Summary of the invention
Technical problem to be solved by this invention is to overcome the deficiencies in the prior art, and a kind of biological organic fertilizer that is applied to fruit tree is provided.
Technical problem to be solved by this invention realizes by following technological approaches;
Be applied to a biological organic fertilizer for fruit tree, by each component of following weight part, formed:
Bacillus subtilis microbial agent (Bacillus subtilis) 10-30 part, yeast saccharomyces cerevisiae bacteria agent (Saccharomyces cerevisiae) 8-12 part, plant lactobacillus microbial inoculum (Lactobacillusplantarum) 8-12 part, Jingyang drappus microbial inoculum (Streptomyces jingyangensis) 10-30 part and dregs of beans 50-70 part.
Preferably, the weight part of each component is: 20 parts of bacillus subtilis microbial agents, 10 parts of yeast saccharomyces cerevisiae bacteria agents, 10 parts of plant lactobacillus microbial inoculums, 20 parts of Jingyang drappus microbial inoculums, 60 parts of dregs of beans.
Microbial inoculum described in the present invention is preferably powdery microbial inoculum.
In the present invention, subtilis used (microbial inoculum), S. cervisiae (microbial inoculum), plant lactobacillus (microbial inoculum), Jingyang streptomycete (microbial inoculum) can be bought from commercial channels and obtain described bacterial classification, for example: DSMZ of Institute of Microorganism, Academia Sinica (network address http://www.cgmcc.net), the culture medium prescription providing according to supplier again and culture temperature, just can prepare corresponding microbial inoculum according to conventional fermentation process.
Dregs of beans is a kind of byproduct obtaining after soybean extracting bean oil, and the dregs of beans described in the present invention can be bought and obtain from market, and its specification meets national sector standard.
Another technical problem to be solved by this invention is to provide a kind of method of preparing above-mentioned biological organic fertilizer.
Another technical problem to be solved by this invention is achieved through the following technical solutions:
A method of preparing described biological organic fertilizer, comprising:
(1) by described weight part, take each component: bacillus subtilis microbial agent 10-30 part, yeast saccharomyces cerevisiae bacteria agent 8-12 part, plant lactobacillus microbial inoculum 8-12 part, Jingyang drappus microbial inoculum 10-30 part and dregs of beans 50-70 part;
(2) will after dregs of beans pulverizing, mix, be dried, pack and get final product with 4 kinds of microbial inoculums.
In every gram of fertilizer of biological organic fertilizer of the present invention, the effective strain number of contained microbial inoculum is more than or equal to 0.20 hundred million/g.
The using method of biological organic fertilizer of the present invention:
Live: as base manure spread fertilizer over the fields, ditch spread all can, 8-16 kilogram is used on every mu of ground, recommends 12 kilograms of consumptions.
Field planting: use 4-6 kilogram for every mu when fruit tree is transplanted, every mu of best fruiting period use 12-16 kilogram.
Biological organic fertilizer of the present invention mainly has the effect of following several respects:
1, during biological organic fertilizer of the present invention is manured into soil beneficial microorganism, can effectively supplement and adjust soil ecology flora, decompose, change, synthesize available nutrient, improve the utilization ratio of fertilizer;
2, suppress various soil-borne diseases, repair and harden and ill soil, promote fruit tree growth;
3, improve fruit tree immunologic function, suppress various common and multiple diseases, reduce pesticide dosage, reduce production costs;
4, improve fruit tree resistance, particularly combating a natural disaster under the extreme climate conditions such as low temperature, arid, mitigation ability.
5, the fruit-setting rate that improves fruit tree, improves fruit quality, and development green non-pollution agricultural is had to positive meaning.
Embodiment
Below in conjunction with specific embodiment, further describe the present invention, advantage and disadvantage of the present invention will be more clear along with description.But these embodiment are only exemplary, scope of the present invention are not formed to any restriction.It will be understood by those skilled in the art that lower without departing from the spirit and scope of the present invention and can the details of technical solution of the present invention and form be modified or be replaced, but these modifications and replacement all fall within the scope of protection of the present invention.
Bacillus subtilis strain, S. cervisiae bacterial classification, plant lactobacillus bacterial classification are all purchased from DSMZ of Institute of Microorganism, Academia Sinica, and its numbering is respectively: subtilis CGMCC1.1630, S. cervisiae CGMCC2.2081, plant lactobacillus CGMCC1.124; Jingyang streptomyces species is purchased from microbial strains preservation center, Liaoning Province, bacterial strain deposit number: LICC.40041.
The preparation of preparation embodiment 1 bacillus subtilis microbial agent
Subtilis (Bacillus subtilis) (DSMZ of Institute of Microorganism, Academia Sinica, goods number: the CGMCC1.1630) preparation of microbial inoculum:
1, Bacillus subtilis strain is inoculated in substratum (the formulated substratum providing by supplier) and cultivates, will in 4 ℃ of refrigerators of medium slant seed bacterial strain, store, before using, take out twice of activation.
2, the bacterial strain after activation is turned to 250 milliliters of eggplant bottle nutrient agar mediums and cultivate, culture temperature is 37 ℃, washes down, as the inoculum of seeding tank during 48 hours well-growns with the water bacterium of going out.
3, in the fermentor tank of 1 ton, add substratum, it consists of: Semen Maydis powder 3%, soybean cake powder 3%, bran powder 3%, K
2hPO
42.15%, KH
2pO
40.8%, MgSO
40.075%, peanut oil 0.3%; Initial pH7.5.From seeding tank to producing tank inoculation, start fermentation.
4, fermentation condition: culture temperature is 37 ℃, ventilation is 1: 1.1, tank pressure is 0.05mpa/cm
2stirring velocity is 220rpm, inoculate after 20 hours and sampled once every 4 hours, observe thalli growth situation, whether have pollution, after finding that spore generation reaches more than 90%, liquid fermenting completes, and in zymocyte liquid, adds conventional sorbent material and weighting agent xanthan gum, obtains bacillus subtilis microbial agent.
The preparation of preparation embodiment 2 yeast saccharomyces cerevisiae bacteria agents, plant lactobacillus microbial inoculum and Jingyang drappus microbial inoculum
The culture medium prescription providing according to supplier and culture temperature, prepare respectively yeast saccharomyces cerevisiae bacteria agent, plant lactobacillus microbial inoculum and Jingyang drappus microbial inoculum according to the conventional fermentation process preparation method of the bacillus subtilis microbial agent described in embodiment 1 (can with reference to preparation).
The preparation of embodiment 1 biological organic fertilizer
By following weight, take each component: dregs of beans 60kg, bacillus subtilis microbial agent 20kg, yeast saccharomyces cerevisiae bacteria agent 10kg, plant lactobacillus microbial inoculum 10kg and Jingyang drappus microbial inoculum 20kg.
After dregs of beans is pulverized, mix, be dried, pack and get final product with above-mentioned microbial inoculum.
The preparation of embodiment 2 biological organic fertilizers
By following weight, take each component: dregs of beans 50kg, bacillus subtilis microbial agent 30kg, yeast saccharomyces cerevisiae bacteria agent 12kg, plant lactobacillus microbial inoculum 8kg, Jingyang drappus microbial inoculum 30kg;
After dregs of beans is pulverized, mix, be dried, pack and get final product with above-mentioned microbial inoculum.
The preparation of embodiment 3 biological organic fertilizers
By following weight, take each component: dregs of beans 70kg, bacillus subtilis microbial agent 14kg, yeast saccharomyces cerevisiae bacteria agent 8kg, plant lactobacillus microbial inoculum 8kg and Jingyang drappus microbial inoculum 14kg.
After dregs of beans is pulverized, mix, be dried, pack and get final product with above-mentioned three kinds of microbial inoculums.
The preparation of embodiment 4 biological organic fertilizers
By following weight, take each component: dregs of beans 70kg, bacillus subtilis microbial agent 10kg, yeast saccharomyces cerevisiae bacteria agent 8kg, plant lactobacillus microbial inoculum 8kg and Jingyang drappus microbial inoculum 10kg.
After dregs of beans is pulverized, mix, be dried, pack and get final product with above-mentioned three kinds of microbial inoculums.
The preparation of embodiment 5 biological organic fertilizers
By following weight, take each component: dregs of beans 65kg, bacillus subtilis microbial agent 20kg, yeast saccharomyces cerevisiae bacteria agent 10kg, plant lactobacillus microbial inoculum 9kg and Jingyang drappus microbial inoculum 18kg.
After dregs of beans is pulverized, mix, be dried, pack and get final product with above-mentioned three kinds of microbial inoculums.
The preparation of embodiment 6 biological organic fertilizers
By following weight, take each component: dregs of beans 55kg, bacillus subtilis microbial agent 25kg, yeast saccharomyces cerevisiae bacteria agent 9kg, plant lactobacillus microbial inoculum 11kg and Jingyang drappus microbial inoculum 25kg.
After dregs of beans is pulverized, mix, be dried, pack and get final product with above-mentioned three kinds of microbial inoculums.The effect test of test example 1 biological organic fertilizer of the present invention on the pear tree of Daxing
One, test materials
For the prepared biological organic fertilizer of examination fertilizer: embodiment of the present invention 1-6;
Trial crops: pear tree (kind: red bar pears, every mu of 83 strains of planting density).
Two, test site and time
Test site: Dong Zhao village, high official position shop town, Daxing District;
Test period: year September in October, 2009 to 2010;
Three, test method
(1) test is made as treatment group and control group, 1 mu of the community area of each treatment group or control group;
Control group: conventional fertilizer application.
Treatment group: for examination fertilizer (for examination fertilizer 12kg/ mu)+conventional fertilizer application.
Wherein, process element is 6 treatment group:
Process 1 group: biological organic fertilizer (12kg/ the mu)+conventional fertilizer application that embodiment 1 is prepared;
Process 2 groups: biological organic fertilizer (12kg/ the mu)+conventional fertilizer application that embodiment 2 is prepared;
Process 3 groups: biological organic fertilizer (12kg/ the mu)+conventional fertilizer application that embodiment 3 is prepared;
Process 4 groups: biological organic fertilizer (12kg/ the mu)+conventional fertilizer application that embodiment 4 is prepared;
Process 5 groups: biological organic fertilizer (12kg/ the mu)+conventional fertilizer application that embodiment 5 is prepared;
Process 6 groups: biological organic fertilizer (12kg/ the mu)+conventional fertilizer application that embodiment 6 is prepared;
6 treatment group and control group at aspects such as soil regime, fruit tree growing ways without significant difference.
(2) soil nutrient status experimental field
Table 1
(3) fertilising basic condition
Control group: conventional fertilizer application, base manure is primary ammonium phosphate 15kg/ mu, urea 15kg/ mu.
6 treatment group: use respectively the mu for examination fertilizer 12kg/ for every mu, all the other fertilisings and field management etc. are identical with control group.
(4) measure content
Mensuration project: output, fruit weight.
Four, results and analysis
(1) impact of different treatment on the output of pears
Table 2
Group |
Output (kg/ mu) |
Than control group volume increase (kg/ mu) |
Compare the stimulation ratio (%) of control group |
Control group |
464.8 |
-- |
-- |
Process 1 group |
556.5 |
91.7 |
19.7 |
Process 2 groups |
523.2 |
58.4 |
12.6 |
Process 3 groups |
519.4 |
54.6 |
11.7 |
Process 4 groups |
514.6 |
49.8 |
10.7 |
Process 5 groups |
509.7 |
44.9 |
9.7 |
Process 6 groups |
513.7 |
48.9 |
10.5 |
(2) impact of different treatment on the fruit weight of pears
Fruit weight measuring method: 30 of representational fruits are chosen in each processing, measures fruit weight, calculating mean value.
Table 3
Group |
Fruit weight (g/) |
Than control group, increase (g/) |
Compare the increment rate (%) of control group |
Control group |
206.0 |
-- |
-- |
Process 1 group |
251.2 |
45.2 |
21.9 |
Process 2 groups |
236.9 |
25.9 |
15.0 |
Process 3 groups |
227.8 |
21.8 |
10.6 |
Process 4 groups |
229.9 |
23.9 |
11.6 |
Process 5 groups |
225.8 |
19.8 |
9.6 |
Process 6 groups |
226.9 |
20.9 |
10.1 |
Five, conclusion (of pressure testing)
From the testing data of table 2 and table 3, use biological organic fertilizer of the present invention and can effectively improve edatope, improve chemical fertilizer utilization ratio, significantly improve output of the fruit tree, increase single fruit weight and improve fruit quality; Wherein, the prepared biological organic fertilizer of embodiment 1 is improving output of the fruit tree and is increasing in the effect of single fruit weight more remarkable than other biological organic fertilizer.