CN102276708B - Method for continuously preparing protein, polysaccharide, amino acid, taurine and polypeptide from laver - Google Patents
Method for continuously preparing protein, polysaccharide, amino acid, taurine and polypeptide from laver Download PDFInfo
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- CN102276708B CN102276708B CN 201110222552 CN201110222552A CN102276708B CN 102276708 B CN102276708 B CN 102276708B CN 201110222552 CN201110222552 CN 201110222552 CN 201110222552 A CN201110222552 A CN 201110222552A CN 102276708 B CN102276708 B CN 102276708B
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Abstract
The invention provides a method for continuously preparing protein, polysaccharide, amino acid, taurine and polypeptide from laver. The method comprises the following steps: pulping the laver, and adding pure water into the laver according to a proportion of 1:(30-50) to prepare pulp; leading the pulp into an extracting process to prepare an extract; and continuously preparing laver protein, laver polysaccharide, amino acid substances, taurine, laver polypeptide and the like from the laver by utilizing an integrated membrane system after the extract passes through a prefilter device. Besides an enzyme preparation used when the polypeptide is prepared in a hydrolysis process, toxic or harmful chemical substances are not added in the whole producing process, and the whole producing process is a pure physical separation process.
Description
Technical field
The present invention relates to a kind of marine prods deep process technology field, particularly a kind of method of producing continuously laver albumen, laver amylose, amino acids material, taurine, laver polypeptide from laver.
Background technology
Laver belongs to the rhodophyta marine alga, as food and Chinese medicine, the applicating history of more than one thousand years has been arranged in China.China coast is extensively cultivated.The processing mode of laver is that it is made dry product at present, then makes the flaked product of seasoning, and is directly edible; Make flaked product, the wrap during as the making sushi; Make pancake shape product, be used for burning soup edible.The added value of these processing modes and technology content are very low.
Laver contains the nutritive substances such as rich in protein, polysaccharide, amino acids material, fat, inorganic salt.According to the different places of production and growth time, the dried laver of average every 100g contains protein 25%-35%, contains polysaccharide 15%-30%, contains amino acids material 29%-38%, and fatty 1.2%-3% contains inorganic salt 15%-20%.
Being widely used of protective foods, field of medicaments, the exploitation of laver Biological resources and research are become increasingly active due to the laver functional ingredient.The preparation for the laver functional component of report all concentrates on laver amylose and purple laver protein both at home and abroad at present, and yet there are no the research report for extraction separation and the preparation of functional substance taurine.
So far, the waste material after laver or its processing is as raw material, extracts continuously from laver and the technical matters of preparation major function composition laver albumen, polysaccharide, other amino acids materials, taurine, polypeptide there is not yet report both at home and abroad.In the correlation technique of extracting protein, polysaccharide from laver of having seen, without exception all add chemical substance in production process, be all much hazardous and noxious substances.
Summary of the invention
The technical problem to be solved in the present invention is for the deficiencies in the prior art, has proposed a kind of pollution-free, nontoxic method of producing continuously laver albumen, laver amylose, amino acids material, taurine, laver polypeptide from laver.
The technical problem to be solved in the present invention is achieved through the following technical solutions, and a kind of method of producing continuously laver albumen, laver amylose, amino acids material, taurine, laver polypeptide from laver is characterized in:
(1) selecting laver is raw material, after defibrination, adds pure water in the ratio of 1:30--50, makes slip;
(2) slip enters the lixiviate operation, temperature 60 C-80 ℃, and agitator speed 10-30r/min, lixiviate 6h-10h makes vat liquor;
(3) vat liquor enters pre-filtrating equipment, and the impurity such as filtering suspended substance are got back to the lixiviate operation by the impurity such as suspended substance of filtering, again lixiviate;
(4) vat liquor by after pre-filtrating equipment, enters the Ceramic Composite membrane filter plant of Integrated Films system, further removes impurity; Trapped impurity is got back to lixiviate operation, lixiviate again; Lixiviate residuum after the secondary lixiviate not as waste disposal, but as the raw material that extracts laver oil, is delivered to laver oil production plant and is used;
(5) by the vat liquor after the removal of impurities of Ceramic Composite membrane filter plant, enter in the ultra-filtration membrane separating device of Integrated Films system, molecular weight 1KD dams; Amino acids material and taurine enter holding tank No. 1, enter No. 2 holding tank after the macromolecular substance such as protein, polysaccharide are dammed;
Material in (6) No. 1 holding tanks enters reverse osmosis equipment, enters ion-exchange unit through the material after concentrated; Be separated into the solution that contains taurine and the solution that contains other amino acids by the material after ion-exchange; Finished product is made in the solution process refining step can of other amino acids; The solution that contains taurine enters the operations such as deposition and purification, drying, packing and makes finished product;
Material in (7) No. 2 holding tanks enters in another membrane separation plant of Integrated Films system, by the combining and configuring of the molecular weight 10KD-600KD film that dams, protein and separation of polysaccharides out; By the protein that membrane separation plant is separated, after the reverse osmosis concentration purifying, drying, packing are made finished product;
(8) the protein finished product that is produced take operation (7) is made the polypeptide finished product as raw material through combining and configuring system, reverse osmosis, drying, the packaging process of enzymolysis, the molecular weight 1K-30K dalton film that dams;
(9) by operation (7) membrane separation plant polysaccharide out, after the reverse osmosis concentration purifying, drying, packing are made finished product.
The technical problem to be solved in the present invention can also be come further realization by the following technical programs, and raw material is dried laver, fresh laver or the laver dregs of rice that extracted laver oil.
The technical problem to be solved in the present invention can also be come further realization by the following technical programs, and in operation (8) enzymolysis process, the enzyme amount is 1.0-3.0% of protein total mass, 30-45 ℃ of temperature, time 2-4h.
The present invention compared with prior art utilizes the Integrated Films system to produce continuously laver albumen, laver amylose, amino acids material, taurine, laver polypeptide etc. from laver.The zymin that will use when preparing polypeptide except hydrolyzing process, the present invention does not add toxic and harmful substance in whole production process, is the sepn process of a pure physical property.
Embodiment
A kind of method of producing continuously laver albumen, laver amylose, amino acids material, taurine, laver polypeptide from laver,
Concrete steps are as follows:
(1) select dried laver that pollution-free nothing is gone mouldy be raw material or with fresh laver after husky draining is removed in cleaning as raw material or take the laver residuum (also can be called the extraction residuum, also can be called the laver dregs of rice) that extracted laver oil as raw material.Raw material adds pure water in the ratio of 1:30--50 after defibrination, make slip.
(2) slip enters the lixiviate operation, temperature 60 C-80 ℃, and agitator speed 10-30r/min, lixiviate 6h-10h makes vat liquor.
(3) vat liquor enters pre-filtrating equipment, and the impurity such as filtering suspended substance are to be more conducive to Integrated Films system long-term operation.The impurity such as suspended substance by filtering are got back to the lixiviate operation, again lixiviate.
(4) vat liquor by after pre-filtrating equipment, enters the Ceramic Composite membrane filter plant of Integrated Films system, further removes impurity.Trapped impurity is got back to the lixiviate operation, lixiviate again, and the lixiviate residuum after the secondary lixiviate not as waste disposal, but as the raw material that extracts laver oil, is delivered to laver oil production plant and is used.
(5) by after the Ceramic Composite membrane filter plant, enter in the ultra-filtration membrane separating device of Integrated Films system, molecular weight 1KD dams.Amino acids material and taurine enter holding tank No. 1.After being dammed, the macromolecular substance such as protein, polysaccharide enters holding tank No. 2.
Material in (6) No. 1 holding tanks enters reverse osmosis equipment, enters ion-exchange unit through the material after concentrated; Be separated into the solution that contains taurine and the solution that contains other amino acids by the material after ion-exchange; Finished product is made in the solution process refining step can of other amino acids; The solution that contains taurine enters the operations such as deposition and purification, drying, packing and makes finished product.
Material in (7) No. 2 holding tanks enters in another membrane separation plant of Integrated Films system, by the combining and configuring of the molecular weight 10KD-600KD film that dams, protein and separation of polysaccharides out; By the protein that membrane separation plant is separated, after the reverse osmosis concentration purifying, drying, packing are made finished product.
(8) the protein finished product that is produced take operation (7) is made the polypeptide finished product as raw material through combining and configuring system, reverse osmosis, drying, the packaging process of enzymolysis, the molecular weight 1K-30K dalton film that dams.In enzymolysis process, the enzyme amount is 1.0-3.0% of protein total mass, 30-45 ℃ of temperature, time 2-4h.
(9) by operation (7) membrane separation plant polysaccharide out, after the reverse osmosis concentration purifying, drying, packing are made finished product.
The present invention is further elaborated below in conjunction with specific embodiment, but embodiment is not construed as limiting the invention.
Get the pollution-free dried laver 100kg that goes mouldy, add the pure water defibrination, cross aperture 100 μ m sieves, add pure water in the ratio of 1:50 and make slip; Slip enters extraction equipment, 60 ℃ of extraction temperatures, and extraction time 6h, agitator speed 20r/min makes vat liquor; Vat liquor enters pre-filtrating equipment, and the particle diameter that dams is greater than 10 μ m impurity, and impurity is got back to extraction equipment and carried out the secondary lixiviate, and clear liquid enters ceramic membrane equipment; Clear liquid enters ceramic membrane equipment laggard one and walks out of decon, and impurity is got back to extraction equipment and carried out the secondary lixiviate, and clear liquid enters the film separating system of Integrated Films system; The molecular weight 1KD that dams of film separating system, system feeding pressure 0.4Mpa, 40 ℃ of temperature, isolated amino acid and taurine mixture enter holding tank No. 1, and isolated protein and polysaccharide mixture enter holding tank No. 2; The amino acid of No. 1 holding tank and taurine mixture enter reverse osmosis equipment, and after concentrating 5 times, upper cationic resin column is separated, resin column is two series connection, and isolated taurine adds 4 times of volume dehydrated alcohol deposition and purifications, pass through again spraying drying, make finished product after packing; Contain other amino acid whose liquid through what resin column was separated, through after refining step, make liquid end product.Protein and polysaccharide mixture in No. 2 holding tanks enter another membrane separation plant, this membrane separation plant is the combining and configuring system of molecular weight 10KD-600KD of damming, separate through protein after this system and glycocalix, polysaccharide enters reverse osmosis equipment, makes the polysaccharide finished product through purifying, drying, packing.Protein enters another reverse osmosis equipment, makes the protein finished product through purifying, drying, packing.Part protein finished product enters hydrolyzing process, adopts mixed enzyme to process, and the enzyme amount is 1.5% of total protein, 35 ℃ of temperature, and time 3h after enzymolysis, through another film separating system and reverse osmosis isolation purifying, then through super-dry, packing, makes the polypeptide finished product.Produce wet filtering thing 45kg everywhere, deliver to laver oil production plant as raw material and use.
Claims (2)
1. method of producing continuously albumen, polysaccharide, amino acid, taurine, polypeptide from laver is characterized in that:
(1) selecting laver is raw material, after defibrination, adds pure water in the ratio of 1:30-50, makes slip;
(2) slip enters the lixiviate operation, temperature 60 C-80 ℃, and agitator speed 10-30r/min, lixiviate 6h-10h makes vat liquor;
(3) vat liquor enters pre-filtrating equipment, and the impurity such as filtering suspended substance are got back to the lixiviate operation by the impurity such as suspended substance of filtering, again lixiviate;
(4) vat liquor by after pre-filtrating equipment, enters the Ceramic Composite membrane filter plant of Integrated Films system, further removes impurity; Trapped impurity is got back to lixiviate operation, lixiviate again;
(5) by the vat liquor after the removal of impurities of Ceramic Composite membrane filter plant, enter in the ultra-filtration membrane separating device of Integrated Films system, adopt the daltonian film of molecular weight 1K that dams; Amino acids material and taurine enter holding tank No. 1, enter No. 2 holding tank after the macromolecular substance such as protein, polysaccharide are dammed;
Material in (6) No. 1 holding tanks enters reverse osmosis equipment, enters ion-exchange unit through the material after concentrated; Be separated into the solution that contains taurine and the solution that contains other amino acids by the material after ion-exchange; Finished product is made in the solution process refining step can of other amino acids; The solution that contains taurine enters deposition and purification, drying, packaging process and makes finished product;
Material in (7) No. 2 holding tanks enters in another membrane separation plant of Integrated Films system, by the combining and configuring of the daltonian film of molecular weight 10K-600K that dams, protein and separation of polysaccharides out; By the protein that membrane separation plant is separated, after the reverse osmosis concentration purifying, drying, packing are made finished product;
(8) the protein finished product that is produced take operation (7) is made the polypeptide finished product as raw material through combining and configuring filtering system, reverse osmosis, drying, the packaging process of enzymolysis, the daltonian film of molecular weight 1K-30K that dams;
(9) by operation (7) membrane separation plant polysaccharide out, after the reverse osmosis concentration purifying, drying, packing are made finished product;
In operation (8) enzymolysis process, the enzyme amount is 1.0-3.0% of protein total mass, 30-45 ℃ of temperature, time 2-4h.
2. the method for producing continuously albumen, polysaccharide, amino acid, taurine, polypeptide from laver according to claim 1, it is characterized in that: raw material is dried laver, fresh laver or the laver dregs of rice that extracted laver oil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110222552 CN102276708B (en) | 2011-08-04 | 2011-08-04 | Method for continuously preparing protein, polysaccharide, amino acid, taurine and polypeptide from laver |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201110222552 CN102276708B (en) | 2011-08-04 | 2011-08-04 | Method for continuously preparing protein, polysaccharide, amino acid, taurine and polypeptide from laver |
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| CN102276708A CN102276708A (en) | 2011-12-14 |
| CN102276708B true CN102276708B (en) | 2013-06-12 |
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| CN 201110222552 Expired - Fee Related CN102276708B (en) | 2011-08-04 | 2011-08-04 | Method for continuously preparing protein, polysaccharide, amino acid, taurine and polypeptide from laver |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102643368B (en) * | 2012-05-07 | 2014-04-09 | 连云港职业技术学院 | Method for synchronously extracting taurine, chitin and polypeptide from shrimp heads and shrimp leftovers |
| CN102702038B (en) * | 2012-06-15 | 2014-04-30 | 南京农业大学 | Method for extracting natural taurine from nori |
| CN104293871A (en) * | 2014-10-11 | 2015-01-21 | 江南大学 | Method for preparing porphyra anti-oxidation peptide and comprehensively utilizing byproducts |
| CN104628823B (en) * | 2015-02-09 | 2017-10-20 | 福州大学 | One main laver antioxidation polypeptide and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1796405B (en) * | 2004-12-27 | 2010-11-17 | 上海水产大学 | Method for separating and purifying phycobiliprotein in high purity from laver |
| CN1872882B (en) * | 2005-05-30 | 2010-05-26 | 上海水产大学 | Method for preparing laver amylose |
| CN102001974B (en) * | 2010-09-20 | 2013-09-25 | 连云港金海地食品有限公司 | Process for extracting and preparing taurine from laver |
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