CN102321641A - 从烟草克隆细胞色素p450基因 - Google Patents
从烟草克隆细胞色素p450基因 Download PDFInfo
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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Applications Claiming Priority (6)
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| US10/686,947 | 2003-10-16 | ||
| US10/686,947 US20040162420A1 (en) | 2002-10-16 | 2003-10-16 | Cloning of cytochrome p450 genes from nicotiana |
| US56623504P | 2004-04-29 | 2004-04-29 | |
| US60/566,235 | 2004-04-29 | ||
| US10/934,944 US7812227B2 (en) | 2001-11-13 | 2004-09-03 | Cloning of cytochrome p450 genes from nicotiana |
| US10/934,944 | 2004-09-03 |
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| CN2004800303850A Division CN1867674B (zh) | 2003-10-16 | 2004-10-15 | 从烟草克隆细胞色素p450基因 |
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| CN2011102770337A Pending CN102321641A (zh) | 2003-10-16 | 2004-10-15 | 从烟草克隆细胞色素p450基因 |
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| JP (2) | JP5422099B2 (enExample) |
| CN (1) | CN102321641A (enExample) |
| AT (1) | ATE536415T1 (enExample) |
| AU (3) | AU2004282576B2 (enExample) |
| BR (1) | BRPI0415363A (enExample) |
| ES (1) | ES2377868T3 (enExample) |
| PL (1) | PL1675957T3 (enExample) |
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| CN102796821A (zh) * | 2012-08-30 | 2012-11-28 | 浙江中烟工业有限责任公司 | 一种检测烟草烟碱转化相对水平的方法 |
| CN103361368A (zh) * | 2012-03-27 | 2013-10-23 | 华中农业大学 | 一种棉花细胞色素p450基因及应用 |
| CN110305893A (zh) * | 2018-03-23 | 2019-10-08 | 中国科学院上海生命科学研究院 | 棉酚生物合成途径因cyp71be79及其应用 |
| CN110885841A (zh) * | 2019-12-24 | 2020-03-17 | 河南科技大学 | 葡萄VyCYP89A2基因及其编码蛋白和基因在抗旱品种育种中的应用 |
| CN110923249A (zh) * | 2019-10-15 | 2020-03-27 | 贵州省烟草科学研究院 | 烟草CyP71及在调控植物表皮毛发育方面的应用 |
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| CN113234739A (zh) * | 2021-05-26 | 2021-08-10 | 云南中烟工业有限责任公司 | 烟草细胞色素p450亚家族cyp710a基因及应用 |
| CN118272399A (zh) * | 2024-04-19 | 2024-07-02 | 华中农业大学 | 一种应用OfCYP244基因提高桂花花瓣香气物质芳樟醇的方法 |
| CN118773150A (zh) * | 2024-06-03 | 2024-10-15 | 上海大学 | 一种银胶菊素c2羟化酶及其编码基因、制备方法与应用 |
| CN119876057A (zh) * | 2025-01-16 | 2025-04-25 | 南京师范大学 | Cyp71p2酶、生物材料及应用 |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10266836B2 (en) | 2001-11-13 | 2019-04-23 | U.S. Smokeless Tobacco Company Llc | Tobacco nicotine demethylase genomic clone and uses thereof |
| US7812227B2 (en) | 2001-11-13 | 2010-10-12 | U.S. Smokeless Tobacco Company | Cloning of cytochrome p450 genes from nicotiana |
| US8592663B2 (en) | 2001-11-13 | 2013-11-26 | U.S. Smokeless Tobacco Company Llc | Tobacco nicotine demethylase genomic clone and uses thereof |
| US7700851B2 (en) | 2001-11-13 | 2010-04-20 | U.S. Smokeless Tobacco Company | Tobacco nicotine demethylase genomic clone and uses thereof |
| US7700834B2 (en) | 2001-11-13 | 2010-04-20 | U.S. Smokless Tobacco Company | Nicotiana nucleic acid molecules and uses thereof |
| EP2330191A1 (en) | 2003-10-16 | 2011-06-08 | U.S. Smokeless Tobacco Company | Cloning of cytochrome P450 genes from nicotiana |
| JP4519137B2 (ja) * | 2003-10-16 | 2010-08-04 | ユーエス スモークレス タバコ カンパニー | タバコ属（Ｎｉｃｏｔｉａｎａ）からのチトクロムｐ４５０遺伝子のクローニング |
| US8586837B2 (en) | 2004-04-29 | 2013-11-19 | U.S. Smokeless Tobacco Company Llc | Nicotiana nucleic acid molecules and uses thereof |
| EP1851317B1 (en) | 2005-02-23 | 2011-10-26 | North Carolina State University | Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes |
| US8319011B2 (en) | 2006-12-15 | 2012-11-27 | U.S. Smokeless Tobacco Company Llc | Tobacco plants having reduced nicotine demethylase activity |
| US11332753B2 (en) | 2006-12-15 | 2022-05-17 | U.S. Smokeless Tobacco Company Llc | Tobacco plants having reduced nicotine demethylase activity |
| BRPI0820042B1 (pt) | 2007-11-12 | 2020-05-19 | North Carolina State University | método de obtenção de uma planta de tabaco, ou célula ou parte desta, tendo níveis reduzidos de nornicotina, produto de tabaco, método para fabricar um produto de tabaco, polinucleotídeo isolado, cassete de expressão, polipetpídeo isolado e método de obtenção de uma planta, ou parte de planta desta, do gênero nicotiana |
| PH12012501393B1 (en) | 2010-01-15 | 2017-10-25 | Univ North Carolina State | Compositions and methods for minimizing nornicotine synthesis in tobacco |
| KR20140034160A (ko) | 2011-02-28 | 2014-03-19 | 알트리아 클라이언트 서비시스 인코포레이티드 | 담배 근친교배 식물체 albex1f 및 albex1ms |
| BR112013021515A2 (pt) | 2011-02-28 | 2018-06-12 | Univ North Carolina State | plantas congênitas de tabaco ncbex1f, ncbex1ms e nc ex90 |
| WO2014110363A1 (en) | 2013-01-11 | 2014-07-17 | North Carolina State University | Tobacco inbred plants k326 src, cms k326 src, k346 src, cms k346 src, nc1562-1 src, nctg-61 src, cms nctg-61 src and hybrid nc196 src |
| EP2964016A1 (en) | 2013-03-05 | 2016-01-13 | North Carolina State University | Tobacco inbred and hybrid plants and uses thereof |
| WO2015051454A1 (en) * | 2013-10-07 | 2015-04-16 | Vineland Research And Innovation Centre | Compositions and methods for producing steviol and steviol glycosides |
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| EP3113603A1 (en) | 2014-03-03 | 2017-01-11 | North Carolina State University | Tobacco inbred and hybrid plants and tobacco products made thereof |
| EP3113602A1 (en) | 2014-03-03 | 2017-01-11 | North Carolina State University | Tobacco inbred and hybrid plants and tobacco products made thereof |
| US20240218386A1 (en) * | 2019-12-03 | 2024-07-04 | Altria Client Services Llc | Compositions and Methods for Producing Tobacco Plants and Products Having Altered Alkaloid Levels |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1152029A (zh) * | 1995-12-11 | 1997-06-18 | 浙江医科大学 | 人细胞色素p450系列转基因细胞株 |
| CN1341631A (zh) * | 2000-09-05 | 2002-03-27 | 上海博德基因开发有限公司 | 一种新的多肽——人细胞色素P450Cys亚铁血红素－铁蛋白9.13和编码这种多肽的多核苷酸 |
| CN1382707A (zh) * | 2001-04-26 | 2002-12-04 | 上海博德基因开发有限公司 | 一种多肽——细胞色素p－450p－2－48.84和编码这种多肽的多核苷酸 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2002244262B2 (en) * | 2001-03-09 | 2007-07-12 | University Of Kentucky Research Foundation | Cytochrome P450s and uses thereof |
| KR20040091124A (ko) * | 2002-03-12 | 2004-10-27 | 유.에스.스모크리스 토바코 컴퍼니 | 니코티아나 사이토크롬 ｐ450 유전자의 클로닝 |
| EP2336305A3 (en) * | 2002-10-16 | 2011-10-05 | U.S. Smokeless Tobacco Company LLC | Cloning of cytochrome P450 genes from Nicotiana |
-
2004
- 2004-10-15 CN CN2011102770337A patent/CN102321641A/zh active Pending
- 2004-10-15 AT AT04795252T patent/ATE536415T1/de active
- 2004-10-15 BR BRPI0415363-4A patent/BRPI0415363A/pt not_active Application Discontinuation
- 2004-10-15 ES ES04795252T patent/ES2377868T3/es not_active Expired - Lifetime
- 2004-10-15 EP EP04795252A patent/EP1675957B1/en not_active Expired - Lifetime
- 2004-10-15 JP JP2006535343A patent/JP5422099B2/ja not_active Expired - Lifetime
- 2004-10-15 WO PCT/US2004/034065 patent/WO2005038033A2/en not_active Ceased
- 2004-10-15 PL PL04795252T patent/PL1675957T3/pl unknown
- 2004-10-15 AU AU2004282576A patent/AU2004282576B2/en not_active Expired
-
2010
- 2010-04-28 AU AU2010201695A patent/AU2010201695B2/en not_active Expired
- 2010-04-28 AU AU2010201696A patent/AU2010201696B2/en not_active Expired
- 2010-07-26 JP JP2010166914A patent/JP5721357B2/ja not_active Expired - Lifetime
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1152029A (zh) * | 1995-12-11 | 1997-06-18 | 浙江医科大学 | 人细胞色素p450系列转基因细胞株 |
| CN1341631A (zh) * | 2000-09-05 | 2002-03-27 | 上海博德基因开发有限公司 | 一种新的多肽——人细胞色素P450Cys亚铁血红素－铁蛋白9.13和编码这种多肽的多核苷酸 |
| CN1382707A (zh) * | 2001-04-26 | 2002-12-04 | 上海博德基因开发有限公司 | 一种多肽——细胞色素p－450p－2－48.84和编码这种多肽的多核苷酸 |
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| CN103361368A (zh) * | 2012-03-27 | 2013-10-23 | 华中农业大学 | 一种棉花细胞色素p450基因及应用 |
| CN103361368B (zh) * | 2012-03-27 | 2015-03-04 | 华中农业大学 | 一种棉花细胞色素p450基因及应用 |
| CN102796821A (zh) * | 2012-08-30 | 2012-11-28 | 浙江中烟工业有限责任公司 | 一种检测烟草烟碱转化相对水平的方法 |
| CN110305893B (zh) * | 2018-03-23 | 2022-09-06 | 中国科学院分子植物科学卓越创新中心 | 棉酚生物合成途径基因cyp71be79及其应用 |
| CN110305893A (zh) * | 2018-03-23 | 2019-10-08 | 中国科学院上海生命科学研究院 | 棉酚生物合成途径因cyp71be79及其应用 |
| CN111849931A (zh) * | 2019-04-26 | 2020-10-30 | 西南大学 | 川桑色氨酸羟化酶t5h2及其应用 |
| CN110923249A (zh) * | 2019-10-15 | 2020-03-27 | 贵州省烟草科学研究院 | 烟草CyP71及在调控植物表皮毛发育方面的应用 |
| CN110885841A (zh) * | 2019-12-24 | 2020-03-17 | 河南科技大学 | 葡萄VyCYP89A2基因及其编码蛋白和基因在抗旱品种育种中的应用 |
| CN110885841B (zh) * | 2019-12-24 | 2022-04-12 | 河南科技大学 | 葡萄VyCYP89A2基因及其编码蛋白和基因在抗旱品种育种中的应用 |
| CN113234739A (zh) * | 2021-05-26 | 2021-08-10 | 云南中烟工业有限责任公司 | 烟草细胞色素p450亚家族cyp710a基因及应用 |
| CN113234739B (zh) * | 2021-05-26 | 2023-12-26 | 云南中烟工业有限责任公司 | 烟草细胞色素p450亚家族cyp710a基因及应用 |
| CN118272399A (zh) * | 2024-04-19 | 2024-07-02 | 华中农业大学 | 一种应用OfCYP244基因提高桂花花瓣香气物质芳樟醇的方法 |
| CN118773150A (zh) * | 2024-06-03 | 2024-10-15 | 上海大学 | 一种银胶菊素c2羟化酶及其编码基因、制备方法与应用 |
| CN119876057A (zh) * | 2025-01-16 | 2025-04-25 | 南京师范大学 | Cyp71p2酶、生物材料及应用 |
| CN119876057B (zh) * | 2025-01-16 | 2025-09-23 | 南京师范大学 | Cyp71p2酶、生物材料及应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| JP5422099B2 (ja) | 2014-02-19 |
| WO2005038033A2 (en) | 2005-04-28 |
| AU2010201695A1 (en) | 2010-05-20 |
| BRPI0415363A (pt) | 2006-12-12 |
| AU2010201696B2 (en) | 2011-10-20 |
| WO2005038033A8 (en) | 2005-07-14 |
| ES2377868T3 (es) | 2012-04-02 |
| JP2007508821A (ja) | 2007-04-12 |
| ATE536415T1 (de) | 2011-12-15 |
| AU2004282576A1 (en) | 2005-04-28 |
| EP1675957B1 (en) | 2011-12-07 |
| AU2004282576B2 (en) | 2010-01-28 |
| AU2010201695B2 (en) | 2012-06-28 |
| JP5721357B2 (ja) | 2015-05-20 |
| PL1675957T3 (pl) | 2012-06-29 |
| AU2010201696A1 (en) | 2010-05-20 |
| JP2010279377A (ja) | 2010-12-16 |
| EP1675957A1 (en) | 2006-07-05 |
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