CN102532342B - Crude polysaccharide extract of mokara root and application - Google Patents
Crude polysaccharide extract of mokara root and application Download PDFInfo
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- CN102532342B CN102532342B CN2012100858333A CN201210085833A CN102532342B CN 102532342 B CN102532342 B CN 102532342B CN 2012100858333 A CN2012100858333 A CN 2012100858333A CN 201210085833 A CN201210085833 A CN 201210085833A CN 102532342 B CN102532342 B CN 102532342B
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 52
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 52
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 52
- 239000000284 extract Substances 0.000 title claims abstract description 25
- 241001184535 x Mokara Species 0.000 title claims abstract description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 48
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 31
- 239000006228 supernatant Substances 0.000 claims abstract description 15
- 239000012153 distilled water Substances 0.000 claims abstract description 12
- 239000000243 solution Substances 0.000 claims abstract description 10
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- 235000019441 ethanol Nutrition 0.000 claims description 14
- 238000010521 absorption reaction Methods 0.000 claims description 13
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- 238000003756 stirring Methods 0.000 claims description 7
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 2
- 239000012467 final product Substances 0.000 claims description 2
- 238000003809 water extraction Methods 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 abstract description 6
- 230000007613 environmental effect Effects 0.000 abstract description 2
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- MEIRRNXMZYDVDW-MQQKCMAXSA-N (2E,4E)-2,4-hexadien-1-ol Chemical compound C\C=C\C=C\CO MEIRRNXMZYDVDW-MQQKCMAXSA-N 0.000 description 1
- AXDJCCTWPBKUKL-UHFFFAOYSA-N 4-[(4-aminophenyl)-(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]aniline;hydron;chloride Chemical compound Cl.C1=CC(=N)C(C)=CC1=C(C=1C=CC(N)=CC=1)C1=CC=C(N)C=C1 AXDJCCTWPBKUKL-UHFFFAOYSA-N 0.000 description 1
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- Polysaccharides And Polysaccharide Derivatives (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a crude polysaccharide extract of a mokara root, which is obtained by the following steps: placing the mokara root in distilled water, extracting for 1-2h under the condition of ultrasound at the temperature of 50-60 DEG C, filtering, filtering residue, extracting for twice, and blending the filtrate for three times; carrying out pressure-reducing concentration to the filtrate, adding ethanol solution to the concentrated solution until ethanol in the mixed solution accounts for 80% by volume, fully and evenly mixing, and refrigerating for 12-24h at the temperature of 4 DEG C; removing supernatant of the refrigerated mixture, precipitating, taking a precipitate after centrifugation, adding distilled water to perform dissolution, standing, taking the supernatant after centrifugation, adding ethanol solution, taking the precipitate after centrifugation, adding distilled water to perform dissolution, adding absolute ethanol, taking the supernatant after centrifugation, and obtaining the crude polysaccharide extract after freeze drying. The crude polysaccharide extract of the mokara root is all natural plant polysaccharide, and not only has the excellent moisture-preserving and moisture-absorbing performance, but also meets the requirements of users in the aspects of safety and environmental protection.
Description
Technical field
The invention belongs to technical field of plant extraction, be specifically related to a kind of crude polysaccharide extract of mokara root and application.
Background technology
Moisturizing research is the focus of cosmetic conduct and learning, dermatology.The effect of wetting Agent for Printing Inks in makeup is the water-content of keeping or increase keratoderma, improve the xerosis cutis that causes because of the stratum corneum lack of water and consequently itch, the clinical symptom such as furfur.Wetting Agent for Printing Inks can be divided into natural moisture preserving agent and synthetic wetting Agent for Printing Inks by its source, can be divided into again general wetting Agent for Printing Inks, softener, deep moisturizing agent by its mechanism of action.The wetting Agent for Printing Inks that uses in makeup at present is mainly polyvalent alcohol, sorbyl alcohol, lactic acid and sodium salt thereof etc.The natural moisture preserving agent with nutrition and moisturizing dual property of extracting from crude substance meets the trend of following wetting Agent for Printing Inks development.
Polysaccharide is to be present in the polymkeric substance that natural aldose or ketose link together by glycosidic link.Wide at the occurring in nature distributed pole, be prevalent in plant, animal, microorganism and marine alga, mainly contain vegetable polysaccharides, animal polysaccharide, microbial polysaccharide, Sargassum polysaccharides etc.Extract in recent years the focus that the natural moisture preserving agent with nutrition and moisturizing dual-use function is research from crude substance, natural polysaccharide is the rare material which can retain moisture of a class.Polysaccharide is applied to makeup as wetting Agent for Printing Inks. satisfied the requirement of natural, nutrition that people propose makeup, nonirritant etc.
The Mohs orchid (
Mokara) be the orchid family herbaceous plant, be 1969 by the Singaporean with blue, the bird tongue blue, three genus of Hymenocallis americana hybridize out novel orchid all ages.This is a class happiness light, and growing way is strong, and output is high, and spray is long, and pattern is gorgeous, is well suited for doing the orchid of cut-flower, and has certain pharmaceutical use.The blue main root that leans on of Mohs that has broken away from soil absorbs moisture and survives from air, the Mohs orchid has luxuriant aerial root, and fast growth can reach several meters.Be to increase yield and quality, the flower-grower need to be aging to these, unnecessary aerial root is regularly pruned, thereby produced a large amount of discarded roots.
Find through inspection information, orchid is not only precious ornamental plant, but also is important Source of Drug Plants.According to the Compendium of Material Medica record, " fragrant thoroughwort, smell is hot, flat, sweet, nontoxic.Its gas delicate fragrance, promote the production of body fluid to quench thirst, profit muscle is controlled the ehat in the gallbladder channel of quenching one's thirst ".According to reported in literature, mainly contain the compounds such as polysaccharide, phenols, terpene, flavones in orchid.The Mohs orchid is the novel cymbidium variety of hybridization, and it is formed and the research of effect yet there are no reported in literature, and extraction and the moisturizing activity research of carrying out Mohs orchid root polysaccharide will be a very significant job.Using ultrasound ripple method is extracted the Crude polysaccharides of Mohs orchid root, and the performance of keeping humidity of commonly using wetting Agent for Printing Inks glycerine in the Crude polysaccharides that extracts and makeup is compared research, explore its performance of keeping humidity, thereby being turned waste into wealth, the Mohs orchid root is applied to the production of makeup, for the tropical Mohs orchid of comprehensive development and utilization provides scientific basis, for the development orchid industry provides new thinking.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of in a kind of crude polysaccharide extract of mokara root is provided.
The technical problem that the present invention also will solve is to provide uses tropical Mohs orchid root Crude polysaccharides, can moisture absorption and the effect of moisturizing.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
A kind of crude polysaccharide extract of mokara root, it is prepared by following steps:
(1) the blue root of Mohs is placed in distilled water, extracts 1 ~ 2h under 50 ~ 60 ℃, ultrasound condition, filter, filter residue repeats to merge 3 times filtrate with distilled water extraction 2 times;
(2) filtrate that step (1) is obtained is at 70 ~ 80 ℃ of lower concentrating under reduced pressure, adds in the concentrated solution ethanolic soln volumn concentration of ethanol to the mixed solution to reach 80%, fully after mixing, refrigerates 12 ~ 24 h under 4 ℃;
(3) remove the supernatant liquor of the mixture after step (2) refrigerates, remaining precipitation is got precipitation after centrifugal, the adding distil water dissolving, standing, then get supernatant liquor after centrifugal, add ethanolic soln to stir, get precipitation after centrifugal, the adding distil water dissolving, then add absolute ethyl alcohol and stirring even, get precipitation after centrifugal, through lyophilize and get final product.
In step (1), the blue root of described Mohs is the blue root of the Mohs after shredding.
In step (1), for the blue root of every g Mohs, the volumetric usage of distilled water is 10 ~ 40mL.
In step (1), ultrasound condition is ultrasonic frequency 45kHz, and the ultrasonic power ratio is 70 ~ 80%.
In step (2) or (3), described volumes of aqueous ethanol percentage concentration is 90 ~ 95%.
In step (3), the volume that adds ethanolic soln is 4 ~ 8 times of supernatant liquor volume to be added.
In step (3), the volume that adds of dehydrated alcohol is 4 ~ 8 times of system volume to be added.
In step (3), centrifugal condition is 3000 r/min, 10 ~ 15 min.
The application of above-mentioned crude polysaccharide extract of mokara root in moisturizing, moisture absorption field.
The application of above-mentioned crude polysaccharide extract of mokara root in moisture-keeping cosmetics or medicine.
The application of above-mentioned crude polysaccharide extract of mokara root in moisture absorption makeup or medicine.
Beneficial effect: crude polysaccharide extract of mokara root of the present invention is the vegetable polysaccharides of pure natural, and it not only has good moisturizing, hygroscopic property, and has satisfied the demand of people aspect security, environmental protection.Technology of the present invention provides reference for the exploitation of the blue root Crude polysaccharides of Mohs.
Description of drawings
Different sample rate of moisture absorption and time chart when Fig. 1 RH is 43%.
Different sample rate of moisture absorption and time chart when Fig. 2 RH is 81%.
Fig. 3 is sample percentage of water loss and time chart.
Embodiment
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand, the described content of embodiment only is used for explanation the present invention, and should also can not limit the present invention described in detail in claims.
Embodiment 1: the blue root Crude polysaccharides of preparation Mohs.
100.0g is cleaned, oven dry, the Mohs orchid root of chopping, ultrasonic with 60 ℃ of 2000ml distilled water, ultrasonic frequency 45kHz, the ultrasonic power ratio is 80%, heating 1h, filter, filter residue repeats to extract 2 times, merge 3 filtrates and be evaporated to 100ml under 80 ℃, concentrated solution adds 95% (v/v) ethanol of 5 times of volumes, make the ethanol volumn concentration reach 80%, fully after mixing, 4 ℃ of refrigerator cold-storage 12 h, then remove supernatant liquor, to precipitate centrifugal (3000 r/min, 10 min) after, get the dissolving of precipitation adding distil water, standing, centrifugal (3000 r/min, 10 min), get supernatant liquor, 95% (v/v) washing with alcohol precipitation that adds 5 times of volumes, the dissolving of precipitation adding distil water, evenly rear centrifugal (3000 r/min of absolute ethyl alcohol and stirring that add again 5 times of volumes, 10 min), the precipitation lyophilize, make the dry raw polysaccharide of 3.103g, the waste liquid that produces in the recovery experiment process.
Embodiment 2: the blue root Crude polysaccharides of preparation Mohs.
With the Mohs orchid root that 100.0g cleans, dries, shreds, ultrasonic with 50 ℃ of 4000ml distilled water, ultrasonic frequency 45kHz, the ultrasonic power ratio is 70%.heating 2h, filter, filter residue repeats to extract 2 times, merge 3 filtrates and be evaporated to 100ml under 70 ℃, concentrated solution adds 95% (v/v) ethanol, make the ethanol volumn concentration reach 80%, fully after mixing, 4 ℃ of refrigerator cold-storage 24 h, then remove supernatant liquor, to precipitate centrifugal (3000 r/min, 15 min) after, get the dissolving of precipitation adding distil water, standing, centrifugal (3000 r/min, 10 min), get supernatant liquor, 95% (v/v) washing with alcohol precipitation that adds 8 times of volumes, the dissolving of precipitation adding distil water, evenly rear centrifugal (3000 r/min of absolute ethyl alcohol and stirring that add again 8 times of volumes, 15 min), the precipitation lyophilize, make the dry raw polysaccharide of 3.614g, the waste liquid that produces in the recovery experiment process.
Embodiment 3: the blue root Crude polysaccharides of preparation Mohs.
With the Mohs orchid root that 100.0g cleans, dries, shreds, ultrasonic with 60 ℃ of 1000ml distilled water, ultrasonic frequency 45kHz, the ultrasonic power ratio is 75%.heating 2h, filter, filter residue repeats to extract 2 times, merge 3 filtrates and be evaporated to 100ml under 80 ℃, concentrated solution adds 90% (v/v) ethanol, make the ethanol volumn concentration reach 80%, fully after mixing, 4 ℃ of refrigerator cold-storage 24 h, then remove supernatant liquor, to precipitate centrifugal (3000 r/min, 15 min) after, get the dissolving of precipitation adding distil water, standing, centrifugal (3000 r/min, 10 min), get supernatant liquor, 90% (v/v) washing with alcohol precipitation that adds 4 times of volumes, the dissolving of precipitation adding distil water, evenly rear centrifugal (3000 r/min of absolute ethyl alcohol and stirring that add again 4 times of volumes, 15 min), the precipitation lyophilize, make the dry raw polysaccharide of 2.919g, the waste liquid that produces in the recovery experiment process.
Embodiment 4: sucting wet experiment.
Add saturated (NH bottom moisture eliminator
4)
2SO
4The aqueous solution (RH=81%, RH are relative humidity) and saturated Na
2CO
3The aqueous solution (RH=43%) carries out fuchsin(e)test.Moisture eliminator is placed in 25 ℃ of thermostatic drying chambers, and l.0g precision takes 2 parts of the blue root Crude polysaccharides of dry Mohs and 1.0g glycerine, puts into respectively the weighing bottle that diameter is 40*25mm, uncap.Every 24h, weighing bottle is taken out and weigh, each sample horizontal survey 3 times is by the weight difference calculating rate of moisture absorption of sample before and after measuring.
Rate of moisture absorption (%)=100 (M
nOne M
0)/M
0
In formula, M
0For sample is placed front weight, M
nFor placing weight after n days.
Experimental result:
Mohs orchid root Crude polysaccharides is carried out hygroscopic property research, the results are shown in Figure 1, Fig. 2, as can be seen from the figure, in the environment of relative humidity 43% and 81%, in 144 h, the Crude polysaccharides rate of moisture absorption is greater than the glycerine rate of moisture absorption, illustrate, Mohs orchid root Crude polysaccharides contains extremely strong hydrophilic radical, and still has stronger hygroscopic property and be better than glycerine in the lower environment of relative humidity, rate of moisture absorption extends in time and increases, in 144 h, Sample moisture does not reach capacity, and humidity is larger, and the rate of moisture absorption of sample is larger.
Embodiment 3: the moisturizing experiment.
Adopt in vitro method to performance of keeping humidity and 5% glycerine (in makeup, glycerol content is generally 5%) of Mohs orchid root polysaccharide crude extract, compare.Mohs orchid root crude extract, glycerine are made into respectively 1%(w%), 5%(w%) aqueous solution, select the sheet glass of 7.5 cm*7.5 cm, paste one deck medical air-permeable adhesive tape in the above, draw 100 μ L with liquid-transfering gun, evenly be coated on breathable adhesive tape, put in the moisture eliminator of fixed temperature and humidity (RH=43%) and weigh respectively after certain hour, calculate percentage of water loss.In certain hour, percentage of water loss is less, and humidity-holding effect is better.Test repeats 3 times.
Percentage of water loss (%)=(M
1-M
t)/(M
1-M
0) * 100%
Wherein, M
1, M
tWeight after being respectively the starting weight of sample and placing for some time, M
0Be the sheet glass weight of placing.
Experimental result:
According to the difference of wetting Agent for Printing Inks performance of keeping humidity, different wetting Agent for Printing Inks molecules are different to the reactive force of water molecules, absorb moisture and keep the ability of moisture also different.Reactive force is large, and is strong to water molecules power, absorbs and keeps the amount of water also larger.Mohs orchid root Crude polysaccharides is carried out performance of keeping humidity research, the results are shown in Figure 3, as can be seen from the figure the percentage of water loss of 1% sample and 5% glycerine all increases with the prolongation of storage period, and in 16h, 1% Mohs orchid root Crude polysaccharides extract performance of keeping humidity is better than the performance of keeping humidity of 5% glycerine.
Sum up:
Mohs orchid root Crude polysaccharides is carried out performance of keeping humidity research, and 1% Mohs orchid root Crude polysaccharides solution performance of keeping humidity is better than wetting Agent for Printing Inks 5% glycerine commonly used, is Mohs orchid root Crude polysaccharides wettability power in 144h under 43% and 81% envrionment conditions at RH〉the glycerine wettability power.Mohs orchid root Crude polysaccharides shows good moisture absorption and performance of keeping humidity, thereby is a kind of natural moisture preserving agent that potentiality to be exploited is arranged very much.
Claims (8)
1. a crude polysaccharide extract of mokara root, is characterized in that, it is prepared by following steps:
(1) the blue root of Mohs is placed in distilled water, extracts 1～2h under 50～60 ℃, ultrasound condition, filter, filter residue repeats to merge 3 times filtrate with distilled water extraction 2 times;
(2) filtrate that step (1) is obtained is at 70～80 ℃ of lower concentrating under reduced pressure, adds in the concentrated solution ethanolic soln volumn concentration of ethanol to the mixed solution to reach 80%, fully after mixing, refrigerates 12～24h under 4 ℃;
(3) remove the supernatant liquor of the mixture after step (2) refrigerates, remaining precipitation is got precipitation after centrifugal, the adding distil water dissolving, standing, then get supernatant liquor after centrifugal, add ethanolic soln to stir, get precipitation after centrifugal, the adding distil water dissolving, then add absolute ethyl alcohol and stirring even, get precipitation after centrifugal, through lyophilize and get final product.
2. crude polysaccharide extract of mokara root according to claim 1, is characterized in that, in step (1), the blue root of described Mohs is the blue root of the Mohs after shredding.
3. crude polysaccharide extract of mokara root according to claim 1, is characterized in that, in step (1), for the blue root of every g Mohs, the volumetric usage of distilled water is 10～40mL.
4. crude polysaccharide extract of mokara root according to claim 1, is characterized in that, in step (2) or (3), described volumes of aqueous ethanol percentage concentration is 90～95%.
5. crude polysaccharide extract of mokara root according to claim 1, is characterized in that, in step (3), the volume that adds ethanolic soln is 4～8 times of supernatant liquor volume to be added.
6. crude polysaccharide extract of mokara root according to claim 1, is characterized in that, in step (3), the volume that adds of dehydrated alcohol is 4～8 times of system volume to be added.
7. crude polysaccharide extract of mokara root according to claim 1, is characterized in that, in step (3), centrifugal condition is 3000r/min, 10～15min.
8. the application of crude polysaccharide extract of mokara root claimed in claim 1 in moisturizing, moisture absorption field.
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| CN113768822B (en) * | 2021-10-25 | 2024-01-30 | 广州煜明生物科技有限公司 | Application of hedysarum polybasic polysaccharide in preparation of cosmetics with moisture absorption, moisturizing or relieving effects |
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| CN101249060A (en) * | 2008-03-28 | 2008-08-27 | 钱国英 | Multiple-effect washing cream and method of preparing the same |
| CN102274247A (en) * | 2011-07-19 | 2011-12-14 | 南通大学 | Laminaria polysaccharide japonica capsules and preparation method and application thereof |
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| CN101249060A (en) * | 2008-03-28 | 2008-08-27 | 钱国英 | Multiple-effect washing cream and method of preparing the same |
| CN102274247A (en) * | 2011-07-19 | 2011-12-14 | 南通大学 | Laminaria polysaccharide japonica capsules and preparation method and application thereof |
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