CN102532342A - Crude polysaccharide extract of mokara root and application - Google Patents
Crude polysaccharide extract of mokara root and application Download PDFInfo
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- CN102532342A CN102532342A CN2012100858333A CN201210085833A CN102532342A CN 102532342 A CN102532342 A CN 102532342A CN 2012100858333 A CN2012100858333 A CN 2012100858333A CN 201210085833 A CN201210085833 A CN 201210085833A CN 102532342 A CN102532342 A CN 102532342A
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 53
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 53
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 53
- 239000000284 extract Substances 0.000 title claims abstract description 27
- 241001184535 x Mokara Species 0.000 title abstract description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 42
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 31
- 239000006228 supernatant Substances 0.000 claims abstract description 15
- 238000002156 mixing Methods 0.000 claims abstract description 7
- 238000002604 ultrasonography Methods 0.000 claims abstract description 6
- 239000012153 distilled water Substances 0.000 claims abstract description 5
- 239000000203 mixture Substances 0.000 claims abstract description 4
- 239000011259 mixed solution Substances 0.000 claims abstract description 3
- 230000008021 deposition Effects 0.000 claims description 17
- 238000010521 absorption reaction Methods 0.000 claims description 14
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- 238000003756 stirring Methods 0.000 claims description 7
- 238000005057 refrigeration Methods 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 2
- 230000001476 alcoholic effect Effects 0.000 claims description 2
- 238000003809 water extraction Methods 0.000 claims description 2
- 239000002244 precipitate Substances 0.000 abstract description 8
- 241000196324 Embryophyta Species 0.000 abstract description 6
- 239000000243 solution Substances 0.000 abstract description 4
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 238000005119 centrifugation Methods 0.000 abstract 4
- 238000004090 dissolution Methods 0.000 abstract 2
- 239000000706 filtrate Substances 0.000 abstract 2
- 238000001914 filtration Methods 0.000 abstract 2
- 238000004108 freeze drying Methods 0.000 abstract 1
- 230000001376 precipitating effect Effects 0.000 abstract 1
- 241000233855 Orchidaceae Species 0.000 description 27
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 23
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- 239000000976 ink Substances 0.000 description 11
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- 238000011161 development Methods 0.000 description 4
- 239000003755 preservative agent Substances 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
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- 238000005516 engineering process Methods 0.000 description 3
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- 235000016709 nutrition Nutrition 0.000 description 3
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
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- 230000035922 thirst Effects 0.000 description 2
- MEIRRNXMZYDVDW-MQQKCMAXSA-N (2E,4E)-2,4-hexadien-1-ol Chemical compound C\C=C\C=C\CO MEIRRNXMZYDVDW-MQQKCMAXSA-N 0.000 description 1
- AXDJCCTWPBKUKL-UHFFFAOYSA-N 4-[(4-aminophenyl)-(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]aniline;hydron;chloride Chemical compound Cl.C1=CC(=N)C(C)=CC1=C(C=1C=CC(N)=CC=1)C1=CC=C(N)C=C1 AXDJCCTWPBKUKL-UHFFFAOYSA-N 0.000 description 1
- 241000239290 Araneae Species 0.000 description 1
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- 230000003020 moisturizing effect Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
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- 150000002989 phenols Chemical class 0.000 description 1
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- 239000011734 sodium Substances 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
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- Polysaccharides And Polysaccharide Derivatives (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a crude polysaccharide extract of a mokara root, which is obtained by the following steps: placing the mokara root in distilled water, extracting for 1-2h under the condition of ultrasound at the temperature of 50-60 DEG C, filtering, filtering residue, extracting for twice, and blending the filtrate for three times; carrying out pressure-reducing concentration to the filtrate, adding ethanol solution to the concentrated solution until ethanol in the mixed solution accounts for 80% by volume, fully and evenly mixing, and refrigerating for 12-24h at the temperature of 4 DEG C; removing supernatant of the refrigerated mixture, precipitating, taking a precipitate after centrifugation, adding distilled water to perform dissolution, standing, taking the supernatant after centrifugation, adding ethanol solution, taking the precipitate after centrifugation, adding distilled water to perform dissolution, adding absolute ethanol, taking the supernatant after centrifugation, and obtaining the crude polysaccharide extract after freeze drying. The crude polysaccharide extract of the mokara root is all natural plant polysaccharide, and not only has the excellent moisture-preserving and moisture-absorbing performance, but also meets the requirements of users in the aspects of safety and environmental protection.
Description
Technical field
The invention belongs to technical field of plant extraction, be specifically related to blue root Crude polysaccharides extract of a kind of Mohs and application.
Background technology
The research of preserving moisture is the focus of cosmetic conduct and learning, dermatology.The effect of wetting Agent for Printing Inks in makeup is the water-content of keeping or increase keratoderma, improves clinical symptom such as the xerosis cutis that causes because of the stratum corneum lack of water and consequent is itched, furfur.Wetting Agent for Printing Inks can be divided into natural moisture preserving agent and synthetic wetting Agent for Printing Inks by its source, can be divided into general wetting Agent for Printing Inks, softener, deep moisturizing agent again by its mechanism of action.The wetting Agent for Printing Inks that uses in the makeup at present is mainly polyvalent alcohol, sorbyl alcohol, lactic acid and sodium salt thereof etc.From crude substance, extract have nutrition and the natural moisture preserving agent of the dual performance of preserving moisture meets following wetting Agent for Printing Inks Development Trend.
Polysaccharide is to be present in the polymkeric substance that natural aldose or ketose link together through glycosidic link.Wide at the occurring in nature distributed pole, be prevalent in plant, animal, mikrobe and the marine alga, mainly contain vegetable polysaccharides, animal polysaccharide, microbial polysaccharide, Sargassum polysaccharides etc.From crude substance, extract in recent years and have nutrition and the natural moisture preserving agent of the dual-use function of preserving moisture is the focus of studying, natural polysaccharide is one type of rare material which can retain moisture.Polysaccharide is applied to makeup as wetting Agent for Printing Inks. satisfied the requirement of natural, nutrition that people propose makeup, nonirritant etc.
The Mohs orchid (
Mokara) be the orchid family herbage, be 1969 by the Singaporean with blue, the bird tongue blue, blue three genus of spider hybridize out novel orchid all ages.This is one type of happiness light, and growing way is strong, and output is high, and spray is long, and pattern is gorgeous, is well suited for doing the orchid of cut-flower, and has certain pharmaceutical use.Broken away from that the Mohs of soil is blue main to be leaned on root from air, to absorb moisture and survive, the Mohs orchid has luxuriant aerial root, and fast growth can reach several meters.For increasing yield and quality, the flower-grower need regularly prune these aged, unnecessary aerial root, thereby produces a large amount of discarded roots.
Find that through inspection information orchid is not only precious ornamental plant, but also be important medicinal plant source.According to the Compendium of Material Medica record, " fragrant thoroughwort, smell is hot, flat, sweet, nontoxic.Its gas delicate fragrance, promote the production of body fluid to quench thirst, profit muscle is controlled the ehat in the gallbladder channel of quenching one's thirst ".According to reported in literature, mainly contain compounds such as polysaccharide, phenols, terpene, flavones in the orchid.The Mohs orchid is the novel cymbidium variety of hybridization, and reported in literature is not also seen in the research of its composition and effect, and carrying out the extraction of Mohs orchid root polysaccharide and the activity research of preserving moisture will be a very significant job.Using ultrasound ripple method is extracted the Crude polysaccharides of Mohs orchid root; And the performance of keeping humidity of using wetting Agent for Printing Inks glycerine in the Crude polysaccharides that extracts and the makeup always compared research; Explore its performance of keeping humidity; Thereby being turned waste into wealth, the Mohs orchid root is applied to the production of makeup, for comprehensive development and utilization torrid zone Mohs orchid provides scientific basis, for the development orchid industry provides new thinking.
Summary of the invention
Technical problem to be solved by this invention provides a kind of in the blue root Crude polysaccharides of a kind of Mohs extract is provided.
The technical problem that the present invention also will solve provides uses tropical Mohs orchid root Crude polysaccharides, can moisture absorption and the effect of preserving moisture.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is following:
The blue root Crude polysaccharides of a kind of Mohs extract, it is prepared by following steps:
(1) the blue root of Mohs is placed zero(ppm) water, 50 ~ 60 ℃, ultrasound condition extract 1 ~ 2h down, filter, and filter residue repeats to merge filtrating 3 times with distilled water extraction 2 times;
(2) filtrating that step (1) is obtained is at 70 ~ 80 ℃ of following concentrating under reduced pressure, adds in liquid concentrator that the alcoholic acid volumn concentration reaches 80% in ethanolic soln to the mixed solution, fully behind the mixing, and 4 ℃ of refrigeration 12 ~ 24 h down;
(3) remove the supernatant of the mixture after step (2) refrigeration, remaining deposition is got deposition after centrifugal, the adding distil water dissolving; Leave standstill, after centrifugal, get supernatant again, add ethanolic soln and stir; Get deposition after centrifugal, adding distil water dissolving, it is even to add absolute ethyl alcohol and stirring again; Get deposition after centrifugal, promptly get through lyophilize.
In the step (1), the blue root of described Mohs is the blue root of the Mohs after shredding.
In the step (1), for the blue root of every g Mohs, the volumetric usage of zero(ppm) water is 10 ~ 40mL.
In the step (1), ultrasound condition is ultrasonic frequency 45kHz, and the ultrasonic power ratio is 70 ~ 80%.
In step (2) or (3), described volumes of aqueous ethanol percentage concentration is 90 ~ 95%.
In the step (3), the volume that adds ethanolic soln is 4 ~ 8 times of supernatant volume to be added.
In the step (3), the adding volume of absolute ethyl alcohol is 4 ~ 8 times of system volume to be added.
In the step (3), centrifugal condition is 3000 r/min, 10 ~ 15 min.
The blue root Crude polysaccharides of above-mentioned Mohs extract preserve moisture, the moisture absorption Application for Field.
The application of the blue root Crude polysaccharides extract of above-mentioned Mohs in moisture-keeping cosmetics or medicine.
The application of the blue root Crude polysaccharides extract of above-mentioned Mohs in moisture absorption makeup or medicine.
Beneficial effect: the blue root Crude polysaccharides of Mohs of the present invention extract is the pure natural plants polysaccharide, its not only have good preserve moisture, hygroscopic property, and satisfied the demand of people aspect security, environmental protection.Technology of the present invention is that the development and use of the blue root Crude polysaccharides of Mohs provide reference.
Description of drawings
Fig. 1 RH is 43% o'clock different sample rate of moisture absorption and time chart.
Fig. 2 RH is 81% o'clock different sample rate of moisture absorption and time chart.
Fig. 3 is sample percentage of water loss and time chart.
Embodiment
According to following embodiment, can understand the present invention better.Yet, those skilled in the art will readily understand that the described content of embodiment only is used to explain the present invention, and the present invention that should also can not limit in claims to be described in detail.
Embodiment 1: the blue root Crude polysaccharides of preparation Mohs.
With the Mohs orchid root that 100.0g cleans, dries, shreds, ultrasonic with 60 ℃ of 2000ml zero(ppm) water, ultrasonic frequency 45kHz, the ultrasonic power ratio is 80%; Heating 1h filters, and filter residue repeats to extract 2 times, merges 3 filtratings and under 80 ℃, is evaporated to 100ml; Liquid concentrator adds 95% (v/v) ethanol of 5 times of volumes, makes the ethanol volumn concentration reach 80%, behind the abundant mixing, and 4 ℃ of refrigerator cold-storage 12 h; Remove supernatant then, will precipitate centrifugal (3000 r/min, 10 min) after, get deposition adding distil water dissolving; Leave standstill, centrifugal (3000 r/min, 10 min) get supernatant; Add 95% (v/v) washing with alcohol deposition of 5 times of volumes, the deposition adding distil water dissolves, and adds the even back of absolute ethyl alcohol and stirring centrifugal (3000 r/min, 10 min) of 5 times of volumes again; Precipitate lyophilize, make the dry raw polysaccharide of 3.103g, reclaim the waste liquid that produces in the experimentation.
Embodiment 2: the blue root Crude polysaccharides of preparation Mohs.
With the Mohs orchid root that 100.0g cleans, dries, shreds, ultrasonic with 50 ℃ of 4000ml zero(ppm) water, ultrasonic frequency 45kHz, the ultrasonic power ratio is 70%.Heating 2h filters, and filter residue repeats to extract 2 times, merges 3 filtratings and under 70 ℃, is evaporated to 100ml; Liquid concentrator adds 95% (v/v) ethanol, makes the ethanol volumn concentration reach 80%, behind the abundant mixing, and 4 ℃ of refrigerator cold-storage 24 h; Remove supernatant then, will precipitate centrifugal (3000 r/min, 15 min) after, get deposition adding distil water dissolving; Leave standstill, centrifugal (3000 r/min, 10 min) get supernatant; Add 95% (v/v) washing with alcohol deposition of 8 times of volumes, the deposition adding distil water dissolves, and adds the even back of absolute ethyl alcohol and stirring centrifugal (3000 r/min, 15 min) of 8 times of volumes again; Precipitate lyophilize, make the dry raw polysaccharide of 3.614g, reclaim the waste liquid that produces in the experimentation.
Embodiment 3: the blue root Crude polysaccharides of preparation Mohs.
With the Mohs orchid root that 100.0g cleans, dries, shreds, ultrasonic with 60 ℃ of 1000ml zero(ppm) water, ultrasonic frequency 45kHz, the ultrasonic power ratio is 75%.Heating 2h filters, and filter residue repeats to extract 2 times, merges 3 filtratings and under 80 ℃, is evaporated to 100ml; Liquid concentrator adds 90% (v/v) ethanol, makes the ethanol volumn concentration reach 80%, behind the abundant mixing, and 4 ℃ of refrigerator cold-storage 24 h; Remove supernatant then, will precipitate centrifugal (3000 r/min, 15 min) after, get deposition adding distil water dissolving; Leave standstill, centrifugal (3000 r/min, 10 min) get supernatant; Add 90% (v/v) washing with alcohol deposition of 4 times of volumes, the deposition adding distil water dissolves, and adds the even back of absolute ethyl alcohol and stirring centrifugal (3000 r/min, 15 min) of 4 times of volumes again; Precipitate lyophilize, make the dry raw polysaccharide of 2.919g, reclaim the waste liquid that produces in the experimentation.
Embodiment 4: sucting wet experiment.
Add saturated (NH in the moisture eliminator bottom
4)
2SO
4The aqueous solution (RH=81%, RH are relative humidity) and saturated Na
2CO
3The aqueous solution (RH=43%) carries out fuchsin(e)test.Moisture eliminator places 25 ℃ of thermostatic drying chambers, and precision takes by weighing 2 parts of the blue root Crude polysaccharides of l.0g dry Mohs and 1.0g glycerine, puts into the weighing bottle that diameter is 40*25mm respectively, uncap.Every weighing bottle the taking-up at a distance from 24h weighed, and each sample horizontal survey 3 times is calculated rate of moisture absorption by the weight difference of measuring the front and back sample.
Rate of moisture absorption (%)=100 (M
nOne M
0)/M
0
In the formula, M
0For sample is placed preceding weight, M
nFor placing weight after n days.
Experimental result:
Mohs orchid root Crude polysaccharides is carried out hygroscopic property research, and the result sees Fig. 1, Fig. 2, as can be seen from the figure; In the environment of relative humidity 43% and 81%, the Crude polysaccharides rate of moisture absorption is greater than glycerine rate of moisture absorption, explanation in 144 h; Mohs orchid root Crude polysaccharides contains extremely strong hydrophilic radical, and in the lower environment of relative humidity, still has stronger hygroscopic property and be superior to glycerine, and rate of moisture absorption prolongs in time and increases; Sample humidity does not reach capacity in 144 h, and humidity is bigger, and the rate of moisture absorption of sample is bigger.
Embodiment 3: the experiment of preserving moisture.
Adopt performance of keeping humidity and 5% glycerine (in makeup glycerol content be generally 5%) of in vitro method, compare Mohs orchid root polysaccharide crude extract.Mohs orchid root crude extract, glycerine are made into 1% (w%), 5% (w%) aqueous solution respectively; Select the sheet glass of 7.5 cm*7.5 cm for use; Paste one deck medical air-permeable adhesive tape in the above, draw 100 μ L, evenly be coated on the breathable adhesive tape with liquid-transfering gun; Put in the moisture eliminator of fixed temperature and humidity (RH=43%) and weigh respectively behind the certain hour, calculate percentage of water loss.In the certain hour, percentage of water loss is more little, and humidity-holding effect is good more.Test repetition 3 times.
Percentage of water loss (%)=(M
1-M
t)/(M
1-M
0) * 100%
Wherein, M
1, M
tWeight after being respectively the starting weight of sample and placing for some time, M
0Be the sheet glass weight of placing.
Experimental result:
According to the difference of wetting Agent for Printing Inks performance of keeping humidity, different wetting Agent for Printing Inks molecules are different to the reactive force of water molecules, and it is also different with the ability that keeps moisture to absorb moisture.Reactive force is big, and is strong to water molecules power, and the amount of absorption and maintenance water is also bigger.Mohs orchid root Crude polysaccharides is carried out performance of keeping humidity research; The result sees Fig. 3; As can be seen from the figure the percentage of water loss of 1% sample and 5% glycerine all increases with the prolongation of storage period, and 1% Mohs orchid root Crude polysaccharides extract performance of keeping humidity is superior to the performance of keeping humidity of 5% glycerine in the 16h.
Sum up:
Mohs orchid root Crude polysaccharides is carried out performance of keeping humidity research, and 1% Mohs orchid root Crude polysaccharides solution performance of keeping humidity is superior to wetting Agent for Printing Inks 5% glycerine commonly used, is Mohs orchid root Crude polysaccharides wettability power in the 144h under 43% and 81% the envrionment conditions at RH>the glycerine wettability power.Mohs orchid root Crude polysaccharides shows good moisture absorption and performance of keeping humidity, thereby is a kind of natural moisture preserving agent that potentiality to be exploited is arranged very much.
Claims (9)
1. the blue root Crude polysaccharides of Mohs extract is characterized in that it is prepared by following steps:
(1) the blue root of Mohs is placed zero(ppm) water, 50 ~ 60 ℃, ultrasound condition extract 1 ~ 2h down, filter, and filter residue repeats to merge filtrating 3 times with distilled water extraction 2 times;
(2) filtrating that step (1) is obtained is at 70 ~ 80 ℃ of following concentrating under reduced pressure, adds in liquid concentrator that the alcoholic acid volumn concentration reaches 80% in ethanolic soln to the mixed solution, fully behind the mixing, and 4 ℃ of refrigeration 12 ~ 24 h down;
(3) remove the supernatant of the mixture after step (2) refrigeration, remaining deposition is got deposition after centrifugal, the adding distil water dissolving; Leave standstill, after centrifugal, get supernatant again, add ethanolic soln and stir; Get deposition after centrifugal, adding distil water dissolving, it is even to add absolute ethyl alcohol and stirring again; Get deposition after centrifugal, promptly get through lyophilize.
2. the blue root Crude polysaccharides of Mohs according to claim 1 extract is characterized in that, in the step (1), the blue root of described Mohs is the blue root of the Mohs after shredding.
3. the blue root Crude polysaccharides of Mohs according to claim 1 extract is characterized in that, in the step (1), for the blue root of every g Mohs, the volumetric usage of zero(ppm) water is 10 ~ 40mL.
4. the blue root Crude polysaccharides of Mohs according to claim 1 extract is characterized in that in the step (1), ultrasound condition is ultrasonic frequency 45kHz, and the ultrasonic power ratio is 70 ~ 80%.
5. the blue root Crude polysaccharides of Mohs according to claim 1 extract is characterized in that in step (2) or (3), described volumes of aqueous ethanol percentage concentration is 90 ~ 95%.
6. the blue root Crude polysaccharides of Mohs according to claim 1 extract is characterized in that, in the step (3), the volume that adds ethanolic soln is 4 ~ 8 times of supernatant volume to be added.
7. the blue root Crude polysaccharides of Mohs according to claim 1 extract is characterized in that in the step (3), the adding volume of absolute ethyl alcohol is 4 ~ 8 times of system volume to be added.
8. the blue root Crude polysaccharides of Mohs according to claim 1 extract is characterized in that in the step (3), centrifugal condition is 3000 r/min, 10 ~ 15 min.
The blue root Crude polysaccharides of the described Mohs of claim 1 extract preserve moisture, the moisture absorption Application for Field.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102793648A (en) * | 2012-09-05 | 2012-11-28 | 三亚柏盈热带兰花产业有限公司 | Morse cymbidium root extract and applications thereof |
| CN113768822A (en) * | 2021-10-25 | 2021-12-10 | 广州煜明生物科技有限公司 | Application of radix hedysari polysaccharide in preparation of cosmetics with moisture absorption, moisture retention or relieving effects |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101249060A (en) * | 2008-03-28 | 2008-08-27 | 钱国英 | Multiple-effect washing cream and method of preparing the same |
| CN102274247A (en) * | 2011-07-19 | 2011-12-14 | 南通大学 | Laminaria polysaccharide japonica capsules and preparation method and application thereof |
-
2012
- 2012-03-28 CN CN2012100858333A patent/CN102532342B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101249060A (en) * | 2008-03-28 | 2008-08-27 | 钱国英 | Multiple-effect washing cream and method of preparing the same |
| CN102274247A (en) * | 2011-07-19 | 2011-12-14 | 南通大学 | Laminaria polysaccharide japonica capsules and preparation method and application thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102793648A (en) * | 2012-09-05 | 2012-11-28 | 三亚柏盈热带兰花产业有限公司 | Morse cymbidium root extract and applications thereof |
| CN113768822A (en) * | 2021-10-25 | 2021-12-10 | 广州煜明生物科技有限公司 | Application of radix hedysari polysaccharide in preparation of cosmetics with moisture absorption, moisture retention or relieving effects |
| CN113768822B (en) * | 2021-10-25 | 2024-01-30 | 广州煜明生物科技有限公司 | Application of hedysarum polybasic polysaccharide in preparation of cosmetics with moisture absorption, moisturizing or relieving effects |
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