CN102836572A - Double-cavity dynamic compression column for chromatographic separation - Google Patents
Double-cavity dynamic compression column for chromatographic separation Download PDFInfo
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- CN102836572A CN102836572A CN2012103439115A CN201210343911A CN102836572A CN 102836572 A CN102836572 A CN 102836572A CN 2012103439115 A CN2012103439115 A CN 2012103439115A CN 201210343911 A CN201210343911 A CN 201210343911A CN 102836572 A CN102836572 A CN 102836572A
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Abstract
The invention provides a double-cavity dynamic compression column for chromatographic separation. The double-cavity dynamic compression column is characterized by comprising a column body, wherein a column head is sleeved in the column body, a seal is arranged between the column head and the inner wall of the column body; the column head partitions the column body to be an upper column cavity and a lower column cavity; the upper column cavity is provided with a feeding opening, an evacuating opening and an air inlet, wherein the feeding opening is connected with the column head by a pipeline; the lower column cavity is provided with a packing inlet and a liquid outlet, wherein the lower column cavity is full of packing. When the double-cavity dynamic compression column is used in a chromatographic separation process, the column head can dynamically raise and fall along with the shrinkage and expansion of the packing so as to cling to a packing level; and the column body and the column head can be manufactured to be great in diameter with acceptable cost.
Description
Technical field
The invention belongs to a kind of chromatography separate apparatus and use device, relate in particular to a kind of compression post that is used for the chromatography analytical instrument.
Background technology
Chromatography separates the separation that is widely used in monomers such as biology, chemical industry, medicine, food additives.
Chromatographic column with filler along with the progress of science and technology is being brought in constant renewal in, to the sexual valence of chromatographic column when practicality the requirement upgraded is constantly proposed.
Volume also constantly changes some filler owing to the variation of flowing phase when chromatography; Therefore need the dynamic compression post; Make the Volume Changes that column cap can corresponding filler and change and keeps being close to of filler face, and present large-scale dynamic compression post adopts hydraulic system, price is costliness especially.
Summary of the invention
The present invention provides a kind of chromatography to separate two-chamber dynamic compression post, and its purpose solves the shortcoming that prior art exists, and its column cap can be kept along with the Volume Changes of filler and being close to of filler, and low cost of manufacture.
The technical solution adopted for the present invention to solve the technical problems is:
Chromatography separates two-chamber dynamic compression post, and it is characterized in that: have cylinder, column cap is set in the cylinder, is provided with sealing between column cap and the inboard wall of cylinder; Column cap is divided into upper prop chamber and lower prop chamber with cylinder, and the upper prop chamber is provided with charging aperture and vacuum orifice and air inlet, and charging aperture is connected with pipeline with column cap, and the lower prop chamber is provided with into feeding port and liquid outlet, and lower prop is full of filler in the chamber.
The sealing that is provided with between column cap and the inboard wall of cylinder comprises that the top is against the sealing ring that the elastomeric material on the inboard wall of cylinder is processed; Establish annular recess on the sealing ring; The annular seal compensating part that ccontaining elastomeric material is processed in the groove is pressed on the sealing compensation spare and is provided with the sealing ring counterpart, connects screw and passes the sealing ring counterpart; The lower end snail that connects screw is located on the column cap; Be arranged with the pressure compensation spring on the connection screw, the top, upper end of pressure compensation spring is against on the screw terminal that connects screw, and top, the lower end of pressure compensation spring is against on the sealing ring counterpart.
A counter-force device connects firmly above cylinder; A column cap lock nut spiral shell is located in the logical screw that is provided with on the counter-force device; The upper end of column cap lock nut is provided with handwheel; The lower end of column cap lock nut is fixedly connected with the column cap guide rod, and the lower end cooperation of column cap guide rod is plugged in the set guide hole of column cap.
Usefulness of the present invention is:
The present invention is in the chromatography process, and column cap can be along with the contraction of filler and expansion and dynamically gone up and down and be close to the filler face, can accept but cylinder and column cap can manufacture very big diameter cost.
Description of drawings
Below in conjunction with accompanying drawing and embodiment the present invention is further specified.
Fig. 1 is a sketch map of the present invention.
The specific embodiment
As shown in Figure 1; The present invention has columniform cylinder 1, and column cap 2 is set in the cylinder 1, and column cap 2 is divided into upper prop chamber 11 and lower prop chamber 12 with the inner chamber of cylinder 1; Upper prop chamber 11 is provided with charging aperture 13 and vacuum orifice 14 and air inlet 18; Charging aperture 13 is connected (connecting with the prior art structure) with column cap 2 with flexible duct 15, and lower prop chamber 12 is provided with into feeding port 16 and liquid outlet 17, is full of filler 3 in the lower prop chamber 12.
Be provided with sealing between the inwall of column cap 2 and cylinder 1; Said sealing comprises pushes up the sealing ring 41 that the elastomeric material on the inwall that is against cylinder 1 is processed; Establish annular recess on the sealing ring 41, be equipped with the annular seal compensating part 42 that elastomeric material is processed in the groove, press on the sealing compensation spare 42 to be provided with sealing ring counterpart 43; Sealing ring counterpart 43 is discoid; The periphery of sealing ring counterpart 43 extends the fastening limit 431 of a circle downwards, and shrink downwards gradually on fastening limit 431, and its end is pressed on the sealing ring counterpart 43.Connect screw 44 and pass the set through hole of sealing ring counterpart 43; The lower end snail that connects screw 44 is located in the screw set on the column cap 2; Connect on the screw 44 and be arranged with pressure compensation spring 45; The top, upper end of pressure compensation spring 45 is against on the screw terminal that connects screw 44, and top, the lower end of pressure compensation spring 45 is against on the sealing ring counterpart 43.
Connecting screw 44 is connected with column cap 2; The elastic force of pressure compensation spring 45 is pressed on the sealing compensation spare 42 it to pressing down sealing ring counterpart 43; Sealing compensation spare 42 is pressed and to the both sides distortion, make sealing ring 41 expand outwardly and closely the top be against on the inwall of cylinder 1, play sealing function.
When column cap 2 moves up and down in cylinder 1 when causing sealing ring 41 wearing and tearing, the elastic force of pressure compensation spring 45 can make sealing compensation spare 42 continue distortion, causes the further distortion of sealing ring 41 and reaches the effect of compensation.
A counter-force device 5 connects firmly above cylinder 1; Counter-force device 5 is one and is fixed on a frame structure of putting on the cylinder 1 with the bolt and nut mode by fixed disk 51,52, pull bar 53; Column cap lock nut 6 spiral shells are located in the logical screw 50 that is provided with on the counter-force device 5; The upper end of column cap lock nut 6 is provided with handwheel 61, and the lower end of column cap lock nut 6 is fixedly connected with column cap guide rod 62, and the lower end of column cap guide rod 62 cooperates and is plugged in the set guide hole 20 of column cap 2.Column cap guide rod 62 cooperates the stability that guarantees column cap 2 with guide hole 20.With handwheel 61 drive stud guide rods 62 rather than adopt hydraulic means can avoid oil pollution that hydraulic means brings and possible equipment fault.
During actual the use, vacuumize through vacuum orifice 14, upper prop chamber 11 air pressure descend, and make column cap 2 up, have enlarged lower prop chamber 12, pack filler 3 into to lower prop chamber 12 through advancing feeding port 16.Import Compressed Gas to upper prop chamber 11 by air inlet 18 then, upper prop chamber 11 pressure rises make column cap 2 descending, and the fillers 3 in the lower prop chamber 12 are compressed.Then from charging aperture 13 input liquid; Liquid gets into column cap 2 continued by flexible duct 15 and gets into filler 3; Filler 3 is along with volume expands or shrinks with contacting of flowing phase, and column cap 2 rises or descends along with the variation of filler 3 volumes and keeps being close to the filler face.
Principle of the present invention is: utilize the Compressed Gas relative pressure after enlarging certain volume in the upper prop chamber 11 to reduce little (when filler 3 shrinks; Column cap 2 is descending; Upper prop chamber 11 will enlarge) and filler is pressurizeed, and compare column cap 2 enlarged areas with gas generation pressure and existing hydraulic system generation pressure; Cost increases and is few, can process the very big column cap of sectional area 2 in low cost.The lifting employing of column cap 2 vacuumizes the generation negative pressure and promotes, and reduces cost greatly than hydraulic system.Sealing between column cap 2 and the cylinder 1 can compensate automatically.
Claims (3)
1. chromatography separates two-chamber dynamic compression post, and it is characterized in that: have cylinder, column cap is set in the cylinder, is provided with sealing between column cap and the inboard wall of cylinder; Column cap is divided into upper prop chamber and lower prop chamber with cylinder, and the upper prop chamber is provided with charging aperture and vacuum orifice and air inlet, and charging aperture is connected with pipeline with column cap, and the lower prop chamber is provided with into feeding port and liquid outlet, and lower prop is full of filler in the chamber.
2. chromatography as claimed in claim 1 separates two-chamber dynamic compression post; It is characterized in that: the sealing that is provided with between column cap and the inboard wall of cylinder comprises that the top is against the sealing ring that the elastomeric material on the inboard wall of cylinder is processed, and establishes annular recess, the annular seal compensating part that ccontaining elastomeric material is processed in the groove on the sealing ring; Press on the sealing compensation spare and be provided with the sealing ring counterpart; Connect screw and pass the sealing ring counterpart, the lower end snail that connects screw is located on the column cap, connects on the screw to be arranged with the pressure compensation spring; The top, upper end of pressure compensation spring is against on the screw terminal that connects screw, and top, the lower end of pressure compensation spring is against on the sealing ring counterpart.
3. chromatography as claimed in claim 1 separates two-chamber dynamic compression post; It is characterized in that: a counter-force device connects firmly above cylinder; A column cap lock nut spiral shell is located in the logical screw that is provided with on the counter-force device; The upper end of column cap lock nut is provided with handwheel, and the lower end of column cap lock nut is fixedly connected with the column cap guide rod, and the lower end cooperation of column cap guide rod is plugged in the set guide hole of column cap.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210343911.5A CN102836572B (en) | 2012-09-17 | 2012-09-17 | Double-cavity dynamic compression column for chromatographic separation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210343911.5A CN102836572B (en) | 2012-09-17 | 2012-09-17 | Double-cavity dynamic compression column for chromatographic separation |
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| Publication Number | Publication Date |
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| CN102836572A true CN102836572A (en) | 2012-12-26 |
| CN102836572B CN102836572B (en) | 2014-11-05 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201210343911.5A Active CN102836572B (en) | 2012-09-17 | 2012-09-17 | Double-cavity dynamic compression column for chromatographic separation |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103111092A (en) * | 2013-02-04 | 2013-05-22 | 北京创新通恒科技有限公司 | Method for compression of evenly-mixed slurry liquid for liquid chromatography and liquid-phase chromatography column |
| CN103263786A (en) * | 2013-05-24 | 2013-08-28 | 江苏迪沃特仪器设备科技有限公司 | Guard column of industrial-preparation-type chromatographic column |
| CN103691153A (en) * | 2013-12-05 | 2014-04-02 | 成都雅途生物技术有限公司 | Chromatographic column for protein purification |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022197733A1 (en) | 2021-03-16 | 2022-09-22 | Bio-Rad Laboratories, Inc. | Dynamic axial compression for preparative columns using external compression |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1396721A1 (en) * | 2001-05-18 | 2004-03-10 | Northwest University | A caky chromatographic column and the method for producing it and its applications |
| CN2825054Y (en) * | 2005-09-26 | 2006-10-11 | 於红军 | A novel medium-pressure separating chromatographic column |
| CN201894871U (en) * | 2010-11-26 | 2011-07-13 | 大连依利特分析仪器有限公司 | Dynamic axial compression column piston |
| CN102225248A (en) * | 2011-03-29 | 2011-10-26 | 中国科学院过程工程研究所 | Sealing assembly of chromatography column |
-
2012
- 2012-09-17 CN CN201210343911.5A patent/CN102836572B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1396721A1 (en) * | 2001-05-18 | 2004-03-10 | Northwest University | A caky chromatographic column and the method for producing it and its applications |
| CN2825054Y (en) * | 2005-09-26 | 2006-10-11 | 於红军 | A novel medium-pressure separating chromatographic column |
| CN201894871U (en) * | 2010-11-26 | 2011-07-13 | 大连依利特分析仪器有限公司 | Dynamic axial compression column piston |
| CN102225248A (en) * | 2011-03-29 | 2011-10-26 | 中国科学院过程工程研究所 | Sealing assembly of chromatography column |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103111092A (en) * | 2013-02-04 | 2013-05-22 | 北京创新通恒科技有限公司 | Method for compression of evenly-mixed slurry liquid for liquid chromatography and liquid-phase chromatography column |
| CN103263786A (en) * | 2013-05-24 | 2013-08-28 | 江苏迪沃特仪器设备科技有限公司 | Guard column of industrial-preparation-type chromatographic column |
| CN103691153A (en) * | 2013-12-05 | 2014-04-02 | 成都雅途生物技术有限公司 | Chromatographic column for protein purification |
| CN103691153B (en) * | 2013-12-05 | 2016-05-11 | 成都雅途生物技术有限公司 | For the chromatographic column of protein purification |
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| Publication number | Publication date |
|---|---|
| CN102836572B (en) | 2014-11-05 |
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Effective date of registration: 20210701 Address after: No. 723, Chenglu Road, Xiagang street, Jiangyin City, Wuxi City, Jiangsu Province Patentee after: JIANGYIN JINQUE SEPARATION TECHNOLOGY Co.,Ltd. Address before: No. 57, Yujia lane, Shennan village, Shengang Town, Jiangyin City, Wuxi City, Jiangsu Province Patentee before: Yu Hongjun |
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