CN102458665A - 用于从生物学样品中分离靶生物实体的方法和仪器 - Google Patents

用于从生物学样品中分离靶生物实体的方法和仪器 Download PDF

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Publication number
CN102458665A
CN102458665A CN2010800262983A CN201080026298A CN102458665A CN 102458665 A CN102458665 A CN 102458665A CN 2010800262983 A CN2010800262983 A CN 2010800262983A CN 201080026298 A CN201080026298 A CN 201080026298A CN 102458665 A CN102458665 A CN 102458665A
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China
Prior art keywords
magnetic
flow path
fluid flow
module
separation module
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CN2010800262983A
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English (en)
Chinese (zh)
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H·M·钱德勒
M·B·钱德勒
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Clinical Genomics Pty Ltd
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Clinical Genomics Pty Ltd
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Publication of CN102458665A publication Critical patent/CN102458665A/zh
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B03SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03CMAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00Magnetic separation
    • B03C1/02Magnetic separation acting directly on the substance being separated
    • B03C1/28Magnetic plugs and dipsticks
    • B03C1/288Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B03SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03CMAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00Magnetic separation
    • B03C1/005Pretreatment specially adapted for magnetic separation
    • B03C1/01Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326Magnetic particles
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B03SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03CMAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00Details of magnetic or electrostatic separation
    • B03C2201/18Magnetic separation whereby the particles are suspended in a liquid
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B03SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03CMAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00Details of magnetic or electrostatic separation
    • B03C2201/26Details of magnetic or electrostatic separation for use in medical or biological applications

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN2010800262983A 2009-04-22 2010-04-22 用于从生物学样品中分离靶生物实体的方法和仪器 Pending CN102458665A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US17153209P 2009-04-22 2009-04-22
US61/171,532 2009-04-22
US18266109P 2009-05-29 2009-05-29
US61/182,661 2009-05-29
US28528609P 2009-12-10 2009-12-10
US61/285,286 2009-12-10
PCT/AU2010/000459 WO2010121315A1 (en) 2009-04-22 2010-04-22 Method and apparatus for isolating a target bioentity from a biological sample

Publications (1)

Publication Number Publication Date
CN102458665A true CN102458665A (zh) 2012-05-16

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN2010800262983A Pending CN102458665A (zh) 2009-04-22 2010-04-22 用于从生物学样品中分离靶生物实体的方法和仪器

Country Status (9)

Country Link
US (1) US20120115167A1 (https=)
EP (1) EP2421654A1 (https=)
JP (1) JP2012524885A (https=)
CN (1) CN102458665A (https=)
AU (1) AU2010239154A1 (https=)
IL (1) IL215807A0 (https=)
NZ (1) NZ595982A (https=)
RU (1) RU2011147219A (https=)
WO (1) WO2010121315A1 (https=)

Cited By (5)

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CN102911864A (zh) * 2012-10-15 2013-02-06 宁波美晶医疗技术有限公司 一种血液中稀有细胞的分离仪
CN103131693A (zh) * 2013-02-25 2013-06-05 宁波美晶医疗技术有限公司 一种血液中稀有细胞的分离方法
CN103131634A (zh) * 2013-02-25 2013-06-05 宁波美晶医疗技术有限公司 一种基于微磁场的免疫肿瘤细胞筛选系统
CN103940997A (zh) * 2014-03-21 2014-07-23 上海柏慧康生物科技有限公司 一种乳腺癌循环肿瘤细胞检测系统及试剂盒
CN115655825A (zh) * 2015-09-18 2023-01-31 紫荆实验室股份有限公司 利用表面附着结构的流动池,以及相关的系统和方法

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HRP20171468T1 (hr) * 2010-11-09 2017-12-15 Arcedi Biotech Aps Obogaćivanje i identificiranje fetalnih stanica u majčinskoj krvi, te ligandi namijenjenu u tu svrhu
WO2012113028A1 (en) * 2011-02-23 2012-08-30 Clinical Genomics Pty. Ltd. Isolation of a target bioentity from a biological sample
US20140295420A1 (en) * 2011-06-15 2014-10-02 Koninklijke Philips N.V. Processing of biological sample components
WO2013003898A1 (en) * 2011-07-07 2013-01-10 Clinical Genomics Pty. Ltd Method for detection of cancer in a patient
DE102011088741B4 (de) 2011-12-15 2013-07-25 Institut für Bioprozess- und Analysenmesstechnik e.V. Verfahren und Anordnung zum Markieren und Separieren von Zellen aus einer Zellsuspension
WO2013123035A1 (en) 2012-02-13 2013-08-22 Molecular Systems Corporation System and method for processing and detecting nucleic acids
US9604213B2 (en) 2012-02-13 2017-03-28 Neumodx Molecular, Inc. System and method for processing and detecting nucleic acids
US11648561B2 (en) 2012-02-13 2023-05-16 Neumodx Molecular, Inc. System and method for processing and detecting nucleic acids
US9637775B2 (en) 2012-02-13 2017-05-02 Neumodx Molecular, Inc. System and method for processing biological samples
US20140024136A1 (en) * 2012-07-19 2014-01-23 Derek L. Chappell Magnetic particle separator
US20140120544A1 (en) 2012-10-25 2014-05-01 Neumodx Molecular, Inc. Method and materials for isolation of nucleic acid materials
EP3058316B1 (en) * 2013-10-18 2019-03-13 The General Hospital Corporation Microfluidic sorting using high gradient magnetic fields
US10690662B2 (en) 2014-11-28 2020-06-23 Indian Institute Of Technology Delhi Magnetic capturing of rare cells
EP3061529A1 (en) * 2015-02-24 2016-08-31 AdnaGen GmbH Apparatus and method for the analysis, isolation and/or enrichment of target structures in a fluid sample
WO2016183032A1 (en) 2015-05-08 2016-11-17 Biomagnetic Solutions Llc Apparatus and method for immunomagnetic cell separation
WO2017053630A1 (en) * 2015-09-22 2017-03-30 Purdue Research Foundation Centrifuge-free isolation and detection of rare cells
EP3735281B1 (en) 2018-01-05 2024-11-27 Path Ex, Inc. Device for the capture and removal of disease material from fluids
JP7396777B2 (ja) * 2018-09-28 2023-12-12 オクタン バイオテック インコーポレーテッド 磁気分離
EP3870969A4 (en) * 2018-10-24 2022-07-27 Path Ex, Inc. PROCESS FOR RECOVERING AND ISOLATION OF DISEASE MATERIAL FROM FLOWING MATERIAL
IL308999B2 (en) 2019-06-07 2026-02-01 Arcedi Biotech Aps Isolation of fetal cells using FACS
JP2022545485A (ja) * 2019-08-30 2022-10-27 フロデザイン ソニックス, インク. 複数の標的受容体のための音響親和性細胞の選択
CN113125707A (zh) * 2020-01-10 2021-07-16 陈琪 一种分离磁微粒的方法、装置及设备
EP4616195A1 (en) * 2022-11-08 2025-09-17 Zhong Li Methods for detecting target compounds and uses thereof
CN120838569B (zh) * 2025-07-30 2026-01-06 上海迪化科技股份有限公司 一种磁珠连续分离工艺及装置
CN120644311B (zh) * 2025-07-30 2026-02-06 上海迪化科技股份有限公司 一种磁珠连续分离系统和控制方法

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CN1899698A (zh) * 2006-06-30 2007-01-24 上海师范大学 磁性分离柱及其在生物样品分离中的应用
WO2009026566A1 (en) * 2007-08-23 2009-02-26 Cynvenio Biosystems, Llc Trapping magnetic sorting system for target species

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US5795470A (en) * 1991-03-25 1998-08-18 Immunivest Corporation Magnetic separation apparatus
CN1506460A (zh) * 2002-12-09 2004-06-23 �廪��ѧ 从样品中分离细胞粒子的方法及其试剂盒
CN1899698A (zh) * 2006-06-30 2007-01-24 上海师范大学 磁性分离柱及其在生物样品分离中的应用
WO2009026566A1 (en) * 2007-08-23 2009-02-26 Cynvenio Biosystems, Llc Trapping magnetic sorting system for target species

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102911864A (zh) * 2012-10-15 2013-02-06 宁波美晶医疗技术有限公司 一种血液中稀有细胞的分离仪
CN103131693A (zh) * 2013-02-25 2013-06-05 宁波美晶医疗技术有限公司 一种血液中稀有细胞的分离方法
CN103131634A (zh) * 2013-02-25 2013-06-05 宁波美晶医疗技术有限公司 一种基于微磁场的免疫肿瘤细胞筛选系统
CN103940997A (zh) * 2014-03-21 2014-07-23 上海柏慧康生物科技有限公司 一种乳腺癌循环肿瘤细胞检测系统及试剂盒
CN115655825A (zh) * 2015-09-18 2023-01-31 紫荆实验室股份有限公司 利用表面附着结构的流动池,以及相关的系统和方法

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Publication number Publication date
EP2421654A1 (en) 2012-02-29
AU2010239154A1 (en) 2011-11-10
RU2011147219A (ru) 2013-05-27
US20120115167A1 (en) 2012-05-10
WO2010121315A1 (en) 2010-10-28
NZ595982A (en) 2013-10-25
JP2012524885A (ja) 2012-10-18
IL215807A0 (en) 2012-01-31

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Application publication date: 20120516