CN116326400A - Application of amblyseius pseudopterogorus in preventing and controlling tea orange goiter and method thereof - Google Patents

Application of amblyseius pseudopterogorus in preventing and controlling tea orange goiter and method thereof Download PDF

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CN116326400A
CN116326400A CN202310391718.7A CN202310391718A CN116326400A CN 116326400 A CN116326400 A CN 116326400A CN 202310391718 A CN202310391718 A CN 202310391718A CN 116326400 A CN116326400 A CN 116326400A
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amblyseius
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陈霞
孙莉
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Institute of Plant Protection of FAAS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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Abstract

The invention provides application of amblyseius pseudopteropi in preventing and controlling tea orange gall mites and a method thereof, and belongs to the technical field of biological prevention and control of agricultural pests. The method of the invention mainly controls the harm of tea orange gall mites by releasing amblyseius pseudoptera in tea gardens in 4 to 9 months each year. The method of the invention not only can effectively prevent and control the damage of the tea orange goiter, but also has the advantages of simplicity, high efficiency, long lasting time, reduced pesticide residues, improved tea yield and quality and ecological environment protection.

Description

Application of amblyseius pseudopterogorus in preventing and controlling tea orange goiter and method thereof
Technical Field
The invention belongs to the technical field of biological control of agricultural pests, and particularly relates to application of amblyseius pseudopteropi in controlling tea orange gall mites and a method thereof.
Background
Tea is one of three major drinks in the world, and the number of tea-producing countries and regions worldwide reaches more than 60. Tea originates in China and has been deeply integrated into the life of Chinese people, and becomes an important carrier for inheriting Chinese culture. Tea orange gall mites are one of the most important mites which harm tea trees, and are harmful to adult moths and young mites by sucking tea tree juice through needle mouthparts. The damaged leaves are yellow and green, the main veins of the leaves are red, the leaves lose luster, brown rust spots appear on the backs of the leaves when the leaves are seriously damaged, buds She Weisu are dry and fire-like, a large number of fallen leaves are caused, the yield, the quality and the tree vigor of the tea leaves are seriously influenced, and even no tea can be picked. For a long time, tea farmers use chemical pesticides for controlling, which results in increasing drug resistance and dosage of pests year by year. The use of pesticides in large quantities not only pollutes the environment, but also causes the problem of pesticide residue of tea, influences the quality of tea, damages the ecological environment and causes environmental pollution, and the health of consumers is hidden for a long time.
The amblyseius pseudopterus belongs to the arachnidae, phylum consignus, phytoseiidae, amblyseius, chant was originally named Typhlodromus (Amblyseius) asetus in 1959 and Proprioseiopsis asetus (Chant) in 1986, and the academic name has been used until now. Is mainly distributed abroad in the united states (virginia, california), brazil, mexico, jamaica, etc.; it is reported in China that it is distributed in Jiangsu, guangdong, guangxi, taiwan and Jiangxi. The amblyseius pseudoplectania is a predatory mite with wider distribution, can predatory pests such as thrips tabaci, thrips palmi, thrips frankliniella, tebufenella radicata, tetranychus eastern, paederia triangularis, and the like, can ingest various plant pollens, and is an excellent local natural enemy predatory mite. Although there are many reports of controlling various pests and mites by using various predatory mites, there are no reports of application of the amblyseius pseudophakii in controlling tea orange gall mites at home and abroad.
Disclosure of Invention
Therefore, the invention aims to provide the application of the amblyseius pseudopteropimaricus in preventing and controlling the tea orange goiter mites and the method thereof, which can effectively prevent and control the damage of the tea orange goiter mites and improve the yield and quality of tea.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides application of amblyseius pseudopteropi as natural enemy in preventing and controlling tea orange gall mites.
The invention also provides a method for preventing and controlling the tea orange goiter, and the method releases the amblyseius pseudopterus in tea gardens 4-9 months each year.
Preferably, 15 to 20 ten thousand amblyseius pseudofront are released per mu.
Preferably, the release conditions are: the average pest and mite amount in each leaf of the tea garden is lower than 2.
Preferably, the release mode of the amblyseius pseudofront in the tea garden is submerged release.
Preferably, the submerged release method is as follows: the wheat bran or the chaff is used for uniformly mixing the amblyseius pseudopteropi, and then uniformly spreading the mixed amblyseius pseudopteropi on the leaf surfaces of the tea trees.
Preferably, the submerged release time is: release in the morning and evening on sunny days or release in the whole cloudy day.
Preferably, the amblyseius pseudopleomorphus is not mixed with pesticides and fertilizers.
Preferably, after releasing amblyseius pseudoplectania, the tea garden does not use herbicide or high-toxicity chemical pesticide.
The invention has the beneficial effects that:
the invention discovers that the amblyseius pseudopteropis can eat tea orange goiter mites for the first time, and uses the amblyseius pseudopteropis as natural enemy for preventing and controlling the tea orange goiter mites. The invention releases the amblyseius pseudolarieius for 1 time a year, has simple operation, and can control the yellow tea mites, the white coffee mites, the tea short-hair mites, the tea gall mites and other harmful mites in tea gardens besides preventing and controlling the tea orange gall mites by using the natural enemy amblyseius pseudolarius. Experiments show that the pesticide has good use effect, the control effect is more than 85%, and the use of chemical pesticides is reduced by 4-8 times in a year.
The amblyseius pseudopterus biocontrol garden releasing natural enemy has the advantages of reducing the use of chemical pesticides, protecting natural enemies, playing the role of the natural enemies, optimizing and enhancing the ecological system function of the biocontrol garden, protecting the ecological environment, achieving the lasting control effect and realizing the sustainable control of pests. And on the basis of effectively preventing and controlling the damage of the tea orange goiter, the method has the advantages of improving the yield and quality of tea and protecting the ecological environment.
Drawings
Fig. 1: releasing a amblyseius pseudofront amblyseius biocontrol garden and chemical prevention garden tea orange gall mite population growth curve;
fig. 2: releasing a amblyseius pseudofront amblyseius biocontrol garden and chemical prevention garden tea orange gall mite population growth curve;
fig. 3: the amblyseius pseudopterus preys tea orange goiter;
fig. 4: an enlarged view of amblyseius pseudopterus predatory tea orange gall mites;
fig. 5: tea garden in Chang county of Jian nan Ping City of Fujian province;
fig. 6: tea garden in Shunchang county of south Pingzhi of Fujian province;
fig. 7: the tea garden releases amblyseius pseudofront.
Detailed Description
The invention provides application of amblyseius pseudopteropi as natural enemy in preventing and controlling tea orange gall mites.
The invention discovers that the amblyseius pseudopteropi can eat tea orange gall mites (figure 3 and figure 4) for the first time, and can effectively prevent and treat the tea orange gall mites by releasing the amblyseius pseudopteropi as natural enemies to tea trees. The invention has no special limitation on the source of the amblyseius pseudopleomorphus, and can be obtained by artificial feeding or collection.
The invention also provides a method for preventing and controlling the tea orange goiter, which selects 4-9 months each year to release the amblyseius pseudopteropi in the initial stage of the development of the tea orange goiter. The amblyseius pseudopleomorphus needs to be released when the pest mite density is low, and the release conditions are as follows: the average pest and mite amount in each leaf of the tea garden is lower than 2. When the average pest amount of pest mites per leaf is found to be higher than 2, biological pesticides or low-toxicity pesticides are selected for controlling once, and after 5-7 days, the average pest amount of pest mites is lower than 2, and predatory mites are released.
The release amount of the amblyseius pseudopleomorphus is 15-20 ten thousand, preferably 17-19 ten thousand, more preferably 17 ten thousand per mu.
The release mode of the amblyseius pseudofront in the tea garden is submerged release. The submerged release method comprises the following steps: the wheat bran or the chaff is used for uniformly mixing the amblyseius pseudopteropi, and then uniformly spreading the mixed amblyseius pseudopteropi on the leaf surfaces of the tea trees. As an alternative implementation mode, the invention uniformly mixes the amblyseius pseudopterus with the consumption of 15-20 ten thousand per mu with 2-3 kg of wheat bran or chaff, and then uniformly spreads the mixed amblyseius pseudopterus on the leaf surfaces of tea trees in 1 mu of tea garden.
In the invention, the amblyseius pseudopterus is not mixed with pesticide and chemical fertilizer. After releasing the amblyseius pseudolaris, the tea garden does not use herbicide or high-toxicity chemical pesticide, and the shallow root system weeds are reserved or the grass growing cultivation is carried out, so that the amblyseius pseudolaris and other natural enemies are ensured to keep natural ecological environment.
The technical solutions of the present invention will be clearly and completely described in the following in connection with the embodiments of the present invention. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the following examples, conventional methods are used unless otherwise specified.
Materials, reagents and the like used in the examples described below are commercially available unless otherwise specified.
Example 1
Biocontrol region: in the tea garden of Shunchang county in southern Pingcity of Fujian province, a region which is about 50 mu of relatively independent and serious in-year tea orange goiter mite is selected as an experimental object, the tree age of the tea garden is 20 years, 5-point sampling methods are adopted during investigation, 5 new shoots are selected in the east, the west, the south, the north and the middle of the tea garden respectively, the tea garden is brought back to a laboratory for observation and inspection under a microscope, 3 leaves are investigated from top buds downwards every a little, 75 leaves are investigated in total, and the total quantity of all insect states of the tea orange goiter mites is counted.
15 ten thousand amblyseius pseudopleomorphus are released per mu in 4 months and 15 days, and after adding 15 ten thousand amblyseius pseudopleomorphus into 3 kg of dried wheat bran, uniformly mixing, uniformly spreading on the leaf surfaces of tea trees in a tea garden. The investigation was done every 10 days after release. The release mode is as follows: 15 ten thousand amblyseius pseudoplectania is added into 3 kg of dried wheat bran, uniformly mixed and uniformly spread on the leaf surfaces of tea trees in a tea garden with 1 mu (figure 7).
After release of amblyseius pseudopterogorus, no herbicide and chemical pesticide was used.
Chemical defense area: and a 10 mu conventional tea-proof garden is selected in the same place as a control, and the investigation method is the same as above. The chemical prevention garden is respectively used for preventing and controlling at 4 months 15 days by 15% pyridaben 1500 times liquid, 6 months 15 days by 73% propargite 1500 times liquid, 8 months 15 days by 0.6% clear source protection 800 times, 9 months 15 days by 99% green (spraying) 150 times liquid, 10 months 15 days by 15% fast mite emulsifiable concentrate 250 ml/mu, 11 months 15 days by 1% imazethapyr emulsifiable concentrate 20 ml/mu.
The control effect of the biocontrol zone and the chemical defense zone was counted, and the control effect (%) = (number of live insects before control-number of live insects after control) ×100/number of live insects before control, and the results are shown in table 1 and fig. 1.
TABLE 1 comparison of control effects of tea orange goiter from amblyseius pseudofront released from tea garden
Figure BDA0004176468530000041
Figure BDA0004176468530000051
As can be seen from Table 1 and FIG. 1, the saw-seiulus neoformans biological control garden before releasing less amblyseius is reduced by 6 times compared with the conventional chemical pesticide control garden, the pesticide usage amount is reduced by about 70%, the control effect on tea orange gall mites reaches more than 85% after releasing for 1 month, and the release duration for 1 time is longer than 8 months. And the amblyseius pseudopterus biological control garden released has better tea quality (figure 5 and figure 6) than the conventional chemical pesticide control garden, higher yield and obvious economic, social and ecological benefits.
Example 2
Biocontrol region: in a tea garden in the field county of Sanming city of Fujian province, a region with serious occurrence of 50 mu of relatively independent tea orange gall mites is selected as an experimental object, the tree age of the tea garden is 20 years, 5 points are adopted for sampling during investigation, 5 new shoots are selected from the east, the west, the south, the north and the middle of the tea garden respectively, the tea garden is brought back to a laboratory for observation and inspection under a microscope, 3 leaves are investigated from top buds to bottom every moment, 75 leaves are investigated in total, and the total quantity of all insect states of the tea orange gall mites is counted. The 6 months and 19 days are controlled by 15% pyridaben 1500 times liquid, 20 ten thousand saw-seiulus before amblyseius is released per mu in the 6 months and 26 days, and 20 ten thousand saw-seiulus before amblyseius is added into 2.5 kg of dried wheat bran, uniformly mixed and uniformly spread on the tea leaves of a tea garden. The investigation was done every 10 days after release. After release of amblyseius pseudopterogorus, no herbicide and chemical pesticide was used.
Chemical defense area: and a 10 mu conventional tea-proof garden is selected in the same place as a control, and the investigation method is the same as above. The chemical prevention garden is respectively controlled by 15% pyridaben 1500 times liquid for 6 months and 19 days, 73% propargite 1500 times liquid for 7 months and 30 days, 99% green (spraying) 150 times liquid for 9 months and 5 days, 15% quick-speed mite emulsifiable concentrate for 10 months and 5 days, 250 ml/mu and 15% pyridaben 1500 times liquid for 11 months and 15 days.
The control effect of the biocontrol zone and the chemical defense zone was counted, and the control effect (%) = (number of live insects before control-number of live insects after control) ×100/number of live insects before control, and the results are shown in table 2 and fig. 2.
TABLE 2 comparison of control effects of tea orange goiter from amblyseius pseudofront released from tea garden
Figure BDA0004176468530000061
Figure BDA0004176468530000071
As can be seen from Table 2 and FIG. 2, the release of the amblyseius pseudolaricis biological control garden reduces the use of chemical pesticides 4 times compared with the conventional chemical pesticide control garden, reduces the use amount of the pesticides by about 65%, achieves the control effect on tea orange gall mites by more than 85%, and has better tea quality, higher yield and remarkable economic, social and ecological benefits compared with the conventional chemical pesticide control garden.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.

Claims (9)

1. Application of amblyseius pseudopteropi as natural enemy in preventing and treating tea orange gall mite.
2. A method for preventing and controlling tea orange goiter mites is characterized in that saw seiulus less than hair is released in tea gardens 4-9 months each year.
3. The method of claim 2, wherein 15 to 20 tens of thousands of amblyseius pseudofront mites are released per mu.
4. The method of claim 2, wherein the release condition is: the average pest and mite amount in each leaf of the tea garden is lower than 2.
5. The method of claim 2, wherein the release of the amblyseius pseudopteran in the tea garden is submerged.
6. The method according to claim 5, wherein the submerged release method is: the wheat bran or the chaff is used for uniformly mixing the amblyseius pseudopteropi, and then uniformly spreading the mixed amblyseius pseudopteropi on the leaf surfaces of the tea trees.
7. The method of claim 5, wherein the submerged release time is: release in the morning and evening on sunny days or release in the whole cloudy day.
8. The method according to any one of claims 2 to 7, characterized in that the amblyseius pseudoplectania is not mixed with pesticides, fertilizers.
9. The method according to any one of claims 2 to 7, wherein after releasing amblyseius pseudoplectania, the tea garden is not used with herbicides or highly toxic chemical pesticides.
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WO2011160982A1 (en) * 2010-06-25 2011-12-29 Syngenta Participations Ag Methods for controlling mites
CN102283176A (en) * 2011-07-12 2011-12-21 黄建华 Artificial raising method for Amblyseius (Propriseiopsis) asetus
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