CN102283320A - Alginate feed additive and alginate feed - Google Patents
Alginate feed additive and alginate feed Download PDFInfo
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- CN102283320A CN102283320A CN2011102088668A CN201110208866A CN102283320A CN 102283320 A CN102283320 A CN 102283320A CN 2011102088668 A CN2011102088668 A CN 2011102088668A CN 201110208866 A CN201110208866 A CN 201110208866A CN 102283320 A CN102283320 A CN 102283320A
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- alginates
- feed
- feed addictive
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- azotobacter vinelandii
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses an alginate feed additive and an alginate feed. The feed additive contains alginate; and the alginate feed contains 6 to 9 weight percent of alginate feed additive. In the invention, the alginate used as the feed additive is applied to the feed, provides rich bifidobacterium growth factors for the growth of animals, has a remarkable effect of increasing weight during the growth of the animals, and is safe and reliable; and the alginate obtained through bacterial culture ensures that the production cost is greatly reduced, can be widely applied to feeds, and reduces the raising cost of livestock and poultry.
Description
Technical field
The present invention relates to field of feed, particularly relate to a kind of alginates feed addictive and alginates feed.
Background technology
The seventies in 20th century, it is a kind of positive growth factor for bifidus that Tokyo Univ Japan's light is not taught the discovery feature compound sugar, can promote beneficial bacterium fast breedings such as the interior Bifidobacterium of animal intestinal especially, is of value to humans and animals health.Algae glycan function of dominant compound sugar refines acquisition usually, but needs a large amount of raw materials by the algae glycan that refines in the natural marine brown alga from the natural marine brown alga, cause cost to rise, so change by bacterium production wide development potentiality is arranged.Have similar function and effect (Rehm, B.H.A., and S.Valla.1997 by bacteriogenic algae glycan (claiming alginates again) and the algae glycan that from the natural marine brown alga, extracts; Uwe Remminghorst and Bernd H. A.Rehm, 2006), can make purposes such as surgical dressing, tooth mould, food and medicinal slow release agent, or as immobilised enzymes and cell engineering aspect.
Feed addictive is meant a small amount of or micro substance that adds in production of fodder processing, use, in feed consumption seldom but effect significantly.Feed addictive is the raw material that modern feed industry must use, and to strengthening the basal feed nutritive value, improves breeding performonce fo animals, guarantees animal health, saves feed cost, and improving aspect such as livestock products quality has obvious effects.But alginates yet there are no in feed and use as a kind of positive growth factor for bifidus.
Summary of the invention
Technical problem to be solved by this invention is that alginates are applied to feed, and a kind of alginates feed addictive and alginates feed are provided, and this feed has tangible effect of gain to domestic animal.
In order to solve the problems of the technologies described above, the present invention adopts following technical scheme:
Contain alginates in the alginates feed addictive of the present invention.In feed addictive prescription commonly used, add alginates, make it have tangible effect of gain animal.
The preferred azotobacter vinelandii alginates of alginates in the above-mentioned feed addictive.Azotobacter vinelandii (Azotobacter vinelandii) belongs to azotobacter and belongs to, to this bacterium of the many uses of research (particularly azotase biology) of biological nitrogen fixation.The azotobacter vinelandii alginates are cultivated to extract to separate by azotobacter vinelandii and are obtained.
Concrete, the quality percentage composition of azotobacter vinelandii alginates is 1～2.5% in the aforementioned alginates feed addictive.
Best, the quality percentage composition of azotobacter vinelandii alginates is 1.5% in the aforementioned alginates feed addictive.
The present invention also provides two kinds of preparation methods of azotobacter vinelandii alginates used in the aforementioned alginates feed addictive:
Method one: get azotobacter vinelandii bacterium liquid, on no nitrogen fluid nutrient medium, cultivated 4 days, then culture evenly is applied in the plating medium and cultivated 2-3 days, scrape and get all cultures, with physiological saline washing, centrifugal, abandoning supernatant, precipitate with 4 ℃ of isopropyl alcohol extractings, the extracting residue washs with physiological saline, and freeze drying promptly gets the azotobacter vinelandii alginates.
Method two: get azotobacter vinelandii bacterium liquid, on no nitrogen fluid nutrient medium, cultivated 4 days, culture evenly is applied to cultivates 2～3 days in the plating medium then, scrape and get all cultures, add water, 80 ℃ of water-bath 8h are centrifugal, the gained supernatant concentration, concentrate adds 3 times of amount 95% ethanol, spends the night, and abandons supernatant, centrifugation, the vacuum drying of gained precipitation promptly gets the azotobacter vinelandii alginates.
Alginates feed of the present invention contains 6～9%(weight in described feed) the alginates feed addictive.In poultry commonly used, feed stripped prescription, add the alginates feed addictive, can promote beneficial bacterium fast breedings such as the interior Bifidobacterium of animal intestinal, guarantee the weight of animals to be increased animal health fast.
Preferably, contain 7.5%(weight in the above-mentioned alginates feed) the alginates feed addictive.
Concrete, according to listed as parts by weight, aforementioned alginates feed is to be mixed by 45～65 parts of corn flours, 20～25 parts in wheat bran, 7～13 parts of analysis for soybean powder, 5～9 parts of oil cakes, 1～2 part of bone meal, 0.3～0.7 part in calcium phosphate, 0.3～0.7 part of salt and alginates feed addictive.
Best, aforementioned alginates feed is to be mixed by 50 parts of corn flours, 23 parts in wheat bran, 10 parts of analysis for soybean powder, 7 parts of oil cakes, 1.5 parts of bone meal, 0.5 part in calcium phosphate, 0.5 part of salt and alginates feed addictive.
Compared with prior art, the present invention is applied to alginates in the feed as feed addictive, for the growth of animal provides the abundant bifidobacterium growth factor, growth to animal has tangible effect of gain, and it is safe and reliable, use reduces production cost by the alginates that Bacteria Culture obtains greatly, can be widely used in feed, reduces the feeding cost of domestic animal, poultry.
The specific embodiment
Embodiment 1:
(1) preparation of azotobacter vinelandii alginates: do not have at 20ml and to add 1 μ l azotobacter vinelandii (Azotobacter vinelandii) in the nitrogen fluid nutrient medium, 26-30 ℃ of shaking table cultivated 4 days, then the 1.5ml culture evenly is coated with and is transplanted in the plating medium, cultivated 2-3 days for insulating box 26-30 ℃, 20ml bacterium liquid can be coated with 13 medium size culture dishes.Scrape and get all cultures, with the physiological saline washing, then with washing with physiological saline after 4 ℃ of isopropyl alcohol extractings, centrifugal ,-20～-80 ℃ of freeze dryings promptly get the azotobacter vinelandii alginates, gained alginates average product is 0.1026 g/gCDM, and promptly every gram cell dry contains 0.1126 gram alginates.
(2) preparation alginates feed addictives (referring to additive here is not feed): 10000 milligrams of lysines, 10000 milligrams of methionine, selenium 12.5-25 milligram, vitamin A 6,000,000 IU, vitamin D
36,000,000 IU, 200 milligrams of antioxidants (ethoxyquinoline) mix, and the azotobacter vinelandii alginates that add step (1) make its content reach 1.5%.
(3) preparation alginates feed: corn flour 50kg, wheat bran 23kg, analysis for soybean powder 10kg, oil cake 7kg, bone meal 1.5kg, calcium phosphate 0.5kg, salt 0.5kg, alginates feed addictive 7.5kg, mix to mix thoroughly getting final product.
Adopt above-mentioned alginates feed, carry out following research:
1, alginates feed safety and to the influence of rabbit weightening finish
20 of 30 age in days rabbit are divided into two groups and carry out alginates safety test and growth performance determination test, and experimental period is 40 days.
Feed scarce alginates feed (corn flour 50kg, wheat bran 23kg, analysis for soybean powder 10kg, oil cake 7kg, bone meal 1.5kg, calcium phosphate 0.5kg, salt 0.5kg) the last week on-test, free choice feeding, three times on the one, every group of each 200 grams, water fountain is freely drunk water.
Since second week, experimental group is thrown in the alginates feed.Weigh weekly once, divide morning, noon and afternoon three feedings.Control group feeds intake routinely.Experimental group feeding method: get 0.4～1 kilogram of alginates feed stirring high-quality green grass and feed for 1～2 kilogram.Sooner or later observe rabbit behavior sign every day, and record.Carry out slaughter experiment after 40 days.By DIFFERENT FEED rabbit raising gaining effect being carried out the t check shows: t=4.476, experimental group and control group gaining effect difference is (t ﹥ t extremely significantly
0.01, P ﹤ 0.01), as table 1.
Table 1 alginates feed safety and to rabbit weight increment test result
2, the alginates feed is to the influence of goat growth performance
Animal experiment: select 30 of the local black goats of 60 ages in days.Experimental period is 75 days.30 goats are divided into three groups and carry out the growth performance determination test.
Feed scarce alginates feed the last week on-test, free choice feeding, every group every 300 gram, a twice-daily is freely drunk water.
Since second week, experiment I group feeding alginates feed, experiment II group feeding conventional feed (changing the alginates additive in the alginates feed into brown sugar), control group are fed routinely and are lacked the alginates feed.Experimental period,, every feed intake progressively was increased to 1200 grams by 400 gram feeds, and mixed high-quality green grass 0.4-2.0 kilogram by 0.2-0.6 kilogram feed and feed.Per two weeks weigh once.Morning, noon and afternoon secondary feeding is observed goat behavior sign every day sooner or later, and record.Carry out slaughter experiment after 75 days.By DIFFERENT FEED goat is raised weightening finish ANOVA showed significant: F=3.41 experiment as a result I group, experiment II group, gaining effect there were significant differences (F between control group
(0.05 2,27)﹤ F ﹤ F
(0.01 2,27)).
Table 2 alginates feed is to the result that influences of goat growth performance
Every average performance analysis every day of table 3 alginates feed addictive feed
Annotate: sheep valency and feed price are the there and then market price, 22 yuan/kg of sheep live-weight, and 2.00 yuan/kg of basal diet, the feed cost of test I, II, control group is respectively 0.98,0.94,0.90 yuan/kg, 0.30 yuan/kg of greenfeed.
3, the extend trial of hybridization goat alginates feed
Select 28 of the assorted goats of sheep raising family construction of Guizhou black goat, the big ear sheep in Jianyang, numb sheep and ripple, take house to herd associated methods and raise.Experimental period is 90 days.Weighed once in per during this time 30 days.Early, late secondary feeding, observe goat behavior sign every day sooner or later, and record.Feed feed the last week on-test, free choice feeding, every group every 300 gram, a twice-daily is freely drunk water.After second week, the every kilogram of feed of experiment I group adds the feed addictive that contains 0.15% alginates, and experimental period, every feed intake progressively was increased to 1200 grams by 400 grams.Feed every of every day high-quality green grass 0.4-2.0 kilogram in addition.Result such as table 4:
Table 4
4, the extend trial of azotobacter vinelandii alginates feed
Take house to herd associated methods respectively raises surplus the goat 1000 at sheep raising base, Guizhou, sheep raising family.During the extend trial, 2-5 monthly age goat average daily gain amount increases, and black goat average daily gain amount restrain/is only brought up to 70-85 gram/only by 60-70, and hybridization goat average daily gain amount restrain/only brings up to 80-120 by 60-80 and restrains/.Mountain forest, hillside vegetation coverage improve 10%.Sheep raising family annual income improves 2000-25000 unit.
Embodiment 2:
(1) preparation of azotobacter vinelandii alginates: do not have at 20ml and to add 1 μ l azotobacter vinelandii (Azotobacter vinelandii) in the nitrogen fluid nutrient medium, 26-30 ℃ of shaking table cultivated 4 days, then the 1.5ml culture evenly is coated with and is transplanted in the plating medium, cultivated 2-3 days for insulating box 26-30 ℃, 20ml bacterium liquid can be coated with 13 medium size culture dishes.Add water 2000ml respectively, 80 ℃ of water-bath 8h, centrifugal 10min gained supernatant concentration to 1/4 volume, concentrate adds 3 times of amount 95% ethanol, spends the night, and abandons supernatant, the centrifugal 10min of 3000rpm, the vacuum drying of gained precipitation promptly gets the azotobacter vinelandii alginates, and the alginates average product is 0.1089g/gCDM, and promptly every gram cell dry contains 0.1089 gram alginates.
(2) preparation alginates feed addictive: 10000 milligrams of lysines, 10000 milligrams of methionine, selenium 12.5-25 milligram, vitamin A 6,000,000 IU, vitamin D
36,000,000 IU, 200 milligrams of antioxidants (ethoxyquinoline) mix, and the azotobacter vinelandii alginates that add step (1) make its content reach 1%.
(3) preparation alginates feed: corn flour 51kg, wheat bran 20kg, analysis for soybean powder 13kg, oil cake 5kg, bone meal 2kg, calcium phosphate 0.3kg, salt 0.7kg, alginates feed addictive 8kg, mix to mix thoroughly getting final product.
Embodiment 3: adopt the method for embodiment 1 to prepare the azotobacter vinelandii alginates, in the normal diet additive, add the azotobacter vinelandii alginates make its quality percentage composition be 2.5% the alginates feed addictive, in common conventional feed prescription, add the alginates feed addictive make its quality percentage composition be 6% the alginates feed.
Embodiment 4: in the normal diet additive, add marine alga alginates (Seed weed) make its quality percentage composition be 1% the alginates feed addictive, adding the alginates feed addictive in common conventional feed prescription, to make its quality percentage composition be 9% alginates feed.
Claims (10)
1. an alginates feed addictive is characterized in that: contain alginates in the described feed addictive.
2. according to the described alginates feed addictive of claim 1, it is characterized in that: described alginates are azotobacter vinelandii alginates.
3. according to the described alginates feed addictive of claim 2, it is characterized in that: the quality percentage composition of described azotobacter vinelandii alginates in feed addictive is 1～2.5%.
4. according to the described alginates feed addictive of claim 3, it is characterized in that: the quality percentage composition of described azotobacter vinelandii alginates in feed addictive is 1.5%.
5. according to the arbitrary described alginates feed addictive of claim 2 to 4, it is characterized in that: described azotobacter vinelandii alginates are to be prepared from as follows:
Get azotobacter vinelandii bacterium liquid, on no nitrogen fluid nutrient medium, cultivated 4 days, then culture evenly is applied in the plating medium and cultivated 2-3 days, scrape and get all cultures, with physiological saline washing, centrifugal, abandoning supernatant, precipitate with 4 ℃ of isopropyl alcohol extractings, the extracting residue washs with physiological saline, and freeze drying promptly gets the azotobacter vinelandii alginates.
6. according to the arbitrary described alginates feed addictive of claim 2 to 4, it is characterized in that: described azotobacter vinelandii alginates are to be prepared from as follows:
Get azotobacter vinelandii bacterium liquid, on no nitrogen fluid nutrient medium, cultivated 4 days, culture evenly is applied to cultivates 2～3 days in the plating medium then, scrape and get all cultures, add water, 80 ℃ of water-bath 8h are centrifugal, the gained supernatant concentration, concentrate adds 3 times of amount 95% ethanol, spends the night, and abandons supernatant, centrifugation, the vacuum drying of gained precipitation promptly gets the azotobacter vinelandii alginates.
7. an alginates feed is characterized in that: contain 6～9%(weight in described feed) the alginates feed addictive.
8. according to the described alginates feed of claim 7, it is characterized in that: in described feed, contain 7.5%(weight) the alginates feed addictive.
9. according to claim 7 or 8 arbitrary described alginates feeds, it is characterized in that: according to listed as parts by weight, it is to be mixed by 45～65 parts of corn flours, 20～25 parts in wheat bran, 7～13 parts of analysis for soybean powder, 5～9 parts of oil cakes, 1～2 part of bone meal, 0.3～0.7 part in calcium phosphate, 0.3～0.7 part of salt and alginates feed addictive.
10. according to the described alginates feed of claim 9, it is characterized in that: it is to be mixed by 50 parts of corn flours, 23 parts in wheat bran, 10 parts of analysis for soybean powder, 7 parts of oil cakes, 1.5 parts of bone meal, 0.5 part in calcium phosphate, 0.5 part of salt and alginates feed addictive.
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| CN2011102088668A CN102283320A (en) | 2011-07-25 | 2011-07-25 | Alginate feed additive and alginate feed |
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| CN2011102088668A CN102283320A (en) | 2011-07-25 | 2011-07-25 | Alginate feed additive and alginate feed |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106011197A (en) * | 2016-05-20 | 2016-10-12 | 浙江省农业科学院 | Microorganism-derived algin and preparation and application thereof |
| CN113307697A (en) * | 2021-06-10 | 2021-08-27 | 贵州省畜牧兽医研究所 | Alginate organic fertilizer for livestock and poultry manure and preparation and application thereof |
| CN114747688A (en) * | 2022-04-19 | 2022-07-15 | 深圳市豆柴宠物用品有限公司 | 'four-in-one' combined additive capable of improving intestinal functions of dogs and cats and preparation method thereof |
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| CN101744145A (en) * | 2008-12-19 | 2010-06-23 | 中国科学院海洋研究所 | Meat quality flavor modifying agent for grass carp or carp and preparation method thereof |
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-
2011
- 2011-07-25 CN CN2011102088668A patent/CN102283320A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04126042A (en) * | 1990-09-14 | 1992-04-27 | Kimitsu Kagaku Kenkyusho:Kk | Feed composition of snails and sea urchins of marine product |
| CN1582707A (en) * | 2003-08-18 | 2005-02-23 | 汤珍山 | Selenium containing microopganic additive for forage and its preparation |
| CN101461454A (en) * | 2007-12-19 | 2009-06-24 | 烟台海岸带可持续发展研究所 | Non-hormone feed addictive and preparation method thereof |
| CN101744145A (en) * | 2008-12-19 | 2010-06-23 | 中国科学院海洋研究所 | Meat quality flavor modifying agent for grass carp or carp and preparation method thereof |
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Non-Patent Citations (1)
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106011197A (en) * | 2016-05-20 | 2016-10-12 | 浙江省农业科学院 | Microorganism-derived algin and preparation and application thereof |
| CN113307697A (en) * | 2021-06-10 | 2021-08-27 | 贵州省畜牧兽医研究所 | Alginate organic fertilizer for livestock and poultry manure and preparation and application thereof |
| CN114747688A (en) * | 2022-04-19 | 2022-07-15 | 深圳市豆柴宠物用品有限公司 | 'four-in-one' combined additive capable of improving intestinal functions of dogs and cats and preparation method thereof |
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Application publication date: 20111221 |