CN102577891B - Application of Amblyseius orientalis Ehara in preventing and controlling Bemisia tabaci and Thrips - Google Patents

Application of Amblyseius orientalis Ehara in preventing and controlling Bemisia tabaci and Thrips Download PDF

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CN102577891B
CN102577891B CN 201110456704 CN201110456704A CN102577891B CN 102577891 B CN102577891 B CN 102577891B CN 201110456704 CN201110456704 CN 201110456704 CN 201110456704 A CN201110456704 A CN 201110456704A CN 102577891 B CN102577891 B CN 102577891B
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bemisia tabaci
thrips
east
ehara
genus amblyseius
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CN102577891A (en
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王恩东
王伯明
徐学农
姜晓环
胡毅
盛福敬
马鹤娟
王培�
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SHOUBONONG (BEIJING) BIOTECHNOLOGY CO., LTD.
Institute of Plant Protection of Chinese Academy of Agricultural Sciences
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Abstract

The invention relates to new application of Amblyseius orientalis Ehara in preventing and controlling Bemisia tabaci and Thrips, applicable to greenhouse, tea garden, cotton field, orchard and farmland, and realized through broadcasting. The method includes releasing Amblyseius orientalis Ehara with an amount of more than or equal to one Amblyseius orientalis Ehara per square meter each time every 7-30 days during early breakout of Bemisia tabaci and Thrips. The invention breaks through the misunderstanding that Amblyseius orientalis Ehara is predator specific for spider mite, achieves unexpected effects, and solves difficult treatment/control of Bemisia tabaci by predatory mites. It is demonstrated that Amblyseius orientalis Ehara is applicable to treatment and control of spider mite, Bemisia tabaci and Thrips. The application of Amblyseius orientalis Ehara can effectively reduce harm of Bemisia tabaci and Thrips, lower application of pesticides, and improve product quality.

Description

Use the purposes that the east genus amblyseius berlese is prevented and treated Bemisia tabaci, thrips
Technical field
The present invention relates to use the new purposes of Bemisia tabaci, thrips in the farm-forestry crops such as genus amblyseius berlese control greenhouse, east, tea place, cotton, farmland, belong to pest biological control field.
Technical background
The east genus amblyseius berlese belongs to Phytoseiidae (Phytoseiidae), genus amblyseius berlese subfamily (Amblyseiinae), genus amblyseius berlese genus (Amblyseius), have a very wide distribution in China, in Hebei, all there is distribution in Tianjin, Liaoning, Jiangsu, Anhui, Fujian, Jiangxi, Shandong, Hubei, Hunan, Guangdong and Guizhou, perching crop has apple tree, orange tree, peach, pear tree, jujube tree, soybean etc.It can effectively prevent and treat citrus red mite (crm) (Panonychus citri), panonychus ulmi (Panonychus ulmi), tetranychus viennensis (Tetranychus viennensis), cypress unguiculus mite (Oligonychus perditus) etc., being the considerable advantage kind predation mite in the tetranychid control, also is a kind of effective sociales natural enemy in the citrus garden [1]Studies show that the east genus amblyseius berlese is fine to the control effect of citrus red mite, can control panonychus ulmi and tetranychus viennensis fully.Yang Ziqi etc. [2]Discharge the test of east genus amblyseius berlese control citrus red mite in the tangerine tree and show that discharge inspection after 5 days, the citrus red mite (crm) insect population descends and reaches 68.86%; Zhang Shouyou etc. [3]Discharge east genus amblyseius berlese control panonychus ulmi and tetranychus viennensis test in the apple orchard and show that the east genus amblyseius berlese can be controlled these two kinds harmful mites fully under natural situation, control efficiency reaches 93.4%.
Yet it is contained according to the existing literature data, the east genus amblyseius berlese is only preyed on tetranychid and as the biological control thing of tetranychid, therefore, it is habitually thought the special feeding habits natural enemy of tetranychid always, this viewpoint one piece of paper " quantitative analysis of red fuji apple garden insect and natural enemy group " that domestic one-level academic journal " Chinese Journal of Applied Ecology " was delivered in 2009 [4]In also obtain approval, support.
Bemisia tabaci (Bemisia tabaci), thrips are important in the world dangerous insects, not only by direct thorn suction water, cause the dehydration of host plant chlorosis, influence the accumulation of photosynthesis and crop nutrition content; And, also can propagate the viroses of plant, various crop such as vegetables, flowers etc. are caused crushing harm.Wherein China's Bemisia tabaci is based on B-type Bemisia tabaci, and the type Bemisia tabaci is the pest of unique being called as " super insect " at present in the world, also is one of insect of the refractory reason of China; Frankliniella occidentalis in the thrips (Frankliniella occidentalis) is the external harmful biological invasion of China still.Mostly the method for now preventing and treating Bemisia tabaci, thrips is chemical control, a large amount of uses of chemical pesticide cause Bemisia tabaci, thrips that chemical pesticide is produced resistance, namely occur: the usage amount of chemical pesticide and working concentration all constantly increase, and make chemical control enter vicious circle.A series of crises such as that a large amount of uses of agricultural chemicals also cause is residual, food security and ecological safety.Therefore, biological control becomes the important measures of the improvement pest that national governments and scientist actively seek.
The predation mite is the important natural enemy of preventing and treating Bemisia tabaci, thrips, abroad the little blind peaceful mite of commercial Si Shi (Typhlodromips swirskii) is present unique predation mite kind that can be used for preventing and treating aleyrodid in the world, it also can be used for the control of thrips simultaneously, but the little blind peaceful mite of Si Shi is in China's distribution-free, and China is this mite of formal introduction as yet, discharges to use and all can not carry out.The predation mite of the prevented and treated Bemisia tabaci that China now reports, thrips has amblyseius cucumeris oudeman (Amblyseius cucumeris), and (number of patent application 200910112506.0, Zhang Yanxuan 2011 [5]).Yet amblyseius cucumeris oudeman is from external introduction, and a little less than poor, the competitive ability of adaptive capacity, field investigation does not find that as yet this mite can grow surely in China in China, and the amblyseius cucumeris oudeman of release can't find in the field after producing season at one; Proposing amblyseius cucumeris oudeman in patent application, the document and can prey on Bemisia tabaci, but do not see the data history of life whether this mite can finish amblyseius cucumeris oudeman to predation amount and the Bemisia tabaci of each worm attitude of Bemisia tabaci, only is to observe just the sprouted wings situation of adult of predation Bemisia tabaci.
Vast territory and abundant resources in China, and predation mite kind is huge many, and the predation mite kind that can be used for the tetranychid control of wherein having excavated out is many, yet, the endemic species predation mite that China can be used for preventing and treating Bemisia tabaci and thrips simultaneously so far but the neither one kind discovered and used.Bemisia tabaci, thrips to the harm of farm-forestry crop and difficulty of prevention and cure not second to tetranychid, therefore do not have to be applied to simultaneously the endemic species predation mite of high-efficiency prevention and control Bemisia tabaci, thrips based on China now, need further to excavate simultaneously Bemisia tabaci, the high preventive effect of thrips tool, have more practicality and can be converted into the endemic species natural enemy predation mite of production application.
Yet, biological habit not of the same race varies in the predation mite colony, wanting to excavate out a kind of good predation mite in addition need also need to solve from filtering out to and raise each link of using through multiplex screening even thousands of tests, is quite very difficult.
In order to excavate out the how more effective predation mite that can be used for preventing and treating Bemisia tabaci, thrips simultaneously, we at home many provinces and cities constantly gather, with the aleyrodid screening of feeding, go through the several years kind predation mite surplus 110 is attempted having no resolution, find unexpectedly that but the east genus amblyseius berlese that always is considered to " the special feeding habits predation of tetranychid mite " has very strong prey ability to Bemisia tabaci and thrips.We are applied in greenhouse, tea place, cotton field, orchard, the farmland to be used for the control of Bemisia tabaci, thrips with the east genus amblyseius berlese, and result of the test shows that the east genus amblyseius berlese has good control efficiency to Bemisia tabaci, thrips.
The invention solves China's Bemisia tabaci and be difficult to a difficult problem with the predation acarid control, for the biological control of China's Bemisia tabaci provides a kind of native country at present unique natural enemy predation mite kind, and it also can be applicable to the control of insects such as thrips, tetranychid.
The present invention has obtained unexpected technique effect, because forefathers think that always the east genus amblyseius berlese is the predation mite that specially eats tetranychid!
It is the understanding mistake of a kind of special feeding habits predation mite to the east genus amblyseius berlese that the present invention has broken through forefathers, finds first, confirms that it except preventing and treating the tetranychid, also can be used for the control of Bemisia tabaci, thrips!
Summary of the invention
The objective of the invention is to provide for the agriculture and forestry injurious insect biological control the novel predation of endemic species natural enemy mite, i.e. the east genus amblyseius berlese of a kind of high-efficiency prevention and control Bemisia tabaci, thrips simultaneously.The present invention has overcome China and has had the predation mite kind poorness of preventing and treating Bemisia tabaci, thrips, the defective that prey ability is weak, colonazition is poor, practicality is not strong, has solved to lack the endemic species predation mite difficult problem of high-efficiency prevention and control Bemisia tabaci, thrips simultaneously; The present invention has also overcome technology prejudice, forefathers think that always the east genus amblyseius berlese only can prevent and treat tetranychid, be the special feeding habits natural enemy of tetranychid, the present invention has broken the understanding deficiency of forefathers to east genus amblyseius berlese predation feeding habits, and proposing the east genus amblyseius berlese first is the important natural enemy of Bemisia tabaci, thrips.The present invention can reduce the use of chemical pesticide, helps lend some impetus to the high-quality and safety production of agriculture and forestry product, belongs to a kind of method of completely newly preventing and treating of Bemisia tabaci, thrips biological control.
The present invention relates to the east genus amblyseius berlese and prevent and treat the new purposes of Bemisia tabaci, thrips, it is characterized in that the east genus amblyseius berlese is applied in the new purposes that greenhouse, tea place, cotton field, orchard, farmland prevent and treat Bemisia tabaci, thrips, using method is mainly to realize the release of east genus amblyseius berlese by the mode of broadcasting sowing, also can use together with the mode of hanging bag, be at Bemisia tabaci, thrips initial phase release time, discharge 1 east genus amblyseius berlese in every 7-30 days, discharge east genus amblyseius berlese 〉=1/square metre at every turn.
When described Bemisia tabaci, thrips are about to take place to refer in early days that foundation has just found to have Bemisia tabaci and thrips generation by inspection blade, observation insect-sticking plate.
50-2000 east genus amblyseius berlese of described every square metre of release refers to formulate burst size according to the extent of injury of Bemisia tabaci, thrips on the plant and the size of plant, Bemisia tabaci, thrips endanger serious 〉=2/Ye Shike forces down Bemisia tabaci, thrips earlier by dispenser quantity, discharge the east genus amblyseius berlese again.
The present invention namely uses the method that the east genus amblyseius berlese is prevented and treated Bemisia tabaci, thrips for the biological control of Bemisia tabaci, thrips provides a kind of brand-new method of preventing and treating.The east genus amblyseius berlese can not only be preyed on Bemisia tabaci, thrips ovum, also can prey on Bemisia tabaci, thrips larva, and the east genus amblyseius berlese day egg laying amount after the predation is the 1-2 grain, is at present domestic unique native country predation mite kind that can be used for effective prevention and control aleyrodid.The present invention proposes to discharge at Bemisia tabaci, thrips initial phase the scheme of predation mite, can earlier set up east genus amblyseius berlese population, reduces the chemical pesticide use, increase natural enemy kind quantity; The present invention is with the major measure of the mode of broadcasting sowing as pest control, thereby the submerged that can more substantially carry out the east genus amblyseius berlese discharges, and drops on the face of land although have part predation mite, and the east genus amblyseius berlese that drops on the face of land also can be diffused on the plant very soon.
Embodiment
Embodiment 1 uses the using method that the east genus amblyseius berlese is prevented and treated Bemisia tabaci
This method is mainly to realize the release of east genus amblyseius berlese by the mode of broadcasting sowing, also can use together with the mode of hanging bag, be at the Bemisia tabaci initial phase release time, discharged 1 east genus amblyseius berlese in every 7-30 days, can discharge together in conjunction with hanging bag, Bemisia tabaci amount 〉=2/Ye Shike discharges the east genus amblyseius berlese weekly 1 time, discharges east genus amblyseius berlese 50-2000 head/m at every turn 2
Embodiment 2 east genus amblyseius berlese are prevented and treated thrips in kind has the greenhouse of eggplant application and method
At the thrips initial phase, east genus amblyseius berlese and raising medium thereof are broadcasted sowing on the greenhouse eggplant plant together, the amount of broadcasting sowing of east genus amblyseius berlese is 〉=1: 1 with thrips amount ratio, the release bag that will contain 2000 east genus amblyseius berlese again is suspended on every eggplant plant, release bag contains the food of east genus amblyseius berlese, repeated said method in every month and discharge 1 time, can realize the application of east genus amblyseius berlese in greenhouse eggplant insect thrips biological control, control efficiency reaches more than 30%.
Application and the method for embodiment 3 east genus amblyseius berlese in the Bemisia tabaci biological control of cotton field
When estimating that the cotton field Bemisia tabaci is about to take place, broadcast sowing east genus amblyseius berlese and raising medium thereof on the cotton together, the amount of broadcasting sowing of east genus amblyseius berlese is 〉=1: 1 with insect amount ratio, the release bag that again 1-5 is included 2000 east genus amblyseius berlese is suspended on the cotton plants, release bag contains the food of east genus amblyseius berlese, repeat said method in every month and discharge 1 time, can realize the application of east genus amblyseius berlese in the Bemisia tabaci biological control of cotton field.
Should choose release time during release, note wind direction, before raining or raining, during high temperature, all be unsuitable for discharging the east genus amblyseius berlese when wind is big.
Because the extent of injury, geographical position, climatic environment difference, the release of predation mite is preferably carried out under professional and technical personnel's guidance.
Test is implemented 1 east genus amblyseius berlese to predation amount and the egg laying amount thereof of tobacco powder nit
Test method: under 25 ℃, 75% relative moisture condition, will directly receive on the kidney bean blade that contains the tobacco powder nit with the female one-tenth mite of east genus amblyseius berlese that Bemisia tabaci was raised two days, record predation amount, egg laying amount repeat 6 times behind the 24h.
The results are shown in Table 1, the east genus amblyseius berlese can be preyed on Bemisia tabaci and just lay eggs more than 4, and 1.3 of per day egg laying amounts illustrate that the east genus amblyseius berlese can prey on the tobacco powder nit and lay eggs.
Table 1 east genus amblyseius berlese is to day predation amount and the egg laying amount thereof of tobacco powder nit
Figure BSA00000648191800051
The research of 2 east genus amblyseius berlese predation Bemisia tabaci larvas and egg laying amount thereof is implemented in test
Whether test method: under 25 ℃, 75% relative moisture condition, genus amblyseius berlese female one-tenth mite in east is directly received on the kidney bean blade that contains the Bemisia tabaci low instar larvae, observed the Bemisia tabaci larva behind the 24h and reduce, the egg laying amount of record east genus amblyseius berlese repeats 9 times.
The result shows that the east genus amblyseius berlese can be preyed on the Bemisia tabaci larva, and it on average also is 1.33 (table 2) that day lays eggs.
The day egg laying amount of east genus amblyseius berlese when table 2 is raised with the Bemisia tabaci larva
List of references:
[1] Zhu Zhimin, Lai Yongfang. Chinese research and utilization predation mite overview, spider shape journal, 1992.
[2] Yang Ziqi, Cao Kejia, Li Weiping. east genus amblyseius berlese research preliminary study, insect natural enemy, 1987.
[3] Zhang Shouyou, Cao Xinwen, Han Zhiqiang, Wu Weinan. the east genus amblyseius berlese is to apple orchard two kinds of tetranychid natures control action research, insect natural enemy, 1992.
[4] Zheng Fangqiang, Zhang Xiaohua, Qu Chenghuai, Liu Xueqian, Qu Shujuan. the quantitative analysis of red fuji apple garden insect and natural enemy group, Chinese Journal of Applied Ecology, 2009.
[5] Zhang Yanxuan, woods faithfulness, Zhang Gongqian, Chen Xia, Ji Jie, Tang Qing. the research of amblyseius cucumeris oudeman control booth pimento Bemisia tabaci, Fujian agriculture journal, 2011.

Claims (1)

1. use the purposes that the east genus amblyseius berlese is prevented and treated Bemisia tabaci, thrips, it is characterized in that the east genus amblyseius berlese is applied in the new purposes that greenhouse, tea place, cotton field, orchard, farmland prevent and treat Bemisia tabaci, thrips, application method is at Bemisia tabaci, thrips initial phase, discharged 1 east genus amblyseius berlese in every 7-30 days, each burst size 〉=1/square metre.
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CN103299959A (en) * 2013-05-20 2013-09-18 中国农业科学院植物保护研究所 Novel producing method of predatory mites
CN104521471A (en) * 2014-12-04 2015-04-22 中国农业科学院棉花研究所 Identification method of high-resistance and high-sensitivity bemisia tabaci standard material of semi-wild cotton
CN105075745A (en) * 2015-08-18 2015-11-25 中国农业科学院植物保护研究所 Method for preventing and controlling spider mites of horticultural plants through predatory mites
CN116035036B (en) * 2023-03-27 2023-05-30 中国农业大学 Application of neoseiulus neoseius in prevention and treatment of leguminous plants, namely, bean thistle and horse
CN116326400A (en) * 2023-04-13 2023-06-27 福建省农业科学院植物保护研究所 Application of amblyseius pseudopterogorus in preventing and controlling tea orange goiter and method thereof

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