CN102318586B - Pyemotidae production method - Google Patents
Pyemotidae production method Download PDFInfo
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- CN102318586B CN102318586B CN 201110234422 CN201110234422A CN102318586B CN 102318586 B CN102318586 B CN 102318586B CN 201110234422 CN201110234422 CN 201110234422 CN 201110234422 A CN201110234422 A CN 201110234422A CN 102318586 B CN102318586 B CN 102318586B
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Abstract
The invention discloses a pyemotidae production method, which solves the problems of difficult batch production of substitute hosts and the unmatched obtaining time and usage time in the existing natural enemy pyemotidae production. The pyemotidae production method adopts the following steps of: a, selecting the substitute hosts to unstick, wherein the substitute hosts are roe; b, refrigerating after laminating; c, selecting the intermediate hosts to proliferate the pyemotidae, wherein the intermediate hosts are the barley pest beginning hatchpupa; and d, inoculating the substitute hosts to cultivate. Through selecting, treating the substitute hosts and improving the inoculation and cultivation method and device, the problems of difficult batch production of the substitute hosts and the unmatched obtaining time and usage time in the existing natural enemy pyemotidae production are overcome.
Description
Technical field
The present invention relates to the raising technology of Pu mite, be specially the production method of a kind of Pu mite.
Background technology
Forestry and agriculture pest are caused serious harm to forestry ecotope and crops, all adopt chemical pesticide that above-mentioned insect is prevented and treated at present both at home and abroad, prevent and treat as not adopting chemical pesticide, the economy that the forestry ecotope is caused the annual pest of China and ecological with a toll of 88,000,000,000 yuan, social service function, bio-diversity etc. are lost above 5,000 hundred million yuan; The direct economic loss that crops are caused is up to 80,000,000,000 yuan, and worldwide the loss that crop is caused then can be above 1,000 trillion dollars.A large amount of chemical pesticides that use have caused serious pollution and destruction to ecotope, make the environmental hormone problem serious day by day, food-safety problem is outstanding day by day, the production of chemical pesticide has simultaneously also consumed a large amount of energy, CO2 emissions is increased, discharged a large amount of poisonous and harmful substances simultaneously.
Utilize natural enemy Pu mite that pest is prevented and treated to have with worm and control worm, biotic population internal balance, free from environmental pollution, the characteristics that do not develop immunity to drugs, can control for a long time continually and steadily disaster, it is the general orientation of world's biological control, upward natural enemy Pu mite range of application is very limited but produce at present, one of them major reason is that the alternative host that the Pu mite breeds originates limited, be subjected to effect of natural conditions big, be difficult to acquisition in enormous quantities, often be difficult to coincide the service time that obtains the time simultaneously and produce, and limited the application of natural enemy on producing greatly.
Summary of the invention
The present invention to substitute the host and is difficult to obtain in batches in order to exist in the production that solves existing natural enemy Pu mite, and the problem that misfits acquisition time and service time, and the production method of a kind of Pu mite is provided.
The present invention adopts following technical scheme to realize: the production method of Pu mite, and adopt following steps: a, choose alternative host's unsticking, described alternative host is fish-egg; Refrigerate behind b, the overlay film; C, choose vector propagation Pu mite, described vector is that the barley worm just incubates pupa; D, access substitute the host and cultivate.Substitute choosing and the unsticking overlay film of host's fish-egg, draw by test of many times, fish-egg can obtain in batches, adopts the barley worm just to incubate pupa simultaneously and also draws through test of many times for parasitic Pu mite.
Choose the method for vector propagation kind of Pu, dechlorination cleans after employing following steps: a, the vector thinning; B, put into the inoculation of incubator and Pu mite; C, incubator is placed on glass greenhouse endoparasitism propagation, vector adopts the barley worm just to incubate pupa, and As time goes on exocuticle can progressively harden, and influence Pu mite parasitic rate, barley worm pupa exocuticle is carried out the thinning processing can improve the parasitic rate of Pu mite.
Insert to substitute and put it in the paper bag of upper end open before the host cultivates.
Access substitutes and puts it in the housing of two capsule shape before the host cultivates, and have the hole on the housing, and two housings one end connects by connecting rod.The capsule shape housing can be manufactured with the medical capsule material, and also available paper material is made, and two housings can connect by connecting rod, also can be by connections such as cotton ropes.
Before the alternative host of access cultivates two alternative hosts are bonded at the two ends of cotton rope, the length of cotton rope is the 2-30 millimeter, is mainly used in the vegetables control, and the length of cord is chosen according to the difference of control worm kind.
The fish-egg unsticking is to adopt following steps: talcum powder and sodium chloride mix soluble in water, stir into suspension, and water temperature is controlled at 3-5 ℃, fish-egg is poured into stirred the back filtration in the suspension.
The fish-egg overlay film adopts following steps: clear water is heated to 60-80 ℃, agar is poured in the water stir, and dries in the air to 10-20 ℃, beating eggs is stirred evenly to pour in the clear water stir, and the weight percent of clear water, agar and egg is 8:1:1.
Substituting the host is frog's egg.
A kind of for the production method of implementing the Pu mite custom-designed incubator, comprise that the equal opening of upper and lower side and arbitrary sidewall are the casing of door-plate, lower box is provided with humidifier, auxiliary blower, sterilamp and effective accumulated temperature control device, casing top is located at some inoculation dishes that distribute up and down, each inoculation dish comprises three branch dishes placing up and down, three branch dishes include highly identical housing, be provided with the iron wire inside casing that is positioned at its middle height in two housings of upside, the insect receiving net that is positioned at its below and highly equals the housing height is fixedly arranged on the iron wire inside casing, between iron wire inside casing and the housing corresponding with it quarter butt is arranged fixedly, scribble separation layer on the quarter butt, vector is put into the insect receiving net bottom of middle level branch dish, and then the Pu mite is inserted parasitic, treat parasitism finish after in the branch dish of upper strata inoculation substitute host's fish-egg and cultivate, because the structural design of incubator, the upper strata contacts with the insect receiving net of middle level branch dish, but all separate with adjacent inoculation dish, separation layer on the quarter butt prevents that adult from escaping to housing, strengthen air by auxiliary blower in the incubation and flow, prevent Signs of Mould; Increase humidity by humidifier; Sterilize by ultraviolet disinfection lamp; Development time by effective accumulated temperature control device control Pu mite matches natural enemy production time and control of insect time, improves natural enemy and prevents and treats efficient.
A kind of for the production method of implementing the Pu mite custom-designed glass greenhouse, it is characterized in that: glass greenhouse comprises the greenhouse body, the greenhouse bodies top is the blinds roof, the blinds roof is provided with solar panel, between the high humidity that is provided with in the greenhouse body that north and south arranges and separates by glass partition, in wet between and between low temperature, have glass door on the glass partition, be provided with the refrigerating box that is connected in series with solar panel between low temperature, and the north side outer wall is provided with heat-insulation layer between low temperature.During operation, Pu mite behind alternative host's fish-egg and the parasitism is cultivated and vector barley worm just hatches and the parasitism of Pu mite all carries out in glass greenhouse, in being placed between high humidity after the inoculation, between wet in being placed on after 24 hours, put into after cultivation is finished between low temperature, can directly refrigerate between low temperature winter, and the refrigerating box of putting into summer between low temperature is preserved, refrigerating box is powered by solar panel, and energy consumption is lower.
The present invention by to substitute the host choose, handle and to the improvement of inoculation, cultured method and device, overcome to exist in the production of existing natural enemy Pu mite and substituted the host and be difficult to obtain in batches, and acquisition time and service time the problem that misfits, substitute host's wide material sources, with low cost, can be used for batch production large-scale production natural enemy; Adopt the effective accumulated temperature control technology, natural enemy production time and PCO optimum period are fitted like a glove, increased substantially the efficient of preventing and treating of natural enemy; Adopt the hot-house culture method, automatic sterilization humidification, automatic gas self-loopa can effectively reduce energy resource consumption; Reduce the usage amount of chemical pesticide significantly, reduce the energy resource consumption of pesticide producing, reduce CO2 emissions, meet the industrial policy of national green economy, environmental protection and economy, low-carbon economy and food security.
Description of drawings
Fig. 1 is the structural representation of incubator among the present invention;
Fig. 2 is the structural representation of upside branch dish among Fig. 1;
Fig. 3 is the schematic top plan view of Fig. 2;
Fig. 4 is the structural representation of glass greenhouse among the present invention.
Among the figure: 1-casing, 2-humidifier, 3-auxiliary blower, 4-inoculation dish, 5-branch dish, 6-housing, 7-iron wire inside casing, the 8-insect receiving net, the 9-quarter butt, 10-greenhouse body, the 11-glass partition is between the 12-high humidity, between wet among the 13-, between 14-low temperature, 15-glass door, 16-refrigerating box, 17-heat-insulation layer.
Embodiment
The production method of Pu mite adopts following steps: a, chooses alternative host's unsticking, and described alternative host is fish-egg; Refrigerate behind b, the overlay film; C, choose vector propagation Pu mite, described vector is that the barley worm just incubates pupa; D, access substitute the host and cultivate.
During concrete enforcement, buy the maturation raun to be produced of the bigger fingerling of fish-egg, as black carp, grass carp, catfish, loach etc., put into about 60 centimetres an of diameter, high about 40 centimetres container, pour a certain amount of clear water in the container into, water temperature is controlled at the 3-5 degree, raun is taken out, fish belly portion left by plane, and the place's of getting fish-egg is put into clear water, rinsing 3-5 minute; The above-mentioned fish-egg of making is put into refrigerating box refrigerate, temperature is controlled between the 1-3 degree, can preserve for a long time.
Choose the method for vector propagation kind of Pu, dechlorination cleans after employing following steps: a, the vector thinning; B, put into the inoculation of incubator and Pu mite; C, incubator is placed on glass greenhouse endoparasitism propagation.
During concrete enforcement, by vector Pu mite population is enlarged rapidly, reach the purpose of fast breeding natural enemy, vector is that the barley worm just incubates pupa, commercial or control oneself and breed, getting the mass percent example is that 10 parts of sodium carbonate and 90 parts of bleaching powder (calcium hypochlorite) add 40 parts of clear water, mixes, and above-mentioned thinning liquid is heated to the 10-14 degree, the barley worm is just incubated pupa put into thinning liquid, soaked 3-5 minute, and removed thinning liquid, the barley worm after the thinning is just incubated thio-acid sodium solution dechlorination 3-5 minute that pupa is put into 1-2%, take out, barley worm behind the dechlorination is just incubated pupa be placed in the clear water rinsing 3-5 minute, pull out, get final product with extension set is air-dry; Vector barley worm is after treatment just incubated the pupa taking-up, Pu mite kind mite is inserted again, Pu mite kind mite is bought inoculative proportion from market: a kind mite breeds body (each kind mite breeds body and contains 100,000 Pu mites approximately) 10-20 barley worm of inoculation and just incubates pupa.
Insert to substitute and put it in the paper bag of upper end open before the host cultivates.Be used for the small size insect is prevented and treated.
Access substitutes and puts it in the housing of two capsule shape before the host cultivates, and have the hole on the housing, and two housings one end connects by connecting rod.Be used for the large tracts of land insect is prevented and treated.
Before the alternative host of access cultivates two alternative hosts are bonded at the two ends of cotton rope, the length of cotton rope is the 2-30 millimeter, is used for the control to insects such as vegetables.
The fish-egg unsticking is to adopt following steps: talcum powder and sodium chloride mix soluble in water, stir into suspension, and water temperature is controlled at 3-5 ℃, fish-egg is poured into stirred the back filtration in the suspension.
During concrete enforcement, get 80 gram talcum powder (magnesium silicate) and 20 gram sodium chloride mixing, be dissolved in the 10L clear water, stir into suspension, water temperature is controlled at the 3-5 degree, every 10L suspension can take off 1-5 kilogram fish-egg, fish-egg after tentatively cleaning is poured in the suspension, slowly stirred, the suspension that will contain fish-egg is then poured mesh into than in the little sieve of fish-egg, filter out suspension, to the continuous unsticking of viscosity fish-egg 3-5 time, weak viscosity fish-egg unsticking 1 time, unsticking fully just, will through unsticking fish-egg put into clean container, film covering solution is slowly poured in the container, fully stirred, make fish-egg fully contact solution, and the container bottom no liquid is oozed out, dry up fish-egg with hair-dryer again, stir while blow, until the fish-egg drying, add millesimal talcum powder again and fully stir, make fully to separate between the fish-egg to get final product.
The fish-egg overlay film adopts following steps: clear water is heated to 60-80 ℃, agar is poured in the water stir, and dries in the air to 10-20 ℃, beating eggs is stirred evenly in the pouring clear water stir, and the weight percent of clear water, agar and egg is 8:1:1.
Substituting the host is frog's egg.During enforcement, collect the ovum that wood frog, the stone frog have just given birth to, in container rinsing 3-5 minute.
A kind of for the production method of implementing the Pu mite custom-designed incubator, comprise that the equal opening of upper and lower side and arbitrary sidewall are the casing 1 of door-plate, casing 1 bottom is provided with humidifier 2, auxiliary blower 3, sterilamp and effective accumulated temperature control device, casing 1 top is located at some inoculation dishes 4 that distribute up and down, each inoculation dish 4 comprises three branch dishes 5, three branch dishes 5 include highly identical housing 6, be provided with the iron wire inside casing 7 that is positioned at its middle height in two housings 6 of upside, the insect receiving net 8 that is positioned at its below and highly equals housing 6 height is fixedly arranged on the iron wire inside casing 7, between iron wire inside casing 7 and the housing 6 corresponding with it quarter butt 9 is arranged fixedly, scribble separation layer on the quarter butt 9.
During concrete enforcement, the high 2-3 rice of incubator, do for the stainless steel and iron leather all around, the front is chamber door, and 2-3 centimetre of wide baffle plate respectively established on the inner both sides of incubator, the incubator bottom is equipped with four wheels, be convenient to move, be provided with a ultraviolet disinfecting in the incubator from top to bottom every about 50 centimetres, sterilamp is controlled automatically, opened sterilization one time when slight in 10 minutes to 2 for every, the time is 10-30 minute; The effective accumulated temperature control device can be measured real time temperature, and the natural enemy threshold of development is set, and calculating natural enemy effective accumulated temperature also shows:
Natural enemy effective accumulated temperature=(real time temperature-natural enemy threshold of development) * time (hour or day)
If numerical value is positive number, then be significant figure, if numerical value is negative or zero, then be significant figure, when calculating effective accumulated temperature, remove.The effective accumulated temperature control device is made up of temp probe and single-chip microcomputer.
A kind of for the production method of implementing the Pu mite custom-designed glass greenhouse, glass greenhouse comprises greenhouse body 10, body 10 tops in greenhouse are the blinds roof, the blinds roof is provided with solar panel, between the high humidity that is provided with in the greenhouse body 10 that north and south arranges and separates by glass partition 11 12, in wet between 13 and low temperature between 14, have glass door 15 on the glass partition 11, be provided with the refrigerating box 16 that is connected in series with solar panel in 14 between low temperature, and 14 north side outer walls are provided with heat-insulation layer 17 between low temperature.
During concrete enforcement, the incubator that connects kind is put between high humidity 12, cultivation temperature is controlled between 24-30 ℃, humidity is 95-100%, cultivate and made the Pu mite fully parasitic to the vector body in 24 hours, when beginning is parasitic, namely open the effective accumulated temperature control device on the incubator, Pu mite threshold of development is set opening entry effective accumulated temperature; The inoculation of Pu mite is after 24 hours, after treating the abundant parasitism of Pu mite, with incubator from 12 taking out between high humidity, put into and 13 continue cultivations between wet, temperature control is between 12-30 ℃, humidity is between the 50-80%, finishes to insert after grow producing filial generation Pu mite substituting the host up to the Pu mite, successively between high humidity 12 and in wet between 13 equal conditions be cultured to and breed natural enemy Pu mite in batches.
Claims (8)
1. the production method of a Pu mite is characterized in that: adopt following steps: a, choose and substitute host's unsticking, described alternative host is fish-egg; Refrigerate behind b, the overlay film; C, choose vector propagation Pu mite, described vector is that the barley worm just incubates pupa; D, access substitute the host and cultivate.
2. the production method of Pu mite according to claim 1 is characterized in that: choose the method for vector propagation kind of Pu, dechlorination cleaning after employing following steps: a, the vector thinning; B, put into the inoculation of incubator and Pu mite; C, incubator is placed on glass greenhouse endoparasitism propagation.
3. the production method of Pu mite according to claim 1 and 2 is characterized in that: put it in the paper bag of upper end open before the alternative host of access cultivates.
4. the production method of Pu mite according to claim 1 and 2 is characterized in that: put it in the housing of two capsule shape before the alternative host of access cultivates, have the hole on the housing, and two housings one end connects by connecting rod.
5. the production method of Pu mite according to claim 1 and 2 is characterized in that: insert the two ends that before substituting the host and cultivating two alternative hosts are bonded at cotton rope, the length of cotton rope is the 2-30 millimeter.
6. the production method of Pu mite according to claim 1 and 2 is characterized in that: the fish-egg unsticking is to adopt following steps: talcum powder and sodium chloride mix soluble in water, stir into suspension, and water temperature control at 3-5 ℃, fish-egg is poured into stirred the back filtration in the suspension.
7. the production method of Pu mite according to claim 1 and 2, it is characterized in that: the fish-egg overlay film adopts following steps: clear water is heated to 60-80 ℃, agar poured in the water stir, dry in the air to 10-20 ℃, beating eggs stirred evenly to pour in the clear water stir, the weight percent of clear water, agar and egg is 8:1:1.
8. the production method of Pu mite according to claim 1 and 2 is characterized in that: substitute the host and be frog's egg.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201110234422 CN102318586B (en) | 2011-08-16 | 2011-08-16 | Pyemotidae production method |
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| CN 201110234422 CN102318586B (en) | 2011-08-16 | 2011-08-16 | Pyemotidae production method |
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| CN1224316C (en) * | 2002-02-24 | 2005-10-26 | 张艳璇 | Artificial raising method of cuculber amblyseius predatory mite |
| CN1184880C (en) * | 2002-07-31 | 2005-01-19 | 北京市植物园 | Method for scaled breeding cattail mite and application in biological preventing and controlling pest-insect |
| CN100388884C (en) * | 2005-09-23 | 2008-05-21 | 段东红 | Method for breeding pyemote, and its culturing vessel, and method for releasing same |
| WO2010096821A2 (en) * | 2009-02-23 | 2010-08-26 | Cell & Tissue Systems, Inc. | Method for ice-free cryopreservation of tissue |
| CN101569297B (en) * | 2009-06-08 | 2011-11-16 | 河北省农林科学院昌黎果树研究所 | Breeding method of pyemotes zhonghuajia and application thereof in pest biological control |
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Owner name: TAIYUAN MEILIN AGRICULTURAL TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: DUAN DONGHONG Effective date: 20130307 |
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