CN101243741A - Method for relieving Diwu rhizome dormancy - Google Patents
Method for relieving Diwu rhizome dormancy Download PDFInfo
- Publication number
- CN101243741A CN101243741A CNA200810047075XA CN200810047075A CN101243741A CN 101243741 A CN101243741 A CN 101243741A CN A200810047075X A CNA200810047075X A CN A200810047075XA CN 200810047075 A CN200810047075 A CN 200810047075A CN 101243741 A CN101243741 A CN 101243741A
- Authority
- CN
- China
- Prior art keywords
- diwu
- rhizomes
- dormancy
- rhizome
- wild
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a method to break the dormancy of Diwu rhizomes, belonging to the technical field of agricultural plant cultivation. Diwu is also called Anemone flaccida Fr.Schmidt, which is a wild Chinese herbal medicine plant. The invention solves the problem that wild Diwu has short growth cycle, and small biomass and propagation coefficient. The invention provides an effective method to break the dormancy of Diwu rhizomes, which is characterized in that: in each year, from the first ten days of June to the middle ten days of November, the Diwu rhizomes are soaked in 6-benzylaminopurine having the concentration of 1-20mg/L for 20 hours to 24 hours; then the Diwu rhizomes are washed by running water for three or four times, put in a plastic box equipped with yellow sand substrate, and cultivated under the temperature ranging from 4 DEG C to 20 DEG C; finally the Diwu rhizomes are transplanted under artificial light or natural light. The method has an advantage that, the invention provides technical support to the improvement of Diwu biological yield, off-season culture and the conversion from wild to artificial.
Description
Technical field
The invention belongs to agricultural plant culture technique field, be specifically related to a kind of method of relieving Diwu rhizome dormancy.
Background technology
The ground crow, the shady anemone (Anemone flaccida Fr.Schmidt) of formal name used at school woods, Ranunculaceae Anemone (Anemone L.), perennial herb, can be used as medicine after rhizome dries, mainly be distributed in each province, the middle and lower reach of Yangtze River, be grown in the dark and damp place of hillside sylvan life of height above sea level 1500～3000m.In Chinese Hubei province, emerge annual mid-March, and the death gradually of late May acrial part enters resting stage subsequently, and vegetative period is short, poor growth, and biological yield is little, and seminal propagation power is low, does not still have tame report at present.
Pharmaceutical research is found, the active ingredient of ground crow-triterpene saponin material, mainly contain clearing heat and detoxicating, the antalgic and sedative effect; Non-specific immune function and specific cellular immunity, humoral immunity all there is significant inhibitory effect.The anemone capsule of being made by Rhizoma Anemones flaccidae extract has the effect of wind-damp dispelling, strengthening the bones and muscles, swelling and pain relieving, can be used for treating various arthralgias and traumatic injury, and has no side effect.Because the ground crow grows slowly, yields poorly, and adds excavation blindly, wild resource can't satisfy the Chinese patent drug development requirement, and under field conditions (factors), every strain ground crow has only 1-2 blade, the black root-like stock rudiment in ground is few, and is general only by terminal bud germination growth accumulation biological yield.
Summary of the invention
The invention provides a kind of method of utilizing hormone and the effective relieving Diwu rhizome dormancy of temperature, provide technical support for improving black biomass in ground and off-season cultivation.
The present invention is achieved in that
In the early June-mid-November in every year, Mei Nian early November preferably, to be in the ground crow root-like stock of resting state, rinse well with running water, reject wherein rot and disease is arranged, soak behind the 20-24h (preferably with 1-20mg/L 6-benzyl aminopurine (6-BA), 6-BA concentration is 10-20mg/L), again with running water flushing 3-4 time, place in the plastic casing of yellow sand of the bacterium of going out (according to conventional sterilizing methods: 100 ℃ of high steams are sterilization 20h down), cover the gauze of preserving moisture above the plastic casing, place (8-16 ℃ of 4-20 ℃ day-night constant temperature or alternating temperature, use climatic cabinate, 16 ℃ of daytimes, 8 ℃ of evenings)) cultivate under the condition, keep the skin wet every day in right amount, the terminal bud lateral bud of processed rhizome of Rhizoma Anemones flaccidae is all sprouted after one week, they is transplanted in the culturing room of artificial lighting (intensity of illumination is about 2000lux) or natural lighting afterwards.
Compare with existing method, the present invention has the following advantages:
1, crow enters resting stage in June, and the present invention can effectively remove its dormancy by the processing of hormone and temperature, makes its anti-season be grown to serve as possibility.
2, adopt the present invention can make the black every strain number of blade in ground be increased to the 3-5 sheet, increased its photosynthetic area, effectively improved the annual biological yield of ground crow.
3, adopt the present invention can accelerate the sprouting speed of ground crow, overcome the shortcoming that black germination rate is low under the natural conditions, sprouting is slow.
Description of drawings
Fig. 1: be the sprouting situation after 20mg/L6-BA processing back is to the black breaking dormancy in resting state ground among one of them embodiment of the present invention;
Fig. 2: be the growing state after 20mg/L6-BA processing back is to the black breaking dormancy in resting state ground among one of them embodiment of the present invention.
Embodiment
Embodiment 1
Processing method: on June 8th, 2007 was to November 15, the root-like stock of crow wildly of Hubei governor sun county bronze drum bag will be picked up from, after under running water, rinsing well, after 6-benzyl aminopurine (6-BA) the immersion 24h of variable concentrations is set by table 1, running water flushing 3～4 times, place in the plastic casing in the yellow sand of high-temperature sterilization (according to conventional sterilizing methods, i.e. 100 ℃ of high steams sterilization 20h down), on cover the multilayer gauze and preserve moisture.Then, place 4 ℃ (day-night constant temperatures) as table 1 design, 8 ℃-16 ℃ (with 16 ℃ of climatic cabinate daytimes, 8 ℃ of evenings), 20 ℃ (day-night constant temperature) to sprout down respectively, keep the skin wet every day in right amount.The terminal bud lateral bud of processed rhizome of Rhizoma Anemones flaccidae is all sprouted after one week, they is transplanted in the culturing room of artificial lighting (intensity of illumination is about 2000lux) or natural lighting afterwards.12 rhizomes of every processing are treated to contrast with distilled water.Per 3 days black terminal bud germination numbers in investigation ground, sprouting of lateral bud number, terminal bud begin the to sprout diameter and the length of back 20 days investigation terminal buds, lateral bud.The invention effect sees Table 2-4.
The HORMONE TREATMENT concentration of table 1 relieving Diwu rhizome dormancy and treatment temperature design
The influence that the black over the ground root-like stock of table 2 different temperatures and HORMONE TREATMENT is sprouted
The size of terminal bud, lateral bud under table 3 different temperatures and the HORMONE TREATMENT
Indicate: terminal bud size=terminal bud diameter * terminal bud length lateral bud size=lateral bud diameter * lateral bud length
Table 2 is as can be known: under 4 ℃-20 ℃ temperature, the root-like stock that the 6-BA of variable concentrations handles all can be removed the terminal bud dormancy rapidly, and germination rate all reaches 100%.From terminal bud diameter and length, at 4 ℃, 8 ℃-16 ℃ (alternating temperatures), under 20 ℃, the optium concentration that 6-BA handles is to be 5mg/L, 20mg/L, 20mg/L successively; This shows that along with the rising of temperature, the optium concentration that adopts 6-BA to remove the root-like stock dormancy also is the trend of raising.
The terminal bud size is the most important index of the black trunk diameter growth in relation ground, and table 4 The results of analysis of variance shows that for the influence of terminal bud size, temperature, hormone and the effect of doing mutually of the two thereof all reach utmost point significance level.Wherein, under 8 ℃-16 ℃ (alternating temperatures) and 20 ℃, adopting the 6-BA of 20mg/L to handle 24h is the best approach that promotes the black terminal bud germination in ground, growth.
The action effect variance analysis of the black over the ground terminal bud size of table 4 temperature and 6-BA
Claims (2)
1, the method for relieving Diwu rhizome dormancy, its step comprises cleans ground crow root-like stock, reject wherein rot, the rhizome of disease, it is characterized in that, with the root-like stock of ground crow the early June-mid-November in every year, behind 1-20mg/L6-benayl aminopurine immersion 20-24h, again with running water flushing 3-4 time, place in the plastic casing of yellow sand of the bacterium of going out the covering gauze of preserving moisture above the plastic casing, place under constant temperature or the alternating temperature 4-20 ℃ condition and cultivate, keep the skin wet every day in right amount.
2, method according to claim 1 is characterized in that, the time of described processing is early November.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810047075XA CN101243741B (en) | 2008-03-17 | 2008-03-17 | Method for relieving Diwu rhizome dormancy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810047075XA CN101243741B (en) | 2008-03-17 | 2008-03-17 | Method for relieving Diwu rhizome dormancy |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101243741A true CN101243741A (en) | 2008-08-20 |
| CN101243741B CN101243741B (en) | 2011-01-26 |
Family
ID=39944606
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200810047075XA Active CN101243741B (en) | 2008-03-17 | 2008-03-17 | Method for relieving Diwu rhizome dormancy |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101243741B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101390496B (en) * | 2008-09-28 | 2011-04-20 | 华中农业大学 | Vitro quick di-wu breeding method |
| CN102301886A (en) * | 2011-07-19 | 2012-01-04 | 屯留县民康中药材开发有限公司 | Method for cultivating dried rhizome of rehmannia |
| CN102487744A (en) * | 2011-12-01 | 2012-06-13 | 广州康和药业有限公司 | Industrialized implementing method for breaking dormant state of flaccid anemone rhizome and application thereof |
| CN102487687A (en) * | 2011-12-01 | 2012-06-13 | 广州康和药业有限公司 | Method for planting flaccid anemone rhizome in industrialized form and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100374012C (en) * | 2003-11-07 | 2008-03-12 | 云南省农业科学院园艺作物研究所 | Tissure culture and fast growth of buttercup flowers |
-
2008
- 2008-03-17 CN CN200810047075XA patent/CN101243741B/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101390496B (en) * | 2008-09-28 | 2011-04-20 | 华中农业大学 | Vitro quick di-wu breeding method |
| CN102301886A (en) * | 2011-07-19 | 2012-01-04 | 屯留县民康中药材开发有限公司 | Method for cultivating dried rhizome of rehmannia |
| CN102487744A (en) * | 2011-12-01 | 2012-06-13 | 广州康和药业有限公司 | Industrialized implementing method for breaking dormant state of flaccid anemone rhizome and application thereof |
| CN102487687A (en) * | 2011-12-01 | 2012-06-13 | 广州康和药业有限公司 | Method for planting flaccid anemone rhizome in industrialized form and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101243741B (en) | 2011-01-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103444530B (en) | Technology for field return-to-nature ex-situ plantation of wild anoectochilus roxburghii (Wall.) Lindl in Fujian | |
| CN105165616B (en) | A kind of method of efficiently quick breeding radix tetrastigme test tube seedling | |
| CN105309311A (en) | Method for breeding improved variety of scrophularia ningpoensis Hemsl. | |
| CN103404397A (en) | Method for preventing and treating candidatus liberibacter asiaticus by heat treatment | |
| CN103190343A (en) | Key technology of organic additive for roxburgh anoectochilus terminal bud industrialization intermediate propagation | |
| CN101243741B (en) | Method for relieving Diwu rhizome dormancy | |
| CN108935047B (en) | Ecological planting method for dendrobium | |
| CN103404437B (en) | Novel method for tissue culture rapid propagation of acer paimatum | |
| CN109302985A (en) | The method of external the rataria induction and plant regeneration of sub- lotus | |
| KR100981181B1 (en) | culturing method for burrower bivalve | |
| CN103270860B (en) | A kind of implantation methods of dendrobium candidum | |
| CN105746124A (en) | Labor-saving high-yield gastrodia elata planting method | |
| Monostori et al. | A review on sweet potato with special focus on hungarian production: utilization, biology and transplant production | |
| CN112119815B (en) | Method for preventing and treating trelago pterocarpus in mangrove forest | |
| CN113557912A (en) | Method for interplanting epimedium sagittifolia and economic forest trees | |
| CN101766172B (en) | Application of pteryxin in inducing rice blast and cold resistance of rice | |
| CN106345796A (en) | Method for repairing middle/light cadmium-polluted soil by utilizing perennial energy triarrhena sacchariflora and application thereof | |
| CN101743909A (en) | Tissue culture and propagation method of illicium plant illicium benryi dies | |
| CN107896987B (en) | Breeding method of Guangxi wild adinandra nitida tissue culture seedlings | |
| CN109928504A (en) | A method of utilizing saururus chinensis purifying eutrophication water body | |
| CN109042325A (en) | The tissue cultivating method of rag wood | |
| CN1095320C (en) | Fast root propagating and high-yield cultivation method of Elaegnus umbellata | |
| Thakur et al. | Effect of seed priming on germination characteristics of seeds of Podophyllum hexandrum Royle: An endangered medicinal plant of western Himalaya | |
| CN109463210B (en) | Cultivation method for rapidly harvesting Acanthus ilicifolius roots | |
| De | Indian Lotus-A Multipurpose Aquatic Ornamental Plant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: GUANGZHOU KANGHE PHARMACEUTICAL CO., LTD. Effective date: 20120313 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20120313 Address after: 430070 Wuhan, Hongshan Province, lion street, No. 1 District, No. Co-patentee after: Guangzhou Kanghe Pharmaceutical Co., Ltd. Patentee after: Huazhong Agricultural University Address before: 430070 Wuhan, Hongshan Province, lion street, No. 1 District, No. Patentee before: Huazhong Agricultural University |
|
| ASS | Succession or assignment of patent right |
Free format text: FORMER OWNER: GUANGZHOU KANGHE PHARMACEUTICAL CO., LTD. Effective date: 20131031 Owner name: GUANGZHOU KANGHE PHARMACEUTICAL CO., LTD. Free format text: FORMER OWNER: HUAZHONG AGRICULTURAL UNIVERSITY Effective date: 20131031 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 430070 WUHAN, HUBEI PROVINCE TO: 511440 GUANGZHOU, GUANGDONG PROVINCE |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20131031 Address after: Panyu District of Guangzhou City, Guangdong province 511440 lotus road Shilou town No. 113 Patentee after: Guangzhou Kanghe Pharmaceutical Co., Ltd. Address before: 430070 Wuhan, Hongshan Province, lion street, No. 1 District, No. Patentee before: Huazhong Agricultural University Patentee before: Guangzhou Kanghe Pharmaceutical Co., Ltd. |