CN106747848A - South Korea's Phellinus bacterium plastic bag cultivation method - Google Patents
South Korea's Phellinus bacterium plastic bag cultivation method Download PDFInfo
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- CN106747848A CN106747848A CN201710205285.6A CN201710205285A CN106747848A CN 106747848 A CN106747848 A CN 106747848A CN 201710205285 A CN201710205285 A CN 201710205285A CN 106747848 A CN106747848 A CN 106747848A
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- fructification
- bag
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- phellinus
- bacterium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of cultivation technique of fungi plant, and in particular to be a kind of South Korea's Phellinus bacterium plastic bag cultivation method, by culture medium by recipe ratio put mixer add water stir;Packed using polypropylene edible mushroom bag;Use gerotor type steam sterilizing;It is cooled in 45 DEG C of 60 DEG C of immigration desinfection chambers and stacks, be cooled to 25 DEG C 30 DEG C, can be aseptically inoculated with;Culturing room is moved into after inoculation is good, the time covered with mycelia for 30 40 days;The bacterium bag that mycelia will be covered with moves into mushroom producing room into fructification breeding phase, has discharged bacterium bag, pkt. flap is opened, until going out Huang;Harvesting after 120 180 days fructification maturations, Softening gently adopts fructification on the other hand to hold bacterium bag on the other hand, then dries, packs;The present invention maintains the effective ingredient of South Korea's Phellinus bacterial strain fructification and mycelium pharmacology to the full extent, through testing above method, various contents of the fructification cultivated with its various component content of mycelium equivalent to wild Phellinus.
Description
Technical field
The present invention relates to a kind of cultivation technique of fungi plant, and in particular to be a kind of South Korea's Phellinus bacterium plastic bag cultivation
Method.
Background technology
South Korea's Phellinus is the new rare medicinal herbs for emerging in current medicinal fungi kind, is also internationally recognized biological anti-
Efficiency highest medicinal fungi in cancer, anti-knurl field, wild Phellinus is formed in South Korea's nature can use fructification rareness, it is difficult to
Meet the market demand, add many people's exploitations, wild Phellinus is close to commit genocide.
Internation combination carries out widely studied to the medicinal efficacy of South Korea Phellinus bacterium, it is found that South Korea Phellinus has antitumor, anti-
Cancer, anti-oxidant, enhance immunity, adjust for treat metrorrhagia, prolapse of the anus, it is stagnant under, amenorrhoea, leukorrhea, hard abdominal mass, the cancer of the uterus, son
Palace knurl, pruritus vulvae, negative and positive fever and chills s.m.p disease, enter from differentiation, and stasis of blood hemostasis follows, through and row bleeding stops, in rushing down blood, splenasthenic diarrhea, food
Malicious, allergic, hemorrhoid, stomachache, hypoglycemic etc., to various cancers all putting prevention first in advance.To cancer, knurl patient before the second phase
With play the role of very big after late surgery, Phellinus liquid can be voluntarily dead with bloody path circulation-induced cancer cell, suppresses oncocyte and turns
Move, mitigate the side effect of chemotherapy and radiation, prevent ulcer, recurrence of the polyp to benign tumour canceration cancer.
South Korea Phellinus bacterium also has liver protection effect, can anti-hepatic fibrosis, promote liver cell regeneration, prevent and treat chronic hepatitis, liver hard
Change, liver ascites, reduce and adjustment blood sugar solubility, the generation of prevention of cerebrovascular diseases.
Phellinus strain of the invention is introduced projects by national Shanghai academy of agricultural sciences and obtained, and fructification is carried out tissue point through me
From, covered with to mycelia, by purification master clock, part switching original seed covers with preservation to mycelia.
The content of the invention
It is an object of the invention to overcome above-mentioned deficiency, there is provided a kind of South Korea's Phellinus bacterium plastic bag cultivation method.
The technical solution adopted in the present invention is such:A kind of South Korea's Phellinus bacterium plastic bag cultivation method, including it is as follows
Step:(1)Culture medium:Culture medium is put into mixer by recipe ratio adds water to stir, and mixing time is:15-20 minutes;
(2)Pack:Packed using polypropylene edible mushroom bag, bagging ring cowling;
(3)Sterilization:Gerotor type steam sterilizing is used, temperature is 98 DEG C -102 DEG C, time 10-14 hours;
(4)Inoculation:It is cooled in 45 DEG C of -60 DEG C of immigration desinfection chambers and stacks, be cooled to 25 DEG C -30 DEG C, aseptically can
Inoculation;
(5)Cultural hypha:Culturing room is moved into after inoculation is good, at 20 DEG C -30 DEG C, the time covered with mycelia to temperature control for 30-40 days;
(6)Fructification culture:The bacterium bag that mycelia will be covered with moves into mushroom producing room into fructification breeding phase, has discharged bacterium bag, beats
Pkt. flap is opened, until going out Huang, temperature is 20-30 DEG C;
(7)Harvesting:Plucked after 120-180 days fructification maturations, Softening gently adopts fructification on the other hand to hold bacterium bag on the other hand,
Then dry, pack;
(8)Final-period management:After first fructification is plucked, allow mycelia to recover 5-10 days, carry out second batch growth;
(9)Mycelium is processed:Compost mycelium is taken off bag, drying and packaging by harvesting after terminating.
Preferably, the step(1)In culture medium, be obtained by the raw material of following weight portion:Mulberry tree considers 35%-45% to be worth doing, miscellaneous
Wood chip 20%-30%, cotton seed hulls 10%-17%, wheat skin 11%-18%, corn flour 6%-12%, dregs of beans 3%-8%, land plaster 1%-3%, brown sugar
1%-2%, crosses calcium 1%-1.5%, carbonic acid powder 1%-3%.
Preferably, the step(1)The stirring water content of culture medium is 60-68%.
Preferably, the step(5)The air humidity of cultural hypha is 45-60%.
Preferably, the step(6)Fructification culture in humidity be 80-90%.
By using preceding solution, the beneficial effects of the invention are as follows::1st, life of the present invention according to South Korea Phellinus bacterium
Characteristic long and effective medical value deep layer research, to Phellinus bacteria cultivation and cultivation back to nature.Korea Spro is maintained to the full extent
The effective ingredient of state's Phellinus bacterial strain fructification and mycelium pharmacology.
2nd, purity is high, free from admixture, pollution-free
3rd, color and luster is beautiful, sense organ is good, yield is high
4th, through testing above method, its various component content of the fructification cultivated and mycelium are various equivalent to wild Phellinus
Content.
Figure of description
Fig. 1 is the fructification for growing of the invention.
Specific embodiment
The present invention is specifically described with embodiment below, the scope of the present invention should not be limited by the examples.
Embodiment 1
A kind of South Korea's Phellinus bacterium plastic bag cultivation method, comprises the following steps:(1)Culture medium:Consider mulberry tree to be worth doing 40%, weed tree sawdust
20%, cotton seed hulls 15%, wheat skin 12%, corn flour 6%, dregs of beans 3%, land plaster 1%, brown sugar 1% crosses calcium 1%, and carbonic acid powder 1% presses recipe ratio
Put mixer and add water stirring, mixing time 15 minutes, stirring water content is 60%;
(2)Pack:Packed using polypropylene edible mushroom bag, bagging ring cowling;
(3)Sterilization:Gerotor type steam sterilizing is used, temperature is 98 DEG C, 11 hours time;
(4)Inoculation:It is cooled in 50 DEG C of immigration desinfection chambers and stacks, be cooled to 25 DEG C, can be aseptically inoculated with;
(5)Cultural hypha:Culturing room is moved into after inoculation is good, at 20 DEG C, air humidity is 45% to temperature control, the time is 35 days long
Full mycelia;
(6)Fructification culture:The bacterium bag that mycelia will be covered with moves into mushroom producing room into fructification breeding phase, has discharged bacterium bag, beats
Pkt. flap is opened, until going out Huang, temperature is 20 DEG C, and humidity is 80%;
(7)Harvesting:Plucked after 150 days fructification maturations, Softening gently adopts fructification, then on the other hand to hold bacterium bag on the other hand
Drying, packaging;
(8)Final-period management:After first fructification is plucked, allow mycelia to recover 6 days, carry out second batch growth;
(9)Mycelium is processed:Compost mycelium is taken off bag, drying and packaging by harvesting after terminating.
Embodiment 2
A kind of South Korea's Phellinus bacterium plastic bag cultivation method, comprises the following steps:(1)Culture medium:Consider mulberry tree to be worth doing 45%, weed tree sawdust
20%, cotton seed hulls 11%, wheat skin 12%, corn flour 6%, dregs of beans 3%, land plaster 1%, brown sugar 1% crosses calcium 1%, and carbonic acid powder 1% presses recipe ratio
Put mixer and add water stirring, mixing time is:20 minutes, stirring water content was 68%;
(2)Pack:Packed using polypropylene edible mushroom bag, bagging ring cowling;
(3)Sterilization:Gerotor type steam sterilizing is used, temperature is 102 DEG C, 14 hours time;
(4)Inoculation:It is cooled in 60 DEG C of immigration desinfection chambers and stacks, be cooled to 30 DEG C, can be aseptically inoculated with;
(5)Cultural hypha:Culturing room is moved into after inoculation is good, at 30 DEG C, air humidity is 60% to temperature control, the time is 40 days long
Full mycelia;
(6)Fructification culture:The bacterium bag that mycelia will be covered with moves into mushroom producing room into fructification breeding phase, has discharged bacterium bag, beats
Pkt. flap is opened, until going out Huang, temperature is 30 DEG C, and humidity is 90%;
(7)Harvesting:Plucked after 180 days fructification maturations, Softening gently adopts fructification, then on the other hand to hold bacterium bag on the other hand
Drying, packaging;
(8)Final-period management:After first fructification is plucked, allow mycelia to recover 10 days, carry out second batch growth;
(9)Mycelium is processed:Compost mycelium is taken off bag, drying and packaging by harvesting after terminating.
Embodiment 3
A kind of South Korea's Phellinus bacterium plastic bag cultivation method, comprises the following steps:(1)Culture medium:Consider mulberry tree to be worth doing 40%, weed tree sawdust
20%, cotton seed hulls 10%, wheat skin 13%, corn flour 7%, dregs of beans 5%, land plaster 2%, brown sugar 1% crosses calcium 1%, and carbonic acid powder 1% presses recipe ratio
Put mixer and add water stirring, mixing time is:18 minutes, stirring water content was 65%;
(2)Pack:Packed using polypropylene edible mushroom bag, bagging ring cowling;
(3)Sterilization:Gerotor type steam sterilizing is used, temperature is 100 DEG C, 12 hours time;
(4)Inoculation:It is cooled in 50 DEG C of immigration desinfection chambers and stacks, be cooled to 28 DEG C, can be aseptically inoculated with;
(5)Cultural hypha:Culturing room is moved into after inoculation is good, at 28 DEG C, air humidity is 52% to temperature control, the time is 35 days long
Full mycelia;
(6)Fructification culture:The bacterium bag that mycelia will be covered with moves into mushroom producing room into fructification breeding phase, has discharged bacterium bag, beats
Pkt. flap is opened, until going out Huang, temperature is 26 DEG C, and humidity is 86%;
(7)Harvesting:Plucked after 150 days fructification maturations, Softening gently adopts fructification, then on the other hand to hold bacterium bag on the other hand
Drying, packaging;
(8)Final-period management:After first fructification is plucked, allow mycelia to recover 8 days, carry out second batch growth;
(9)Mycelium is processed:Compost mycelium is taken off bag, drying and packaging by harvesting after terminating.
Embodiment 4
A kind of South Korea's Phellinus bacterium plastic bag cultivation method, comprises the following steps:(1)Culture medium:Consider mulberry tree to be worth doing 35%, weed tree sawdust
25%, cotton seed hulls 17%, wheat skin 11%, corn flour 6%, dregs of beans 3%, land plaster 1%, brown sugar 1% crosses calcium 1%, and carbonic acid powder 1% presses recipe ratio
Put mixer and add water stirring, mixing time is:16 minutes, stirring water content was 62%;
(2)Pack:Packed using polypropylene edible mushroom bag, bagging ring cowling;
(3)Sterilization:Gerotor type steam sterilizing is used, temperature is 99 DEG C, 12 hours time;
(4)Inoculation:It is cooled in 58 DEG C of immigration desinfection chambers and stacks, be cooled to 26 DEG C, can be aseptically inoculated with;
(5)Cultural hypha:Culturing room is moved into after inoculation is good, at 25 DEG C, air humidity is 56% to temperature control, the time is 38 days long
Full mycelia;
(6)Fructification culture:The bacterium bag that mycelia will be covered with moves into mushroom producing room into fructification breeding phase, has discharged bacterium bag, beats
Pkt. flap is opened, until going out Huang, temperature is 28 DEG C, and humidity is 88%;
(7)Harvesting:Plucked after 160 days fructification maturations, Softening gently adopts fructification, then on the other hand to hold bacterium bag on the other hand
Drying, packaging;
(8)Final-period management:After first fructification is plucked, allow mycelia to recover 7 days, carry out second batch growth;
(9)Mycelium is processed:Compost mycelium is taken off bag, drying and packaging by harvesting after terminating.
The above, specific embodiment only of the invention, but protection scope of the present invention is not limited thereto, and it is any
Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all contain
Cover within protection scope of the present invention.Therefore, protection scope of the present invention described should be defined by scope of the claims.
Claims (5)
1. a kind of South Korea's Phellinus bacterium plastic bag cultivation method, it is characterised in that:Comprise the following steps:(1)Culture medium:By culture medium
Putting mixer by recipe ratio adds water to stir, and mixing time is:15-20 minutes;
(2)Pack:Packed using polypropylene edible mushroom bag, bagging ring cowling;
(3)Sterilization:Gerotor type steam sterilizing is used, temperature is 98 DEG C -102 DEG C, time 10-14 hours;
(4)Inoculation:It is cooled in 45 DEG C of -60 DEG C of immigration desinfection chambers and stacks, be cooled to 25 DEG C -30 DEG C, aseptically can
Inoculation;
(5)Cultural hypha:Culturing room is moved into after inoculation is good, at 20 DEG C -30 DEG C, the time covered with mycelia to temperature control for 30-40 days;
(6)Fructification culture:The bacterium bag that mycelia will be covered with moves into mushroom producing room into fructification breeding phase, has discharged bacterium bag, beats
Pkt. flap is opened, until going out Huang, temperature is 20-30 DEG C;
(7)Harvesting:Plucked after 120-180 days fructification maturations, Softening gently adopts fructification on the other hand to hold bacterium bag on the other hand,
Then dry, pack;
(8)Final-period management:After first fructification is plucked, allow mycelia to recover 5-10 days, carry out second batch growth;
(9)Mycelium is processed:Compost mycelium is taken off bag, drying and packaging by harvesting after terminating.
2. a kind of South Korea's Phellinus bacterium plastic bag cultivation method according to claim 1, it is characterised in that:The step(1)
In culture medium, be obtained by the raw material of following weight portion:Mulberry tree considers 35%-45%, weed tree sawdust 20%-30%, cotton seed hulls 10%-17% to be worth doing,
Wheat skin 11%-18%, corn flour 6%-12%, dregs of beans 3%-8%, land plaster 1%-3%, brown sugar 1%-2%, cross calcium 1%-1.5%, carbonic acid powder
1%-3%。
3. a kind of South Korea's Phellinus bacterium plastic bag cultivation method according to claim 1, it is characterised in that:The step(1)
The stirring water content of culture medium is 60-68%.
4. a kind of South Korea's Phellinus bacterium plastic bag cultivation method according to claim 1, it is characterised in that:The step(5)
The air humidity of cultural hypha is 45-60%.
5. a kind of South Korea's Phellinus bacterium plastic bag cultivation method according to claim 1, it is characterised in that:The step(6)
Fructification culture in humidity be 80-90%.
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108174741A (en) * | 2017-11-29 | 2018-06-19 | 杭州千岛湖兴宝菇业专业合作社 | A kind of gold mushroom cultivation method |
CN109496692A (en) * | 2018-11-28 | 2019-03-22 | 上海市农业科学院 | A kind of Phellinus culture medium and its application |
CN109832091A (en) * | 2019-01-20 | 2019-06-04 | 香格里拉市腊玛农产品开发有限公司 | A kind of cultural method of the golden ear of half bag of mulberry wood chips plantation |
CN109832093A (en) * | 2019-03-14 | 2019-06-04 | 湖北省农业科学院经济作物研究所 | A method of Phellinus is cultivated using ramulus mori |
CN112608852A (en) * | 2021-01-06 | 2021-04-06 | 袁隆平农业高科技股份有限公司 | Inoculation and propagation method of fusarium verticillioides |
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CN102786333A (en) * | 2012-06-21 | 2012-11-21 | 杭州清正生物科技有限公司 | Phellinus igniarius bag cultivation medium and method for cultivating phellinus igniarius sporophore by same |
CN104381009A (en) * | 2014-09-18 | 2015-03-04 | 杭州千岛湖桑之宝农业开发有限公司 | Artificial bag cultivation process for Phellinus igniarius |
CN105993593A (en) * | 2016-05-23 | 2016-10-12 | 浙江省农业科学院 | High-efficiency industrial bag material cultivation process of inonotus linteus with antitumor activity |
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CN102786333A (en) * | 2012-06-21 | 2012-11-21 | 杭州清正生物科技有限公司 | Phellinus igniarius bag cultivation medium and method for cultivating phellinus igniarius sporophore by same |
CN104381009A (en) * | 2014-09-18 | 2015-03-04 | 杭州千岛湖桑之宝农业开发有限公司 | Artificial bag cultivation process for Phellinus igniarius |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108174741A (en) * | 2017-11-29 | 2018-06-19 | 杭州千岛湖兴宝菇业专业合作社 | A kind of gold mushroom cultivation method |
CN109496692A (en) * | 2018-11-28 | 2019-03-22 | 上海市农业科学院 | A kind of Phellinus culture medium and its application |
CN109832091A (en) * | 2019-01-20 | 2019-06-04 | 香格里拉市腊玛农产品开发有限公司 | A kind of cultural method of the golden ear of half bag of mulberry wood chips plantation |
CN109832093A (en) * | 2019-03-14 | 2019-06-04 | 湖北省农业科学院经济作物研究所 | A method of Phellinus is cultivated using ramulus mori |
CN112608852A (en) * | 2021-01-06 | 2021-04-06 | 袁隆平农业高科技股份有限公司 | Inoculation and propagation method of fusarium verticillioides |
CN112608852B (en) * | 2021-01-06 | 2023-06-09 | 袁隆平农业高科技股份有限公司 | Fusarium verticillium inoculation and propagation method |
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