CN109757639A - A kind of ginseng ternary peptide beverage and preparation method thereof - Google Patents
A kind of ginseng ternary peptide beverage and preparation method thereof Download PDFInfo
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Abstract
The present invention provides a kind of ginseng ternary peptide beverage and preparation method thereof, belongs to method for preparing beverage field.The beverage in parts by weight, is prepared from the following raw materials: 5-15 parts of ginseng, 20-50 parts of quinoa, 20-30 parts of jujube, 20-30 parts of fructus lycii, 20-30 parts of cyperue esculentus.The present invention also provides a kind of preparation methods of ginseng ternary peptide beverage.Product of the invention, which has, improves immunity of organisms, adjusts the effect of body's immunity.
Description
Technical field
The invention belongs to method for preparing beverage fields, and in particular to a kind of ginseng ternary peptide beverage and preparation method thereof.
Background technique
With the development of science and technology, the improvement of people's living standards, also constantly improves the nutrient requirement of food, slightly
Food nutrition is no longer satisfied pursuit of the modern people to nutriment, so that food is to more refining the more professional molecule epoch
Continuous development.And in allegro life now, the absorption function of people is also declining, atmosphere pollution, water pollution, chemistry poison
Object radiates, and competition, life and work pressure etc. makes majority be in sub-health state, causes human body to lack nutrition, loses
Many instincts are resisted outer so supplemented with exogenous bioactivity nutriment and life base substance, adjust human health situation
It encroaches on, reduces and prevent disease, creation healthy living will at certainty for people's lives.
Base substance of the protein as living matter basis, into after human body, it is necessary to by the various enzymes of digestive system
Digesting into small-molecular peptides or amino acid can just be absorbed by the body utilization.
The sharp Field doctor of United States Biochemical person's U.S. has found the effect of " active peptides " and active peptides within 1984,
He in obtaining Nobel chemistry Prize for the year.The discovery of active peptides medically defeats various communicable diseases, slow to the mankind
Property disease has started the new situation of cell therapy, gene repair.Scientists find that active peptides are in addition to having one in an experiment
As protein trophism outside, also there is very important irreplaceable adjustment effect to human body, this effect almost relates to
And all physiological activities to human body, such as: nerve, digestion, absorption, metabolism, circulation, growth, reproduction, endocrine etc..Peptide is made
Information is transmitted for neurotransmitter, each system of human body, organ, tissue is allowed to play respective and mass action.It can repair, activate,
Nourishing grows cell, it with synthetic cell, can influence the functional activity of cell again and act on and stimulate human body is important to be immunized
Cell, the i.e. ability of macrophage phagocytic tumour cell.
Existing many raw materials such as ginseng, fructus lycii and jujube etc., are exactly the health care of putative integration of drinking and medicinal herbs since ancient times
It is preferred that these products when directly edible many nutritional ingredients be not easy to be absorbed by human body cell, mass efficient is at branch by human body
Metabolism is gone out.
Summary of the invention
The object of the present invention is to provide a kind of ginseng ternary peptide beverage and preparation method thereof, which is pale yellow powder,
With the distinctive fragrance of ginseng, clean taste, flavour is light palatable with smell, acceptable strong, and has and improve immunity, mends
Spleen benefit lung, tranquilize the mind and promote the intelligence, antitumor, hypoglycemic, reduction cholesterol, anti-aging, antifatigue, blood-enriching face-nourishing and other effects.
Present invention firstly provides a kind of ginseng ternary peptide beverages to be prepared from the following raw materials in parts by weight:
5-15 parts of ginseng, 20-50 parts of quinoa, 20-30 parts of jujube, 20-30 parts of fructus lycii, 20-30 parts of cyperue esculentus.
Preferably, the ginseng ternary peptide beverage is prepared from the following raw materials in parts by weight:
8-10 parts of ginseng, 30-40 parts of quinoa, 22-28 parts of jujube, 22-28 parts of fructus lycii, 22-28 parts of cyperue esculentus.
Preferably, the ginseng ternary peptide beverage is liquid or solid.
The pharmacological action of each raw material of the present invention are as follows:
Ginseng: slightly warm in nature sweet and slightly bitter taste, returns spleen, lung, the heart, kidney channel.With replenish vital energy, restore pulse, and strengthen and remove vital energy, reinforce the spleen to benefit the lung, it is raw
Saliva blood-nourishing, tranquilize the mind and promote the intelligence.It to be taken off for physically weak, cold weak pulse of limb, spleen eating less, the deficiency syndrome of the lung is breathed with cough, injury thirst, Heat Diabetes, gas
Anemia is empty, and prolonged illness void is thin, insomnia of palpitating with fear, impotence palace cold.Ginseng has significant excitation to nervous system, and it is living to improve body
Kinetic force reduces fatigue；There is certain therapeutic effect to different types of neurastheniac, increase patient body weight, disappears
Remove or mitigate the symptoms such as general weakness, headache, insomnia.Ginseng is also improved eyesight and enhances the effect of vision dark adaptation.
Quinoa: originating in South America andes region, is print plus the main conventional food of aborigines, there is 5000-7000
The edible and plantation history of many years, since it is with unique nutritive value.It has brought up print plus nationality, and ancient times, Inca was referred to as
For " grain is female ", quinoa is the base-forming food that Quan Guquan nutrition is rich in protein, and plumule accounts for the 30% of seed, and data shows excellent
The protein content of matter quinoa is up to 16%-22% (beef 20%), and quality is suitable with milk powder and meat, is rich in a variety of amino
Acid, wherein there is necessary to human body all 9 kinds of essential amino acids, ratio is appropriate and is easy to absorb, and is especially enriched in and lacks in plant
Lysine, the mineral matter nutritionals such as calcium, magnesium, phosphorus, potassium, iron, zinc, selenium, manganese, copper content is high, is rich in unsaturated fatty acid, flavonoids, B
The multiple beneficials compound such as family vitamin and E family vitamin, choline, glycine betaine, folic acid, alpha-linolenic acid, beta glucan, diet are fine
Dimension cellulose content is up to 7.1%, and cholesterol 0 is free of seitan, and low fat, (305kcal/100g) low in calories, (GI is glycemic for hypoglycemia
Value 35, glycemic criteria 55), it is nearly all classic in common food.Quinoa has balanced extra-nutrition, enhancing body function
Constitution can, be repaired, immune and endocrine is adjusted, improves the function such as physical stress ability, prevention disease, anticancer, weight-reducing, adjuvant treatment
It is edible to be suitable for all groups for effect.
Jujube: it is sweet in flavor, it is warm-natured.Returns spleen, stomach, the heart channel of Hang-Shaoyin.With tonifying middle-Jiao and Qi, nourishing blood and tranquilization.For spleen eating less, it is out of strength just
It is half congealed, hysteria of womam.Jujube rich in protein, fat, carbohydrate, carrotene, B family vitamin, vitamin C, citrin and
The nutritional ingredients such as calcium, phosphorus, iron and cyclic adenosine monophosphate.Wherein ascorbic content comes out at the top in fruit, have vitamin king it
Laudatory title has the effect of blood-enriching face-nourishing treatment insomnia.There are also supplement calciums, promote sleep, the effect prevented hair loss.
Fructus lycii: it is sweet in flavor, it is mild-natured.Return liver and kidney channel.It is the dual-purpose of drug and food food of Ministry of Public Health's approval with various health care functions
Object has nourishing liver and kidney, benefiting shrewd head.As medicinal, it to be used for consumption consumptive loss, soreness of waist and knee joint, dizziness and tinnitus, impotence and seminal emission is interior
Heat is quenched one's thirst, blood deficiency chlorosis, blurred vision.Proper amount of edible is good for health, has and adjusts immune, enhancing non-specific immune function,
Disease resistance is improved, fatigue is eliminated, adjusts blood pressure and blood lipoid, enhances hematopoiesis function, protects reproductive system.Anti-aging, it is antifatigue, resist
Radiation improves eyesight, beautifying face and moistering lotion, moisturizing.
Cyperue esculentus: cyperue esculentus is also known as brave nut, Chufa, and underground walnut is a kind of high-quality, high yield, comprehensive utilization value
Very high oil, grain are mostly used novel crop.Cyperue esculentus fat content is up to 35% or so, and oil quality is fabulous, because it is rich in insatiable hunger
And fatty acid, there is good antioxidation, therefore olive oil and apricot kernel oil that can be best with current quality compare favourably.Starch contains
Amount up to 30% or so, sugar content is up to 25% or so, and protein content is up to 10% or so -- can wherein be disappeared by pepsin
Change the protein absorbed and account for 70.59%, digestibility is high, and contains 17 kinds of amino acid, wherein arginine, entirely appropriate children and fortune
Moving group, vegetal pole horn of plenty.Prevention myocardial necrosis, myocardial infarction, artery sclerosis, cardiovascular disease, stomach neoplasm are helped rich in magnesium,
The problems such as arthritis, gastric concretion, leukaemia, diabetes, cataract, organ senescence disease, ostealleosis, occurs.Rich in manganese to prevention bone
Matter is loose, and adjusting cell and histologic lesion etc. caused by the generation and release and the various environmental hazards of prevention and treatment of insulin has very
Good effect also has medical value, and " China's book on Chinese herbal medicine outline " is recorded, and cyperue esculentus stem tuber is pungent, Gan Wen, there is soothing the liver promoting the circulation of qi, is good for
Spleen and stomach invigorating effect, especially to stagnation of QI due to depression of the liver abdominal fullness and distention caused by costalgia extremely, uncomfortable in chest, the taste stagnation of the circulation of vital energy, indigestion and loss of appetite, spleen of having a stomachache
Empty deficiency of food, dyspepsia stagnation, indigestion etc. have good improvement.
The present invention also provides a kind of preparation methods of ginseng ternary peptide beverage, comprising:
Step 1: taking ginseng cold soaking 10-14 hours, boils extraction 2 times, and first time 2-3 hour second 1-2 hours, closes
It is filtered after and, filtrate is chromatographed by chromatographic column, and filler is D101 macroreticular resin, and water elution is added to add ethyl alcohol to carry out to colourless
Elution collects eluent, ethyl alcohol is recovered under reduced pressure, and being concentrated into density is 1.05-1.10, and general ginsenoside solution is made；Filter residue adds
Enter the 2-4 times of water measured, fluid is ground into using colloid mill, ginseng pulp is made；
Step 2: 4-6 times of water cooling is added in quinoa and soaks 10-14h, jujube, fructus lycii, cyperue esculentus are separately added into 2-4 times of water
Cold soaking 10-14h, is ground with colloid mill respectively；
Step 3: ground ginseng pulp, quinoa, jujube, fructus lycii and cyperue esculentus are uniformly mixed, and heat 90-100 DEG C,
It is kept for 25-40 minutes, then is cooled to 50-60 DEG C, composite hydrolytic enzyme is added, hydrolyzed 3-8 hours, mixed in 55-60 DEG C of heat preservation
Close liquid；
Step 4: the mixed liquor heating after the completion of step 3 is hydrolyzed keeps the temperature enzyme deactivation, obtains enzyme deactivation mixed liquor；
Step 5: by step 4 enzyme deactivation mixed liquor centrifugal filtration, taking supernatant, and polypeptide and polysaccharide solution is made；
Step 6: step 1 general ginsenoside solution is mixed with the polypeptide of step 5 and polysaccharide solution, by ultrafiltration membrane
Ultrafiltration, temperature are 30-35 DEG C, pressure 25bar, obtain clear solution, sterilize to get ginseng ternary peptide beverage.
Preferably, the ginseng of the step 1 is the 5 years fresh garden ginsents in Changbai Mountain.
Preferably, the concentration of alcohol of the step 1 is 60%.
Preferably, the composite hydrolytic enzyme of the step three includes alpha-amylase, cellulase, neutral papain
With tryptose hydrolase.
Preferably, the composite hydrolytic enzyme, in parts by weight, comprising:
0.1 part of alpha-amylase, 0.1-0.2 parts of cellulase, 0.1-0.2 parts of neutral papain and tryptose hydrolase
0.05-0.1 parts.
Preferably, the additional amount of the composite hydrolytic enzyme is the 3-6% of raw material total amount.
Preferably, the step 4 is warming up to 90-100 DEG C, and the time for keeping the temperature enzyme deactivation is 30-40 minutes.
Beneficial effects of the present invention
The present invention provides a kind of ginseng ternary peptide beverage and preparation method thereof, by be compounded with five kinds of great nutritive values with
The former plant of special efficacy, including ginseng, fructus lycii, jujube, quinoa, cyperue esculentus, according to respective contained nutritional ingredient and effect,
Under suitable extracting factor, human consumption's principle of absorption is simulated, so that effective component is extracted utilization as far as possible, both guaranteed
Food makes full use of, and can increase the demand of health of people health care.
This method uses five kinds of different health foods, by monarch, after mixing in different proportions, with complex enzyme enzyme
Solution extracts the polypeptide for obtaining a variety of sources, and playing food has master to have auxiliary trophic function abundant, and rich in green food
Attribute has extremely strong activity and diversity, and molecular weight is small, the biological function easily absorbed.Effective ingredient in the present invention
For ginsenoside, micromolecule polypeptide, oligomeric maltose, small molecule polysaccharides, Lycium chinense glycopeptide etc..The nutritional components of the product is with small
It is secondly ginsenoside and polysaccharide based on molecular polypeptide.Product of the invention, which has, improves immunity of organisms, adjusts immunity of organism
Function repairs activation and nourishes body growth synthetic cell, strengthen physique, enhances body energy, resist external infringement, while to body
Intracellular metabolite plays the role of bidirectional modulation, reduces and prevent the disease due to caused by internal and external reasons.
Specific embodiment
Present invention firstly provides a kind of ginseng ternary peptide beverages to be prepared from the following raw materials in parts by weight:
5-15 parts of ginseng, 20-50 parts of quinoa, 20-30 parts of jujube (stoning), 20-30 parts of fructus lycii, 20-30 parts of cyperue esculentus.
Preferably, the ginseng ternary peptide beverage is prepared from the following raw materials in parts by weight:
8-10 parts of ginseng, 30-40 parts of quinoa, 22-28 parts of jujube (stoning), 22-28 parts of fructus lycii, 22-28 parts of cyperue esculentus.
The present invention also provides a kind of preparation methods of ginseng ternary peptide beverage, comprising:
Step 1: extraction 2 times is boiled in take ginseng cold soaking 10-14 hours, preferably 12h, first time 2-3 hour, preferably
2h, second 1-2 hours, preferably 1.5h was filtered after merging, and filtrate is chromatographed by chromatographic column, and filler is D101 macropore
Resin adds water elution to add ethyl alcohol to be eluted to colourless, collects eluent, ethyl alcohol is recovered under reduced pressure, and being concentrated into density is 1.05-
1.10, general ginsenoside solution is made；The 2-4 times of water measured is added in filter residue, is ground into fluid using colloid mill, and ginseng pulp is made；
The ginseng is the preferably 5 years fresh garden ginsents in Changbai Mountain, and concentration of alcohol is preferably 60%.
Step 2: 4-6 times of water cooling is added in quinoa and soaks 10-14h, preferably 12h adds jujube, fructus lycii, cyperue esculentus respectively
Enter 2-4 times of water cooling and soak 10-14h, preferably 12h is ground with colloid mill respectively；
Step 3: ground ginseng pulp, quinoa, jujube (stoning), fructus lycii and cyperue esculentus are uniformly mixed, and heat 90-
100 DEG C, preferably 95 DEG C, holding 25-40 minutes, preferably 30 minutes, then it is cooled to 50-60 DEG C, it preferably 55 DEG C, is added multiple
Hydrolase is closed, is hydrolyzed 3-8 hours in 55-60 DEG C of heat preservation, obtains mixed liquor；The composite hydrolytic enzyme, in parts by weight,
It include: 0.1 part of alpha-amylase, 0.1-0.2 parts of cellulase, 0.1-0.2 parts of neutral papain and tryptose hydrolase
0.05-0.1 parts；The additional amount of composite hydrolytic enzyme is the 3-6% of raw material total amount.
Step 4: the mixed liquor after the completion of step 3 is hydrolyzed heats up preferably to 90-100 DEG C, keeps the temperature enzyme deactivation, the guarantor
The time of warm enzyme deactivation is preferably 30-40 minutes, obtains enzyme deactivation mixed liquor；
Step 5: by step 4 enzyme deactivation mixed liquor centrifugal filtration, taking supernatant, and polypeptide and polysaccharide solution is made；
Step 6: step 1 general ginsenoside solution is mixed with the polypeptide of step 5 and polysaccharide solution, by 10000Da
Film ultrafiltration, temperature are 30-35 DEG C, pressure 25bar, obtain clear solution, sterilize to get ginseng ternary peptide beverage；The people
Joining ternary peptide beverage can be liquid, or solid, liquid beverage direct packaging, sterilizing；Solid beverage needs to be added
15-25% dextrin stirring and dissolving 30-40 minutes, is spray-dried, and is dispensed to get ginseng ternary peptide solid beverage.
By the above-mentioned beverage packaging that obtains at being distributed into 30ml/ bottles, daily 30-60ml；Or be distributed into 3g (5g,
8g)/bag, 1-2 bags daily, 60 DEG C or more hot water reconstitute edible.
Effective ingredient is that ginsenoside, micromolecule active polypeptide, oligomeric maltose, small molecule fructus lycii are more in the present invention
Sugar, Lycium chinense glycopeptide etc..
Ginsenoside complicated component, primary efficacy have enhancing immunity of organisms, the effect of fast quick-recovery constitution, rapid recovery
Fatigue improves learning and memory, delays senescence, and has and inhibits nervous centralis, syngignoscism, and analgesia calms the nerves, is antipyretic, promoting serum
Protein synthesis.Have the function of promoting hepatocyte growth and promote DNA synthesis, can be used for treating and preventing hepatitis, liver
Hardening.With anti-inflammatory, removing toxic substances, anti thrombotic action, the proliferation speed of growth of cancer cell can be made to slow down, and have and inhibit cancer cell
Infiltration, promotes apoptosis of tumor cells, inhibits the effects of growth of tumour cell antitumor cell transfer.
The micromolecule active polypeptide is to be referred to as edible immunizing agent using the active peptides of Enzymatic Extraction, can be increased comprehensively
Strong man's body immunity function and immunological regulation are a kind of Modern New immunizing agents, are modern immune treasures.Polypeptide enters human body
Afterwards, rapidly it can be absorbed and utilize, raising natural killer cells (NK) vigor, killing tumor cell, performance broad-spectrum anti-tumor,
Anti-infectious function participates in immunological regulation；It can induce and promote T cell differentiation, maturation, directly stimulate B cell to generate and secrete and exempt from
Epidemic disease globulin (antibody) participates in human immunity；The phagocytic activity for stimulating phagocyte mainly improves it and swallows digestive function, secretion
Function participates in immune response, process of immune regulation；Improve the generation level and expression of receptor water of interleukin 2 (1L-2)
It is flat；Enhance the generation of peripheral mononuclear cells Y- interferon；Enhance SOD activity in serum, effectively prevents sky gas and water, food, ring
The infringement of border pollution.
The Lycium chinense glycopeptide is crude drug in whole fructus lycii " tonifying kidney and benefiting sperm ", has and improves body's immunity, promotes longevity
Effective ingredient, have 5 large effects:
1) adjust immune --- improve NK cell activity, anti-X-ray radiation
2) antitumor --- inhibit tumour growth；There is sensitization to radiotherapy；There is Synergy and attenuation effect to chemotherapy
3) antifatigue, anti-aging --- scavenging activated oxygen and hydroxyl radical free radical
4) liver protection
5) reducing blood lipid, hypoglycemic
The oligomeric maltose, also known as oligosaccharides are difficult or will not be digested, therefore, energy provided by it
Magnitude is very low or does not have at all, can play a role in low-energy food, meets those to the maximum extent and sweet food is liked to worry again
The requirement for the person of getting fat.But there is certain adjusting, improve flora situation in human body intestinal canal, prevent and treat flora imbalance, adjustment and recovery
Enteron aisle normal function treats and prevents constipation, diarrhea, improves the dyslipidemia of hyperlipemia and diabetic, all has good
Good effect.The proliferation for such as promoting Bifidobacterium, reduce toxic tunning and harmful bacteria enzyme generation, inhibit pathogen and
Diarrhea prevents constipation, reduces serum cholesterol etc..
The present invention will be further described in detail combined with specific embodiments below, and the raw material being related in embodiment is
It is commercially available.
Embodiment 1
(1) the 5 years fresh garden ginsents in Changbai Mountain are taken, 150g slice, cold soaking 12 hours, upper foam of floaing boiled extraction 2 times,
It 2 hours for the first time, second 1.5 hours, is filtered after merging, filtrate carries out layer by chromatographic column (filler is D101 macroreticular resin)
Analysis adds water elution to add 60% ethyl alcohol to be eluted to colourless, collects eluent, ethyl alcohol is recovered under reduced pressure, being concentrated into density is
General ginsenoside solution is made in 1.05-1.10 (80 DEG C)；The water of 3 times of amounts is added in filter residue, is ground into fluid using colloid mill, makes
Obtain ginseng pulp；
(2) by quinoa 500g, 5 times of water cooling leaching 12h are added；Jujube (stoning) 300g, fructus lycii 300g, cyperue esculentus 300g, point
Not Jia Ru 3 times of water coolings soak 12h, ground respectively with colloid mill.
(3) by ground ginseng pulp, quinoa, jujube, fructus lycii, cyperue esculentus is uniformly mixed, and adjusting total amount is 6000ml, adds
Heat is kept for 30 minutes to 95 DEG C, then is cooled to 55 DEG C, addition composite hydrolytic enzyme, i.e. alpha-amylase 15.5g, cellulase 31g,
Neutral papain 31g, tryptose hydrolase 15.5g stir at low speed lower 60 DEG C of heat preservations and hydrolyze 6 hours, obtain mixed liquor；
(4) mixed liquor after the completion of hydrolysis is warming up to 95 DEG C, keeps the temperature enzyme deactivation 30 minutes, obtains enzyme deactivation mixed liquor；
(5) by enzyme deactivation mixed liquor centrifugal filtration, supernatant is taken, polypeptide and polysaccharide solution is made；(6) by step (1) people
Ginseng total saposins solution is mixed with step (5) polypeptide and polysaccharide solution, and adjusting total amount is 6000ml, by 10000Da film ultrafiltration, temperature
Degree is 30 DEG C, and pressure 25bar obtains clear solution, according to polypeptide in solution, after total Ginsenosides Content is adjusted, is added
20% dextrin stirring and dissolving 30 minutes, is spray-dried, and is distributed into 3g/ bags to get ginseng ternary peptide beverage.
Embodiment 2
(1) the 5 years fresh garden ginsents in Changbai Mountain are taken, 100g slice, cold soaking 12 hours, upper foam of floaing boiled extraction 2 times,
It 2 hours for the first time, second 1.5 hours, is filtered after merging, filtrate carries out layer by chromatographic column (filler is D101 macroreticular resin)
Analysis adds water elution to add 70% ethyl alcohol to be eluted to colourless, collects eluent, ethyl alcohol is recovered under reduced pressure, being concentrated into density is
General ginsenoside solution is made in 1.05-1.10 (80 DEG C)；The water of 3 times of amounts is added in filter residue, is ground into fluid using colloid mill, makes
Obtain ginseng pulp；
(2) by quinoa 300g, 5 times of water cooling leaching 12h are added；Jujube 200g, fructus lycii 200g, cyperue esculentus 200g are separately added into 3
Times water cooling soaks 12h, is ground respectively with colloid mill.
(3) by ground ginseng pulp, quinoa, jujube, fructus lycii, cyperue esculentus is uniformly mixed, and adjusting total amount is 6000ml, adds
Heat is kept for 30 minutes to 95 DEG C, then is cooled to 55 DEG C, addition composite hydrolytic enzyme, i.e. alpha-amylase 10g, cellulase 15g, in
Property papain 10g, tryptose hydrolase 10 g, stirring at low speed lower 56 DEG C of heat preservations hydrolyzes 4 hours, obtains mixed liquor；
(4) mixed liquor after the completion of hydrolysis is warming up to 95 DEG C, keeps the temperature enzyme deactivation 30 minutes, obtains enzyme deactivation mixed liquor；
(5) by enzyme deactivation mixed liquor centrifugal filtration, supernatant is taken, polypeptide and polysaccharide solution is made；
(6) step (1) general ginsenoside solution is mixed with step (5) polypeptide and polysaccharide solution, adjusting total amount is
6000ml, by 7000Da film ultrafiltration, temperature is 30 DEG C, and pressure 22bar obtains clear solution, according to polypeptide in solution, ginseng
After the content of total saposins is adjusted, 15% dextrin is added, stirring and dissolving 30 minutes, is spray-dried, is distributed into 3g/ bags,
Up to ginseng ternary peptide solid beverage.
Embodiment 3
(1) the 5 years fresh garden ginsents in Changbai Mountain are taken, 50g slice, cold soaking 12 hours, upper foam of floaing boiled extraction 2 times, the
It one time 2 hours, second 1.5 hours, is filtered after merging, filtrate carries out layer by chromatographic column (filler is D101 macroreticular resin)
Analysis adds water elution to add 80% ethyl alcohol to be eluted to colourless, collects eluent, ethyl alcohol is recovered under reduced pressure, being concentrated into density is
General ginsenoside solution is made in 1.05-1.08 (80 DEG C)；The water of 3 times of amounts is added in filter residue, is ground into fluid using colloid mill, makes
Obtain ginseng pulp；
(2) by quinoa 200g, 5 times of water cooling leaching 12h are added；Jujube 200g, fructus lycii 300g, cyperue esculentus 200g are separately added into 3
Times water cooling soaks 12h, is ground respectively with colloid mill.
(3) by ground ginseng pulp, quinoa, jujube, fructus lycii, cyperue esculentus is uniformly mixed, and adjusting total amount is 6000ml, adds
Heat is kept for 30 minutes to 95 DEG C, then is cooled to 55 DEG C, addition composite hydrolytic enzyme, i.e. alpha-amylase 9.5g, cellulase 9.5g,
Neutral papain 9.5g, tryptose hydrolase 5g stir at low speed lower 60 DEG C of heat preservations and hydrolyze 6 hours, obtain mixed liquor；
(4) mixed liquor after the completion of hydrolysis is warming up to 95 DEG C, keeps the temperature enzyme deactivation 30 minutes, obtains enzyme deactivation mixed liquor；
(5) by enzyme deactivation mixed liquor centrifugal filtration, supernatant is taken, polypeptide and polysaccharide solution is made；
(6) step (1) general ginsenoside solution is mixed with step (5) polypeptide and polysaccharide solution, adjusting total amount is
6000ml, by 5000Da film ultrafiltration, temperature is 30 DEG C, and pressure 20bar obtains clear solution, according to polypeptide in solution, ginseng
After the content of Total saponin is adjusted, 15% dextrin is added, stirring and dissolving 30 minutes, is spray-dried, is distributed into 3g/ bags,
Up to ginseng ternary peptide beverage.
One, the detection of product form ingredient.
It is formed for this product, to ginsenoside, polypeptide index is tested and analyzed.
Ginsenoside, by the content assaying method of NY318-1997 ginseng product standard Appendix B ginsenoside
The content of polypeptide is detected by Forint phenol method.
Testing result:
| Index | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Ginsenoside | 3.2mg/g | 2.4mg/g | 1.9mg/g |
| Polypeptide | 198mg/g | 96mg/g | 78mg/g |
By the detection data of table 1 as it can be seen that the beverage that embodiment 1-3 is obtained, the general ginsenoside containing high level and more
Peptide, while the beverage effect that embodiment 1 is prepared is best, illustrates that this method is suitble to be mass produced.
Two, the antifatigue using effect experiment of product
It chooses 80 low fatigable people of 45~60 years old age level constitution and is divided into 4 groups, (men and women each 10 by every group of 20 people
People) it tries out, wherein first group is taken drink prepared by embodiment 1, second group is taken drink prepared by embodiment 2, third
Group takes drink prepared with embodiment 3, and the 4th group is taken the drink only prepared with dextrin, once in the morning and once at night, 7 for control group
Collected application form data preparation acquisition using effect statistical data is shown in Table 2 by day as a treatment course, daily call-on back by phone,
Wherein have physical strength be obviously improved improvement be denoted as effectively, be improved when than not drinking but be not be clearly denoted as it is effective, it is not bright
Being denoted as aobvious improvement is invalid.
Take this product as can be seen from Table 2 after a week, more than 50% people take beverage provided by the present invention resist it is tired
There is apparent improvement in labour face, just has significant improvement to reach 90% or more after taking two weeks；And take the drink of embodiment 1
It significantly improves after product and has reached 100%, the improvement of body is also reached after taking the drink of embodiment 2 and embodiment 3
90% or more.It can be seen that can help to absorption of the human body to small-molecular peptides toward ginseng ternary peptide beverage, to facilitate
Quickly improve function of human body, increase physical strength, develop to good health status, improves human activity, while human body can be improved
Immunity of organisms.
Claims (10)
1. a kind of ginseng ternary peptide beverage, which is characterized in that in parts by weight, be prepared from the following raw materials:
5-15 parts of ginseng, 20-50 parts of quinoa, 20-30 parts of jujube, 20-30 parts of fructus lycii, 20-30 parts of cyperue esculentus.
2. a kind of ginseng ternary peptide beverage according to claim 1, which is characterized in that the ginseng ternary peptide beverage,
In parts by weight, it is prepared from the following raw materials:
8-10 parts of ginseng, 30-40 parts of quinoa, 22-28 parts of jujube, 22-28 parts of fructus lycii, 22-28 parts of cyperue esculentus.
3. a kind of ginseng ternary peptide beverage according to claim 1 or 2, which is characterized in that the ginseng ternary peptide drink
Material is liquid or solid.
4. a kind of preparation method of ginseng ternary peptide beverage according to claim 1 or 2 characterized by comprising
Step 1: taking ginseng cold soaking 10-14 hours, boils and extracts 2 times, first time 2-3 hour, and second 1-2 hours, after merging
Filtering, filtrate chromatographed by chromatographic column, and filler is D101 macroreticular resin, and water elution is added to add ethyl alcohol to be eluted to colourless,
Eluent is collected, ethyl alcohol is recovered under reduced pressure, being concentrated into density is 1.05-1.10, and general ginsenoside solution is made；2-4 is added in filter residue
The water of amount again, is ground into fluid using colloid mill, and ginseng pulp is made；
Step 2: being added 4-6 times of water cooling for quinoa and soak 10-14h, and jujube, fructus lycii, cyperue esculentus are separately added into 2-4 times of water cooling leaching
10-14h is ground with colloid mill respectively；
Step 3: ground ginseng pulp, quinoa, jujube, fructus lycii and cyperue esculentus are uniformly mixed, and heat 90-100 DEG C, are kept
25-40 minutes, then it is cooled to 50-60 DEG C, composite hydrolytic enzyme is added, is hydrolyzed 3-8 hours in 55-60 DEG C of heat preservation, obtains mixed liquor；
Step 4: the mixed liquor heating after the completion of step 3 is hydrolyzed keeps the temperature enzyme deactivation, obtains enzyme deactivation mixed liquor；
Step 5: by step 4 enzyme deactivation mixed liquor centrifugal filtration, taking supernatant, and polypeptide and polysaccharide solution is made；
Step 6: step 1 general ginsenoside solution is mixed with the polypeptide of step 5 and polysaccharide solution, by ultrafiltration membrane ultrafiltration,
Temperature is 30-35 DEG C, pressure 25bar, obtains clear solution, is sterilized to get ginseng ternary peptide beverage.
5. a kind of preparation method of ginseng ternary peptide beverage according to claim 4, which is characterized in that the step 1
Ginseng is the 5 years fresh garden ginsents in Changbai Mountain.
6. a kind of preparation method of ginseng ternary peptide beverage according to claim 4, which is characterized in that the step 1
Concentration of alcohol is 60%.
7. a kind of preparation method of ginseng ternary peptide beverage according to claim 4, which is characterized in that the step three
Composite hydrolytic enzyme include alpha-amylase, cellulase, neutral papain and tryptose hydrolase.
8. a kind of preparation method of ginseng ternary peptide beverage according to claim 4, which is characterized in that the Compound Water
Enzyme is solved, in parts by weight, comprising:
0.1 part of alpha-amylase, 0.1-0.2 parts of cellulase, 0.1-0.2 parts of neutral papain and tryptose hydrolase
0.05-0.1 parts.
9. a kind of preparation method of ginseng ternary peptide beverage according to claim 4, which is characterized in that the Compound Water
The additional amount for solving enzyme is the 3-6% of raw material total amount.
10. a kind of preparation method of ginseng ternary peptide beverage according to claim 4, which is characterized in that the step 4
It is warming up to 90-100 DEG C, the time for keeping the temperature enzyme deactivation is 30-40 minutes.
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