CN102511854B - Collagen polypeptide drink and its preparation method - Google Patents

Collagen polypeptide drink and its preparation method Download PDF

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Publication number
CN102511854B
CN102511854B CN 201110439598 CN201110439598A CN102511854B CN 102511854 B CN102511854 B CN 102511854B CN 201110439598 CN201110439598 CN 201110439598 CN 201110439598 A CN201110439598 A CN 201110439598A CN 102511854 B CN102511854 B CN 102511854B
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China
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liquid
hide gelatin
sea cucumber
donkey
polypeptide
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CN 201110439598
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Chinese (zh)
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CN102511854A (en
Inventor
孟秀梅
刘昌衡
孙永军
袁文鹏
贾爱荣
张永刚
胡炜
夏雪奎
刘新
张绵松
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山东好当家海洋发展股份有限公司
山东省科学院生物研究所
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Priority to CN 201110439598 priority Critical patent/CN102511854B/en
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Abstract

The invention relates to a collagen polypeptide drink and its preparation method, the method comprises the following steps: taking donkey-hide gelatin, sea cucumber and fish-skin as raw materials, controlling enzymatic hydrolysis condition and respectively carrying out enzymatic hydrolysis on donkey-hide gelatin, sea cucumber and fish-skin collagen, killing enzyme, removing fishy and removing bitter through a membrane, separating and concentrating to obtain the donkey-hide gelatin polypeptide liquid, sea cucumber polypeptide liquid and fish-skin polypeptide liquid with molecular weight of 500Da-3000Da, blending with fruit and vegetable juice, functional sugar, a stabilizing agent and water, homogenizing, filling, sterilizing to prepare the polypeptide drink which possesses the advantages of abundant nutrition, easy absorption and human immunization immunity enhancement. The preparation method has the advantages of advanced process, reasonable technology and strong operationality, and is suitable for large scale production.

Description

A kind of collagen polypeptide drink and preparation method thereof
Technical field
The present invention relates to a kind of non-alcoholic beverage and preparation thereof, especially a kind of collagen polypeptide drink and preparation method thereof.
Background technology
We know that collagen is a kind of extracellular protein, exists with the insoluble fiber form, tool high resistance Zhang Nengli, formation to animal and human's body skin, blood vessel, skeleton, muscles and bones, cartilage is very important, is the important substance of connective tissue, plays the part of the role of conjunctive tissue in zooblast.
Collagen is a macro-molecular protein, and molecular weight is 300KDa, is entwined by three protein chain spirals.Collagen needs to transform process by the protein of complexity after entering human body, just can be absorbed by the body and utilize, and absorption rate only rests in 2.5%.Therefore, solving collagen absorbs difficult one time of problem and becomes scientific circles' focus subject under discussion.
The degraded of collagen appropriateness has just become polypeptide, and the collagen polypeptide molecular weight is little, and the polypeptide chain of its different peptide sections does not have mutual hinge, but is linear structure.Studies show that the absorptivity of collagen polypeptide is between 95%~100%.Simultaneously, collagen polypeptide has multiple body metabolism and physiological regulation function, effects such as promoting immunity, hormone are regulated, antibiotic, antiviral, hypotensive, reducing blood lipid is arranged, and its edible safety is high, is the function factor that current international food circle has development prospect most.
Beverage is meant with water to be base stock, is come out the liquid food that supplies people directly to drink by different prescriptions and fabrication process.Beverage owing to nutritional labelings such as the sugar, acid, breast and each seed amino acid that contain inequality in the beverage of different cultivars, vitamin, inorganic salts, therefore has certain nutrition except that moisture is provided.At present, the soft beverage of China roughly has following a few class: carbonated beverage, fruit-vegetable juice beverage, drinks, teas beverage, milk beverage.Along with the raising of expanding economy, the level of consumption and the enhancing of health care consciousness, people are more and more higher to the requirement of beverage, except the basic function of quenching one's thirst, also need to supplement the nutrients and have health care.And the active peptide beverage not only can carry out corresponding shallow degree adjusting to human body, also can carry out depth adjustment to the human body micro-ecological environment by the absorption of human body to the natural activity peptide.Therefore, the active peptide beverage will be the health beverages of current market in urgent need " natural, healthy, safe, balanced nutritious ".
How to prepare polypeptide class drink and reach the health care purpose by drinking, be the problem that consumer and beverage manufacturing enterprise pay much attention to always.
Summary of the invention
Technical problem to be solved by this invention provides a kind of collagen polypeptide drink and preparation method thereof, the preparation method of this collagen polypeptide drink have the science of selecting materials, technology rationally, the characteristics of strong operability; Adopt the preparation of this method collagen polypeptide drink unique flavor, nutritious, absorptivity is high, and has the health care that strengthens body immunity.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of collagen polypeptide drink, it is characterized in that: it is to be formed by polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, fishskin polypeptide liquid and Juice, function sugar, stabilizing agent, edible organic acid and water allotment, wherein, the percentage by weight of each component is respectively: polypeptides with donkey hide gelatin liquid 10-15%, sea cucumber polypeptide liquid 10-15%, fishskin polypeptide liquid 10-15%, Juice 5-10%, function sugar 6-12%, stabilizing agent 0.05-0.25%, edible organic acid 0.05-0.30, surplus is a water, and the percentage by weight sum of its component is 100%.
Described polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, fishskin polypeptide liquid are to be raw material with donkey-hide gelatin, sea cucumber, fish-skin, respectively donkey-hide gelatin, sea cucumber, collagen are controlled the enzyme enzymolysis, and the enzyme caudacoria that goes out separates and concentrates gained, and its molecular weight is 500Da-3000Da.
Described Juice is that aquatic foods that one or more routines of comprising apple, orange, orange, Huang Tao, tomato, red date are used to prepare the fruits and vegetables of fruit-vegetable juice beverage are squeezed the juice or commercially available concentrated Juice dilutes in proportion and gets.
Described function sugar is one or more the combination in xylo-oligosaccharide, FOS, galactooligosaccharide, oligoisomaltose, sorbierite, maltitol, antierythrite, the xylitol.
Described stabilizing agent is one or more the combination in gelatin, Arabic gum, tamarind seed polysaccharide glue, sesbania gum, agar, sodium alginate, carragheen, pectin, xanthans, cycloheptaamylose, sodium carboxymethylcellulose, hydroxypropul starch, the propylene glycol alginate.
Described edible organic acid is one or more the combination in acetic acid, citric acid, tartaric acid, malic acid, the lactic acid.
A kind of preparation method of collagen polypeptide drink is characterized in that: through following process steps
The choosing and handling of a, major ingredient
Choose donkey-hide gelatin, fresh sea cucumber, fish-skin is primary raw material, and carries out following processing:
A1, slurrying are pulverized the water that the back adds 15~20 times of its weight with the donkey-hide gelatin of choosing, and are heated to 50 ℃~60 ℃, and donkey-hide gelatin is dissolved fully, get the donkey-hide gelatin slurry, and is standby;
The fresh sea cucumber of choosing is removed internal organ, Hui Zui, add the water of 4~8 times of its weight, making beating is heated to 85 ℃~95 ℃, insulation 15~30min, and cooling gets sea cucumber milk, and is standby;
The fresh fish-skin of choosing is added the water of 6~10 times of its weight, and making beating is heated to 50 ℃~60 ℃, insulation 15~20min, treat that fish-skin solution is uniform and stable after, the fish-skin slurry, standby;
It is 3.0~8.0 that a2, enzymolysis are regulated the pH value respectively with donkey-hide gelatin slurry, sea cucumber milk, the fish-skin slurry of a1 step gained, in adding pepsin, trypsase, papain, compound protease, flavor protease, the alkali protease one or more, the control enzymatic hydrolysis condition carries out enzymolysis processing, gets polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, fishskin polypeptide liquid;
Polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, the fishskin polypeptide liquid of gained are heated to 85 ℃~95 ℃ respectively, and the enzyme that goes out is handled 10-15min;
A3, take off raw meat, debitterize and handle 1~5% the yeast liquid that the polypeptides with donkey hide gelatin liquid behind the enzyme that goes out of a2 step gained, sea cucumber polypeptide liquid, fishskin polypeptide liquid is added its weight respectively, under 26 ℃~30 ℃, the condition of 100~200rpm, cultivate 1~3h, take off raw meat and handle;
To take off 2~4% the cycloheptaamylose that polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, fishskin polypeptide liquid after the raw meat adds its weight respectively, under 25 ℃~45 ℃, the condition of 100~150rpm, fully mix 1~1.5h, and carry out debitterize and handle;
A4, membrane separation concentrated polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, the fishskin polypeptide liquid that step a3 is taken off after raw meat, debitterize are handled that contracts filter through 100~120 order filter clothes respectively; Filter cloth adopts the film of 100KDa to carry out degerming, removal of impurities and clarifying treatment respectively after filtering, carry out the 3000Da membrane filtration then respectively, see through liquid and carry out 500Da film desalination and concentration by ultrafiltration respectively, trapped fluid is that 500Da~3000Da is transparent, the polypeptides with donkey hide gelatin liquid of free from extraneous odour, sea cucumber polypeptide liquid, fishskin polypeptide liquid, low temperature is preserved, and is standby;
The preparation of b, Juice
Choose the fruits and vegetables that one or more routines that comprise apple, orange, orange, Huang Tao, tomato, red date are used to prepare fruit-vegetable juice beverage, through cleaning, peeling, stoning are squeezed the juice after handling, and get Juice;
Or choose commercially available concentrated Juice water and dilute, get Juice;
Choosing of c, auxiliary material
Choose in xylo-oligosaccharide, FOS, galactooligosaccharide, oligoisomaltose, sorbierite, maltitol, antierythrite, the xylitol one or more as function sugar, standby;
Choose in gelatin, Arabic gum, tamarind seed polysaccharide glue, sesbania gum, agar, sodium alginate, carragheen, pectin, xanthans, cycloheptaamylose, sodium carboxymethylcellulose, hydroxypropul starch, the propylene glycol alginate one or more as stabilizing agent, standby;
Choose in acetic acid, citric acid, tartaric acid, malic acid, the lactic acid one or more as edible organic acid, standby;
D, allotment and homogeneous
Take by weighing by weight percentage: polypeptides with donkey hide gelatin liquid 10~15%, sea cucumber polypeptide liquid 10~15%, fishskin polypeptide liquid 10~15%, Juice 5~10%, function sugar 6~12%, stabilizing agent 0.05~0.25%, edible organic acid 0.05~0.30%, surplus are water, function sugar, stabilizing agent, edible organic acid water fully being dissolved the back mix with polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, fishskin polypeptide liquid, Juice, is that 50 ℃~60 ℃, pressure are to carry out homogeneous under the condition of 30~50Mpa to handle in temperature;
E, can and sterilization
Solution after the processing of steps d homogeneous is heated to 85 ℃~90 ℃, and insulation 10~20min carries out can while hot, selects the pasteurize mode to carry out sterilization;
Or will carry out sterile filling behind the solution high-temperature instantaneous sterilization after the processing of steps d homogeneous; Promptly get the collagen polypeptide drink.
In the selected raw material of the present invention: donkey-hide gelatin is that the skin of equine species donkey boils and forms, and its flavor is sweet, salty, and property is flat, goes into liver, kidney, lung channel, tonifies the blood and arrest bleeding, nourshing Yin and drynsessmoistening prescription, supports skin and pleases functions such as look.Donkey-hide gelatin is many to be made up of albumen and catabolite, polysaccharose substance and other small-molecule substance, wherein protein content is about 60%~80%, contain 18 seed amino acids (comprising 7 kinds of essential amino acids), 27 kinds of trace elements, wherein essential trace element has 9 kinds of Fe, Cu, Zn, Mn, Cr, Ni, V, Sr etc.In addition, also contain dermatan sulfate (DS) and hyaluronic acid polysaccharides such as (HA) and degraded thereof, binding constituents in the donkey-hide gelatin.
Sea cucumber is Echinodermata (Echinodermata) Holothuroidea (Holothuroidea) animal, is regarded as helping meals delicacies and precious invigorant since ancient times.Modern study shows, contains multiple bioactivators such as sea cucumber polysaccharide, selenka, aliphatic acid and collagen polypeptide in the sea cucumber, has physiological functions such as antitumor, reducing blood lipid, raising immunity.
Fish-skin contains rich in protein and various trace elements, and its protein mainly is the composition of macromolecular collagen and mucopolysaccharide, is Ms's beauty care beauty and health care good merchantable brand.In recent years medical research finds that the leukin-leucine in " fish-skin " has antitumaous effect.
The present invention adopts the modern food biotechnology to carry out enzymolysis processing donkey-hide gelatin, sea cucumber, fish-skin raw material, acquisition has the mixed polypeptide enzymolysis product of many physiological functions such as better digestion and absorption function, antiallergy, enhancing immunity than amino acid, obtain the polypeptide liquid of 500~3000Da by the film concentrating and separating, and the function sugar that is aided with natural fruit and vegetables juice and has unique physiological function, prepare a kind of nutritious, easy absorption, strengthen the polypeptide beverage of body immunity.Through recording: soluble solid is 10~20% in this polypeptide beverage, and polypeptide (in oligopeptide) 3.5~5.5%, polysaccharide are 8~14%.Donkey-hide gelatin of the present invention, sea cucumber, fish-skin enzymolysis prepare debitterize, desalination, the isolation technics advanced person, reliable of the enzymolysis process and the hydrolyzate of polypeptide liquid.The collagen polypeptide drink of preparation presents distinctive color and luster of Juice and local flavor because of adding Juice, when good taste, nutritious food are provided for people, provides abundant collagen polypeptide again, helps strengthening the human immunological competence.Preparation method's technology of this natural donkey-hide gelatin and holothurian fishskin polypeptide drink rationally, make simple, strong operability, be suitable for large-scale production.
The specific embodiment
The present invention will be further described below in conjunction with embodiment.
Embodiment 1
A kind of preparation method of collagen polypeptide drink, its process following process steps:
The choosing and handling of a, major ingredient
Choose donkey-hide gelatin, fresh sea cucumber, fish-skin is primary raw material, and carries out following processing:
A1, slurrying are pulverized the water that the back adds 15 times of its weight with the donkey-hide gelatin of choosing, and are heated to 50 ℃, and donkey-hide gelatin is dissolved fully, get the donkey-hide gelatin slurry, and is standby;
The fresh sea cucumber of choosing is removed internal organ, Hui Zui, add the water of 5 times of its weight, making beating is heated to 85 ℃, insulation 20min, and cooling gets sea cucumber milk, and is standby;
The fresh sole skin of choosing is added the water of 8 times of its weight, and making beating is heated to 55 ℃, insulation 20min, treat that fish-skin solution is uniform and stable after, the fish-skin slurry, standby;
It is 3.0 that a2, enzymolysis are regulated the pH value respectively with donkey-hide gelatin slurry, sea cucumber milk, the fish-skin slurry of a1 step gained, in slurries, add pepsin 2000u by every gram protein respectively and carry out enzymolysis, hydrolysis temperature is controlled to be 37 ℃, enzymolysis 2h, then its pH value is adjusted to 5.0 respectively, adds papain, compound protease, flavor protease 2000u by every gram protein, the control hydrolysis temperature is 50 ℃, enzymolysis 1h gets polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, fishskin polypeptide liquid; Wherein papain, compound protease, flavor protease ratio are 2:1:1, wherein, and the compound protease (Protamex) that the Novozymes Company that compound protease adopts is on the market to be sold produces;
Polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, the fishskin polypeptide liquid of gained are heated to 85 ℃ respectively, and the enzyme that goes out is handled 15min;
A3, take off raw meat, debitterize and handle 3% the yeast liquid that the polypeptides with donkey hide gelatin liquid behind the enzyme that goes out of a2 step gained, sea cucumber polypeptide liquid, fishskin polypeptide liquid is added its weight respectively, under 26 ℃, the condition of 150rpm, cultivate 2h, take off raw meat and handle;
To take off 4% the cycloheptaamylose that polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, fishskin polypeptide liquid after the raw meat adds its weight respectively, under 30 ℃, the condition of 100rpm, fully mix 1.5h, and carry out debitterize and handle;
A4, membrane separation concentrated polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, the fishskin polypeptide liquid that step a3 is taken off after raw meat, debitterize are handled that contracts filter through 100 order filter clothes respectively; Filter cloth adopts the film of 100KDa to carry out degerming, removal of impurities and clarifying treatment respectively after filtering, carry out the 3000Da membrane filtration then respectively, see through liquid and carry out 500Da film desalination and concentration by ultrafiltration respectively, trapped fluid is that 500Da~3000Da is transparent, the polypeptides with donkey hide gelatin liquid of free from extraneous odour, sea cucumber polypeptide liquid, fishskin polypeptide liquid, low temperature is preserved, and is standby;
The preparation of b, Juice
Choose fresh orange, orange, yellow peach and fully clean, squeeze the juice after peeling, the stoning, obtain the bright Juice that presses; Wherein, the ratio of orange, orange, Huang Tao is 1:2:1;
Choosing of c, auxiliary material
Choose FOS, oligoisomaltose, xylitol as function sugar, standby; Wherein, the ratio of FOS, oligoisomaltose, xylitol is 1:1:3;
Choose Arabic gum, agar, sodium alginate, carragheen, xanthans as stabilizing agent, standby; Wherein, the ratio of Arabic gum, agar, sodium alginate, carragheen, xanthans is 1:1:0.05:2:0.05;
Choose citric acid, tartaric acid, malic acid as edible organic acid, standby; Wherein, the ratio of citric acid, tartaric acid, malic acid is 1:0.5:5;
D, allotment and homogeneous
Take by weighing by weight percentage: polypeptides with donkey hide gelatin liquid 10%, sea cucumber polypeptide liquid 15%, fishskin polypeptide liquid 15%, Juice 10%, function sugar 8%, stabilizing agent 0.15%, edible organic acid 0.15%, water 41.7%, function sugar, stabilizing agent, edible organic acid water fully being dissolved the back mix with polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, fishskin polypeptide liquid, Juice, is that 50 ℃, pressure are to carry out homogeneous under the condition of 45Mpa to handle in temperature then;
E, can and sterilization
Solution after the processing of steps d homogeneous is heated to 85 ℃, and insulation 20min carries out can while hot, selects the pasteurize mode to carry out sterilization, promptly gets the collagen polypeptide drink.
The prepared collagen polypeptide drink of present embodiment presents yellow and has strong orange fragrance.After measured: soluble solid is 14% in this polypeptide beverage, and polypeptide (in oligopeptide) 4.2%, polysaccharide are 9.7%.
Embodiment 2
A kind of preparation method of collagen polypeptide drink, its process following process steps:
The choosing and handling of a, major ingredient
Choose donkey-hide gelatin, fresh sea cucumber, fish-skin is primary raw material, and carries out following processing:
A1, slurrying are pulverized the water that the back adds 20 times of its weight with the donkey-hide gelatin of choosing, and are heated to 60 ℃, and donkey-hide gelatin is dissolved fully, get the donkey-hide gelatin slurry, and is standby;
The fresh sea cucumber of choosing is removed internal organ, Hui Zui, add the water of 8 times of its weight, making beating is heated to 95 ℃, insulation 15min, and cooling gets sea cucumber milk, and is standby;
The fresh cod skin of choosing is added the water of 10 times of its weight, and making beating is heated to 60 ℃, insulation 15min, treat that fish-skin solution is uniform and stable after, the fish-skin slurry, standby;
It is 8.0 that a2, enzymolysis are regulated the pH value respectively with donkey-hide gelatin slurry, sea cucumber milk, the fish-skin slurry of a1 step gained, in slurries, add trypsase, alkali protease 2000u by every gram protein respectively, 50 ℃ of control hydrolysis temperatures, enzymolysis time is 2.5h, gets polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, fishskin polypeptide liquid; Wherein, the ratio of trypsase, alkali protease is 1:2;
Polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, the fishskin polypeptide liquid of gained are heated to 95 ℃ respectively, and the enzyme that goes out is handled 10min;
A3, take off raw meat, debitterize and handle 5% the yeast liquid that the polypeptides with donkey hide gelatin liquid behind the enzyme that goes out of a2 step gained, sea cucumber polypeptide liquid, fishskin polypeptide liquid is added its weight respectively, under 28 ℃, the condition of 200rpm, cultivate 1h, take off raw meat and handle;
To take off 2% the cycloheptaamylose that polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, fishskin polypeptide liquid after the raw meat adds its weight respectively, under 45 ℃, the condition of 150rpm, fully mix 1h, and carry out debitterize and handle;
A4, membrane separation concentrated polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, the fishskin polypeptide liquid that step a3 is taken off after raw meat, debitterize are handled that contracts filter through 120 order filter clothes at first respectively; Filter cloth adopts the film of 100KDa to carry out degerming, removal of impurities and clarifying treatment respectively after filtering, carry out the 3000Da membrane filtration then respectively, see through liquid and carry out 500Da film desalination and concentration by ultrafiltration respectively, trapped fluid is that 500Da~3000Da is transparent, the polypeptides with donkey hide gelatin liquid of free from extraneous odour, sea cucumber polypeptide liquid, fishskin polypeptide liquid, low temperature is preserved, and is standby;
The preparation of b, Juice
Choose commercially available concentrated fruits and vegetables cider, grape juice as Juice; Wherein, the ratio of cider, grape juice is 1:2;
Choosing of c, auxiliary material
Choose xylo-oligosaccharide, galactooligosaccharide, sorbierite, antierythrite as function sugar, standby; Wherein, the ratio of xylo-oligosaccharide, galactooligosaccharide, sorbierite, antierythrite is 1:1:2:2;
Choose tamarind seed polysaccharide glue, pectin, xanthans, sodium carboxymethylcellulose as stabilizing agent, standby; Wherein, the ratio of tamarind seed polysaccharide glue, pectin, xanthans, sodium carboxymethylcellulose is: 1:0.05:0.05:1;
Choose acetic acid, tartaric acid, lactic acid as edible organic acid, standby; Wherein, the ratio of acetic acid, tartaric acid, lactic acid is 1:1:4;
D, allotment and homogeneous
Take by weighing by weight percentage: polypeptides with donkey hide gelatin liquid 15%, sea cucumber polypeptide liquid 10%, fishskin polypeptide liquid 12%, Juice 5%, function sugar 12%, stabilizing agent 0.25%, edible organic acid 0.05% and water 45.7%, function sugar, stabilizing agent, edible organic acid water are fully dissolved, will mix with polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, fishskin polypeptide liquid behind the Juice dilute with water, are that 60 ℃, pressure are to carry out homogeneous under the condition of 30Mpa to handle in temperature then;
E, can and sterilization
Solution after the processing of steps d homogeneous is carried out high-temperature instantaneous sterilization, carry out sterile filling then, promptly get the collagen polypeptide drink.
The prepared collagen polypeptide drink of present embodiment presents lilac and has the mixing fragrance of strong apple grape.After measured: soluble solid is 16% in this polypeptide beverage, and polypeptide (in oligopeptide) 3.9%, polysaccharide are 11%.
Embodiment 3
A kind of preparation method of collagen polypeptide drink, its process following process steps:
The choosing and handling of a, major ingredient
Choose donkey-hide gelatin, fresh sea cucumber, fish-skin is primary raw material, and carries out following processing:
A1, slurrying are pulverized the water that the back adds 16 times of its weight with the donkey-hide gelatin of choosing, and are heated to 55 ℃, and donkey-hide gelatin is dissolved fully, get the donkey-hide gelatin slurry, and is standby;
The fresh sea cucumber of choosing is removed internal organ, Hui Zui, add the water of 6 times of its weight, making beating is heated to 90 ℃, insulation 30min, and cooling gets sea cucumber milk, and is standby;
The fresh cod skin of choosing is added the water of 6 times of its weight, and making beating is heated to 50 ℃, insulation 20min, treat that fish-skin solution is uniform and stable after, the fish-skin slurry, standby;
It is 7.5 that a2, enzymolysis are regulated the pH value respectively with donkey-hide gelatin slurry, sea cucumber milk, the fish-skin slurry of a1 step gained, in slurries, add trypsase, alkali protease 2000u by every gram protein respectively, 50 ℃ of control hydrolysis temperatures, enzymolysis time is 3h, gets polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, fishskin polypeptide liquid; Wherein, the ratio of trypsase, alkali protease is 1:2;
Polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, the fishskin polypeptide liquid of gained are heated to 90 ℃ respectively, and the enzyme that goes out is handled 15min;
A3, take off raw meat, debitterize and handle 1.5% the yeast liquid that the polypeptides with donkey hide gelatin liquid behind the enzyme that goes out of a2 step gained, sea cucumber polypeptide liquid, fishskin polypeptide liquid is added its weight respectively, under 30 ℃, the condition of 200rpm, cultivate 3h, take off raw meat and handle;
To take off 3% the cycloheptaamylose that polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, fishskin polypeptide liquid after the raw meat adds its weight respectively, under 25 ℃, the condition of 120rpm, fully mix 1.5h, and carry out debitterize and handle;
A4, membrane separation concentrated polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, the fishskin polypeptide liquid that step a3 is taken off after raw meat, debitterize are handled that contracts filter through 120 order filter clothes at first respectively; Filter cloth adopts the film of 100KDa to carry out degerming, removal of impurities and clarifying treatment respectively after filtering, carry out the 3000Da membrane filtration then respectively, see through liquid and carry out 500Da film desalination and concentration by ultrafiltration respectively, trapped fluid is that 500Da~3000Da is transparent, the polypeptides with donkey hide gelatin liquid of free from extraneous odour, sea cucumber polypeptide liquid, fishskin polypeptide liquid, low temperature is preserved, and is standby;
The preparation of b, Juice
Choose fresh abundant cleaning, squeeze the juice after peeling, the stoning, obtain the bright Juice that presses; Wherein, the ratio of apple, tomato, red date is 2:2:1;
Choosing of c, auxiliary material
Choose FOS, oligoisomaltose, maltitol, sorbierite as function sugar, standby; Wherein, the ratio of FOS, oligoisomaltose, maltitol, sorbierite is 2:2:1:1;
Choose gelatin, sesbania gum, cycloheptaamylose, hydroxypropul starch, propylene glycol alginate as stabilizing agent, standby; Wherein, the ratio of gelatin, sesbania gum, cycloheptaamylose, hydroxypropul starch, propylene glycol alginate is 1:1:0.05:0.05:0.05;
Choose acetic acid, tartaric acid, lactic acid as edible organic acid, standby; Wherein, the ratio of acetic acid, tartaric acid, lactic acid is 1:1:3;
D, allotment and homogeneous
Take by weighing by weight percentage: polypeptides with donkey hide gelatin liquid 12%, sea cucumber polypeptide liquid 14%, fishskin polypeptide liquid 10%, Juice 8%, function sugar 6%, stabilizing agent 0.05%, edible organic acid 0.3% and water 49.65%, function sugar, stabilizing agent, edible organic acid water fully being dissolved the back mix with polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, fishskin polypeptide liquid, Juice, is that 55 ℃, pressure are to carry out homogeneous under the condition of 50Mpa to handle in temperature then;
E, can and sterilization
Solution after the processing of steps d homogeneous is carried out high-temperature instantaneous sterilization, carry out sterile filling then, promptly get the collagen polypeptide drink.
The prepared collagen polypeptide drink of present embodiment presents redness and has strong fruit fragrance.After measured: soluble solid is 12% in this polypeptide beverage, and polypeptide (in oligopeptide) 5%, polysaccharide are 9%.It is good, nutritious that it drinks mouthfeel, and the digestibility height is beneficial to and strengthens the human immunological competence.

Claims (1)

1. the preparation method of a collagen polypeptide drink, it is through following process steps:
The choosing and handling of a, major ingredient
Choose donkey-hide gelatin, fresh sea cucumber, sole skin is primary raw material, and carries out following processing:
A1, slurrying are pulverized the water that the back adds 15 times of its weight with the donkey-hide gelatin of choosing, and are heated to 50 ℃, and donkey-hide gelatin is dissolved fully, get the donkey-hide gelatin slurry, and is standby;
The fresh sea cucumber of choosing is removed internal organ, Hui Zui, add the water of 5 times of its weight, making beating is heated to 85 ℃, insulation 20min, and cooling gets sea cucumber milk, and is standby;
The fresh sole skin of choosing is added the water of 8 times of its weight, and making beating is heated to 55 ℃, insulation 20min, treat that fish-skin solution is uniform and stable after, the fish-skin slurry, standby;
It is 3.0 that a2, enzymolysis are regulated the pH value respectively with donkey-hide gelatin slurry, sea cucumber milk, the fish-skin slurry of a1 step gained, in slurries, add pepsin 2000U by every gram protein respectively and carry out enzymolysis, hydrolysis temperature is controlled to be 37 ℃, enzymolysis 2h, then its pH value is adjusted to 5.0 respectively, adds papain, compound protease, flavor protease 2000U by every gram protein, the control hydrolysis temperature is 50 ℃, enzymolysis 1h gets polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, fishskin polypeptide liquid; Wherein papain, compound protease, flavor protease ratio are 2:1:1, wherein, and the compound protease (Protamex) that the Novozymes Company that compound protease adopts is on the market to be sold produces;
Polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, the fishskin polypeptide liquid of gained are heated to 85 ℃ respectively, and the enzyme that goes out is handled 15min;
A3, take off raw meat, debitterize and handle 3% the yeast liquid that the polypeptides with donkey hide gelatin liquid behind the enzyme that goes out of a2 step gained, sea cucumber polypeptide liquid, fishskin polypeptide liquid is added its weight respectively, under 26 ℃, the condition of 150rpm, cultivate 2h, take off raw meat and handle;
To take off 4% the cycloheptaamylose that polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, fishskin polypeptide liquid after the raw meat adds its weight respectively, under 30 ℃, the condition of 100rpm, fully mix 1.5h, and carry out debitterize and handle;
A4, membrane separation concentrated polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, the fishskin polypeptide liquid that step a3 is taken off after raw meat, debitterize are handled that contracts filter through 100 order filter clothes respectively; Filter cloth adopts the film of 100KDa to carry out degerming, removal of impurities and clarifying treatment respectively after filtering, carry out the 3000Da membrane filtration then respectively, see through liquid and carry out 500Da film desalination and concentration by ultrafiltration respectively, trapped fluid is that 500Da~3000Da is transparent, the polypeptides with donkey hide gelatin liquid of free from extraneous odour, sea cucumber polypeptide liquid, fishskin polypeptide liquid, low temperature is preserved, and is standby;
The preparation of b, Juice
Choose fresh orange, orange, yellow peach and fully clean, squeeze the juice after peeling, the stoning, obtain the bright Juice that presses; Wherein, the ratio of orange, orange, Huang Tao is 1:2:1;
Choosing of c, auxiliary material
Choose FOS, oligoisomaltose, xylitol as function sugar, standby; Wherein, the ratio of FOS, oligoisomaltose, xylitol is 1:1:3;
Choose Arabic gum, agar, sodium alginate, carragheen, xanthans as stabilizing agent, standby; Wherein, the ratio of Arabic gum, agar, sodium alginate, carragheen, xanthans is 1:1:0.05:2:0.05;
Choose citric acid, tartaric acid, malic acid as edible organic acid, standby; Wherein, the ratio of citric acid, tartaric acid, malic acid is 1:0.5:5;
D, allotment and homogeneous
Take by weighing by weight percentage: polypeptides with donkey hide gelatin liquid 10%, sea cucumber polypeptide liquid 15%, fishskin polypeptide liquid 15%, Juice 10%, function sugar 8%, stabilizing agent 0.15%, edible organic acid 0.15%, water 41.7%, function sugar, stabilizing agent, edible organic acid water fully being dissolved the back mix with polypeptides with donkey hide gelatin liquid, sea cucumber polypeptide liquid, fishskin polypeptide liquid, Juice, is that 50 ℃, pressure are to carry out homogeneous under the condition of 45MPa to handle in temperature then;
E, can and sterilization
Solution after the processing of steps d homogeneous is heated to 85 ℃, and insulation 20min carries out can while hot, selects the pasteurize mode to carry out sterilization, promptly gets the collagen polypeptide drink.
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