CN102427738B - 用于保存与用于口服再水化溶液的粉剂混合的冻干的乳酸菌的产品 - Google Patents
用于保存与用于口服再水化溶液的粉剂混合的冻干的乳酸菌的产品 Download PDFInfo
- Publication number
- CN102427738B CN102427738B CN201080021605.9A CN201080021605A CN102427738B CN 102427738 B CN102427738 B CN 102427738B CN 201080021605 A CN201080021605 A CN 201080021605A CN 102427738 B CN102427738 B CN 102427738B
- Authority
- CN
- China
- Prior art keywords
- anhydrous
- freeze
- oral rehydration
- lactic acid
- product
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title claims abstract description 46
- 241000894006 Bacteria Species 0.000 title claims abstract description 27
- 239000000310 rehydration solution Substances 0.000 title claims abstract description 24
- 239000004310 lactic acid Substances 0.000 title claims abstract description 23
- 235000014655 lactic acid Nutrition 0.000 title claims abstract description 23
- 239000000843 powder Substances 0.000 title abstract description 10
- 238000003860 storage Methods 0.000 title description 9
- 238000004806 packaging method and process Methods 0.000 claims abstract description 33
- 239000000463 material Substances 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 14
- 239000011888 foil Substances 0.000 claims abstract description 11
- 241000186604 Lactobacillus reuteri Species 0.000 claims description 21
- 229940001882 lactobacillus reuteri Drugs 0.000 claims description 20
- 238000004108 freeze drying Methods 0.000 claims description 17
- 239000006041 probiotic Substances 0.000 claims description 13
- 230000000529 probiotic effect Effects 0.000 claims description 13
- 235000018291 probiotics Nutrition 0.000 claims description 13
- 238000004321 preservation Methods 0.000 claims description 11
- 239000004698 Polyethylene Substances 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 8
- 239000005030 aluminium foil Substances 0.000 claims description 8
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 8
- -1 polyethylene Polymers 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 6
- 229920000573 polyethylene Polymers 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 4
- 229910052782 aluminium Inorganic materials 0.000 claims description 4
- 229960004543 anhydrous citric acid Drugs 0.000 claims description 4
- 239000001103 potassium chloride Substances 0.000 claims description 4
- 235000011164 potassium chloride Nutrition 0.000 claims description 4
- 239000011734 sodium Substances 0.000 claims description 4
- 229910052708 sodium Inorganic materials 0.000 claims description 4
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 4
- 229960001763 zinc sulfate Drugs 0.000 claims description 4
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 4
- 239000004411 aluminium Substances 0.000 claims description 3
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 claims description 3
- 239000000292 calcium oxide Substances 0.000 claims description 3
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 3
- 235000011194 food seasoning agent Nutrition 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 2
- 230000007774 longterm Effects 0.000 claims 1
- 239000002274 desiccant Substances 0.000 abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 17
- 150000003839 salts Chemical class 0.000 description 16
- 244000005700 microbiome Species 0.000 description 10
- 206010012735 Diarrhoea Diseases 0.000 description 8
- 230000036571 hydration Effects 0.000 description 7
- 238000006703 hydration reaction Methods 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- PNEYBMLMFCGWSK-UHFFFAOYSA-N Alumina Chemical compound [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 230000035899 viability Effects 0.000 description 5
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 4
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 230000010354 integration Effects 0.000 description 4
- 238000012856 packing Methods 0.000 description 4
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 3
- 241000186660 Lactobacillus Species 0.000 description 3
- 229940071604 biogaia Drugs 0.000 description 3
- 239000001110 calcium chloride Substances 0.000 description 3
- 229910001628 calcium chloride Inorganic materials 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 229940039696 lactobacillus Drugs 0.000 description 3
- 239000010410 layer Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000005022 packaging material Substances 0.000 description 3
- 239000012047 saturated solution Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 2
- 241000186000 Bifidobacterium Species 0.000 description 2
- 241000917009 Lactobacillus rhamnosus GG Species 0.000 description 2
- CPLXHLVBOLITMK-UHFFFAOYSA-N Magnesium oxide Chemical compound [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 description 2
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 235000019270 ammonium chloride Nutrition 0.000 description 2
- 229960001040 ammonium chloride Drugs 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 229920001903 high density polyethylene Polymers 0.000 description 2
- 239000004700 high-density polyethylene Substances 0.000 description 2
- 229940059406 lactobacillus rhamnosus gg Drugs 0.000 description 2
- 229920001684 low density polyethylene Polymers 0.000 description 2
- 239000004702 low-density polyethylene Substances 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 229960002337 magnesium chloride Drugs 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 239000002808 molecular sieve Substances 0.000 description 2
- 235000019799 monosodium phosphate Nutrition 0.000 description 2
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229940072033 potash Drugs 0.000 description 2
- IOLCXVTUBQKXJR-UHFFFAOYSA-M potassium bromide Chemical compound [K+].[Br-] IOLCXVTUBQKXJR-UHFFFAOYSA-M 0.000 description 2
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 2
- 235000015320 potassium carbonate Nutrition 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 2
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 2
- 239000004317 sodium nitrate Substances 0.000 description 2
- 235000010344 sodium nitrate Nutrition 0.000 description 2
- IATRAKWUXMZMIY-UHFFFAOYSA-N strontium oxide Chemical compound [O-2].[Sr+2] IATRAKWUXMZMIY-UHFFFAOYSA-N 0.000 description 2
- 229940032330 sulfuric acid Drugs 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 201000009840 acute diarrhea Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 229940037003 alum Drugs 0.000 description 1
- 229940103272 aluminum potassium sulfate Drugs 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- QVQLCTNNEUAWMS-UHFFFAOYSA-N barium oxide Inorganic materials [Ba]=O QVQLCTNNEUAWMS-UHFFFAOYSA-N 0.000 description 1
- CSSYLTMKCUORDA-UHFFFAOYSA-N barium(2+);oxygen(2-) Chemical compound [O-2].[Ba+2] CSSYLTMKCUORDA-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000011229 interlayer Substances 0.000 description 1
- 229920000092 linear low density polyethylene Polymers 0.000 description 1
- 239000004707 linear low-density polyethylene Substances 0.000 description 1
- 239000000395 magnesium oxide Substances 0.000 description 1
- 229920001179 medium density polyethylene Polymers 0.000 description 1
- 239000004701 medium-density polyethylene Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- GRLPQNLYRHEGIJ-UHFFFAOYSA-J potassium aluminium sulfate Chemical compound [Al+3].[K+].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O GRLPQNLYRHEGIJ-UHFFFAOYSA-J 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 239000011833 salt mixture Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 229920001862 ultra low molecular weight polyethylene Polymers 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B15/00—Layered products comprising a layer of metal
- B32B15/20—Layered products comprising a layer of metal comprising aluminium or copper
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/12—Antidiarrhoeals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/12—Drugs for disorders of the metabolism for electrolyte homeostasis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/173—Reuteri
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B15/00—Layered products comprising a layer of metal
- B32B15/04—Layered products comprising a layer of metal comprising metal as the main or only constituent of a layer, which is next to another layer of the same or of a different material
- B32B15/08—Layered products comprising a layer of metal comprising metal as the main or only constituent of a layer, which is next to another layer of the same or of a different material of synthetic resin
- B32B15/085—Layered products comprising a layer of metal comprising metal as the main or only constituent of a layer, which is next to another layer of the same or of a different material of synthetic resin comprising polyolefins
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B2307/00—Properties of the layers or laminate
- B32B2307/70—Other properties
- B32B2307/724—Permeability to gases, adsorption
- B32B2307/7242—Non-permeable
- B32B2307/7246—Water vapor barrier
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B2439/00—Containers; Receptacles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B27/00—Layered products comprising a layer of synthetic resin
- B32B27/18—Layered products comprising a layer of synthetic resin characterised by the use of special additives
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B27/00—Layered products comprising a layer of synthetic resin
- B32B27/32—Layered products comprising a layer of synthetic resin comprising polyolefins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Polymers & Plastics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Food Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Nutrition Science (AREA)
- Diabetes (AREA)
- Hematology (AREA)
- Obesity (AREA)
- Packages (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medical Preparation Storing Or Oral Administration Devices (AREA)
Abstract
本发明提供了对水分敏感的产品的包装方法以延长这种产品的保质期，更具体地，延长与口服再水化溶液(ORS)粉剂混合的冻干的乳酸菌的保质期。所述包装包括两种干燥剂，一种干燥剂整合在箔材料中，第二种干燥剂是无水的ORS。
Description
技术领域
本发明通常涉及对水分敏感的产品的包装，以延长这种产品的保质期。更具体地，本发明涉及延长与口服再水化溶液(ORS)粉剂混合的冻干乳酸菌的保质期。所述产品是包括两种干燥剂的包装：一种干燥剂整合到箔材料中，第二种干燥剂是无水的ORS。本发明还涉及生产这种产品的方法。
技术背景
益生菌是通过改善宿主的肠道的微生物平衡来有益地影响宿主的活的微生物食品添加物。现今，许多种不同的细菌被用作益生菌，例如，产乳酸细菌，例如乳杆菌(Lactobacillus)和双歧杆菌(Bifidobacteria)的菌株。产乳酸细菌不仅因为它们对人类或动物健康的有益效果而被使用，它们还在食品工业中广泛地用于发酵过程。一般地，为此目的销售的微生物被制成处于低水环境中的冻干粉剂。
在这种冻干微生物制品的应用中遇到的一般性难题是细胞的有限的保存稳定性。例如，随着时间的过去，微生物变得少数可存活，导致必需使用高剂量来补偿这种活性损失。
一般已知的是利用铝箔作为包装材料用于微生物的保藏，最优选的箔具有在隔层外侧的一个表面上的塑料层，例如，层压聚乙烯的铝箔，作为包装材料以减少冻干的乳酸菌对水分和氧气的暴露。
虽然常规包装的防潮层在限制水分进入包装的运动方面可能是有用的，但是某些水分分子仍然可以找到途径进入包装，有害地影响其中包含的产品。此外，即使阻挡材料在限制水分子通过包装的输送方面是有效的，包装的某些特征仍然允许水分子的输送，例如，沿着热密封的包装的边缘。并且，就水分吸收而言，包装冻干微生物的时刻是特别弱的时点。
维持包装内特别低的或者几乎不存在的水分水平的一种方案是将小袋的干燥剂材料包裹入包装的内部间隙以从包装的顶部空间移除水分。小袋中含有的干燥剂材料一般为粉剂或颗粒形式，可能从小袋漏出，或者溢出，从而污染产品或包装内含有的产品。市场上已知的可摄取的干燥剂不适合于例如患有腹泻的人。相反，它可能产生有害影响。然而，在此本发明解决了这个问题，其中在小袋内部的干燥剂的目的是消耗所述干燥剂。
典型的干燥剂材料是“物理”干燥剂材料，例如，分子筛，其在材料的孔隙空间内结合水分子。一般地，物理干燥剂材料在所有湿度水平下吸收水，但是当物理干燥剂的空隙被填满时将停止吸水。因而，物理干燥剂材料在高湿度水平下可能是无效的。
另一类干燥剂材料是水化物形成试剂，例如盐。可以用作干燥剂材料的典型的盐是硫酸镁、磷酸二氢钠、氯化铵、碳酸钾、硫酸铝钾、氯化镁、硫酸二铵、硝酸钠、氯化钙和硫酸钙，许多其他的也是已知的。这些材料的干燥能力大大地受到包装内的相对湿度的影响。一般地，直到相对湿度达到第一水化物形成的值时，水化物形成试剂才会吸水。例如对于氯化钙来说，在小于约百分之二的相对湿度(RH)下产生第一水化物。然后水化物形成盐吸水直到第一水化物由盐完全地形成。直到相对湿度达到第二水化物形成的第二水平，盐不会进一步吸收水。这个过程一直继续到试剂形成许多水化物，在此时物质开始溶解并形成饱和溶液。饱和溶液然后将继续吸水。
虽然这些盐在从包装顶部空间内含有的一定数量气体中移除水分子方面是有效的，但是由于盐仅在盐内部结合水分子，水分子可能容易逃逸回到包装中。这被称为呼吸，可能导致包装内的潮解(水滴和液化)。一般地，在包装的装运或保存期间可能发生的，如果盐变得饱和，以及包装的温度升高或者包装的压力降低，可能发生包装内的潮解。
此外，盐不能容许包装内的水分水平降至保护包装内包含的水分敏感性产品所必需的水平。一般地，由于盐具有不同的水化水平，在水化改变之前，湿度水平可能保持在一定水平而不会降低。可以利用这些盐来维持包装顶部空间内一定的湿度水平。例如，某些产品可能需要在包装顶部空间内维持一定水平的水分或湿度。产品的顶部空间湿度控制可以通过掺入合适的水化物形成试剂来控制。
还可以使用不形成水化物的干燥剂材料，例如，食盐(NaCl)或溴化钾(KBr)。例如，食盐将在低于约百分之75的相对湿度下不吸水。当达到百分之75的相对湿度时，形成饱和溶液，其继续吸水。
另一种干燥剂利用化学干燥剂技术。化学干燥剂材料一般在所有湿度水平下吸收水，在高相对湿度水平下将继续吸水。美国专利申请No.20070160789A1描述了多层的塑料聚合柔性包装箔，具有包括在箔层内的化学干燥剂物质。箔优选地是选自由超低密度聚乙烯、低密度聚乙烯、线性低密度聚乙烯、中密度聚乙烯和高密度聚乙烯构成的组的聚乙烯。本发明利用这样的干燥剂，如氧化钙(优选的)、氧化镁、氧化钡、氧化锶、氧化铝、部分水化的氧化铝、硫酸镁、磷酸二氢钠、氯化铵、碳酸钾、硫酸钾铝、氯化镁、硫酸二铵、硝酸钠、氯化钙、硫酸钙、氯化钠、溴化钾、分子筛、粘土，或对本发明有用的任何其他干燥剂材料。
口服再水化治疗(ORT)是用于与腹泻相关的脱水的简单、便宜和有效的治疗。ORT由口服施用的盐和糖溶液组成，也称为口服再水化溶液(ORS)或口服再水化制剂(ORF)。ORT在全世界使用，但最重要的是在发展中世界，在此它将数百万儿童从由于腹泻的死亡中拯救出来，腹泻是5岁以下儿童死亡的第二大主因。
已经进行了几项实验来评估在口服再水化溶液中施用的乳酸菌的功效。例如，进行了多中心的试验来评估向所有病因的急性发作腹泻的患者在口服再水化溶液中施用的乳杆菌GG(Lactobacillus GG)的功效，显示了向患有急性腹泻的儿童施用含有乳杆菌GG的口服再水化溶液是安全的，产生更短的腹泻延续时间、延长病程的几率更低，以及更快的出院(J Pediatr Gastroenterol Nutr，2000Jan；30(1)：54-60)。然而，如前面提及的，难题是冻干的乳酸菌需要在粉剂基质中的低水活性(通常αw＜0.2)，在没有活性的显著损失的情况下，以在环境温度下具有合理的保质期。
由于口服再水化溶液粉剂的极度吸湿的(从周围环境吸引水分子的能力)性质，迄今为止不可能将冻干的乳酸菌与ORS粉剂混合并在环境保存期间维持细菌存活较长的时间。活的益生培养物可变的保存质量引起向急性水泻患者施用的制品中剂量和活性的不可预见的损失，特别是在发展中国家这是难以解决的技术难题。
从保存的角度，在本发明中测试了一种方法来评估与冻干的路氏乳杆菌(Lactobacillus reuteri)混合，是否有任何盐-混合物可以作为包装袋内的干燥剂成功地起作用。包装袋由US20070160789A1中描述的箔材料制成。令人惊讶地，与无水的ORS混合的路氏乳杆菌(L.reuteri)的冻干的培养物成功地达到了30℃下至少12个月的保质期，已知的是意味着在更低的温度下更长的保存时间。这种类型的有益的保存稳定性迄今为止没有被描述。就我们所知，在世界上没有这样的产品出售。
总之，迄今为止不可能将冻干的乳酸菌与ORS粉剂混合并仍然在环境保存期间保持细菌在任何形式的包装中存活持续更久的时间。在此本发明通过用两种不同的干燥剂两个步骤除去水分，解决了这个难题。第一种干燥剂是作为干燥剂起作用的无水的ORS，在包装时围绕着微生物，第二种干燥剂处在箔中吸收在保存期间可能出现的水分。当使用无水的ORS时，它作为不需要移除的干燥剂起作用，同时它满足了其作为口服再水化溶液的目标。
发明内容
本发明提供了对水分敏感的产品的包装方法，从而延长这种产品的保质期，更具体地，延长与口服再水化溶液(ORS)粉剂混合的冻干乳酸菌的保质期。所述包装包含两种干燥剂：一种干燥剂整合在箔材料中，第二种干燥剂是无水的ORS。
本发明的主要目的是提供延长与口服再水化溶液(ORS)粉剂混合的冻干乳酸菌的保质期的产品。
本发明的另一个目的是提供用于冻干的乳酸菌的保存的、在两个步骤中移除水分的两种干燥剂的包装。
另一个目的是提供具有两种干燥剂的小袋：一种干燥剂整合到如US20070160789A1中描述的箔材料中，另一种干燥剂是在围绕产品的、在小袋内自由存在的无水ORS。
本发明的其他目的和优点对于读者将变得显而易见，意图是这些目的和优点处在本发明的范围之内。
附图简要描述
图1显示了在+30℃下每个月直到12个月的使用不同的干燥剂的路氏乳杆菌的生存力的曲线图。
1)路氏乳杆菌+具有干燥剂的箔
2)路氏乳杆菌+具有干燥剂的箔+无水ORS
3)路氏乳杆菌+具有干燥剂的箔+非无水ORS
4)路氏乳杆菌+没有干燥剂的箔+无水ORS
5)路氏乳杆菌+没有干燥剂的箔+非无水ORS
发明及其优选实施方式的详细说明
本发明的方法和设备提供了对水分敏感的产品的包装，从而延长这种产品的保质期，更具体地，延长与口服再水化溶液(ORS)粉剂混合的冻干乳酸细菌的保质期。所述包装包括两种干燥剂：一种干燥剂整合到铝箔中，第二种干燥剂是无水的ORS。所述箔优选地由铝制成，具有适合的保护包被例如聚乙烯或漆。理由是，铝箔是已知最好的针对水分的阻挡物，在此讨论的产品对水分非常敏感。包装的特征性的阻挡物的测试如本领域已知的来进行(Allinson et al，InternationalJournal of Pharmaceutics Volume 221，Issues 1-2，19June 2001，Pages49-5)。
如在此使用的术语“无水的ORS”是指，ORS粉剂的成分是无水性质的，即，没有水。成分可以通过本领域已知的方法变为无水的形式或无水地制备。
在此本发明的目标是提供方法和设备，其使得冻干的乳酸菌存活到以约+30℃的保存12个月的末尾，在这个温度下和起始点，有至少约10E+07CFU/克(当以5×10E+09CFU/克开始时)但优选的至少10E+08CFU/克，最好的10E+09CFU/克。当产品保存在+25℃时，在大约18个月时，这种稳定性已知转变相同损失数量的最大值，当温度更低时则更少。这将意味着，在+25℃下在18个月时的目标，在这个温度下和起始点，有至少10E+07CFU/克(当以5×10E+09CFU/克开始时)，但是优选的至少10E+08CFU/克，以及最好的10E+09CFU/克。
通过参考以下实施例，将更清楚地理解本发明的特征，实施例不被看作是限制本发明。
实施例1
测试的参数
在此处的实施例中，测试本发明的方法来评估ORS溶液盐是否可以与冻干的路氏乳杆菌在小袋中混合，目的是实现在30℃下至少12个月的保质期，作为一种加速的研究，已知的是这意味着在更低温度下更久的保存时间。新的生产方法包括使用具有在US 20070160789A1中描述的干燥剂的新的箔材料。
由五种不同的测试参数组成的试验如表1中所示：
表1
*具有整合在US20070160789A1中描述的干燥剂的箔
**没有整合的干燥剂的参考箔
实施例2
包括处于无水O.R.S.粉剂中路氏乳杆菌的混合物的小袋的生产
无水的ORS粉剂含有：路氏乳杆菌DSM 17938；68.000mg/小袋，约10E+11CFU/克
无水葡萄糖：3781.000mg/小袋
无水柠檬酸钠：866.000mg/小袋
氯化钾：379.000mg/小袋
氯化钠：365.000mg/小袋
硫酸锌：4,000mg/小袋
总共：5463.000mg/小袋
在BioGaia Lund的微生物学实验室中，在LAF工作台(来自Heto-Holten A/S，Denmark的Holten Lamiriair Model S-2010 1.2)上，共混物在环境温度下填充到具有干燥剂的铝箔袋中(10cm×12cm，使用来自Alcan的包装材料PET12/PE/ALU 12/PE/PE+干燥剂/PE)。向每个袋中，使用来自Denver Instrument GmbH，Germany的平衡XP-600添加5.46g的具有路氏乳酸杆菌的ORS粉剂。填充的铝箔袋然后用来自Kettenbaum Folienschweisstechnik GmbH&Co.KG.Germany的箔密封设备型号F460/2热密封。由于实际上完全没有通过铝箔的水分穿透，特别是如果铝厚于0.025mm，因而最优选的是，封闭是完全封闭的敏感部分，这意味着使用的密封设备必需有高质量，得到完整的密封而没有任何渗漏，并且是如本领域已知的充分的。这些袋子也用于实施例2中描述的研究中。
实施例3
在30℃下在12个月期间，在口服再水化溶液(ORS)粉剂中保存期间冻干的路氏乳杆菌DSM17938的稳定性研究
在来自Norfoods AB，Sweden的口服再水化溶液粉剂中评估路氏乳杆菌DSM 17938粉剂art no 1012 BioGaia AB，Stockholm，Sweden(产品规格PS137)的生存力。无水ORS粉剂根据实施例1制得。测验参数根据表1进行。样品保存在Lund Sweden的BioGaia AB处30℃/RH的环境柜中。
实施例4
结果在图1中示出。保存在具有整合的干燥剂和无水ORS的箔中的微生物令人惊讶地(附图1中的曲线2)显示了与没有ORS而保存的微生物几乎相同的生存力(附图1中的曲线1)。
与保存在没有干燥剂的箔中的微生物相比，保存在具有整合的干燥剂的箔中的微生物显示了保存期间更好的生存力，因为箔中的干燥剂在保存期间吸收了袋子中几乎所有的水分。但是仅具有干燥剂的箔和非无水的ORS(附图1中的曲线3)显示不是足够的，因为与保存在具有整合的干燥剂和无水的ORS中的微生物(附图1中的曲线2)相比生存力是更差的。
结论性地，单独的无水的ORS或单独的整合干燥剂的箔都不足以为产品提供足够的保质期。包括它们两者的保存一起满足了在保质期末尾的活性目标。
虽然参考具体实施方式描述了本发明，要理解的是，许多变体、修改和实施方式是可能的，因而，所有这样的变体、修改和实施方式被认为处于本发明的精神和范围之内。
Claims (19)
1.一种益生菌产品，所述益生菌产品为：与无水的口服再水化溶液粉剂混合的冻干的乳酸菌，所述冻干的乳酸菌和所述无水的口服再水化溶液粉剂包装在具有整合到箔中的干燥剂的箔中。
2.如权利要求1所述的益生菌产品，其中，所述箔包括铝。
3.如权利要求2所述的益生菌产品，其中，所述箔进一步包括聚乙烯层。
4.如权利要求1所述的益生菌产品，其中，所述整合到箔材料中的干燥剂包括氧化钙。
5.如权利要求1所述的益生菌产品，其中，所述冻干的乳酸菌是路氏乳杆菌。
6.如权利要求1所述的益生菌产品，其中，所述无水的口服再水化溶液粉剂含有：3781.000mg无水葡萄糖、866.000mg无水柠檬酸钠、379.000mg氯化钾、365.000mg氯化钠以及4000mg硫酸锌。
7.如权利要求1所述的益生菌产品，其中，在30℃下所述益生菌产品的12个月保存之后，当以5×10E+09CFU/克的路氏乳杆菌开始时，存在至少10E+07CFU/克的路氏乳杆菌。
8.如权利要求7所述的益生菌产品，其中，在12个月的保存后存在至少10E+08CFU/克的路氏乳杆菌。
9.如权利要求8所述的益生菌产品，其中，在12个月的保存后存在10E+09CFU/克的路氏乳杆菌。
10.一种用于对水分敏感的产品的活性保存的产品，所述产品为无水的口服再水化溶液粉剂，所述无水的口服再水化溶液粉剂用于与所述对水分敏感的产品混合和用于所述对水分敏感的产品的包装，以及掺合在箔层内的化学干燥剂材料；其中所述对水分敏感的产品包括活的冻干乳酸菌。
11.如权利要求10所述的产品，其中，所述箔包括铝。
12.如权利要求11所述的产品，其中，所述箔进一步包括聚乙烯层。
13.如权利要求10所述的产品，其中，所述无水的口服再水化溶液粉剂含有：3781.000mg无水葡萄糖、866.000mg无水柠檬酸钠、379.000mg氯化钾、365.000mg氯化钠以及4000mg硫酸锌。
14.一种活的冻干益生菌乳酸菌的长期保存的方法，所述方法包括：
a)提供无水的口服再水化溶液粉剂；
b)混合冻干的乳酸菌和所述无水的口服再水化溶液粉剂，
c)在掺入到箔层之内的化学干燥剂材料的包装中，包装所述混合的冻干乳酸菌和口服再水化粉剂；以及
d)密封所述包装使得所述密封是完整的。
15.如权利要求14所述的方法，其中，所述箔包括铝箔。
16.如权利要求15所述的方法，其中，所述箔进一步包括聚乙烯层。
17.如权利要求14所述的方法，其中，所述整合到箔材料中的干燥剂包括氧化钙。
18.如权利要求14所述的方法，其中，所述冻干的乳酸菌是路氏乳杆菌。
19.如权利要求14所述的方法，其中，所述无水的口服再水化溶液粉剂含有：3781.000mg无水葡萄糖、866.000mg无水柠檬酸钠、379.000mg氯化钾、365.000mg氯化钠以及4000mg硫酸锌。
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US27056609P | 2009-07-10 | 2009-07-10 | |
| US61/270,566 | 2009-07-10 | ||
| US12/803,758 | 2010-07-06 | ||
| US12/803,758 US20110014324A1 (en) | 2009-07-10 | 2010-07-06 | Product for the storage of freeze-dried lactic acid bacteria mixed with oral rehydration solution |
| PCT/EP2010/059856 WO2011003995A1 (en) | 2009-07-10 | 2010-07-09 | Product for the storage of freeze-dried lactic acid bacteria mixed with a powder for an oral rehydration solution |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102427738A CN102427738A (zh) | 2012-04-25 |
| CN102427738B true CN102427738B (zh) | 2014-12-17 |
Family
ID=43428822
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201080021605.9A Active CN102427738B (zh) | 2009-07-10 | 2010-07-09 | 用于保存与用于口服再水化溶液的粉剂混合的冻干的乳酸菌的产品 |
Country Status (25)
| Country | Link |
|---|---|
| US (2) | US20110014324A1 (zh) |
| EP (1) | EP2451298B1 (zh) |
| JP (1) | JP5608227B2 (zh) |
| KR (1) | KR101367507B1 (zh) |
| CN (1) | CN102427738B (zh) |
| AU (1) | AU2010270221B2 (zh) |
| BR (1) | BRPI1014873B1 (zh) |
| CA (1) | CA2760008C (zh) |
| CL (1) | CL2011002644A1 (zh) |
| DK (1) | DK2451298T3 (zh) |
| ES (1) | ES2421302T3 (zh) |
| HK (1) | HK1169792A1 (zh) |
| HR (1) | HRP20130550T1 (zh) |
| IL (1) | IL216544A (zh) |
| MA (1) | MA33370B1 (zh) |
| MX (1) | MX2012000508A (zh) |
| NZ (1) | NZ595886A (zh) |
| PL (1) | PL2451298T3 (zh) |
| PT (1) | PT2451298E (zh) |
| RS (1) | RS52846B (zh) |
| RU (1) | RU2527515C2 (zh) |
| SG (1) | SG175284A1 (zh) |
| SI (1) | SI2451298T1 (zh) |
| WO (1) | WO2011003995A1 (zh) |
| ZA (1) | ZA201107697B (zh) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20170066395A (ko) * | 2014-10-02 | 2017-06-14 | 프로바이오티컬 에스.피.에이. | 식품, 음식물 및 화장품, 의료 기기 및 의약품을 포장하기 위한 자유수를 흡수 및 보유할 수 있고, 흡수된 자유수를 방출하지 않는 완전 수밀 다층 재료 |
| JP6584777B2 (ja) * | 2015-01-16 | 2019-10-02 | 太陽化学株式会社 | 水分電解質補給飲料用粉末組成物 |
| CN111513331A (zh) * | 2020-04-16 | 2020-08-11 | 仙乐健康科技股份有限公司 | 一种包含益生菌的产品 |
| CN113398874B (zh) * | 2021-06-17 | 2023-08-15 | 干霸干燥剂(深圳)有限公司 | 一种复合干燥剂及其制备方法 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070160789A1 (en) * | 2002-06-20 | 2007-07-12 | Rick Merical | Films having a desiccant material incorporated therein and methods of use and manufacture |
| CN101225365A (zh) * | 2007-01-18 | 2008-07-23 | 马小明 | 一种复合菌种发酵剂及其制备方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4107335A (en) * | 1977-04-08 | 1978-08-15 | The United States Of America As Represented By The Secretary Of The Army | Freeze-dried mix for spoonable salad dressing and method therefor |
| US5837238A (en) * | 1996-06-05 | 1998-11-17 | Biogaia Biologics Ab | Treatment of diarrhea |
| US7374753B1 (en) * | 1997-06-03 | 2008-05-20 | Ganeden Biotech, Inc. | Probiotic lactic acid bacterium to treat bacterial infections associated with SIDS |
| JPH114666A (ja) * | 1997-06-17 | 1999-01-12 | Otsuka Pharmaceut Co Ltd | マグネシウム補給発泡剤組成物 |
| US20030006159A1 (en) * | 1999-09-01 | 2003-01-09 | Jorgen Thorball | Additive tube for enteral nutrition apparatus |
| US7026298B2 (en) * | 2001-06-04 | 2006-04-11 | Abbott Laboratories | Oral rehydration compositions |
| WO2003082027A1 (fr) * | 2002-03-29 | 2003-10-09 | Frente International Co., Ltd. | Preparations cellulaires vitales et aliments contenant une bacterie lactique comme principe actif |
| US20050281756A1 (en) * | 2004-06-14 | 2005-12-22 | Eamonn Connolly | Use of lactic acid bacteria for decreasing gum bleeding and reducing oral inflammation |
| JP3949641B2 (ja) * | 2003-11-04 | 2007-07-25 | 株式会社林原生物化学研究所 | トレハロース粉末とその製造方法 |
| JP4766485B2 (ja) * | 2006-03-06 | 2011-09-07 | 四国乳業株式会社 | 新規乳酸菌 |
| US7566463B2 (en) * | 2006-05-03 | 2009-07-28 | C. B. Fleet Company | Oral rehydration compositions |
| US7560364B2 (en) * | 2006-05-05 | 2009-07-14 | Applied Materials, Inc. | Dislocation-specific lateral epitaxial overgrowth to reduce dislocation density of nitride films |
| WO2008119012A2 (en) * | 2007-03-27 | 2008-10-02 | The Procter & Gamble Company | Methods and kits for administering probiotics |
| US20090061222A1 (en) * | 2007-08-31 | 2009-03-05 | Tesa Aktiengesellschaft | Multi-layer adhesive closure |
| NZ555022A (en) * | 2007-11-07 | 2010-09-30 | Encoate Holdings Ltd | Stabilisation of dried biological material with oil and biopolymer |
-
2010
- 2010-07-06 US US12/803,758 patent/US20110014324A1/en not_active Abandoned
- 2010-07-09 DK DK10730463.6T patent/DK2451298T3/da active
- 2010-07-09 RS RS20130262A patent/RS52846B/en unknown
- 2010-07-09 AU AU2010270221A patent/AU2010270221B2/en not_active Ceased
- 2010-07-09 MX MX2012000508A patent/MX2012000508A/es active IP Right Grant
- 2010-07-09 KR KR1020117025729A patent/KR101367507B1/ko active IP Right Grant
- 2010-07-09 MA MA34467A patent/MA33370B1/fr unknown
- 2010-07-09 NZ NZ595886A patent/NZ595886A/xx not_active IP Right Cessation
- 2010-07-09 EP EP10730463.6A patent/EP2451298B1/en active Active
- 2010-07-09 CN CN201080021605.9A patent/CN102427738B/zh active Active
- 2010-07-09 ES ES10730463T patent/ES2421302T3/es active Active
- 2010-07-09 JP JP2012519004A patent/JP5608227B2/ja active Active
- 2010-07-09 PT PT107304636T patent/PT2451298E/pt unknown
- 2010-07-09 PL PL10730463T patent/PL2451298T3/pl unknown
- 2010-07-09 CA CA2760008A patent/CA2760008C/en active Active
- 2010-07-09 RU RU2011151392/13A patent/RU2527515C2/ru active
- 2010-07-09 SG SG2011076841A patent/SG175284A1/en unknown
- 2010-07-09 SI SI201030242T patent/SI2451298T1/sl unknown
- 2010-07-09 BR BRPI1014873-6A patent/BRPI1014873B1/pt active IP Right Grant
- 2010-07-09 WO PCT/EP2010/059856 patent/WO2011003995A1/en active Application Filing
-
2011
- 2011-10-20 ZA ZA2011/07697A patent/ZA201107697B/en unknown
- 2011-10-24 CL CL2011002644A patent/CL2011002644A1/es unknown
- 2011-11-23 IL IL216544A patent/IL216544A/en active IP Right Grant
-
2012
- 2012-10-24 HK HK12110560.5A patent/HK1169792A1/zh not_active IP Right Cessation
-
2013
- 2013-06-17 HR HRP20130550AT patent/HRP20130550T1/hr unknown
-
2023
- 2023-08-16 US US18/450,498 patent/US20240109273A1/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070160789A1 (en) * | 2002-06-20 | 2007-07-12 | Rick Merical | Films having a desiccant material incorporated therein and methods of use and manufacture |
| CN101225365A (zh) * | 2007-01-18 | 2008-07-23 | 马小明 | 一种复合菌种发酵剂及其制备方法 |
Non-Patent Citations (3)
| Title |
|---|
| A trial in the Karelian Republic of oral rehydration and Lactobacillus GG;SHORNIKOVA A V, ET ALL;《ACTA PAEDIATRICA》;19970501;第86卷(第5期);第460-465页 * |
| Lactobacillus GG administered in oral rehydration solution to children with acute diarrhea:a multicenter European trial;Guandalini,Stefano,et al;《JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION》;20000101;第30卷(第1期);第54-60页 * |
| Sriparna Basu,et al.Efficacy of high-dose Lactobacillus rhamnosus GG in controlling acute watery diarrhea in Indian children.《JOURNAL OF CLINICAL GASTROENTEROLOGY》.2009,第43卷(第3期),第208-213页. * |
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20240109273A1 (en) | Product for the Storage of Freeze-Dried Lactic Acid Bacteria Mixed with Oral Rehydration Solution | |
| JP6211146B2 (ja) | 乳酸菌及び乾燥剤を含む産物 | |
| CN1123381C (zh) | 一种化学吸氧剂及其制备方法 | |
| CN101002626A (zh) | 中药材贮藏用除氧剂 | |
| CN103416435B (zh) | 药用防霉剂及其制备方法 | |
| CN1085496C (zh) | 缓释放防霉保鲜方法及装置 | |
| CN1515471A (zh) | 含厌氧菌产品的室温保存方法及用该方法包装的产品 | |
| CN104192431B (zh) | 一种自然耗氧气调包装技术 | |
| KR20230054921A (ko) | 포장등 밀키트 | |
| JP2003267456A (ja) | 酸素吸収性密封容器の膨れ防止方法 | |
| JP2002051697A (ja) | 発酵漬け物の保存方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 1169792 Country of ref document: HK |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: GR Ref document number: 1169792 Country of ref document: HK |