CN105238682B - A kind of device of straight-through Multi-stage piston type ultra high pressure treatment micro-example and application - Google Patents
A kind of device of straight-through Multi-stage piston type ultra high pressure treatment micro-example and application Download PDFInfo
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- CN105238682B CN105238682B CN201510615661.XA CN201510615661A CN105238682B CN 105238682 B CN105238682 B CN 105238682B CN 201510615661 A CN201510615661 A CN 201510615661A CN 105238682 B CN105238682 B CN 105238682B
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- piston
- oil cylinder
- straight
- bidirectional
- force container
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
Abstract
The present invention relates to a kind of device of straight-through Multi-stage piston type ultra high pressure treatment micro-example and application, the device include stress support (2) and be placed in its bottom hold pending micro-example by force container (5), also include the action tank (1) installed successively along axial line at the top of described stress support (2), bidirectional ram (10) and bidirectional piston oil cylinder, described bidirectional piston oil cylinder sets at least two-stage and is connected and sealed, every grade of bidirectional piston oil cylinder is made up of two-way cylinder and matched double diameter piston respectively, double diameter piston in afterbody bidirectional piston oil cylinder is connected with described by force container (5)；Pressure is produced by action tank (1) motion described in control and transmitted by multi-level piston oil cylinder, finally acts on by force container (5), superelevation pressure is produced, so as to carry out micro-example break process.Compared with prior art, the present invention have the advantages that compact conformation, small volume, not leakiness press.
Description
Technical field
It is super more particularly, to a kind of straight-through Multi-stage piston type the present invention relates to a kind of device for handling micro-example and application
The device of HIGH PRESSURE TREATMENT micro-example and application.
Background technology
During being made substantial progress on recombinant DNA technology and tissue culture technique is combined, and protein can be big
During large-scale production, the nonsecreting type biochemical substances (product) for carrying out isolating and purifying intracellular synthesis are necessary.But separate pure
The basis for changing the nonsecreting type biochemical substances (product) of intracellular synthesis is to carry out clasmatosis.
Cell breaking technology refers to destroy cell membrane and cell membrane using external force, make cellular content include purpose product into
Divide the technology discharged.
During clasmatosis separating-purifying a certain kind protein, first have to a protein and discharged simultaneously from tissue or cell
Original native state is kept, not loss of activity.So to use appropriate method by tissue and clasmatosis.
Processing cell tissue is relatively adapted in view of pressurized treatments or extraction, the infiltration or broken of solvent can be accelerated during pressurization
Bad cell wall, content easily discharges.Be conducive to dissolution equilibrium during pressurize, cell membrane can be burst during explosive decompression.For the above
Principle, it has been proposed that some patents：Such as " number of patent application 200620059198.1, title：A kind of super-pressure clasmatosis dress
Put ", " number of patent application 200620059195.8, title：Super high pressure cell crusher ", " number of patent application
200720309403.X, title：High-pressure cell crusher ", " number of patent application 200920054325.2, title：A kind of two-way list
Shafting oil cylinder biomixer ", " number of patent application 201420521713.8, title：A kind of super-pressure cell crushing instrument is equal with pressurization
Matter integrated apparatus ", " number of patent application 201420521432.2, title：A kind of super-pressure cell crushing instrument ", " patent application
Number 201420658537.2, title：A kind of high pressure cell cracker ", " number of patent application 201410461557.5, title：Superelevation
Press cell crushing instrument pressurization homogenization integrated device " and " number of patent application 201410461556.0, title super-pressure cell
Broken instrument ".But above-mentioned patent is used to be needed to set a variety of pipelines in hydraulic oil pipeline, device, and structure is relative complex, while using one
Level pattern of pressure, if pressing force not enough if clasmatosis can be caused not thorough, influence cellular content acquisition.
The content of the invention
It is an object of the present invention to overcome the above-mentioned drawbacks of the prior art and provide a kind of compact conformation, volume
It is small, occupy little space, not a kind of device of straight-through Multi-stage piston type ultra high pressure treatment micro-example of leakiness pressure and application.
The purpose of the present invention can be achieved through the following technical solutions：
A kind of device of straight-through Multi-stage piston type ultra high pressure treatment micro-example, including stress support and it is placed in its bottom
Hold pending micro-example by force container, it is characterised in that the device is also included at the top of described stress support along axle
Action tank, bidirectional ram and bidirectional piston oil cylinder that heart line is installed successively, described bidirectional piston oil cylinder set at least two-stage
And be connected and sealed, every grade of bidirectional piston oil cylinder is made up of two-way cylinder and matched double diameter piston respectively, last
Double diameter piston in level bidirectional piston oil cylinder is connected with described by force container；
Pressure is produced by the action tank motion described in control and transmitted by multi-level piston oil cylinder, is finally acted on
On by force container, superelevation pressure is produced, so as to carry out micro-example break process.
The device also includes the high-pressure oil pump control loop being connected with described action tank, so that control action exists in real time
It is described that the accurate control of pressure can be realized using control loop by the pressure on force container, improve the reliability of device.
Described stress support is cohesion style turriform stress support, the compact conformation, is occupied little space.
It is connected at the top of described bidirectional ram with described action tank, bottom passes through sealing ring with first order two-way cylinder
Connection, realizes action tank and being tightly connected with the effective of subordinate piston oil-cylinder, so that by the motion of action tank by pressure
Transmit to subordinate's piston oil-cylinder.
Double diameter piston in described multi-level piston oil cylinder is the double diameter piston that head diameter is big, tail diameter is small, real
Show being connected in series for multi-level piston oil cylinder.
Be connected with described bidirectional ram by sealing ring at the top of first order two-way cylinder, remaining two-way cylinder top with
Upper level double diameter piston afterbody is connected by sealing ring；Every grade of two-way cylinder bottom respectively with matched double diameter piston head
Connected by sealing ring；So as to which every grade of two-way cylinder forms sealed oiling chamber, the mode of traditional hydraulic oil pipeline that disappears is taken, is passed through
The mode of series seal connection, and realize by the up and down motion of double diameter piston the hierarchical transmission of pressure.
Hydraulic oil is marked with described oiling chamber.
Described afterbody double diameter piston afterbody is connected and sealed with described by force container by sealing ring, pressure
Super-pressure is produced after transmitting by multi-level piston oil cylinder to act on by force container by afterbody double diameter piston, and using close
The mode of envelope connection prevents pressure leakages, so as to effectively obtain micro-example cellular content.
Described stress support bottom is provided with and the described guide rail by force container is placed for push-and-pull, convenient by force container
Loading and taking-up.
A kind of method of straight-through Multi-stage piston type ultra high pressure treatment micro-example, it is characterised in that this method includes following
Step：
(1) assembling do not hold micro-example by force container 5, carry out device pressure release inspection, handled if pressure release, it is no
Then directly perform step (2)；
(2) load micro-example and assemble by force container 5, open high-pressure oil pump control loop 11, target pressure to be achieved
After force value, setting time is kept, micro-example dissolution equilibrium is realized；
(3) explosive decompression, micro-example cell wall rupture obtains content.
Its theoretical foundation of the invention is pressurized treatments or extracts relatively more suitable to processing cell tissue, can be accelerated during pressurization molten
The infiltration of agent or destruction cell membrane, content easily discharge.Be conducive to dissolution equilibrium during pressurize, can be swollen by cell membrane during explosive decompression
It is broken.Its operation principle is that multi-level piston oil cylinder uses the direct combining form of tandem, by applying pressure F in action tanks, it is double
It is L to plunger motion strokes, double diameter piston, which moves up and down, in multi-level piston oil cylinder carries out pressure transmission, and its stroke is L1、
L2……Ln, afterbody double diameter piston acted on by force container, and its active force is Fn, it can be seen from the equilibrium principle of power, Fs
(Ls+L1+……Ln-1)=FnLn, work as FsWhen smaller, because the superposition of stroke causes (L1+L2+……Ln-1) it is larger, now due to
LnIt is smaller, then it can obtain larger Fm, act on by force container, produce superelevation pressure, the pressure can reach tens thousand of MPa, so that
Break process is carried out to micro-example.
Compared with prior art, the present invention has advantages below：
(1) present apparatus compact conformation, action element takes vertical array mode, and floor space is smaller；
(2) simple for structure, unique double diameter piston structure can be applied directly to down the output power of higher level's motor unit
Level motor unit, the transmission of pressure is amplified in series；
(3) device maintenance and operation is simple, is placed in by force container on guide rail, it is convenient to pulled out from side.
Brief description of the drawings
Fig. 1 leads directly to the structural representation of the device of Multi-stage piston type ultra high pressure treatment micro-example for the present invention.
Wherein, 1 is action tank, and 2 be stress support, and 3 be one-level two-way cylinder, and 4 be two grades of two-way cylinders, and 5 be stress
Container, 6 be guide rail, and 7 be two grades of double diameter pistons, and 8 be one-level double diameter piston, and 9 be sealing ring, and 10 be bidirectional ram, and 11 be high pressure
Oil pump control loop, 12 be hydraulic oil.
Embodiment
The present invention is described in detail with specific embodiment below in conjunction with the accompanying drawings.
Embodiment
As shown in figure 1, a kind of device of straight-through Multi-stage piston type ultra high pressure treatment micro-example, including the He of stress support 2
Be placed in its bottom hold pending micro-example by force container 5, the device also includes from the described top edge of stress support 2
Action tank 1, bidirectional ram 10 and bidirectional piston oil cylinder that axial line is installed successively, described bidirectional piston oil cylinder are set at least
Two-stage is simultaneously connected and sealed, and every grade of bidirectional piston oil cylinder is made up of two-way cylinder and matched double diameter piston respectively, most
Double diameter piston in rear stage bidirectional piston oil cylinder is connected with described by force container 5；Transported by the action tank 1 described in control
Movable property is given birth to pressure and transmitted by multi-level piston oil cylinder, finally acts on by force container 5, produces superelevation pressure, so that
Carry out micro-example break process.The device also includes the high-pressure oil pump control loop 11 being connected with described action tank 1, from
And control action can realize the accurate control of pressure using control loop in the pressure by force container 5 in real time, improve
The reliability of device.
Described stress support 2 is cohesion style turriform stress support, the compact conformation, is occupied little space.Described stress
The bottom of support 2 is provided with and the described guide rail 6 by force container 5 is placed for push-and-pull, convenient loading and taking-up by force container 5.
The described top of bidirectional ram 10 is connected with described action tank 1, bottom and described first order two-way cylinder
Connected by sealing ring 9, being effectively tightly connected for action tank 1 and subordinate piston oil-cylinder is realized, so as to pass through action tank 1
Motion by pressure transmission to subordinate's piston oil-cylinder.Double diameter piston in described multi-level piston oil cylinder be head diameter it is big,
The small double diameter piston of tail diameter, realizes being connected in series for multi-level piston oil cylinder.
Piston oil-cylinder is set to two-stage in the embodiment, and first stage piston oil cylinder includes one-level two-way cylinder 2 and the double footpaths of one-level
Piston 8, first stage piston oil cylinder includes two grades of two-way cylinders 3 and two grades of double diameter pistons 7.The top of described one-level two-way cylinder 2 with
Described bidirectional ram 10 is connected by sealing ring 9, and two grades of tops of two-way cylinder 3 pass through sealing with the afterbody of one-level double diameter piston 8
Circle 9 is connected；The described bottom of one-level two-way cylinder 2 is connected with the head of one-level double diameter piston 8 by sealing ring 9, described two grades
The bottom of two-way cylinder 3 is connected with two grades of heads of double diameter piston 7 by sealing ring 9, and two-stage bidirectional oil cylinder is respectively formed sealed oiling
Chamber.Hydraulic oil 12 is marked with respectively in described oiling chamber.Two grades of described afterbodys of double diameter piston 7 are led to described by force container 5
Cross the realization of sealing ring 9 to be tightly connected, pressure produces super-pressure and passes through two double diameter pistons effect 7 after transmitting by multi-level piston oil cylinder
Pressure leakages are prevented on by force container 5, and by the way of being tightly connected, so as to effectively obtain micro-example cell content
Thing.Described first stage piston oil cylinder and second piston oil cylinder carries out the installation of oiling step by step by the way that hydraulic jacking device auxiliary is lower, can
With higher safety coefficient.By applying pressure F in action tank 1s, the movement travel of bidirectional ram 10 is Ls, two-stage piston oil
Double diameter piston, which moves up and down, in cylinder carries out pressure transmission, and its stroke is respectively L1、L2, afterbody double diameter piston 8 acts on stress
On container 5, its active force is Fn, it can be seen from the equilibrium principle of power, Fs(Ls+L1)=FnL2, work as FsWhen smaller, due to stroke
Superposition causes (Ls+L1) larger, now due to LnIt is smaller, then it can obtain larger Fn, act on by force container 5, produce superelevation
Pressure, the pressure can reach tens thousand of MPa, so as to carry out break process to micro-example.
The method for handling micro-example using above-mentioned straight-through Multi-stage piston type ultra-high pressure apparatus, comprises the following steps：
(1) assembling do not hold micro-example by force container 5, connection high-pressure oil pump control loop 11 reaches goal pressure
Afterwards and keep, by the pressurize of a period of time, if pressure is not revealed and can add micro-example and carry out break process；If pressure
Leakage, need to check each sealing unit, exclude leakage place；
(2) high-pressure oil pump control loop 11 is closed, pressure is released.Take out by force container 5, load the micro of processing to be broken
Sample (contains Escherichia coli, saccharomycete, bacterium etc.).High-pressure oil pump control loop 11 is opened, pressure is transmitted from first stage piston oil cylinder
To second piston oil cylinder, transmit, after target pressure value to be achieved, kept for certain time successively from top to down, it is favourable during pressurize
In dissolution equilibrium.
(3) cell membrane can be burst during explosive decompression, obtain content, complete break process, dismantle each part, cleaning by
Force container 5 is with standby.
Those of ordinary skill in the art is it should be appreciated that the embodiment of the above is intended merely to the explanation present invention's
Limit, as long as in the spirit of the present invention, change, modification to embodiment described above will all fall the present invention's
In Claims scope.
Claims (8)
1. a kind of device of straight-through Multi-stage piston type ultra high pressure treatment sample, including stress support (2) and the Sheng for being placed in its bottom
Put pending sample by force container (5), it is characterised in that the device is also included at the top of described stress support (2) along axle
Action tank (1), bidirectional ram (10) and bidirectional piston oil cylinder that heart line is installed successively, described bidirectional piston oil cylinder set to
Few two-stage is simultaneously connected and sealed, and every grade of bidirectional piston oil cylinder is made up of two-way cylinder and matched double diameter piston respectively,
Double diameter piston in afterbody bidirectional piston oil cylinder is connected with described by force container (5)；
Pressure is produced by action tank (1) motion described in control and transmitted by multi-level piston oil cylinder, is finally acted on
On by force container (5), superelevation pressure is produced, so as to carry out sample broke processing；
Double diameter piston in described multi-level piston oil cylinder is the double diameter piston that head diameter is big, tail diameter is small；
It is connected at the top of first order two-way cylinder with described bidirectional ram (10) by sealing ring (9), at the top of remaining two-way cylinder
It is connected with upper level double diameter piston afterbody by sealing ring (9)；Every grade of two-way cylinder bottom respectively with matched double footpaths
Piston crown is connected by sealing ring (9)；So as to which every grade of two-way cylinder forms sealed oiling chamber.
2. the device of a kind of straight-through Multi-stage piston type ultra high pressure treatment sample according to claim 1, it is characterised in that should
Device also includes the high-pressure oil pump control loop (11) being connected with described action tank (1), so that control action is in institute in real time
State by the pressure on force container (5).
3. a kind of device of straight-through Multi-stage piston type ultra high pressure treatment sample according to claim 1, it is characterised in that institute
The stress support (2) stated is cohesion style turriform stress support.
4. a kind of device of straight-through Multi-stage piston type ultra high pressure treatment sample according to claim 1, it is characterised in that institute
It is connected at the top of the bidirectional ram (10) stated with described action tank (1), bottom passes through sealing ring (9) with first order two-way cylinder
Connection.
5. a kind of device of straight-through Multi-stage piston type ultra high pressure treatment sample according to claim 1, it is characterised in that institute
Hydraulic oil (12) is marked with the oiling chamber stated.
6. a kind of device of straight-through Multi-stage piston type ultra high pressure treatment sample according to claim 4, it is characterised in that institute
The afterbody double diameter piston afterbody stated is connected and sealed with described by force container (5) by sealing ring (9).
7. a kind of device of straight-through Multi-stage piston type ultra high pressure treatment sample according to claim 1, it is characterised in that institute
Stress support (2) bottom stated, which is provided with, places the described guide rail (6) by force container (5) for push-and-pull.
8. the application of the straight-through Multi-stage piston type ultra high pressure treatment sampling device as described in any in claim 1-7, its feature
It is, comprises the following steps：
(1) assembling do not hold sample by force container (5), carry out device pressure release inspection, handled if pressure release, otherwise directly
Perform step (2)；
(2) load sample and assemble by force container (5), open high-pressure oil pump control loop (11), target pressure value to be achieved
Afterwards, setting time is kept, sample dissolution equilibrium is realized；
(3) explosive decompression, sample cell wall rupture, obtains content.
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| CN201510615661.XA CN105238682B (en) | 2015-09-24 | 2015-09-24 | A kind of device of straight-through Multi-stage piston type ultra high pressure treatment micro-example and application |
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| CN201510615661.XA CN105238682B (en) | 2015-09-24 | 2015-09-24 | A kind of device of straight-through Multi-stage piston type ultra high pressure treatment micro-example and application |
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| CN105238682A CN105238682A (en) | 2016-01-13 |
| CN105238682B true CN105238682B (en) | 2017-08-11 |
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| CN111921224B (en) * | 2020-06-12 | 2021-10-29 | 漳州职业技术学院 | Nitrite scavenging additive production line and preparation method thereof |
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Address after: 200062 Shanghai city Putuo District Yunling Road No. 345 Co-patentee after: Shanghai Chemical Institute Testing Co., Ltd. Patentee after: Shanghai Chemical Research Institute Co., Ltd. Address before: 200062 Shanghai city Putuo District Yunling Road No. 345 Co-patentee before: Shanghai Tianke Chemical Detection Co., Ltd. Patentee before: Shanghai Chemical Research Institute Co., Ltd. |
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