CN102487744A - Industrialized implementing method for breaking dormant state of flaccid anemone rhizome and application thereof - Google Patents
Industrialized implementing method for breaking dormant state of flaccid anemone rhizome and application thereof Download PDFInfo
- Publication number
- CN102487744A CN102487744A CN2011103931637A CN201110393163A CN102487744A CN 102487744 A CN102487744 A CN 102487744A CN 2011103931637 A CN2011103931637 A CN 2011103931637A CN 201110393163 A CN201110393163 A CN 201110393163A CN 102487744 A CN102487744 A CN 102487744A
- Authority
- CN
- China
- Prior art keywords
- ground
- industrialized
- crow
- anemone rhizome
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to the technical field of agriculture plant cultivation and particularly relates to an industrialized implementing method for breaking a dormant state of flaccid anemone rhizome and an application thereof. The flaccid anemone rhizome is also named as Anemone flaccida Fr.Schmidt and is a wild medicinal plant. In order to solve the problems that the growth cycle of the wild flaccid anemone rhizome is short, the output is small, the multiplication coefficient is low, and the like, the invention provides the industrialized implementing method for efficiently relieving the dormant state of the flaccid anemone rhizome, and the method can be used for efficiently increasing the output and increasing the multiplication coefficient. The industrialized implementing method comprises the following specific steps: picking the flaccid anemone rhizome in September; soaking the root stocks of the flaccid anemone rhizome into a gibberellin solution; washing with running water; placing at a moist part at an elevation of 1100m-3000m in a big basin; keeping the surface of the flaccid anemone rhizome wet and ensuring no accumulated water in the basin; growing tender leaves before or after the 25th days; and after sprouting, transplanting to the field for planting. According to the industrialized implementing method, a technical support for increasing the output of the flaccid anemone rhizome planted in an industrialized form is provided.
Description
Technical field
The invention belongs to agricultural plant culture technique field, relate to a kind of industrialized implementation method and application of breaking the black resting state in ground.
Technical background
The ground crow, the shady anemone (Anemone flaccida Fr.Schmidt) of formal name used at school woods, another name land mine, unregistered land thunder, golden beading, centipede pseudo-ginseng, flaccid anemone etc.; Belong to Ranunculaceae Anemone (Ranunculaceae Anemone.), perennial herb can be used as medicine after rhizome dries, and mainly is distributed in each province, the middle and lower reach of Yangtze River, is grown in the dark and damp place of hillside sylvan life of height above sea level 1100 to 3000m.All on the books, warm in nature " China's book on Chinese herbal medicine ", " national Chinese herbal medicine compilation ", " Guizhou folk medicine ", " the temmoku Chinese yam is planted will in Zhejiang " etc., flavor is hot, little hardship, and wind-damp dispelling helps muscles and bones, treatment of rheumatalgia pain, traumatic injury.
Pharmaceutical research shows, active ingredient---the triterpene saponin material of ground crow, mainly contain clearing heat and detoxicating, the antalgic and sedative effect; Non-specific immune function and specific cellular immunity, humoral immunity all there is significant inhibitory effect.The anemone capsule of being processed by Rhizoma Anemones flaccidae extract has the effect of wind-damp dispelling, strengthening the bones and muscles, swelling and pain relieving, can be used for treating various arthralgias and traumatic injury, and has no side effect.
In west area, Chinese Hubei, wildly crow emerges annual mid-March, late May acrial part withered gradually, get into resting stage subsequently, begin to regrow to March next year.The ground crow is short vegetative period, and poor growth yields poorly, and reproduction coefficient is low, still manque at present industrialization plantation.Wildly crow is according to its self-sow rule, and just be fit to gather the vegetative period of generally taking 5 years.
Under field conditions (factors), every strain ground crow has only 1～2 blade, and ground crow root-like stock rudiment is few, the general terminal bud germination growth accumulation output of only leaning on.Because the ground crow grows slowly, yields poorly, and adds excavation blindly, wild resource can't satisfy the Chinese patent drug development requirement, and does not realize the industrialization plantation of ground crow at present.
Summary of the invention
The present invention is a kind of industrialized implementation method and application of breaking the black resting state in ground; Concrete grammar is: behind September extraction ground crow; Ground crow root-like stock is soaked in Gibberellins solution; Clean with running water then, place the shady place of height above sea level 1100m to 3000m, keep no ponding in black surface wettability in ground and the basin.Be transplanted to the big Tanaka of subtropics high mountain humid climate after the sprouting, keep self-sow.
After the present invention breaks the black resting state in ground, realized that ground crow grows twice in 1 year, effectively shortened growth cycle, foreshortened to 2 years the vegetative period in 5 years, improved the industrialization plantation efficient of ground crow greatly by crow wildly.
On the industrialization planting technology of ground crow, the present invention has more practicality and operability.
Realization approach of the present invention:
The annual ground crow root-like stock extraction that will be in resting state September cleans, reject wherein rot with disease is arranged; Gibberellins solution (being preferably 100mg/L) with 0～150mg/L is soaked 4～24 hours (being preferably 14 hours); Clean 3～4 times with running water then, be placed in the big basin, be placed in the dark and damp environment of the high mountain of height above sea level 1100m to 3000m; Keep no ponding in black surface wettability in ground and the basin; But the crow of the insight about 25 days bud-end all puts forth new leaves, and transplants the big Tanaka of the high mountain humid climate to the subtropics, keeps self-sow.
Advantage of the present invention:
1, crow began to get into resting stage in late May, and the present invention can effectively remove its resting state through the immersion of using Gibberellins solution, and it can anti-season be grown in the fall, realized two seasons of annual growth, effectively improved output, shortened growth cycle;
Though 2, the correlative study of the black tissue culture technique in ground in the at present existing laboratory; Utilize breadboard temperature and hormone means can realize the anti-season growth of ground crow, still, the influence factor of laboratory operating method is more; Cost is high, can not be used for industrialized implementation; The present invention has simplified the step of removing the black resting state in ground, is applicable to large batch of artificial planting.
3, simple, convenient, the easy operating of the present invention is applicable to planting site crow in enormous quantities, industrialization.
Description of drawings
Fig. 1: for ground crow among the present invention is soaked 14 hours sprouting situation behind the breaking dormancy in the Gibberellins solution of 100mg/L;
Fig. 2: for ground crow among the present invention is soaked 14 hours grown in field situation behind the breaking dormancy in the Gibberellins solution of 100mg/L.
Embodiment
Embodiment 1
Material: wildly black root-like stock (variety type: Ranunculaceae, Anemone, the shady anemone Anemone of woods flaccida fr.schmidt)
Reagent: 920 gibberellin (Shanghai Tongrui Biotechnology Co., Ltd., lot number: 070508), absolute ethyl alcohol (analyzing pure), purified water
The compound method of Gibberellins solution: take by weighing 50,100 respectively, each three parts of 920 gibberellin of 150mg, add an amount of absolute ethyl alcohol, dissolving adds purified water and is diluted to 1L, promptly get 50,100, respectively three parts of the Gibberellins solution of 150mg/L, and is subsequent use.
Method of operating: on September 5th, 2009 Hubei governor sun county The Cloud Terrace famine is adopted next wildly black root-like stock, the about 10cm of length, size is evenly.At first rinse well, placed one hour, after the variable concentrations Gibberellins solution of pressing table 1 design is soaked with running water; Clean 3～4 times with running water, be put in respectively in the different big basins, big basin is put in the local dark and damp environment; Water spray is preserved moisture, no ponding in black feasiblely surface wettability and the basin.Grow a large amount of tender leafs after the time about 25 days, but the insight crow sprouted, transplant extremely local big Tanaka, the local subtropics high mountain humid climate that belongs to, dense fog condenses naturally and moistens soil, need not man-made irrigation, keeps self-sow.
Take black root-like stock processing in different Gibberellins solution immersions ground and germinating thereof as shown in table 1.
The gibberellin treatment result of table 1 variable concentrations
The result: as shown in table 1, adopt Gibberellins solution to soak the black root-like stock in ground, along with the increase of Gibberellins solution concentration, germination rate significantly raises, and when concentration was 100mg/L, germination rate reached 100%; Soak time prolongs, and germination rate significantly raises, and soaks 14 hours and 24 hours germination rates are 100%, consider that the minimizing soak time can raise the efficiency, so soak time is preferably 14 hours.
Embodiment 2
Get the ground crow root-like stock 50kg that gibberellin 100mg/L solution soaking was handled in 14 hours, be transferred to the big Tanaka's plantation that is fit to environment after handling by the method for embodiment 1.
Home environment: big Tanaka smooth herein or that the gradient is less is planted in the plantation land for growing field crops on the about 1800 meters high mountain of long positive height above sea level.
Soil condition: field soil is a cinnamon soil, and soil nutrient content is an organic matter about 2.5%, full nitrogen 0.23%, available phosphorus 12.49ppm, available potassium 312.32ppm, pH value 6.7-7.1.
Weather conditions: about 1800 meters of base height above sea level, the cold summer in winter is cool, and fog fills the air throughout the year, and winter, heavy snow has sealed the mountain passes, belongs to typical subtropics high mountain humid climate.Ground crow vegetative period, to by the end of May, the atmosphere average daily temperature was 11.53 ℃ in vegetative period at late March, relative humidity of atomsphere 60%-99%, and late March is to by the end of May the about 300mm of rainfall.
Key elements such as the soil in land for growing field crops, water quality, atmosphere all meet national relevant regulations standard through the detection of legal relevant departments.
Implantation time: on September 30th, 2009
Planting density and mode: the unified mode that adopts row to plant, according to line-spacing 20cm trench digging, ditch depth 5-8cm, spacing in the rows 10cm puts into the ground crow root-like stock of sprouting uniformly, and a black root-like stock in ground is put at every place, and earthing compresses compacting immediately.Planting density is about 33000 sections/mu.
Fertilising: fertilizer is a rapeseed cake fertilizer 50.0kg/ mu, and chemical fertilizer is urea 13.0kg/ mu, superphosphate 41.7kg/ mu, potassium sulphate 9.3kg/ mu.
Before ground crow plantation trench digging, the rapeseed cake fertilizer that will become thoroughly decomposed earlier is sprinkled upon big field uniformly, will pass through well-mixed urea, superphosphate and potassium sulphate fertilizer then and be sprinkled upon big field uniformly, beats the field with rotovator again.
Irrigate and method: the land for growing field crops weather belongs to subtropics high mountain humid climate, and dense fog condenses naturally and moistens soil, need not man-made irrigation.
Damage by disease and insect and the control of ground crow: temporarily also find the black damage by disease and insect that takes place in ground.
Gather: behind the black seedling in ground, the ground crow of promptly can gathering.Gathered in July, 2010 in the crow land for growing field crops, ground of this embodiment.
Output: the fresh ground crow of gathering is removed earth, weighs 183.4kg.
Embodiment 3
Get the gibberellin blank solution and soak the ground crow root-like stock 50kg that handled in 14 hours, be transferred to the big Tanaka's plantation that is fit to environment after handling by the method for embodiment 1.
Home environment: big Tanaka smooth herein or that the gradient is less is planted in the plantation land for growing field crops on the about 1800 meters high mountain of long positive height above sea level.
Soil condition: field soil is a cinnamon soil, and soil nutrient content is an organic matter about 2.5%, full nitrogen 0.23%, available phosphorus 12.49ppm, available potassium 313.32ppm, pH value 6.7-7.1.
Weather conditions: about 1800 meters of base height above sea level, the cold summer in winter is cool, and fog fills the air throughout the year, and winter, heavy snow has sealed the mountain passes, belongs to typical subtropics high mountain humid climate.Late March is to by the end of May, and the atmosphere average daily temperature is 11.53 ℃, relative humidity of atomsphere 60%-99%, the about 300mm of rainfall.
Key elements such as the soil in base, water quality, atmosphere all meet national relevant regulations standard through the detection of legal relevant departments.
Implantation time: on September 30th, 2009
Planting density and mode: the unified mode that adopts row to plant, according to line-spacing 20cm trench digging, ditch depth 5-8cm, spacing in the rows 10cm puts into the ground crow root-like stock of sprouting uniformly, and a black root-like stock in ground is put at every place, and earthing compresses compacting immediately.Planting density is about 33000 sections/mu.
Fertilising: fertilizer is a rapeseed cake fertilizer 50.8kg/ mu, and chemical fertilizer is urea 13.0kg/ mu, superphosphate 41.2kg/ mu, potassium sulphate 9.0kg/ mu.
Before ground crow plantation trench digging, the rapeseed cake fertilizer that will become thoroughly decomposed earlier is sprinkled upon big field uniformly, will pass through well-mixed urea, superphosphate and potassium sulphate fertilizer then and be sprinkled upon big field uniformly, beats the field with rotovator again.
Irrigate and method: the land for growing field crops weather belongs to subtropics high mountain humid climate, and dense fog condenses naturally and moistens soil, need not man-made irrigation.
Damage by disease and insect and the control of ground crow: temporarily also find the black damage by disease and insect that takes place in ground.
Gather: behind the black seedling in ground, the ground crow of promptly can gathering.Gathered July in the crow land for growing field crops, ground of this embodiment.
Output: the fresh ground crow of gathering is removed earth, weighs 96.7kg.
Embodiment 4
Material: wildly black root-like stock (variety type: Ranunculaceae, Anemone, the shady anemone Anemone of woods flaccida fr.schmidt)
Reagent: 920 gibberellin (Shanghai Tongrui Biotechnology Co., Ltd., lot number: 070508), NAA (NAA, Shanghai thing Science and Technology Ltd. of suitable Johnson & Johnson, lot number: 080215), absolute ethyl alcohol (analyzing pure), purified water
The compound method of prescription solution:
Take by weighing four parts of 920 gibberellin, every part of 100mg, subsequent use.Take by weighing four parts of NAA, be respectively 0.5,1.0,1.5,2.0mg, every part of gibberellin that adding has weighed up adds an amount of absolute ethyl alcohol, and dissolving adds purified water and is diluted to 1L, promptly gets the Gibberellins solution of 100mg/L and the NAA mixed solution of variable concentrations, and is subsequent use.
Method of operating: on September 3rd, 2009, the ground crow root-like stock 50kg that gathers and planted in 2009 rinses well with running water; Placed one hour, and used the Gibberellins solution of 100mg/L and NAA (NAA) mixed solution of variable concentrations to soak after 14 hours, clean 3～4 times with running water; Be put in respectively in the different big basins; Big basin is put in the dark and damp environment of local high mountain, and water spray is preserved moisture, no ponding in black feasiblely surface wettability and the basin.Grew a large amount of tender leafs after the time about 25 days, but the insight crow sprouted, transplant the big Tanaka of the high mountain humid climate to the subtropics, keep self-sow, on June 15th, 2010 gathered.
Take the ground crow root-like stock disposition of processing of NAA mixed solution of Gibberellins solution and variable concentrations of 100mg/L as shown in table 2.
The Gibberellins solution of table 2 100mg/L and the mixed information slip of the NAA of variable concentrations
The result: increase the growth of root behind the adding NAA, output does not have marked change.NAA is plant growth regulator commonly used, and working concentration generally is not more than 2mg/L.
Embodiment 5
On September 6th, 2010, the wildly black root-like stock 50kg that gathers rinses well with running water; Placed one hour, and used the NAA mixed solution of 100mg/L Gibberellins solution and 1.5mg/L to soak after 14 hours, clean 3～4 times with running water; Be put in respectively in the different big basins; Big basin is put in the dark and damp environment of local high mountain, and water spray is preserved moisture, no ponding in black feasiblely surface wettability and the basin.Grew a large amount of tender leafs after the time about 25 days, but the insight crow sprouted, transplant the big Tanaka of high mountain humid climate, keep self-sow to the subtropics.
Home environment: grow on the about 1800 meters high mountain of positive height above sea level, plant big Tanaka smooth herein or that the gradient is less.
Soil condition: field soil is a cinnamon soil, and soil nutrient content is an organic matter about 2.2%, full nitrogen 0.28%, available phosphorus 11.98ppm, available potassium 310.02ppm, pH value 6.7-7.1.
Weather conditions: about 1800 meters of base height above sea level, the cold summer in winter is cool, and fog fills the air throughout the year, and winter, heavy snow has sealed the mountain passes, belongs to typical subtropics high mountain humid climate.Late March is to by the end of May, and the atmosphere average daily temperature is 11.53 ℃, relative humidity of atomsphere 60%～99%, the about 300mm of rainfall.
Key elements such as the soil in base, water quality, atmosphere all meet national relevant regulations standard through the detection of legal relevant departments.
Implantation time: on September 28th, 2010
Planting density and mode: the unified mode that adopts row to plant, according to line-spacing 20cm trench digging, ditch depth 5-8cm, spacing in the rows 10cm puts into the root-like stock that the ground crow sprouts uniformly, and the root-like stock that a ground crow sprouts is put at every place, and earthing compresses compacting immediately.Planting density is about 33000 sections/mu.
Fertilising: fertilizer is a rapeseed cake fertilizer 50.4kg/ mu, and chemical fertilizer is urea 12.7kg/ mu, superphosphate 40.8kg/ mu, potassium sulphate 9.7kg/ mu.
Before ground crow plantation trench digging, the rapeseed cake fertilizer that will become thoroughly decomposed earlier is sprinkled upon big field uniformly, will pass through well-mixed urea, superphosphate and potassium sulphate fertilizer then and be sprinkled upon big field uniformly, beats the field with rotovator again.
Irrigate and method: the base weather belongs to subtropics high mountain humid climate, and dense fog condenses naturally and moistens soil, need not man-made irrigation.
Damage by disease and insect and the control of ground crow: temporarily also find the black damage by disease and insect that takes place in ground.
Gather: on June 18th, 2011 gathered.
Output: the fresh ground crow of gathering is removed earth, weighs 163.6kg.
Claims (4)
1. an industrialized implementation method and application of breaking the black resting state in ground, its characteristic is being: take Gibberellins solution to soak the black root-like stock in ground ground crow extraction September, breaks its resting state; With the running water flushing, in big basin, place environment dark and damp on the mountain of height above sea level 1100～3000m, keep no ponding in black surface wettability in ground and the basin; The bud-end of about 25 world crow puts forth new leaves, and transplants to big Tanaka, makes ground crow self-sow in the fall, improves output.
2. according to claim 1, take Gibberellins solution to soak the black root-like stock in ground, wherein Gibberellins solution comprises that Gibberellins solution perhaps contains the prescription solution of gibberellin.
3. according to claim 2, take to contain the black root-like stock in prescription solution soaking ground of gibberellin, comprise the gibberellin and the mixed solution of one or more plant growth regulator preparations in addition.
4. according to claim 2, take the black root-like stock in gibberellin pure solution soaking ground to break its resting state, wherein, the concentration of Gibberellins solution is 0～150mg/L, is preferably 100mg/L; Soak time is 4～24 hours, is preferably 14 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103931637A CN102487744A (en) | 2011-12-01 | 2011-12-01 | Industrialized implementing method for breaking dormant state of flaccid anemone rhizome and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103931637A CN102487744A (en) | 2011-12-01 | 2011-12-01 | Industrialized implementing method for breaking dormant state of flaccid anemone rhizome and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102487744A true CN102487744A (en) | 2012-06-13 |
Family
ID=46179663
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011103931637A Pending CN102487744A (en) | 2011-12-01 | 2011-12-01 | Industrialized implementing method for breaking dormant state of flaccid anemone rhizome and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102487744A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115997624A (en) * | 2022-10-25 | 2023-04-25 | 东北林业大学 | Transplanting domestication method for anemone nigrum |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101243741A (en) * | 2008-03-17 | 2008-08-20 | 华中农业大学 | Method for relieving Diwu rhizome dormancy |
| CN101390496A (en) * | 2008-09-28 | 2009-03-25 | 华中农业大学 | Vitro quick di-wu breeding method |
-
2011
- 2011-12-01 CN CN2011103931637A patent/CN102487744A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101243741A (en) * | 2008-03-17 | 2008-08-20 | 华中农业大学 | Method for relieving Diwu rhizome dormancy |
| CN101390496A (en) * | 2008-09-28 | 2009-03-25 | 华中农业大学 | Vitro quick di-wu breeding method |
Non-Patent Citations (5)
| Title |
|---|
| 庄德斌，杜春艳: "林荫银莲花及丰产栽培技术", 《中国林副特产》 * |
| 张忠池 等: "林荫银莲花组织培养研究", 《中草药》 * |
| 李佩华: "赤霉素处理马铃薯整薯休眠效应的研究", 《贵州农业科学》 * |
| 罗士韦: "《植物激素》", 31 March 1963, 上海科学技术出版社 * |
| 蒋德勋: "赤霉素对黄连种子萌发及植株生长的影响", 《植物生理学通讯》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115997624A (en) * | 2022-10-25 | 2023-04-25 | 东北林业大学 | Transplanting domestication method for anemone nigrum |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101926257B (en) | Ecological compound planting method of Paris polyphylla | |
| CN103283450B (en) | Golden camellia bud seedling stock grafting propagation method | |
| CN105684708B (en) | A method of in karst region, rejected reasons interplant Chinese medicine using three-dimensional composite mode | |
| CN102771284B (en) | Ecological cultivation method for houttuynia cordata | |
| CN103262748A (en) | GAP (Good Agricultural Practices) cultivation method for medicinal gynostemma pentaphylla under mountain land red earth forests | |
| CN105210642B (en) | Control method of the Yunnan Rhizoma Paridis rhizome cutting propagation infection with improving planting benefit | |
| CN104025863B (en) | Use the method that super enriching plant improves Radix Achyranthis Bidentatae chrysanthemum cadmium pollution soil repair ability | |
| CN105660298A (en) | Method for interplanting Paris polyphylla in star anise woodland | |
| CN104770263A (en) | Mixed planting method of lemons and tendril-leaved fritillary bulb | |
| CN113491223A (en) | Method for breeding thesium Chinese seeds | |
| CN111727793A (en) | Stereoscopic cultivation method for crude drug tsaoko in karst mountain land | |
| CN102934550A (en) | Spinulose tree fern propagation and transplant method | |
| CN113207535B (en) | Method for growing seedlings of bruguiera gymnorrhiza | |
| CN110810100B (en) | Method for sowing, seedling raising and afforestation of photinia serrulata in winter | |
| CN104584832B (en) | Method for transplanting taxus chinensis seedlings and interplanting corns | |
| CN101779567B (en) | Standardized planting method for cephalotaxus hainanensis | |
| CN105850468A (en) | Planting method of Paris polyphylla | |
| CN1994044A (en) | Method for planting Chinese herb triperygium wilfordii | |
| CN101554115B (en) | Method for culturing Chinese silvergrass with high yield | |
| CN112931134B (en) | Davidia involucrata relocation protection technology | |
| CN111631087B (en) | Method for rapidly fixing carbon in alpinia katsumadai forest | |
| CN108781967A (en) | Evodia rutaecarpa and woaded blue stereo ecological implantation methods | |
| CN102487744A (en) | Industrialized implementing method for breaking dormant state of flaccid anemone rhizome and application thereof | |
| CN113229030A (en) | Method for interplanting oil peonies in orchard | |
| CN115228921B (en) | Method for repairing light and medium cadmium-polluted paddy field soil by utilizing polygonum hydropiper |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120613 |