CN102989199B - Inner storage constant-pressure preparative column device - Google Patents
Inner storage constant-pressure preparative column device Download PDFInfo
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- CN102989199B CN102989199B CN201210533037.1A CN201210533037A CN102989199B CN 102989199 B CN102989199 B CN 102989199B CN 201210533037 A CN201210533037 A CN 201210533037A CN 102989199 B CN102989199 B CN 102989199B
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Abstract
The invention relates to an inner storage constant-pressure preparative column device, and provides a miniaturization device. The device comprises a bracket, a preparative column and a hydraulic cylinder. The hydraulic cylinder is vertically mounted on the bracket, and a U notch for fixing the preparative column is arranged at a distance under the hydraulic cylinder; and the hydraulic cylinder acts on the preparative column and drives the preparative column to compress. The preparative column has functions of automatic inner column pressure compensation and automatic column bed defect repair, and also can ensure constant pressure state of packing in the column. The device is especially suitable for development and research on different target products and different processes in a laboratory, so as to greatly reduce development costs, and improve development efficiency.
Description
Technical field
The present invention relates to high performance liquid preparative chromatography technical field, specifically a kind of novel interior energy storage constant voltage preparative column device.
Background technology
High performance liquid chromatography technology of preparing is a kind of isolation technics that can obtain high-purity compound, many pharmaceuticals researchers wish oneself to have a set of preparative chromatography equipment, to carry out the development of production technology and technique for applying on chemico-analytic basis.
With analyzing, chromatogram is the same, and chromatographic column is the core component of chromatographic system.The post bed height of modern high performance liquid preparative chromatography post generally equates (being generally 20-25cm) with conventional analysis post, and main purpose is the difficulty of amplifying from analyzing the manufacturability of preparation in order to reduce.At present, in preparative chromatography industry, applying more is dynamic axial compression column (DAC), in use, piston dependence in chromatographic column is attached thereto the hydraulic jack connecing and produces all the time a relatively constant pressure compression post bed fillers, eliminates at any time in elution process because the dead space that filler particles relatively moves and produces.Dynamic axial compression column is used good, can meet the purifies and separates requirement of a lot of target products, and maximum advantage is to be applicable to large-scale industrial production.But the manufacturability for the product in laboratory research stage is separated, the dynamic axial compression column thick heaviness that seems, inconvenient operation, and also expensive.Because under lab, different objects, different processes, needs different fillers as the mutually fixing or fixing carrier of phase.
Interior energy storage constant voltage preparative column is developed in order to meet the need just, before this this type not on market.
Summary of the invention
The object of the present invention is to provide a kind of small-sized high performance liquid preparative chromatography column device, to reduce production costs under the prerequisite guaranteeing product yield and purity, for the separation and purification of products technical study in laboratory and pilot scale stage provides easy appointed condition, be particularly useful in the smaller situation in space.
The technical solution adopted for the present invention to solve the technical problems is:
This interior energy storage constant voltage preparative column device, comprises support, preparative column and hydraulic jack, it is characterized in that, the vertical state of described hydraulic jack is rack-mount, and a distance is provided with a fixedly U-shaped breach for preparative column below described hydraulic jack; Described hydraulic jack acts on preparative column and drives preparative column to compress.
Further, described preparative column by column jecket, piston, energy-stored spring group, column jecket upper press cover and post at the bottom of flange form, the two ends up and down of described column jecket are respectively equipped with flange at the bottom of column jecket upper press cover and post, described piston is plugged in column jecket, and on the piston head coordinating with column jecket, be provided with energy-stored spring assembly, on flange at the bottom of described piston and post, be respectively equipped with sieve plate at the bottom of the piston sieve plate that is oppositely arranged and post, at the bottom of described post, on flange, be provided with out liquid interface.
Described column jecket top is provided with the snap ring projection of a ring-type, for coordinating with U-shaped breach.
The hand oil pump of controlling hydraulic jack is fixed on bracket base.
Further, described support comprises horizontally disposed base, upper backup pad and lower supporting plate, between described upper and lower support plate, by column, connects, and hydraulic jack is arranged on upper backup pad, U-shaped breach is positioned on lower supporting plate, and by C shape rod member, connects between lower supporting plate and base.
Described energy-stored spring assembly is a rubber seal.
The invention has the beneficial effects as follows:
1) adopt energy-stored spring group structure to replace the hydraulic jack in dynamic axial compression column, storage power when dress post, had both guaranteed the constant pressure in post bed, had guaranteed separating effect, greatly reduce equipment operating cost simultaneously, be applicable to the research and development of laboratory and pilot-scale.
2) a special-purpose packing column machine, can load the interior energy storage constant voltage preparative column of multiple different size, also can load different types of filler, is more suitable for the separating technology requirement of different products and different scales.
3) dress post process is simple to operation, and the post bed accessory after filling has with DAC post and is close to identical effect, but with low cost, especially easily obtains rapid promotion and application.
In sum, interior energy storage constant voltage preparative column has auto-compensation post internal pressure, and the function of self-regeneration post bed defect guarantees the pressure constant state of filler in post simultaneously.Be specially adapted in laboratory the developmental research to different target products and different process, greatly reduce R&D costs, improve development efficiency.
Accompanying drawing explanation
Fig. 1 is the full sectional view of preparative column;
Fig. 2 is schematic perspective view of the present invention;
Fig. 3 is filler process schematic diagram;
Fig. 4 is for installing preparative column process schematic diagram;
Fig. 5 prepares look post process schematic diagram for compressing.
In figure: 11 bases, 12 upper backup pads, 13 lower supporting plates, 14U v notch v, 15C shape rod member, 16 columns, 2 hydraulic jacks, 21 hand oil pumps, 3 preparative columns, 31 column jeckets, 311 snap ring projections, 32 pistons, 33 energy-stored spring groups, 34 column jecket upper press covers, flange at the bottom of 35 posts, 351 go out liquid interface, 36 piston rods, 37 piston sieve plates, sieve plate at the bottom of 38 posts, 4 fillers, 5 homogenate buckets.
The specific embodiment
As shown in Fig. 1～Fig. 5,
This interior energy storage constant voltage preparative column device, comprises support, preparative column and hydraulic jack.
Support comprises horizontally disposed base 11, upper backup pad 12 and lower supporting plate 13, between upper and lower support plate, by three root posts 16, connects, and upper backup pad is circular, and hydraulic jack 2 is arranged on the centre of upper backup pad 12, in vertical state.In lower supporting plate 13 centre, be provided with U-shaped breach 14, this U-shaped breach is positioned at hydraulic jack below certain distance, and this distance is less than the sphere of action of hydraulic jack, for fixing preparative column 3.Between lower supporting plate 13 and base 11, by some C shape rod members 15, connect, C shape rod member is outstanding backward, can obtain larger operating space like this.The hand oil pump 21 of controlling hydraulic jack 2 is also fixed on bracket base 11, convenient operation and make full use of space, small and exquisite practicality in structure.
Preparative column 3 by column jecket 31, piston 32, energy-stored spring group 33, column jecket upper press cover 34, post at the bottom of flange 35 form, column jecket 31 is a cylindrical body, and top, column jecket top is provided with the snap ring projection 311 of a ring-type, for coordinating with U-shaped breach 14, column jecket inside is filling space, for filler 4.The two ends up and down of column jecket 31 are respectively equipped with flange 35 at the bottom of column jecket upper press cover 34 and post, can be by securing member fastening or clamping element clamp, form the upper and lower end face of column jecket.Piston 32 is plugged in column jecket 31, piston 32 is arranged on piston rod 36, piston rod 36 tops can be connected with hydraulic jack 2 piston rods, and on the piston head coordinating with column jecket, be provided with energy-stored spring assembly 33, described energy-stored spring assembly is a rubber seal, can seal and provide pretension frictional force.On flange at the bottom of piston and post, being respectively equipped with sieve plate 38 at the bottom of the piston sieve plate 37 that is oppositely arranged and post, being provided with out liquid interface 351 on flange at the bottom of post 35, is a through hole.
Due to the effect of energy-stored spring assembly, by parameter reasonable in design, make the pressure at filler position and frictional force reach balance, can realize the object of constant voltage.When the pressure in column jecket changes, the hydraulic jack perseveration on top, has auto-compensation post internal pressure, the function of self-regeneration post bed defect.
Interior energy storage constant voltage preparative column device dress post process is: the filler 4 that homogenate is good packs homogenate bucket 5 into, then inject fast in column jecket 31, the column jecket 31 that homogenate is housed is put into rapidly on dress column system together with piston rod, wherein column jecket snap ring projection coordinates with the U-shaped breach of lower supporting plate, guarantees column jecket 31 and piston rod, hydraulic jack concentric simultaneously; Now operate the handle on hand oil pump 21, make hydraulic cylinder piston rod descending, the filler in compacting column jecket is observed oil circuit Pressure gauge simultaneously, controls the pressure in post bed, after post bed internal pressure stable equilibrium, screws in column jecket upper press cover 34.After standing 20 minutes, take off constant voltage preparative column, sealing liquid import and export, prepares to use.
Embodiment recited above is described the preferred embodiment of the present invention; not scope of the present invention is limited; design under the prerequisite of spirit not departing from the present invention; relevant technical staff in the field, to various distortion of the present invention and improvement, all should expand in protection domain as determined in the claims in the present invention book.
Claims (3)
1. in, an energy storage constant voltage preparative column device, comprises support, preparative column and hydraulic jack, it is characterized in that, the vertical state of described hydraulic jack is rack-mount, and a distance is provided with a fixedly U-shaped breach for preparative column below described hydraulic jack; Described hydraulic jack acts on preparative column and drives preparative column to compress;
Described preparative column by column jecket, piston, energy-stored spring group, column jecket upper press cover and post at the bottom of flange form, the two ends up and down of described column jecket are respectively equipped with flange at the bottom of column jecket upper press cover and post, described piston is plugged in column jecket, and on the piston head coordinating with column jecket, be provided with energy-stored spring assembly, on flange at the bottom of described piston and post, be respectively equipped with sieve plate at the bottom of the piston sieve plate that is oppositely arranged and post, at the bottom of described post, on flange, be provided with out liquid interface; And described column jecket top is provided with the snap ring projection of a ring-type, coordinate with U-shaped breach;
Described support comprises horizontally disposed base, upper backup pad and lower supporting plate, between described upper and lower support plate, by column, connect, described hydraulic jack is arranged on upper backup pad, and described U-shaped breach is positioned on lower supporting plate, and by C shape rod member, connects between lower supporting plate and base.
2. interior energy storage constant voltage preparative column device according to claim 1, is characterized in that, the hand oil pump of controlling hydraulic jack is fixed on bracket base.
3. interior energy storage constant voltage preparative column device according to claim 1, is characterized in that, described energy-stored spring assembly is a rubber seal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210533037.1A CN102989199B (en) | 2012-12-11 | 2012-12-11 | Inner storage constant-pressure preparative column device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210533037.1A CN102989199B (en) | 2012-12-11 | 2012-12-11 | Inner storage constant-pressure preparative column device |
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| Publication Number | Publication Date |
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| CN102989199A CN102989199A (en) | 2013-03-27 |
| CN102989199B true CN102989199B (en) | 2014-09-03 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103267149B (en) * | 2013-05-07 | 2015-09-30 | 荣捷生物工程（苏州）有限公司 | Automatic dress column valve |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822914A (en) * | 2010-04-23 | 2010-09-08 | 聊城万合工业制造有限公司 | High-efficiency liquid-phase preparation device of axial pressurizing chromatographic column |
| CN101954202A (en) * | 2009-07-16 | 2011-01-26 | 中国科学院化学研究所 | Constant-pressure closed ascending chromatographic column device and application method thereof |
| CN202136832U (en) * | 2010-11-23 | 2012-02-08 | 孙俊 | Normal pressure column chromatography device under closed environment |
| CN102500135A (en) * | 2011-10-24 | 2012-06-20 | 利穗科技(苏州)有限公司 | Fast dynamic compression chromatography column |
| CN203090528U (en) * | 2012-12-11 | 2013-07-31 | 聊城万合工业制造有限公司 | Internal energy-storing constant pressure preparative column apparatus |
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2012
- 2012-12-11 CN CN201210533037.1A patent/CN102989199B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101954202A (en) * | 2009-07-16 | 2011-01-26 | 中国科学院化学研究所 | Constant-pressure closed ascending chromatographic column device and application method thereof |
| CN101822914A (en) * | 2010-04-23 | 2010-09-08 | 聊城万合工业制造有限公司 | High-efficiency liquid-phase preparation device of axial pressurizing chromatographic column |
| CN202136832U (en) * | 2010-11-23 | 2012-02-08 | 孙俊 | Normal pressure column chromatography device under closed environment |
| CN102500135A (en) * | 2011-10-24 | 2012-06-20 | 利穗科技(苏州)有限公司 | Fast dynamic compression chromatography column |
| CN203090528U (en) * | 2012-12-11 | 2013-07-31 | 聊城万合工业制造有限公司 | Internal energy-storing constant pressure preparative column apparatus |
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