CN114668020A

CN114668020A - Algae control composition

Info

Publication number: CN114668020A
Application number: CN202011556712.3A
Authority: CN
Inventors: 黄小威; 翁爱珍; 张有根; 杨婷婷; 陈润丽; 徐宗余; 陈俊鹏
Original assignee: Ningbo Sunjoy Agroscience Co Ltd
Current assignee: Ningbo Sunjoy Agroscience Co Ltd
Priority date: 2020-12-24
Filing date: 2020-12-24
Publication date: 2022-06-28
Anticipated expiration: 2040-12-24
Also published as: CN114668020B

Abstract

The invention discloses an algae control composition, which comprises water-soluble copper and a complex thereof and a herbicide, wherein the weight ratio of the content of the copper in the water-soluble copper and the complex thereof to the herbicide is 0-99: 99-0. According to the invention, the water-soluble copper and the complex thereof are used in combination with the herbicide, compared with the dosage of a single agent, the dosage of the single component 1/3 in the mixed agent is mixed, the generated synergistic effect is superior to the 100% prevention effect of the single agent, the synergistic effect is realized, simultaneously the quick action and the lasting period are well balanced, the prevention and control cost is greatly reduced, and the use cost is only 40-60% of the cost of the single agent.

Description

Algae control composition

Technical Field

The invention belongs to the technical field of pesticides, and particularly relates to an algae control composition for places such as rivers, lakes, rice fields, ponds, reservoirs, rivers, landscape landscapes, fish tanks, fish ponds, latex, paint and the like.

Background

In recent years, along with the aggravation of water eutrophication, the frequency of cyanobacterial bloom outbreak in China and even in the world is gradually increased, cyanobacterial bloom outbreaks cause pond and river bloom outbreaks, ponds are smelly, dead fishes and shrimps die, particularly Taihu lake and other places, the annual prevention and control pressure is higher, and the environmental protection department also carries out algae control in measures such as water conservancy facility modification, lake-entering water quality control, reed and aquatic weed planting along the lake and the like so as to prevent in advance. However, when the meteorological water quality conditions are appropriate, blue algae and filamentous algae are easy to burst, the coverage area is wide, and the coming trend is rapid and cannot be controlled.

In addition, in aquaculture, especially in the production of crab and shrimp culture in recent years, moss is very common and very difficult to control, and is the most troublesome management and cultivation problem for farmers; in rice planting, especially in northeast rice planting, due to the fact that the phosphate fertilizer is used greatly, moss is promoted to burst, water moss layer coverage is formed, accumulated temperature on the surface of a rice field is reduced, and rice growth is affected. The moss and rice compete for oxygen and resources, and aiming at prevention and control of algae, the moss mainly focuses on copper-based algae control agents, peroxides, quaternary ammonium salts, copper phthalocyanine and other opacifiers and triazine herbicides at present.

The water-soluble copper and the complex thereof release copper ions or chelate copper ions to kill algae, and have two action mechanisms: firstly, the photosynthesis process of algae can be interrupted, so that the algae die; secondly, the protease in the algae cell can be denatured, and the cell is inactivated and killed. The two mechanisms act together to ensure that the copper-based algicide has quick response. The water-soluble copper and its complex compound features quick acting, but short effective period. If the water environment needs to be kept in a healthy state for a long time, high-frequency application is needed, and the labor cost and the material cost are high. Excessive application of copper formulations can also cause copper ion accumulation, which can cause environmental problems.

The herbicide is adopted for controlling algae, the herbicide algicide with the largest use amount in the current market is prometryn and diuron, the cost of the prometryn and diuron is low, the cost performance is high, but the half-life period of the prometryn and diuron is very long, and in the whole system, the half-life period DT of the prometryn is₅₀Up to 110-; diuron half-life DT₅₀The treatment period is also 90-180 days. In actual production practice, users prefer the sense of good quick-acting property, and the algae floating on the surface of the water surface are killed by using the highest dose or even multiple doses. As a result of excessive use, a large number of large submerged plants such as elodea in the water body are dead due to long residues, death accidents of fish and crabs are frequently caused, and serious economic loss is brought to farmers.

Other products for killing algae, such as quaternary ammonium salts such as polythia ammonium chloride and the like are widely used for killing algae in swimming pools, pipeline construction, daily chemicals and other aspects, but the products are highly toxic to aquatic organisms, particularly crustaceans such as shrimps and crabs and the like and cannot be used in ponds, reservoirs and other water sources; the peroxide has good effect on blue algae and is environment-friendly, but has low activity and high use cost, and can only be used in places which are not sensitive to price; phthalocyanine copper is an excellent opacifier, is particularly suitable for water bodies with deep water depth, but has poor effect on the environment that the water bodies are shallow or algae are expanded in a large area, and has more application scene limitations, so that the development of a product which can be used in places such as natural water bodies, bonsais, aquatic product production and the like, can control the excessive propagation of the algae and has good water environment compatibility is very necessary.

Disclosure of Invention

Aiming at the problems in the prior art, the invention aims to provide an algae control composition which can control excessive propagation of floating algae such as blue-green algae and filamentous algae such as spirogyra, avoid water body pollution accidents such as large-area cyanobacterial bloom and moss outbreak and can also be used for controlling pollution of harmful algae on the surface of articles.

The invention is realized by the following technical scheme:

the algae control composition is characterized by comprising two active components, namely a component A and a component B, wherein the component A is water-soluble copper and a complex thereof, the component B is a herbicide, and the weight part ratio of the component A to the component B is 0-99: 99-0.

Preferably, the weight ratio of the copper content in the water-soluble copper and the complex thereof to the herbicide is 0-50: 60-0.

Preferably, the weight ratio of the content of the copper in the water-soluble copper and the complex thereof to the herbicide is 0.5-20: 50-1.

Preferably, the water-soluble copper and the complex thereof comprise any one or a mixture of more than one of copper chloride, copper sulfate, copper nitrate, copper citrate, copper gluconate, copper ammine, copper acetate, copper ethanolamine, copper triethanolamine, copper ethylenediamine double hydroxide, copper ethylenediamine, copper rosinate and copper amino acid. A common feature of these water-soluble copper and its complexes is that they kill algae by releasing or chelating copper ions. The effective concentration of copper ions in the water body is 0.1-1.0ppm, and the concentration is increased along with the increase of the water hardness.

Preferably, the herbicide is any one of or a mixture of more than one of substituted urea herbicides, dinitroaniline herbicides and triazine herbicides, and the herbicide is a mixture of the same type of herbicides or a mixture of different types of herbicides. In the field efficacy test, the three herbicides have the same characteristics: the activity to mature algae is low, and the mature algae can be killed only by high-concentration dosage treatment, but the activity to spores is high, and the spores can be eradicated by extremely low dosage. The effect is little influenced by low temperature and low illumination.

Preferably, the substituted urea herbicide is any one of isoproturon, chlortoluron, diuron, fluometuron, glusulfuron, prosulfuron and linuron.

Preferably, the dinitroaniline herbicide is any one of pendimethalin, butralin and trifluralin. The dinitroaniline herbicide has inhibiting effect on filamentous algae such as Neurospora aquatica and Tetranychus urticae. Can inhibit the mitosis of algae cells and reduce the reproductive function of algae cells, which is reflected in that the algae cells are ineffective for mature algae but can inhibit the formation of new spores. The field performance is as follows: after the floating algae is used, the floating algae keeps green, and after the mature algae die, a fresh algae community does not appear any more.

Preferably, the triazine herbicide is any one of prometryn, simetryn, atrazine and metribuzin.

The research finds that although the chemical structure classification of two components of prometryn and diuron is different, one belongs to sym-triazine and the other belongs to substituted urea, the two components have the same action mechanism, are both photosynthesis inhibitors and have large activity difference on algae in different growth stages.

Taking the dictyophytes in filamentous algae as an example, the lethal green-losing concentration of prometryn on mature dictyophytes is 1.5-2.0 ppm. But at the concentration of 0.015-0.02ppm, the dictyococcus zornians keeps green, but stops growing, the biomass is not increased any more, the germination inhibiting concentration of the dictyococcus zornians spores is 0.015-0.02ppm, and the activity difference of the two reaches 100 times.

The diuron is used for treating the dictyota, the symptoms are the same as those of prometryn, the activity of the diuron to the dictyota is lower than that of the prometryn, the concentration of inhibiting spores of the dictyota is 0.05-0.1ppm, but the concentration of killing and green-losing to mature dictyota is 2-5ppm, and the front and back difference is large.

The mixture of different herbicides can bring special effect. For example: the variety with good quick-acting property and the variety with high activity but long lasting period are mixed, so that the whole quick-acting property can be increased, and the residual risk of the long-residual variety can be reduced. Such as isoproturon with short half-life (half-life DT) ₅₀6-28 days) and long half-life prometryn (half-life DT)₅₀110-236 days), the usage amount of prometryn can be greatly reduced,reduces the influence of prometryn residue on aquatic plants, but maintains an ideal lasting period.

Preferably, the algae control composition can be processed into various solid or liquid dosage forms, which are convenient to use and include but not limited to suspending agents, water dispersible granules, wettable powder, granules, soluble liquid agents, missible oil, microemulsion, oil suspending agents, suspoemulsion and microcapsule suspending agents. The method can be used for treating the surface and the interior of the water body by means of broadcasting, water surface spraying, water bottom pipe application and the like, and preventing or controlling the generation and development of algae.

The algae-controlling composition is applied to controlling excessive propagation of various algae in rivers, lakes, paddy fields, ponds, reservoirs, rivers, garden landscapes, fish tanks, fish ponds, latex and paint places and materials.

The water-soluble copper and the complex thereof are mixed with the herbicide according to the following formula: the herbicide such as isoproturon and the like controls algae, cuts off energy sources for inhibiting the photosynthesis of the algae and inhibits the photoautotrophic growth process of the algae; the activity of the compound preparation on mature filamentous algae is low, the effect is slow, but the proliferation of the filamentous algae can be inhibited, the effect is slow, after the compound preparation is taken, the obvious prevention effect can be seen in 3-5 days, but the effect duration is long; the water-soluble copper and the complex thereof have quick response, most harmful algae can be killed after the contact for 3 hours, but the lasting period is short, and the supplement is needed after 3 to 5 days. The biological activity of the water-soluble copper and the complex thereof is greatly influenced by illumination and temperature, the algae control activity can be greatly reduced at low illumination and low temperature, and the water-soluble copper and the complex thereof are basically ineffective even when the water temperature is lower than 15 ℃. The two are mixed to exert respective advantages, and simultaneously, the use amount of the two is reduced, thereby achieving the purpose of reducing the weight and improving the efficiency.

According to the invention, the water-soluble copper and the complex thereof are matched with the herbicide for use, compared with the dosage of a single agent, after the dosage of a single component 1/3 in the mixed agent is mixed, the generated synergistic effect is superior to the control effect of the single agent by 100%, the synergistic effect is realized, meanwhile, the quick action and the lasting period are well balanced, the control cost is greatly reduced, and the use cost is only 40-60% of the cost of the single agent.

Drawings

FIG. 1 shows the growth of moss after 7 days of treatment with different herbicides in test example 1;

fig. 2 is the moss microstructure of fig. 1, note: x10, observation under microscope for magnification of 10 times; x40, Observation under microscope for magnification of 40 times;

FIG. 3 is a graph comparing CK and E50 in test example 2 after administration for 5 days;

FIG. 4 is a graph comparing CK and E75 after 5 days of administration in test example 2;

FIG. 5 is a graph comparing T200 and T150 after 5 days of administration in test example 2;

FIG. 6 is a graph comparing T200 and E150 after 5 days of administration in test example 2;

FIGS. 7-9 are comparative images before and after application of the clear water control area of test example 3 (before application on the left side and after application on the right side);

FIGS. 10-12 are comparative plots before and after administration of the sweet and clear 0.8ppm treatment area of test example 3 (left plot before administration and right plot after administration);

FIGS. 13-15 are comparative plots of test example 3 before and after administration of a 0.3ppm sweet and clear + isoproturon 0.3ppm treatment area (left plot before administration and right plot after administration);

FIG. 16 is the appearance of the growth of Microcystis aeruginosa in the control treatment of experiment 4 for 0-168 hours;

FIG. 17 is the apparent growth of Microcystis aeruginosa treated with prometryn at 3ppm for 0-168h in test example 4;

FIG. 18 is the appearance of Microcystis aeruginosa growing in 0-168 hours after isoproturon treatment at 3ppm in test example 4;

FIG. 19 is the appearance of Microcystis aeruginosa growing in 0-168 hours after 3ppm treatment of chlortoluron in test example 4;

FIG. 20 is the appearance of Microcystis aeruginosa growing after 0-168 hours of 0.3ppm atrazine treatment in test example 4;

FIG. 21 is a graph showing the OD value changes of Microcystis aeruginosa in each treatment of 0 to 168 hours in test example 4.

Detailed Description

The present invention is described in further detail below with reference to specific examples in order to better understand the invention.

Formulation example 1: 400g/L of copper sulfate pentahydrate-isoproturon soluble liquid, wherein the content of copper sulfate pentahydrate is 300g/L, the content of isoproturon is 100g/L, the content of dimethylformamide cosolvent is 10 percent, the content of Tween 80 is 3 percent, the content of agricultural emulsion is 2 percent, and the balance of water is 100 percent.

Formulation example 2: 80% of anhydrous copper sulfate-chlortoluron water dispersible granule, wherein the anhydrous copper sulfate content is 60%, the chlortoluron content is 20%, the sodium lauryl sulfate is 1.5%, the Terwet 1004 content is 1.5%, the sodium carboxymethyl starch is 2%, the Morwet D425 content is 2%, and the kaolin is the balance of 100%.

Formulation example 3: 70 percent triethanolamine copper pendimethalin wettable powder, wherein the triethanolamine copper content is 55 percent, the pendimethalin content is 15 percent, the agricultural emulsion OX-656 content is 2 percent, the MF dispersant is 4 percent, and the talcum powder is added to 100 percent.

Formulation example 4: 40% of copper acetate-prometryn missible oil, wherein the copper acetate content is 30%, the prometryn content is 10%, the alkylphenol formaldehyde resin polyoxyethylene ether is 3%, the sucrose glyceride is 2%, and the naphtha is added to 100%.

Formulation example 5: 80% of copper gluconate-diuron water dispersible granules, wherein the copper gluconate content is 70%, the dioctyl sodium sulfosuccinate is 2%, the sodium glycocholate is 1%, the methylene dinaphthalene sulfonate is 1%, and the anhydrous sodium sulfate is added to make up 100%.

Formulation example 6: 40 percent of complex ammonia copper and atrazine suspending agent, wherein the complex ammonia copper is 30 percent, the atrazine is 10 percent, the styryl phenol polyoxyethylene ether is 2 percent, the content of agricultural emulsion 601# is 1 percent, the epichlorohydrin is 3 percent, the sodium carboxymethylcellulose is 1 percent, the propylene glycol is 4 percent, the tributyl phosphate is 0.1 percent, and the water is added to 100 percent.

Formulation example 7: 1.5 percent of copper chloride-simetryn granules, wherein 1 percent of copper chloride, 0.5 percent of simetryn, 20 percent of adsorbent diatomite, 1 percent of dispersant fatty alcohol-polyoxyethylene ether, 2 percent of binder bentonite, 0.1 percent of warning color acid scarlet, and attapulgite for making up 100 percent of the carrier.

Formulation example 8: 25% of copper citrate-metribuzin microcapsule suspending agent, wherein the content of copper citrate is 22%, the content of metribuzin is 3%, the content of polyurea is 3%, the content of a capsule core solvent is 5% of solvent oil 150#, the emulsifier agricultural emulsion OX-6562%, the stabilizer epichlorohydrin is 0.5%, the dispersant methylene dinaphthalene sulfonate (NPF) is 2%, the xanthan gum XG is 0.5%, the antifreeze propylene glycol is 3%, the defoamer organic silicon emulsion is 0.1%, the water is 100%, and an interfacial polymerization method is adopted.

Test example 1: herbicide indoor drug effect screening for moss

On the basis of earlier researches, the algae killing effect of the pendimethalin and copper sulfate in a mixed mode is researched, and the prevention effect of the oxadiazon, oxyfluorfen, diuron, bromoxynil octanoate, clomazone, oxaziclomefone and the combination of a plurality of medicaments on moss is explored.

Test method

Putting 0.25g of washed spirulina into a plastic cup, adding 150mL of tap water, and adaptively culturing for 3 d; preparing 1000ppm stock solution of each herbicide, and adding oxadiazon, oxyfluorfen, diuron, bromoxynil octanoate, clomazone, oxaziclomefone, pendimethalin, 0.50ppm of sweet and clear, 1.00ppm of sweet and clear: pendimethalin in a ratio of 0.5:0.25ppm, 0.5:0.5ppm, 0.5:1.0ppm, 1.0:10ppm, bromoxynil octanoate: the ratio of pendimethalin is 0.5:0.5ppm, 1.0:1.0ppm, 2 replicates per treatment, and an equivalent amount of clear water was added as a control. Culturing at 25-30 deg.C under the condition of light-dark time ratio of 12h to 12 h. The prevention and treatment effect of the agent on lichen is evaluated by visual inspection, as shown in fig. 1.

The copper sulfate sample used in the test is 410g/L of blue vitriol aqueous solution, the copper content is 10g/L, and the trademark is

The manufacturer is Sanjiang Probiotics, Inc.

The test results are shown in table 1, and the structure shows that:

1) this test showed that copper ions were still the fastest, in terms of rapid onset of yellowing of lichen;

2) the effect of the low-dose pendimethalin on the aged algae is not obvious, but the algae is in a shrinkage state after the application of the pendimethalin, and the pendimethalin can inhibit the newly grown algae;

3) after the pendimethalin and the copper sulfate are mixed, the integral etiolation speed is the same as that of the single use of the copper sulfate, but the control effect on the algae is better than that of the single use of the copper sulfate and the pendimethalin, and the synergistic effect is realized. Particularly, the effect of the two low-dose combined treatments is superior to that of the high-dose single-dose treatment;

4) the diuron has obvious inhibition on the photosynthesis of the Zhangban algae (no air bubbles are generated after the algae is used), and the moss can be settled within one day, but the yellowing is not as sweet and clear; oxyfluorfen has effect in inhibiting mature algae, and can also inhibit moss growth and reduce bubbles;

5) the other medicines have no obvious prevention and treatment effect on the moss under the test concentration.

TABLE 1 Change of Spirulina 1-7 days after treatment with each agent

Test example 2: field efficacy test of spirogyra control agent for crab rice field

In order to understand the action mechanism of the copper preparation and the isoproturon single preparation on the moss, the effect of the copper preparation and the isoproturon single preparation on the moss is observed, and the influence on the crab growth is also observed. A water environment protection research project group of Ningbo Sanjiang agricultural chemistry Limited company in 6 months in 2020 performs a field efficacy verification test on eight farms in Tangshan city Cao Fei Dian in Hebei province.

Test crops and varieties: rice- -variety jin source 89 (5-month 25-day transplanting, mechanical transplanting, rice row spacing 16 cm); river crabs, namely Chinese mitten crabs (the stocking density is 700/mu, and the specification of the stocking of the river crabs is 140/kg).

Test time: 4 days in 6 months of 2020 to 9 days in 6 months of 2020

The control object is: spirogyra sponge

Reagent to be tested: 410 g/L aqueous solution of cupric sulfate pentahydrate (Ningbo Sanjiang beneficial agricultural chemical Co., Ltd., trademark)

) (ii) a A 50% isoproturon wettable powder (manufactured by Jiangsu Kuaida Co.); 60% copper sulfate pentahydrate-20% isoproturon wettable powder (Cu: isoproturon 15:20, manufactured by Ningbo Sanjiangyi agricultural chemical Co., Ltd.).

Soil type: and (4) black loam.

And (3) experimental design: the test adopts the plot test, the test fields are separated by building ridges, 11 treatments are arranged, the treatment is repeated for 3 times, the total number of 33 plots is calculated, the area of each plot is 25 square meters, and the plots are arranged randomly.

Treatment 1:

50 ml/mu (T50-1, T50-2, T50-3);

and (3) treatment 2:

75 ml/mu (T75-1, T75-2, T75-3);

and (3) treatment:

100 ml/mu (T100-1, T100-2, T100-3);

and (4) treatment:

150 ml/mu (T150-1, T150-2, T150-3);

and (4) treatment 5:

200 ml/mu (T200-1, T200-2, T200-3);

and (6) treatment: isoproturon 50 g/mu (E50-1, E50-2, E50-3);

and (7) treatment: isoproturon 75 g/mu (E75-1, E75-2, E75-3);

and (4) treatment 8: isoproturon 100 g/mu (E100-1, E100-2, E100-3);

and (4) treatment: isoproturon 150 g/mu (E150-1, E150-2, E150-3);

and (4) treatment 10: 45 g/mu (Z-1, Z-2 and Z-3) of 60% copper sulfate pentahydrate and 20% isoproturon wettable powder;

and (3) treatment 11: blank (no agent administered).

The application method comprises the following steps: applying the medicine 1 time in 6 months and 4 days in 2020, spraying with 5L manual sprayer with spraying amount of 1L/25m²。

The investigation method comprises the following steps: investigating 3 times 1, 3 and 5 days after the application, visually observing the control effect according to the coverage of the spirogyra in the cell, fishing out the survival spirogyra by a five-point sampling method (20 cm per point by 30cm) 3 days after the application, and weighing to calculate the fresh weight control effect. The phytotoxicity of each treatment on rice was observed 1, 3, and 5 days after application.

Fresh weight control (%) - (blank zone biomass-treatment zone biomass)/blank zone biomass × 100%.

And (3) test results:

the control effect of the three agents on the paddy field spirogyra of the crabs is shown in a table 2, and the individual comparison graphs are shown in figures 3 to 6.

TABLE 2 preventing and killing effect of three kinds of chemical on the spirogyra in crab rice field

The test results in table 1 show that 80% copper sulfate pentahydrate-isoproturon wettable powder has the best prevention and removal effect on the spirogyra in the crab rice field, the quick action is better than that of 50% isoproturon wettable powder single agent, the treatment is consistent with that of 410 g/L copper sulfate pentahydrate aqueous solution, but the final duration is consistent with that of 50% isoproturon wettable powder high dose. As can be seen from the control effect of the coverage degree of 3 days after the pesticide is applied, the control effect of the 50 percent isoproturon wettable powder to the spirogyra is over 90 percent, and the difference among the concentrations is not obvious; the prevention effect of 410 g/L copper sulfate pentahydrate aqueous solution on spirogyra increases with the increase of the concentration. The fresh weight control effect of 3 days after the pesticide is more than 60 percent, comprising all treatments of 410 g/L of blue vitriol aqueous solution 150 ml/mu and 200 ml/mu and 50 percent isoproturon wettable powder, wherein the control effect of 410 g/L of blue vitriol aqueous solution 150 ml/mu is 70.48 percent best, and the control effect of 50 percent isoproturon wettable powder 150 ml/mu is 91.12 percent best.

The experimental result shows that the combination of the two has obvious synergistic effect, and the prevention effect can be achieved only by high-dose treatment of single copper sulfate or single isoproturon with small dosage of the mixed agent.

The investigation result of the rice phytotoxicity and the crab safety is as follows: no adverse effect of the test agent on the growth and development of the rice is observed in the test period, and the test agent is safe and harmless to the rice. No effect on crabs was observed.

The test results of the test example show that the three agents have certain control effects on spirogyra, wherein the 80% copper sulfate pentahydrate-isoproturon wettable powder has the best overall control effect, and a better synergistic effect can be shown by using a lower dosage. In the process of investigation and test results, isoproturon has good control effect on the bottom layer newly-grown spirogyra, so that the fresh weight control effect is better than that of 410 g/L blue copperas aqueous solution. The control effect of 410 g/L copper sulfate pentahydrate aqueous solution 150 ml/mu is inferior to that of the former two, and in the process of investigation test results, the control effect of 410 g/L copper sulfate pentahydrate aqueous solution on surface spirogyra is found to be superior to that of bottom layer new growth spirogyra, so the effect can be seen after 1 day of pesticide application, the coverage control effect is superior to that of 50% isoproturon wettable powder after 1 day of pesticide application, but the whole control effect is inferior. In the control period, it is recommended to apply the pesticide at the initial stage of spirogyra generation, so that the control effect is better.

Test example 3: autumn and winter formula algaecide field test

The purpose is as follows: the field efficacy of the formula medicament is verified through a field efficacy test.

Test time, place, conditions: in 2020, the temperature is 20 ℃ and the average width of the circular trench is 4m and the depth is 0.5m from 11 months 15 to 11 months 18 days.

Test materials

Test targets: algal bloom of moss in a culture water area caused by spirogyra, Zhangban algae, Nemacystus aquaticus and the like;

test agents: sweet clear (410g/L aqueous copper sulfate pentahydrate), sweet clear + isoproturon;

an experimental instrument: a 100 ml measuring cylinder, a small electronic balance, a measuring tape (50 m), a meter ruler (5 m) and an electric sprayer.

The test method comprises the following steps: the test point shows that the moss coverage is over 50 percent, the medicament is prepared after the water amount of the water body of the circular groove is calculated, and sweet and clear water is respectively applied to two opposite circular groove water bodies by adopting a spraying and applying mode, wherein the sweet and clear water is 0.8ppm, and the sweet and clear water is 0.3ppm plus isoproturon is 0.3 ppm; equal amounts of clear water were applied to both lateral sulci as a control treatment. The test record is shown in Table 3.

And (4) investigating a result: and 3 observation points are selected from each processed ring ditch, and the algae control effect of the agent is evaluated by observing the moss coverage (including underwater and water surfaces).

The coverage control effect formula is as follows:

in the formula:

e-inhibition in units;

d0-mean coverage of moss before treatment;

dt-mean moss coverage after treatment.

And (3) test results: compared with a clear water control area, the control effect of 0.8ppm sweet and clear is very obvious, and the control effect reaches 92.1%; the control effect of 0.3ppm of sweet and clear and 0.3ppm of isoproturon is obviously superior to that of the treatment of the sweet list agent, and the control effect reaches 99.2 percent; the control effect on the floating moss and the underwater moss is very thorough.

TABLE 3 record of the tests

Test example 4: control effect and duration comparison test of isoproturon, chlortoluron, prometryn and atrazine on microcystis aeruginosa

The purpose is as follows: the control effect and the control duration difference of the isoproturon, the chlortoluron, the prometryn and the atrazine on the microcystis aeruginosa are determined through tests.

Time: 22/10/2019-22/2020/10/2020

Test materials: test targets: microcystis aeruginosa (FACHB-911) is purchased from the algal seed bank of Chinese academy of sciences, and is cultured in BG-11 culture medium in a room at the temperature of 25 +/-1 ℃ and the photoperiod of 12h:12h, shake flask culture, shake 2 times daily.

Test agents: 50% isoproturon wettable powder, 25% chlorotoluron wettable powder, 40% prometryn wettable powder and 38% atrazine suspending agent.

The test method comprises the following steps: transferring 300mL of well-grown algae solution into 500mL of sterilized BG-11 culture medium under aseptic conditions, performing shake culture at 25 +/-1 ℃ for 12h indoors under 12h illumination for 1 day, and subpackaging into 100mL triangular flasks with 50mL of algae solution per flask. The agents were added under sterile conditions: isoproturon, chlortoluron, prometryn, atrazine 0.3 ppm; each treatment was repeated 3 times, and shaking culture was continued (a small amount of culture solution was periodically supplemented to the system). The OD value of each treatment (OD value corresponds to the number density of algae cells, the larger the OD value is, the larger the number of algae cells is) is measured under the aseptic condition at No. 1, 15, 30, 60, 120, 240 and 360d respectively, and the relative inhibition rate of the treatment on the microcystis aeruginosa is calculated.

IR ═ control OD value-treatment OD value)/control OD value

And (3) test results: the OD value of the microcystis aeruginosa for 0-360 days of each treatment is shown in the table 4; the inhibition rate of each treatment for 0-168h on the growth of the microcystis aeruginosa is shown in table 5; the OD value of Microcystis aeruginosa in each treatment was varied from 0 to 168 hours, as shown in FIG. 21.

The four herbicides show better inhibition effect on microcystis aeruginosa in the early stage (within 15 d), and the cell number of algae can be controlled at a lower level; controlling isoproturon for 15-30 days, and recovering the cell number of algae to the control level after 30 days; the control time of chlortoluron is about 60-120d, and the number of algae cells is recovered to the control level after 120 d; the control time of prometryn and atrazine exceeds 240 d; the appearance of growth of Microcystis aeruginosa is shown in FIG. 16-FIG. 20.

TABLE 4 treatment of 0-360 days on growth OD of Microcystis aeruginosa (the larger the OD, the more the number of algal cells)

TABLE 5 inhibition rate of each treatment for 0-168h on growth of Microcystis aeruginosa

Claims

1. The algae-controlling composition is characterized by comprising two active components, namely a component A and a component B, wherein the component A is water-soluble copper and a complex thereof, the component B is a herbicide, and the weight part ratio of the component A to the component B is 0-99: 99-0.

2. The algae control composition of claim 1, wherein the weight ratio of copper in component a to herbicide is 0-50: 60-0.

3. The algae control composition of claim 1, wherein the weight ratio of copper in component a to herbicide is from 0.5-20: 50-1.

4. The composition according to claim 1, 2 or 3, wherein the water soluble copper and its complex comprises any one or more of copper chloride, copper sulfate, copper nitrate, copper citrate, copper gluconate, copper ammine, copper acetate, copper ethanolamine, copper triethanolamine, copper ethylenediamine double hydroxide, copper ethylenediamine, copper rosinate, and copper amino acid.

5. An algicidal composition according to claim 1, 2 or 3 wherein the herbicide is a mixture of one or more of substituted urea, dinitroaniline and triazine herbicides, the herbicides being a mixture of the same type of herbicide or a mixture of different types of herbicides.

6. The composition of claim 5, wherein the substituted urea herbicide is any one of isoproturon, chlorotoluron, diuron, fluometuron, glusulfuron, and triflusuron.

7. The algae control composition of claim 5, wherein the dinitroaniline herbicide is any one of pendimethalin, butralin, and trifluralin.

8. The composition according to claim 5, wherein the triazine herbicide is any one of prometryn, simetryn, atrazine and metribuzin.

9. The composition according to any one of claims 1 to 8, wherein the composition can be made into any one of suspension, water dispersible granule, wettable powder, granule, soluble liquid, emulsifiable concentrate, microemulsion, oil suspension, suspoemulsion, microcapsule suspension.

10. An algae control composition for use in controlling excessive algae proliferation in rivers, lakes, rice fields, ponds, reservoirs, rivers, landscape architecture, fish tanks, fish ponds, latex and paint sites and materials.