit the formation of new spores. The fields are as follows: after the use, the floating algae remains green, and after the mature algae dies, no fresh algae community appears.

Preferably, the triazine herbicide is any one of prometryn, cilazathioprine, atrazine and metribuzin.

It is found that although the chemical structure classifications of two components of prometryn and diuron are different, one belongs to the class of sym-triazenes and the other belongs to the class of substituted ureas, the action mechanisms of the prometryn and the diuron are the same, and the difference of the activity of algae in different growth stages is large.

Taking the water reticulata in the filamentous algae as an example, the concentration of the prometryn for killing mature water reticulata to lose green is 1.5-2.0ppm. But when the concentration is 0.015-0.02ppm, the algae of the water net algae is green, but the growth is stopped, the biomass is not increased any more, the germination inhibiting concentration of the algae spores of the water net algae is 0.015-0.02ppm, and the activity difference of the algae spores of the water net algae is 100 times.

Diuron is used for treating the water screen algae, the symptoms are the same as those of prometryne, the activity of the diuron is lower than that of prometryne, the inhibition concentration of the diuron spores is 0.05-0.1ppm, but the death and green-losing concentration of the diuron is 2-5ppm, and the difference between the diuron and the prometryne is larger.

Different herbicide varieties are mixed and used, so that special effects can be brought. For example: the variety with good quick-acting property and the variety with high activity and long lasting period can be mixed for use, so that the overall quick-acting property can be increased, and the residual risk of the long-residual variety can be reduced. Such as isoproturon with short half-life (half-life DT) ₅₀ 6-28 days) and prometryn with long half-life (half-life DT ₅₀ 110-236 days), the amount of prometryn can be greatly reduced, the influence of prometryn residue on aquatic plants is reduced, and an ideal duration is maintained.

Preferably, the algae-controlling composition can be processed into various solid or liquid dosage forms, which are convenient to use, including but not limited to suspending agents, water dispersible granules, wettable powders, granules, soluble solutions, emulsifiable concentrates, microemulsions, oil suspensions, suspoemulsions, and microcapsule suspensions. Can treat the surface of water body and the inside of water body by means of broadcasting, water surface spraying, underwater pipe application and the like, and prevent or control the generation and development of algae.

The algae control composition is applied to controlling excessive propagation of various algae in river and lake, paddy field, pond, reservoir, river, landscape, fish tank, latex, paint place and materials.

The basis for the mixing of water-soluble copper and its complex with herbicide is as follows: herbicides such as isoproturon control algae, cut off energy sources for inhibiting photosynthesis of algae, and inhibit photoautotrophic growth process of algae; the activity of the composition on mature filamentous algae is low, the effect is slow, but the proliferation of the filamentous algae can be inhibited, the effect is slow, after the composition is taken, the obvious prevention effect can be seen only by 3 to 5 days, but the lasting period is long; the water-soluble copper and the complex thereof have quick response, and can kill most harmful algae after 3 hours of contact, but have short duration, and the medicament is required to be applied after 3-5 days. The biological activity of the water-soluble copper and the complex thereof is greatly influenced by illumination and temperature, and the low illumination and low temperature can greatly reduce the algae control activity, and even when the water temperature is lower than 15 ℃, the water is basically ineffective. The two components are mixed to exert their advantages and reduce the consumption of the two components at the same time, thus achieving the purpose of reducing and enhancing the efficiency.

According to the invention, the water-soluble copper and the complex thereof are matched with the herbicide for use, compared with the dosage of a single agent, after the dosage of 1/3 of the single ingredient is mixed in the mixed agent, the generated synergistic effect is better than 100% of the control effect of the single agent, the synergistic effect is realized, meanwhile, the quick acting property and the lasting period are well balanced, the control cost is greatly reduced, and the use cost is only 40-60% of the cost of the single agent.

Drawings

FIG. 1 shows moss growth after 7d treatment with different herbicides of test example 1;

fig. 2 is the moss microstructure of fig. 1, note: x10, is observed under a microscope at 10 times magnification; x40, is observed under a 40-fold microscope;

FIG. 3 is a graph showing comparison of CK and E50 after 5 days of administration in test example 2;

FIG. 4 is a graph showing comparison of CK and E75 after 5 days of administration in test example 2;

FIG. 5 is a graph comparing T200 and T150 after 5 days of administration in test example 2;

FIG. 6 is a graph comparing T200 and E150 after 5 days of administration in test example 2;

fig. 7 to 9 are diagrams showing comparison of the fresh water control region of test example 3 before and after administration (left side before administration, right side after administration);

FIGS. 10-12 are graphs comparing test example 3 before and after administration of 0.8ppm sweet spot treatment (left graph before administration, right graph after administration);

FIGS. 13-15 are graphs comparing treatment areas of 0.3ppm of sweet clear + 0.3ppm of isoproturon from test example 3 before and after administration (left graph before administration, right graph after administration);

FIG. 16 is an apparent representation of microcystis aeruginosa growth in test example 4 control treatments 0-168 h;

FIG. 17 is a graph showing the apparent growth of microcystis aeruginosa treated with 3ppm prometryn of test example 4 for 0-168 hours;

FIG. 18 is an apparent representation of the growth of microcystis aeruginosa from 0 to 168 hours of 3ppm treatment with isoproturon in test example 4;

FIG. 19 is an apparent representation of the growth of microcystis aeruginosa from 0 to 168 hours of 3ppm treatment of chlortoluron in test example 4;

FIG. 20 is a graph showing the apparent growth of microcystis aeruginosa treated with 0.3ppm atrazine for 0-168 hours in test example 4;

FIG. 21 shows the change in OD value of microcystis aeruginosa in test example 4 for each treatment of 0-168 hours.

Detailed Description

The invention will be described in further detail with reference to specific examples below in order to provide a better understanding of the invention.

Formulation example 1:400g/L of copper sulfate pentahydrate isoproturon soluble liquid, wherein the content of copper sulfate pentahydrate is 300g/L, the content of isoproturon is 100g/L, the content of dimethylformamide cosolvent is 10%, the content of Tween 80 is 3%, the content of agricultural emulsion is 600, and the water is 100%.

Formulation example 2: the water dispersible granule comprises 60% of anhydrous copper sulfate, long Hanliang% of green wheat, 1.5% of sodium lauryl sulfate, 1.5% of Terwet 1004, 2% of sodium carboxymethyl starch, 2% of Morwet D425 and 100% of kaolin.

Formulation example 3:70% of triethanolamine copper-pendimethalin wettable powder, wherein the content of the triethanolamine copper is 55%, the content of the pendimethalin is 15%, the content of the pesticide emulsion OX-656 is 2%, the content of the MF dispersing agent is 4%, and the talcum powder is 100%.

Formulation example 4:40% of copper acetate-prometryn emulsifiable concentrate, wherein the content of copper acetate is 30%, the content of prometryn is 10%, the content of alkylphenol formaldehyde resin polyoxyethylene ether is 3%, the content of sucrose glyceride is 2%, and the content of naphtha is 100%.

Formulation example 5: the water dispersible granule comprises copper gluconate and diuron 80%, wherein the content of the copper gluconate is 70%, dioctyl sodium sulfosuccinate is 2%, sodium glycocholate is 1%, methylene dinaphthyl sulfonate is 1%, and anhydrous sodium sulfate is 100%.

Formulation example 6:40% of a copper-atrazine suspending agent, wherein 30% of copper-atrazine, 10% of atrazine, 2% of styryl phenol polyoxyethylene ether, 1% of agricultural emulsion 601# content, 3% of epichlorohydrin, 1% of sodium carboxymethyl cellulose, 4% of propylene glycol, 0.1% of tributyl phosphate and 100% of water are added.

Formulation example 7:1.5% of copper chloride and simetryn granules, wherein 1% of copper chloride, 0.5% of simetryn, 20% of adsorbent diatomite, 1% of dispersant fatty alcohol polyoxyethylene ether, 2% of binder bentonite, 0.1% of warning color acid scarlet and 100% of attapulgite as a carrier.

Formulation example 8: the microcapsule suspending agent comprises copper citrate of 25%, oxaziclomefone of which the content is 22%, oxaziclomefone of which the content is 3%, polyurea encapsulating agent of which the content is 3%, solvent oil of which the content is 150# of a capsule core solvent is 5%, emulsifier agricultural emulsion OX-656 2%, stabilizer epichlorohydrin of 0.5%, dispersing agent methylene dinaphthyl sulfonate (NPF) of 2%, xanthan gum XG of 0.5%, antifreeze propylene glycol of 3%, defoamer organosilicon emulsion of which the content is 100% and water of which the content is 100%, and the interfacial polymerization method.

Test example 1: indoor pesticide effect screening of herbicide on moss

On the basis of the earlier study, the algae killing effect of the mixed use of pendimethalin and copper sulfate is studied, and the moss prevention effect of oxadiazon, oxyfluorfen, diuron, bromoxynil octanoate, clomazone, oxazinone and the compounding of a plurality of medicaments is explored.

Test method

Putting 0.25g of cleaned and rotated aphanidermatum into a plastic cup, adding 150mL of tap water, and adapting to culture for 3d; preparing 1000ppm stock solution of each herbicide, and proportionally adding oxadiazon, oxyfluorfen, diuron, bromoxynil octanoate, clomazone, oxazinone, pendimethalin, 0.50ppm of sweet clear, 1.00ppm and sweet clear: pendimethalin is compounded into the components of 0.5:0.25ppm, 0.5:0.5ppm, 0.5:1.0ppm and 1.0:10ppm, bromoxynil octanoate: the proportion of pendimethalin is 0.5:0.5ppm, 1.0:1.0ppm, 2 replicates per treatment, and equal amounts of fresh water were added as controls. Culturing at 25-30deg.C for 12 hr at a light-dark time ratio of 12 hr. The effect of the agent on moss control was assessed by visual inspection as shown in figure 1.

The copper sulfate sample used in the test is 410g/L of pentahydrate copper sulfate aqueous solution, the copper content is 10g/L, and the trademark isThe manufacturer is Sanjiang Yinong chemical Co., ltd.

The test results are shown in Table 1, and the structure shows that:

1) This test shows that copper ions are also the fastest in terms of the quick-acting nature of moss yellowing;

2) The low-dose pendimethalin has no obvious effect on the aged algae, but the algae is contracted after the medicine is taken, and the pendimethalin possibly has an inhibiting effect on the newly grown algae;

3) After the pendimethalin and the copper sulfate are mixed, the integral yellowing speed is consistent with that of the single copper sulfate, but the algae control effect is better than that of the single copper sulfate and the single pendimethalin, and the synergistic effect is achieved. In particular, the effect of the two low-dose combined treatments is better than that of the high-dose single-dose treatment;

4) Diuron has obvious inhibition on the photosynthesis of the aphanidermatum (bubbles are not generated after the aphanidermatum is taken), and moss can be settled in one day, but yellowing is not as sweet as that of the aphanidermatum; the oxyfluorfen has an inhibition effect on mature algae, can also inhibit moss growth and reduce bubbles;

5) The rest medicaments have no obvious control effect on moss at the test concentration.

TABLE 1 changes in the form of 1-7d changes in the form of aphanidermatum after treatment with each agent

Test example 2: field efficacy test of crab paddy field spirogyra control agent

In order to know the action mechanism of the copper preparation and isopropyl Long Shanji on moss, and observe the mixed effect of the copper preparation and isopropyl Long Shanji, and simultaneously observe the influence on crab growth. The water environment protection research project group of Ningbo Sanjiang Yinong chemical Co., ltd in 2020 performs field efficacy verification tests on eight farms in Cao Feidian area of Tangshan city, hebei province.

Test crops and varieties: rice-Jijin Yuan 89 (transplanting in 25 days 5 months, mechanical transplanting, rice row spacing 16 cm); river crab-Eriocheir sinensis (140 per kg of river crabs are bred, and 700 per mu of density are bred).

Test time: 4/2020, 6/9

Control object: spirogyra

Test agent: 410g/L aqueous solution of copper sulfate pentahydrate (trade mark of Ningbo Sanjiang Yinong chemical Co., ltd.)) The method comprises the steps of carrying out a first treatment on the surface of the 50% isoproturon wettable powder (manufactured by Jiangsu Kuida Co.); 60% copper sulfate pentahydrate 20% isoproturon wettable powder (Cu: isoproturon=15:20, ningbo Sanjiang Yinong chemical Co., ltd.).

Soil type: black loam.

And (3) test design: the test adopts a district test, the ridge is built between test fields to be separated, 11 treatments are arranged, the repeated operation is performed for 3 times, the total number of the districts is 33, the area of each district is 25 square meters, and the districts are arranged randomly.

Treatment 1:50 ml/mu (T50-1, T50-2, T50-3);

treatment 2:75 ml/mu (T75-1, T75-2, T75-3);

treatment 3:100 ml/mu (T100-1, T100-2, T100-3);

treatment 4:150 ml/mu (T150-1, T150-2, T150-3);

treatment 5:200 ml/mu (T200-1, T200-2, T200-3);

treatment 6: isoproturon 50 g/mu (E50-1, E50-2, E50-3);

treatment 7: isoproturon 75 g/mu (E75-1, E75-2, E75-3);

treatment 8: isoproturon 100 g/mu (E100-1, E100-2, E100-3);

treatment 9: isoproturon 150 g/mu (E150-1, E150-2, E150-3);

treatment 10:60% copper sulfate pentahydrate 20% isoproturon wettable powder 45 g/mu (Z-1, Z-2, Z-3);

treatment 11: blank (without any medication applied).

The application method comprises the following steps: the medicine is applied 1 time in 6 th and 4 th of 2020, and is sprayed by a 5L manual sprayer, and the spraying amount is 1L/25m ² 。

The investigation method comprises the following steps: investigation is carried out 3 times 1, 3 and 5 days after the application, the control effect is visually examined according to the coverage of the cotton in the district, the stored cotton is fished up by a five-point sampling method (20 cm per point area is 30 cm) 3 days after the application, and the fresh weight control effect is calculated by weighing. The phytotoxicity of each treatment to rice was observed 1, 3 and 5 days after the application.

Fresh weight control (%) = (control zone biomass-treatment zone biomass)/control zone biomass x 100%.

Test results:

the control effect of the three agents on the spirogyra in the crab paddy fields is shown in Table 2, and the individual comparison diagrams are shown in figures 3-6.

Table 2 Effect of three agents on control of a crab paddy floss

As shown by the test results in the table 1, the 80% copper sulfate pentahydrate isoproturon wettable powder has the best control effect on the water cotton of the crab paddy field, the quick acting property is better than that of a single dosage of 50% isoproturon wettable powder, and the single dosage is consistent with each treatment of 410g/L copper sulfate pentahydrate aqueous solution, but the final lasting period is consistent with the high dosage of 50% isoproturon wettable powder. From the coverage control effect of 3 days after the medicine, the control effect of 50% isoproturon wettable powder on the cotton is over 90% and the difference between the concentrations is not obvious; the control effect of 410g/L aqueous solution of copper sulfate pentahydrate on the spirogyra increases with the increase of concentration. The fresh weight prevention effect after 3 days of the medicine is above 60%, and the fresh weight prevention effect comprises 150 ml/mu and 200 ml/mu of 410 g/liter of aqueous solution of copper sulfate pentahydrate and all treatments of 50% of wettable powder, wherein the prevention effect of 150 ml/mu of the aqueous solution of copper sulfate pentahydrate is optimally 70.48%, and the prevention effect of 150 ml/mu of 50% of wettable powder is optimally 91.12%.

From the experimental results, the two are mixed to have obvious synergistic effect, and the small dosage of the mixed agent can obtain the prevention effect which can be achieved by single copper sulfate or isopropyl Long Shanji high-dose treatment.

Rice phytotoxicity and crab safety investigation result: no adverse effect of the test agent on the growth and development of rice is seen in the test period, and the test agent is safe and harmless to rice. No effect on crabs was observed.

The test results of the test example show that the three medicaments have certain control effect on the spirogyra, wherein the 80% copper sulfate pentahydrate isoproturon wettable powder has the best overall control effect, and the lower dosage shows better synergistic effect. In the process of investigation and test results, the isoproturon has good control effect on the newly grown spirogyra at the bottom layer, so that the fresh weight control effect is better than that of a 410g/L aqueous solution of cupric sulfate pentahydrate. The control effect of the 410g/L aqueous solution of copper sulfate pentahydrate in 150 ml/mu is inferior to the former two, and the control effect of the 410g/L aqueous solution of copper sulfate pentahydrate on the surface floss is found to be superior to that of the bottom layer newly grown floss in the investigation test result process, so that the effect can be seen after 1 day of application, and the coverage control effect is superior to that of 50% isoproturon wettable powder after 1 day of application, but the overall control effect is poor. In the prevention and control period, the application of the pesticide in the early stage of the occurrence of the spirogyra is recommended, and the prevention and control effect is better.

Test example 3: autumn and winter formula algicide field test

The purpose is as follows: and verifying the field efficacy of the formula medicament through field efficacy tests.

Test time, place, conditions: 11 months 15-11 months 18 days in 2020, hubei Zhijiang Longdan bay town Li Gutai, air temperature 20 ℃, average width of the annular groove of 4m and depth of 0.5m.

Test materials

Test target: moss bloom in a cultivation water area caused by spirogyra, aphanidermatum, and the like;

test agent: sweet clear (410 g/L copper sulfate pentahydrate aqua), sweet clear+isoproturon;

experimental instrument: 100ml measuring cylinder, small electronic balance, measuring tape (50 meters), meter ruler (5 meters), electric sprayer.

The test method comprises the following steps: the moss coverage of the test points exceeds 50%, the chemical is prepared after the water quantity of the circular ditch water body is calculated, and 0.8ppm of sweet clear and 0.3ppm of sweet clear plus 0.3ppm of isoproturon are respectively applied to two opposite circular ditch water bodies by adopting a spray application mode; equal amounts of clear water were applied to the lateral annular grooves as a control treatment. The test records are shown in Table 3.

Investigation of results: 3 observation points are selected for each treated annular ditch, and the algae control effect of the agent is evaluated by observing moss coverage (including underwater and water surface).

The coverage control formula is as follows:

wherein:

e, inhibition rate, the unit is;

d0—average moss coverage before treatment;

dt—average coverage of moss after treatment.

Test results: compared with a clear water control area, the sweet clear water 0.8ppm has very obvious control effect, and the control effect reaches 92.1 percent; the control effect of 0.3ppm of sweet clear and 0.3ppm of isoproturon is obviously better than that of the treatment of a sweet inventory agent, and the control effect reaches 99.2 percent; the control effect on floating moss and underwater moss is very thorough.

Table 3 test record

Test example 4: test for controlling effect and duration of isoproturon, chlormeuron, prometryn and atrazine on microcystis aeruginosa

The purpose is as follows: through experiments, the control effect and the control duration difference of isoproturon, chlormeuron, prometryn and atrazine on microcystis aeruginosa are clear.

Time: 10/22/2019-10/22/2020

Test materials: test target: microcystis aeruginosa (FACHB-911) is purchased from the algae species library of the national academy of sciences and cultured in the BG-11 medium at room temperature (25+ -1deg.C) with a photoperiod of 12h: shake flask culture for 12h, shake 2 times per day.

Test agent: 50% isoproturon wettable powder, 25% chlormeuron wettable powder, 40% prometryn wettable powder and 38% atrazine suspending agent.

The test method comprises the following steps: transferring 300mL of well-grown algae liquid into 500mL of sterilized BG-11 culture medium under aseptic condition, shake-flask culturing at 25+ -1deg.C under indoor 12h:12h illumination for 1 day, and packaging into 100mL triangular flasks with 50mL algae liquid per flask. Adding the medicament under aseptic conditions: isoproturon, chlortoluron, prometryne and atrazine 0.3ppm; 3 replicates per treatment, shaking culture was continued (small amounts of culture broth were periodically added to the system). The OD values (OD values correspond to the number density of algal cells, and the number of algal cells increases as the OD values increase) of each treatment were measured under aseptic conditions at 1, 15, 30, 60, 120, 240, and 360d, respectively, and the relative inhibition rate against microcystis aeruginosa was calculated.

Ir= (control OD value-treatment OD value)/control OD value

Test results: OD values of microcystis aeruginosa growth for each treatment of 0-360d are shown in Table 4; the inhibition rate of each treatment for 0-168 hours on the growth of microcystis aeruginosa is shown in table 5; the OD value of microcystis aeruginosa was varied from 0-168h for each treatment as shown in FIG. 21.

Four herbicides show good inhibition effect on microcystis aeruginosa in the early stage (15 d), and the number of algae cells can be controlled at a low level; the isoproturon is controlled for 15-30d, and the algae cell number is recovered to the control level after 30 d; the control time of the chlortoluron is about 60-120d, and the algae cell number is recovered to the control level after 120 d; the control time of prometryn and atrazine is over 240d; the apparent growth appearance of microcystis aeruginosa is shown in figures 16-20.

TABLE 4 OD values for microcystis aeruginosa growth for treatments 0-360d (the greater the OD value, the greater the number of algal cells)

TABLE 5 inhibition of microcystis aeruginosa growth by each treatment for 0-168h

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Claims

1. The algae-controlling composition is characterized by comprising two active components, namely a component A and a component B, wherein the component A is copper sulfate pentahydrate, the component B is isoproturon, and the weight ratio of the component A to the component B is 3:1.

2. The algae control composition of claim 1, wherein the algae control composition is formulated as any one of a suspension, a water dispersible granule, a wettable powder, a granule, a soluble liquid, an emulsifiable concentrate, a microemulsion, an oil suspension, a suspoemulsion, and a microcapsule suspension.

3. Use of an algae-controlling composition according to any one of claims 1-2 for controlling excessive proliferation of algae in rivers, lakes, paddy fields, ponds, reservoirs, landscapes, fish ponds and paint sites.