CN102500135A - Fast dynamic compression chromatography column - Google Patents
Fast dynamic compression chromatography column Download PDFInfo
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- CN102500135A CN102500135A CN2011103247341A CN201110324734A CN102500135A CN 102500135 A CN102500135 A CN 102500135A CN 2011103247341 A CN2011103247341 A CN 2011103247341A CN 201110324734 A CN201110324734 A CN 201110324734A CN 102500135 A CN102500135 A CN 102500135A
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- dynamic compression
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- chromatographic column
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Abstract
The invention discloses a fast dynamic compression chromatography column comprising a cylindrical column tube with openings on both ends; both ends of the column tube are respectively provided with an upper end cover and a lower end cover which close the column tube; a piston is arranged in the middle of the column tube; a hose passes through the upper end cover and the piston and is communicated to the inner cavity of the column tube which is close to one side of the lower end cover; and the fast dynamic compression chromatography column is characterized in that: a pressurize opening and a pressure relief opening which are penetrated are arranged on the upper end cover; the pressurize opening is connected with liquid rejection equipment through a pipeline; and the pressure relief opening is provided with a safe valve. The fast dynamic compression chromatography column adopts a hydraulic method to drive the piston to move and maintain the pressure. Compared with a motor, the fast dynamic compression chromatography column has the advantages of more stable power, higher uniformity, low cost, long service life, high column efficiency, good symmetry, good repeatability and the like on the premise of maintaining rather good separation effect with an analytical column, and can be applied to a chromatography column with large diameter.
Description
Technical field
The invention belongs to chemical field, be specifically related to a kind of chromatographic column.
Background technology
Along with developing rapidly of industries such as pharmacy, biochemical industry, the preparative liquid chromatography isolation technics obtains development and application more and more widely, has become the important method of separation and purifying complex mixture, is particularly useful for the separation of natural products.For sample is effectively separated, the filling of chromatographic column must be satisfied the requirement of continuity, uniformity, stability and the compactness of bed.There is sizable difficulty in the filling of the major diameter post of the filling of chromatographic column, especially commercial production scale, loads large diameter chromatographic column according to conventional method, and bed is unstable, can reduce separating property.
The dynamic axial compress technique is called for short DAC, is the technology that can in the chromatographic column of various scales, keep the good separation effect, also is the best column packing technique in current preparative chromatography field.The core technology of dynamic axial compression column is to adorn post, keep the post pressure and unload post through moving up and down of piston, and the piston periphery has been equipped with the sealing ring of particular design can allow that the piston easy on and off slides, and the sealing that can keep high is simultaneously again pressed.Applied range as: extracted form natural plant, synthetic drug, protein and polypeptide separate preparation, also be the indispensable means of the modernization of Chinese medicine.At present, motion of the piston in this chromatographic column and pressure are kept and are all adopted motor to realize through screw mandrel basically.Because motor cost is high, and the vibrations that produce during machine operation, cause the less stable of chromatographic column, screw mandrel life-span pressure short, that produce inhomogeneous; This also makes it can not be applied to the occasion of the bigger chromatographic column of diameter.
Summary of the invention
The invention provides a kind of solution of the above problems, a kind of working stability, pressure is even, cost is low quick dynamic compression chromatographic column are provided.
Technical scheme of the present invention is a kind of quick dynamic compression chromatographic column; It comprises the column jecket of cylindrical shape both ends open; Said column jecket two ends are respectively arranged with upper end cover and the bottom end cover with said column jecket sealing; Be provided with piston in the middle of the said column jecket, a flexible pipe passes said upper end cover and piston and is communicated in the inner chamber near the said column jecket of said bottom end cover one side, and it is characterized in that: said upper end cover is provided with the pressurization mouth and the pressure relief opening of all perforations; Said pressurization mouthful pipeline connects liquid filling equipment, and said pressure relief opening is provided with safety valve.
The Pressure gauge of the said column jecket chamber pressure of monitoring preferably, is set on the said upper end cover.
Preferably, a said pressurization mouthful inboard is provided with check valve.
Preferably, the exhaust outlet of perforation is set on the said upper end cover, plug removably has been threaded outside the said exhaust outlet.
Preferably, said piston is provided with the sealing device with the said column jecket bore seal of its both sides.
Preferably, said sealing device comprises and being arranged near the L shaped sealing ring of said upper end cover one side with near the O-ring seals of said bottom end cover one side.
Preferably, said bottom end cover is provided with the solvent outlet that runs through.
Preferably, said upper end cover and bottom end cover all removably are connected on the said column jecket through bolt.
A kind of quick dynamic compression chromatographic column of the present invention adopts the mode driven plunger activity of hydraulic pressure and keeps pressure.Compare motor-driven, it is keeping under the prerequisite of the separating effect suitable with analytical column, and it is more stable, more even also to have power, and advantages such as cost is low, the life-span is long, post effect height, symmetry and good reproducibility can be applied to the big chromatographic column of diameter.
Description of drawings
Fig. 1 is the vertical view of a kind of quick dynamic compression chromatographic column of the present invention;
Fig. 2 be among Fig. 1 A-A to the front view of analysing and observe;
Fig. 3 be among Fig. 1 B-B to the front view of analysing and observe.
The specific embodiment
Following specific embodiments of the invention is described in further detail.
To shown in Figure 3, a kind of quick dynamic compression chromatographic column of the present invention comprises the column jecket 10 of cylindrical shape both ends open like Fig. 1.Column jecket 10 two ends are respectively arranged with upper end cover 12 and bottom end cover 14, upper end cover 12 with bottom end cover 14 all through bolt detachably with column jecket 10 closed at both ends.Being provided with piston 16, one flexible pipes 18 in the middle of the column jecket 10 passes upper end cover 12 and is communicated in column jecket 10 inner chambers near bottom end cover 14 1 sides with piston 16.Upper end cover 12 is provided with pressurization mouth 20 and pressure relief opening 22.Pressurization mouthful 20 pipelines connect liquid filling equipment (not shown), can directly use the employed measuring pump of liquid chromatogram.Pressure relief opening 22 is provided with safety valve 24, and safety valve 24 can be adjusted safe pressure by demand.Pressurization mouthful 20 inboards are provided with check valve 26.The Pressure gauge 28 of monitoring column jecket 10 chamber pressures is set, with instant gaging pressure on the upper end cover 12.The exhaust outlet 30 of perforation is set, exhaust outlet 30 outsides plug 32 that removably has been threaded on the upper end cover 12.The hermetically-sealed construction of piston 16 adds one deck O-ring seals 36 by the L shaped sealing ring 34 of one deck and forms; The liquid of 34 pairs of tops of L shaped sealing ring plays a good sealing effectiveness; Below O-ring seals 36 stops solvent upper reaches, below; Simultaneously the below filler is played a cleaning effect, make below condiment be unlikely to get between the tube wall of L shaped sealing ring 34 and column jecket 10, the influence sealing.Bottom end cover 14 is provided with the solvent outlet 38 that runs through.
Its method of operating is:
1. piston 16 is packed in column jecket 10 inner chambers;
2. upper end cover 12 is installed on the column jecket 10, installs suitable safety valve 24 simultaneously;
The reversing column jecket 10, the filler (not shown) of in column jecket 10, packing into;
4. bottom end cover 14 is installed on the column jecket 10;
5. open the plug 32 of exhaust outlet 30, inject solvent through measuring pump,, add upper plug head 32 up to the gas emptying at pressurization mouth 20;
6. continue to inject solvent, observe Pressure gauge 28 readings, after pressure reaches desired value, close measuring pump;
7. will pressurize and mouthfuls 20 back out with the joint 40 of measuring pump, and be screwed in the colvent inlet then, and in column jecket 10 inner chambers, inject solvent through flexible pipe 18, solvent carries out mask work under the effect of filler.
It is hydraulic pressure that piston of the present invention motion and pressure are kept what lean on; Hydraulic power is more stable, and is more even, makes it have the advantages that cost is low, the life-span long, post is imitated height, symmetry and good reproducibility; It is big to load diameter range, and the maintenance separating effect suitable with analytical column.
Above embodiment is merely the present invention's a kind of embodiment wherein, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to claim of the present invention.Should be pointed out that for the person of ordinary skill of the art under the prerequisite that does not break away from the present invention's design, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with accompanying claims.
Claims (8)
1. quick dynamic compression chromatographic column; It comprises the column jecket of cylindrical shape both ends open; Said column jecket two ends are respectively arranged with upper end cover and the bottom end cover with said column jecket sealing; Be provided with piston in the middle of the said column jecket, a flexible pipe passes said upper end cover and piston is communicated in the said column jecket inner chamber near said bottom end cover one side, it is characterized in that: said upper end cover is provided with pressurization mouth and the pressure relief opening that all connects; Said pressurization mouthful pipeline connects liquid filling equipment, and said pressure relief opening is provided with safety valve.
2. a kind of quick dynamic compression chromatographic column according to claim 1 is characterized in that: the Pressure gauge that the said column jecket chamber pressure of monitoring is set on the said upper end cover.
3. a kind of quick dynamic compression chromatographic column according to claim 1 is characterized in that: a said pressurization mouthful inboard is provided with check valve.
4. a kind of quick dynamic compression chromatographic column according to claim 1 is characterized in that: the exhaust outlet of perforation is set on the said upper end cover, and plug removably has been threaded outside the said exhaust outlet.
5. a kind of quick dynamic compression chromatographic column according to claim 1, it is characterized in that: said piston is provided with the sealing device with the said column jecket bore seal of its both sides.
6. a kind of quick dynamic compression chromatographic column according to claim 5 is characterized in that: said sealing device comprises and being arranged near the L shaped sealing ring of said upper end cover one side with near the O-ring seals of said bottom end cover one side.
7. a kind of quick dynamic compression chromatographic column according to claim 1, it is characterized in that: said bottom end cover is provided with the solvent outlet that runs through.
8. a kind of quick dynamic compression chromatographic column according to claim 1, it is characterized in that: said upper end cover and bottom end cover all removably are connected on the said column jecket through bolt.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110324734.1A CN102500135B (en) | 2011-10-24 | 2011-10-24 | Fast dynamic compression chromatography column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110324734.1A CN102500135B (en) | 2011-10-24 | 2011-10-24 | Fast dynamic compression chromatography column |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102500135A true CN102500135A (en) | 2012-06-20 |
| CN102500135B CN102500135B (en) | 2014-01-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110324734.1A Active CN102500135B (en) | 2011-10-24 | 2011-10-24 | Fast dynamic compression chromatography column |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102989199A (en) * | 2012-12-11 | 2013-03-27 | 聊城万合工业制造有限公司 | Inner storage constant-pressure preparative column device |
| CN103263786A (en) * | 2013-05-24 | 2013-08-28 | 江苏迪沃特仪器设备科技有限公司 | Guard column of industrial-preparation-type chromatographic column |
| CN105004806A (en) * | 2015-07-08 | 2015-10-28 | 浙江省海洋水产研究所 | Telescopic spiral combination type immunoaffinity column |
| CN109358147A (en) * | 2018-12-25 | 2019-02-19 | 成都格莱精密仪器有限公司 | A kind of chromatographic column that can quickly load |
| CN110384951A (en) * | 2019-04-10 | 2019-10-29 | 江苏汉邦科技有限公司 | A kind of novel heat insulation chromatographic column |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0762118A2 (en) * | 1995-09-12 | 1997-03-12 | K.D. PHARMA GmbH | Chromatography column |
| CN2906618Y (en) * | 2006-05-02 | 2007-05-30 | 江苏汉邦科技有限公司 | Dynamic axial compression chromatographic column |
| CN200948371Y (en) * | 2006-07-07 | 2007-09-19 | 陈开明 | Axial pressure adding type preparation and chromatography pole |
| CN202289557U (en) * | 2011-10-24 | 2012-07-04 | 利穗科技(苏州)有限公司 | Rapid and dynamic compression chromatography column |
-
2011
- 2011-10-24 CN CN201110324734.1A patent/CN102500135B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0762118A2 (en) * | 1995-09-12 | 1997-03-12 | K.D. PHARMA GmbH | Chromatography column |
| CN2906618Y (en) * | 2006-05-02 | 2007-05-30 | 江苏汉邦科技有限公司 | Dynamic axial compression chromatographic column |
| CN200948371Y (en) * | 2006-07-07 | 2007-09-19 | 陈开明 | Axial pressure adding type preparation and chromatography pole |
| CN202289557U (en) * | 2011-10-24 | 2012-07-04 | 利穗科技(苏州)有限公司 | Rapid and dynamic compression chromatography column |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102989199A (en) * | 2012-12-11 | 2013-03-27 | 聊城万合工业制造有限公司 | Inner storage constant-pressure preparative column device |
| CN102989199B (en) * | 2012-12-11 | 2014-09-03 | 聊城万合工业制造有限公司 | Inner storage constant-pressure preparative column device |
| CN103263786A (en) * | 2013-05-24 | 2013-08-28 | 江苏迪沃特仪器设备科技有限公司 | Guard column of industrial-preparation-type chromatographic column |
| CN105004806A (en) * | 2015-07-08 | 2015-10-28 | 浙江省海洋水产研究所 | Telescopic spiral combination type immunoaffinity column |
| CN105004806B (en) * | 2015-07-08 | 2017-04-05 | 浙江省海洋水产研究所 | Concertina type spiral combination type immune affinity column |
| CN109358147A (en) * | 2018-12-25 | 2019-02-19 | 成都格莱精密仪器有限公司 | A kind of chromatographic column that can quickly load |
| CN110384951A (en) * | 2019-04-10 | 2019-10-29 | 江苏汉邦科技有限公司 | A kind of novel heat insulation chromatographic column |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102500135B (en) | 2014-01-29 |
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