CN102465182A - 检测皮肤活性物质的检测试剂盒和检测皮肤活性物质的方法 - Google Patents

检测皮肤活性物质的检测试剂盒和检测皮肤活性物质的方法 Download PDF

Info

Publication number
CN102465182A
CN102465182A CN2011101423796A CN201110142379A CN102465182A CN 102465182 A CN102465182 A CN 102465182A CN 2011101423796 A CN2011101423796 A CN 2011101423796A CN 201110142379 A CN201110142379 A CN 201110142379A CN 102465182 A CN102465182 A CN 102465182A
Authority
CN
China
Prior art keywords
skin
klotho
active ingredients
detection
detection kit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2011101423796A
Other languages
English (en)
Inventor
崔向兑
孙义东
李真锳
罗勇柱
金汉坤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amorepacific Corp
Original Assignee
Amorepacific Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amorepacific Corp filed Critical Amorepacific Corp
Publication of CN102465182A publication Critical patent/CN102465182A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6881—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids from skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/148—Screening for cosmetic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Cosmetics (AREA)

Abstract

本发明涉及一种皮肤活性物质检测用试剂盒及利用该试剂盒检测皮肤活性物质的方法，具体地，涉及利用含有以长寿基因著称的klotho基因的检测试剂盒，从皮肤细胞中将诱导klotho基因表达的候选物质分离、精制，从而检测抗衰老及美白物质等皮肤活性物质的皮肤活性物质检测用试剂盒及利用该试剂盒检测皮肤活性物质的方法。

Description

检测皮肤活性物质的检测试剂盒和检测皮肤活性物质的方法
技术领域
本发明涉及一种皮肤活性物质检测用试剂盒及利用该试剂盒检测皮肤活性物质的方法，具体地，涉及利用含有以长寿基因著称的klotho基因的检测试剂盒，从皮肤细胞中将诱导klotho基因表达的候选物质分离、精制，从而检测抗衰老及美白物质等皮肤活性物质的皮肤活性物质检测用试剂盒及利用该试剂盒检测皮肤活性物质的方法。
背景技术
皮肤随着年龄的增长或者暴露在太阳光下而老化。随着老化，表皮的角质层脱落并更新所需要的时间延长，角质层增厚，真皮由于弹力纤维和保湿成分的含量下降或排列变化而产生皱纹。迄今为止，为了阻断引起皮肤衰老的原因中的一种光老化，人们对预防紫外线照射引起的皮肤变化进行了各种研究。另外，人们还研究了防止随着年龄增长的老化，即由遗传调节的老化的各种方法。这些研究都集中在寻找统筹调节老化的机制上。
对klotho基因的研究就是其中之一。敲除(knock-out)klotho基因的小鼠出现伴随1，25-(OH)2D3浓度上升出现动脉硬化、血管钙化、管组织钙化、肺气肿、活动减退、生殖腺形成异常、不孕、皮肤萎缩、运动失调症、低血糖症及高磷酸血症(Mutation of the mouse klotho gene leads to a syndromeresembling ageing.Nature 1997；390，45-51)。与此相反，klotho蛋白的表达增加时，出现寿命增加、胰岛素抗性增加及IGF-1抗性增加(Kurosu等，2005)。在人体中，也有报道称klotho蛋白的单核酸多态性与寿命减少、骨质疏松、中风及冠状动脉疾病有关(Arking等，2002；Kawano等，2002；Mullin等，2005；Ogata等，2002；Yamada等，2005)。
根据之前的研究，klotho主要在人的肾脏表达，在胎盘、前列腺及小肠中也有表达。但在klotho越来越重要的情况下，迄今为止未见klotho在皮肤细胞，即角质形成细胞、成纤维细胞或黑色素细胞中表达的报道。因此，也没有对增加klotho的表达的物质的研究的报道。
发明内容
发明目的
本发明的目的在于，制备一种能够在皮肤细胞中增加klotho蛋白表达的试剂盒，并利用该试剂盒测定在角质形成细胞、成纤维细胞及黑色素细胞等皮肤细胞中的klotho蛋白表达，发现能够检测抗老化物质及美白物质等皮肤活性物质，从而完成了本发明。
因此，本发明的目的在于提供一种通过在皮肤细胞中的klotho基因的表达与否，从而筛选皮肤活性物质的检测试剂盒及利用该试剂盒检测皮肤活性物质的方法。
技术方案
为了达到上述目的，本发明提供一种检测皮肤活性物质的检测试剂盒，该试剂盒含有klotho蛋白基因、用于扩增klotho蛋白基因的引物、探针和皮肤细胞，所述用于扩增klotho蛋白基因的引物具有SEQ ID No：1和SEQ IDNo：2所示的序列，所述探针具有SEQ ID No：3所示的序列，在所述皮肤细胞中能够筛选能够增加klotho蛋白表达量的物质，从而检测皮肤活性物质。
此外，本发明还提供利用所述检测试剂盒检测活性物质的方法。
有益效果
根据本发明的皮肤活性物质检测试剂盒，通过在皮肤细胞中的klotho基因的表达与否，可迅速简便地筛选如抗老化物质及美白物质等皮肤活性物质。
附图说明
图1a是在人胚胎肾脏细胞(HEK293)、角质形成细胞(NHK)及成纤维细胞(NHF)中测定klotho蛋白的RT-PCR扩增结果；图1b是在人黑色素细胞中测定klotho蛋白表达的RT-PCR扩增结果；此时，NHM-增强黑(NHM-promoblack)及NHM-DP是源自黑人的黑色素细胞，NHM-MP是源自亚洲人的黑色素细胞。
图2是在人胚胎肾脏细胞(HEK293)、角质形成细胞(NHK)及成纤维细胞(NHF)中检测klotho蛋白表达量的图。
图3是角质形成细胞经视黄酸(RA：retinoic acid)及视黄醇(ROL：retinol)处理后，在第2天及第8天检测klotho蛋白表达量的图。
具体实施方式
本发明涉及一种在皮肤细胞中检测klotho蛋白表达量的方法，通过此在皮肤细胞中筛选能够诱导长寿基因klotho蛋白表达的物质，从而检测皮肤活性物质。所述皮肤活性物质可包括改善皮肤皱纹或弹力或者改善粗糙皮肤的抗老化物质；及提亮肤色或改善由于痣、雀斑、皮肤杂质及太阳光线引起的皮肤晒黑等皮肤色素沉着现象的美白物质。
在皮肤细胞中能够检测klotho蛋白表达量的本发明的检测试剂盒含有klotho蛋白基因、用于扩增klotho蛋白的引物、探针(参照表1)和皮肤细胞，所述用于扩增klotho蛋白基因的引物具有SEQ ID No：1和SEQ ID No：2(序列号1和2)所示的序列，所述探针具有SEQ ID No：3(序列号3)所示的序列，在所述皮肤细胞中能够筛选能够增加klotho蛋白表达量的物质，从而检测抗老化物质。
表1
Figure BSA00000507874100041
根据本发明的一种实施方式，所述皮肤细胞可以是人角质形成细胞、成纤维细胞或黑色素细胞，但不限于此。
此外，本发明涉及利用所述检测试剂盒，将皮肤细胞用候选物质处理后，通过测定klotho蛋白表达与否的步骤及将能够增加klotho蛋白表达的物质分离及精制的步骤，从而检测皮肤活性物质的方法。
根据本发明的另一种实施方式，利用所述检测试剂盒，测定已知为抗氧化物质的视黄酸及视黄醇能够增加klotho蛋白表达，由此测定根据本发明的检测方法适合筛选未知的抗老化物质及皮肤活性物质。
以下，根据实施例及实验例详细说明本发明的内容。但这些实施例仅用于更好地理解本发明，本发明的权利范围并不由这些实施例和实验例限定，本领域技术人员应该理解的是本领域常用的各种变更、替换及插入都落入本发明的范围。
实验例1 在皮肤细胞中测定klotho蛋白表达与否
为了测定klotho蛋白是否在皮肤细胞中表达，制备了能够扩增klotho基因(NCBI记录号：NM_004795)的具有SEQ ID No：1和SEQ ID No：2所示的序列的引物，利用所述引物对和具有SEQ ID No：3所示的序列的探针(TaqManTM荧光探针)，通过RT-PCR观察在角质形成细胞(NHK)、成纤维细胞(NHF)，NHM-增强黑(NHM-promoblack)细胞(正常人表皮黑色素细胞(Normal Human Epidermal Melanocytes，NHEM)初生包皮，源自黑人的黑色素细胞，增强细胞(Promocell))，NHM-DP细胞(NHM-暗色素(NHM-darkpigment)；源自黑人的黑色素细胞，Invitrogen)和NHM-MP细胞(NHM-中等色素(NHM-moderate pigment)；源自亚洲人的黑色素细胞，Invitrogen)的cDNA中是否能够检出klotho的mRNA。此时，PCR反应条件为在94℃变性30秒，在55℃退火30秒，以及在72℃延伸60秒，反复上述循环35次。阳性对照组使用已知能够表达klotho基因的人胚胎肾脏细胞HEK293(humanembryonic kidney)细胞的cDNA，结果如图1a及图1b所示。从图1a及图1b的结果可看出，本发明中所使用的角质形成细胞、成纤维细胞及黑色素细胞中均有klotho基因表达。
此外，通过RT-PCR测定klotho mRNA在各细胞中的表达量的结果如图2所示。
实验例2在经视黄酸及视黄醇处理的角质形成细胞中klotho的mRNA表达量的测量
为了测定已知作为抗老化物质的视黄酸和视黄醇在角质形成细胞中对klotho表达的影响，在与所述实验例1相同的条件进行了RT-PCR，用所述视黄酸0.1及1μM和视黄醇1及10μM处理后，在第2天及第8天测量了klotho蛋白表达量，并将结果在图3中表示。
从图3的结果可以看出，用视黄酸和视黄醇处理后的第8天klotho蛋白的表达量显著增加。
Figure ISA00000507874300021

Claims (4)

1.一种检测皮肤活性物质的检测试剂盒，该试剂盒含有klotho蛋白基因、用于扩增klotho蛋白基因的引物、探针和皮肤细胞，所述用于扩增klotho蛋白基因的引物具有SEQ ID No：1和SEQ ID No：2所示的序列，所述探针具有SEQ ID No：3所示的序列，在所述皮肤细胞中能够筛选能够增加klotho蛋白表达量的物质，从而检测皮肤活性物质。
2.根据权利要求1所述的检测试剂盒，其中，所述皮肤活性物质是抗老化物质或者美白物质。
3.根据权利要求1所述的检测试剂盒，其中，所述皮肤细胞是人角质形成细胞、成纤维细胞或黑色素细胞。
4.一种检测皮肤活性物质的方法，其特征在于，该方法包括：利用权利要求1-3中任意一项所述的检测试剂盒，将皮肤细胞用候选物质处理后，测定klotho蛋白表达与否的步骤，和，将增加klotho蛋白表达的物质分离精制的步骤。
CN2011101423796A 2010-10-29 2011-05-27 检测皮肤活性物质的检测试剂盒和检测皮肤活性物质的方法 Pending CN102465182A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20100107000 2010-10-29
KR10-2010-0107000 2010-10-29

Publications (1)

Publication Number Publication Date
CN102465182A true CN102465182A (zh) 2012-05-23

Family

ID=46069301

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2011101423796A Pending CN102465182A (zh) 2010-10-29 2011-05-27 检测皮肤活性物质的检测试剂盒和检测皮肤活性物质的方法

Country Status (6)

Country Link
US (1) US20130252247A1 (zh)
EP (1) EP2634262B1 (zh)
JP (1) JP6186279B2 (zh)
KR (1) KR101907567B1 (zh)
CN (1) CN102465182A (zh)
WO (1) WO2012057567A2 (zh)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106868089A (zh) * 2015-12-11 2017-06-20 上海家化联合股份有限公司 一种利用皮肤成纤维细胞钾离子通道评价抗衰老功效的方法
WO2022121864A1 (zh) * 2020-12-08 2022-06-16 复旦大学 用于皮肤分型的标志物组合及其应用

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102012166B1 (ko) * 2012-11-20 2019-08-20 (주)아모레퍼시픽 피부 미백용 물질을 스크리닝하는 방법
KR102045247B1 (ko) * 2013-01-08 2019-11-15 (주)아모레퍼시픽 피부 면역세포를 이용하는 피부 활성물질을 탐색하는 방법 및 이를 이용한 키트
KR102017562B1 (ko) * 2013-03-13 2019-09-03 (주)아모레퍼시픽 미백 효능 물질의 스크리닝 방법
KR102053775B1 (ko) * 2013-07-23 2019-12-09 (주)아모레퍼시픽 미토콘드리아 역동성을 이용한 미백 물질 스크리닝 방법 및 이를 이용한 키트
KR20180032955A (ko) * 2016-09-23 2018-04-02 (주)아모레퍼시픽 Tnfrsf14 억제 물질을 포함하는 피부 미백용 조성물 및 tnfrsf14 억제 물질의 스크리닝 방법

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001020031A2 (en) * 1999-09-10 2001-03-22 Kyowa Hakko Kogyo Co Ltd Polymorphisms in a klotho gene
JP2003334088A (ja) * 2002-05-22 2003-11-25 Pharma Design Inc ヒト由来の新規Ｋｌｏｔｈｏ様タンパク質及びその遺伝子
JP2006158339A (ja) * 2004-12-09 2006-06-22 Kyoto Univ βＫｌｏｔｈｏ遺伝子、Ｃｙｐ７ａ１遺伝子、及びそれらの利用
CN1880480A (zh) * 2006-04-12 2006-12-20 重庆医科大学 检测等位基因特异引物－pcr方法及其在检测克老素基因多态性中的应用
CN101845482A (zh) * 2009-03-23 2010-09-29 上海芯超生物科技有限公司 一种女性皮肤美容基因检测芯片及其检测方法

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07112966B2 (ja) * 1986-03-26 1995-12-06 鐘紡株式会社 皮膚化粧料
AU2003211453A1 (en) * 2002-02-28 2003-09-09 Nihon Kefir Co., Ltd. External compositions for the skin
AU2003291884A1 (en) * 2002-12-10 2004-06-30 Ottawa Health Research Institute Modulation of stem cell differentiation by modulation of caspase-3 activity
KR100485326B1 (ko) * 2002-12-26 2005-04-27 주식회사 태평양 진세노사이드 화합물 ｋ로 이루어진 히알루론산 생성촉진제
JP2005089427A (ja) * 2003-09-22 2005-04-07 Kose Corp 皮膚外用剤
JP4541728B2 (ja) * 2004-03-09 2010-09-08 株式会社コーセー 皮膚外用剤
JP4587755B2 (ja) * 2004-09-17 2010-11-24 丸善製薬株式会社 抗炎症剤、美白剤、及び抗老化剤、並びに皮膚化粧料
JP2006290742A (ja) * 2005-04-05 2006-10-26 Kyoei Kagaku Kogyo Kk 老化防止化粧料
KR101318521B1 (ko) * 2005-08-23 2013-10-16 가오루 미야자키 피부 노화 마커와 그의 이용기술
JP5027528B2 (ja) * 2006-03-02 2012-09-19 株式会社ファンケル 皮膚老化の検査方法
US20090239936A1 (en) * 2006-05-15 2009-09-24 Yoshikazu Sugimoto Prophylactic and Therapeutic Agent for Cancer
JP5219344B2 (ja) * 2006-06-16 2013-06-26 三省製薬株式会社 皮膚外用剤
KR100835747B1 (ko) * 2006-08-25 2008-06-09 고려대학교 산학협력단 노화 관련 Ｍｒｅ11 단백질 및 이를 이용한 노화조절 방법
FR2908654B1 (fr) * 2006-11-20 2014-04-04 Oreal Utilisation cosmetique de proteines de type chitinase
JP4819727B2 (ja) * 2007-03-19 2011-11-24 株式会社コーセー 美白成分のスクリーニング方法
WO2008143145A1 (ja) * 2007-05-15 2008-11-27 Anbas Corporation 皮膚保湿作用の評価方法、およびこれを用いた保湿物質のスクリーニング方法
KR100840144B1 (ko) * 2007-05-17 2008-06-23 경상대학교산학협력단 미백 활성을 갖는 물질의 스크리닝 방법
FR2924613B1 (fr) * 2007-12-10 2012-12-21 Oreal Utilisation cosmetique de proteines de type hsp27.
FR2924947B1 (fr) * 2007-12-18 2010-03-19 Oreal Utilisation cosmetique de proteines de type calmodulin-like skin protein clsp
TW200936156A (en) * 2008-01-28 2009-09-01 Novartis Ag Methods and compositions using Klotho-FGF fusion polypeptides
US20090226901A1 (en) * 2008-03-06 2009-09-10 Kao Corporation Method of evaluating or selecting agents for preventing or improving pigmented spots
JP2010115131A (ja) * 2008-11-12 2010-05-27 Kao Corp 肌評価方法及び皮膚老化改善剤・皮膚老化予防剤のスクリーニング方法
WO2010065567A2 (en) * 2008-12-01 2010-06-10 Lifespan Extension Llc Methods and compositions for altering health, wellbeing, and lifespan
FR2940976B1 (fr) * 2009-01-13 2017-11-03 Oreal Utilisation a des fins de criblage d'actifs anti-ages de formes solubles de la proteine de type desmogleine i

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001020031A2 (en) * 1999-09-10 2001-03-22 Kyowa Hakko Kogyo Co Ltd Polymorphisms in a klotho gene
JP2003334088A (ja) * 2002-05-22 2003-11-25 Pharma Design Inc ヒト由来の新規Ｋｌｏｔｈｏ様タンパク質及びその遺伝子
JP2006158339A (ja) * 2004-12-09 2006-06-22 Kyoto Univ βＫｌｏｔｈｏ遺伝子、Ｃｙｐ７ａ１遺伝子、及びそれらの利用
CN1880480A (zh) * 2006-04-12 2006-12-20 重庆医科大学 检测等位基因特异引物－pcr方法及其在检测克老素基因多态性中的应用
CN101845482A (zh) * 2009-03-23 2010-09-29 上海芯超生物科技有限公司 一种女性皮肤美容基因检测芯片及其检测方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
"Establishment of a cell-based assay to screen regulators for Klotho gene promoter", 《ACTA PHARMACOLOGICA SINICA》 *
B.H.CHOI ET AL: "Transcriptional activation of the human Klotho gene by epidermal growth factor in HEK293 cells; role of Egr-1", 《GENE》 *
肖能明等: "Klotho is a serum factor related to human aging", 《CHINESE MEDICAL JOURNAL》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106868089A (zh) * 2015-12-11 2017-06-20 上海家化联合股份有限公司 一种利用皮肤成纤维细胞钾离子通道评价抗衰老功效的方法
CN106868089B (zh) * 2015-12-11 2020-02-28 上海家化联合股份有限公司 一种利用皮肤成纤维细胞钾离子通道评价抗衰老功效的方法
WO2022121864A1 (zh) * 2020-12-08 2022-06-16 复旦大学 用于皮肤分型的标志物组合及其应用

Also Published As

Publication number Publication date
EP2634262A9 (en) 2014-11-19
WO2012057567A2 (ko) 2012-05-03
EP2634262A2 (en) 2013-09-04
WO2012057567A3 (ko) 2012-09-07
EP2634262B1 (en) 2017-05-31
KR101907567B1 (ko) 2018-10-15
JP2013541960A (ja) 2013-11-21
US20130252247A1 (en) 2013-09-26
KR20120049126A (ko) 2012-05-16
JP6186279B2 (ja) 2017-08-23
EP2634262A4 (en) 2014-04-16

Similar Documents

Publication Publication Date Title
CN102465182A (zh) 检测皮肤活性物质的检测试剂盒和检测皮肤活性物质的方法
Jiang et al. Anti-senescence effect and molecular mechanism of the major royal jelly proteins on human embryonic lung fibroblast (HFL-I) cell line
US11913061B2 (en) Method for preparing nucleic acid sample
Slominski et al. Expression of hypothalamic–pituitary–thyroid Axis RelatedGenes in the human skin
EP2647724B1 (en) Method for screening a substance for preventing skin aging
JP2017070306A (ja) 色素沈着過剰状態の遺伝子発現シグネチャーを生成する方法
EP2704728A1 (en) Expression signatures of genes and gene networks associated with skin aging
CN103619318B (zh) 和细胞外基质相关的皮肤色斑分子标签
JP2007259851A (ja) 皮膚老化の検査方法
Strömberg et al. Transcriptional profiling of melanocytes from patients with vitiligo vulgaris
Lena et al. Skn-1a/Oct-11 and ΔNp63α exert antagonizing effects on human keratin expression
TPCN Development of lentigines in German and Japanese women correlates with variants in the SLC45A2 gene
Roumans et al. Variation in extracellular matrix genes is associated with weight regain after weight loss in a sex-specific manner
CN114340677B (zh) Jwa基因以及相关化合物的抗衰老用途
US20130324476A1 (en) Cosmetic use of dermicidin, and analogues or fragments thereof
Oh et al. The effects of uncoupling protein-1 genotype on lipoprotein cholesterol level in Korean obese subjects
Mandal et al. Genetics of colour variation in wild versus cultured queen loach, Botia dario (Hamilton, 1822)
Lü et al. High-throughput integrated analyses for the tyrosinase-induced melanogenesis: microarray, proteomics and interactomics studies
Pavicic et al. Correlation analysis between mtDNA 4977-bp deletion and ageing
EP2349319B1 (en) Use of proteins of dj-1 type for characterizing skin dryness
EP2975410B1 (en) Method for screening substance having whitening efficacy
JP5275613B2 (ja) 皮膚のシミ形成を予測する皮膚検査方法
JP7437732B2 (ja) 表皮ターンオーバー遅延の遺伝的素因の判定方法
US20210310072A1 (en) Method for screening for skin whitening agent by using sdf1 promoter region
Han et al. The role of mitochondrial DNA mutations in a Han Chinese population on sepsis pathogenesis.

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20120523

RJ01 Rejection of invention patent application after publication