CN102793648B - Morse cymbidium root extract and applications thereof - Google Patents
Morse cymbidium root extract and applications thereof Download PDFInfo
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- CN102793648B CN102793648B CN2012103235122A CN201210323512A CN102793648B CN 102793648 B CN102793648 B CN 102793648B CN 2012103235122 A CN2012103235122 A CN 2012103235122A CN 201210323512 A CN201210323512 A CN 201210323512A CN 102793648 B CN102793648 B CN 102793648B
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Abstract
The invention relates to a morse cymbidium root extract and applications of the morse cymbidium root extract in sunscreen products. The morse cymbidium root extract is prepared through the following steps of: crushing the morse cymbidium root extract, arranging the crushed morse cymbidium root extract into acetone to conduct ultrasonic extraction, filtering, decompressing and concentrating a filtrate to be a concrete, and freezing and drying the concrete. According to the morse cymbidium root extract, the process is simple, the morse cymbidium root is extracted effectively by adopting an organic solvent and ultrasonic waves, the obtained extract has a good ultraviolet absorbing property, meets the requirements on safety and environmental protection caused by people, can be effectively used for preparing sunscreen products, provides the reference for the development and utilization of the morse cymbidium root extract, and also provides a new approach for the cymbidium industry.
Description
Technical field
The invention belongs to technical field of plant extraction, be specifically related to a kind of Mohs Cymbidium ensifolium (L.) Sw. root extract and the application in sunscreen product thereof.
Background technology
Sun-proof research is the focus of cosmetic conduct and learning, dermatology.The effect of sunscreen in cosmetics is that pre-anti-ultraviolet is to the hurtful important measures of human body.The ultraviolet screener material of application is divided into two classes at present: physical barrier agent and chemical absorbent.Chemical absorbent is called again UV absorbent, is divided into two kinds of chemosynthesis and natural extract.The UV absorbent of chemosynthesis such as cinnamic acid, cinnamate, salicylate, benzophenone derivates etc.; The UV absorbent of natural extract refers to from the natural ultraviolet absorber compounds that extracts plant, as: flavone compound, anthraquinone analog compound, plant polyphenol compounds and derivant thereof etc.Because there is certain toxic and side effects in the UV absorbent of chemosynthesis in application, so the research of natural, nontoxic UV absorbent application more and more gets more and more people's extensive concerning.
Mohs blue (mokara) be the orchid family herbaceous plant, is the novel Cymbidium ensifolium (L.) Sw. that was hybridized out with all ages blue, bird tongue orchid, three genus of Hymenocallis americana by the Singaporean in 1969.This is a class happiness light, and growing way is strong, and output is high, and spray is long, and pattern is gorgeous, is well suited for doing the orchid of cut-flower, and has certain medical value.The blue main root that leans on of Mohs that has broken away from soil is survived from air, absorbing moisture, and the Mohs orchid has luxuriant aerial root, and fast growth can reach several meters.For increasing yield and quality, the flower-grower need to be aging to these, unnecessary aerial root is regularly pruned, thereby produced a large amount of discarded roots.
Through inspection information, find, Cymbidium ensifolium (L.) Sw. is not only precious ornamental plant, but also is important Source of Drug Plants.According to the Compendium of Material Medica record, " Herba Chlorophyti, abnormal smells from the patient is hot, flat, sweet, nontoxic.Its gas delicate fragrance, promoting the production of body fluid to quench thirst, profit muscle, control the endogenous heat syndrome involving the gallbladder of quenching one's thirst ".According to reported in literature, in Cymbidium ensifolium (L.) Sw., mainly contain the compounds such as polysaccharide, phenols, terpenoid, flavone.The Mohs orchid is the novel cymbidium variety of hybridization, and it is formed and the research of effect yet there are no reported in literature, and carrying out Mohs Cymbidium ensifolium (L.) Sw. root extract and sunscreen actives research will be a very significant job.
Summary of the invention
The purpose of this invention is to provide that a kind of preparation method is simple, the Mohs Cymbidium ensifolium (L.) Sw. root extract of pure natural, adopt organic solvent and ultrasound wave to extract, it not only has the ultraviolet performance of good absorption, and met the demand of people aspect safety, environmental protection, for the exploitation of Mohs Cymbidium ensifolium (L.) Sw. root provide reference.
The technical solution adopted in the present invention:
A kind of Mohs Cymbidium ensifolium (L.) Sw. root extract, be to get the pulverizing of Mohs Cymbidium ensifolium (L.) Sw. root to be placed in acetone, at 30 ℃～60 ℃ of temperature, ultrasonic power, is that 75～220W, frequency are under the condition of 25～45 Hz, supersound extraction 20～80min; Filter, filtering residue repeats to extract 2 times, merging filtrate; Filtrate is evaporated to extractum under 40～50 ℃, through lyophilization, obtains Mohs Cymbidium ensifolium (L.) Sw. root extract.
In above-mentioned preparation method, acetone is 40～100mL/g with the volume mass ratio of Mohs Cymbidium ensifolium (L.) Sw. root extract.
The present invention also comprises the application of above-mentioned Mohs Cymbidium ensifolium (L.) Sw. root extract in preparing sunscreen product.
By the above-mentioned Mohs Cymbidium ensifolium (L.) Sw. root extract for preparing and existing sunscreen product substrate (as: tristerin 3%, stearic acid 7%, hexadecanol 5%, vaseline 1%, sodium lauryl sulphate 0.5%, Borax 1.5%, Buddhist nun's platinum methyl ester 0.1%, glycerol 3% commonly used, surplus is distilled water) carry out compatibility, prepare the sunscreen cream that contains Mohs Cymbidium ensifolium (L.) Sw. root extract.Sunscreen cream to preparation carries out ultraviolet spectra research, has and absorbs preferably ultraviolet ability, proves that the sunscreen cream take Mohs Cymbidium ensifolium (L.) Sw. root extract as sunscreen has obvious sun-proof result.
Described sunscreen product is sunscreen cream, sunscreen cream substrate, Mohs Cymbidium ensifolium (L.) Sw. root extract solution, consists of, and wherein, the concentration of Mohs Cymbidium ensifolium (L.) Sw. root extract solution is 400 μ g/ml solution, and the mass ratio of sunscreen cream substrate, Mohs Cymbidium ensifolium (L.) Sw. root extract solution is: 75:25～20.Sunscreen cream substrate can be existing any sunscreen cream substrate.
In the present invention, can in above-mentioned sunscreen cream, add in mass ratio as required the water below 5 umbers.
Technique of the present invention is simple, adopt organic solvent and ultrasound wave effectively to extract the Mohs Cymbidium ensifolium (L.) Sw. root, extract obtained is the plant extract of pure natural, not only has the ultraviolet performance of good absorption, and met the demand of people aspect safety, environmental protection, for the exploitation of Mohs Cymbidium ensifolium (L.) Sw. root provide reference, for the development orchid industry provide new by way of.
The specific embodiment
Below in conjunction with embodiment, the specific embodiment of the present invention is described in further detail.Following examples are used for the present invention is described, but are not used for limiting the scope of the invention.The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, or the condition of advising according to manufacturer.
Embodiment mono-
By the Mohs Cymbidium ensifolium (L.) Sw. root that 10.0g cleans, dries, shreds, with 40 ℃ of supersound extraction 40min of 400ml acetone, power is 80W, and frequency is 25 Hz.Filter, filtering residue repeats to extract 2 times, merges 3 filtrates and be evaporated to extractum under 50 ℃, and the concentrated solution lyophilization, make the dry extract of 0.703g, i.e. Mohs Cymbidium ensifolium (L.) Sw. root extract, the waste liquid that produces in the recovery experiment process.
Embodiment bis-
By the Mohs Cymbidium ensifolium (L.) Sw. root that 10.0g cleans, dries, shreds, with 40 ℃ of supersound extraction 60min of 600ml acetone, power is 200W, and frequency is 25 Hz.Filter, filtering residue repeats to extract 2 times, merges 3 filtrates and be evaporated to extractum under 40 ℃, and the concentrated solution lyophilization, make the dry extract of 0.826g, i.e. Mohs Cymbidium ensifolium (L.) Sw. root extract, the waste liquid that produces in the recovery experiment process.
Embodiment tri-
By the Mohs Cymbidium ensifolium (L.) Sw. root that 10.0g cleans, dries, shreds, with 50 ℃ of supersound extraction 60min of 800ml acetone, power is 220W, and frequency is 45 Hz.Filter, filtering residue repeats to extract 2 times, merges 3 filtrates and be evaporated to extractum under 50 ℃, and the concentrated solution lyophilization, make the dry extract of 1.206g, i.e. Mohs Cymbidium ensifolium (L.) Sw. root extract, the waste liquid that produces in the recovery experiment process.
Embodiment tetra-
By the Mohs Cymbidium ensifolium (L.) Sw. root that 10.0g cleans, dries, shreds, with 45 ℃ of supersound extraction 70min of 1000ml acetone, power is 180W, and frequency is 40 Hz.Filter, filtering residue repeats to extract 2 times, merges 3 filtrates and be evaporated to extractum under 45 ℃, and the concentrated solution lyophilization, make 1.137 dry extract, i.e. Mohs Cymbidium ensifolium (L.) Sw. root extract, the waste liquid that produces in the recovery experiment process.
Embodiment five
By the Mohs Cymbidium ensifolium (L.) Sw. root that 10.0g cleans, dries, shreds, with 30 ℃ of supersound extraction 30min of 800ml acetone, power is 120W, and frequency is 40 Hz.Filter, filtering residue repeats to extract 2 times, merges 3 filtrates and be evaporated to extractum under 50 ℃, and the concentrated solution lyophilization, make the dry extract of 0.796g, i.e. Mohs Cymbidium ensifolium (L.) Sw. root extract, the waste liquid that produces in the recovery experiment process.
Embodiment six
By the Mohs Cymbidium ensifolium (L.) Sw. root that 10.0g cleans, dries, shreds, with 50 ℃ of supersound extraction 60min of 800ml acetone, power is 220W, and frequency is 35 Hz.Filter, filtering residue repeats to extract 2 times, merges 3 filtrates and be evaporated to extractum under 40 ℃, and the concentrated solution lyophilization, make the dry extract of 1.021g, i.e. Mohs Cymbidium ensifolium (L.) Sw. root extract, the waste liquid that produces in the recovery experiment process.
Embodiment seven
By the Mohs Cymbidium ensifolium (L.) Sw. root that 10.0g cleans, dries, shreds, with 60 ℃ of supersound extraction 40min of 800ml acetone, power is 160W, and frequency is 45 Hz.Filter, filtering residue repeats to extract 2 times, merges 3 filtrates and be evaporated to extractum under 50 ℃, and the concentrated solution lyophilization, make the dry extract of 0.945g, i.e. Mohs Cymbidium ensifolium (L.) Sw. root extract, the waste liquid that produces in the recovery experiment process.
Embodiment eight
By the Mohs Cymbidium ensifolium (L.) Sw. root that 10.0g cleans, dries, shreds, with 40 ℃ of supersound extraction 20min of 800ml acetone, power is 180W, and frequency is 45 Hz.Filter, filtering residue repeats to extract 2 times, merges 3 filtrates and be evaporated to extractum under 45 ℃, and the concentrated solution lyophilization, make the dry extract of 0.725g, i.e. Mohs Cymbidium ensifolium (L.) Sw. root extract, the waste liquid that produces in the recovery experiment process.
Embodiment nine
By the Mohs Cymbidium ensifolium (L.) Sw. root that 10.0g cleans, dries, shreds, with 50 ℃ of supersound extraction 40min of 800ml acetone, power is 200W, and frequency is 45 Hz.Filter, filtering residue repeats to extract 2 times, merges 3 filtrates and be evaporated to extractum under 40 ℃, and the concentrated solution lyophilization, make the dry extract of 0.909g, i.e. Mohs Cymbidium ensifolium (L.) Sw. root extract, the waste liquid that produces in the recovery experiment process.
Embodiment ten
By the Mohs Cymbidium ensifolium (L.) Sw. root that 10.0g cleans, dries, shreds, with 60 ℃ of supersound extraction 80min of 800ml acetone, power is 220W, and frequency is 25 Hz.Filter, filtering residue repeats to extract 2 times, merges 3 filtrates and be evaporated to extractum under 50 ℃, and the concentrated solution lyophilization, make the dry extract of 1.015g, i.e. Mohs Cymbidium ensifolium (L.) Sw. root extract, the waste liquid that produces in the recovery experiment process.
Test and application:
One, absorb the ultraviolet experiment
Accurately weighing 0.01g Mohs Cymbidium ensifolium (L.) Sw. root extract is dissolved in the 25ml dehydrated alcohol, and being made into concentration is 400 μ g/ml Mohs Cymbidium ensifolium (L.) Sw. root extract solutions.Therefrom accurately measure 1ml solution in 10ml volumetric flask ethanol solution standardize solution, obtain 40 μ g/ml Mohs Cymbidium ensifolium (L.) Sw. root extract solutions.In like manner prepare 40 μ g/ml rutins and sunscreen (4-methyl benzal subunit camphor ball) solution, carry out respectively ultraviolet spectroscopy, record absorbance, the results are shown in Table 1.
Table 1 Mohs Cymbidium ensifolium (L.) Sw. root extract and rutin and sunscreen absorb the comparison of ultraviolet activity
| Title | UVA | UVB | UVC |
| Mohs Cymbidium ensifolium (L.) Sw. root (40 μ g/ml) | 0.098 | 0.227 | 1.109 |
| Mohs Cymbidium ensifolium (L.) Sw. root (400 μ g/ml) | 2.177 | 4.247 | 6.728 |
| Sunscreen (40 μ g/ml) | 0.511 | 3.349 | 3.485 |
| Rutin (40 μ g/ml) | 1.014 | 0.663 | 1.908 |
By table 1, can be drawn, when the concentration of Mohs Cymbidium ensifolium (L.) Sw. root extract was 40 μ g/ml, the mean light absorbency value in UVA, UBV, UVC interval was all low than corresponding sunscreen and rutin; When the concentration of extracting solution reached 400 μ g/ml, the mean light absorbency value in UVA, UBV, UVC interval was all high than the sunscreen of 40 μ g/ml and rutin.Therefore, can think, 400 μ g/ml Mohs Cymbidium ensifolium (L.) Sw. root extracts have and absorb preferably ultraviolet ability.
Two, preparation contains the sunscreen cream of Mohs Cymbidium ensifolium (L.) Sw. root extract
Raw material: tristerin 3g, stearic acid 7g, hexadecanol 5g, vaseline 1g, sodium lauryl sulphate 0.5g, Borax 1.5g, glycerol 3ml, distilled water 80ml, appropriate antiseptic.
Preparation method: tristerin, stearic acid, hexadecanol, vaseline and glycerol are mixed to (oil phase), Borax and water are mixed to (water).Be heated to respectively 90 ℃, make material dissolution even, water keeps 20min sterilizing under 90 ℃.Then, under vigorous stirring, water being joined to oil phase, continue to stir 30min and carry out saponification.When temperature drops to 50 ℃, add antiseptic.Standing, be cooled to room temperature, obtain sunscreen cream substrate.
Get the Mohs Cymbidium ensifolium (L.) Sw. root extract of 0.01g drying, add acetone solution and be dissolved in the 25ml volumetric flask, being made into concentration is the Mohs Cymbidium ensifolium (L.) Sw. root extract solution (referred to as solution A) of 400 μ g/ml, by the listed formula of table 2, prepares sunscreen cream.
Table 2 sunscreen cream formula
Accurately take each sunscreen cream 0.010g, be dissolved in water and be dissolved in the 25ml volumetric flask, take distilled water as blank, at the 200-400nm place, survey respectively its ultraviolet absorption value, the results are shown in Table 3.
Table 3 sunscreen cream absorbs the ultraviolet effect
Result shows, the blank substrate of absorption ultraviolet energy force rate of the sunscreen cream of interpolation Mohs Cymbidium ensifolium (L.) Sw. root extract is good, it is better that the sunscreen cream (sunscreen cream 1) that contains 25% concentration and be the Mohs Cymbidium ensifolium (L.) Sw. root extract of 400 μ g/ml absorbs the ultraviolet ability, near brand product L'Oreal sunscreen cream, absorbs the ultraviolet ability.The sunscreen cream of explanation take Mohs Cymbidium ensifolium (L.) Sw. root extract as sunscreen has obvious sun-proof result.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (6)
1. a Mohs Cymbidium ensifolium (L.) Sw. root extract, is characterized in that getting the pulverizing of Mohs Cymbidium ensifolium (L.) Sw. root and be placed in acetone, at 30 ℃～60 ℃ of temperature, ultrasonic power, is that 75～220W, frequency are under the condition of 25～45 Hz, supersound extraction 20～80min; Filter, filtering residue repeats to extract 2 times, merging filtrate; Filtrate is evaporated to extractum under 40～50 ℃, through lyophilization, obtains Mohs Cymbidium ensifolium (L.) Sw. root extract.
2. Mohs Cymbidium ensifolium (L.) Sw. root extract according to claim 1, it is characterized in that: this extract is in preparation process, and acetone is 40～100mL/g with the volume mass ratio of Mohs Cymbidium ensifolium (L.) Sw. root.
3. the application of Mohs Cymbidium ensifolium (L.) Sw. root extract claimed in claim 1 in preparing sunscreen product.
4. application according to claim 3 is characterized in that: described sunscreen product is sunscreen cream.
5. sunscreen cream that comprises the described Mohs Cymbidium ensifolium (L.) Sw. root of claim 1 extract, it is characterized in that: described sunscreen cream is comprised of sunscreen cream substrate, Mohs Cymbidium ensifolium (L.) Sw. root extract solution, wherein, the concentration of Mohs Cymbidium ensifolium (L.) Sw. root extract solution is 400 μ g/mL, the mass ratio=75:25 of sunscreen cream substrate, Mohs Cymbidium ensifolium (L.) Sw. root extract solution～20.
6. sunscreen cream that comprises the described Mohs Cymbidium ensifolium (L.) Sw. root of claim 1 extract, it is characterized in that: described sunscreen cream is comprised of sunscreen cream substrate, Mohs Cymbidium ensifolium (L.) Sw. root extract solution and water, wherein, the concentration of Mohs Cymbidium ensifolium (L.) Sw. root extract solution is 400 μ g/mL, mass ratio=the 75:25 of sunscreen cream substrate, Mohs Cymbidium ensifolium (L.) Sw. root extract solution～20, wherein in mass ratio the addition of water below 5 umbers.
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