CN108174741A - A kind of gold mushroom cultivation method - Google Patents
A kind of gold mushroom cultivation method Download PDFInfo
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- CN108174741A CN108174741A CN201711232434.4A CN201711232434A CN108174741A CN 108174741 A CN108174741 A CN 108174741A CN 201711232434 A CN201711232434 A CN 201711232434A CN 108174741 A CN108174741 A CN 108174741A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The present invention relates to a kind of gold mushroom cultivation method, the method includes selection cultivation season with place, selection planting material, cultivation strain, inoculation cultivation with burying wood；It wherein cultivates the culture medium used in strain and includes cotton seed hulls, mulberry wood chips, weed tree sawdust, wheat bran, corn flour, lime and auxiliary material.The implementation of the present invention can provide high-quality green agricultural product for market, for peasant's increasing both production and income, it can be utilized again based on the large-scale production of this county's characteristic new medicinal resources and deep development, achievement in research may for oncotherapy, inflammation treatment, anti-bacterial vaccines and other etc. important diseases new medicine source selection is provided, serve healthy cause.Meanwhile the industry to match as one and mulberry tree garden resource, gold mushroom will become the distinctive new industry in Chunan County, development prospect is good.
Description
Technical field
The present invention relates to field of edible fungus culture, are related to the cultivation of gold mushroom more particularly to a kind of with short production cycle, cost
Low, safe and reliable gold mushroom cultivation method.
Background technology
Edible Fungi undergoes the development of nearly half a century, and gross annual output amount has leapt to the first in the world, at home agriculture total yield
Grain, cotton, oil, fruit, dish are only second in value and occupies the 6th, has been more than Tea Industry and sericulture, oneself becomes an important agricultural high-effiency kind
It plants.Edible mushroom is domestic, international market vegetables treasure salable.Since edible fungi nutrition enriches, crude protein, amino acid and dimension life
Cellulose content is high, and with healthcare function, by the welcome and favor of consumer.In recent years, with medical, edible bacterium and its health care
Functional study is goed deep into, and the consumption market of edible mushroom is made constantly to expand, become medical industry, food industry important source material.
Edible mushroom is macro fungi, edible and the mushroom with medical value, is consumer important in the ecosystem,
The organic waste that the mankind such as sawdust, the lignin of crop material, cellulose, feces of livestock and poultry and domestic animal can be difficult to be utilized in they
(or byproduct) decomposition, utilization, conversion are provided for the mankind with delicious flavour full of nutrition, health functional food, are decomposed through it
Bacteria residue after utilization can then be processed into feed or organic fertilizer, back in plantation, aquaculture, become the repeatable money utilized
Source.The development of Rare edible fungus industry can not only extend the industrial chain of featured agriculture, increase output, make agriculture overall efficiency not
It is disconnected to improve, and a variety of organic wastes are converted into the new resource such as feed or compound fertilizer, soilless culture material, reduce pair
The pollution of ecological environment is conducive to the protection of ecological environment.
Gold mushroom nutritional ingredient be per 100g dry products 1.89g containing asparatate, threonine 1.01g, serine 1.04g,
It is glutamic acid 3.83g, glycine 1.06g, alanine 1.32g, valine 1.67g, methionine 1.02g, isoleucine 0.84g, bright
Propylhomoserin 1.31g, tyrosine 0.64g, phenylalanine 0.83g, lysine 1.01g, histidine 0.49g, arginine 1.06g, dried meat ammonia
Sour 0.96g, total amino acid 20.13g.Its is full of nutrition, it has also become rare food materials in high-quality life.But because of gold mushroom
The wild place of production and yield are all very limited, and resource is increasingly poor, and market supply is insufficient.The annual only wild product in China is whole
It exports to foreign countries, therefore gold massee fruiting bodies artificial cultivation is the only way of gold mushroom industrialized development.
It is mainly Japan and South Korea that preferable country is cultivated to gold mushroom in the world at present.The two countries are now real
The artificial cultivation of existing gold mushroom, can produce in batches, but growth cycle is long substantially, international and demand of the domestic market to gold mushroom
Still it is very big.
Gold mushroom is the new varieties manually developed, wild rare numbers, and growth district is extremely limited, and acquisition is very tired
Difficulty, the requirement of its cultivation condition is harsh in addition, and slow-growing, product is very in short supply, is hardly visible this product on the market at present.
For wild gold mushroom resource it is increasingly poor the phenomenon that, people have begun explore artificial cultivation obtain gold massee fruiting bodies side
Method.External research starting is more early, particularly South Korea and Japan, the people that gold mushroom has just been succeeded in South Korea before 2008
Work is cultivated.In China, the history for also having more than 20 years is explored in the artificial cultivation research of gold mushroom in China, due to its growth conditions
Special, culturing raw material and cultivation technique are particular about very much, and the commercialization artificial cultivation of gold mushroom is just started to walk.
Invention content
It is an object of the invention to harsh in order to solve existing gold mushroom cultivation condition requirement, slow-growing, product is very
The defects of in short supply and a kind of gold mushroom cultivation method with short production cycle, at low cost, safe and reliable is provided.
To achieve these goals, the present invention uses following technical scheme：
A kind of gold mushroom cultivation method, the method includes selection cultivation season and place, selection planting material, cultivate strain,
Inoculation cultivation is with burying wood；Wherein cultivate the raw material that the culture medium used in strain includes following parts by weight：15-25 parts of cotton seed hulls,
20-35 parts of mulberry wood chips, 30-35 parts of weed tree sawdusts, 15-22 parts of wheat brans, 1-5 portions of corn flour, 1-3 parts of lime and 3-7 parts of auxiliary materials.
Preferably, the auxiliary material is active peptides calcium chelates of zinc, Rosmarinic acid and xanthyletin.
In the technical program, in the course of cultivation, white ant is one of natural enemy of gold mushroom, the rosemary in the present invention
The combination of acid and xanthyletin, forms synergistic effect, can prevent damage of the white ant to gold mushroom.
Preferably, the preparation method of active peptides calcium chelates of zinc is：
A) it ferments：Active peptides are prepared with actinomycete fermentation；Fermentation step is：Weigh 33 parts of analysis for soybean powder, 10 parts of starch, yeast extract
7 parts, 15 parts of sucrose, 1 part of sodium chloride, 4 parts of peptone, 1 part of dipotassium hydrogen phosphate, 1 part of epsom salt, 20 parts of glucose, carbonic acid
3 parts of calcium, 1200 parts of distilled water, adjust pH7.2-7.4, and 112-114 DEG C of sterilizing obtains fermentation culture in 10-15 minutes；With training of fermenting
5% inoculation actinomyces starter of nutrient solution quality, ferment 2-4 hours to obtain zymotic fluid under 28-30 DEG C of ventilation condition；
B) it purifies：By centrifuging purification active peptides are purified with ammonium sulfate precipitation；Purification step is：Zymotic fluid is placed in centrifuge
In, it is centrifuged under the conditions of 4000-4500r/min 10-15 minutes in 35-38 DEG C of temperature, takes supernatant；By supernatant at 4-6 DEG C
Lower refrigerated centrifuge, lower centrifugation 10-15 minutes, take supernatant under the conditions of rotating speed 7500-8000r/min；By finely ground ammonium sulfate
Saturation degree according to 55-60% is added in above-mentioned supernatant, is centrifuged after being preserved overnight at 4-6 DEG C with 9500-10000r/min
30-45 minutes, the distilled water that precipitation adds in 2-3 times is taken to stir to obtain active peptides solution；
C) it chelates：Prepare active peptides calcium chelates of zinc；Chelation step is：The active peptides solution after purification is taken, under stiring
Add in 3-4.5 times of calcium chloride water containing 10% mass fraction measured with 1.1-1.4 times measure containing 7% mass fraction
Solder(ing)acid adjusts pH as 7-7.2 using hydrochloric acid and sodium hydroxide solution, concussion synthesis 35 minutes at a temperature of 20-22 DEG C,
Obtain active peptides calcium chelates of zinc.
Preferably, bacteria condition is 23-25 DEG C of temperature, air humidity 60-65%.
Preferably, the water content of culture medium is 60%.
Preferably, Cultivation condition is 18-22 DEG C, air humidity 80-85%, light application time 6h/d.
Preferably, the mass ratio of the active peptides calcium chelates of zinc, Rosmarinic acid and xanthyletin is 3: 1: 1.
Beneficial effects of the present invention：The present invention is main raw material(s) using mulberry branch, crushes bag cultivating gold mushroom.Base
Place region in addition to tourist resources, kind aquaculture it is most flourishing be sericulture, there is abundant mulberry branch resource, it is real for the present invention
Apply and provide sufficient raw material, at the same bacterial manure can " back feeding " mulberry tree garden, turn waste into wealth, comprehensively utilize, realize benign cycle.
The implementation of the present invention can provide high-quality green agricultural product for market, be peasant's increasing both production and income, and can be based on this
The large-scale production of county's characteristic new medicinal resources and deep development utilize, and achievement in research may be oncotherapy, inflammation treatment, antibacterial
Vaccine and other grade important diseases provide new medicine source selection, serve healthy cause.Meanwhile as one and mulberry tree garden resource
The industry to match, gold mushroom will become the distinctive new industry in Chunan County, and development prospect is good.
Specific embodiment
Below in conjunction with specific embodiment, the present invention is further explained：
The enforcement place of the present invention belongs to provincial ecological town, environment is very graceful, and base is far from Chunan in Chunan County Jie Shou townshiies seeds of a tung oil tree depressed place
The 0.2 hour range of driving in county town, traffic are very convenient.It is completed 2000 square metres of Standard Factory Room 2, edible mushroom Standard Factory Room 5000
Square metre, advanced cold chain machining production line 1, edible mushroom bacterium bag production line 2 and planting edible mushroom demonstration greenhouse 3000
Square metre.
Gold mushroom cultivation area selection of the present invention can form the production that resource matches near mulberry tree garden with mulberry tree garden
Industry.
Embodiment 1
A kind of gold mushroom cultivation method, the method includes selection cultivation season and place, selection planting material, cultivate strain,
Inoculation cultivation is with burying wood；
Bacteria condition be 23-25 DEG C of temperature, air humidity 60-65%；
The water content of culture medium is 60%；
Cultivation condition is 18-22 DEG C, air humidity 80-85%, light application time 6h/d；
Wherein cultivate the raw material that the culture medium used in strain includes following parts by weight：15 parts of cotton seed hulls, 20 parts of mulberry wood chips, 30 parts
Weed tree sawdust, 15 parts of wheat brans, 1 portion of corn flour, 1 part of lime and 3 parts of auxiliary materials.
The auxiliary material is that mass ratio is 3: 1: 1 active peptides calcium chelates of zinc, Rosmarinic acid and xanthyletin.
The preparation method of active peptides calcium chelates of zinc is：
A) it ferments：Active peptides are prepared with actinomycete fermentation；Fermentation step is：Weigh 33 parts of analysis for soybean powder, 10 parts of starch, yeast extract
7 parts, 15 parts of sucrose, 1 part of sodium chloride, 4 parts of peptone, 1 part of dipotassium hydrogen phosphate, 1 part of epsom salt, 20 parts of glucose, carbonic acid
3 parts of calcium, 1200 parts of distilled water, adjust pH7.2, and 112 DEG C of sterilizings obtain fermentation culture in 10 minutes；With fermentation culture quality
5% inoculation actinomyces starter, ferment 2 hours to obtain zymotic fluid under 28 DEG C of ventilation conditions；
B) it purifies：By centrifuging purification active peptides are purified with ammonium sulfate precipitation；Purification step is：Zymotic fluid is placed in centrifuge
In, it is centrifuged under the conditions of 4000r/min 10 minutes in 35 DEG C of temperature, takes supernatant；By supernatant at 4 DEG C refrigerated centrifuge,
Lower centrifugation 10 minutes, take supernatant under the conditions of rotating speed 7500r/min；Finely ground ammonium sulfate is added to according to 55% saturation degree
It in above-mentioned supernatant, is centrifuged 30 minutes with 9500r/min after being preserved overnight at 4 DEG C, the distilled water that precipitation adds in 2 times is taken to stir
Active peptides solution；
C) it chelates：Prepare active peptides calcium chelates of zinc；Chelation step is：The active peptides solution after purification is taken, under stiring
Add in the calcium chloride water containing 10% mass fraction and 1.1 times of zinc chloride water containing 7% mass fraction measured of 3 times of amounts
Solution adjusts pH as 7 using hydrochloric acid and sodium hydroxide solution, and concussion synthesis 35 minutes, obtain active peptides calcium zinc at a temperature of 20 DEG C
Chelate.
Embodiment 2
A kind of gold mushroom cultivation method, the method includes selection cultivation season and place, selection planting material, cultivate strain,
Inoculation cultivation is with burying wood；
Bacteria condition be 23-25 DEG C of temperature, air humidity 60-65%；
The water content of culture medium is 60%；
Cultivation condition is 18-22 DEG C, air humidity 80-85%, light application time 6h/d；
Wherein cultivate the raw material that the culture medium used in strain includes following parts by weight：20 parts of cotton seed hulls, 25 parts of mulberry wood chips, 32 parts
Weed tree sawdust, 18 parts of wheat brans, 3 portions of corn flour, 2 parts of lime and 5 parts of auxiliary materials.
The auxiliary material is that mass ratio is 3: 1: 1 active peptides calcium chelates of zinc, Rosmarinic acid and xanthyletin.
The preparation method of active peptides calcium chelates of zinc is：
A) it ferments：Active peptides are prepared with actinomycete fermentation；Fermentation step is：Weigh 33 parts of analysis for soybean powder, 10 parts of starch, yeast extract
7 parts, 15 parts of sucrose, 1 part of sodium chloride, 4 parts of peptone, 1 part of dipotassium hydrogen phosphate, 1 part of epsom salt, 20 parts of glucose, carbonic acid
3 parts of calcium, 1200 parts of distilled water, adjust pH7.3, and 113 DEG C of sterilizings obtain fermentation culture in 12 minutes；With fermentation culture quality
5% inoculation actinomyces starter, ferment 2-4 hours to obtain zymotic fluid under 29 DEG C of ventilation conditions；
B) it purifies：By centrifuging purification active peptides are purified with ammonium sulfate precipitation；Purification step is：Zymotic fluid is placed in centrifuge
In, it is centrifuged under the conditions of 4200r/min 12 minutes in 36 DEG C of temperature, takes supernatant；By supernatant refrigerated centrifuge at 5 DEG C,
Lower centrifugation 12 minutes, take supernatant under the conditions of rotating speed 7800r/min；Finely ground ammonium sulfate is added to according to 58% saturation degree
It in above-mentioned supernatant, is centrifuged 40 minutes with 9800r/min after being preserved overnight at 5 DEG C, the distilled water that precipitation adds in 3 times is taken to stir
Active peptides solution；
C) it chelates：Prepare active peptides calcium chelates of zinc；Chelation step is：The active peptides solution after purification is taken, under stiring
Add in the calcium chloride water containing 10% mass fraction and 1.2 times of zinc chloride containing 7% mass fraction measured of 3.2 times of amounts
Aqueous solution adjusts pH as 7-7.2 using hydrochloric acid and sodium hydroxide solution, and concussion synthesis 35 minutes, obtain active at a temperature of 20-22 DEG C
Polypeptide calcium chelates of zinc.
Embodiment 3
A kind of gold mushroom cultivation method, the method includes selection cultivation season and place, selection planting material, cultivate strain,
Inoculation cultivation is with burying wood；
Bacteria condition be 23-25 DEG C of temperature, air humidity 60-65%；
The water content of culture medium is 60%；
Cultivation condition is 18-22 DEG C, air humidity 80-85%, light application time 6h/d；
Wherein cultivate the raw material that the culture medium used in strain includes following parts by weight：25 parts of cotton seed hulls, 35 parts of mulberry wood chips, 35 parts
Weed tree sawdust, 22 parts of wheat brans, 5 portions of corn flour, 3 parts of lime and 7 parts of auxiliary materials.
The auxiliary material is that mass ratio is 3: 1: 1 active peptides calcium chelates of zinc, Rosmarinic acid and xanthyletin.
The preparation method of active peptides calcium chelates of zinc is：
A) it ferments：Active peptides are prepared with actinomycete fermentation；Fermentation step is：Weigh 33 parts of analysis for soybean powder, 10 parts of starch, yeast extract
7 parts, 15 parts of sucrose, 1 part of sodium chloride, 4 parts of peptone, 1 part of dipotassium hydrogen phosphate, 1 part of epsom salt, 20 parts of glucose, carbonic acid
3 parts of calcium, 1200 parts of distilled water, adjust pH7.4, and 114 DEG C of sterilizings obtain fermentation culture in 10-15 minutes；With fermentation culture quality
5% inoculation actinomyces starter, ferment under 30 DEG C of ventilation conditions 4 hours obtain zymotic fluid；
B) it purifies：By centrifuging purification active peptides are purified with ammonium sulfate precipitation；Purification step is：Zymotic fluid is placed in centrifuge
In, it is centrifuged under the conditions of 4500r/min 15 minutes in 38 DEG C of temperature, takes supernatant；By supernatant at 6 DEG C refrigerated centrifuge,
Lower centrifugation 15 minutes, take supernatant under the conditions of rotating speed 8000r/min；Finely ground ammonium sulfate is added to according to 60% saturation degree
It in above-mentioned supernatant, is centrifuged 45 minutes with 10000r/min after being preserved overnight at 6 DEG C, the distilled water stirring that precipitation is taken to add in 3 times
Obtain active peptides solution；
C) it chelates：Prepare active peptides calcium chelates of zinc；Chelation step is：The active peptides solution after purification is taken, under stiring
Add in the calcium chloride water containing 10% mass fraction and 1.4 times of zinc chloride containing 7% mass fraction measured of 4.5 times of amounts
Aqueous solution adjusts pH as 7-7.2 using hydrochloric acid and sodium hydroxide solution, and concussion synthesis 35 minutes, obtain active at a temperature of 20-22 DEG C
Polypeptide calcium chelates of zinc.
Gold mushroom has high medical value, and the polysaccharide body contained in the mucus on cap is to the inhibiting rate of tumour
80-90%, also containing a kind of nucleic acid, to human body, mental, energy has restitution in mucus.In addition eating gold mushroom can also be pre-
Anti- grape grape coccus, the infection of Escherichia coli, pneumobacillus and tubercle bacillus.Therefore, gold mushroom has anticancer, anti-inflammation, brain tonic, Qiang Ti
The effect of.Gold mushroom mushroom cooks delicious flavour, and unique flavor is in good taste.It is rich in protein, multivitamin and micro member
Element, it is indispensable containing human body in its protein and cannot itself synthesis must lean on the extraneous eight kinds of amino acid supplemented, symbol
Close that the United Nations's grain farmer's group (FAO) and the World Health Organization (WHO) should meet natural, nutrition about new food resource, health care is wanted
It asks, is rare to integrate medicine, food, health care and delicious shared rare mushroom.
It can be seen that the implementation of the present invention can provide high-quality green agricultural product for market, for peasant's increasing both production and income, and energy
Large-scale production and deep development based on Chunan County characteristic new medicinal resources utilize, and achievement in research may be oncotherapy, inflammation
Disease treatment, anti-bacterial vaccines and other etc. important diseases new medicine source selection is provided, serve healthy cause.Meanwhile as one
The industry to match with mulberry tree garden resource, gold mushroom will become the distinctive new industry in Chunan County, and development prospect is good.
By building and implementing, the packet of gold mushroom 50,000 can be produced year.Whole year achievable 2,800,000 yuan of income from sales, is realized net
560,000 yuan of profit.Safe and efficient standardized planting can carry out new opportunity to develop for Chunan County agricultural belt simultaneously, drive agricultural warp
The development of Ji.
Bacteria stick returning to the field makees fertilizer, improves the soil per acre using 2000 bags of waste bacterium rod, implements 60 mu of mulberry tree garden, passes through waste bacterium rod
Fertilizer Transformed utilize, 50,000 bags of obsolete fungus stick returning to the field, fertilizer cost by it is original per acre 178 yuan drop to 70 yuan or so, reduce base manure
Urea uses 2.8 tons (pure 1.32 tons of nitrogen).Meanwhile by the use of waste bacterium rod as organic fertilizer returning to the field, turn waste into wealth, not only reduce
Production cost, pine have changed soil, improve mulberry tree garden soil organic matter, and effectively improve local look of a village village looks.
By discard bacterium bag return recycle can 0.4 ten thousand yuan of cost-saving synergistic (useless 0.02 yuan of bacterium bag unit take-back cost/only, including
De- bag in production process outside damaged and bacterium bag).
Ramulus mori cultivation on sawdust is ground into mulberry branch, 11 tons or so of the dry branch of every ten thousand bags of need, make strain, bacteria stick needs altogether
55 tons of mulberry branch is wanted, to purchase in terms of 600 yuan/ton of mulberry branch, only this item just increases direct economic benefit for local sericulture peasant household
3.3 ten thousand yuan.
The utilization of mulberry tree garden resource is made to form a benign cycle using mulberry branch culturing edible fungus, medicinal fungus, it is real
Interdependence between plant (mulberry tree), fungus (medicinal fungus), the biologic chain relationship for influencing each other, mutually restricting are showed, have promoted
The ecological balance between nature biotechnology.Pine, weedtree cultivation gold mushroom are substituted using mulberry branch, carries out the artificial bacterium bag of scale
Cultivation research, can not only reduce the felling of forest tree resource and the wild gold mushroom germ plasm resource of effective protection, but also can rationally utilize
Mulberry branch is turned waste into wealth, comprehensive utilization.
Bacteria stick returning to the field improves soil, improves soil water-reataining fertilizer-keeping ability, and energy chesson, improves the photosynthetic work of crops
With mitigation crop diseases and pest harm.The recycling of discarded bacterium bag, avoids secondary pollution, has reached economic development and life
The harmony of state protection.
Claims (7)
1. a kind of gold mushroom cultivation method, which is characterized in that the method includes selection cultivation seasons and place, selection cultivation material
Material cultivates strain, inoculation cultivation with burying wood；Wherein cultivate the raw material that the culture medium used in strain includes following parts by weight：15-
25 parts of cotton seed hulls, 20-35 parts of mulberry wood chips, 30-35 parts of weed tree sawdusts, 15-22 parts of wheat brans, 1-5 portions of corn flour, 1-3 parts of lime and 3-7
Part auxiliary material.
2. a kind of gold mushroom cultivation method according to claim 1, which is characterized in that the auxiliary material is active peptides calcium
Chelates of zinc, Rosmarinic acid and xanthyletin.
A kind of 3. gold mushroom cultivation method according to claim 2, which is characterized in that the system of active peptides calcium chelates of zinc
Preparation Method is：
A) it ferments：Active peptides are prepared with actinomycete fermentation；Fermentation step is：Weigh 33 parts of analysis for soybean powder, 10 parts of starch, yeast extract
7 parts, 15 parts of sucrose, 1 part of sodium chloride, 4 parts of peptone, 1 part of dipotassium hydrogen phosphate, 1 part of epsom salt, 20 parts of glucose, carbonic acid
3 parts of calcium, 1200 parts of distilled water, adjust pH7.2-7.4, and 112-114 DEG C of sterilizing obtains fermentation culture in 10-15 minutes；With training of fermenting
5% inoculation actinomyces starter of nutrient solution quality, ferment 2-4 hours to obtain zymotic fluid under 28-30 DEG C of ventilation condition；
B) it purifies：By centrifuging purification active peptides are purified with ammonium sulfate precipitation；Purification step is：Zymotic fluid is placed in centrifuge
In, it is centrifuged under the conditions of 4000-4500r/min 10-15 minutes in 35-38 DEG C of temperature, takes supernatant；By supernatant at 4-6 DEG C
Lower refrigerated centrifuge, lower centrifugation 10-15 minutes, take supernatant under the conditions of rotating speed 7500-8000r/min；By finely ground ammonium sulfate
Saturation degree according to 55-60% is added in above-mentioned supernatant, is centrifuged after being preserved overnight at 4-6 DEG C with 9500-10000r/min
30-45 minutes, the distilled water that precipitation adds in 2-3 times is taken to stir to obtain active peptides solution；
C) it chelates：Prepare active peptides calcium chelates of zinc；Chelation step is：The active peptides solution after purification is taken, under stiring
Add in 3-4.5 times of calcium chloride water containing 10% mass fraction measured with 1.1-1.4 times measure containing 7% mass fraction
Solder(ing)acid adjusts pH as 7-7.2 using hydrochloric acid and sodium hydroxide solution, concussion synthesis 35 minutes at a temperature of 20-22 DEG C,
Obtain active peptides calcium chelates of zinc.
4. a kind of gold mushroom cultivation method according to claim 1, which is characterized in that bacteria condition is 23-25 DEG C of temperature,
Air humidity 60-65%.
5. a kind of gold mushroom cultivation method according to claim 1, which is characterized in that the water content of culture medium is 60%.
6. a kind of gold mushroom cultivation method according to claim 1, which is characterized in that Cultivation condition is 18-22 DEG C, air
Humidity 80-85%, light application time 6h/d.
A kind of 7. gold mushroom cultivation method according to claim 2, which is characterized in that the active peptides calcium zinc chelating
The mass ratio of object, Rosmarinic acid and xanthyletin is 3: 1: 1.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109076883A (en) * | 2018-08-28 | 2018-12-25 | 重庆市药物种植研究所 | A kind of liquid spawn-solid state cultivation kind cultural method of the rotten edible mushroom of grass |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103416224A (en) * | 2013-08-08 | 2013-12-04 | 刘万顺 | Method for preparing pleurotus geesteranus compost |
| CN103724115A (en) * | 2013-12-17 | 2014-04-16 | 广西柳城县成霖农业科技有限公司 | Lucid ganoderma pocket planting fungus bag |
| CN106665124A (en) * | 2017-01-18 | 2017-05-17 | 贵州省山地资源研究所有限公司 | Cultivation method for returning coriaria sinia mushroom to nature |
| CN106747848A (en) * | 2017-03-31 | 2017-05-31 | 徐振宗 | South Korea's Phellinus bacterium plastic bag cultivation method |
| CN107162676A (en) * | 2017-05-08 | 2017-09-15 | 张和平 | A kind of organic edible fungus culturing matrix and preparation method thereof |
| CN107318948A (en) * | 2017-07-06 | 2017-11-07 | 浦江县欧立生物技术有限公司 | The bio-preservative of hairtail |
-
2017
- 2017-11-29 CN CN201711232434.4A patent/CN108174741A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103416224A (en) * | 2013-08-08 | 2013-12-04 | 刘万顺 | Method for preparing pleurotus geesteranus compost |
| CN103724115A (en) * | 2013-12-17 | 2014-04-16 | 广西柳城县成霖农业科技有限公司 | Lucid ganoderma pocket planting fungus bag |
| CN106665124A (en) * | 2017-01-18 | 2017-05-17 | 贵州省山地资源研究所有限公司 | Cultivation method for returning coriaria sinia mushroom to nature |
| CN106747848A (en) * | 2017-03-31 | 2017-05-31 | 徐振宗 | South Korea's Phellinus bacterium plastic bag cultivation method |
| CN107162676A (en) * | 2017-05-08 | 2017-09-15 | 张和平 | A kind of organic edible fungus culturing matrix and preparation method thereof |
| CN107318948A (en) * | 2017-07-06 | 2017-11-07 | 浦江县欧立生物技术有限公司 | The bio-preservative of hairtail |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109076883A (en) * | 2018-08-28 | 2018-12-25 | 重庆市药物种植研究所 | A kind of liquid spawn-solid state cultivation kind cultural method of the rotten edible mushroom of grass |
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Application publication date: 20180619 |