CN103263786A - Guard column of industrial-preparation-type chromatographic column - Google Patents
Guard column of industrial-preparation-type chromatographic column Download PDFInfo
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- CN103263786A CN103263786A CN2013101956681A CN201310195668A CN103263786A CN 103263786 A CN103263786 A CN 103263786A CN 2013101956681 A CN2013101956681 A CN 2013101956681A CN 201310195668 A CN201310195668 A CN 201310195668A CN 103263786 A CN103263786 A CN 103263786A
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Abstract
The invention discloses a guard column of an industrial-preparation-type chromatographic column. The guard column of the industrial-preparation-type chromatographic column comprises column jackets, a piston and a piston driving device, wherein an upper cover plate and a lower cover plate are respectively arranged at the upper ends and the lower ends of the column jackets; the upper cover plate is provided with a hole; the lower cover plate is provided with a material outlet; the piston is arranged in a column jacket inner cavity; the piston is provided with a fluid dispensing device A; a flow phase inlet of the fluid dispensing device A is communicated with the hole through a pipe; a dispensing opening of the fluid dispensing device A is positioned on the lower end surface of the piston; and a wall of the column jacket, between the piston and the lower cover plate, is provided with a material inlet communicated with the inner cavity. According to the guard column, packing corresponding to the chromatographic column is filled automatically, and the blank of the guard column of the industrial-preparation-type chromatographic column is filled; the filling speed is rapid; the pressure is high; the packing granularity is uniform; a bed layer is stable, and the column effect of the guard column is ensured; and the general problem that the whole system separation effect is lowered because of the guard column at present is solved.
Description
Technical field
The present invention relates to the guard column field, be specifically related to a kind of guard column of industrial preparation type chromatographic column.
Background technology
In high performance liquid chromatograph, guard column is installed between injector and the chromatographic column, be used for capturing in advance and flow mutually and sample solution can firmly adsorb by analyzed post, but the service life of chromatographic column be protected and be prolonged to the material that can not be washed by mobile phase can; But the existence of guard column can influence the post of chromatographic column imitates; after chromatogram industry regulation is installed guard column; theoretical cam curve (N) descends and can not surpass 15%; can protect chromatographic column in order to reach; do not have influence on chromatographic isolation again; the internal diameter of guard column should be identical with the internal diameter of chromatographic column or suitable, and its length generally has only 1-2cm.
Chromatographic column can be divided into for the analytical column of analyzing with for separating of two kinds of the preparative columns of purifying by purposes; The claimed post of chromatographic isolation is loaded and the chromatographic column same size; the filler of brand; the technological requirement that filler is loaded is higher; what sell on the market is the guard column of having loaded filler in advance; but the chromatographic column filler kind is very many; a lot of chromatographic columns do not have corresponding guard column to sell in market; for analytical column, can also substitute with the approximate guard column of filler; but preparative column must guarantee that the filler of filler and chromatographic column is identical; unaffected with the quality of guaranteeing product; industrial preparation type chromatographic column particularly; because of the manufacturing cost height; it is big that filler is loaded difficulty, also do not have the guard column of corresponding industrial preparation type chromatographic column in the market.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of guard column of industrial preparation type chromatographic column; it is many and guard column filler filling difficulty is big because of filling kind to solve existing industrial preparation type chromatographic column; cause not having the guard column of corresponding industrial preparation type chromatographic column, the problem that the service life of industrial preparation type chromatographic column is short.
The present invention is achieved through the following technical solutions:
The guard column of industrial preparation type chromatographic column, comprise column jecket, described column jecket upper/lower terminal is respectively equipped with upper cover plate, lower cover, described upper cover plate is provided with the hole, and described lower cover is provided with discharging opening, also comprises the drive unit of piston and piston, described piston is arranged at the column jecket inner chamber, described piston is provided with the A fluid distributing apparatus, and the mobile phase entrance of described A fluid distributing apparatus is communicated with the hole by pipeline, and the distribution openings of described A fluid distributing apparatus is positioned at the lower surface of piston; The wall of described column jecket between piston and lower cover is provided with the charging aperture of communication with cavity.
The further improvement of the present invention scheme is, drive unit comprises hydraulic pump and fuel tank, described upper cover plate is provided with oil-in and the oil-out of communication with cavity, the oil inlet pipe of described hydraulic pump is communicated with fuel tank, the flowline of described hydraulic pump is communicated with oil-in, described oil-out is communicated with fuel tank, and described oil-in and oil-out are respectively equipped with valve.
The further improvement of the present invention scheme is, the flowline of hydraulic pump is provided with pressure switch, and the normally-closed contact of described pressure switch is connected with the power supply of hydraulic pump.
The further improvement of the present invention scheme is that pipeline is spring shape coil pipe.
The further improvement of the present invention scheme is that upper cover plate is connected with the column jecket two ends with the B flange by the A flange respectively with lower cover.
The further improvement of the present invention scheme is, is provided with limited post between piston and the upper cover plate, and an end of described limited post is vertically connected at piston, and the other end is towards upper cover plate.
The further improvement of the present invention scheme is, lower cover is provided with the B fluid distributing apparatus, and the distribution openings of described B fluid distributing apparatus is located at lower cover towards an end of piston, described B fluid distributing apparatus flow mutually that outlet is communicated in discharging opening.
The invention has the advantages that:
One, can load the various types of fillers corresponding with chromatographic column voluntarily, promote piston with drive unit and compress filler, fill up the blank of the guard column of industrial preparation type chromatographic column;
Two, filling speed is fast, the pressure height, and the filler particles degree is even, and bed is stable, has guaranteed that the post of guard column is imitated, and has solved the present ubiquitous problem that causes the reduction of whole system separating effect because of guard column.
Description of drawings
Fig. 1 is the guard column structural representation of industrial preparation type chromatographic column of the present invention.
Fig. 2 is drive unit syndeton schematic diagram of the present invention.
The specific embodiment
The guard column of industrial preparation type chromatographic column as shown in Figure 1, comprise column jecket 9, on the described column jecket 9, following two ends are connected with upper cover plate 3 by A flange 4 and B flange 11 respectively, lower cover 10, described upper cover plate 3 is provided with hole 5, described lower cover 10 is provided with discharging opening 12, the drive unit that also comprises piston 7 and piston 7, described piston 7 is arranged at column jecket 9 inner chambers, described piston 7 is provided with A fluid distributing apparatus 15, the mobile phase entrance 8 of described A fluid distributing apparatus 15 is communicated with hole 5 by spring shape coil pipe, and the distribution openings of described A fluid distributing apparatus 15 is positioned at the lower surface of piston 7; Be provided with limited post 13 between described piston 7 and the upper cover plate 3, an end of described limited post 13 is vertically connected at piston 7, and the other end is towards upper cover plate 3; The wall of described column jecket 9 between piston 7 and lower cover 10 is provided with the charging aperture 14 of communication with cavity; Described lower cover 10 is provided with B fluid distributing apparatus 16, and the distribution openings of described B fluid distributing apparatus 16 is located at lower cover 10 towards an end of piston 7, and the mobile outlet mutually of described B fluid distributing apparatus 16 is communicated in discharging opening 12.
As shown in Figure 2, drive unit comprises hydraulic pump and fuel tank, described upper cover plate 3 is provided with oil-in 1 and the oil-out 2 of communication with cavity, the oil inlet pipe of described hydraulic pump is communicated with fuel tank, the flowline of described hydraulic pump is communicated with oil-in 1, described oil-out 2 is communicated with fuel tank, and described oil-in 1 and oil-out 2 are respectively equipped with valve.The flowline of hydraulic pump is provided with pressure switch, and the normally-closed contact of described pressure switch is connected with the power supply of hydraulic pump.
The output channel of packing pump is communicated in charging aperture 14, open the valve on the output channel, start the packing pump homogenate filler that homogenate is good and squeeze into column jecket 9 inner chambers, the homogenate filler promotes piston 7 up tops until limited post 13 and arrives upper cover plate 3, close packing pump, close the output channel valve; Can between limited post 13 and upper cover plate 3, install contact-making switch additional, the output channel valve installs valve positioner additional, the normally-closed contact of contact-making switch is connected to power supply and the valve positioner of packing pump, realization increases the security of filler operation to the automatic control of packing pump and output channel valve.
The valve open of oil-out 2, the primer fluid press pump, hydraulic oil is imported column jecket 9 by oil-in 1, overflow from oil-out 2 up to hydraulic oil, suspend hydraulic pump, stifled valve of closing oil-out 2, restart hydraulic pump, it is descending to promote piston 7, and the homogenate filler liquid of column jecket 9 inner chambers is subjected to piston 7 extruding and flows out from discharging opening 12, and the homogenate filler is by further compacting; When the pressure of hydraulic oil reaches the operating pressure that pressure switch is set, hydraulic pump stops, and closes the valve of oil-in 1, keeps the pressure of 7 pairs of homogenate fillers of piston; Oil-out 2 is used for discharging the air of column jecket 9, prevents that air is in the compressed generation blast of column jecket.
Hole 5 is connected with injector, and discharging opening 12 links to each other with chromatographic column.During the chromatographic system operate as normal, flow and to be adsorbed by the guard column of industrial preparation type chromatographic column by the material of mobile institute's wash-out mutually mutually with in the sample, thereby reach the purpose of safeguard industries preparative scale chromatography post.
The present invention can also adopt fluid-operated piston or air rammer or motor as drive unit, with push rod driven plunger 7, is to realize easily to one skilled in the art.
Claims (7)
1. the guard column of industrial preparation type chromatographic column, comprise column jecket (9), on the described column jecket (9), following two ends are respectively equipped with upper cover plate (3), lower cover (10), described upper cover plate (3) is provided with hole (5), described lower cover (10) is provided with discharging opening (12), it is characterized in that: the drive unit that also comprises piston (7) and piston (7), described piston (7) is arranged at column jecket (9) inner chamber, described piston (7) is provided with A fluid distributing apparatus (15), the mobile phase entrance (8) of described A fluid distributing apparatus (15) is communicated with hole (5) by pipeline (6), and the distribution openings of described A fluid distributing apparatus (15) is positioned at the lower surface of piston (7); The wall of described column jecket (9) between piston (7) and lower cover (10) is provided with the charging aperture (14) of communication with cavity.
2. the guard column of industrial preparation type chromatographic column as claimed in claim 1; it is characterized in that: described drive unit comprises hydraulic pump and fuel tank; described upper cover plate (3) is provided with oil-in (1) and the oil-out (2) of communication with cavity; the oil inlet pipe of described hydraulic pump is communicated with fuel tank; the flowline of described hydraulic pump is communicated with oil-in (1); described oil-out (2) is communicated with fuel tank, and described oil-in (1) and oil-out (2) are respectively equipped with valve.
3. the guard column of industrial preparation type chromatographic column as claimed in claim 2, it is characterized in that: the flowline of described hydraulic pump is provided with pressure switch, and the normally-closed contact of described pressure switch is connected with the power supply of hydraulic pump.
4. the guard column of industrial preparation type chromatographic column as claimed in claim 1, it is characterized in that: described pipeline (6) is spring shape coil pipe.
5. the guard column of industrial preparation type chromatographic column as claimed in claim 1, it is characterized in that: described upper cover plate (3) is connected with column jecket (9) two ends with B flange (11) by A flange (4) respectively with lower cover (10).
6. the guard column of industrial preparation type chromatographic column as claimed in claim 1; it is characterized in that: be provided with limited post (13) between described piston (7) and the upper cover plate (3); one end of described limited post (13) is vertically connected at piston (7), and the other end is towards upper cover plate (3).
7. the guard column of industrial preparation type chromatographic column as claimed in claim 1; it is characterized in that: described lower cover (10) is provided with B fluid distributing apparatus (16); the distribution openings of described B fluid distributing apparatus (16) is located at lower cover (10) towards an end of piston (7), and the mobile outlet mutually of described B fluid distributing apparatus (16) is communicated in discharging opening (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101956681A CN103263786A (en) | 2013-05-24 | 2013-05-24 | Guard column of industrial-preparation-type chromatographic column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101956681A CN103263786A (en) | 2013-05-24 | 2013-05-24 | Guard column of industrial-preparation-type chromatographic column |
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| Publication Number | Publication Date |
|---|---|
| CN103263786A true CN103263786A (en) | 2013-08-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2013101956681A Pending CN103263786A (en) | 2013-05-24 | 2013-05-24 | Guard column of industrial-preparation-type chromatographic column |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113444203A (en) * | 2021-08-30 | 2021-09-28 | 深圳市检验检疫科学研究院 | Novel molecularly imprinted polymer, organic mercury detection device applying novel molecularly imprinted polymer and detection method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030183566A1 (en) * | 2002-04-02 | 2003-10-02 | Laub Charles F. | Chromatographic device and method of forming a chromatographic device |
| CN200948371Y (en) * | 2006-07-07 | 2007-09-19 | 陈开明 | Axial pressure adding type preparation and chromatography pole |
| CN102500135A (en) * | 2011-10-24 | 2012-06-20 | 利穗科技(苏州)有限公司 | Fast dynamic compression chromatography column |
| CN102836572A (en) * | 2012-09-17 | 2012-12-26 | 於红军 | Double-cavity dynamic compression column for chromatographic separation |
| CN203227330U (en) * | 2013-05-24 | 2013-10-09 | 江苏迪沃特仪器设备科技有限公司 | Guard column of industrially preparative chromatographic column |
-
2013
- 2013-05-24 CN CN2013101956681A patent/CN103263786A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030183566A1 (en) * | 2002-04-02 | 2003-10-02 | Laub Charles F. | Chromatographic device and method of forming a chromatographic device |
| CN200948371Y (en) * | 2006-07-07 | 2007-09-19 | 陈开明 | Axial pressure adding type preparation and chromatography pole |
| CN102500135A (en) * | 2011-10-24 | 2012-06-20 | 利穗科技(苏州)有限公司 | Fast dynamic compression chromatography column |
| CN102836572A (en) * | 2012-09-17 | 2012-12-26 | 於红军 | Double-cavity dynamic compression column for chromatographic separation |
| CN203227330U (en) * | 2013-05-24 | 2013-10-09 | 江苏迪沃特仪器设备科技有限公司 | Guard column of industrially preparative chromatographic column |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113444203A (en) * | 2021-08-30 | 2021-09-28 | 深圳市检验检疫科学研究院 | Novel molecularly imprinted polymer, organic mercury detection device applying novel molecularly imprinted polymer and detection method |
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Application publication date: 20130828 |