CN101243741B

CN101243741B - Method for relieving Diwu rhizome dormancy

Info

Publication number: CN101243741B
Application number: CN200810047075XA
Authority: CN
Inventors: 杨特武; 李宏飞; 耿明建; 朱端卫; 张忠池; 黄漫翔; 方贤东
Original assignee: Huazhong Agricultural University
Current assignee: Guangzhou Kanghe Pharmaceutical Co., Ltd.
Priority date: 2008-03-17
Filing date: 2008-03-17
Publication date: 2011-01-26
Anticipated expiration: 2028-03-17
Also published as: CN101243741A

Abstract

The invention relates to a method to break the dormancy of Diwu rhizomes, belonging to the technical field of agricultural plant cultivation. Diwu is also called Anemone flaccida Fr.Schmidt, which is a wild Chinese herbal medicine plant. The invention solves the problem that wild Diwu has short growth cycle, and small biomass and propagation coefficient. The invention provides an effective methodto break the dormancy of Diwu rhizomes, which is characterized in that: in each year, from the first ten days of June to the middle ten days of November, the Diwu rhizomes are soaked in 6-benzylaminopurine having the concentration of 1-20mg/L for 20 hours to 24 hours; then the Diwu rhizomes are washed by running water for three or four times, put in a plastic box equipped with yellow sand substrate, and cultivated under the temperature ranging from 4 DEG C to 20 DEG C; finally the Diwu rhizomes are transplanted under artificial light or natural light. The method has an advantage that, the invention provides technical support to the improvement of Diwu biological yield, off-season culture and the conversion from wild to artificial.

Description

The method of relieving Diwu rhizome dormancy

Technical field

The invention belongs to agricultural plant culture technique field, be specifically related to a kind of method of relieving Diwu rhizome dormancy.

Background technology

The ground crow, the shady anemone (Anemone flaccida Fr.Schmidt) of formal name used at school woods, Ranunculaceae Anemone (Anemone L.), perennial herb, can be used as medicine after rhizome dries, mainly be distributed in each province, the middle and lower reach of Yangtze River, be grown in the dark and damp place of hillside sylvan life of height above sea level 1500～3000m.In Chinese Hubei province, emerge annual mid-March, and the death gradually of late May acrial part enters resting stage subsequently, and vegetative period is short, poor growth, and biological yield is little, and seminal propagation power is low, does not still have tame report at present.

Pharmaceutical research is found, the active ingredient of ground crow-triterpene saponin material, mainly contain clearing heat and detoxicating, the antalgic and sedative effect; Non-specific immune function and specific cellular immunity, humoral immunity all there is significant inhibitory effect.The anemone capsule of being made by Rhizoma Anemones flaccidae extract has the effect of wind-damp dispelling, strengthening the bones and muscles, swelling and pain relieving, can be used for treating various arthralgias and traumatic injury, and has no side effect.Because the ground crow grows slowly, yields poorly, and adds excavation blindly, wild resource can't satisfy the Chinese patent drug development requirement, and under field conditions (factors), every strain ground crow has only 1-2 blade, the black root-like stock rudiment in ground is few, and is general only by terminal bud germination growth accumulation biological yield.

Summary of the invention

The invention provides a kind of method of utilizing hormone and the effective relieving Diwu rhizome dormancy of temperature, provide technical support for improving black biomass in ground and off-season cultivation.

The present invention is achieved in that

In the early June-mid-November in every year, Mei Nian early November preferably, to be in the ground crow root-like stock of resting state, rinse well with running water, reject wherein rot and disease is arranged, soak behind the 20-24h (preferably with 1-20mg/L 6-benzyl aminopurine (6-BA), 6-BA concentration is 10-20mg/L), again with running water flushing 3-4 time, place in the plastic casing of yellow sand of the bacterium of going out (according to conventional sterilizing methods: 100 ℃ of high steams are sterilization 20h down), cover the gauze of preserving moisture above the plastic casing, place (8-16 ℃ of 4-20 ℃ day-night constant temperature or alternating temperature, use climatic cabinate, 16 ℃ of daytimes, 8 ℃ of evenings)) cultivate under the condition, keep the skin wet every day in right amount, the terminal bud lateral bud of processed rhizome of Rhizoma Anemones flaccidae is all sprouted after one week, they is transplanted in the culturing room of artificial lighting (intensity of illumination is about 2000lux) or natural lighting afterwards.

Compare with existing method, the present invention has the following advantages:

1, crow enters resting stage in June, and the present invention can effectively remove its dormancy by the processing of hormone and temperature, makes its anti-season be grown to serve as possibility.

2, adopt the present invention can make the black every strain number of blade in ground be increased to the 3-5 sheet, increased its photosynthetic area, effectively improved the annual biological yield of ground crow.

3, adopt the present invention can accelerate the sprouting speed of ground crow, overcome the shortcoming that black germination rate is low under the natural conditions, sprouting is slow.

Description of drawings

Fig. 1: be the sprouting situation after 20mg/L6-BA processing back is to the black breaking dormancy in resting state ground among one of them embodiment of the present invention;

Fig. 2: be the growing state after 20mg/L6-BA processing back is to the black breaking dormancy in resting state ground among one of them embodiment of the present invention.

Embodiment

Embodiment 1

Processing method: on June 8th, 2007 was to November 15, the root-like stock of crow wildly of Hubei governor sun county bronze drum bag will be picked up from, after under running water, rinsing well, after 6-benzyl aminopurine (6-BA) the immersion 24h of variable concentrations is set by table 1, running water flushing 3～4 times, place in the plastic casing in the yellow sand of high-temperature sterilization (according to conventional sterilizing methods, i.e. 100 ℃ of high steams sterilization 20h down), on cover the multilayer gauze and preserve moisture.Then, place 4 ℃ (day-night constant temperatures) as table 1 design, 8 ℃-16 ℃ (with 16 ℃ of climatic cabinate daytimes, 8 ℃ of evenings), 20 ℃ (day-night constant temperature) to sprout down respectively, keep the skin wet every day in right amount.The terminal bud lateral bud of processed rhizome of Rhizoma Anemones flaccidae is all sprouted after one week, they is transplanted in the culturing room of artificial lighting (intensity of illumination is about 2000lux) or natural lighting afterwards.12 rhizomes of every processing are treated to contrast with distilled water.Per 3 days black terminal bud germination numbers in investigation ground, sprouting of lateral bud number, terminal bud begin the to sprout diameter and the length of back 20 days investigation terminal buds, lateral bud.The invention effect sees Table 2-4.

The HORMONE TREATMENT concentration of table 1 relieving Diwu rhizome dormancy and treatment temperature design

The influence that the black over the ground root-like stock of table 2 different temperatures and HORMONE TREATMENT is sprouted

The size of terminal bud, lateral bud under table 3 different temperatures and the HORMONE TREATMENT

Indicate: terminal bud size=terminal bud diameter * terminal bud length lateral bud size=lateral bud diameter * lateral bud length

Table 2 is as can be known: under 4 ℃-20 ℃ temperature, the root-like stock that the 6-BA of variable concentrations handles all can be removed the terminal bud dormancy rapidly, and germination rate all reaches 100%.From terminal bud diameter and length, at 4 ℃, 8 ℃-16 ℃ (alternating temperatures), under 20 ℃, the optium concentration that 6-BA handles is to be 5mg/L, 20mg/L, 20mg/L successively; This shows that along with the rising of temperature, the optium concentration that adopts 6-BA to remove the root-like stock dormancy also is the trend of raising.

The terminal bud size is the most important index of the black trunk diameter growth in relation ground, and table 4 The results of analysis of variance shows that for the influence of terminal bud size, temperature, hormone and the effect of doing mutually of the two thereof all reach utmost point significance level.Wherein, under 8 ℃-16 ℃ (alternating temperatures) and 20 ℃, adopting the 6-BA of 20mg/L to handle 24h is the best approach that promotes the black terminal bud germination in ground, growth.

The action effect variance analysis of the black over the ground terminal bud size of table 4 temperature and 6-BA

Claims

1. the method for relieving Diwu rhizome dormancy, its step comprises cleans ground crow root-like stock, reject the rhizome that wherein rots, catches an illness, it is characterized in that, with the root-like stock of ground crow in annual early November, soak 20-24h with the 10-20mg/L 6-benzyl aminopurine after, again with running water flushing 3-4 time, place in the plastic casing of yellow sand of the bacterium of going out, cover the gauze of preserving moisture above the plastic casing; Place then under the 8-16 ℃ of condition and cultivated 7 days, keep the skin wet every day in right amount.