CN101743837A - Method for substituting for flaveria bidentis by utilizing combination of sunflower and perennial ryegrass - Google Patents
Method for substituting for flaveria bidentis by utilizing combination of sunflower and perennial ryegrass Download PDFInfo
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Abstract
The invention provides a method for substituting for flaveria bidentis by utilizing combination of sunflower and perennial ryegrass; in the method, the combination of the sunflower and the perennial ryegrass is adopted to biologically substitute for the flaveria bidentis; the germination rate of the flaveria bidentis can be reduced by 51.52 percent, the plant height can be reduced by 77.71 percent, the branching quantity of the single plant is reduced by 98.23 percent averagely, the leaf quantity of the single plant is reduced by 97.57 percent averagely, the biomass liveweight of the single plant is reduced by 97.87percent averagely, and the average soluble protein content is reduced by 73.5 percent, and the method has remarkable control function to external invasion of the flaveria bidentis; the seeds of the perennial ryegrass and the sunflower are obtained easily, and the planting method is simple and is easy to operate; in addition, the perennial ryegrass is a good pasture, the sunflower is an important oil crop; by adopting method, while controlling the quantity of the flaveria bidentis, a certain of economic value can be realized.
Description
Technical field
The present invention relates to the Ecological Control technical field, the biological substitution method of especially yellow top chrysanthemum.
Background technology
Biology invades another new environment by former existence ground warp approach nature or artificial, bio-diversity, agriculture, forestry, animal husbandry and fishery production and the human health of invading ground caused economic loss or ecocatastrophe, and (all places is great, Guo Jianying, Wang Dehui. the harm of Chinese exotic invasive biology and management game [J]. bio-diversity, 2002,10 (1): 119-125.), be biotic intrusion.At occurring in nature, biotic intrusion is a kind of ubiquitous phenomenon, and the kind of biotic intrusion has also comprised nearly all biological group, and along with global economic integration, international, domestic trade is come and gone more and more frequent, and the probability that biological success is invaded also increases greatly.The adventive invasion can produce serious disturbance and harm to structure, function and the ecotope of the specific ecosystem; Adventive invasion changes food chain by compacting or the mode of squeezing local species or the food network is formed and structure, particularly Alien Weeds, is often causing the diversity of flora to become very single on invasion ground, and is destroying the arable land; The adventive invasion has influence on each ecosystem and biota, makes hundreds of local species be absorbed in the extinction condition, has quickened the forfeiture of bio-diversity and the extinction of species; Adventive is invaded the bio-safety that can threaten a country, jeopardizes the ecological safety of a country, the economic security of a country of harm, and the people's interests of a country of infringement, even shake the stable foundation of a society.
China is that a real estate is abundant, the various large agricultural country of species, and biotic intrusion is particularly serious to the influence of China.Biological and the local species competition of exotic invasive living space, nutrient jeopardize the particularly existence of rare and endangered species of local species, cause the forfeiture of bio-diversity, to the 26S Proteasome Structure and Function generation harmful effect of China's ecosystem; The adventive invasion can bring direct economy harm to farmland gardening, lawn, forest, herding, aquatic products etc.; Thereby adventive is invaded by the natural ecosystems that change invasion ground, reduction species diversity local society, culture even health of people is produced serious harm.Biotic intrusion is just becoming one of key factor that threatens China's agriculture and forestry production, bio-diversity and ecotope.
At present, the identified exotic invasive biology of placing on record of China has 427 kinds, wherein: 55 kinds of plant pathogenic microorganismss (30 kinds of fungies, 6 kinds on bacterium, 10 kinds in virus, 9 kinds of nematodes); 244 kinds of plants (18 kinds of water plants, 226 kinds of Lu Sheng); 128 kinds of animals (comprising invertebrate, amphibious reptiles, fish, mammality etc.).Huang Dingju (Flaveria bidentis (L.) Kuntze) is to invade China in recent years, and agricultural is endangered very large exotic plant.
Huang Dingju belongs to composite family sneezeweed family yellow top Chrysanthemum, is annual herb, originates from South America, is that a kind of happiness light, happiness are wet, salt tolerant alkali, impoverishment tolerant, growth is rapid, fertility is strong, the great weeds of solid amount, has very strong resistance and environmental suitability.Huang Dingju propagates into states such as South Africa, Egypt, Britain, France, Australia, Japan owing to reason such as introducing a fine variety, and pernicious invasive weed becomes international.Chrysanthemum can be invaded multiple ecotope on yellow top, invasion ground such as comprises before the edge of a field, irrigation canals and ditches limit, limit, rivers and lakes, Rail Highway limit, the room behind the house, in case just can form single advantage group, repel local species, thoroughly change species composition, quantity and the trophic structure of the ecosystem.Behind the Huang Dingju invasion farmland, strive fertilizer with crops, strive water, win honour for, suppress crop growth.Behind the chrysanthemum of the yellow top of wheatland growth, can cause a mu spike number reduction, thousand kernel weight to descend.In the soil of yellow top chrysanthemum of growing, plant soybean, and its germinating capacity reduction (Zhou Xianzhong, Liu Liyun. brief talk harm and the control [J] of the yellow top of new alien species chrysanthemum. agricultural technology service, 2008,25 (1): 52-89.).Yellow top chrysanthemum aggressiveness and resistance are extremely strong, and vitality is very vigorous, by constantly striving unexpectedly, substitute the eciophyte resource, and the ecosystem of invading ground is caused irreversible destruction, cause serious biological pollution.The florescence of Huang Dingju is long, and pollen amount is big, florescence and most of indigenous composite family juxtaposition.If other the indigenous feverfew intergeneric cross in Huang Dingju and the generation area just might cause forming the bigger species of new harmfulness.
From calendar year 2001 since lakeside, Hengshui, China Hebei province is found first, owing to lack effective prevention and control technical measures, Huang Dingju in Hebei province, Tianjin, Shandong Province and Henan Province's diffusion spread, and causes large tracts of land harm, China's grain-production and ecotope are constituted a serious threat.Only 2007, the Huang Ding of Hebei province chrysanthemum epidemic situation expanded 11 districts and cities, 89 counties (district, city) to, and the serious harm area reaches more than 560,000 mu.
Chrysanthemum can become invasive species gradually along with the expansion of range of scatter on yellow top, the root system that one of its reason is exactly yellow top chrysanthemum can be secreted allelochemical (plant or microorganism secretion a kind of to other plant in the environment or the favourable or disadvantageous metabolic secretion thing of microorganism) and suppress other plant growth (Li Xiangju, Zhang Miru, Li Yongjun, Deng. yellow top chrysanthemum aqueous extract is to the STUDY ON ALLELOPATHIC EFFECTS [J] of plant seed germination and radicle elongation. the weeds science, 2007, (4): 15-19.).At present, to the allelochemical among Huang Dingju research with report more flavonoids (Quercetin sulfuric ester) (the Guglielmone H A that is mainly, AgneseA M, Nunez Montoya S C, et al.Anticoagulant effect and action mechanismof sulphated flavonoids from Flaveria bidents[J] .Thrombosis Research, 2002,105 (2): 183-188.) and thiophenes (Agnese A M, Nunez Montoya S C, Espinar L A, et al.Chemotaxonomic features in Argentinian species[J] .Biochemical Systematics and Ecology, 1999,27:739-742.).Domestic there are some researches show, the flower of Huang Dingju, root, stem, leaf extract all have allelopathy to three-coloured amaranth, green foxtail, lamb's-quarters seed germination, and it is relevant with extract concentration, show the extract of Huang Dingju can reduce the length of seed germination rate, radicle (Li Sujing. yellow top chrysanthemum study on allelopathy [J]. the Shaanxi agricultural science, 2007, (6): 80-81.).The different extracts of yellow top chrysanthemum show in various degree inhibitory action to wheat seedling growth, the active concentration along with the each several part extract of this inhibition increases and strengthens, under the same concentrations new cadmium yellow top chrysanthemum plant water extract to the inhibitory action of wheat seedling growth be better than its root system water extract to the inhibitory action of wheat seedling growth (Chen Yan. the yellow top of exotic invasive weed chrysanthemum is analyzed .2008, Master's thesis to the allelopathy of wheat .).Yellow top chrysanthemum cauline leaf water extract all shows in various degree inhibitory action to corn, wheat, cotton, soybean, peanut, lady's-grass and Amaranthus retroflexus, wherein the strongest with change sense effect to cotton, when its concentration is 0.2g/mL, change sense index long to root and that stem is long be respectively-0.85 with-0.88 (Xu Wenchao, Xu Jiao makes pottery and feeds, etc. the stranger invades the allelopathy Primary Study [J] of the yellow top of plant chrysanthemum. Agricultural University Of Hebei's journal, 2007,30 (6): 63-67.).
In addition, yellow top chrysanthemum root system is very flourishing, and water absorbing capacity is extremely strong, and growth potential is strong.Its plant is tall and big, and branches and leaves are very dense, seriously blocked the necessary sunlight of other biological growth, tie up the living space of other plant, have ecological occupancy, and it is single to form vegetation in the course of time, the abominable ecotope of plant resources scarcity.
The main method of administering yellow top chrysanthemum at present has:
Physical control: one of the most effective the method for control at present, be Huang Dingju vegetative growth phase 4～August, also is the best opportunity of rooting out Huang Dingju, fully mobilize the masses, to generation in blocks area, elder generation's manpower cuts off plant, ploughs the solarization root again, chooses most root stubble again, burn then, accomplish to pull the grass up by its roots, with discovery, with pulling out.But physical control consumes lot of manpower and material resources, and efficient is low, and is serious for harm, that area is big is local inapplicable.
Chemical control: utilizing chemical control is one of main method of the yellow top of control chrysanthemum, steriland herbicide glyphosate, paraquat have effect preferably to the anti-top chrysanthemum of harnessing the Yellow River, hormone herbicide has special efficacy (Wang Qiuxia to the anti-top chrysanthemum of harnessing the Yellow River, Zhang Hongjun, Guo Meixia, Deng. the chemical control [J] of the yellow top of exotic invasive weed chrysanthemum. ecotope, 2008,17 (3): 1184-1189.).But chemical control can not be eradicated Huang Dingju to the seed weak effect in the soil, and concerning human health and non-target species too many threat is arranged all.In addition, along with a large amount of uses of chemical agent, the resistance of Huang Dingju will constantly increase, and the price of medicament costliness and the necessity of repetitive administration often make continuous dispenser control become unlikely.
Biological control: utilize natural enemy animal or pathogenic microorganisms prevent harnessing the Yellow River the top chrysanthemum still under test, can't thoroughly root out Huang Dingju effectively.
Summary of the invention
In order to address the above problem, the invention provides the exotic invasive that a kind of biological substitution method is controlled yellow top chrysanthemum, technical scheme of the present invention is as follows:
1, a kind of biological substitution method of controlling yellow top chrysanthemum exotic invasive is characterized in that, described method adopts sunflower (Helianthus annuus) and English ryegrass (Lolium perenne L.) combination that Huang Dingju is carried out biological substitution.
2, as technical scheme 1 described biological substitution method, wherein, described English ryegrass and sunflower are adopted the seeding method plantation.
3, as technical scheme 2 described biological substitution methods, wherein, described English ryegrass adopts the mode of drilling to plant.
4, as technical scheme 3 described biological substitution methods, wherein, the plantation line-spacing of described English ryegrass is 20cm～30cm.
5, as technical scheme 2 described biological substitution methods, wherein, described sunflower adopts the mode of program request to plant.
6, as technical scheme 2～5 each described biological substitution methods, wherein, described sunflower planting in described English ryegrass plantation in the ranks.
7, as technical scheme 6 described biological substitution methods, wherein, the plantation spacing in the rows of described sunflower is 30cm～50cm.
8, as technical scheme 2～7 each described biological substitution methods, wherein, the sowing depth of described English ryegrass is 1cm～3cm.
9, as technical scheme 2～8 each described biological substitution methods, wherein, the sowing depth of described sunflower is 5cm～10cm.
10, as technical scheme 2～9 each described biological substitution methods, wherein, the planting density of described English ryegrass is 1g/m
2～3g/m
2
11, as technical scheme 2～10 each described biological substitution methods, wherein, the planting density of described sunflower is 2/m
2～4/m
2
The biological substitution method of the yellow top of control provided by the invention chrysanthemum exotic invasive has the following advantages: the yellow top of the combination replacement chrysanthemum of adopting English ryegrass and sunflower, can make the emergence rate of Huang Dingju reduce 51.52%, plant height reduces by 77.71%, average individual plant divides number to reduce 98.23%, average single-strain blade number reduces 97.57%, average biomass of individual tree reduces 97.87%, average soluble protein content reduces 73.5%, and the inventive method can play significant control action to the exotic invasive of Huang Dingju.The seed of English ryegrass and sunflower obtains easily, and the kind method for planting is simple, easy to operate, can exempt a large amount of manpower and materials that physical control consumes; English ryegrass and sunflower are frequently seen plants, can exempt the threat of chemical control to human health and non-target species; English ryegrass is a kind of herbage of high-quality, and sunflower is a kind of important oil crop, can realize that again certain economic is worth in the chrysanthemum population quantity of the yellow top of control.
Embodiment
As mentioned before, the invention provides a kind of biological substitution method of controlling yellow top chrysanthemum exotic invasive, described method adopts English ryegrass and sunflower to come Huang Dingju is carried out biological substitution.
In the present invention, term " biological substitution method " is meant by planting one or more plants, another or growing of various plants in the same growing environment are suppressed, thereby make described " one or more plants " substitute the method for described " another or various plants " in described same growing environment, this biological substitution is owned by France in a kind of biological control method at replaced plant.In the present invention, replaced plant is Huang Dingju, is English ryegrass and sunflower in order to the plant that substitutes yellow top chrysanthemum.
With regard to perennial ryegrass, although it also is not very clear at present about it Huang Dingju being had the concrete reason that significant biological substitution acts on the microcosmic point, but the inventor thinks it may is because under field conditions (factors), the perennial ryegrass seedling-growing time is more Zao than yellow top chrysanthemum, and its fibrous root prosperity, tillering ability are strong, thereby planting speed is fast, can cover ground rapidly, thereby suppresses yellow top chrysanthemum growth of seedlings.
With regard to sunflower, although it also is not very clear at present about it Huang Dingju being had the concrete reason that significant biological substitution acts on the microcosmic point, but the inventor thinks it may is because sunflower drought resisting strong stress resistance, environmental suitability is strong, the sunflower late growing stage is rapid, can in certain zone, formation win honour for Huang Dingju, thereby suppress yellow growth of pushing up chrysanthemum than big crown density.
The kind method for planting general using type of seeding of English ryegrass and sunflower is carried out, the inventive method has no particular limits the seeding method of English ryegrass and sunflower, can adopt for example drilling, program request or broadcast sowing etc., but from the chrysanthemum growth of the yellow top of more abundant inhibition be convenient to gather in the crops aspects such as English ryegrass and sunflower and take all factors into consideration, the preferred bar broadcast mode that adopts is planted English ryegrass, adopts a some broadcast mode plantation sunflower.
The inventive method was not particularly limited the concrete time of the sowing of English ryegrass and sunflower, the growth as long as suitable English ryegrass of weather conditions and sunflower emerge.For example, in the area, Hebei, the suitable sowing time of English ryegrass is mid or late April, the seedling-growing time of Huang Dingju is generally late April, the perennial ryegrass seedling-growing time is more Zao than yellow top chrysanthemum, and its fibrous root prosperity, tillering ability are strong, thereby planting speed is fast, can cover ground rapidly, thereby suppress yellow top chrysanthemum growth of seedlings; The suitable sowing time of sunflower is early or mid May, and the sunflower late growing stage is rapid, can formation win honour for Huang Dingju than big crown density in certain zone, thereby form the inhibition that Huang top chrysanthemum is grown.In an embodiment of the present invention, for the ease of operation, sunflower is also planted in mid or late April, because implantation time is too early, it is not fine causing sunflower late growing stage situation, but nonetheless, and English ryegrass and sunflower combination are to the inhibitory action of Huang Dingju still clearly, as seen, the combination of English ryegrass and sunflower has very strong biological substitution effect to Huang Dingju.
Under the situation of drilling English ryegrass, the inventive method has no particular limits the plantation line-spacing.But, from the chrysanthemum growth of the yellow top of more abundant inhibition, be convenient to gather in the crops English ryegrass and gather in the crops aspect such as more English ryegrass and consider that drill spacing is preferably 20cm～30cm, as being 20cm, 22cm, 24cm, 26cm, 28cm or 30cm.If line-spacing is excessive, then may be unfavorable for the growth of Huang Dingju is effectively suppressed; If line-spacing is too small, then may be unfavorable for the growth of English ryegrass self.
Under the situation of program request sunflower, the plantation that sunflower point is sowed at English ryegrass in the ranks, the inventive method has no particular limits the sunflower planting spacing in the rows.But, from the chrysanthemum growth of the yellow top of more abundant inhibition, be convenient to gather in the crops sunflower and gather in the crops aspect such as more sunflower and consider that the program request spacing in the rows is preferably 30cm～50cm, as being 30cm, 35cm, 40cm, 45cm or 50cm.If spacing in the rows is excessive, then possibly can't realizes good crown density, thereby can't the growth of Huang Dingju effectively be suppressed; If spacing in the rows is too small, then may be unfavorable for the growth of sunflower self.
In addition, the sowing depth of English ryegrass is generally 1cm～3cm, as being 1cm, 1.5cm, 2cm, 2.5cm or 3cm; The sowing depth of sunflower is generally 5cm～10cm, as being 5cm, 6cm, 7cm, 8cm, 9cm or 10cm.If sowing depth is dark excessively, then may be unfavorable for being unearthed of English ryegrass and sunflower; If sowing depth is shallow excessively, then may be unfavorable for the fixing of English ryegrass and sunflower root system, thereby influences the late growing stage of English ryegrass and sunflower.
In addition, the planting density of English ryegrass is generally 1g/m
2～3g/m
2, for example can be 1g/m
2, 1.5g/m
2, 2g/m
2, 2.5g/m
2Or 3g/m
2The planting density of sunflower is generally 2/m
2～4/m
2, as being 2/m
2, 3/m
2Or 4/m
2If planting density is too small, then may be unfavorable for the growth of Huang Dingju is effectively suppressed; If planting density is excessive, then may be unfavorable for the growth of English ryegrass and sunflower self.
For the maintenance in later stage, the present invention is not particularly limited, and can adopt conventional field method that English ryegrass and sunflower are carried out maintenance management.
Incidentally, except as otherwise noted, otherwise the number range that reaches described herein comprises the arbitrary value between end value and the endpoints thereof (being upper and lower bound).
Hereinafter will come the present invention is further detailed, but these embodiment should not be construed as limitation of the scope of the invention only for the purpose of illustration by embodiment.
Embodiment
Embodiment 1
April 18, in chrysanthemum spot, Huang top, adopt the mode of drilling to plant English ryegrass, the plantation line-spacing is 20cm, and adopts the mode of program request to plant sunflower in the ranks English ryegrass simultaneously, spacing in the rows is 30cm.The sowing depth of English ryegrass is 1cm, and the sowing depth of sunflower is 5cm; The planting density of English ryegrass is 1g/m
2, the planting density of sunflower is 2/m
2Adopt conventional field method that English ryegrass and sunflower are carried out maintenance management.Following data are measured when Huang Dingju is bloomed, the emergence rate that draws Huang Dingju is 52.9%, average plant height is 72.82cm, and it is that 17.7, average single-strain blade number are that 120, average biomass of individual tree are that 13.37g, average soluble protein content are 0.45mgg that average individual plant divides number
-1FW, average protective enzyme content is 10.06 Δ A470min
-1G
-1FW.
Embodiment 2～12
Except that listed content in the table 1, embodiment 2～12 adopts the mode identical with embodiment 1 to implement.
Comparative example
Do not sow English ryegrass and sunflower in chrysanthemum spot, Huang top, following data are measured when Huang Dingju is bloomed, the emergence rate that draws Huang Dingju is 92%, average plant height is 185cm, and it is that 575, average single-strain blade number are that 1850, average biomass of individual tree are that 335g, average soluble protein content are 1.17mgg that average individual plant divides number
-1FW, average protective enzyme content is 11.5 Δ A470min
-1G
-1FW.
Every physical signs of the Huang Dingju that each embodiment and comparative example are measured is as shown in table 2.
The planting patterns that each embodiment of table 1 is adopted
The parameters of the Huang Dingju that each embodiment of table 2 and comparative example are measured
| Numbering | Emergence rate (%) | Average plant height (cm) | Average individual plant divides number (individual) | Average single-strain blade number (sheet) | Average biomass of individual tree (g) | Average soluble protein content (mgg -1??FW) | Average protective enzyme content (Δ A470min -1??·g -1FW) |
| Embodiment 1 | ??52.9 | ??72.82 | ??17.7 | ??120 | ??13.37 | ??0.45 | ??10.06 |
| Embodiment 2 | ??48.7 | ??59.62 | ??15.7 | ??95 | ??10.59 | ??0.39 | ??12.19 |
| Embodiment 3 | ??44.6 | ??41.23 | ??10.2 | ??45 | ??7.12 | ??0.31 | ??17.06 |
| Embodiment 4 | ??48.5 | ??60.18 | ??15.9 | ??101 | ??10.97 | ??0.28 | ??12.43 |
| Embodiment 5 | ??43.9 | ??46.59 | ??9.6 | ??65 | ??8.16 | ??0.37 | ??16.89 |
| Embodiment 6 | ??47.2 | ??53.28 | ??12.7 | ??81 | ??9.21 | ??0.41 | ??14.54 |
| Embodiment 7 | ??51.3 | ??65.13 | ??16.9 | ??116 | ??12.61 | ??0.44 | ??10.89 |
| Embodiment 8 | ??47.5 | ??54.26 | ??12.1 | ??83 | ??9.37 | ??0.43 | ??14.27 |
| Embodiment 9 | ??43.2 | ??38.53 | ??8.1 | ??30 | ??6.43 | ??0.23 | ??17.58 |
| Embodiment 10 | ??48.1 | ??52.37 | ??13.5 | ??87 | ??10.01 | ??0.41 | ??13.69 |
| Embodiment 11 | ??49.2 | ??64.79 | ??16.2 | ??109 | ??11.83 | ??0.37 | ??11.92 |
| Numbering | Emergence rate (%) | Average plant height (cm) | Average individual plant divides number (individual) | Average single-strain blade number (sheet) | Average biomass of individual tree (g) | Average soluble protein content (mgg -1??FW) | Average protective enzyme content (Δ A470min -1??·g -1FW) |
| Embodiment 12 | ??45.7 | ??51.34 | ??10.4 | ??72 | ??8.84 | ??0.34 | ??15.71 |
| Comparative example | ??92 | ??185 | ??575 | ??1850 | ??335 | ??1.17 | ??11.5 |
Annotate: average biomass of individual tree is meant the average biomass of individual tree of yellow top chrysanthemum acrial part.
The assay method of each index of Huang Dingju is following, and (each sub-district is 3 * 3m
2, picked at random):
Emergence rate: according to the thousand kernel weight of seed, the sowing grain number of calculation plot, count the number of emerging of respective cell the back of emerging, and asks the mean value of calculating emergence rate.
Average plant height: 5 samplings in every sub-district, get 4 strains at every, be the tape measure mensuration plant height of 1mm with minimum scale, calculate the mean value of plant height.
Average individual plant divides all single-strain blade numbers of number peace: 5 samplings in each sub-district, and get 4 strains at every and count and ask calculation mean value.
Average biomass of individual tree: 5 samplings in each sub-district, every acrial part of getting 4 strains, with baking oven 104 ℃ complete 15 clocks after, dry to constant weight at 80 ℃.On electronic balance, claim its dry weight.
Average soluble protein content: 5 samplings in each sub-district, get 4 strains at every, three leaf a slices of plant are got in every strain, take by weighing 0.3g～0.5g after the mixing, shred and in the mortar of freezing mistake, add extract 3ml, add a small amount of quartz sand, in ice bath, grind to form homogenate fast, homogenate is poured in the centrifuge tube, use 5ml extract (at twice) that homogenate in the mortar is washed centrifuge tube again, then 10000r/min (rev/min), centrifugal 20min under 4 ℃ of conditions (minute), supernatant is the soluble protein extract.The Coomassie brilliant blue method of employing Gisnnopolitis (Gisnnopolitis C, Nries S K.Superoxide dismutases occurrence in higher plants.Plant Physiology, 1977,59:309-314.) measure.Get 0.1ml enzyme liquid and put into test tube respectively, add Tris buffer solution 0.9ml in the test tube again, the blank pipe adds Tris buffer solution 1ml, and each pipe adds coomassie brilliant blue staining liquid 5ml respectively more then, shakes up, and measures absorbance (A) value at 595nm wavelength place.Each sample repeats 3 times.
Average protective enzyme content (POD): 5 samplings in each sub-district, get 4 strains at every, take by weighing 0.5g left and right sides sample; adding 5mL extraction buffer solution (including 1% crospovidone) is extract, and ice bath grinds, and changes centrifuge tube then over to; under 4 ℃, 10000r/min, centrifugal 10min.Supernatant is a crude enzyme liquid.The guaiacol method of employing Li Hesheng (the Li Hesheng chief editor, plant physiology and biochemistry experimental principle and technology, higher education publishing house, Beijing: 2000) measure, the absorbance of working sample reaction 3min under the 470nm wavelength, every 30s (second) reads a secondary data.Each sample repeats 3 times.
Result from last table 2 as can be seen, the combination of English ryegrass and sunflower is remarkable to the biological substitution effect of Huang Dingju.The inventor finds can further strengthen the inhibitory action to Huang Dingju by optimizing the planting density of English ryegrass or sunflower in further testing.As can be seen from Table 2, adopt the yellow top of the combination replacement chrysanthemum of English ryegrass and sunflower, can make the emergence rate of Huang Dingju reduce 51.52%, plant height reduces by 77.71%, average individual plant divides number to reduce 98.23%, average single-strain blade number reduces 97.57%, and average biomass of individual tree reduces 97.87%, and average soluble protein content reduces 73.5% (calculate according to the data of embodiment 3 and comparative example and get).And, along with the increase of the planting density of English ryegrass or sunflower, the emergence rate of Huang Dingju, average plant height, average individual plant divide indexs such as number, average single-strain blade number, average biomass of individual tree, average soluble protein content that the trend that reduces is all arranged.But increase along with the planting density of English ryegrass or sunflower; the content of average protective enzyme has the trend of increase; this may be because the combination of English ryegrass and sunflower has bigger coercive to Huang Dingju; thereby stimulate the more protective enzyme of generation, so the content of average protective enzyme has the trend of increase.
Embodiment described herein only is to the explanation of the preferred embodiments of the present invention example.The technical staff of the technical field of the invention can content disclosed according to the present invention carry out various modifications to described embodiment, but revises thus and the technical scheme that does not depart from essence of the present invention that obtains still drops within the appended claims institute restricted portion.
Claims (10)
1. a biological substitution method of controlling yellow top chrysanthemum exotic invasive is characterized in that, described method employing sunflower and English ryegrass make up Huang Dingju is carried out biological substitution.
2. biological substitution method as claimed in claim 1, wherein, described English ryegrass and sunflower are adopted the seeding method plantation.
3. biological substitution method as claimed in claim 2, wherein, described English ryegrass adopts the mode of drilling to plant.
4. biological substitution method as claimed in claim 3, wherein, the plantation line-spacing of described English ryegrass is 20cm～30cm.
5. biological substitution method as claimed in claim 2, wherein, described sunflower adopts the mode of program request to plant.
6. as each described biological substitution method of claim 2～5, wherein, described sunflower planting is planted in the ranks in described English ryegrass, and spacing in the rows is 30cm～50cm.
7. as each described biological substitution method of claim 2～6, wherein, the sowing depth of described English ryegrass is 1cm～3cm.
8. as each described biological substitution method of claim 2～7, wherein, the sowing depth of described sunflower is 5cm～10cm.
9. as each described biological substitution method of claim 2～8, wherein, the planting density of described English ryegrass is 1g/m
2～3g/m
2
10. as each described biological substitution method of claim 2～9, wherein, the planting density of described sunflower is 2/m
2～4/m
2
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| CN200910265408A Pending CN101743837A (en) | 2009-12-28 | 2009-12-28 | Method for substituting for flaveria bidentis by utilizing combination of sunflower and perennial ryegrass |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102090258A (en) * | 2010-11-25 | 2011-06-15 | 中国农业科学院农业环境与可持续发展研究所 | Method for replacing and controlling flaveria bidentis (L.) kuntze with astragalus adsurgens |
| CN102823466A (en) * | 2012-09-11 | 2012-12-19 | 中国农业科学院农业环境与可持续发展研究所 | Method for replacing and controlling cenchrus paciflorus by sunflower |
| CN105432303A (en) * | 2015-11-27 | 2016-03-30 | 何军 | Autumn-sowing stable-yield cultivation method for sunflowers in the south |
| CN108419637A (en) * | 2018-01-29 | 2018-08-21 | 河北大学 | A kind of biological substitution method of control bidentis exotic invasive |
| CN109874612A (en) * | 2019-03-05 | 2019-06-14 | 江苏大学 | A kind of biological substitution method controlling pernicious invasive weed Solidago Canadensis |
| CN112997827A (en) * | 2021-02-21 | 2021-06-22 | 云南省农业科学院农业环境资源研究所 | Method for replacing and controlling mikania micrantha by sweet potato and hyacinth bean combination |
| CN113924930A (en) * | 2021-01-18 | 2022-01-14 | 云南省农业科学院农业环境资源研究所 | Method for replacing and controlling exotic invasive plant mikania micrantha in sloping farmland by utilizing species combination of sweet potatoes and ryegrass |
-
2009
- 2009-12-28 CN CN200910265408A patent/CN101743837A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102090258A (en) * | 2010-11-25 | 2011-06-15 | 中国农业科学院农业环境与可持续发展研究所 | Method for replacing and controlling flaveria bidentis (L.) kuntze with astragalus adsurgens |
| CN102090258B (en) * | 2010-11-25 | 2012-04-25 | 中国农业科学院农业环境与可持续发展研究所 | Method for replacing and controlling flaveria bidentis (L.) kuntze with astragalus adsurgens |
| CN102823466A (en) * | 2012-09-11 | 2012-12-19 | 中国农业科学院农业环境与可持续发展研究所 | Method for replacing and controlling cenchrus paciflorus by sunflower |
| CN105432303A (en) * | 2015-11-27 | 2016-03-30 | 何军 | Autumn-sowing stable-yield cultivation method for sunflowers in the south |
| CN108419637A (en) * | 2018-01-29 | 2018-08-21 | 河北大学 | A kind of biological substitution method of control bidentis exotic invasive |
| CN108419637B (en) * | 2018-01-29 | 2021-08-10 | 河北大学 | Biological substitution method for controlling external invasion of flaveria bidentis |
| CN109874612A (en) * | 2019-03-05 | 2019-06-14 | 江苏大学 | A kind of biological substitution method controlling pernicious invasive weed Solidago Canadensis |
| CN113924930A (en) * | 2021-01-18 | 2022-01-14 | 云南省农业科学院农业环境资源研究所 | Method for replacing and controlling exotic invasive plant mikania micrantha in sloping farmland by utilizing species combination of sweet potatoes and ryegrass |
| CN113924930B (en) * | 2021-01-18 | 2023-01-31 | 云南省农业科学院农业环境资源研究所 | Method for replacing and controlling exotic invasive plant mikania micrantha in sloping farmland by utilizing species combination of sweet potatoes and ryegrass |
| CN112997827A (en) * | 2021-02-21 | 2021-06-22 | 云南省农业科学院农业环境资源研究所 | Method for replacing and controlling mikania micrantha by sweet potato and hyacinth bean combination |
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