CN102504936A - Process for preparing barley germ oil and low molecular weight peptide by solid enzymolysis-CO2 (carbon dioxide) supercritical extraction - Google Patents
Process for preparing barley germ oil and low molecular weight peptide by solid enzymolysis-CO2 (carbon dioxide) supercritical extraction Download PDFInfo
- Publication number
- CN102504936A CN102504936A CN2011103238889A CN201110323888A CN102504936A CN 102504936 A CN102504936 A CN 102504936A CN 2011103238889 A CN2011103238889 A CN 2011103238889A CN 201110323888 A CN201110323888 A CN 201110323888A CN 102504936 A CN102504936 A CN 102504936A
- Authority
- CN
- China
- Prior art keywords
- molecular weight
- low molecular
- small
- weight peptide
- raw material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention provides a process for preparing barley germ oil and a low molecular weight peptide by solid enzymolysis-CO2 supercritical extraction, which belongs to the technical field of barley germ processing, and is characterized in that (1) the process flow comprises five process: lipoxygenase deactivating, raw material solid enzymolysis, CO2 supercritical extraction on the germ oil, extraction on the low molecular weight peptide and drying on the low molecular weight peptide; (2) the process conditions are shown as follows: in the lipoxygenase deactivating, original lipoxygenase in the raw material of barley germ powder is deactivated in the manner of heating or microwaves and the like; in the raw material solid enzymolysis, distributing water to the raw material of the barley germ powder to enable the water content of the raw material of the barley germ powder to reach 9-15 percent, and then, neutral protease is added for the solid enzymolysis; in the CO2 supercritical extraction on the germ oil, the extraction pressure is 20-40MPa, and the extraction time is 1-2 hours; in the extraction on the low molecular weight peptide, the dregs of the barley germ powder are extracted for 2-3 times by the water to obtain the liquid containing the low molecular weight peptide; and in the drying on the low molecular weight peptide, the liquid containing the low molecular weight peptide is dried in the manner of spray drying or fluidized bed drying to obtain a low molecular weight peptide product. The prepared barley germ oil and the prepared low molecular weight peptide are widely used for industry of foodstuffs, daily chemicals and the like. The barley germ powder is deeply processed by the process for preparing the barley germ oil and the low molecular weight peptide by the solid enzymolysis-CO2 supercritical extraction, i.e. waste is changed into valuable, thereby, the use value and the market value of the barley germ powder are greatly increased.
Description
One, technical field:
The invention provides a kind of solid enzymatic hydrolysis-CO
2Supercritical extraction system highland barley embryo bud oil and small-molecular peptides technology belong to the Semen avenae nudae plumule processing technique field.
Two, technical background:
Highland barley is to being commonly called as of hull-less barley, and on plant classification, belongs to Gramineae, Hordeum.Highland barley is Qinghai-Tibet topmost cereal crop, has characteristics such as precocity, cold-resistant, barren-resistant, stable yields, wide adaptability.Highland barley is being made outstanding contributions aspect the local masses of farmers and herdsmen's problem of food and clothing of solution, but because its processing technology falls behind, is being far from bringing into play its due vital role in the process of lifting oneself out of poverty.Therefore, development highland barley characteristic processing of farm products and comprehensive utilization technique improve its added value, and be extremely urgent.
Semen avenae nudae plumule is the sub product that the highland barley flour course of processing produces, and high-quality highland barley powder process has only after removing plumule and could realize.Because the Semen avenae nudae plumule mouthfeel is relatively poor, only use at present as feed, there is not further intensive processing.Semen avenae nudae plumule contains the composition that grease, food fiber, minerals and vitamins of high-quality protein, the 10-15% of the 30-40% that has an appointment etc. is of value to HUMAN HEALTH.The highland barley small-molecular peptides is the Semen avenae nudae plumule protein hydrolysate, is to be made up of the small peptide of small molecular weight, have anti-oxidant, remove functions such as radical, detoxifcation, strengthening immunity, in food, medicine industry, use very extensive.Highland barley embryo bud oil is to be a kind of grease that raw material is produced with the Semen avenae nudae plumule, and unsaturated fatty acids accounts for about 80%, wherein, oleic acid 17%, linolic acid 55%, linolenic acid 8% also contains abundant VE and VD, is the functional grease that has much the nutrition and health care effect.
Three, summary of the invention:
The object of the invention is intended to the Semen avenae nudae plumule powder is carried out deep processing, adopts solid enzymatic hydrolysis-CO
2Supercritical extraction process prepares highland barley embryo bud oil and small-molecular peptides, and highland barley embryo bud oil that makes and small-molecular peptides are widely used in industries such as food, daily use chemicals.The present invention carries out deep processing with the Semen avenae nudae plumule powder, turns waste into wealth, and has improved its use value and marketable value greatly, and remarkable economical and social benefit are arranged.
Technical scheme of the present invention is:
Fixing enzymolysis-CO
2Supercritical extraction prepares highland barley embryo bud oil and small-molecular peptides technology, it is characterized in that: (1) its technical process is: fats oxidn enzyme-deactivating, raw material solid enzymatic hydrolysis, CO
2Supercritical extraction germ oil, small-molecular peptides extract and dry five operations of small-molecular peptides are accomplished; (2) its processing condition are: the fats oxidn enzyme-deactivating is to utilize modes such as heating or microwave to make that original lipoxidase loses activity in the raw material Semen avenae nudae plumule powder; If not enzyme-deactivating; Enzyme is easy to be activated in the course of processing, makes highland barley embryo bud oil oxidized fast, reduces oil yield; The deactivation of lipoxidase is a prior art, no longer details here; The raw material solid enzymatic hydrolysis is that the Semen avenae nudae plumule powder raw material water distribution with enzyme-deactivating makes its water cut reach 9-15%; Again with the neutral protease mixing and carry out solid enzymatic hydrolysis; Neutral protease and water distribution Semen avenae nudae plumule powder weight ratio are 1-4: 100, and the solid enzymatic hydrolysis temperature is a room temperature, the solid enzymatic hydrolysis time is 24-120 hour; The effect of solid enzymatic hydrolysis not only can become small-molecular peptides with the Semen avenae nudae plumule proteolysis, also can significantly improve the extraction yield of highland barley embryo bud oil; CO
2The supercritical extraction germ oil is that the Semen avenae nudae plumule powder behind the solid enzymatic hydrolysis is carried out CO
2The process of supercritical extraction highland barley embryo bud oil, extracting pressure are 20-40MPa, and the extraction time is 1-2 hour, and the Semen avenae nudae plumule powder oil yield behind the enzymolysis can reach more than 96%; It is to be the process of solvent extraction small-molecular peptides with the plumule granulated slag water behind the supercritical extraction germ oil that small-molecular peptides extracts, extraction time 0.5-1 hour, extracts 2-3 time, and contain the filter body entering subsequent processing of small-molecular peptides; The small-molecular peptides drying is to adopt the liquid that contains small-molecular peptides modes such as spraying drying or fluidised bed drying to carry out the process that drying obtains the small-molecular peptides product.Highland barley embryo bud oil that makes and small-molecular peptides product can be used as healthcare products or nutrition-fortifying agent is directly edible.
The present invention has the following advantages:
1. the present invention is at CO
2Before the supercritical extraction highland barley embryo bud oil germ protein enzymolysis is become small-molecular peptides, greatly reduce oil and combination of proteins power, and then make the extraction yield of highland barley embryo bud oil bring up to more than 96% CO
2The supercritical extraction time foreshortens to 1-2 hour;
2. the present invention need heat except the small-molecular peptides drying process, and all the other are all at room temperature accomplished, and have so not only saved the energy, have also kept the biological activity of small-molecular peptides effectively;
3. highland barley embryo bud oil of being produced and small-molecular peptides product are pollution-free, have intactly kept their pure natural characteristic, therefore, and this technology environment-friendly and green;
4. the present invention carries out deep processing with the Semen avenae nudae plumule powder, turns waste into wealth, and has significantly improved its use value and marketable value, and better economic and social benefit are arranged.
Four, specific embodiments:
Embodiment 1: the lipoxidase in the fats oxidn enzyme-deactivating adopts the heating means deactivation; The raw material solid enzymatic hydrolysis is that to make its water cut be 9% to the Semen avenae nudae plumule powder raw material water distribution with enzyme-deactivating; Again with the neutral protease mixing and carry out solid enzymatic hydrolysis; Neutral protease and water distribution Semen avenae nudae plumule powder weight ratio are 4: 100, and the solid enzymatic hydrolysis temperature is a room temperature, and the solid enzymatic hydrolysis time is 24 hours; CO
2Extracting pressure is 20MPa in the supercritical extraction germ oil, and the extraction time is 2 hours, and the highland barley embryo bud oil oil yield that obtains can reach more than 96%; It is to be solvent extraction Semen avenae nudae plumule granulated slag 2 times with water that small-molecular peptides extracts, and the liquid that contains small-molecular peptides gets into subsequent processing, and is for use; The small-molecular peptides drying is to adopt the liquid that contains small-molecular peptides the mode of fluidised bed drying to carry out drying to obtain the small-molecular peptides product.
Embodiment 2: the lipoxidase in the fats oxidn enzyme-deactivating adopts the heating means deactivation; The raw material solid enzymatic hydrolysis is that to make its water cut be 15% to the Semen avenae nudae plumule powder raw material water distribution with enzyme-deactivating; Again with the neutral protease mixing and carry out solid enzymatic hydrolysis; Neutral protease and water distribution Semen avenae nudae plumule powder weight ratio are 1: 100, and the solid enzymatic hydrolysis temperature is a room temperature, and the solid enzymatic hydrolysis time is 120 hours; CO
2Supercritical extraction pressure is 40MPa in the supercritical extraction germ oil, and the extraction time is 1 hour, and the highland barley embryo bud oil oil yield that obtains can reach more than 96%; It is to be solvent extraction Semen avenae nudae plumule granulated slag 2 times with water that small-molecular peptides extracts, and the liquid that contains small-molecular peptides gets into subsequent processing, and is for use; The small-molecular peptides drying is to adopt the liquid that contains small-molecular peptides spray-dired mode to carry out drying to obtain the small-molecular peptides product.
Embodiment 3: the lipoxidase in the fats oxidn enzyme-deactivating adopts the heating means deactivation; The raw material solid enzymatic hydrolysis is that the Semen avenae nudae plumule powder raw material water distribution behind the enzyme-deactivating is made its water cut is 12%; Again with the neutral protease mixing and carry out solid enzymatic hydrolysis; Neutral protease and water distribution Semen avenae nudae plumule powder weight ratio are 2.5: 100, and the solid enzymatic hydrolysis temperature is a room temperature, and the solid enzymatic hydrolysis time is 60 hours; CO
2Extracting pressure is 30MPa in the supercritical extraction, and the extraction time is 1 hour, and the highland barley embryo bud oil oil yield that obtains can reach more than 96%; It is to be solvent extraction Semen avenae nudae plumule granulated slag 2 times with water that small-molecular peptides extracts, and the liquid that contains small-molecular peptides gets into subsequent processing, and is for use; The small-molecular peptides drying is to adopt the liquid that contains small-molecular peptides spray-dired mode to carry out drying to obtain the small-molecular peptides product.
Claims (1)
1. solid enzymatic hydrolysis-CO
2Supercritical extraction prepares highland barley embryo bud oil and small-molecular peptides technology, it is characterized in that: (1) its technical process is: fats oxidn enzyme-deactivating, raw material solid enzymatic hydrolysis, CO
2Supercritical extraction germ oil, small-molecular peptides extract and dry five operations of small-molecular peptides are accomplished; (2) its processing condition are: the fats oxidn enzyme-deactivating is to utilize the mode of heating or microwave that original lipoxidase in the raw material Semen avenae nudae plumule powder is lost activity; Make the Semen avenae nudae plumule powder raw material water distribution behind the enzyme-deactivating its water cut reach 9-15% in the raw material solid enzymatic hydrolysis; Add the neutral protease mixing again and carry out solid enzymatic hydrolysis; Neutral protease and water distribution Semen avenae nudae plumule powder weight ratio are 1-4: 100; The solid enzymatic hydrolysis temperature is a room temperature, and the solid enzymatic hydrolysis time is 24-120 hour; CO
2Extracting pressure is 20-40MPa in the supercritical extraction germ oil, and the extraction time is 1-2 hour; Water was solvent extraction Semen avenae nudae plumule granulated slag 2-3 time during small-molecular peptides extracted, and must contain the liquid of small-molecular peptides; Adopt the liquid that contains small-molecular peptides the mode of spraying drying or fluidised bed drying to carry out drying in the small-molecular peptides drying and obtain the small-molecular peptides product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103238889A CN102504936B (en) | 2011-10-24 | 2011-10-24 | Process for preparing barley germ oil and low molecular weight peptide by solid enzymolysis-CO2 (carbon dioxide) supercritical extraction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103238889A CN102504936B (en) | 2011-10-24 | 2011-10-24 | Process for preparing barley germ oil and low molecular weight peptide by solid enzymolysis-CO2 (carbon dioxide) supercritical extraction |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102504936A true CN102504936A (en) | 2012-06-20 |
| CN102504936B CN102504936B (en) | 2013-08-28 |
Family
ID=46217072
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011103238889A Expired - Fee Related CN102504936B (en) | 2011-10-24 | 2011-10-24 | Process for preparing barley germ oil and low molecular weight peptide by solid enzymolysis-CO2 (carbon dioxide) supercritical extraction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102504936B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109418677A (en) * | 2017-08-29 | 2019-03-05 | 北京神飞航天应用技术研究院 | A kind of compound protein solid beverage that can improve spacefarer's eyesight |
| CN112205511A (en) * | 2020-10-09 | 2021-01-12 | 黑龙江省科学院大庆分院 | Preparation method of gamma-aminobutyric acid-rich germinated polypeptide powder |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101019599A (en) * | 2007-03-05 | 2007-08-22 | 重庆大学 | Combined prepn process of wheat plumule protein and its derivative product |
| CN101233920A (en) * | 2007-12-17 | 2008-08-06 | 蒋思萍 | Method for preparing highland barley embryo oil extracted by ultrasonic method |
| CN101248867A (en) * | 2007-12-22 | 2008-08-27 | 兰州奇正健康日用品有限公司 | Highland barley essential oil and preparation of supercritical extract thereof |
| CN101418038A (en) * | 2008-11-14 | 2009-04-29 | 华子昂 | Highland barley peptide and preparation method thereof |
| CN101455241A (en) * | 2008-12-31 | 2009-06-17 | 西藏自治区高原生物研究所 | Highland barley bran oil preparation method from highland barley bran oil using water enzyme method |
| CN101617787A (en) * | 2009-07-27 | 2010-01-06 | 中国科学院西北高原生物研究所 | Process for continuously extracting a plurality of products from highland barley |
-
2011
- 2011-10-24 CN CN2011103238889A patent/CN102504936B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101019599A (en) * | 2007-03-05 | 2007-08-22 | 重庆大学 | Combined prepn process of wheat plumule protein and its derivative product |
| CN101233920A (en) * | 2007-12-17 | 2008-08-06 | 蒋思萍 | Method for preparing highland barley embryo oil extracted by ultrasonic method |
| CN101248867A (en) * | 2007-12-22 | 2008-08-27 | 兰州奇正健康日用品有限公司 | Highland barley essential oil and preparation of supercritical extract thereof |
| CN101418038A (en) * | 2008-11-14 | 2009-04-29 | 华子昂 | Highland barley peptide and preparation method thereof |
| CN101455241A (en) * | 2008-12-31 | 2009-06-17 | 西藏自治区高原生物研究所 | Highland barley bran oil preparation method from highland barley bran oil using water enzyme method |
| CN101617787A (en) * | 2009-07-27 | 2010-01-06 | 中国科学院西北高原生物研究所 | Process for continuously extracting a plurality of products from highland barley |
Non-Patent Citations (1)
| Title |
|---|
| 续艳丽等: "超临界CO2萃取法和索氏提取法提取青稞胚芽油天然生育酚的比较研究", 《西北地区第六届色谱学术报告会甘肃省第十一届色谱年会论文集》, 31 December 2010 (2010-12-31) * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109418677A (en) * | 2017-08-29 | 2019-03-05 | 北京神飞航天应用技术研究院 | A kind of compound protein solid beverage that can improve spacefarer's eyesight |
| CN112205511A (en) * | 2020-10-09 | 2021-01-12 | 黑龙江省科学院大庆分院 | Preparation method of gamma-aminobutyric acid-rich germinated polypeptide powder |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102504936B (en) | 2013-08-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104059958B (en) | A kind of production and processing technology of dried porcine saluble | |
| CN101607218A (en) | Corn steam explosion separates and endosperm multi-component co-production utilizes technology | |
| CN104996715B (en) | The technique that a kind of composite fermentation method prepares wheat germ polypeptide | |
| CN102630804A (en) | Production process of walnut peptide powder | |
| CN103859150A (en) | Production technology of aspergillus oryzae fermented soybean meal | |
| CN103555804B (en) | A kind of preparation method of animals and plants mixed protein peptide | |
| CN107557406A (en) | A kind of method that xylo-oligosaccharide is extracted from corncob | |
| CN104876706A (en) | Lucid ganoderma culture medium | |
| CN103642885A (en) | Method for producing wheat biological active peptide by fermentation | |
| CN102669409B (en) | Method for preparing fermentation promoting peptide of fermented feed from mushroom residue | |
| CN102504936B (en) | Process for preparing barley germ oil and low molecular weight peptide by solid enzymolysis-CO2 (carbon dioxide) supercritical extraction | |
| CN104171632A (en) | Universal pig feed | |
| CN104187055A (en) | Pig feed with sweet potato and preparation method thereof | |
| CN103757081B (en) | A kind of industrialized preparing process of oat protein hydrolysate | |
| CN105520156A (en) | Preparation method of grape seed soluble dietary fiber | |
| CN105028926A (en) | Piglet edema prevention pig feed and preparation method thereof | |
| CN103518943A (en) | Method for extracting proteins from rape seed cakes by utilizing multi-frequency ultrasonic waves | |
| CN104543374A (en) | High-value utilization method for high-nutrition peanut hot-pressed cakes | |
| CN103045345B (en) | Production method for preparing highly unsaturated active rapeseed oil by biological enzyme method | |
| CN103898183A (en) | Method for preparing phytone from peanut meal as raw material through biological fermentation process | |
| CN106689641A (en) | Production technology of walnut peptide powder | |
| CN106889297A (en) | A kind of production technology of soy peptide powder | |
| CN102550935A (en) | Method for extracting rice bran nutrients | |
| CN107739724A (en) | A kind of fruits and vegetables leavening | |
| CN105154507A (en) | Feed grade ocean fish oligopeptide meal and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130828 Termination date: 20191024 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |