CN102283009A - Culture medium used for culturing cordyceps sinensis, artificial culturing method of cordyceps sinensis and cordyceps sinensis product - Google Patents
Culture medium used for culturing cordyceps sinensis, artificial culturing method of cordyceps sinensis and cordyceps sinensis product Download PDFInfo
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- CN102283009A CN102283009A CN 201010203872 CN201010203872A CN102283009A CN 102283009 A CN102283009 A CN 102283009A CN 201010203872 CN201010203872 CN 201010203872 CN 201010203872 A CN201010203872 A CN 201010203872A CN 102283009 A CN102283009 A CN 102283009A
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Abstract
The invention provides a culture medium used for culturing cordyceps sinensis, which comprises 1000 parts by weight of water, 500 to 1500 parts by weight of starch material, 10 to 35 parts by weight of coconut water, 2 parts by weight of peptone, 10 to 30 parts by weight of chop soup, and 10 to 30 parts by weight of sucrose, and is used for an artificial culturing method of cordyceps sinensis and providing a cordyceps sinensis product.
Description
Technical field
The present invention relates to a kind of method and medium of cultivating Cordyceps sinensis, relate in particular to a kind of method and medium of cultivating Cordyceps sinensis with manual type.
Background technology
Cordyceps sinensis is that a kind of formal name used at school is the fungi of Cordyceps sinensis, has another name called Chinese Chinese caterpillar fungus, but its infected insect, and it is dead to make it to ossify, and then grows fruit body, constitutes the Cordyceps sinensis complex with the worm corpse.
Cordyceps sinensis contains essential amino acid and protein, cordycepin, Chinese caterpillar fungus polysaccharide, mannitol, Cobastab
1, B
2, B
12, E and effective components such as K, rare mineral matter, essential fatty acid and steroid polysaccharides compound, thereby have drug effect and belong to traditional medicinal material with commercial value.
Natural Cordyceps sinensis complex collection is difficult for.The acquisition cost of obtaining natural Cordyceps sinensis complex from natural environment is high.Also and then in insect inoculate Cordyceps sinensis by breeding insects, low with the mode success rate of the Cordyceps sinensis complex of gathering, be difficult to effectively commercially produce.
The Cordyceps sinensis breeding method haves much room for improvement from the above, and market is to having required with the Cordyceps sinensis product.
Summary of the invention
Because the existing problem of adopting that natural Cordyceps sinensis complex collection is difficult for and producing difficulty, and, the object of the present invention is to provide a kind of medium, Cordyceps sinensis artificial culturing method and Cordyceps sinensis product that is used to cultivate Cordyceps sinensis for to satisfy the demand in market.
For achieving the above object, the invention provides a kind of medium that is used to cultivate Cordyceps sinensis, include: content is the water that divides of 1000 weight portions, content between the starch material of 500 to 1500 weight portions, content between the Sucus Cocois of 10 to 35 weight portions, peptone that content is 2 weight portions, the content big bone soup between 10 to 30 weight portions, and described big bone soup is that the water with 20 to 60 weight portions adds that the animal bone of 10 to 30 weight portions continues to be heated to the liquid of described aqueous evaporable to remaining 10 to 30 weight portions; And content is between the sucrose of 10 to 30 weight portions.
Described starch material is a grain powder; Described grain powder will be selected from group's cereal abrasive dust of being made up of rice, millet, jowar and wheat and form.
Preferably, the medium that is used to cultivate Cordyceps sinensis includes:
Content is the water of 1000 weight portions;
Content is the starch material of 1500 weight portions;
Content is the Sucus Cocois of 20 weight portions;
Content is the peptone of 2 weight portions;
Content is the big bone soup of 20 weight portions, and described big bone soup is that content is added that between the water of 20 to 60 weight portions content continues to be heated to described aqueous evaporable to the liquid of remaining content between 10 to 30 weight portions between the animal bone of 10 to 30 weight portions; And
Content is the sucrose of 20 weight portions.
This medium that is used to cultivate Cordyceps sinensis can be used for implementing the Cordyceps sinensis artificial culturing method.
The present invention is also relevant for the Cordyceps sinensis artificial culturing method.Cordyceps sinensis artificial culturing method of the present invention includes: form a medium, with described medium reduce to room temperature, described medium is inserted a container, heating described medium to 100 ℃ and continue 2 hours, with described medium be cooled to 10.0-12.0 ℃ with obtain a treated medium, in described treated culture medium inoculated Cordyceps sinensis to form a culture mix, the Cordyceps sinensis one-tenth of the growth of the Cordyceps sinensis in described mixed culture expansion, the expansion of will grow grown fruit body to form a Cordyceps sinensis product.
Cordyceps sinensis artificial culturing method of the present invention, it includes:
Content boils to form a medium between the sucrose mixing of 10-30 weight portion between big bone soup, the content of 10-30 weight portion between the Sucus Cocois of 10-35 weight portion, peptone, the content that content is 2 weight portions between starch material, the content of 500-1500 weight portion for water, the content with 1000 weight portions, and described big bone soup is that content is added that between the water of 20 to 60 weight portions content continues to be heated to described aqueous evaporable to the liquid of remaining content between 10 to 30 weight portions between the animal bone of 10 to 30 weight portions;
Described medium is reduced to room temperature;
Described medium is inserted a container, and this container has accommodation space, and described medium accounts for half of this accommodation space;
Heat described medium to 100 ℃ and continue 2 hours;
In desinfection chamber described medium is cooled to 10.0-12.0 ℃, to obtain a treated medium;
In described treated culture medium inoculated Cordyceps sinensis, to form a culture mix;
Keep described culture mix in 10.0-12.0 ℃ and a lasting growth time, so that the growth of the Cordyceps sinensis in this mixed culture expansion; And
Keep described culture mix in 18.0-20.0 ℃,, form a Cordyceps sinensis product so that the Cordyceps sinensis of growth expansion becomes to grow fruit body.
Wherein, the method for described inoculation Cordyceps sinensis is that fragment with Cordyceps sinensis places described medium.
Described growth time is three months.
Described Cordyceps sinensis product includes Cordyceps sinensis and the described treated medium that described one-tenth grows fruit body.
In addition, Cordyceps sinensis artificial culturing method of the present invention also can include:
Content is the water with 1000 weight portions, content is between the starch material of 500-1500 weight portion, content is between the Sucus Cocois of 10-35 weight portion, content is the peptone of 2 weight portions, content is between the big bone soup of 10-30 weight portion, content is inserted one between the sucrose of 10-30 weight portion and is had the container of accommodation space and the part of this accommodation space that accounts for approximately, and then also described big bone soup was that content is added that between the water of 20 to 60 weight portions content continues to be heated to described aqueous evaporable to the liquid of remaining content between 10 to 30 weight portions between the animal bone of 10 to 30 weight portions to form a medium in lasting 2 hours to be heated to 100 ℃;
In desinfection chamber described medium is cooled to 10.0-12.0 ℃;
In institute's culture medium inoculated Cordyceps sinensis, to form a culture mix;
Keep described culture mix in 10.0-12.0 ℃ and lasting three months;
Keep described culture mix in 18.0-20.0 ℃,, form a Cordyceps sinensis product so that the Cordyceps sinensis of long expansion becomes to grow fruit body.
The present invention is also relevant for the Cordyceps sinensis product that forms with preceding method.
Cordyceps sinensis product of the present invention, it is further handled through the processing of super-dry, abrasive dust and pill.
Can utilize the medium and the described method that are fit to commercially produce to produce Cordyceps sinensis in a large number by the present invention, and then provide the Cordyceps sinensis product to meet the need of market, reach purpose of the present invention.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
(1) is used to cultivate the medium of Cordyceps sinensis
The medium that is used to cultivate Cordyceps sinensis provided by the present invention includes:
Content is the water of 1000 weight portions;
Content is between the starch material of 500 to 1500 weight portions;
Content is between the Sucus Cocois of 10 to 35 weight portions;
Content is the peptone of 2 weight portions;
Content is between the big bone soup of 10 to 30 weight portions; And
Content is between the sucrose of 10 to 30 weight portions.
Described big bone soup is that the water with 20 to 60 weight portions adds that the animal bone of 10 to 30 weight portions continues to be heated to the liquid of described aqueous evaporable to remaining 10 to 30 weight portions.
Embodiment 1
Medium at embodiments of the invention 1 includes:
Content is the water of 1000 weight portions;
Content is the starch material of 1500 weight portions;
Content is the Sucus Cocois of 20 weight portions;
Content is the peptone of 2 weight portions;
Content is the big bone soup of 20 weight portions; And
Content is the sucrose of 20 weight portions.
The concrete deal of this each composition of medium can be:
Content is 1 liter water;
Content is 1.5 kilograms starch material;
Content is 20 public pinches Sucus Cocois;
Content is 2 g peptone;
Content is 20 public pinches big bone soup; And
Content is 20 g sucrose.
In embodiment 1, adopt grain powder as described starch material.This grain powder is that the cereal abrasive dust is formed, and the cereal that can adopt includes but is not limited to rice, millet, jowar and wheat.
(2) Cordyceps sinensis artificial culturing method
For forming a kind of Cordyceps sinensis product, Cordyceps sinensis artificial culturing method provided by the present invention includes:
Content boils to form a medium between the sucrose mixing of 10-30 weight portion between big bone soup, the content of 10-30 weight portion between the Sucus Cocois of 10-35 weight portion, peptone, the content that content is 2 weight portions between starch material, the content of 500-1500 weight portion for water, the content with 1000 weight portions;
Described medium is reduced to room temperature;
Described medium is inserted a container, and this container has accommodation space, and described medium accounts for half of this accommodation space;
Heat described medium to 100 ℃ and continue 2 hours;
In desinfection chamber described medium is cooled to 10.0-12.0 ℃, to obtain a treated medium;
In described treated culture medium inoculated Cordyceps sinensis, to form a culture mix;
Keep described culture mix in 10.0-12.0 ℃ and a lasting growth time, so that the growth of the Cordyceps sinensis in described mixed culture expansion; And
Keep described culture mix in 18.0-20.0 ℃,, form a Cordyceps sinensis product so that the Cordyceps sinensis of growth expansion becomes to grow fruit body.
Embodiment 2
Embodiments of the invention 2 adopt the medium of previous embodiment 1 to implement described Cordyceps sinensis artificial culturing method.
It is that the big bone soup and the content of 20 weight portions is the compositions such as sucrose of 20 weight portions that embodiments of the invention 2 boil water that content is 1000 weight portions, starch material that content is 1500 weight portions, Sucus Cocois that content is 20 weight portions, peptone, content that content is 2 weight portions earlier, forming the medium of described embodiment 1, and then implement subsequent step to form a Cordyceps sinensis product.
Described medium is reduced to room temperature earlier, is placed into a container again.Preferably, this container is a vial.This container has accommodation space, when described medium of reducing to room temperature is inserted this container, need not this container is filled up fully.Account for half of this accommodation space at medium described in the embodiment 2.
Then the described medium that is placed into container is carried out high temperature sterilization.High temperature sterilization means commonly used are that described container is placed into the cultivation of container based on being heated to 100 ℃ and lasting 2 hours in the pressure cooker, with the sterilization effects that reaches a high temperature together with described.When high temperature sterilization, usually this container is covered the medium of being packed in its accommodation space to protect in the breathable mode.
For avoiding living contaminants, through the described medium of high temperature sterilization can be placed in preserve in the cryogenic sterile environment standby.The cryogenic sterile equipment that is adopted at embodiment 2 is the desinfection chamber with air-conditioning.By described medium through high temperature sterilization is stored in desinfection chamber, except avoiding living contaminants, also can reach cooling-down effect by the air-conditioning of desinfection chamber.
Before inoculation Cordyceps sinensis, earlier described medium is cooled to 10.0-12.0 ℃, to obtain a treated medium.Then, in described treated culture medium inoculated Cordyceps sinensis, to form a culture mix.The method of described inoculation Cordyceps sinensis is that the fragment with Cordyceps sinensis places described medium to form culture mix.
After through inoculation, keep described culture mix in 10.0-12.0 ℃ and a lasting growth time.In embodiment 2, this growth time is three months, allows Cordyceps sinensis growth expansion in this mixed culture.Under desirable situation, through behind the growth time, the Cordyceps sinensis in this mixed culture can be grown and is expanded to the degree that covers this mixed culture integral body.
After through three months, keep described culture mix in 18.0-20.0 ℃, so that the Cordyceps sinensis of growth expansion becomes to grow fruit body, form a Cordyceps sinensis product.Though can remove remaining treated medium, allow this Cordyceps sinensis product only include the Cordyceps sinensis that described one-tenth grows fruit body, but in embodiment 2, do not remove remaining medium separately, therefore described Cordyceps sinensis product includes Cordyceps sinensis and the described treated medium that described one-tenth grows fruit body.
In addition, can handle for the processing that described Cordyceps sinensis product further carry out drying, abrasive dust and pill, to allow described Cordyceps sinensis product form wieldy formulation, also can carry out drying, abrasive dust is made into capsule.
Embodiment 3
With the described Cordyceps sinensis product that includes the Cordyceps sinensis that becomes to grow fruit body and described treated medium, after carrying out drying, abrasive dust, being chemically examined as can be known again, this Cordyceps sinensis product includes the vitamin C of 1.14ppm, the vitamin E of 10.63ppm, the vitamin A of 1.37ppm, the vitamin D of 0.15ppm, the Cobastab of 11.07ppm
1, 4.01ppm Cobastab
2, 132.41ppm Cobastab
6, 23.7ppm adenosine (adenosine), 1.47% cordycepic acid and 20.78% the water dissolvable polysaccharides of copper, 136.48ppm of calcium, 2.87ppm of iron, 901ppm of magnesium, 72.2ppm of zinc, 1171ppm.
In addition, described Cordyceps sinensis product has the activity of the superoxide dismutase of 5.96IU/g.
Thereby the main pharmacodynamics composition that has Cordyceps sinensis as can be known by previous embodiment 2 formed Cordyceps sinensis products really.
(3) another Cordyceps sinensis artificial culturing method
For forming described Cordyceps sinensis product, another Cordyceps sinensis artificial culturing method provided by the present invention includes:
Content has the container of accommodation space and the part of this accommodation space that accounts for approximately for the water of 1000 weight portions, content are inserted one between big bone soup, the content of 10-30 weight portion between the sucrose of 10-30 weight portion between the Sucus Cocois of 10-35 weight portion, peptone, content that content is 2 weight portions between the starch material of 500-1500 weight portion, content, and then is heated to 100 ℃ and continue 2 hours to form a medium;
In desinfection chamber described medium is cooled to 10.0-12.0 ℃;
In described culture medium inoculated Cordyceps sinensis, to form a culture mix;
Keep described culture mix in 10.0-12.0 ℃ and lasting three months;
Keep described culture mix in 18.0-20.0 ℃,, form a Cordyceps sinensis product so that the Cordyceps sinensis of growth expansion becomes to grow fruit body.
This another Cordyceps sinensis artificial culturing method and aforementioned Cordyceps sinensis artificial culturing method are roughly the same, its institute is different to be in when using this another Cordyceps sinensis artificial culturing method, the container of earlier each composition being packed into to scale is combined into a step will boil with high temperature sterilization.
Embodiment 4
Embodiments of the invention 4 adopt the medium of previous embodiment 1 to implement described another Cordyceps sinensis artificial culturing method.
The Sucus Cocois that embodiments of the invention 2 are earlier the water of 1000 weight portions, starch material that content is 1500 weight portions with content, content is 20 weight portions, peptone, the content that content is 2 weight portions are that the big bone soup of 20 weight portions and compositions such as sucrose that content is 20 weight portions are inserted one and had the container of accommodation space and the part of this accommodation space that accounts for approximately, and then be heated to 100 ℃ and lasting 2 hours medium with formation previous embodiment 1, implement the subsequent step of described another Cordyceps sinensis artificial culturing method to form a Cordyceps sinensis product with this medium again.
Concrete manufacturing of the present invention from the above and implementation method, it really can solve the problem that the Cordyceps sinensis collection is difficult for and produces difficulty, and satisfies the demand in market, thereby can reach purpose of the present invention.
Claims (10)
1. a medium that is used to cultivate Cordyceps sinensis is characterized in that, described medium includes:
Content is the water of 1000 weight portions;
Content is between the starch material of 500 to 1500 weight portions;
Content is between the Sucus Cocois of 10 to 35 weight portions;
Content is the peptone of 2 weight portions;
Content is between the big bone soup of 10 to 30 weight portions, and described big bone soup is that the water with 20 to 60 weight portions adds that the animal bone of 10 to 30 weight portions continues to be heated to the liquid of described aqueous evaporable to remaining 10 to 30 weight portions; And
Content is between the sucrose of 10 to 30 weight portions.
2. medium as claimed in claim 1 is characterized in that described starch material is a grain powder; Described grain powder will be selected from group's cereal abrasive dust of being made up of rice, millet, jowar and wheat and form.
3. medium as claimed in claim 1 is characterized in that it includes:
Content is the water of 1000 weight portions;
Content is the starch material of 1500 weight portions;
Content is the Sucus Cocois of 20 weight portions;
Content is the peptone of 2 weight portions;
Content is the big bone soup of 20 weight portions, and described big bone soup is that content is added that between the water of 20 to 60 weight portions content continues to be heated to described aqueous evaporable to the liquid of remaining content between 10 to 30 weight portions between the animal bone of 10 to 30 weight portions; And
Content is the sucrose of 20 weight portions.
4. a Cordyceps sinensis artificial culturing method is characterized in that, described artificial culture method includes:
Content boils to form a medium between the sucrose mixing of 10-30 weight portion between big bone soup, the content of 10-30 weight portion between the Sucus Cocois of 10-35 weight portion, peptone, the content that content is 2 weight portions between starch material, the content of 500-1500 weight portion for water, the content with 1000 weight portions, and described big bone soup is that content is added that between the water of 20 to 60 weight portions content continues to be heated to described aqueous evaporable to the liquid of remaining content between 10 to 30 weight portions between the animal bone of 10 to 30 weight portions;
Described medium is reduced to room temperature;
Described medium is inserted a container, and this container has accommodation space, and described medium accounts for half of this accommodation space;
Heat described medium to 100 ℃ and continue 2 hours;
In desinfection chamber described medium is cooled to 10.0-12.0 ℃, to obtain a treated medium;
In described treated culture medium inoculated Cordyceps sinensis, to form a culture mix;
Keep described culture mix in 10.0-12.0 ℃ and a lasting growth time, so that the growth of the Cordyceps sinensis in this mixed culture expansion; And
Keep described culture mix in 18.0-20.0 ℃,, form a Cordyceps sinensis product so that the Cordyceps sinensis of growth expansion becomes to grow fruit body.
5. Cordyceps sinensis artificial culturing method as claimed in claim 4 is characterized in that, the method for described inoculation Cordyceps sinensis is that the fragment with Cordyceps sinensis places described medium.
6. Cordyceps sinensis artificial culturing method as claimed in claim 5 is characterized in that described growth time is three months.
7. Cordyceps sinensis artificial culturing method as claimed in claim 6 is characterized in that, described Cordyceps sinensis product includes Cordyceps sinensis and the described treated medium that described one-tenth grows fruit body.
8. a Cordyceps sinensis artificial culturing method is characterized in that, described artificial culture method includes:
Content is the water with 1000 weight portions, content is between the starch material of 500-1500 weight portion, content is between the Sucus Cocois of 10-35 weight portion, content is the peptone of 2 weight portions, content is between the big bone soup of 10-30 weight portion, content is inserted one between the sucrose of 10-30 weight portion and is had the container of accommodation space and the part of this accommodation space that accounts for approximately, and then also described big bone soup was that content is added that between the water of 20 to 60 weight portions content continues to be heated to described aqueous evaporable to the liquid of remaining content between 10 to 30 weight portions between the animal bone of 10 to 30 weight portions to form a medium in lasting 2 hours to be heated to 100 ℃;
In desinfection chamber described medium is cooled to 10.0-12.0 ℃;
In described culture medium inoculated Cordyceps sinensis, to form a culture mix;
Keep described culture mix in 10.0-12.0 ℃ and lasting three months;
Keep described culture mix in 18.0-20.0 ℃,, form a Cordyceps sinensis product so that the Cordyceps sinensis of long expansion becomes to grow fruit body.
9. a Cordyceps sinensis product is characterized in that, described Cordyceps sinensis product is to be formed as each described Cordyceps sinensis artificial culturing method in the claim 1 to 5.
10. as claim 9 a described Cordyceps sinensis product, it is characterized in that it is further handled through the processing of super-dry, abrasive dust and pill.
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| CN201010203872XA CN102283009B (en) | 2010-06-17 | 2010-06-17 | Culture medium used for culturing cordyceps sinensis, artificial culturing method of cordyceps sinensis and cordyceps sinensis product |
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| CN201010203872XA CN102283009B (en) | 2010-06-17 | 2010-06-17 | Culture medium used for culturing cordyceps sinensis, artificial culturing method of cordyceps sinensis and cordyceps sinensis product |
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| CN103250558A (en) * | 2013-04-16 | 2013-08-21 | 东至林野生态种植中心 | Cultivation method of sea-buckthorn bagged health monkey head mushroom |
| CN112931045A (en) * | 2021-02-03 | 2021-06-11 | 王鹏 | Culture medium suitable for culturing various fungal mycelia and preparation method thereof |
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| CN101418263A (en) * | 2007-10-23 | 2009-04-29 | 崔学恒 | Bacterium culture medium of northern cordyceps wine |
| CN101663964A (en) * | 2009-10-09 | 2010-03-10 | 浙江天使生物工程有限公司 | Cordyceps militaris fruit body culture medium and preparation method thereof |
| CN101690463A (en) * | 2009-08-21 | 2010-04-07 | 吉林大学 | Mutagenic strain of cordyceps militaris and breeding method |
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| CN101418263A (en) * | 2007-10-23 | 2009-04-29 | 崔学恒 | Bacterium culture medium of northern cordyceps wine |
| CN101690463A (en) * | 2009-08-21 | 2010-04-07 | 吉林大学 | Mutagenic strain of cordyceps militaris and breeding method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103250558A (en) * | 2013-04-16 | 2013-08-21 | 东至林野生态种植中心 | Cultivation method of sea-buckthorn bagged health monkey head mushroom |
| CN103250558B (en) * | 2013-04-16 | 2014-10-29 | 东至林野生态种植中心 | Cultivation method of sea-buckthorn bagged health monkey head mushroom |
| CN112931045A (en) * | 2021-02-03 | 2021-06-11 | 王鹏 | Culture medium suitable for culturing various fungal mycelia and preparation method thereof |
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| CN102283009B (en) | 2012-09-19 |
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