CN104212710B - Super-pressure cell crushing instrument pressurization homogenization integrated device - Google Patents
Super-pressure cell crushing instrument pressurization homogenization integrated device Download PDFInfo
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- CN104212710B CN104212710B CN201410461557.5A CN201410461557A CN104212710B CN 104212710 B CN104212710 B CN 104212710B CN 201410461557 A CN201410461557 A CN 201410461557A CN 104212710 B CN104212710 B CN 104212710B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
Abstract
The invention discloses a kind of super-pressure cell crushing instrument pressurization homogenization integrated device, comprise long-cylinder, main adapter sleeve, high pressure cylinder homogeneous main body, attached female connector and short oil cylinder, the top of described high pressure cylinder homogeneous main body is provided with the charging aperture being communicated with high pressure chest, described charging aperture is connected with integrated feedstock device, described long-cylinder, main adapter sleeve, high pressure cylinder homogeneous main body, attached female connector and short oil cylinder successively coaxial inner conductor are installed, and the pressured column stopper rod in the high pressure chest of high pressure cylinder homogeneous main body is connected with the piston rod of long-cylinder, homogenizing valve in the inner chamber being communicated with high pressure chest of described high pressure cylinder homogeneous main body is connected with the push rod of short oil cylinder. super-pressure cell crushing instrument of the present invention operates steadily with pressurization homogenization integrated device, and it is little to take up room.
Description
Technical field
The present invention relates to a kind of cell crushing instrument pressurization homogenization device, relate in particular to a kind of super-pressure cell brokenBroken instrument pressurization homogenization integrated device.
Background technology
Cell breaking technology refers to and utilizes outside destroy cell membrane and cell membrane, makes cellular content comprise objectThe technology that product component discharges is synthetic nonsecreting type biochemical substances (product) in separation and purification cellBasis. In conjunction with the major progress on recombinant DNA technology and tissue culture technique, thought in the past and be difficult to obtainProtein now can large-scale production. Develop at present various kinds of cell breaking method, to adapt to notWith purposes and dissimilar cell membrane fragmentation. Breaking method can be advised and be received as Mechanical Method and the large class of on-mechanical method two.And that Mechanical Method equipment used also has is a variety of, and conventional equipment is super high pressure cell crusher, shouldConventional equipment is in biological cell, medicine, food, milk, cosmetics, chemical materials, nano material etc.Fragmentation, fine dispersion, particle nanometer, emulsification processing in tool have been widely used. The pressurization that this is conventionalThe shortcoming that homogeneous device exists is: the liquid outlet of (1) its pressurization homogenization device is driveed on top, and fluid has some setbacks,Easily have residual; (2) Pressure gauge of its pressurization homogenization device is directly connected with high pressure chest, makes high pressure gauge frequentlyNumerous pulsation, thus easily damage; (3) high-pressure energy discharges and easily produces temperature rise; (4) high pressure homogeneous valve holdsEasy to wear. Therefore be necessary to invent a kind of new pressurization homogenization device, to improve aforementioned disadvantages.
Summary of the invention
The object of this invention is to provide a kind of super-pressure cell crushing instrument pressurization homogenization integrated device, structureCompactness, operates steadily, and it is little to take up room, and is beneficial to miniaturization.
For achieving the above object, the invention provides the makeup of a kind of super-pressure cell crushing instrument pressurization homogenization onePut, comprise long-cylinder, main adapter sleeve, high pressure cylinder homogeneous main body, attached female connector and short oil cylinder, described high pressureThe top of cylinder homogeneous main body is provided with the charging aperture being communicated with high pressure chest, and described charging aperture is connected with integrated feedstockDevice, described long-cylinder, main adapter sleeve, high pressure cylinder homogeneous main body, attached female connector and short oil cylinder are same successivelyAxial line is installed, and pressured column stopper rod in the high pressure chest of high pressure cylinder homogeneous main body and the piston rod of long-cylinderConnect the homogenizing valve in the inner chamber being communicated with high pressure chest of described high pressure cylinder homogeneous main body and the push rod of short oil cylinderConnect.
Long-cylinder of the present invention, main adapter sleeve, high pressure cylinder homogeneous main body, attached female connector and short oil cylinder are pressed successivelyCoaxial inner conductor is installed, vibration can reduce work time, operates steadily, and compact conformation, take up roomLittle, be beneficial to miniaturization.
As a further improvement on the present invention, the left side of described high pressure cylinder homogeneous main body is provided with high pressure chest and is communicated withPressure gauge connector, in described Pressure gauge connector, be provided with the first check valve, described high pressure cylinder homogeneous main bodyAlso be provided with and the liquid outlet that sample chamber is communicated with that goes out of high pressure cylinder homogeneous main body one of the valve body of described the first check valvePart be positioned at described high pressure cylinder homogeneous main body and with high pressure cylinder homogeneous main body formation monolithic construction, described in go outLiquid mouth be positioned at the right side of described high pressure cylinder homogeneous main body and with go out sample chamber bottom and be connected, and, described fluidThe axial line of mouth and the angle of horizontal direction are 20 degree. Because a part for the valve body of the first check valve is positioned at heightIn cylinder pressure homogeneous main body and with high pressure cylinder homogeneous main body, form monolithic construction, unidirectional in compared to existing technologyValve, the invention enables the structure of pressurization homogenization integrated device compacter, thereby is conducive to Miniaturization Design,But also can prevent high pressure gauge pulsation, avoid high pressure gauge to damage; Because liquid outlet is positioned at high pressure cylinder homogeneous main bodyRight side connect sample chamber bottom, and the axis of liquid outlet and the angle of horizontal direction be 20 degree, compares existingIn technology, liquid outlet is opened the mode at high pressure cylinder homogeneous main body top, and the fluid resistance of liquid outlet of the present invention moreLittle, fluid is more smooth and easy, is difficult for having residual, and it is also very convenient that drain pipe is installed on liquid outlet, andCan also reduce the length of the drain pipe that connects liquid outlet, thereby reduce the space taking.
Further improve as of the present invention, described in to go out sample chamber be annular, the axial line of described liquid outletTangent with the bottom circumference that goes out sample chamber, this structure can further reduce sample residual quantity.
Further improve as of the present invention, described charging aperture is connected to the tail end top of high pressure chest. TherebyIntegrated feedstock device can be connected to the tail end top of high pressure chest, thereby be convenient to sample introduction and exhaust.
Further improve as of the present invention, described integrated feedstock device comprises the second check valve and bin stockCup, the import of the valve seat of described the second check valve is directly connected with the bottom of described bin stock cup, and described second is singleOutlet to the valve body of valve is connected with described charging aperture, and the valve body of described the second check valve and described bin stockCup is monolithic construction. This structure can make the structure of pressurization homogenization integrated device compacter, therebyBe conducive to Miniaturization Design, and can also reduce the loss of material in fragmentation, and easy to operate.
Further improve as of the present invention, described the first check valve, the second check valve and high pressure cylinder are equalThe axial line of matter main body intersects vertically. This structure is conducive to Pressure gauge and accurately detects the makeup of pressurization homogenization onePressure in putting, and be conducive to Miniaturization Design.
As of the present invention further improve, between the spool of described the first check valve and valve seat for plane connectsTouching sealing, is also plane contact sealing between the spool of described the second check valve and valve seat. This structure processingConvenient, and the good operating stability of the first check valve and the second check valve, long service life.
Further improve as of the present invention, described bin stock cup is provided with bowl cover, and described bowl cover is provided with useIn the quick coupling of Rapid Cleaning. Thereby can avoid material contaminated, and facilitate cleaning operation.
As another kind of improvement of the present invention, the top of described main adapter sleeve offers subjectivity and examines adjustment window,Bottom offers water circulation window, and the top of described attached female connector offers attached observation and adjusts window, and open bottomBe provided with attached water cycle window, observe and adjustment and cooling water circulation to facilitate.
By following description also by reference to the accompanying drawings, it is more clear that the present invention will become, and these accompanying drawings are for explainingEmbodiments of the invention.
Brief description of the drawings
Fig. 1 is the front view of super-pressure cell crushing instrument of the present invention pressurization homogenization integrated device.
Fig. 2 is the left view of super-pressure cell crushing instrument of the present invention pressurization homogenization integrated device.
Fig. 3 is the cutaway view of high pressure cylinder homogeneous main body and the first check valve.
The cutaway view of the integrated feed arrangement of Fig. 4.
Detailed description of the invention
With reference now to accompanying drawing, describe embodiments of the invention, in accompanying drawing, similarly element numbers representative is similarly firstPart.
Please refer to Fig. 1-Fig. 4, super-pressure cell crushing instrument of the present invention comprises with pressurization homogenization integrated deviceArray from left to right and coaxial inner conductor install long-cylinder 1, main adapter sleeve 2, high pressure cylinder homogeneous main body3, attached female connector 4 and short oil cylinder 5. The left end screw thread of described high pressure cylinder homogeneous main body 3 is by described main connectionThe two ends screw thread of cover 2 and the threaded one end of described long-cylinder 1 connect into entirety, described high pressure cylinder homogeneous main body 3Right-hand member screw thread connect into by the two ends screw thread of described attached female connector 4 and the threaded one end of described short oil cylinder 5Entirety.
Further illustrate, the top of described main adapter sleeve 2 offers subjectivity and examines adjustment window 21, bottomOffer water circulation window 22, the top of described attached female connector 4 offers attached observation and adjusts window 41, bottomOffer attached water cycle window 42. Subjectivity examines adjustment window 21 and attached observation adjustment window 41 can conveniently enterRow is observed and is adjusted, and water circulation window 22 and attached water cycle window 42 can facilitate cooling water circulation.
Further illustrate, in the high pressure chest of described high pressure cylinder homogeneous main body 3, have reciprocally mobile pressurizationPlunger rod 31, described pressured column stopper rod 31 stretch out the left end of described high pressure cylinder homogeneous main body 3 and with long-cylinder 1Piston rod connect, described long-cylinder 1 is the master cylinder that pressured column stopper rod 31 moves. Described high pressure cylinder homogeneousIn the inner chamber being communicated with high pressure chest of main body 3, also have homogenizing valve, inlet and the high pressure cylinder of described homogenizing valve are equalThe inner chamber of matter main body 3 is communicated with and is positioned on same axial line, and the push rod 32 of described homogenizing valve and short oil cylinder 5 connectsConnect, described short oil cylinder 5 is attached oil cylinders of controlled fragmentation pressure. Homogenizing valve is placed on to high pressure cylinder homogeneous main body 3Inner chamber in and the inlet of homogenizing valve and the inner chamber of high pressure cylinder homogeneous main body 3 are positioned on same axial lineStructure can make the pressurization of material and homogeneous complete in very short distance, does not need to connect in addition exterior line,Offer convenience to installation and use, can reduce the volume of complete machine simultaneously, be conducive to Miniaturization Design. In addition,The both sides of high pressure cylinder homogeneous main body 3 adopt the mode of two-way cylinder can greatly simplify traditional complete machine structure, subtractFew connector and check valve, reduced fault rate, is convenient to inspection and accessory maintain and replace. Described high pressure cylinder is equalThe left side of matter main body 3 is provided with the Pressure gauge connector being communicated with high pressure chest, in described Pressure gauge connector, is provided withThe first check valve 6. The top of described high pressure cylinder homogeneous main body 3 is provided with the charging aperture being communicated with high pressure chest, described inCharging aperture is connected to the tail end top of high pressure chest, and described charging aperture is connected with integrated feedstock device 7, thereby makesObtain the tail end top that integrated feedstock device 7 is connected to high pressure chest, be convenient to sample introduction and exhaust. Described high pressure cylinderHomogeneous main body 3 is also provided with the liquid outlet 9 that is connected with drain pipe 8, and it is equal that described liquid outlet 9 is positioned at described high pressure cylinderThe right side of matter main body 3 and with go out sample chamber bottom and be connected, and, the axial line of described liquid outlet 9 and levelThe angle of direction is 20 degree, thereby resistance is little, and fluid is smooth and easy, be difficult for having residual, and the installation of drain pipe 8Very convenient. Illustrate further, described in to go out sample chamber be annular, the axial line of described liquid outlet 9Tangent with the bottom circumference that goes out sample chamber, thus sample residual quantity can further be reduced.
Illustrate further, described the first check valve 6 comprises the first valve body 61, the first spool 62 andOne valve seat 63. Described the first spool 62 and described the first valve seat 63 are positioned at the passage of described the first valve body 61,And between described the first spool 62 and the first valve seat 63, be plane contact sealing, thus good operating stability,Long service life. A part for described the first valve body 61 be positioned at high pressure cylinder homogeneous main body 3 and with described high pressureCylinder homogeneous main body 3 forms monolithic construction, and another part is positioned at outside high pressure cylinder homogeneous main body 3, thereby canImprove the stability of pressurization homogenization integrated device, prevent high pressure gauge pulsation, avoid high pressure gauge to damage, andCompact conformation, is conducive to Miniaturization Design.
Described integrated feedstock device 7 comprises the second check valve 71 and bin stock cup 72, the first described check valve6, the axial line of the second check valve 71 and high pressure cylinder homogeneous main body 3 intersects vertically, and is more conducive to Pressure gauge standardReally detect the pressure in pressurization homogenization integrated device, and be conducive to Miniaturization Design. Described the second check valve71 comprise the second valve body 71a, the second spool 71b and the second valve seat 71c. Described the second spool 71b and secondValve seat 71c is positioned at the passage of described the second valve body 71a, and described the second spool 71b and the second valve seat 71cBetween be plane contact sealing, thereby good operating stability, long service life. Described the second valve body 71a withDescribed bin stock cup 72 is monolithic construction. In described the second valve seat 71c, be provided with import 711, described import 711Directly be connected with the bottom of described bin stock cup 72, the outlet of described the second valve body 71a is connected with described charging aperture,Thereby do not need long connecting line, can greatly reduce the loss of sample materials in fragmentation, each broken sampleThe minimum of product only needs 3-5 milliliter; Meanwhile, can directly use draw point when the second check valve 71 exhaustFrom bin stock cup 72, insert exhaust, operation is very easy easily. Described bin stock cup 72 is provided with bowl cover 73,Described bowl cover 73 is connected with 72 sockets of bin stock cup, can be to be also threaded, and adds sample and institute to facilitateWhile stating the second check valve 71 exhaust gas inside, described bowl cover 73 is taken off. Described bowl cover 73 is provided with for fastThe quick coupling 74 cleaning, to facilitate bin stock cup 72 cleaning operations.
In conjunction with most preferred embodiment, invention has been described above, but the present invention is not limited to above announcementEmbodiment, and should contain the various amendments of carrying out according to essence of the present invention, equivalent combinations.
Claims (8)
1. a super-pressure cell crushing instrument pressurization homogenization integrated device, comprise long-cylinder, main adapter sleeve,High pressure cylinder homogeneous main body, attached female connector and short oil cylinder, the top of described high pressure cylinder homogeneous main body is provided with and high pressureThe charging aperture that chamber is communicated with, described charging aperture is connected with integrated feedstock device, it is characterized in that: described lengthOil cylinder, main adapter sleeve, high pressure cylinder homogeneous main body, attached female connector and short oil cylinder successively coaxial inner conductor are installed, andAnd the pressured column stopper rod in the high pressure chest of high pressure cylinder homogeneous main body is connected with the piston rod of long-cylinder, described high pressureHomogenizing valve in the inner chamber being communicated with high pressure chest of cylinder homogeneous main body is connected with the push rod of short oil cylinder, described high pressureThe left side of cylinder homogeneous main body is provided with the Pressure gauge connector being communicated with high pressure chest, in described Pressure gauge connector, establishesHave the first check valve, described high pressure cylinder homogeneous main body be also provided with high pressure cylinder homogeneous main body go out that sample chamber is communicated withLiquid outlet, a part for the valve body of described the first check valve is positioned at described high pressure cylinder homogeneous main body and high pressureCylinder homogeneous main body form monolithic construction, described liquid outlet be positioned at the right side of described high pressure cylinder homogeneous main body and withGo out bottom, sample chamber and be connected, and the axial line of described liquid outlet and the angle of horizontal direction are 20 degree.
2. super-pressure cell crushing instrument pressurization homogenization integrated device as claimed in claim 1, its featureBe: described in to go out sample chamber be annular, the axial line of described liquid outlet is tangent with the bottom circumference that goes out sample chamber.
3. the super-pressure cell crushing instrument pressurization homogenization integrated device as described in 1 or 2 of claims,It is characterized in that: described charging aperture is connected to the tail end top of high pressure chest.
4. super-pressure cell crushing instrument pressurization homogenization integrated device as claimed in claim 3, its featureBe: described integrated feedstock device comprises the second check valve and bin stock cup, the valve seat of described the second check valveImport be directly connected with the bottom of described bin stock cup, the outlet of the valve body of described the second check valve with described in enterMaterial mouthful connects, and the valve body of described the second check valve and described bin stock cup are monolithic construction.
5. super-pressure cell crushing instrument pressurization homogenization integrated device as claimed in claim 4, its featureBe: the axial line of described the first check valve, the second check valve and high pressure cylinder homogeneous main body intersects vertically.
6. the super-pressure cell crushing instrument pressurization homogenization integrated device as described in claim 4 or 5, itsBe characterised in that: between the spool of described the first check valve and valve seat, be plane contact sealing, described second unidirectionalIt between the spool of valve and valve seat, is also plane contact sealing.
7. super-pressure cell crushing instrument pressurization homogenization integrated device as claimed in claim 4, its featureBe: described bin stock cup is provided with bowl cover, and described bowl cover is provided with the quick coupling for Rapid Cleaning.
8. super-pressure cell crushing instrument pressurization homogenization integrated device as claimed in claim 1, its featureBe: the top of described main adapter sleeve offers subjectivity and examines adjustment window, bottom offers water circulation window,The top of described attached female connector offers attached observation and adjusts window, and bottom offers attached water cycle window.
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410461557.5A CN104212710B (en) | 2014-09-11 | 2014-09-11 | Super-pressure cell crushing instrument pressurization homogenization integrated device |
| EP15840792.4A EP3162885B1 (en) | 2014-09-11 | 2015-03-31 | Ultrahigh-pressure homogenizing integrated device and cell disruptor |
| ES15840792.4T ES2688192T3 (en) | 2014-09-11 | 2015-03-31 | Integrated device for ultrahigh pressure homogenization and cell disruptor |
| US15/319,378 US10683481B2 (en) | 2014-09-11 | 2015-03-31 | Ultrahigh-pressure homogenizing integrated device and cell disruptor |
| PCT/CN2015/075445 WO2016037482A1 (en) | 2014-09-11 | 2015-03-31 | Ultrahigh-pressure homogenizing integrated device and cell disruptor |
| JP2017503166A JP6506386B2 (en) | 2014-09-11 | 2015-03-31 | Ultrahigh pressure homogeneous integrated device and cell disrupter |
| DK15840792.4T DK3162885T3 (en) | 2014-09-11 | 2015-03-31 | DEVICE WITH INTEGRATED ULTRA HIGH PRESSURE HOMOGENIZATION AND CELL EXPLOSION UNIT |
| CA2956282A CA2956282C (en) | 2014-09-11 | 2015-03-31 | Integrated device for homogenization and cell disruption featuring a long oil cylinder |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410461557.5A CN104212710B (en) | 2014-09-11 | 2014-09-11 | Super-pressure cell crushing instrument pressurization homogenization integrated device |
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| CN104212710A CN104212710A (en) | 2014-12-17 |
| CN104212710B true CN104212710B (en) | 2016-05-04 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3162885B1 (en) | 2014-09-11 | 2018-07-04 | Guang Zhou Jueng Nano&Bio Technology Co., Ltd | Ultrahigh-pressure homogenizing integrated device and cell disruptor |
| CN104195038B (en) | 2014-09-11 | 2016-06-15 | 广州聚能纳米生物科技股份有限公司 | The double; two high pressure cylinder homogenization integrated device of coaxial line |
| CN105238682B (en) * | 2015-09-24 | 2017-08-11 | 上海化工研究院有限公司 | A kind of device of straight-through Multi-stage piston type ultra high pressure treatment micro-example and application |
| CN107988062A (en) * | 2017-12-28 | 2018-05-04 | 广州聚能纳米生物科技股份有限公司 | A kind of cell breaking plant explosion-protection equipment |
| CN107988063B (en) * | 2017-12-28 | 2023-05-26 | 广州聚能纳米生物科技股份有限公司 | Split type cell disruption structure |
| CN112795483A (en) * | 2021-01-12 | 2021-05-14 | 海南京润珍珠生物技术股份有限公司 | Preparation method of lactobacillus fermentation lysate and cracking device |
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| CN201175652Y (en) * | 2008-04-16 | 2009-01-07 | 广州聚能生物科技有限公司 | Pressurization homogenization integrated device |
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