CN109757641A - A kind of ginseng small-molecular peptides beverage and preparation method thereof - Google Patents
A kind of ginseng small-molecular peptides beverage and preparation method thereof Download PDFInfo
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Abstract
The present invention provides a kind of ginseng small-molecular peptides beverage and preparation method thereof, belongs to method for preparing beverage field.The beverage in parts by weight, is prepared from the following raw materials: 5-15 parts of ginseng, 20-30 parts of walnut kernel, 20-30 parts of jujube, 20-30 parts of fructus lycii, 20-50 parts of quinoa, 20-30 parts of cyperue esculentus, 20-30 parts of bone collagen peptide, 15-20 parts of Isin glue collagen peptide.The present invention also provides a kind of preparation methods of ginseng small-molecular peptides beverage.Beverage of the invention is according to respective contained nutritional ingredient and effect, under suitable extracting factor, simulate human consumption's principle of absorption, effective component is set to be extracted utilization as far as possible, both guarantee making full use of for food, and compound Isin glue collagen peptide, bone collagen peptide, increase body to calcareous absorption and utilization, increases the demand of health of people health care.
Description
Technical field
The invention belongs to method for preparing beverage fields, and in particular to a kind of ginseng small-molecular peptides beverage and preparation method thereof.
Background technique
Since the sharp Field doctor of United States Biochemical man U.S. in 1984 has found " active peptides " and active peptides
Effect, opens all kinds of researchs of biological peptide.Nineteen ninety American scientist Luo De mambo scholar formally provides an explanation human diseases, declines
The active peptides of old reason are theoretical, and active peptides are really applied to disease treatment and anti-aging prevention area for the first time.Animal sources
Active peptide is rapidly developed in field of medicaments, is played an important role in the prevention and treatment of disease.Plant active peptides
Also it is widely developed and utilized by people, since vegetable active peptide molecular weight is small, is easier to be absorbed by organisms, such as corn gluten protein peptide,
Peas protein peptide etc., diversified active peptide participate in body vital movement, adjust each organ of body and cells play is each
From with airframe systems function.It repairs, activation, nourish, growing and synthetic cell, the almost all of physiology of adjusting human body are living
Dynamic, nineteen ninety USA New York Times once made " Source of life-mystery active peptides " full page report.Active peptides are controlled for the mankind
It treats and prevents various infectiousness, chronic disease in cell therapy, gene repair therapy, live rapidly by the histocyte for making lesion degenerate
It jumps up to generate the neoblast of health.
Magical effect based on active peptides, makes the purposive acquisition active peptides of body, people in daily health caring diet
Cultivate intensely, the active peptide in food is extracted with modern biology technology, is more effectively absorbed and utilized with more convenient
Form eaten by people, to improve human health constitution.
The prior art is that single plant extracts the nutriments such as small-molecular peptides, is then combined compounding again and solid is made
Or liquid beverage, embody the trophic function of various composition.
Summary of the invention
The object of the present invention is to provide a kind of ginseng small-molecular peptides beverage and preparation method thereof, which is yellowish toner
End has the distinctive fragrance of ginseng, and clean taste, flavour is light palatable with smell, acceptable strong, and has help vigour,
Tranquilize the mind and promote the intelligence reinforces the spleen to benefit the lung, supplement calcium, blood-enriching face-nourishing, improves immunity of organisms, pre- preventing bone rarefaction, antitumor, drop blood
Sugar reduces cholesterol, anti-aging, antifatigue and other effects.
Present invention firstly provides a kind of ginseng small-molecular peptides beverages to be prepared from the following raw materials in parts by weight:
5-15 parts of ginseng, 20-30 parts of walnut kernel, 20-30 parts of jujube, 20-30 parts of fructus lycii, 20-50 parts of quinoa, cyperue esculentus
20-30 parts, 20-30 parts of bone collagen peptide, 15-20 parts of Isin glue collagen peptide.
Preferably, the ginseng small-molecular peptides beverage is prepared from the following raw materials in parts by weight:
8-10 parts of ginseng, 22-28 parts of walnut kernel, 22-28 parts of jujube, 22-28 parts of fructus lycii, 30-40 parts of quinoa, cyperue esculentus
22-28 parts, 22-28 parts of bone collagen peptide, 16-18 parts of Isin glue collagen peptide.
The pharmacological action of each raw material of the present invention are as follows:
Ginseng: slightly warm in nature sweet and slightly bitter taste, returns spleen, lung, the heart, kidney channel.With replenish vital energy, restore pulse, and strengthen and remove vital energy, reinforce the spleen to benefit the lung, it is raw
Saliva blood-nourishing, tranquilize the mind and promote the intelligence.It to be taken off for physically weak, cold weak pulse of limb, spleen eating less, the deficiency syndrome of the lung is breathed with cough, injury thirst, Heat Diabetes, gas
Anemia is empty, and prolonged illness void is thin, insomnia of palpitating with fear, impotence palace cold.Ginseng has significant excitation to nervous system, and it is living to improve body
Kinetic force reduces fatigue；There is certain therapeutic effect to different types of neurastheniac, increase patient body weight, disappears
Remove or mitigate the symptoms such as general weakness, headache, insomnia.Ginseng is also improved eyesight and enhances the effect of vision dark adaptation.
Walnut kernel: it is sweet in flavor, it is warm-natured, return kidney lung large intestine channel.With kidney tonifying, warm lung, ease constipation.Medicine is used for the insufficiency of the kidney yang, waist and knee
Aching and limp, cold of insufficiency type asthma, which is coughed, waits diseases.Walnut kernel nutrient rich in, every hectogram contain 15~20 grams of protein, fatty more, carbon water
10 grams of compound；And contain various trace elements and the minerals and carrotene, riboflavin such as calcium needed by human, phosphorus, iron
Equal multivitamins.It is deep by one of favorite nut based food of common people.Ancient Times in China people early finds that walnut has brain tonic
Nootropic effect.Li Shizhen (1518-1593 A.D.) is said: walnut energy " brain is led in kidney tonifying, beneficial to wisdom ".It is known as " long live ", " Longevity ".Walnut kernel
In protein, amino acid and mineral element rich in, have a very high nutritive value, brain-nourishing intelligence-benefiting, the strong kidney of moistening lung reduces blood
Rouge, prevention coronary heart disease and other effects.
Jujube: it is sweet in flavor, it is warm-natured.Returns spleen, stomach, the heart channel of Hang-Shaoyin.With tonifying middle-Jiao and Qi, nourishing blood and tranquilization.For spleen eating less, it is out of strength just
It is half congealed, hysteria of womam.Jujube rich in protein, fat, carbohydrate, carrotene, B family vitamin, vitamin C, citrin and
The nutritional ingredients such as calcium, phosphorus, iron and cyclic adenosine monophosphate.Wherein ascorbic content comes out at the top in fruit, have vitamin king it
Laudatory title has the effect of blood-enriching face-nourishing treatment insomnia.There are also supplement calciums, promote sleep, the effect prevented hair loss.
Fructus lycii: it is sweet in flavor, it is mild-natured.Return liver and kidney channel.It is the dual-purpose of drug and food food of Ministry of Public Health's approval with various health care functions
Object has nourishing liver and kidney, benefiting shrewd head.As medicinal, it to be used for consumption consumptive loss, soreness of waist and knee joint, dizziness and tinnitus, impotence and seminal emission is interior
Heat is quenched one's thirst, blood deficiency chlorosis, blurred vision.Proper amount of edible is good for health, has and adjusts immune, enhancing non-specific immune function,
Disease resistance is improved, fatigue is eliminated, adjusts blood pressure and blood lipoid, enhances hematopoiesis function, protects reproductive system.Anti-aging, it is antifatigue, resist
Radiation improves eyesight, beautifying face and moistering lotion, moisturizing.
Quinoa: originating in South America andes region, is print plus the main conventional food of aborigines, there is 5000-7000
The edible and plantation history of many years, since it is with unique nutritive value.It has brought up print plus nationality, and ancient times, Inca was referred to as
For " grain is female ", quinoa is the base-forming food that Quan Guquan nutrition is rich in protein, and plumule accounts for the 30% of seed, and data shows excellent
The protein content of matter quinoa is up to 16%-22% (beef 20%), and quality is suitable with milk powder and meat, is rich in a variety of amino
Acid, wherein there is necessary to human body all 9 kinds of essential amino acids, ratio is appropriate and is easy to absorb, and is especially enriched in and lacks in plant
Lysine, the mineral matter nutritionals such as calcium, magnesium, phosphorus, potassium, iron, zinc, selenium, manganese, copper content is high, is rich in unsaturated fatty acid, flavonoids, B
The multiple beneficials compound such as family vitamin and E family vitamin, choline, glycine betaine, folic acid, alpha-linolenic acid, beta glucan, diet are fine
Dimension cellulose content is up to 7.1%, and cholesterol 0 is free of seitan, and low fat, (305kcal/100g) low in calories, (GI is glycemic for hypoglycemia
Value 35, glycemic criteria 55), it is nearly all classic in common food.Quinoa has balanced extra-nutrition, enhancing body function
Constitution can, be repaired, immune and endocrine is adjusted, improves the function such as physical stress ability, prevention disease, anticancer, weight-reducing, adjuvant treatment
It is edible to be suitable for all groups for effect.
Cyperue esculentus: cyperue esculentus is also known as brave nut, Chufa, and underground walnut is a kind of high-quality, high yield, comprehensive utilization value
Very high oil, grain are mostly used novel crop.Cyperue esculentus fat content is up to 35% or so, and oil quality is fabulous, because it is rich in insatiable hunger
And fatty acid, there is good antioxidation, therefore olive oil and apricot kernel oil that can be best with current quality compare favourably.Starch contains
Amount up to 30% or so, sugar content is up to 25% or so, and protein content is up to 10% or so -- can wherein be disappeared by pepsin
Change the protein absorbed and account for 70.59%, digestibility is high, and contains 17 kinds of amino acid, wherein arginine, entirely appropriate children and fortune
Moving group, vegetal pole horn of plenty.Prevention myocardial necrosis, myocardial infarction, artery sclerosis, cardiovascular disease, stomach neoplasm are helped rich in magnesium,
The problems such as arthritis, gastric concretion, leukaemia, diabetes, cataract, organ senescence disease, ostealleosis, occurs.Rich in manganese to prevention bone
Matter is loose, and adjusting cell and histologic lesion etc. caused by the generation and release and the various environmental hazards of prevention and treatment of insulin has very
Good effect also has medical value, and " China's book on Chinese herbal medicine outline " is recorded, and cyperue esculentus stem tuber is pungent, Gan Wen, there is soothing the liver promoting the circulation of qi, is good for
Spleen and stomach invigorating effect, especially to stagnation of QI due to depression of the liver abdominal fullness and distention caused by costalgia extremely, uncomfortable in chest, the taste stagnation of the circulation of vital energy, indigestion and loss of appetite, spleen of having a stomachache
Empty deficiency of food, dyspepsia stagnation, indigestion etc. have good improvement.
Bone collagen peptide can promote the formation of bone, enhance the gelatine original structure under low calcium level, so that it is strong to improve bone
Degree, that is, haved the function that pre- anti-osteoporosis；Protect gastric mucosa and anti-ulcer effect, anti-allergic effects；Beauty delays to decline
Always.
Isin glue collagen peptide has good moisturizing and radiation resistance.Promote microcirculation, increase skin water humidity etc..
Crease-resistant prolong declines, supplement calcium, reinforces calcareous absorption, and osteoporosis occurs in prevention.
The present invention also provides a kind of preparation methods of ginseng small-molecular peptides beverage, comprising:
Step 1: taking ginseng cold soaking 10-14 hours, boils extraction 2 times, and first time 2-3 hour second 1-2 hours, closes
It is filtered after and, it is D101 macroreticular resin that filtrate, which carries out chromatographic stuffing by chromatographic column, and water elution is added to add ethyl alcohol to be washed to colourless
It is de-, eluent is collected, ethyl alcohol is recovered under reduced pressure, being concentrated into density is 1.05-1.10, and general ginsenoside solution is made；Filter residue is added
The 2-4 times of water measured, is ground into fluid using colloid mill, and ginseng pulp is made；
Step 2: 4-6 times of water cooling is added in quinoa and soaks 10-14h, walnut kernel, jujube, fructus lycii, cyperue esculentus are separately added into
2-4 times of water cooling soaks 10-14h, is ground respectively with colloid mill；
Step 3: ground ginseng pulp, quinoa, walnut kernel, jujube, fructus lycii and cyperue esculentus are uniformly mixed, and heat 90-
It 100 DEG C, is kept for 25-40 minutes, then be cooled to 50-60 DEG C, composite hydrolytic enzyme is added, hydrolyzed 3-8 hours in 55-60 DEG C of heat preservation,
Obtain mixed liquor；
Step 4: the mixed liquor heating after the completion of step 3 is hydrolyzed keeps the temperature enzyme deactivation, obtains enzyme deactivation mixed liquor；
Step 5: by step 4 enzyme deactivation mixed liquor centrifugal filtration, taking supernatant, and polypeptide and polysaccharide solution is made；
Step 6: step 1 general ginsenoside solution is mixed with the polypeptide of step 5 and polysaccharide solution, by film ultrafiltration,
Temperature is 30-35 DEG C, pressure 25bar, obtains clear solution, and 25-35% dextrin is added, stirring and dissolving 30-40 minutes, is sprayed
Mist is dry, and Isin glue collagen peptide and bone collagen peptide is then added, and dispenses to get ginseng small-molecular peptides beverage.
Preferably, the ginseng of the step 1 is the 5 years fresh garden ginsents in Changbai Mountain.
Preferably, the concentration of alcohol of the step 1 is 60%.
Preferably, the composite hydrolytic enzyme of the step three includes alpha-amylase, cellulase, neutral papain
With tryptose hydrolase.
Preferably, the composite hydrolytic enzyme, in parts by weight, comprising:
0.1 part of alpha-amylase, 0.1-0.2 parts of cellulase, 0.1-0.2 parts of neutral papain and tryptose hydrolase
0.05-0.1 parts.
Preferably, the additional amount of the composite hydrolytic enzyme is raw material total amount 3-6%
Preferably, the step 4 is warming up to 90-100 DEG C, and the time for keeping the temperature enzyme deactivation is 30-40 minutes.
Preferably, the molecular cut off of ultrafiltration membrane is 1000Da in the step six.
Beneficial effects of the present invention
The present invention provides a kind of ginseng small-molecular peptides beverage and preparation method thereof, by using different source dual-purpose of drug and food
Health food, after mixing in different proportions, to extract with complex enzyme zymohydrolysis method extraction process, the activity for obtaining a variety of sources is more
Peptide, effective ingredient is ginsenoside, small molecule vegetable active polypeptide (ginseng protein peptide, quinoa protein peptides, oily Sha in the present invention
Legumin peptide, walnut protein peptide), oligomeric maltose, small molecule polysaccharides and Lycium chinense glycopeptide, Isin glue collagen peptide, ossein
Protein peptides etc..Playing food has master to have auxiliary trophic function abundant, and rich in ecosystem green food attribute；Two kinds of compounding
Collagen peptide supplement calcium, pre- preventing bone rarefaction, while promoting the microcirculation of body, beauty delays senescence.Production of the invention
Product are with help vigour, kidney tonifying of promoting the production of body fluid, tranquilize the mind and promote the intelligence, supplement calcium, blood-enriching face-nourishing, improve immunity of organisms, and prevention sclerotin is dredged
Pine makes body energy youth state.It is especially suitable for high-intensitive middle aged brain worker, cervical vertebra osteoporosis person.
The former plant of six kinds of great nutritive values and special efficacy, including ginseng, fructus lycii, walnut are compounded in the present invention
Benevolence, jujube (stoning), quinoa, cyperue esculentus, according to respective contained nutritional ingredient and effect, under suitable extracting factor,
Human consumption's principle of absorption is simulated, so that effective component is extracted utilization as far as possible, has both guaranteed making full use of for food, is compounded
Isin glue collagen peptide, bone collagen peptide increase body to calcareous absorption and utilization, increase the demand of health of people health care.
Specific embodiment
Present invention firstly provides a kind of ginseng small-molecular peptides beverages to be prepared from the following raw materials in parts by weight:
5-15 parts of ginseng, 20-30 parts of walnut kernel, 20-30 parts of jujube, 20-30 parts of fructus lycii, 20-50 parts of quinoa, cyperue esculentus
20-30 parts, 20-30 parts of bone collagen peptide, 15-20 parts of Isin glue collagen peptide.
Preferably, the ginseng small-molecular peptides beverage is prepared from the following raw materials in parts by weight:
8-10 parts of ginseng, 22-28 parts of walnut kernel, 22-28 parts of jujube, 22-28 parts of fructus lycii, 30-40 parts of quinoa, cyperue esculentus
22-28 parts, 22-28 parts of bone collagen peptide, 16-18 parts of Isin glue collagen peptide.
The present invention also provides a kind of preparation methods of ginseng small-molecular peptides beverage, comprising:
Step 1: extraction 2 times is boiled in take ginseng cold soaking 10-14 hours, preferably 12h, first time 2-3 hour, preferably
2h, second 1-2 hours, preferably 1.5h was filtered after merging, and it is D101 macropore tree that filtrate, which carries out chromatographic stuffing by chromatographic column,
Rouge adds water elution to add ethyl alcohol to be eluted to colourless, collects eluent, ethyl alcohol is recovered under reduced pressure, and being concentrated into density is 1.05-
1.10, general ginsenoside solution is made；The 2-4 times of water measured is added in filter residue, is ground into fluid using colloid mill, and ginseng pulp is made；
The ginseng is the preferably 5 years fresh garden ginsents in Changbai Mountain, and concentration of alcohol is preferably 60%.
Step 2: being added 4-6 times of water cooling for quinoa and soak 10-14h, preferably 12h, by walnut kernel, jujube, fructus lycii, oily Sha
Beans are separately added into 2-4 times of water cooling and soak 10-14h, and preferably 12h is ground with colloid mill respectively；
Step 3: ground ginseng pulp, quinoa, walnut kernel, jujube (stoning), fructus lycii and cyperue esculentus are uniformly mixed,
90-100 DEG C of heating, preferably 95 DEG C, holding 25-40 minutes, preferably 30 minutes, then it is cooled to 50-60 DEG C, preferably 55
DEG C, composite hydrolytic enzyme is added, is hydrolyzed 3-8 hours in 55-60 DEG C of heat preservation, obtains mixed liquor；The composite hydrolytic enzyme, according to weight
Measure number meter, comprising: 0.1 part of alpha-amylase, 0.1-0.2 parts of cellulase, 0.1-0.2 parts of neutral papain and tryptose
0.05-0.1 parts of hydrolase；The additional amount of composite hydrolytic enzyme is the 3-6% of raw material total amount.
Step 4: the mixed liquor after the completion of step 3 is hydrolyzed heats up preferably to 90-100 DEG C, keeps the temperature enzyme deactivation, the guarantor
The time of warm enzyme deactivation is preferably 30-40 minutes, obtains enzyme deactivation mixed liquor；
Step 5: by step 4 enzyme deactivation mixed liquor centrifugal filtration, taking supernatant, and polypeptide and polysaccharide solution is made；
Step 6: step 1 general ginsenoside solution is mixed with the polypeptide of step 5 and polysaccharide solution, by 1000Da
Film ultrafiltration, temperature are 30-35 DEG C, pressure 25bar, obtain clear solution, and 25-35% dextrin is added, and stirring and dissolving 30-40 divides
Clock is spray-dried, and Isin glue collagen peptide and bone collagen peptide is then added, and is dispensed to get ginseng small-molecular peptides beverage.
By the above-mentioned beverage packaging that obtains at 5g/ bags, 1-2 bags daily, 60 DEG C or more hot water reconstitute use.
Effective ingredient is ginsenoside, small molecule vegetable active polypeptide (ginseng protein peptide, quinoa albumen in the present invention
Peptide, cyperue esculentus protein peptides, walnut protein peptide), oligomeric maltose, small molecule polysaccharides and Lycium chinense glycopeptide, Isin glue collagen peptide,
Bone collagen peptide etc..
The ginsenoside complicated component, primary efficacy have an enhancing immunity of organisms, the effect of fast quick-recovery constitution, fastly
Speed relieves fatigue, improves learning and memory, delays senescence, and has and inhibits nervous centralis, syngignoscism, and analgesia calms the nerves, is antipyretic, promoting
Into serum proteins synthesis.Have the function of promoting hepatocyte growth and promote DNA synthesis, can be used for treating and preventing liver
Scorching, cirrhosis.With anti-inflammatory, removing toxic substances, anti thrombotic action, the proliferation speed of growth of cancer cell can be made to slow down, and have and inhibit
Cancer cell infiltration, promotes apoptosis of tumor cells, inhibits the effects of growth of tumour cell antitumor cell transfer.
The vegetable active polypeptide is referred to as edible immunizing agent using the active peptides of Enzymatic Extraction, can enhance comprehensively
Immune function of human body and immunological regulation are a kind of Modern New immunizing agents, are modern immune treasures.After polypeptide enters human body,
Rapidly it can be absorbed and utilize, improve natural killer cells (NK) vigor, killing tumor cell plays broad-spectrum anti-tumor, resists
Infection effect participates in immunological regulation；It can induce and the differentiation of promotion T cell, maturation, directly stimulation B cell generate simultaneously secretory immune
Globulin (antibody) participates in human immunity；The phagocytic activity for stimulating phagocyte mainly improves it and swallows digestive function, secretion function
Can, participate in immune response, process of immune regulation；Improve the generation level and receptor expression level of interleukin 2 (1L-2)；
Enhance the generation of peripheral mononuclear cells Y- interferon；Enhance SOD activity in serum, effectively prevents sky gas and water, food, environment
The infringement of pollution.
The walnut peptide is a kind of good novel nourishing substance, it not only remains Traditional health care effect of walnut,
It has also carried out new function with science and technology simultaneously and has explored, research shows that wherein glutamic acid rich in, it is to participate in brain
Metabolism unique amino acid, will increase intracerebral acetyl choline content, make Cerebral Cortex Neuronal Cells activity it is vigorous, promote brain tissue
Metabolism restores function of brain cell, for can quickly regain one's vigor through common brain person, protects brain health, enhancing thinking is quick
Prompt degree, effectively prevent failure of memory.(" Walnut extract is to changing by one of " Mountain Western Medicine S University's journal " 2 months 2004 publication
The experimental study of kind mouse learning and memory effect ") glutamic acid and its metabolite in walnut peptide, brain neuroblastoma can be alleviated
Nervous, disturbance state also has significant relaxation effect to the anxiety because of caused by insomnia.Make to fall asleep and be easier, matter of sleeping
Amount is more preferable.So become the new selection of product for invigorating function of brain,
The bone collagen peptide can promote the formation of bone, enhance the gelatine original structure under low calcium level, to improve
Bone strength, that is, haved the function that pre- anti-osteoporosis；Protect gastric mucosa and anti-ulcer effect, anti-allergic effects；Beauty
It delays senescence.
The Isin glue collagen peptide has good moisturizing and radiation resistance.Promote microcirculation, increase skin water profit
Degree etc..Crease-resistant prolong declines, supplement calcium, reinforces calcareous absorption, and osteoporosis occurs in prevention.
The present invention will be further described in detail combined with specific embodiments below, and the raw material being related in embodiment is
It is commercially available.
Embodiment 1
(1) the 5 years fresh garden ginsents in Changbai Mountain are taken, 150g slice, cold soaking 12 hours, upper foam of floaing boiled extraction 2 times,
It 2 hours for the first time, second 1.5 hours, is filtered after merging, filtrate carries out layer by chromatographic column (filler is D101 macroreticular resin)
Analysis adds water elution to add 60% ethyl alcohol to be eluted to colourless, collects eluent, ethyl alcohol is recovered under reduced pressure, being concentrated into density is
General ginsenoside solution is made in 1.05-1.10 (80 DEG C)；The water of 3 times of amounts is added in filter residue, is ground into fluid using colloid mill, makes
Obtain ginseng pulp；
(2) by quinoa 500g, 5 times of water cooling leaching 12h are added；Walnut kernel 300g, jujube 300g, fructus lycii 300g, cyperue esculentus
300g is separately added into 3 times of water cooling leaching 12h, is ground respectively with colloid mill.
(3) by ground ginseng pulp, quinoa, jujube, fructus lycii, cyperue esculentus is uniformly mixed, and adjusting total amount is 6000ml, adds
Heat is kept for 30 minutes to 95 DEG C, then is cooled to 55 DEG C, addition composite hydrolytic enzyme, i.e. alpha-amylase 18.5g, cellulase 37g,
Neutral papain 37g, tryptose hydrolase 18.5g stir at low speed lower 60 DEG C of heat preservations and hydrolyze 6 hours, obtain mixed liquor；
(4) mixed liquor after the completion of hydrolysis is warming up to 95 DEG C, keeps the temperature enzyme deactivation 30 minutes, obtains enzyme deactivation mixed liquor；
(5) by enzyme deactivation mixed liquor centrifugal filtration, supernatant is taken, polypeptide and polysaccharide solution is made；
(6) step (1) general ginsenoside solution is mixed with step (5) polypeptide and polysaccharide solution, adjusting total amount is
6000ml, by 1000Da film ultrafiltration, temperature is 30 DEG C, and pressure 25bar obtains clear solution, according to polypeptide in solution, ginseng
After total saponin content is adjusted, 30% dextrin is added, stirring and dissolving 30 minutes, is spray-dried, obtains solid content about
2000g is added Isin glue collagen peptide 200g, bone collagen peptide 300g, is distributed into 5g/ bags to get ginseng small-molecular peptides beverage.
Embodiment 2
(1) the 5 years fresh garden ginsents in Changbai Mountain are taken, 120g slice, cold soaking 12 hours, upper foam of floaing boiled extraction 2 times,
It 2 hours for the first time, second 1.5 hours, is filtered after merging, filtrate carries out layer by chromatographic column (filler is D101 macroreticular resin)
Analysis adds water elution to add 70% ethyl alcohol to be eluted to colourless, collects eluent, ethyl alcohol is recovered under reduced pressure, being concentrated into density is
General ginsenoside solution is made in 1.05-1.10 (80 DEG C)；The water of 3 times of amounts is added in filter residue, is ground into fluid using colloid mill, makes
Obtain ginseng pulp；
(2) by quinoa 300g, 5 times of water cooling leaching 12h are added；Walnut kernel 200g, jujube 200g, fructus lycii 200g, cyperue esculentus
200g is separately added into 3 times of water cooling leaching 12h, is ground respectively with colloid mill.
(3) by ground ginseng pulp, walnut kernel, quinoa, jujube, fructus lycii, cyperue esculentus is uniformly mixed, adjusts total amount and is
6000ml is heated to 95 DEG C, is kept for 30 minutes, then be cooled to 55 DEG C, and composite hydrolytic enzyme, i.e. alpha-amylase 12.2g, fiber is added
Plain enzyme 18.3g, neutral papain 12.2g, tryptose hydrolase 12.2g stir at low speed lower 56 DEG C of heat preservations and hydrolyze 4 hours,
Obtain mixed liquor；
(4) mixed liquor after the completion of hydrolysis is warming up to 95 DEG C, keeps the temperature enzyme deactivation 30 minutes, obtains enzyme deactivation mixed liquor；
(5) by enzyme deactivation mixed liquor centrifugal filtration, supernatant is taken, polypeptide and polysaccharide solution is made；
(6) step (1) general ginsenoside solution is mixed with step (5) polypeptide and polysaccharide solution, adjusting total amount is
6000ml, by 1000Da film ultrafiltration, temperature is 30 DEG C, and pressure 22bar obtains clear solution, according to polypeptide in solution, ginseng
After the content of total saposins is adjusted, 25% dextrin is added, stirring and dissolving 30 minutes, is spray-dried, obtains solid content about
1800g is added Isin glue collagen peptide 150g, bone collagen peptide 250g, is distributed into 5g/ bags to get ginseng small-molecular peptides beverage.
Embodiment 3
(1) the 5 years fresh garden ginsents in Changbai Mountain are taken, 90g slice, cold soaking 12 hours, upper foam of floaing boiled extraction 2 times, the
It one time 2 hours, second 1.5 hours, is filtered after merging, filtrate carries out layer by chromatographic column (filler is D101 macroreticular resin)
Analysis adds water elution to add 80% ethyl alcohol to be eluted to colourless, collects eluent, ethyl alcohol is recovered under reduced pressure, being concentrated into density is
General ginsenoside solution is made in 1.05-1.08 (80 DEG C)；The water of 3 times of amounts is added in filter residue, is ground into fluid using colloid mill, makes
Obtain ginseng pulp；
(2) by quinoa 200g, 5 times of water cooling leaching 12h are added；Walnut kernel 300g, jujube 200g, fructus lycii 200g, cyperue esculentus
200g is separately added into 3 times of water cooling leaching 12h, is ground respectively with colloid mill.
(3) by ground ginseng pulp, walnut kernel, quinoa, jujube, fructus lycii, cyperue esculentus is uniformly mixed, adjusts total amount and is
6000ml is heated to 95 DEG C, is kept for 30 minutes, then be cooled to 55 DEG C, and composite hydrolytic enzyme, i.e. alpha-amylase 12g, cellulose is added
Enzyme 24g, neutral papain 24g, tryptose hydrolase 6g stir at low speed lower 60 DEG C of heat preservations and hydrolyze 6 hours, mixed
Liquid；
(4) mixed liquor after the completion of hydrolysis is warming up to 95 DEG C, keeps the temperature enzyme deactivation 30 minutes, obtains enzyme deactivation mixed liquor；
(5) by enzyme deactivation mixed liquor centrifugal filtration, supernatant is taken, polypeptide and polysaccharide solution is made；
(6) step (1) general ginsenoside solution is mixed with step (5) polypeptide and polysaccharide solution, adjusting total amount is
6000ml, by 1000Da film ultrafiltration, temperature is 30 DEG C, and pressure 20bar obtains clear solution, according to polypeptide in solution, ginseng
After the content of Total saponin is adjusted, 20% dextrin is added, stirring and dissolving 30 minutes, is spray-dried, obtains solid content about
1500g is added Isin glue collagen peptide 150g, bone collagen peptide 200g, is distributed into 5g/ bags to get ginseng small-molecular peptides beverage.
One, the detection of product form ingredient.
It is formed for this product, to ginsenoside, polypeptide index is tested and analyzed.
Ginsenoside, by the content assaying method of NY318 Appendix B ginsenoside
The content of polypeptide is detected by Forint phenol method.
Testing result:
| Embodiment 1 | Embodiment 2 | Embodiment 3 | |
| Ginsenoside | 3.1mg/g | 2.1mg/g | 1.6mg/g |
| Polypeptide | 326mg/g | 207mg/g | 119mg/g |
By the detection data of table 1 as it can be seen that the beverage that embodiment 1-3 is obtained, the general ginsenoside containing high level and more
Peptide, while the beverage effect that embodiment 1 is prepared is best, illustrates that this method is suitble to be mass produced.
Two, product improves the experiment of memory using effect
The people for choosing 80 poor memories is divided into 4 groups, and every group of 20 people try out, wherein first group is taken embodiment 1
The drink of preparation, second group is taken drink prepared by embodiment 2, and third group takes drink prepared with embodiment 3, and the 4th group is pair
The drink only prepared with dextrin is taken according to group, once in the morning and once at night, observation tracking 15 days, daily call-on back by phone will be collected
Application form data preparation obtain using effect statistical data be shown in Table 2.It is as follows to observe tracking standard: attention is concentrated, memorize speed
Degree improves and memory is lasting, is denoted as effectively；Attention is concentrated, and memorize speed is constant, is remembered feeling relaxed and memory persistently, is denoted as
It is effective；Absent minded, memorize speed is constant and memory is not lasting, and it is invalid to be denoted as.
| In vain | It is effective | Effectively | Obvious effective rate % | |
| Embodiment 1 | 1 | 5 | 14 | 95 |
| Embodiment 2 | 1 | 9 | 10 | 95 |
| Embodiment 3 | 2 | 8 | 10 | 90 |
| Control group | 19 | 1 | 0 | 5 |
After taking this product as can be seen from Table 2 15, beverage provided by the present invention is taken more than 90% people, is being improved
Just there is effective improvement in terms of memory, takes to significantly improve after the drink of embodiment 1 and embodiment 2 and have reached
95%, it can be seen that ginseng small-molecular peptides solid beverage can help to absorption of the human body to small-molecular peptides, to facilitate
Quickly improve function of human body, increase physical strength, quickly regain one's vigor, protects brain health, enhance resourceful degree, effectively improve note
Recall power, develops to good health status.
Claims (10)
1. a kind of ginseng small-molecular peptides beverage, which is characterized in that in parts by weight, be prepared from the following raw materials:
5-15 parts of ginseng, 20-30 parts of walnut kernel, 20-30 parts of jujube, 20-30 parts of fructus lycii, 20-50 parts of quinoa, cyperue esculentus 20-30
Part, 20-30 parts of bone collagen peptide, 15-20 parts of Isin glue collagen peptide.
2. a kind of ginseng small-molecular peptides beverage according to claim 1, which is characterized in that in parts by weight, by with
Lower raw material is made:
8-10 parts of ginseng, 22-28 parts of walnut kernel, 22-28 parts of jujube, 22-28 parts of fructus lycii, 30-40 parts of quinoa, cyperue esculentus 22-28
Part, 22-28 parts of bone collagen peptide, 16-18 parts of Isin glue collagen peptide.
3. a kind of preparation method of ginseng small-molecular peptides beverage according to claim 1 or 2 characterized by comprising
Step 1: taking ginseng cold soaking 10-14 hours, boils and extracts 2 times, first time 2-3 hour, and second 1-2 hours, after merging
Filtering, it is D101 macroreticular resin that filtrate, which carries out chromatographic stuffing by chromatographic column, and water elution is added to add ethyl alcohol to be eluted to colourless,
Eluent is collected, ethyl alcohol is recovered under reduced pressure, being concentrated into density is 1.05-1.10, and general ginsenoside solution is made；2-4 is added in filter residue
The water of amount again, is ground into fluid using colloid mill, and ginseng pulp is made；
Step 2: 4-6 times of water cooling is added in quinoa and soaks 10-14h, walnut kernel, jujube, fructus lycii, cyperue esculentus are separately added into 2-4 times
Water cooling soaks 10-14h, is ground respectively with colloid mill；
Step 3: ground ginseng pulp, quinoa, walnut kernel, jujube, fructus lycii and cyperue esculentus are uniformly mixed, and heat 90-100
DEG C, it is kept for 25-40 minutes, then be cooled to 50-60 DEG C, composite hydrolytic enzyme is added, hydrolyzed 3-8 hours, obtain in 55-60 DEG C of heat preservation
Mixed liquor；
Step 4: the mixed liquor heating after the completion of step 3 is hydrolyzed keeps the temperature enzyme deactivation, obtains enzyme deactivation mixed liquor；
Step 5: by step 4 enzyme deactivation mixed liquor centrifugal filtration, taking supernatant, and polypeptide and polysaccharide solution is made；
Step 6: step 1 general ginsenoside solution is mixed with the polypeptide of step 5 and polysaccharide solution, by film ultrafiltration, temperature
It is 30-35 DEG C, pressure 25bar obtains clear solution, and 25-35% dextrin is added, stirring and dissolving 30-40 minutes, is done by spraying
It is dry, Isin glue collagen peptide and bone collagen peptide is then added, dispenses to get ginseng small-molecular peptides beverage.
4. a kind of preparation method of ginseng small-molecular peptides beverage according to claim 3, which is characterized in that the step 1
Ginseng be the 5 years fresh garden ginsents in Changbai Mountain.
5. a kind of preparation method of ginseng small-molecular peptides beverage according to claim 3, which is characterized in that the step 1
Concentration of alcohol be 60%.
6. a kind of preparation method of ginseng small-molecular peptides beverage according to claim 3, which is characterized in that the step
Three composite hydrolytic enzyme includes alpha-amylase, cellulase, neutral papain and tryptose hydrolase.
7. a kind of preparation method of ginseng small-molecular peptides beverage according to claim 3, which is characterized in that described is compound
Hydrolase, in parts by weight, comprising:
0.1 part of alpha-amylase, 0.1-0.2 parts of cellulase, 0.1-0.2 parts of neutral papain and tryptose hydrolase
0.05-0.1 parts.
8. a kind of preparation method of ginseng small-molecular peptides beverage according to claim 3, which is characterized in that described is compound
The additional amount of hydrolase is raw material total amount 3-6%.
9. a kind of preparation method of ginseng small-molecular peptides beverage according to claim 3, which is characterized in that the step 4
It is warming up to 90-100 DEG C, the time for keeping the temperature enzyme deactivation is 30-40 minutes.
10. a kind of preparation method of ginseng small-molecular peptides beverage according to claim 3, which is characterized in that the step
The molecular cut off of ultrafiltration membrane is 1000Da in rapid six.
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