CN102439444B - 测量g蛋白偶联受体激活 - Google Patents

测量g蛋白偶联受体激活 Download PDF

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Publication number
CN102439444B
CN102439444B CN201080014630.4A CN201080014630A CN102439444B CN 102439444 B CN102439444 B CN 102439444B CN 201080014630 A CN201080014630 A CN 201080014630A CN 102439444 B CN102439444 B CN 102439444B
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protein
subunit
coupled receptor
cell
bioluminescent
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CN102439444A (zh
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S·C·特罗韦尔
I·M·霍恩
H·戴克斯
V·利奇
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Commonwealth Scientific and Industrial Research Organization CSIRO
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Mycology (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201080014630.4A 2009-01-29 2010-01-28 测量g蛋白偶联受体激活 Active CN102439444B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14827109P 2009-01-29 2009-01-29
US61/148,271 2009-01-29
PCT/AU2010/000080 WO2010085844A1 (en) 2009-01-29 2010-01-28 Measuring g protein coupled receptor activation

Publications (2)

Publication Number Publication Date
CN102439444A CN102439444A (zh) 2012-05-02
CN102439444B true CN102439444B (zh) 2014-10-22

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US (1) US8647887B2 (cg-RX-API-DMAC7.html)
EP (1) EP2382470B1 (cg-RX-API-DMAC7.html)
JP (1) JP5684145B2 (cg-RX-API-DMAC7.html)
KR (1) KR101834574B1 (cg-RX-API-DMAC7.html)
CN (1) CN102439444B (cg-RX-API-DMAC7.html)
AU (1) AU2010207880B2 (cg-RX-API-DMAC7.html)
CA (1) CA2748500C (cg-RX-API-DMAC7.html)
IL (1) IL213771A0 (cg-RX-API-DMAC7.html)
NZ (1) NZ593294A (cg-RX-API-DMAC7.html)
SG (1) SG172321A1 (cg-RX-API-DMAC7.html)
WO (1) WO2010085844A1 (cg-RX-API-DMAC7.html)

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CN103149111A (zh) * 2013-02-26 2013-06-12 浙江大学 一种基于嗅觉受体传感器检测气味物质丁二酮的方法
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WO2016058094A1 (en) * 2014-10-14 2016-04-21 Université de Montréal BIOSENSOR BASED ON GβΥ-INTERACTING PROTEINS TO MONITOR G-PROTEIN ACTIVATION
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CN105018564B (zh) * 2015-07-22 2017-03-08 华东师范大学 一种g蛋白‑偶联受体活体示踪方法及应用
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CN105671119A (zh) * 2016-03-02 2016-06-15 昆明理工大学 一种基于组胺受体3分子探针的药物活性成分筛选方法
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JP7215999B2 (ja) * 2016-12-21 2023-01-31 エフ．ホフマン－ラ ロシュ アーゲー 抗体またはリガンドの結合および機能を決定するためのアッセイ法
US20190342356A1 (en) * 2017-01-02 2019-11-07 Koninklijke Kpn N.V. Selectively updating a dynamic manifest file
CN106872435B (zh) * 2017-04-14 2019-07-16 重庆大学 基于苝酰亚胺多臂聚合物的蛋白质生物发光成像传感器
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CN113501881B (zh) * 2017-09-27 2023-07-28 北京大学 融合蛋白
US20190194573A1 (en) * 2017-12-21 2019-06-27 Aromyx Corporation Scent, Smell and Odor Creation from an Aromagraph
CN108586620B (zh) * 2018-05-10 2021-11-23 山东大学 筛选昆虫性信息素受体的配体的传感器分子以及筛选方法
US11078200B2 (en) 2018-06-29 2021-08-03 International Paper Company Synthesis of coelenterazine
US11008326B2 (en) 2018-06-29 2021-05-18 International Paper Company Synthesis of coelenterazine
CN110252079B (zh) * 2019-05-08 2022-03-15 天津大学 一种气味远程传输的方法
KR102264116B1 (ko) * 2019-06-19 2021-06-14 한국과학기술연구원 세포 소포체 내 g-단백질 연결 수용체(gpcr)의 실시간 활성 상태 분석 방법 및 컴퓨터 프로그램
CN110577593B (zh) * 2019-07-01 2021-09-14 华中农业大学 一种小分子近红外光荧光蛋白及其融合蛋白
CN115362263A (zh) * 2020-02-11 2022-11-18 Absci公司 邻近测定
DE102023204533B4 (de) * 2023-05-15 2025-03-27 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung eingetragener Verein Verfahren für die Durchführung von biochemischen Untersuchungen an Proben
CN118501118B (zh) * 2024-07-16 2024-10-29 汉王科技股份有限公司 一种基于细胞的生物荧光能量共振转移检测系统及应用
CN119409840B (zh) * 2025-01-06 2025-07-01 北京大学 基于g蛋白偶联受体构建的比率型荧光探针及其构建方法

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