CN102500135B - Fast dynamic compression chromatography column - Google Patents
Fast dynamic compression chromatography column Download PDFInfo
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- CN102500135B CN102500135B CN201110324734.1A CN201110324734A CN102500135B CN 102500135 B CN102500135 B CN 102500135B CN 201110324734 A CN201110324734 A CN 201110324734A CN 102500135 B CN102500135 B CN 102500135B
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- end cover
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- dynamic compression
- chromatography column
- column jecket
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Abstract
The invention discloses a fast dynamic compression chromatography column comprising a cylindrical column tube with openings on both ends; both ends of the column tube are respectively provided with an upper end cover and a lower end cover which close the column tube; a piston is arranged in the middle of the column tube; a hose passes through the upper end cover and the piston and is communicated to the inner cavity of the column tube which is close to one side of the lower end cover; and the fast dynamic compression chromatography column is characterized in that: a pressurize opening and a pressure relief opening which are penetrated are arranged on the upper end cover; the pressurize opening is connected with liquid rejection equipment through a pipeline; and the pressure relief opening is provided with a safe valve. The fast dynamic compression chromatography column adopts a hydraulic method to drive the piston to move and maintain the pressure. Compared with a motor, the fast dynamic compression chromatography column has the advantages of more stable power, higher uniformity, low cost, long service life, high column efficiency, good symmetry, good repeatability and the like on the premise of maintaining rather good separation effect with an analytical column, and can be applied to a chromatography column with large diameter.
Description
Technical field
The invention belongs to chemical field, be specifically related to a kind of chromatographic column.
Background technology
Along with developing rapidly of the industries such as pharmacy, biochemical industry, Preparative Liquid Chromatography obtains development and application more and more widely, has become the important method of separated and purifying complex mixture, is particularly useful for the separation of natural products.For making sample obtain effective separation, the filling of chromatographic column must meet the requirement of continuity, uniformity, stability and the compactness of bed., there is sizable difficulty in the filling of the major diameter post of the filling of chromatographic column, especially commercial production scale, according to conventional method, loads large diameter chromatographic column, and bed is unstable, can reduce separating property.
Dynamic axial compression technology is called for short DAC, is in the chromatographic column of various scales, to keep the technology of good separation effect, is also the column packing technique of current preparative chromatography field the best.The core technology of dynamic axial compression column be by piston move up and down fill post, maintain post and press and unload post, piston periphery has been equipped with the sealing ring of particular design can allow that piston easy on and off slides, and can keep high sealing pressing again simultaneously.Applied range as: the separation of extracted form natural plant, synthetic drug, proteins and peptides preparation is also the indispensable means of the modernization of Chinese medicine.At present, the piston movement in this chromatographic column and pressure maintain and substantially all adopt motor to realize by screw mandrel.Because motor cost is high, and the vibrations that produce during machine operation, cause the less stable of chromatographic column, screw mandrel life-span pressure short, that produce inhomogeneous; This also makes it can not be applied to the occasion of the chromatographic column that diameter is larger.
Summary of the invention
The invention provides a kind of solution of the above problems, a kind of working stability, pressure is even, cost is low quick dynamic compression chromatography column are provided.
Technical scheme of the present invention is a kind of quick dynamic compression chromatography column, it comprises the column jecket of cylindrical shape both ends open, described column jecket two ends are respectively arranged with upper end cover and the bottom end cover of described column jecket sealing, in the middle of described column jecket, be provided with piston, one flexible pipe is communicated in the inner chamber near the described column jecket of described bottom end cover one side through described upper end cover and piston, it is characterized in that: on described upper end cover, be provided with the pressurization mouth and the pressure relief opening that all connect, described pressurization mouthful pipeline connects liquid filing provision, and described pressure relief opening is provided with safety valve.
The Pressure gauge of the described column jecket chamber pressure of monitoring preferably, is set on described upper end cover.
Preferably, a described pressurization mouthful inner side is provided with check valve.
Preferably, the exhaust outlet of perforation is set on described upper end cover, the described exhaust outlet outside plug that has removably been threaded.
Preferably, on described piston, be provided with the sealing device of the described column jecket bore seal of its both sides.
Preferably, described sealing device comprises and being arranged near the L shaped sealing ring of described upper end cover one side with near the O-ring seals of described bottom end cover one side.
Preferably, on described bottom end cover, be provided with the solvent outlet running through.
Preferably, described upper end cover and bottom end cover are all removably connected on described column jecket by bolt.
A kind of quick dynamic compression chromatography column of the present invention adopts the mode driven plunger activity of hydraulic pressure and maintains pressure.The advantages such as compare motor and drive, it is keeping, under the prerequisite of the separating effect suitable with analytical column, also having power more stable, and more even, cost is low, the life-span is long, post is imitated high, symmetry and reproducible, can be applied to the chromatographic column that diameter is large.
Accompanying drawing explanation
Fig. 1 is the top view of a kind of quick dynamic compression chromatography column of the present invention;
Fig. 2 be in Fig. 1 A-A to the front view of analysing and observe;
Fig. 3 be in Fig. 1 B-B to the front view of analysing and observe.
The specific embodiment
Below the specific embodiment of the present invention is described in further detail.
As shown in Figure 1 to Figure 3, a kind of quick dynamic compression chromatography column of the present invention comprises the column jecket 10 of cylindrical shape both ends open.Column jecket 10 two ends are respectively arranged with upper end cover 12 and bottom end cover 14, upper end cover 12 and bottom end cover 14 all by bolt detachably by column jecket 10 closed at both ends.In the middle of column jecket 10, being provided with piston 16, one flexible pipes 18 is communicated near in column jecket 10 inner chambers of bottom end cover 14 1 sides through upper end cover 12 and piston 16.On upper end cover 12, be provided with pressurization mouth 20 and pressure relief opening 22.Pressurization mouthful 20 pipelines connect liquid filing provision (not shown), the measuring pump that can directly use liquid chromatogram to use.Pressure relief opening 22 is provided with safety valve 24, and safety valve 24 can be adjusted safe pressure by demand.Pressurization mouthful 20 inner sides are provided with check valve 26.The Pressure gauge 28 of monitoring column jecket 10 chamber pressures is set, with instant gaging pressure on upper end cover 12.The exhaust outlet 30 of perforation is set, the exhaust outlet 30 outsides plug 32 that has removably been threaded on upper end cover 12.The hermetically-sealed construction of piston 16 adds one deck O-ring seals 36 by the L shaped sealing ring 34 of one deck and forms, the liquid of 34 pairs of tops of L shaped sealing ring plays a good sealing effectiveness, below O-ring seals 36 stops solvent upper reaches, below, below filler is played to a cleaning effect simultaneously, make below condiment be unlikely to enter between L shaped sealing ring 34 and the tube wall of column jecket 10, impact sealing.On bottom end cover 14, be provided with the solvent outlet 38 running through.
Its method of operating is:
1. piston 16 is packed in column jecket 10 inner chambers;
2. upper end cover 12 is installed on column jecket 10, installs suitable safety valve 24 simultaneously;
3. reversing column jecket 10 packs filler (not shown) in column jecket 10;
4. bottom end cover 14 is installed on column jecket 10;
5. open the plug 32 of exhaust outlet 30, at pressurization mouth 20, by measuring pump, inject solvent, until gas is emptying, add upper plug head 32;
6. continue to inject solvent, observe Pressure gauge 28 readings, pressure reaches after desired value, closes measuring pump;
7. the joint 40 of pressurization mouth 20 and measuring pump is backed out, be then screwed in colvent inlet, by flexible pipe 18, in column jecket 10 inner chambers, inject solvent, solvent carries out mask work under the effect of filler.
It is hydraulic pressure that piston movement of the present invention and pressure maintain what lean on, hydraulic power is more stable, more even, makes that it has that cost is low, the life-span is long, post effect is high, symmetry and reproducible feature, can load diameter range large, and the maintenance separating effect suitable with analytical column.
Above embodiment is only the present invention's a kind of embodiment wherein, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (5)
1. a quick dynamic compression chromatography column, it comprises the column jecket of cylindrical shape both ends open, described column jecket two ends are respectively arranged with upper end cover and the bottom end cover of described column jecket sealing, in the middle of described column jecket, be provided with piston, one flexible pipe is communicated near in the described column jecket inner chamber of described bottom end cover one side through described upper end cover and piston, it is characterized in that: on described upper end cover, be provided with the pressurization mouth and the pressure relief opening that all connect, described pressurization mouthful pipeline connects liquid filing provision, and described pressure relief opening is provided with safety valve; The Pressure gauge of the described column jecket chamber pressure of monitoring is set on described upper end cover; A described pressurization mouthful inner side is provided with check valve; The exhaust outlet of perforation is set, the described exhaust outlet outside plug that has removably been threaded on described upper end cover.
2. a kind of quick dynamic compression chromatography column according to claim 1, is characterized in that: on described piston, be provided with the sealing device of the described column jecket bore seal of its both sides.
3. a kind of quick dynamic compression chromatography column according to claim 1, is characterized in that: described sealing device comprises and being arranged near the L shaped sealing ring of described upper end cover one side with near the O-ring seals of described bottom end cover one side.
4. a kind of quick dynamic compression chromatography column according to claim 1, is characterized in that: on described bottom end cover, be provided with the solvent outlet running through.
5. a kind of quick dynamic compression chromatography column according to claim 1, is characterized in that: described upper end cover and bottom end cover are all removably connected on described column jecket by bolt.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110324734.1A CN102500135B (en) | 2011-10-24 | 2011-10-24 | Fast dynamic compression chromatography column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110324734.1A CN102500135B (en) | 2011-10-24 | 2011-10-24 | Fast dynamic compression chromatography column |
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| Publication Number | Publication Date |
|---|---|
| CN102500135A CN102500135A (en) | 2012-06-20 |
| CN102500135B true CN102500135B (en) | 2014-01-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201110324734.1A Active CN102500135B (en) | 2011-10-24 | 2011-10-24 | Fast dynamic compression chromatography column |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102989199B (en) * | 2012-12-11 | 2014-09-03 | 聊城万合工业制造有限公司 | Inner storage constant-pressure preparative column device |
| CN103263786A (en) * | 2013-05-24 | 2013-08-28 | 江苏迪沃特仪器设备科技有限公司 | Guard column of industrial-preparation-type chromatographic column |
| CN105004806B (en) * | 2015-07-08 | 2017-04-05 | 浙江省海洋水产研究所 | Concertina type spiral combination type immune affinity column |
| CN109358147A (en) * | 2018-12-25 | 2019-02-19 | 成都格莱精密仪器有限公司 | A kind of chromatographic column that can quickly load |
| CN110384951A (en) * | 2019-04-10 | 2019-10-29 | 江苏汉邦科技有限公司 | A kind of novel heat insulation chromatographic column |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0762118A2 (en) * | 1995-09-12 | 1997-03-12 | K.D. PHARMA GmbH | Chromatography column |
| CN2906618Y (en) * | 2006-05-02 | 2007-05-30 | 江苏汉邦科技有限公司 | Dynamic axial compression chromatographic column |
| CN200948371Y (en) * | 2006-07-07 | 2007-09-19 | 陈开明 | Axial pressure adding type preparation and chromatography pole |
| CN202289557U (en) * | 2011-10-24 | 2012-07-04 | 利穗科技(苏州)有限公司 | Rapid and dynamic compression chromatography column |
-
2011
- 2011-10-24 CN CN201110324734.1A patent/CN102500135B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0762118A2 (en) * | 1995-09-12 | 1997-03-12 | K.D. PHARMA GmbH | Chromatography column |
| CN2906618Y (en) * | 2006-05-02 | 2007-05-30 | 江苏汉邦科技有限公司 | Dynamic axial compression chromatographic column |
| CN200948371Y (en) * | 2006-07-07 | 2007-09-19 | 陈开明 | Axial pressure adding type preparation and chromatography pole |
| CN202289557U (en) * | 2011-10-24 | 2012-07-04 | 利穗科技(苏州)有限公司 | Rapid and dynamic compression chromatography column |
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| CN102500135A (en) | 2012-06-20 |
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