CN101473979B - Method for extracting trepang oil from boiled trepang juice - Google Patents
Method for extracting trepang oil from boiled trepang juice Download PDFInfo
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Abstract
The invention discloses a method for extracting sea cucumber oil from sea cucumber cooking liquor. The method comprises the steps as follows: 1. the separation of cooking liquor; 2. ultrafiltration, concentration and desalination; 3. sterilization and zymohydrolysis; 4. inactivation of enzyme; 5. centrifugal filtration; 6. triple effect concentration; 7. deposition in alcohol; 8. secondary desalination; and 9. distillation for recycling the sea cucumber oil, so as to obtain the sea cucumber oil in the sea cucumber cooking liquor finally. The sea cucumber oil contains omega-3 series polyunsaturated fatty acids and contains main components of eicosapentaenoic acid (EPA for short), docosahexenoic acid (DHA for short) and a small quantity of salt. The method not only can extract the sea cucumber oil with high nutrition value, can be widely applied in the fields of food, health care and the like, but also can reduce the environmental pollution after the emission of the sea cucumber cooking liquor and has obvious economic and social benefits.
Description
Technical field
The present invention relates to a kind of sea cucumber oil substances method of (being called for short sea cucumber oil) of from boiled trepang juice, extracting, belong to the technical field of marine product deep processing.
Background technology
The fish oil that from abyssal fishes, extracts at present has certain research, and the fish oil of abyssal fishes contains the physiological activator that human health and intelligence development is had significant impact, i.e. the serial polyunsaturated fatty acid of ω-3.(English name is Eicosapentaenoic acid to its key component eicosapentaenoic acid; Be called for short EPA) and DHA (English name is Docosahexaenoic acid; Be called for short DHA); Not only in the structure of retina and brain, play an important role, but also be the attemperator that eicosatetraenoic acid is metabolized to arachidic acid.In view of the important value of this type material, countries in the world government all takes much count of the food development that is rich in EPA, DHA.
Sea cucumber is as the profuse marine prods of a kind of nutritive value; Deep processing research and application to it at present mostly concentrates on fields such as sea cucumber polysaccharide, sea cucumber saponin, sea cucumber polypeptide; And it is refined the research that unrighted acid promptly is commonly called as sea cucumber oil; Still blank both at home and abroad at present, relevant report is not seen in the research that particularly utilizes the discarded liquor that produces in the sea cucumber process to carry out sea cucumber oil especially.
At present the technology in sea cucumber deep processing field has: the patent No. is the preparation method that Chinese invention patent that CN 031119255, name are called " preparation method of sea cucumber polysaccharide " discloses a kind of sea cucumber polysaccharide; It is by means of the effect of the autolytic enzyme of fresh and alive sea cucumbers self; Be aided with an amount of natural biological and make degradation product, in conjunction with the method that has adopted physics catalysis, without concentrating, do not add any poisonous and hazardous chemical solvent; Changed and added the chemical degradation thing in the past and degrade; Remove the chemical degradation thing again Deng after the degraded, add acid again or add the complicated technology method that alkali carries out the adjustment of pH value, adopted prepared sea cucumber polysaccharides such as direct ultra-filtration is held back, post purifying, washing; The Chinese invention patent application that application number is 2006101462022, name is called " utilizing boiled trepang juice to extract the method for various active compositions such as sea cucumber polysaccharide " discloses a kind of method of utilizing boiled trepang juice to extract various active compositions such as sea cucumber polysaccharide; Its utilization is fished for natural sea cucumber or is propagated the liquor of sea cucumber in be processed into dried sea-cucumber and other product digestion process by bright sea cucumber artificially; Or the liquor of endoceliac sea cucumber intestine of bright sea cucumber and sexual gland, through complex enzyme zymohydrolysis, ethanol purification, centrifugation, filtration, concentrate, technology such as freeze drying processes from boiled trepang juice and extracts sea cucumber polysaccharide, sea cucumber saponin, sea cucumber polypeptide isoreactivity nutriment.But these are technological or utilize fresh and alive sea cucumbers or boiled trepang juice to extract materials such as sea cucumber polysaccharide, sea cucumber saponin, sea cucumber polypeptide, do not relate to the extraction of sea cucumber oil at all.
Summary of the invention
Be worth in order to improve the sea cucumber deep processing and utilization, realize the recycling of boiled trepang juice, the present invention provides a kind of method of from boiled trepang juice, extracting the sea cucumber oil component on known boiled trepang juice reutilization technology basis.
The present invention includes following content:
With the liquor that produces in the sea cucumber process is raw material:
Step 1: liquor separates---and adopt 100 mesh filter screens to carry out coarse filtration boiled trepang juice, the filtrating that will collect again is passed in the centrifuge and centrifugalizes, and the filtrating after the centrifugation is collected subsequent use; It is characterized in that: centrifuge charging limit the quantity of for 135kg, maximum speed 1200r/min, used filter cloth be 250 orders.
Step 2: ultrafiltration concentrating and desalinating---the filtrating that will collect step 1 is squeezed into the nanofiltration desalination that circulates in the ultra micro membrane separation plant with concentrated; It is characterized in that: the polyamide composite film that NF membrane adopts peace moral membrane separation technique Engineering Co., Ltd to produce, the aperture is several nanometers, molecular cut off is lower than till 0.5% until salt concentration at the molecule more than 200.
Step 3: sterilization, enzymolysis---step 2 ultrafiltration trapped fluid squeezed into sterilize in the activation pot and enzymolysis; It is characterized in that: boil and kept 30-40 minute, be cooled to 40-50 ℃ and keep this temperature then, regulate pH value, in every kilogram of liquor filtrating, add 8-15g effectively hydrolyzing protease, enzymolysis 2-3 hour at 7.0-8.0.
Step 4: enzyme goes out---with the step 3 enzymolysis liquid enzyme that in activation pot, goes out; It is characterized in that: the liquor behind the enzymolysis of step 3 is heated to 75-85 ℃, keeps 15-20 minute.
Step 5: centrifugal filtration: the liquor of step 4 squeezed in the 3-foot filter centrifugal once more filter, collect filtrating; It is characterized in that: more than the centrifuge maximum speed 1200r/min, used filter cloth is 250 orders.
Step 6: triple effect concentrates---and carry out triple effect in the trapped fluid feeding triple effect concentrator with step 5 and concentrate; It is characterized in that: controlled condition is the steam of 800-1000kg/h, 0.07-0.09MPa, and an effect parameter is 70-80 ℃, 0.04MPa; Two effect parameters are 60-70 ℃, 0.06MPa, and the triple effect parameter is 50-60 ℃, 0.08MPa, the liquor proportion 1.10-1.20 after concentrating.
Step 7: alcohol precipitation---the paste of the thickness after step 6 concentrated concentrates liquor and carries out precipitation with alcohol, in inspissated juice, adds edible ethanol and makes concentration of alcohol reach 80%-85%, leaves standstill after adding 12 hours; It is characterized in that: the edible ethanol concentration of adding is 90-95%, and the limit slowly adds the limit and stirs, guarantees to stir.
Step 8: desalination once more---pour out supernatant earlier after the liquor of step 7 left standstill, residue is poured desalination in the link-suspended basket centrifuge into; It is characterized in that: the centrifuge maximum speed is more than the 1200r/min, and filter cloth 250 orders centrifugalize and survey salt after 5 minutes, and salinity is higher than 1% continuation and carries out alcohol precipitation with the edible ethanol of 80%-85%, is lower than till 1% up to salinity.
Step 9: distill and reclaim sea cucumber oil---the ethanol of each time alcohol precipitation collection is squeezed in the multifunctional alcohol recovery inspissator carried out the alcohol distillation recovery, the alcohol of recovery is after alcohol meter is measured, and concentration is higher than 85% and is re-used in the alcohol precipitation process.To remain thick liquid after alcohol reclaims collects; Again thick liquid was cooled off 5-10 hour under 0 ℃ to-10 ℃ environment; After the cooling solid mass is pulled out; Add and boil 5-30 minute in the entry, constantly stir in the boiling part block oily solid leached to collect to be and contain the serial polyunsaturated fatty acid of ω-3, key component is that the sea cucumber of eicosapentaenoic acid (being called for short EPA), DHA (being called for short DHA) and a small amount of salinity is oily.It is characterized in that: alcohol reclaims the pressure of inspissator at 0.05-0.09MPa, and vacuum is 0.03-0.08MPa, and recovered alcohol sinks liquid 200kg/h, steam consumption quantity 1.9kg/h; The thick liquid cooling condition is 0 ℃ to-10 ℃, and be 5-10 hour cool time; Solid mass is 1 with the part by weight that adds water: 5-1: 20; Boiling time is 5-30 minute.
Raw material involved in the present invention is the usual commodity of using of food industry, all can on market, buy.Wherein, the hydrolysising protease that relates to is the product of Wuxi snow plum enzyme preparation Science and Technology Ltd., and market is buied.Used ethanol is the satisfactory edible alcohol of concentration.
The present invention does not relate to special equipment, except that having spelt out, is industry common equipment.Wherein, NF membrane adopts the polyamide composite film that peace moral membrane separation technique Engineering Co., Ltd produces.
Detection method involved in the present invention is the known common method of the industry.
The boiled trepang juice that the present invention extracts sea cucumber oil from boiled trepang juice method will be regarded as waste liquid is used; The sea cucumber oil component of its extraction is the serial polyunsaturated fatty acid of ω-3, and key component is eicosapentaenoic acid (being called for short EPA), DHA (being called for short DHA) and a small amount of salinity.
The present invention extracts the method for sea cucumber oil from boiled trepang juice, the sediment that behind the step 8 desalination, obtains also can obtain other sea cucumber active components after drying.
The present invention extracts the method for sea cucumber oil from boiled trepang juice, the sea cucumber oil that not only extracts is of high nutritive value, and can be widely used in fields such as food, health care, also can reduce boiled trepang juice discharging back environmental pollution simultaneously, has tangible economic and social benefit.
The specific embodiment
Below in conjunction with embodiment to further explain of the present invention.
Embodiment 1
Use method of the present invention, concrete operations are following:
1. adopt 100 mesh filter screens to carry out coarse filtration boiled trepang juice, the filtrating that will collect again is passed in the centrifuge and centrifugalizes.The centrifuge charging is limited the quantity of to 135kg, maximum speed 1200r/min, used filter cloth are 250 orders, and the filtrating after the centrifugation is collected subsequent use.
2. the filtrating that will collect is squeezed into the nanofiltration concentrating and desalinating that circulates in the ultra micro membrane separation plant; The polyamide composite film that NF membrane adopts peace moral membrane separation technique Engineering Co., Ltd to produce; The aperture is several nanometers; Molecular cut off concentrates with desalination and carries out synchronously at the molecule more than 200, is lower than till 0.5% up to salt concentration.
3. the ultrafiltration trapped fluid is squeezed into and boiled in the activation pot and keep sterilizing in 30 minutes; Be cooled to 40 ℃ and keep this temperature then; Regulate pH value 7.0, in every kilogram of liquor filtrating, add the effectively hydrolyzing protease that 8g purchases the snow plum enzyme preparation Science and Technology Ltd. in the Wuxi, enzymolysis 3 hours.
4. the intact back of enzymolysis is warming up to 75 ℃ with liquor in activation pot, keeps 15 minutes, and enzyme goes out.
5. centrifugal filtration: with behind the enzymolysis and the liquor of enzyme of having gone out squeeze into once more in the 3-foot filter centrifugal and filter, maximum speed 1200r/min, used filter cloth are 250 orders, collect filtrating.
6. carry out triple effect in the trapped fluid feeding triple effect concentrator after will filtering and concentrate, controlled condition is the steam of 800kg/h, 0.07MPa.Its triple effect parameter is: one imitates: 80 ℃, 0.04MPa; Two imitate 70 ℃, 0.06MPa; Triple effect: 60 ℃, 0.08MPa, the liquor proportion 1.10 after concentrating.
7. the paste of the thickness after will concentrating concentrates liquor, carries out precipitation with alcohol, and the edible ethanol of adding 90% makes concentration of alcohol reach 80% in inspissated juice, and the limit slowly adds the limit to be stirred, guarantee to stir, and leaves standstill after adding 12 hours.Add amount of alcohol by computes:
L＝VC2/(C2-C1)
In the formula, V is the volume of inspissated juice, and V=W/d, W are soup weight, and d is the density of soup; C1 is for being added concentration of alcohol, and C2 reaches concentration of alcohol for intending.
8. pour out supernatant after alcohol precipitation leaves standstill earlier, then remaining mixture is poured in the link-suspended basket centrifuge, rotating speed is 1200r/min, and 250 order filter clothes centrifugalized after 5 minutes, measures the salinity that filters the gained deposition.Salinity is higher than 1% continuation and carries out alcohol precipitation with 80% edible ethanol, stirs while adding evenly, and salinity is surveyed in centrifugation then, is lower than till 1% up to salinity.
9. the ethanol of each time alcohol precipitation being collected is squeezed into multifunctional alcohol and is reclaimed in the inspissator; Working pressure is at 0.05MPa, and useful vacuum degree is 0.03MPa, and recovered alcohol sinks liquid 200kg/h; Steam consumption quantity 1.9kg/h; Carry out alcohol distillation and reclaim, the alcohol of recovery is after alcohol meter is measured, and concentration is higher than 85% and is re-used in the alcohol precipitation process; To remain thick liquid after alcohol reclaims cooled off 5 hours under 0 ℃ of environment; After the cooling solid mass is pulled out; Add in solid mass and 1: 5 ratio of water and to boil 5 minutes in the entry; Constantly stir in the boiling part, in the stirring block oily solid leached to collect to be and contain the serial polyunsaturated fatty acid of ω-3, key component is that the sea cucumber of eicosapentaenoic acid (being called for short EPA), DHA (being called for short DHA) and a small amount of salinity is oily.
Embodiment 2
Use method of the present invention, concrete operations are following:
1. adopt 100 mesh filter screens to carry out coarse filtration boiled trepang juice, the filtrating that will collect again is passed in the centrifuge and centrifugalizes.The centrifuge charging is limited the quantity of to 135kg, maximum speed 1200r/min, used filter cloth are 250 orders, and the filtrating after the centrifugation is collected subsequent use.
2. the filtrating that will collect is squeezed into the nanofiltration concentrating and desalinating that circulates in the ultra micro membrane separation plant; The polyamide composite film that NF membrane adopts peace moral membrane separation technique Engineering Co., Ltd to produce; The aperture is a nanoscale; The molecule of molecular cut off more than 200 concentrates with desalination and carries out synchronously, is lower than till 0.5% up to salt concentration.
3. the ultrafiltration trapped fluid is boiled and keep sterilizing in 35 minutes; Be cooled to 45 ℃ and keep this temperature then; Regulate pH value 7.5, in every kilogram of liquor filtrating, add the effectively hydrolyzing protease that 12g purchases the snow plum enzyme preparation Science and Technology Ltd. in the Wuxi, enzymolysis 2.5 hours.
4. enzymolysis is warming up to 85 ℃ with liquor after finishing, and keeps 18 minutes, and enzyme goes out.
5. centrifugal filtration: with behind the enzymolysis and the liquor of enzyme of having gone out squeeze into once more in the 3-foot filter centrifugal and filter, maximum speed 1200r/min, used filter cloth are 250 orders, collect filtrating.
6. carry out triple effect in the trapped fluid feeding triple effect concentrator after will filtering and concentrate, controlled condition is the steam of 900kg/h, 0.08MPa.Concentrated parameter is: one imitates: 70 ℃, 0.04MPa; Two imitate 60 ℃, 0.06MPa; Triple effect: 50 ℃, 0.07MPa, the liquor proportion 1.15 after concentrating.
7. the paste of the thickness after will concentrating concentrates liquor, carries out precipitation with alcohol, and the edible ethanol of adding 95% makes concentration of alcohol reach 83% in inspissated juice, and the limit slowly adds the limit to be stirred, guarantee to stir, and leaves standstill after adding 12 hours.Add amount of alcohol by computes:
L＝VC2/(C2-C1)
In the formula, V is the volume of inspissated juice, and V=W/d, W are soup weight, and d is the density of soup; C1 is for being added concentration of alcohol, and C2 reaches concentration of alcohol for intending.
8. pour out supernatant after alcohol precipitation leaves standstill earlier, then remaining mixture is poured in the link-suspended basket centrifuge, rotating speed is 1200r/min, and 250 order filter clothes centrifugalized after 5 minutes, measures the filtration gained deposition salinity of collecting.Salinity is higher than 1% continuation and carries out alcohol precipitation with 85% edible ethanol, stirs while adding evenly, and salinity is surveyed in centrifugation then, is lower than till 1% up to salinity.
9. the ethanol of each time alcohol precipitation being collected is squeezed into multifunctional alcohol and is reclaimed in the inspissator; Working pressure is at 0.07MPa, and useful vacuum degree is 0.06MPa, and recovered alcohol sinks liquid 200kg/h; Steam consumption quantity 1.9kg/h; Carry out alcohol distillation and reclaim, the alcohol of recovery is after alcohol meter is measured, and concentration is higher than 85% and is re-used in the alcohol precipitation process; To remain thick liquid after alcohol reclaims cooled off 8 hours under-5 ℃ of environment; After the cooling solid mass is pulled out; Add in solid mass and 1: 15 ratio of water and to boil 15 minutes in the entry; Constantly stir in the boiling part, in the stirring block oily solid leached to collect to be and contain the serial polyunsaturated fatty acid of ω-3, key component is that the sea cucumber of eicosapentaenoic acid (being called for short EPA), DHA (being called for short DHA) and a small amount of salinity is oily.
Embodiment 3
Concrete operations are following:
1. adopt 100 mesh filter screens to carry out coarse filtration boiled trepang juice, the filtrating that will collect again is passed in the centrifuge and centrifugalizes.The centrifuge charging is limited the quantity of to 135kg, maximum speed 1200r/min, used filter cloth are 250 orders, and the filtrating after the centrifugation is collected subsequent use.
2. the filtrating that will collect is squeezed into the nanofiltration concentrating and desalinating that circulates in the ultra micro membrane separation plant; The polyamide composite film that NF membrane adopts peace moral membrane separation technique Engineering Co., Ltd to produce; The aperture is several nanometers; Molecular cut off concentrates with desalination and carries out synchronously at the molecule more than 200, and salt concentration is lower than till 0.5%.
3. the ultrafiltration trapped fluid is squeezed into and boiled in the activation pot and keep sterilizing in 40 minutes; Be cooled to 50 ℃ and keep this temperature then; Regulate pH value 8.0, in every kilogram of liquor filtrating, add the effectively hydrolyzing protease that 15g purchases the snow plum enzyme preparation Science and Technology Ltd. in the Wuxi, enzymolysis 3.0 hours.
4. the intact back of enzymolysis is warming up to 85 ℃ with liquor in activation pot, keeps 20 minutes, and enzyme goes out.
5. centrifugal filtration: with behind the enzymolysis and the liquor of enzyme of having gone out squeeze into once more in the 3-foot filter centrifugal and filter, maximum speed 1200r/min, used filter cloth are 250 orders, collect filtrating.
6. carry out triple effect in the trapped fluid feeding triple effect concentrator after will filtering and concentrate, controlled condition is the steam of 1000kg/h, 0.09MPa.Concentrated parameter is: one imitates: 75 ℃, 0.04MPa; Two imitate 65 ℃, 0.06MPa; Triple effect: 55 ℃, 0.07MPa, the liquor proportion 1.20 after concentrating.
7. the paste of the thickness after will concentrating concentrates liquor, carries out precipitation with alcohol, and the edible ethanol of adding 95% makes concentration of alcohol reach 85% in inspissated juice, and the limit slowly adds the limit to be stirred, guarantee to stir, and leaves standstill after adding 12 hours.Add amount of alcohol by computes:
L＝VC2/(C2-C1)
In the formula, V is the volume of inspissated juice, and V=W/d, W are soup weight, and d is the density of soup; C1 is for being added concentration of alcohol, and C2 reaches concentration of alcohol for intending.
8. pour out supernatant after alcohol precipitation leaves standstill earlier, then remaining mixture is poured in the link-suspended basket centrifuge, rotating speed is 1200r/min, and 250 order filter clothes centrifugalized after 8 minutes, measures the filtration gained deposition salinity of collecting.Salinity is higher than 1% continuation and carries out alcohol precipitation with 85% edible ethanol, stirs while adding evenly, and salinity is surveyed in centrifugation then, is lower than till 1% up to salinity.
9. the ethanol of each time alcohol precipitation being collected is squeezed into multifunctional alcohol and is reclaimed in the inspissator; Working pressure is at 0.09MPa, and useful vacuum degree is 0.08MPa, and recovered alcohol sinks liquid 200kg/h; Steam consumption quantity 1.9kg/h; Carry out alcohol distillation and reclaim, the alcohol of recovery is after alcohol meter is measured, and concentration is higher than 85% and is re-used in the alcohol precipitation process; To remain thick liquid after alcohol reclaims cooled off 10 hours under-10 ℃ of environment; After the cooling solid mass is pulled out; Add in solid mass and 1: 20 ratio of water and to boil 30 minutes in the entry; Constantly stir in the boiling part, in the stirring block oily solid leached to collect to be and contain the serial polyunsaturated fatty acid of ω-3, key component is that the sea cucumber of eicosapentaenoic acid (being called for short EPA), DHA (being called for short DHA) and a small amount of salinity is oily.
The boiled trepang juice that the present invention extracts sea cucumber oil from boiled trepang juice method will be regarded as waste liquid is used; The sea cucumber oil component of its extraction has the serial polyunsaturated fatty acid of ω-3, and key component is the sea cucumber oil of eicosapentaenoic acid (being called for short EPA), DHA (being called for short DHA) and a small amount of salinity.
The present invention extracts the method for sea cucumber oil from boiled trepang juice, the sediment that behind the step 8 desalination, obtains also can obtain other sea cucumber active components after drying.
The present invention extracts the method for sea cucumber oil from boiled trepang juice, the sea cucumber oil that not only extracts is of high nutritive value, and can be widely used in fields such as food, health care; Also can reduce boiled trepang juice discharging back environmental pollution simultaneously, have tangible economic and social benefit.
Claims (1)
1. one kind is extracted the oily method of sea cucumber from boiled trepang juice, and it passes through following processing step:
(1) liquor separates, and adopts 100 mesh filter screens to carry out coarse filtration boiled trepang juice, and the filtrating that will collect again is passed in the centrifuge and centrifugalizes, and the filtrating after the centrifugation is collected subsequent use; The charging of centrifuge is limited the quantity of and is that 135kg, maximum speed 1200r/min, used filter cloth are 250 orders;
(2) ultrafiltration concentrating and desalinating; The filtrating of collecting is squeezed into the nanofiltration that circulates in the ultra micro membrane separation plant to be concentrated and desalination; The polyamide composite film that NF membrane adopts peace moral membrane separation technique Engineering Co., Ltd to produce, the aperture of polyamide composite film is a nanoscale, molecular cut off is at the molecule more than 200; Concentrate with desalination and carry out synchronously, be lower than till 0.5% until salt concentration;
(3) the sterilization enzymolysis is squeezed into the trapped fluid behind the ultrafiltration concentrating and desalinating in the activation pot, boils and keeps 30-40 minute; Be cooled to 40-50 ℃ and keep this temperature then; Regulate the pH value at 7.0-8.0, in every kilogram of liquor filtrating, add 8-15g effectively hydrolyzing protease, enzymolysis 2-3 hour;
(4) enzyme that goes out with the enzymolysis liquid enzyme that in activation pot, goes out, when going out enzyme is heated the liquor behind the enzymolysis to 75-85 ℃, keeps 15-20 minute;
(5) centrifugal filtration, with behind the enzymolysis and the liquor of enzyme of having gone out squeeze in the 3-foot filter centrifugal and filter, collect filtrating, the centrifuge maximum speed is 1200r/min, used filter cloth is 250 orders;
(6) triple effect concentrates; Concentrate carrying out triple effect in the trapped fluid feeding triple effect concentrator after filtering; Controlled condition is the steam of 800-1000kg/h, 0.07-0.09MPa, and an effect parameter is 70-80 ℃, 0.04MPa, and two effect parameters are 60-70 ℃, 0.06MPa; The triple effect parameter is 50-60 ℃, 0.08MPa, the liquor proportion 1.20-1.30 after concentrating;
(7) alcohol precipitation carries out precipitation with alcohol with the concentrated liquor of the paste of the thickness after concentrating, and in concentrating liquor, adds edible ethanol and makes concentration of alcohol reach 80%-85%; Left standstill after adding 12 hours; The edible ethanol concentration that adds is 90%-95%, and the limit slowly adds the limit stirs, and guarantees to stir;
(8) desalination once more; Liquor behind the alcohol precipitation is poured out supernatant, pour the remaining mixture of pouring out supernatant in link-suspended basket centrifuge desalination, the centrifuge maximum speed is 1200r/min; Filter cloth 250 orders; Centrifugalize and survey salt after 5 minutes, salinity is higher than the edible ethanol continuation alcohol precipitation of 1% continuation with 80%-85%, is lower than till 1% up to salinity;
(9) distill recovery sea cucumber oil, the ethanol of each time alcohol precipitation collection is squeezed in the multifunctional alcohol recovery inspissator carried out the ethanol distillation recovery, the ethanol of recovery is after alcohol meter mensuration, and concentration is higher than 85% recycling precipitation with alcohol process; To remain thick liquid after ethanol reclaims collects; Again thick liquid was cooled off 5-10 hour under 0 ℃ to-10 ℃ environment, after the cooling solid mass is pulled out, solid mass is added boil 5-30 minute in the entry; Solid mass is 1 with the part by weight that adds water: 5-1: 20; Constantly stir in the boiling part, block oily solid is leached collection be the sea cucumber oil that contains the serial polyunsaturated fatty acid of ω-3, the key component of said sea cucumber oil is eicosapentaenoic acid EPA, DHA DHA and a small amount of salinity; Alcohol reclaims the pressure of inspissator at 0.05-0.09MPa; Vacuum is 0.03-0.08MPa, and recovered alcohol 200kg/h, steam consumption quantity are 1.9kg/h.
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| CN101991113B (en) * | 2010-11-30 | 2013-01-09 | 青岛佳日隆海洋食品有限公司 | Sea cucumber oil soft capsule and preparation method thereof |
| CN104522728B (en) * | 2014-11-27 | 2017-10-20 | 山东好当家海洋发展股份有限公司 | A kind of method that utilization sea cucumber blanching solution extracts phosphatide and oil of sea cucumber |
| CN107418708B (en) * | 2017-08-28 | 2020-11-17 | 山东好当家海洋发展股份有限公司 | Method for extracting sea cucumber oil from internal organs of sea cucumber |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1575663A (en) * | 2002-08-30 | 2005-02-09 | 大连轻工业学院 | Sea cucumber mucopolysaccharide-riched food and making method thereof |
| CN1985852A (en) * | 2006-12-12 | 2007-06-27 | 姜玉宝 | Process of extracting sea cucumber polyose and other active components from boiled sea cucumber juice |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1575663A (en) * | 2002-08-30 | 2005-02-09 | 大连轻工业学院 | Sea cucumber mucopolysaccharide-riched food and making method thereof |
| CN1985852A (en) * | 2006-12-12 | 2007-06-27 | 姜玉宝 | Process of extracting sea cucumber polyose and other active components from boiled sea cucumber juice |
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| Title |
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| 陈健等.糙海参酸性粘多糖的分离及特性研究.《食品与发酵工业》.2006,第32卷(第10期),123-126. * |
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