CN102524891B - Dangshen health-care beverage and preparation method thereof - Google Patents

Dangshen health-care beverage and preparation method thereof Download PDF

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CN102524891B
CN102524891B CN201210035217.7A CN201210035217A CN102524891B CN 102524891 B CN102524891 B CN 102524891B CN 201210035217 A CN201210035217 A CN 201210035217A CN 102524891 B CN102524891 B CN 102524891B
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pure water
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fruit
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CN102524891A (en
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胡芳弟
苟于强
杨春霞
安婧
郭凯强
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Lanzhou Foci Pharmaceutical Co ltd
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Lanzhou University
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Abstract

The invention discloses dangshen health-care beverage. According to the formula, every 1000ml of danshen health-care beverage contains 200-100g of fresh dangshen or 5-30g of dried dangshen, 20-100g of fresh hawthorn or 5-30g of dried hawthorn, 5-15g of sweet-scented osmanthus, 0-100g of fresh medlar or 0-25g of dried medlar, 100-800mg of inositol, 0.1-0.8g of taurine, 0-20g of citric acid, 0-25g of sucrose, 0-30g of honey and the balance being pure water. The preparation method of the danshen health-care beverage has the following beneficial effects: 1, since a microwave and water-boiling combined process is adopted for extracting and the microwave extracting time and material/liquid ratio are optimized, the extracting efficiency and the preparation speed are improved greatly, damage to nutrients contained in the product is reduced in the producing process and nutritive components and functional components of raw materials are maintained greatly; 2, since fresh raw materials are used for preparing the beverage, the quality guarantee period of the raw materials is prolonged, the method can be applied to industrial production and the utilization rate of dangshen is improved; and 3, the preparation process is simple, the production cost is low, the prepared health-care beverage has good taste and has good nutritive health-care functions after being taken for a long time, wherein the nutritive health-care functions mainly refer to the effects of tonifying Qi, enriching blood, reduce blood pressure, reducing blood fat, relieving fatigue, refreshing the mind and beautifying.

Description

Dangshen health-care beverage and preparation method thereof
Technical field
The preparation method who the present invention relates to a kind of functional health-care drink, is specifically related to Dangshen health-care beverage and preparation method thereof.
Background technology
Along with people are improving constantly aspect living standard and consumption demand, the functionalization of beverage products, diversified development have been trend of the times.The percentage that China's drinks accounts for soft drink market has risen to 20% left and right for 2008.Huge market potential makes many enterprises fall over each other in recent years to get " function " board, and after red ox, domestic drinks market is that new product is continuous, not only has setting foot in of beverage giant, more has the intervention of capitals outside media business.But it seems now, compare the beverage markets such as mineral water, fruit juice, the integral body in domestic drinks market is high-volume little, and development relatively lags behind, and the whole world per capita annual drinks consumption has reached just 0.5 kilogram, 7 kilograms of ,Er China.Visible drinks also exists sizable market growth space.
The health drink with multiple health care and auxiliary therapeutic action that the Radix Codonopsis hawthorn of also not take is in the market prepared as primary raw material.
Radix Codonopsis, taste is sweet; Property flat, return spleen lung channel, be used for the treatment of weakness of the spleen and the stomach, anorexia and loose stool, limbs fatigue, the deficiency syndrome of the lung breath with cough, breathe hard spontaneous perspiration, thirsty, gas micro-two and lose all cards.Its main effect is tonifying Qi, holds concurrently and can nourish blood simultaneously, and this is its a large feature.Radix Codonopsis has excitation to nervous system, can strengthen Abwehrkraft des Koepers; Can make a rabbit erythrocyte and hemoglobin increase; Can expand peripheral vessels and reduce blood pressure, and can suppress adrenergic boosting; There is Gastrointestinal motility adjustment, antiulcer, gastric acid secretion inhibiting, reduces the effects such as pepsin activity; Also the caused leucocyte of chemotherapy radioactive ray is declined and has castering action.
Hawthorn, contains in every 100 grams of fresh haw berry meat: 89 milligrams of vitamin Cs are only second to fresh jujube, Kiwi berry occupies the 3rd in fruit; Vitamin B2 content is higher 5 times than apple, suitable with banana, and fruit arranged side by side with it is the first; 0.82 gram of carrotene is 10 times of apple; 85 milligrams of calcium are only second to olive among group fruit.In fresh haw berry, the content of vitamin E also occupies the hat of fruit, and it also contains the mineral matters such as rich in protein, amino acid and sodium, potassium, manganese.In addition, in hawthorn, pectin content shelter has first of fruit.Pectin has the effect that reduces cholesterol and blood sugar, prevention gall stone.And according to current research, pectin also has radiation-resistant effect, radioactive element---strontium, cobalt, the palladium etc. that can walk half from band in body.In hawthorn, to can be considered be enteron aisle " street cleaner " to a large amount of dietary fibers.Hawthorn not only has very high nutritive value, also has very high medical value.In hawthorn because contain various active material, as triterpene substance, organic acid, dietary fiber, flavonoids material, Vitexin, therefore have hemangiectasis increase coronary blood flow, improve heart body, softening blood vessel, reduce blood pressure, aid digestion, increase appetite, the having indigestion that disappears, anti-cancer and kill cancer action.Hawthorn also has sterilization and astriction, so doctor also treats the diseases such as bacillary dysentery, enteritis with hawthorn.Drink haw tea every day, just can reach with the passage of time the effect of reducing blood lipid, softening blood vessel.
Matrimony vine property is sweet, flat, returns liver kidney channel, and energy tonifying kidney and benefiting sperm, nourishes the liver to improve visual acuity, and blood-enriching tranquillizing, promotes the production of body fluid to quench thirst, and moistens the lung and relieve the cough.Control liver-kidney Yin deficiency, soreness and weakness of waist and knees, dizziness, dizzy, the many tear of blurred vision, cough due to consumptive disease, quenches one's thirst, seminal emission.Contain carrotene, betaine, vitamin A, vitamin B1, vitamin B2, vitamin C and calcium, phosphorus, iron etc., have and increase the pharmacological action that leucocyte is active, promote liver cell new life, also can hypotensive, hypoglycemic, blood fat.Vitamin C content in matrimony vine is higher than orange, and content beta-carotene is higher than carrot, and iron content is also higher than beefsteak, and matrimony vine also has Yang strengthening function.Matrimony vine, as the good merchantable brand of nourishing of medicine-food two-purpose, all can take throughout the year, can improve physique, is beneficial to sleep.For women, often eat the effect that matrimony vine can also play beauty face-whitening-nourishing.
Sweet osmanthus is warm in nature, and taste is pungent.The intense strain of releiving, often drinks, and can purify body and mind, Rosemary Essence Oil, reaches the effect of refreshing oneself.In addition, also there is the effect that anticancer, clearing heat and detoxicating, expelling wind and clearing away cold, profit spleen are waken up stomach, improved a poor appetite and lose weight.For awake stomach, the cough-relieving of promoting the production of body fluid, reduce phlegm, analgesia, pleasant effectively, can the whole intestines of stomach invigorating, relax the gastrointestinal diseases such as duodenal ulcer and gastroptosis, gaseous distention.And can treat mouth parched and tongue scorched and flatulence, to reach the effect of the moistening lung that takes care of health, elimination digestive discomfort; Can also treat child frightened with the symptom such as stomachache, toothache, halitosis.Often drinking osmanthus flower tea can skin whitening, removes vivotoxin and all night long just.
Summary of the invention
Object of the present invention is exactly for above-mentioned defect of the prior art, provides that a kind of to take Radix Codonopsis, hawthorn be primary raw material, is aided with matrimony vine and sweet osmanthus, the health drink with multiple health care and auxiliary therapeutic action being prepared into.
To achieve these goals, technical scheme provided by the invention is:
Dangshen health-care beverage, described formula is to contain in every 1000ml Dangshen health-care beverage:
Fresh Radix Codonopsis 20-100g or codonopsis pilosula 5-30g, hawthorn fresh fruit 20-100g or hawthorn dry fruit 5-30g, sweet osmanthus 5-15g, medlar fresh fruit 0-100g or dried fruit of lycium barbarum 0-25g; inositol 100-800mg, taurine 0.1-0.8g, citric acid 0-20g; sucrose 0-25g, honey 0-30g, surplus is pure water.
Above-mentioned formula can also be for containing in every 1000ml Dangshen health-care beverage:
Fresh Radix Codonopsis 50-100g, hawthorn fresh fruit 50-100g, sweet osmanthus 5g, medlar fresh fruit 5-25g, inositol 300-600mg, taurine 0.2-0.6g, citric acid 5-10g, sucrose 10-20g, honey 10-25g, surplus is pure water.
Above-mentioned formula can also be for containing in every 1000ml Dangshen health-care beverage:
Codonopsis pilosula 20-30g, hawthorn dry fruit 20-50g, sweet osmanthus 5-10g, dried fruit of lycium barbarum 0-25g, inositol 400-600mg, taurine 0.5-0.6g, citric acid 8-10g, sucrose 15-20g, honey 15-25g, surplus is pure water.
Another object of the present invention has been to provide the preparation method of above-mentioned Dangshen health-care beverage, includes following steps:
1) fresh Radix Codonopsis, hawthorn dry fruit and dried fruit of lycium barbarum are taken according to formula, and added formula ratio sweet osmanthus after 90-130 ℃ of steam treatment 5-30 minute, obtain mixed material; Or
Codonopsis pilosula, hawthorn fresh fruit and medlar fresh fruit are directly mixed with formula ratio sweet osmanthus, obtain mixed material;
2) by step 1) add the pure water that is equivalent to mixed material 1-3 times quality in the mixed material that obtains, and carry out Microwave Extraction, the micro-wave oven Microwave Extraction 5-15 minute that extraction conditions is 200-800W, obtains preliminary treatment compound;
3) by step 2) the preliminary treatment compound that obtains is placed in cooker and carries out boiling, and fills into pure water to being equivalent to mixed material 5-10 times quality, and decoct after 40-120 minute 80-100 mesh sieve and filter and obtain filtrate one and material slag;
4) by step 3) the material slag that obtains is placed in cooker and adds and be equivalent to decoct 30-60 minute after mixed material 5-8 pure water doubly, filters and obtains filtrate two, and filtering order number is 80-100 order;
5) by step 3) filtrate one and the step 4 that obtain) filtrate two mixing that obtain, obtain total extract;
6) taurine and inositol are taken according to formula, join step 5 after adding a small amount of dissolved in purified water) in total extract of obtaining, after stirring, add again sucrose, citric acid and the honey of formula ratio, then filter 80-100 order; Again filtrate is settled to 1000ml with pure water, obtains two stage treatment compound;
7) by step 6) the two stage treatment compound that obtains is at pressure 40-50MPa, temperature 40-80 ℃ is carried out high pressure homogenizing and processes after 1-2 time, in power 100-1500W, in the Ultrasound Instrument of frequency 40-100KHz, carry out ultrasonic degas 30-120 second, high-temperature sterilization 5-20s, sterilising temp is 138-150 ℃, obtains health drink semi-finished product;
8) by step 7) obtain health drink semi-finished product and carry out filling, check and packing, obtain the finished product of Dangshen health-care beverage.
The preparation method of above-mentioned Dangshen health-care beverage, includes following steps:
1) hawthorn fresh fruit is cleaned to rear section, thickness is 1-5mm, and codonopsis pilosula is cleaned rear segment, and length is 1-3cm, after codonopsis pilosula, hawthorn fresh fruit and sweet osmanthus are mixed with formula ratio, obtains mixed material;
2) add and work as in the pure water of 2 times of amounts of raw material gross weight, and carry out Microwave Extraction, the micro-wave oven Microwave Extraction that extraction conditions is 500W 10 minutes, obtains preliminary treatment compound;
3) by step 2) the preliminary treatment compound that obtains is placed in cooker and carries out boiling, and fills into pure water to being equivalent to 5 times of quality of mixed material, and decoct after 60 minutes 80-100 mesh sieve and filter and obtain filtrate one and material slag;
4) by step 3) the material slag that obtains is placed in after cooker adds the pure water that is equivalent to 5 times of mixed materials and decocts 40 minutes, filters and obtains filtrate two, and filtering order number is 80-100 order;
5) by step 3) filtrate one and the step 4 that obtain) filtrate two mixing that obtain, obtain total extract;
6) taurine and inositol are taken according to formula, after adding a small amount of dissolved in purified water, join step 5) in total extract of obtaining, the sucrose, citric acid and the honey that after stirring, add again formula ratio, then filter, filtering order number is 80-100 order, again filtrate is settled to 1000ml with pure water, obtains two stage treatment compound;
7) by step 6) the two stage treatment compound that obtains is at pressure 45MPa, and 80 ℃ of temperature are carried out high pressure homogenizing and are processed after 2 times, in power 100W, in the Ultrasound Instrument of frequency 40KHz, carry out ultrasonic degas 60 seconds, high-temperature sterilization 5s, sterilising temp is 140-150 ℃, obtains health drink semi-finished product;
8) by step 7) obtain health drink semi-finished product and carry out filling, check and packing, obtain the finished product of Dangshen health-care beverage.
The preparation method of above-mentioned Dangshen health-care beverage, includes following steps:
1) fresh Radix Codonopsis, hawthorn dry fruit and dried fruit of lycium barbarum are taken according to formula, and after 25 minutes, add formula ratio sweet osmanthus in 100 ℃ of steam treatment, obtain mixed material; Or
Codonopsis pilosula, hawthorn fresh fruit and medlar fresh fruit are directly mixed with formula ratio sweet osmanthus, obtain mixed material;
2) add and work as in the pure water of 2 times of amounts of raw material gross weight, and carry out Microwave Extraction, the micro-wave oven Microwave Extraction 5-15 minute that extraction conditions is 500W, obtains preliminary treatment compound;
3) by step 2) the preliminary treatment compound that obtains is placed in cooker and carries out boiling, and fills into pure water to being equivalent to mixed material 5-8 times quality, and decoct after 40-120 minute 80-100 mesh sieve and filter and obtain filtrate one and material slag;
4) by step 3) the material slag that obtains is placed in cooker and adds and be equivalent to decoct 30-60 minute after mixed material 5-6 pure water doubly, filters and obtains filtrate two, and filtering order number is 80-100 order;
5) by step 3) filtrate one and the step 4 that obtain) filtrate two mixing that obtain, obtain total extract;
6) taurine and inositol are taken according to formula, after adding a small amount of dissolved in purified water, join step 5) in total extract of obtaining, the sucrose, citric acid and the honey that after stirring, add again formula ratio, then filter, filtering order number is 80-100 order, again filtrate is settled to 1000ml with pure water, obtains two stage treatment compound;
7) by step 6) the two stage treatment compound that obtains is at pressure 40-50MPa, 65 ℃ of temperature are carried out high pressure homogenizing and are processed after 2 times, in power 500W, in the Ultrasound Instrument of frequency 60KHz, carry out ultrasonic degas 60 seconds, high-temperature sterilization 20-30min, sterilising temp is 121 ℃, obtains health drink semi-finished product;
8) by step 7) obtain health drink semi-finished product and carry out filling, check and packing, obtain the finished product of Dangshen health-care beverage.
The preparation method of above-mentioned Dangshen health-care beverage, includes following steps:
1) fresh Radix Codonopsis, hawthorn dry fruit and dried fruit of lycium barbarum are taken according to formula, and after 25 minutes, add formula ratio sweet osmanthus in 92 ℃ of steam treatment, obtain mixed material; Or
Codonopsis pilosula, hawthorn fresh fruit and medlar fresh fruit are directly mixed with formula ratio sweet osmanthus, obtain mixed material;
2) add and work as in the pure water of 3 times of amounts of raw material gross weight, and carry out Microwave Extraction, the micro-wave oven Microwave Extraction that extraction conditions is 600-800W 8 minutes, obtains preliminary treatment compound;
3) by step 2) the preliminary treatment compound that obtains is placed in cooker and carries out boiling, and fills into pure water to being equivalent to 8 times of quality of mixed material, and decoct after 120 minutes 80-100 mesh sieve and filter and obtain filtrate one and material slag;
4) by step 3) the material slag that obtains is placed in after cooker adds the pure water that is equivalent to 6 times of mixed materials and decocts 60 minutes, filters and obtains filtrate two, and filtering order number is 80-100 mesh sieve;
5) by step 3) filtrate one and the step 4 that obtain) filtrate two mixing that obtain, obtain total extract;
6) taurine and inositol are taken according to formula, after adding a small amount of dissolved in purified water, join step 5) in total extract of obtaining, the sucrose, citric acid and the honey that after stirring, add formula ratio, then filter again, and filtering order number is 80-100 mesh sieve; Again filtrate is settled to 1000ml with pure water, obtains two stage treatment compound;
7) by step 6) the two stage treatment compound that obtains is at pressure 40-50MPa, and 55 ℃ of temperature are carried out high pressure homogenizing and are processed after 2 times, in power 1000W, in the Ultrasound Instrument of frequency 60KHz, carry out ultrasonic degas 45 seconds, high-temperature sterilization 10s, sterilising temp is 140 ℃, obtains health drink semi-finished product;
8) by step 7) obtain health drink semi-finished product and carry out filling, check and packing, obtain the finished product of Dangshen health-care beverage.。
Beneficial effect of the present invention is:
The present invention is in conjunction with Traditional Chinese medical theory and modern scientific theory, take Radix Codonopsis, hawthorn to be prepared from as primary raw material, and the present invention compared with prior art has following advantage:
1) the present invention adopts microwave to extract in conjunction with the technique of poach, and the time of Microwave Extraction and solid-liquid ratio are optimized, extraction efficiency and manufacturing speed improve greatly, and reduced the destruction of process to the contained nutritional labeling of product, retained greatly nutrition and the functional component of raw material.
2) another advantage of the present invention is to adopt fresh feed processing to prepare beverage, has extended the shelf-life of raw material, and the method can realize suitability for industrialized production, has improved the resource utilization of Radix Codonopsis.
3) preparation technology of the present invention is simple, production cost is low, and the health drink mouthfeel making is good, and long-term drinking has very strong nutrition health-care functions.Main manifestations is tonifying Qi, nourishes blood, hypotensive, reducing blood lipid, fatigue-relieving, the effects such as beauty treatment of refreshing oneself.
The specific embodiment
Embodiment 1:
The formula of the present embodiment Dangshen health-care beverage is that every 1000ml beverage contains:
Fresh Radix Codonopsis 50g, hawthorn fresh fruit 50g, sweet osmanthus 5g, medlar fresh fruit 25g, inositol 400mg, taurine 0.3g, citric acid 10g, sucrose 10g, honey 15g, surplus is pure water.
The present embodiment Dangshen health-care beverage production technology is as follows:
Ripe hawthorn fresh fruit is cleaned, and is cut into 1-5mm thin slice standby, and fresh codonopsis pilosula is cleaned, and is cut into 1-3cm segment standby.By formula, take the fresh medicinal material of clean Radix Codonopsis, hawthorn fresh fruit, sweet osmanthus, adds and is equivalent to 2 times of amount pure water of total raw material, the ultrasonic extraction of microwave 500W 10 minutes, is then placed in cooker and carries out, and supplementary pure water is to being equivalent to 5 times of amounts of raw material, decoct 60 minutes, filter, obtain filtrate.The dregs of a decoction add the pure water that is equivalent to 5 times of amounts of raw material again, decoct 40 minutes, filter, and obtain filtrate, merge filtrate twice, obtain total extract.
By formula, take taurine (Wuhan City Yuan Cheng development in science and technology Co., Ltd; 694195) and inositol (Hebei Yu Wei food formulation Co., Ltd; 102713531116); with after a small amount of dissolved in purified water, add in above-mentioned total extract, stir; sucrose, citric acid, the honey that takes again formula ratio adds and in extract, carries out local flavor adjustment; be adjusted to after best flavor, filter, filtrate is settled to 1000ml with pure water.Filtrate after constant volume is at pressure 45MPa, and 80 ℃ of temperature are carried out high pressure homogenizing and processed after 2 times, in power 100W, and ultrasonic degas 1min in 40KHz Ultrasound Instrument, high-temperature sterilization 5s, sterilising temp is 140-150 ℃.Filling, check, packing gets product.
Embodiment 2:
The formula of the present embodiment Dangshen health-care beverage is that every 1000ml beverage contains:
Fresh Radix Codonopsis 50g or codonopsis pilosula 20g, hawthorn fresh fruit 50g or hawthorn dry fruit 20g, sweet osmanthus 5-15g, medlar fresh fruit 25g or dried fruit of lycium barbarum 10g, inositol 500mg, taurine 0.5g, citric acid 8g, sucrose 15g, honey 20g, surplus is pure water.By formula, take Radix Codonopsis, hawthorn, matrimony vine, clean, 100 ℃ of steam treatment 25 minutes, the fresh medicinal material of Radix Codonopsis, hawthorn fresh fruit, medlar fresh fruit if, can not carry out steam treatment, take subsequently formula ratio sweet osmanthus and mix with above-mentioned raw materials, add and be equivalent to 2 times of amount pure water of total raw material, microwave 500W extracts 5-15 minute, then be placed in cooker and carry out, supplementary pure water is doubly measured to being equivalent to raw material 5-8, decocts 40-120 minute, filter, obtain filtrate.The dregs of a decoction add and are equivalent to the pure water that raw material 5-6 doubly measures, and decoct 30-60 minute, filter, and obtain filtrate, merge filtrate twice, obtain total extract.
By formula, take taurine and inositol, with after a small amount of dissolved in purified water, add in above-mentioned total extract; stir, then the sucrose, citric acid, the honey that take formula ratio adds in extract and carry out local flavor adjustment, be adjusted to after best flavor; filter, filtrate is settled to 1000ml with pure water.Filtrate after constant volume is at pressure 40-50MPa, and 65 ℃ of temperature are carried out high pressure homogenizing and processed after 2 times, in power 500W, carry out ultrasonic degas 60 seconds in the Ultrasound Instrument of frequency 60KHz, high-temperature sterilization 20-30min, and sterilising temp is 121 ℃.Filling, check, packing gets product.
Embodiment 3:
The formula of the present embodiment Dangshen health-care beverage is that every 1000ml beverage contains:
Codonopsis pilosula 20g, hawthorn dry fruit 20g, sweet osmanthus 5g, dried fruit of lycium barbarum 10g, inositol 600mg, taurine 0.5g, citric acid 10g, sucrose 20g, honey 25g, surplus is pure water.By formula, take Radix Codonopsis, hawthorn, matrimony vine, clean, 92 ℃ of steam treatment 25 minutes, take subsequently formula ratio sweet osmanthus and mix with above-mentioned raw materials, add and are equivalent to 3 times of amount pure water of total raw material, microwave 600-800W extracts 8 minutes, then be placed in cooker and carry out, supplementary pure water, to being equivalent to 8 times of amounts of raw material, decocts 120 minutes, filter, obtain filtrate.The dregs of a decoction add the pure water that is equivalent to 6 times of amounts of raw material again, decoct 60 minutes, filter, and obtain filtrate, merge filtrate twice, obtain total extract.
By formula, take taurine and inositol, with after a small amount of dissolved in purified water, add in above-mentioned total extract; stir, then the sucrose, citric acid, the honey that take formula ratio adds in extract and carry out local flavor adjustment, be adjusted to after best flavor; filter, filtrate is settled to 1000ml with pure water.Filtrate after constant volume is at pressure 40-50MPa, and 55 ℃ of temperature are carried out high pressure homogenizing and processed after 2 times, in power 1000W, carry out ultrasonic degas 45 seconds in the Ultrasound Instrument of frequency 60KHz, high-temperature sterilization 10s, and sterilising temp is 140 ℃.Filling, check, packing gets product.
Embodiment 4:
1. Dangshen health-care beverage alleviating physical fatigue test of the present invention
1.1 materials and methods
1.1.1 sample Dangshen health-care beverage raw sample is brown liquid, density is about 1g/ml.Sample is taken out after sucrose without effect, citric acid, concentrated 20 times of atmospheric evaporation, concentrate (density is about 1.2g/ml) is as tested material.
1.1.2 40 of Kunming mouses are male, body weight 18-22g, regular grade.Be purchased from Lanzhou University's Experimental Animal Center.Be divided into immediately 4 groups, carry out respectively swimming with a load attached to the body experiment, blood Plasma lactate.
1.1.3 dosage is respectively 5 times, 10 times, 20 times of human body RD, i.e. 30g/kg, and 60g/kg, 120g/kg, separately establishes blank group, gives the physiological saline of same volume.Above four groups of continuous gastric infusions are measured corresponding index after 30 days.
1.1.4 key instrument and reagent: ADVIA1200 type automatic clinical chemistry analyzer, lactic acid analyzer, swimming trunk.
1.1.5 experimental technique
1.1.5.1 swimming with a load attached to the body experiment: mouse is given with tested material and physiological saline after 1 hour in last, make the bear a heavy burden lead flake of 5% body weight of afterbody, the self-control swimming trunk went swimming that is no less than 25 ± 5 ℃ of 30cm, water temperature in the depth of water, records mouse and starts to the death time from swimming.
1.1.2.2 blood Plasma lactate: mouse gives tested material after 1 hour in last, posterior orbit muscle arteries and veins is from getting blood 20ul in dilution, with lactate analysis instrument, measure Serum lactic acid content, then make it at depth of water 30cm, the swimming trunk went swimming 10min that water temperature is 25 ℃, after swimming, after 0,20min, in posterior orbit muscle arteries and veins clump, get blood 20ul in dilution respectively, carry out Serum lactic acid content mensuration.
1.1.6 with SPSSll.0 statistical software, carry out data analysis, data all represent with x ± s, and measurement data is first through normal distribution and homogeneity test of variance, then adopt variance analysis and t check.
1.2 experimental result
1.2.1 the impact of Dangshen health-care beverage on the mice burden swimming time is as shown in table 1:
Table 1
Dosage g/kg Size of animal (only) The swimming with a load attached to the body time (min) P value
Blank group 10 30.1±11.3
30 10 46.2±9.7 0.412
60 10 50.3±14.7 0.015
120 10 59.6±10.1 0.001
From table 1, middle dosage and high dose group mice burden swimming time are obviously longer than control group, with blank group comparing difference remarkable (p < 0.05), to point out under this experiment condition, Dangshen health-care beverage can extend the mice burden swimming time.
1.2.3 Dangshen health-care beverage on mouse movement after the impact of blood lactate level, as shown in table 2:
Blood lactic acid TG-AUC=1/2 * (the rear 0min blood Lactate of blood Lactate+swimming before swimming) * 10+1/2 * (20min blood Lactate after the rear 0min blood Lactate+swimming of swimming) * 20.
Table 2
Dosage g/kg Size of animal (only) Blood lactic acid TG-AUC P value
Blank group 10 148.4±19.1
30 10 137.7±12.8 0.512
60 10 132.9±19.1 0.031
120 10 110.5±16.7 0.001
From table 2, middle dosage and high dose group Mouse Blood lactic acid TG-AUC are significantly less than control group, with control group comparing difference remarkable (p < 0.05), to point out under this experiment condition, Dangshen health-care beverage can reduce Mouse Blood lactic acid TG-AUC.
2. Dangshen health-care beverage reducing blood lipid test of the present invention
2.1 materials and methods
2.1.1 animal male SD rat, cleans level, and body weight (150 ± 10) g, is purchased from Lanzhou University's Experimental Animal Center.
2.1.2 medicine and reagent: sample Dangshen health-care beverage raw sample is brown liquid, and density is about 1g/ml.Sample is taken out after sucrose without effect, citric acid, concentrated 20 times of atmospheric evaporation, concentrate (density is about 1.2g/ml) is as tested material.Gastric infusion, faces fresh preparation of used time.Serum total cholesterol (TC), triglycerides (TG), HDL-C (HDL-C) and LDL-C (LDL-C) mensuration kit build up reagent company by Nanjing to be provided.The preparation of high lipid food: formulated with cholate 0.2%, lard 8%, propylthiouracil (PTU) 0.2%, cholesterol 2%, basal feed 89.6%.
2.1.3 Animal Model adapt to be fed after 1W with grouping rat, is divided at random 6 groups, 10 every group, is respectively blank group, hyperlipidemia model Zu, tested material senior middle school low dose group (15,30,60g/kg).Blank group feeding standard feed, all the other are respectively organized the high lipid food of all feeding and make hyperlipemia animal model, and the 4W that feeds continuously measures blood lipids index, to confirm modeling success or not.After modeling success, start gastric infusion, gavage volume 10ml/kg, the basic, normal, high dosage group of tested material is the tested material solution of gavage 15,30,60g/kg concentration respectively, blank group and hyperlipidemia model group gavage isometric(al) distilled water, every day, gavage was 1 time, respectively organizes rat and all freely ingest and drink water during gavage.
2.1.4 experimental index is determined at before and after the animal model of 4W, respectively to Rat Septal curfew food, and etherization, eye socket rear vein beard is got blood, measures TC, TG, for model, detects.After gastric infusion certain hour, by Rat Septal curfew food, eye socket rear vein beard is got blood, and separation of serum is surveyed serum TC, TG, HDL, LDL, and indices is all undertaken by kit operation instruction, and every gastric infusion 2W blood sampling 1 time, detects each index.
2.1.5 statistical method adopts SPSSll.0 statistical software to carry out data analysis, and data all represent with i ± s, and measurement data is first through normal distribution and homogeneity test of variance, then adopts variance analysis and t check.
2.2 experimental result
2.2.1 the impact of Dangshen health-care beverage on high fat Serum TC, TG, as shown in table 3:
Table 3
Figure BDA0000136033430000081
Figure BDA0000136033430000091
With the comparison of blank group: * represents P < 0.01; With model group comparison: arepresent P < 0.01.
2.2.2 the impact of Dangshen health-care beverage on high fat rat blood serum LDL-C, HDL-C, as shown in table 4:
Table 4
Figure BDA0000136033430000092
With the comparison of blank group: * represents P < 0.01; Represent P < 0.01 with model group comparison: a.
By table 3, table 4 prompting, under this experiment condition, Dangshen health-care beverage has inhibitory action to rat high fat of blood.
3. the dyspeptic experiment of couple general population:
Select tested number 25 people's indigestion crowds, Application Example 3 health drink 30 days, the amount of drinking is 250-500ml every day left and right, observes 30 days, effective 20 people, substantially invalid 3 people, total effective rate reaches 80%.With embodiment 1 and 2, carry out same experiment, effect is identical with embodiment 3.
4. the hypotensive experiment of couple general population:
Tested crowd is the crowd with hypertension history, and tested number 30 people, show as at ordinary times and take antihypertensive drugs, quote embodiment 3 health drink 30 days, the amount of drinking is 250-500ml every day left and right, observes 30 days, effective 25 people, substantially invalid 3 people, total effective rate reaches 83%.With embodiment 1 and 2, carry out same experiment, effect is identical with embodiment 3.
Finally it should be noted that: the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although the present invention is had been described in detail with reference to previous embodiment, for a person skilled in the art, its technical scheme that still can record aforementioned each embodiment is modified, or part technical characterictic is wherein equal to replacement.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.

Claims (6)

1. the preparation method of Dangshen health-care beverage, it is characterized in that, every 1000ml Dangshen health-care beverage is made by following raw material: fresh Radix Codonopsis 20-100g or codonopsis pilosula 5-30g, hawthorn fresh fruit 20-100g or hawthorn dry fruit 5-30g, sweet osmanthus 5-15g, medlar fresh fruit 0-100g or dried fruit of lycium barbarum 0-25g, inositol 100-800mg, taurine 0.1-0.8g, citric acid 0-20g, sucrose 0-25g, honey 0-30g, surplus is pure water;
Preparation method includes following steps:
1) fresh Radix Codonopsis, hawthorn dry fruit and dried fruit of lycium barbarum are taken according to formula, and added formula ratio sweet osmanthus after 90-130 ℃ of steam treatment 5-30 minute, obtain mixed material; Or
Codonopsis pilosula, hawthorn fresh fruit and medlar fresh fruit are directly mixed with formula ratio sweet osmanthus, obtain mixed material;
2) in mixed material step 1) being obtained, add the pure water that is equivalent to mixed material 1-3 times quality, and carry out Microwave Extraction, the micro-wave oven Microwave Extraction 5-15 minute that extraction conditions is 200-800W, obtains preliminary treatment compound;
3) by step 2) the preliminary treatment compound that obtains is placed in cooker and decocts, and fills into pure water to being equivalent to mixed material 5-10 times quality, and decoct after 40-120 minute 80-100 mesh sieve and filter and obtain filtrate one and material slag;
4) material slag step 3) being obtained is placed in after cooker adds the pure water that is equivalent to mixed material 5-8 times and decocts 30-60 minute, and 80-100 mesh sieve filters and obtains filtrate two;
5) filtrate two mixing that the filtrate one step 3) being obtained and step 4) obtain, obtain total extract;
6) taurine and inositol are taken according to formula, add and join after a small amount of dissolved in purified water in total extract that step 5) obtains, the sucrose, citric acid and the honey that after stirring, add again formula ratio, then 80-100 mesh sieve filters, again filtrate is settled to 1000ml with pure water, obtains two stage treatment compound;
7) two stage treatment compound step 6) being obtained is at pressure 40-50MPa, temperature 40-80 ℃ is carried out high pressure homogenizing and processes after 1-2 time, in power 100-1500W, in the Ultrasound Instrument of frequency 40-100KHz, carry out ultrasonic degas 30-120 second, high-temperature sterilization 5-20s, sterilising temp is 138-150 ℃, obtains health drink semi-finished product;
8) step 7) is obtained to health drink semi-finished product and carry out filling, check and packing, obtain the finished product of Dangshen health-care beverage.
2. the preparation method of Dangshen health-care beverage, it is characterized in that, every 1000ml Dangshen health-care beverage is made by following raw material: fresh Radix Codonopsis 20-100g or codonopsis pilosula 5-30g, hawthorn fresh fruit 20-100g or hawthorn dry fruit 5-30g, sweet osmanthus 5-15g, medlar fresh fruit 0-100g or dried fruit of lycium barbarum 0-25g, inositol 100-800mg, taurine 0.1-0.8g, citric acid 0-20g, sucrose 0-25g, honey 0-30g, surplus is pure water;
Preparation method includes following steps:
1) hawthorn fresh fruit is cleaned to rear section, thickness is 1-5mm, and codonopsis pilosula is cleaned rear segment, and length is 1-3cm, after codonopsis pilosula, hawthorn fresh fruit and sweet osmanthus are mixed with formula ratio, obtains mixed material;
2) add and work as in the pure water of 2 times of amounts of raw material gross weight, and carry out Microwave Extraction, the micro-wave oven Microwave Extraction that extraction conditions is 500W 10 minutes, obtains preliminary treatment compound;
3) by step 2) the preliminary treatment compound that obtains is placed in cooker and carries out boiling, and fills into pure water to being equivalent to 5 times of quality of mixed material, and decoct after 60 minutes 80-100 mesh sieve and filter and obtain filtrate one and material slag;
4) material slag step 3) being obtained is placed in after cooker adds the pure water that is equivalent to 5 times of mixed materials and decocts 40 minutes, filters and obtains filtrate two, and filtering order number is 80-100 order;
5) filtrate two mixing that the filtrate one step 3) being obtained and step 4) obtain, obtain total extract;
6) taurine and inositol are taken according to formula, add and join after a small amount of dissolved in purified water in total extract that step 5) obtains, the sucrose, citric acid and the honey that after stirring, add again formula ratio, then filter, filtering order number is 80-100 order, again filtrate is settled to 1000ml with pure water, obtains two stage treatment compound;
7) two stage treatment compound step 6) being obtained is at pressure 45MPa, and 80 ℃ of temperature are carried out high pressure homogenizing and processed after 2 times, in power 100W, in the Ultrasound Instrument of frequency 40KHz, carry out ultrasonic degas 60 seconds, high-temperature sterilization 5s, sterilising temp is 140-150 ℃, obtains health drink semi-finished product;
8) step 7) is obtained to health drink semi-finished product and carry out filling, check and packing, obtain the finished product of Dangshen health-care beverage.
3. the preparation method of Dangshen health-care beverage, it is characterized in that, every 1000ml Dangshen health-care beverage is made by following raw material: fresh Radix Codonopsis 20-100g or codonopsis pilosula 5-30g, hawthorn fresh fruit 20-100g or hawthorn dry fruit 5-30g, sweet osmanthus 5-15g, medlar fresh fruit 0-100g or dried fruit of lycium barbarum 0-25g, inositol 100-800mg, taurine 0.1-0.8g, citric acid 0-20g, sucrose 0-25g, honey 0-30g, surplus is pure water;
Preparation method includes following steps:
1) fresh Radix Codonopsis, hawthorn dry fruit and dried fruit of lycium barbarum are taken according to formula, and after 25 minutes, add formula ratio sweet osmanthus in 100 ℃ of steam treatment, obtain mixed material; Or
Codonopsis pilosula, hawthorn fresh fruit and medlar fresh fruit are directly mixed with formula ratio sweet osmanthus, obtain mixed material;
2) add and work as in the pure water of 2 times of amounts of raw material gross weight, and carry out Microwave Extraction, the micro-wave oven Microwave Extraction 5-15 minute that extraction conditions is 500W, obtains preliminary treatment compound;
3) by step 2) the preliminary treatment compound that obtains is placed in cooker and carries out boiling, and fills into pure water to being equivalent to mixed material 5-8 times quality, and decoct after 40-120 minute 80-120 mesh sieve and filter and obtain filtrate one and material slag;
4) material slag step 3) being obtained is placed in after cooker adds the pure water that is equivalent to mixed material 5-6 times and decocts 30-60 minute, filters and obtains filtrate two, and filtering order number is 80-120 order;
5) filtrate two mixing that the filtrate one step 3) being obtained and step 4) obtain, obtain total extract;
6) taurine and inositol are taken according to formula, add and join after a small amount of dissolved in purified water in total extract that step 5) obtains, the sucrose, citric acid and the honey that after stirring, add again formula ratio, then filter, filtering order number is 80-120 order, again filtrate is settled to 1000ml with pure water, obtains two stage treatment compound;
7) two stage treatment compound step 6) being obtained is at pressure 40-50MPa, and 65 ℃ of temperature are carried out high pressure homogenizing and processed after 2 times, in power 500W, in the Ultrasound Instrument of frequency 60KHz, carry out ultrasonic degas 60 seconds, high-temperature sterilization 20-30min, sterilising temp is 121 ℃, obtains health drink semi-finished product;
8) step 7) is obtained to health drink semi-finished product and carry out filling, check and packing, obtain the finished product of Dangshen health-care beverage.
4. the preparation method of Dangshen health-care beverage, it is characterized in that, every 1000ml Dangshen health-care beverage is made by following raw material: fresh Radix Codonopsis 20-100g or codonopsis pilosula 5-30g, hawthorn fresh fruit 20-100g or hawthorn dry fruit 5-30g, sweet osmanthus 5-15g, medlar fresh fruit 0-100g or dried fruit of lycium barbarum 0-25g, inositol 100-800mg, taurine 0.1-0.8g, citric acid 0-20g, sucrose 0-25g, honey 0-30g, surplus is pure water;
Preparation method includes following steps:
1) fresh Radix Codonopsis, hawthorn dry fruit and dried fruit of lycium barbarum are taken according to formula, and after 25 minutes, add formula ratio sweet osmanthus in 92 ℃ of steam treatment, obtain mixed material; Or
Codonopsis pilosula, hawthorn fresh fruit and medlar fresh fruit are directly mixed with formula ratio sweet osmanthus, obtain mixed material;
2) add and work as in the pure water of 3 times of amounts of raw material gross weight, and carry out Microwave Extraction, the micro-wave oven Microwave Extraction that extraction conditions is 600-800W 8 minutes, obtains preliminary treatment compound;
3) by step 2) the preliminary treatment compound that obtains is placed in cooker and carries out boiling, and fills into pure water to being equivalent to 8 times of quality of mixed material, and decoct after 120 minutes 80-100 mesh sieve and filter and obtain filtrate one and material slag;
4) material slag step 3) being obtained is placed in after cooker adds the pure water that is equivalent to 6 times of mixed materials and decocts 60 minutes, filters and obtains filtrate two, and filtering order number is 80-100 order;
5) filtrate two mixing that the filtrate one step 3) being obtained and step 4) obtain, obtain total extract;
6) taurine and inositol are taken according to formula, add and join after a small amount of dissolved in purified water in total extract that step 5) obtains, the sucrose, citric acid and the honey that after stirring, add again formula ratio, then filter, filtering order number is 80-120 order, again filtrate is settled to 1000ml with pure water, obtains two stage treatment compound;
7) two stage treatment compound step 6) being obtained is at pressure 40-50MPa, and 55 ℃ of temperature are carried out high pressure homogenizing and processed after 2 times, in power 1000W, in the Ultrasound Instrument of frequency 60KHz, carry out ultrasonic degas 45 seconds, high-temperature sterilization 10s, sterilising temp is 140 ℃, obtains health drink semi-finished product;
8) step 7) is obtained to health drink semi-finished product and carry out filling, check and packing, obtain the finished product of Dangshen health-care beverage.
5. according to the preparation method of the arbitrary described Dangshen health-care beverage of claim 1-4; it is characterized in that, every 1000ml Dangshen health-care beverage is made by following raw material: fresh Radix Codonopsis 50-100g, hawthorn fresh fruit 50-100g; sweet osmanthus 5g; medlar fresh fruit 5-25g, inositol 300-600mg, taurine 0.2-0.6g; citric acid 5-10g; sucrose 10-20g, honey 10-25g, surplus is pure water.
6. according to the preparation method of the arbitrary described Dangshen health-care beverage of claim 1-4; it is characterized in that, every 1000ml Dangshen health-care beverage is made by following raw material: codonopsis pilosula 20-30g, hawthorn dry fruit 20-50g; sweet osmanthus 5-10g; dried fruit of lycium barbarum 0-25g, inositol 400-600mg, taurine 0.5-0.6g; citric acid 8-10g; sucrose 15-20g, honey 15-25g, surplus is pure water.
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