CN102334599A - Method for producing livestock nutrient solution by fermenting flaxseed cake meal - Google Patents
Method for producing livestock nutrient solution by fermenting flaxseed cake meal Download PDFInfo
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Abstract
The invention discloses a method for producing livestock nutrient solution by fermenting flaxseed cake meal, which includes the following steps: (1) material choice; (2) soaking; (3) microwave sterilization and disinfection; (4) enzymolysis; (5) fermentation; (6) filtration; (7) high-pressure homogenization. The method has the advantages that: the method which takes the affection of the components contained in the flaxseed cake meal on the livestock nutrient solution product into full consideration utilizes microwaves to sterilize and disinfect the flaxseed cake meal, adds compound enzyme and complex bacteria to ferment the flaxseed cake meal, and homogenizes the different components of flaxseed cake meal fermentation broth with high pressure, consequently, bacterial cell walls can be effectively broken, organic matters can be homogenized, the nutrients of the fermentation broth can be balanced, and the produced livestock nutrient solution is then bottled. The method has the advantages of high speed, high yield and the like, the dissoluble solid content of the livestock nutrient solution can reach 6 to 10 percent, energy can be saved by 20 to 30 percent, and the method can be widely applied to the production of livestock nutrient solution with proteide flaxseed cake meal, rapeseed cake meal and the like as fermented materials.
Description
Technical field
The present invention relates to a kind of production method of raiseeing, be specifically related to the method for a kind of linseed grouts fermenting and producing poultry with nutrient solution with nutrient solution.
Background technology
Linseed cake is to adopt mechanical hot moulding oil process squeezing linseed to remove the remaining dregs of fat after the linseed oil, and China produces about 26.5 ten thousand tons of linseed grouts per year.The linseed grouts are except that containing protein 32-36%; Crude fiber content can reach 8-28%, and crude fibre itself can not be utilized by the simple stomach animal digestion, and cellulose parcel nutriment; Obstruction fully contacts with digestive ferment; Reduce the utilization rate of nutrient in the feed, and can generate insoluble salt, influence absorbing of mineral element with metal ion generation chelatropic reactions such as calcium in the daily ration, copper, iron, zinc.Influence animal digesting and assimilating to other nutriment.Owing to contain living cyanogen glucoside in the linseed grouts, after the cyanogen glucoside gets into body, can under the effect of lipolytic enzyme, hydrolysis produce hydrogen cyanide and cause the poisoning of livestock and poultry in addition.When linseed cake was made feed, treatment method for detoxication commonly used was: linseed cake is pulverized, added 4-5 times of warm water; Soak that drop anhydrates after 8-12 hour, add proper amount of clear water again and boiled 1 hour, when boiling, constantly stir and open wide pot cover; Add vinegar simultaneously, so that hydrogen cyanide volatilization as far as possible.Because traditional feed processing technology can not guarantee that the linseed grouts are as quality of the fodder.The linseed grouts replenish the protein except that being utilized on a small quantity as feed, go out of use in a large number, have caused the very big wasting of resources.At present, linseed grouts comprehensive processing and utilization has become flax industry development problem anxious to be solved.
The poultry of linseed grouts fermenting and producing is meant in containing the linseed grouts of 36% protein with nutrient solution adds complex enzyme, complex micro organism fungicide; Fermentation linseed grouts; Filtration is processed contains lysine 1.16%, methionine 0.55%, and arginine reaches 3.59%; Fat 1.8%, the zymotic fluid of a large amount of mycoproteins and small amounts of inorganic ion.The linseed grouts contain protein 32-36%, cellulose 8-28% and a small amount of cyanogen glucoside of giving birth to.Owing to contain the cyanogen glucoside,, often cause livestock and poultry to poison directly as feed.Protein in the linseed grouts but is rare livestock and poultry proteins additive, required nutriment when being growth of animals or poultry.In the prior art; Biological nutrient liquid (farming is happy) and manufacture craft thereof; The number of applying for a patent 00103376, this biological nutrient liquid (farming happy) are mainly processed by raw material such as the trace element of elements such as amino acid, cupric zinc-iron, urea, ammoniacal liquor, potassium hydroxide, lactic acid, sorbierite and an amount of water.The deficiency of the method is: in the preparation of raw material process, the influence that trace elements such as copper zinc-iron are subject to potassium hydroxide precipitates, and is difficult for being absorbed by animal; Amino acid receives lactic acid to influence nutritional labeling to be underutilized in the solution; Ammoniacal liquor, potassium hydroxide destructible soil acid-base equilibrium suppress plant growth.High efficiency animal nutrient solution and preparation method thereof; The number of applying for a patent 200710122614; Nutrient solution is by compound amino acid, purple perilla extract, alizarin powder, grape pip powder, titanium tetrachloride, manganese sulfate, zinc sulfate, multienzyme body hydrolase, boric acid, calcium gluconae, dipotassium hydrogen phosphate, ferrous sulfate; Cobastab, vitamin A; Yeast, cobalt carbonate, strontium chloride, natrium nitrosum, chitin, nucleic acid, ammonium molybdate; Compositions such as albumen freezes, oligosaccharides, water.Through industrial fermentation, in protein, extract amino acid and form organic chelate with the metallic element titanium ion.The deficiency of the method is: material liquid contains the fermentation that a large amount of inorganic ions suppress microorganisms such as yeast, and the nutritional labeling that contains in the zymotic fluid is underutilized; Add multiple sulfate in the zymotic fluid, have the mutual inhibitory action of ion, effective element is restricted with the trophism of trace element.
Summary of the invention
The object of the invention is exactly to above-mentioned defective of the prior art, and a kind of method that can make full use of material effective component, nutrient composition content is high, output capacity is high, with short production cycle, energy-efficient linseed grouts fermenting and producing poultry with nutrient solution is provided.
To achieve these goals, technical scheme provided by the invention is: a kind of linseed grouts fermenting and producing poultry includes following steps with the method for nutrient solution:
1) material choice: the screening protein content is that the linseed grouts of 32-36% are raw material;
2) soak: the linseed grouts raw material that the step 1) screening is obtained is according to weight ratio linseed grouts raw material: water is that 1:1.5-2 mixes, and temperature 15-25 ℃, soaks 60-120min and obtains linseed grouts material liquid;
3) the linseed grouts material liquid 500-1500g that microwave sterilization detoxification: with step 2) obtains carries out microwave sterilization; Microwave power is 900w, and the time is 10-20min, afterwards with its taking-up and be cooled to 15-25 ℃; The hydrogen cyanide that sheds obtains detoxification linseed grouts material liquid;
4) enzymolysis: in the detoxification linseed grouts material liquid that step 3) obtains, add cellulase, pectase and protease; Addition is according to weight ratio detoxification linseed grouts material liquid: cellulase: pectase: protease is 1000:1-5:3-10:5-15; Mix 40-55 ℃ of back insulation, enzymolysis 24-48h obtains detoxification linseed grouts fermentation raw material liquid;
5) fermentation: in the detoxification linseed grouts fermentation raw material liquid that step 4) obtains, insert the composite bacteria agent capable of percentage by weight 0.1-2%, be incubated 25-32 ℃, fermentation 24-72h obtains detoxification linseed grouts zymotic fluid; In the said composite bacteria agent capable, the ratio of the interpolation weight of Angel Yeast bacterium and the effective viable bacteria of lactic acid bacteria is 1:5;
6) filter: in the detoxification linseed grouts zymotic fluid that step 5) obtains, compress filter press (200 types that Yuzhou City chemical industry equipment Co., Ltd of power produces manually compress filter press) by weight sending into after 1:2 adds entry; Filtration obtains muddy detoxification linseed grouts fermentation filtered fluid, and measures detoxification linseed grouts fermentation filtered fluid soluble solid content;
7) high-pressure homogeneous: the detoxification linseed grouts fermentation filtered fluid that step 6) is obtained; Continuously in the input high pressure homogenizer (the GJB 0.5-60 type that the ultra power homogenizer in changzhou city factory produces); Pressure 25-60MPa, processing speed is 1.5-2.0L/h, obtains linseed grouts poultry and uses nutrition;
8) packing: under the aseptic condition, use nutrient solution, obtain the flaxseed meal poultry and use nutrient solution with vial or the canned 250-1000ml poultry of plastic bottle, wherein, and living bacteria count content 0.2-0.5 hundred million/ml, the linseed grouts are raiseeed with nutrient solution soluble solid content 6-10%.
In the detoxification linseed grouts fermentation filtered fluid that said step 6) obtains, the soluble solid content that records carries out vacuum and concentrates less than 6%; Vacuum is 0.06-0.08MPa; Temperature is 30-45 ℃, and the time is 50-120 min, is concentrated into soluble solid content and reaches 6-10%.
In the detoxification linseed grouts fermentation filtered fluid that said step 6) obtains, the soluble solid content that records is greater than 10%, and thin up is diluted to soluble solid content and reaches 6-10%.
Raw material in the said step 1) is dregs of rapeseed cake, soya bean grouts or sunflower seed dregs.
Beneficial effect of the present invention is:
Linseed grouts fermenting and producing poultry provided by the invention is with the method for nutrient solution; Owing to having adopted the microwave sterilization technology, utilize the inner mode of heating of microwave, make the inside and outside simultaneously even heat sterilization of linseed grouts in the short time; The method is sterilized to the linseed grouts; Speed is fast, the time is short, and can keep the quality of linseed grouts material to greatest extent, simultaneously the energy-conservation 30-50% that reaches.Owing to adopted high pressure homogenization technique, organic matter and somatic cells in the broken linseed grouts of the strong shear action zymotic fluid that produces when utilizing liquid to flow fast, the complete strippings of material such as contained protein, fat in the somatic cells.The method has kept amino acid and vitamins and other nutritious components in the linseed grouts well, and causing death with the bacterium simultaneously is main microorganism, and the material homogenizing effect is good, and sterilization speed is fast.
In sum, the present invention takes into full account the contained composition of linseed grouts to the influence of poultry with nutrition product, utilizes microwave sterilization detoxification linseed grouts; Add complex enzyme, add compound bacteria fermentation linseed grouts; The high-pressure homogeneous heterogeneity that acts on linseed grouts zymotic fluid is effectively abolished somatic cells wall and homogeneous organic matter, balanced zymotic fluid nutrient; Through canned, obtain poultry and use nutrient solution again.This method has fast, the yield advantages of higher of speed, and poultry can reach 6-10% with the nutrient solution soluble solid content, and energy-conservation 20-30% raises for fermenting raw materials production adaptable across proteinaceous linseed grouts, dregs of rapeseed cake etc. and to use nutrient solution.This The Application of Technology has positive effect to the raising of the technology innovation of linseed grouts manufacturing enterprise, enhancing enterprise competitiveness, promotion flax industry comprehensive benefit.
The specific embodiment
Embodiment 1:
1) material choice: the screening protein content is that 32% linseed grouts are raw material;
2) soak: the linseed grouts raw material that the step 1) screening is obtained is according to weight ratio linseed grouts raw material: water is that 1:1.5 mixes, and 25 ℃ of normal temperature soak 60min and obtain linseed grouts material liquid;
3) the linseed grouts material liquid 1500g that microwave sterilization detoxification: with step 2) obtains carries out microwave sterilization, and microwave power is 900w, and the time is 20min, and afterwards with its taking-up and be cooled to 25 ℃, the hydrogen cyanide that sheds obtains detoxification linseed grouts material liquid;
4) enzymolysis: in the detoxification linseed grouts material liquid that step 3) obtains, add cellulase, pectase and protease; Addition is according to weight ratio detoxification linseed grouts material liquid: cellulase: pectase: protease is 1000:1:10:15; Mix 55 ℃ of back insulations, enzymolysis 24h obtains detoxification linseed grouts fermentation raw material liquid;
5) fermentation: in the detoxification linseed grouts fermentation raw material liquid that step 4) obtains, insert the composite bacteria agent capable of percentage by weight 2%, be incubated 25 ℃, fermentation 72h obtains detoxification linseed grouts zymotic fluid; In the said composite bacteria agent capable, the ratio that the effective viable bacteria of Angel Yeast bacterium and lactic acid bacteria is added weight be 1:5 (w/w, g/g);
6) filter: in the detoxification linseed grouts zymotic fluid that step 5) obtains, manually compress filter press by weight sending into 200 types that Yuzhou City chemical industry equipment Co., Ltd of power produces after 1:2 adds entry; Filtration obtains muddy detoxification linseed grouts fermentation filtered fluid, and mensuration detoxification linseed grouts fermentation filtered fluid soluble solid content is 5.6%;
7) concentrate: the detoxification linseed grouts fermentation filtered fluid that step 6) is obtained is 0.08MPa in vacuum, and temperature is 45 ℃, and the time is 120 min, is concentrated into linseed grouts fermentation filtered fluid soluble solid content and reaches 6.5%.
8) high-pressure homogeneous: step 7) is obtained linseed grouts concentrate, import continuously in the GJB 0.5-60 type high pressure homogenizer of changzhou city ultra power homogenizer factory production, pressure 60MPa, processing speed is 1.5L/h, obtains linseed grouts poultry and uses nutrient solution.
9) packing: under the aseptic condition, use nutrient solution, be product with vial or the canned 1000ml poultry of plastic bottle.Product living bacteria count content 0.5 hundred million/ml, the linseed grouts are raiseeed with nutrient solution soluble solid content 6.5%.
Embodiment 2:
1) material choice: the screening protein content is that 34% linseed grouts are raw material;
2) soak: the linseed grouts raw material that the step 1) screening is obtained is according to weight ratio linseed grouts raw material: water is that 1:1.8 mixes, and 20 ℃ of normal temperature soak 100min and obtain linseed grouts material liquid;
3) the linseed grouts material liquid 1000g that microwave sterilization detoxification: with step 2) obtains carries out microwave sterilization, and microwave power is 900w, and the time is 15min, and afterwards with its taking-up and be cooled to 20 ℃, the hydrogen cyanide that sheds obtains detoxification linseed grouts material liquid;
4) enzymolysis: in the detoxification linseed grouts material liquid that step 3) obtains, add cellulase, pectase and protease; Addition is according to weight ratio detoxification linseed grouts material liquid: cellulase: pectase: protease is 1000:3:6:10; Mix 45 ℃ of back insulations, enzymolysis 30h obtains detoxification linseed grouts fermentation raw material liquid;
5) fermentation: in the detoxification linseed grouts fermentation raw material liquid that step 4) obtains, insert the composite bacteria agent capable of percentage by weight 1%, be incubated 30 ℃, fermentation 48h obtains detoxification linseed grouts zymotic fluid; In the said composite bacteria agent capable, the ratio that the effective viable bacteria of Angel Yeast bacterium and lactic acid bacteria is added weight be 1:5 (w/w, g/g);
6) filter: in the detoxification linseed grouts zymotic fluid that step 5) obtains, manually compress filter press by weight sending into 200 types that Yuzhou City chemical industry equipment Co., Ltd of power produces after 1:2 adds entry; Filtration obtains muddy detoxification linseed grouts fermentation filtered fluid, and mensuration detoxification linseed grouts fermentation filtered fluid soluble solid content is 8.2%;
7) high-pressure homogeneous: the detoxification linseed grouts fermentation filtered fluid that step 6) is obtained; In the GJB 0.5-60 type high pressure homogenizer that input changzhou city ultra power homogenizer factory produces continuously; Pressure 40MPa, processing speed is 1.8 L/h, obtains linseed grouts poultry and uses nutrient solution.
8) packing: under the aseptic condition, use nutrient solution, be product with vial or the canned 500ml poultry of plastic bottle.Product living bacteria count content 0.3 hundred million/ml, the linseed grouts are raiseeed with nutrient solution soluble solid content 8.2%.
Embodiment 3:
1) material choice: the screening protein content is that 36% linseed grouts are raw material;
2) soak: the linseed grouts raw material that the step 1) screening is obtained is according to weight ratio linseed grouts raw material: water is that 1:2 mixes, and 15 ℃ of normal temperature soak 120min and obtain linseed grouts material liquid;
3) the linseed grouts material liquid 500g that microwave sterilization detoxification: with step 2) obtains carries out microwave sterilization, and microwave power is 900w, and the time is 10min, and afterwards with its taking-up and be cooled to 15 ℃, the hydrogen cyanide that sheds obtains detoxification linseed grouts material liquid;
4) enzymolysis: in the detoxification linseed grouts material liquid that step 3) obtains, add cellulase, pectase and protease; Addition is according to weight ratio detoxification linseed grouts material liquid: cellulase: pectase: protease is 1000:5:3:5; Mix 40 ℃ of back insulations, enzymolysis 48h obtains detoxification linseed grouts fermentation raw material liquid;
5) fermentation: in the detoxification linseed grouts fermentation raw material liquid that step 4) obtains, insert the composite bacteria agent capable of percentage by weight 0.1%, be incubated 32 ℃, fermentation 24h obtains detoxification linseed grouts zymotic fluid; In the said composite bacteria agent capable, the ratio that the effective viable bacteria of Angel Yeast bacterium and lactic acid bacteria is added weight be 1:5 (w/w, g/g);
6) filter: in the detoxification linseed grouts zymotic fluid that step 5) obtains, manually compress filter press by weight sending into 200 types that Yuzhou City chemical industry equipment Co., Ltd of power produces after 1:2 adds entry; Filtration obtains muddy detoxification linseed grouts fermentation filtered fluid, and mensuration detoxification linseed grouts fermentation filtered fluid soluble solid content is 10.1%;
7) dilution: with the linseed grouts fermentation filtered fluid that step 6) obtains, thin up to linseed grouts poultry reaches 10% with the nutrient solution soluble solid content.
8) high-pressure homogeneous: the linseed grouts fermentation filtered fluid after the dilution that step 7) is obtained; In the GJB 0.5-60 type high pressure homogenizer that input changzhou city ultra power homogenizer factory produces continuously; Pressure 25MPa, processing speed is 2 L/h, obtains linseed grouts poultry and uses nutrient solution.
9) packing: under the aseptic condition, use nutrient solution, be product with vial or the canned 250ml poultry of plastic bottle.Living bacteria count content 0.2 hundred million/ml, the linseed grouts are raiseeed with nutrient solution soluble solid content 10%.
Embodiment 4:
With the dregs of rapeseed cake is raw material, and the method that using the embodiment of the invention 1 provides is produced poultry and used nutrient solution, difference only to be the raw material difference that step (1) is selected for use.
Embodiment 5:
With the soya bean grouts is raw material, and the method that using the embodiment of the invention 2 provides is produced poultry and used nutrient solution, difference only to be the raw material difference that step (1) is selected for use.
Embodiment 6:
With the sunflower seed dregs is raw material, and the method that using the embodiment of the invention 3 provides is produced poultry and used nutrient solution, difference only to be the raw material difference that step (1) is selected for use.
What should explain at last is: the above is merely the preferred embodiments of the present invention; Be not limited to the present invention; Although the present invention has been carried out detailed explanation with reference to previous embodiment; For a person skilled in the art, it still can be made amendment to the technical scheme that aforementioned each embodiment put down in writing, and perhaps part technical characterictic wherein is equal to replacement.All within spirit of the present invention and principle, any modification of being done, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (4)
1. a linseed grouts fermenting and producing is raiseeed the method with nutrient solution, it is characterized in that: include following steps:
1) material choice: the screening protein content is that the linseed grouts of 32-36% are raw material;
2) soak: the linseed grouts raw material that the step 1) screening is obtained is according to weight ratio linseed grouts raw material: water is that 1:1.5-2 mixes, and temperature 15-25 ℃, soaks 60-120min and obtains linseed grouts material liquid;
3) the linseed grouts material liquid 500-1500g that microwave sterilization detoxification: with step 2) obtains carries out microwave sterilization; Microwave power is 900w, and the time is 10-20min, afterwards with its taking-up and be cooled to 15-25 ℃; The hydrogen cyanide that sheds obtains detoxification linseed grouts material liquid;
4) enzymolysis: in the detoxification linseed grouts material liquid that step 3) obtains, add cellulase, pectase and protease; Addition is according to weight ratio detoxification linseed grouts material liquid: cellulase: pectase: protease is 1000:1-5:3-10:5-15; Mix 40-55 ℃ of back insulation, enzymolysis 24-48h obtains detoxification linseed grouts fermentation raw material liquid;
5) fermentation: in the detoxification linseed grouts fermentation raw material liquid that step 4) obtains, insert the composite bacteria agent capable of percentage by weight 0.1-2%, be incubated 25-32 ℃, fermentation 24-72h obtains detoxification linseed grouts zymotic fluid; In the said composite bacteria agent capable, the ratio of the interpolation weight of Angel Yeast bacterium and the effective viable bacteria of lactic acid bacteria is 1:5;
6) filter: step 5) obtain in compress filter press by weight sending into after 1:2 adds entry, filter and obtain muddy detoxification linseed grouts fermentation filtered fluid, and measure detoxification linseed grouts fermentation filtered fluid soluble solid content;
7) high-pressure homogeneous: with the detoxification linseed grouts fermentation filtered fluid that step 6) obtains, import continuously in the high pressure homogenizer, pressure 25-60MPa, processing speed is 1.5-2 L/h, obtains linseed grouts poultry and uses nutrient solution;
8) packing: under the aseptic condition, use nutrient solution, obtain the flaxseed meal poultry and use nutrient solution with vial or the canned 250-1000ml poultry of plastic bottle, wherein, and living bacteria count content 0.2-0.5 hundred million/ml, the linseed grouts are raiseeed with nutrient solution soluble solid content 6-10%.
2. linseed grouts fermenting and producing poultry according to claim 1 is with the method for nutrient solution; It is characterized in that: in the detoxification linseed grouts fermentation filtered fluid that said step 6) obtains, the soluble solid content that records carries out vacuum and concentrates less than 6%; Vacuum is 0.06-0.08MPa; Temperature is 30-45 ℃, and the time is 50-120 min, is concentrated into soluble solid content and reaches 6-10%.
3. linseed grouts fermenting and producing poultry according to claim 1 is with the method for nutrient solution; It is characterized in that: in the detoxification linseed grouts fermentation filtered fluid that said step 6) obtains; The soluble solid content that records is greater than 10%; Thin up is diluted to soluble solid content and reaches 6-10%.
4. linseed grouts fermenting and producing poultry according to claim 1 is with the method for nutrient solution, and it is characterized in that: the raw material in the said step 1) is dregs of rapeseed cake, soya bean grouts or sunflower seed dregs.
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Cited By (12)
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CN102754765A (en) * | 2012-07-05 | 2012-10-31 | 东北林业大学 | Process for producing peony seed nutrition powder |
CN102823782A (en) * | 2012-09-03 | 2012-12-19 | 内蒙古大学 | Detoxification method of flaxseed product |
CN105918610A (en) * | 2016-04-20 | 2016-09-07 | 武汉天天好生物制品有限公司 | Flaxseed peptides having effects of lowering cholesterol and preparation method thereof |
CN106819437A (en) * | 2017-01-24 | 2017-06-13 | 长治市雄丰清真养殖屠宰有限公司 | It is a kind of to prevent ox, the preparation method of the feed of sheep mouth inflammation disease |
CN107136183A (en) * | 2017-01-22 | 2017-09-08 | 山西省生物研究所 | A kind of red yeast rice Flaxseed cake fermentate and its application |
CN108308585A (en) * | 2018-02-13 | 2018-07-24 | 广州利众生物科技有限公司 | A kind of instant diet linseed meal and preparation method thereof |
CN109007373A (en) * | 2018-09-07 | 2018-12-18 | 唐清池 | A kind of preparation method of novel fermentation gold flaxseed meal |
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CN111602813A (en) * | 2020-05-27 | 2020-09-01 | 华熙生物科技股份有限公司 | Preparation method of sunflower disc fermentation stock solution for health food and product thereof |
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CN102754765A (en) * | 2012-07-05 | 2012-10-31 | 东北林业大学 | Process for producing peony seed nutrition powder |
CN102823782A (en) * | 2012-09-03 | 2012-12-19 | 内蒙古大学 | Detoxification method of flaxseed product |
CN105918610B (en) * | 2016-04-20 | 2019-09-03 | 武汉天天好生物制品有限公司 | A kind of linseed peptide and preparation method thereof with norcholesterol effect |
CN105918610A (en) * | 2016-04-20 | 2016-09-07 | 武汉天天好生物制品有限公司 | Flaxseed peptides having effects of lowering cholesterol and preparation method thereof |
CN107136183A (en) * | 2017-01-22 | 2017-09-08 | 山西省生物研究所 | A kind of red yeast rice Flaxseed cake fermentate and its application |
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PL422849A1 (en) * | 2017-09-14 | 2019-03-25 | Uniwersytet Przyrodniczy W Poznaniu | Method for upgrading rapeseed cakes |
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