CN104522728B - A kind of method that utilization sea cucumber blanching solution extracts phosphatide and oil of sea cucumber - Google Patents
A kind of method that utilization sea cucumber blanching solution extracts phosphatide and oil of sea cucumber Download PDFInfo
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- CN104522728B CN104522728B CN201410695341.5A CN201410695341A CN104522728B CN 104522728 B CN104522728 B CN 104522728B CN 201410695341 A CN201410695341 A CN 201410695341A CN 104522728 B CN104522728 B CN 104522728B
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- 241000251511 Holothuroidea Species 0.000 title claims abstract description 86
- 206010033546 Pallor Diseases 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 26
- 239000000284 extract Substances 0.000 title claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 70
- 102000004190 Enzymes Human genes 0.000 claims abstract description 38
- 108090000790 Enzymes Proteins 0.000 claims abstract description 38
- 239000004365 Protease Substances 0.000 claims abstract description 34
- 239000012141 concentrate Substances 0.000 claims abstract description 32
- 108091005804 Peptidases Proteins 0.000 claims abstract description 28
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 28
- 239000000287 crude extract Substances 0.000 claims abstract description 15
- 238000006703 hydration reaction Methods 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 14
- 239000013049 sediment Substances 0.000 claims abstract description 14
- 238000005119 centrifugation Methods 0.000 claims abstract description 10
- 238000001816 cooling Methods 0.000 claims abstract description 5
- 238000010438 heat treatment Methods 0.000 claims abstract description 4
- 229940088598 enzyme Drugs 0.000 claims description 37
- 235000019419 proteases Nutrition 0.000 claims description 27
- 150000001875 compounds Chemical class 0.000 claims description 12
- 238000000926 separation method Methods 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 239000000796 flavoring agent Substances 0.000 claims description 9
- 235000019634 flavors Nutrition 0.000 claims description 9
- 229910052799 carbon Inorganic materials 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 108090000526 Papain Proteins 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 6
- 235000019834 papain Nutrition 0.000 claims description 6
- 229940055729 papain Drugs 0.000 claims description 6
- 239000003513 alkali Substances 0.000 claims description 2
- 238000001556 precipitation Methods 0.000 claims 1
- 230000036541 health Effects 0.000 abstract description 12
- 238000004519 manufacturing process Methods 0.000 abstract description 9
- 238000005516 engineering process Methods 0.000 abstract description 7
- 235000013305 food Nutrition 0.000 abstract description 6
- 238000003912 environmental pollution Methods 0.000 abstract description 5
- 230000000050 nutritive effect Effects 0.000 abstract description 2
- 150000003904 phospholipids Chemical class 0.000 abstract description 2
- 238000001291 vacuum drying Methods 0.000 abstract 1
- 235000016709 nutrition Nutrition 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 244000189799 Asimina triloba Species 0.000 description 2
- 235000006264 Asimina triloba Nutrition 0.000 description 2
- 235000009467 Carica papaya Nutrition 0.000 description 2
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 235000020673 eicosapentaenoic acid Nutrition 0.000 description 2
- 238000011031 large-scale manufacturing process Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 241000965254 Apostichopus japonicus Species 0.000 description 1
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 241000237852 Mollusca Species 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 101710093543 Probable non-specific lipid-transfer protein Proteins 0.000 description 1
- 206010039966 Senile dementia Diseases 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical group CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000002358 autolytic effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 230000003925 brain function Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 229960005135 eicosapentaenoic acid Drugs 0.000 description 1
- JAZBEHYOTPTENJ-UHFFFAOYSA-N eicosapentaenoic acid Natural products CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O JAZBEHYOTPTENJ-UHFFFAOYSA-N 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- JRZJOMJEPLMPRA-UHFFFAOYSA-N olefin Natural products CCCCCCCC=C JRZJOMJEPLMPRA-UHFFFAOYSA-N 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/08—Esters of oxyacids of phosphorus
- C07F9/09—Esters of phosphoric acids
- C07F9/10—Phosphatides, e.g. lecithin
- C07F9/103—Extraction or purification by physical or chemical treatment of natural phosphatides; Preparation of compositions containing phosphatides of unknown structure
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Seasonings (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention relates to a kind of method that utilization sea cucumber blanching solution extracts phosphatide and oil of sea cucumber, it is to choose fresh sea cucumber blanching solution for raw material, after being concentrated under reduced pressure, takes concentrate to dissolve by heating, protease hydrolyzed is used after cooling；Enzymolysis liquid goes out after enzyme using disk plate centrifuge centrifugation, collects enzymolysis light liquid；It will digest after light liquid hydration reaction, secondary centrifuging is carried out using butterfly centrifugal machine, and obtain sediment and light liquid；Light liquid is heated, vacuum drying, obtains oil of sea cucumber crude extract；Sediment is freeze-dried, and obtains sea cucumber phosphatide crude extract.The extracting method rational technology of the present invention, simple to operate, production cost is low, can reduce environmental pollution.It is high using the oil of sea cucumber that extracts of the present invention and sea cucumber phospholipid nutritive, health value, can extensively using with the field such as food, health care.
Description
Technical field
It is especially a kind of to extract phosphatide and sea using sea cucumber blanching solution the present invention relates to mollusc aquatic food product
Join the method for oil.
Background technology
Fresh sea cucumber is after fishing for, because its own has the enzyme of proteolysis such as autolytic enzyme, it is easy to change skin, self-dissolving.For
It is easy to preserve, fresh sea cucumber generally will go out through hot water blanching reprocesses storage after enzyme.And fresh sea cucumber is during hot water boils
Substantial amounts of blanching solution can be produced, through analysis and research, is removed in sea cucumber blanching solution and contains substantial amounts of glycoprotein（ZL 2011 1
0384722.8）, protein, saponin(e（The separation identification Shandong University of several selenka compounds in imitative stichopus japonicus processing-waste
Journal（Edition）7th phase in 2011）Outside Deng composition, also rich in the lipid component such as phosphatide and aliphatic acid.
At present, it yet there are no the report that phosphatide and oil of sea cucumber are extracted from sea cucumber blanching solution.
The content of the invention
It is an object of the invention to provide a kind of method that utilization sea cucumber blanching solution extracts phosphatide and oil of sea cucumber, the extracting method work
Rationally, simple to operate, production cost is low for skill；The two class active materials extracted are respectively provided with higher health value, while reducing sea
Environmental pollution after blanching solution discharge, with extensive social and economic benefit.
The technical solution adopted for the present invention to solve the technical problems is：One kind extracts phosphatide and sea using sea cucumber blanching solution
Join the method for oil, it is characterised in that：It is to choose sea cucumber blanching solution for raw material, and sea cucumber blanching solution is concentrated under reduced pressure, concentrate is obtained；
Concentrate is dissolved by heating, and is digested after cooling using protease, obtains enzymolysis liquid；Enzymolysis liquid go out after enzyme using disk plate centrifuge from
The heart is separated, and collects enzymolysis light liquid；Digest after light liquid hydration reaction, secondary centrifuging separation is carried out using butterfly centrifugal machine, must be precipitated
Thing and light liquid；Light liquid is heated, vacuum dried, obtains oil of sea cucumber crude extract；Sediment is freeze-dried, obtains sea cucumber phosphatide and slightly carries
Thing.
It is that sea cucumber blanching solution is concentrated under reduced pressure into solid content for 7~15% that described sea cucumber blanching solution, which is concentrated under reduced pressure,.
Described enzymolysis is the protease that concentrate is added to concentrate quality 1~8 ‰, controls 50~60 DEG C of temperature, enzyme
Solve 1~5h.
The described enzymolysis liquid enzyme that goes out is that enzymolysis liquid is heated into 90~100 DEG C, keeps 10~30min, carries out destroy the enzyme treatment.
Described enzymolysis light liquid hydration reaction is that enzymolysis light liquid is heated into 80~85 DEG C, and retort is pumped into 90 DEG C of water
In, 20~30min of stirring reaction.
A kind of method that utilization sea cucumber blanching solution extracts phosphatide and oil of sea cucumber, it is characterised in that：By following process steps：
（1）, raw material selection and processing
It is raw material to choose sea cucumber blanching solution, is concentrated under reduced pressure, and it is 7~15% to be concentrated into solid content, obtains concentrate；
（2）, enzymolysis
By step（1）In obtained concentrate dissolve by heating, cooling controls 50~60 DEG C of temperature, then will in concentrate plus
Enter the protease of concentrate quality 1~8 ‰, digest 1~5h, obtain enzymolysis liquid；
（3）, go out enzyme
By step（2）In obtained enzymolysis liquid be heated to 90~100 DEG C, keep 10~30min, carry out destroy the enzyme treatment, obtain
Go out the enzymolysis liquid of enzyme；
（4）, centrifugation
By step（3）In in the obtained enzymolysis liquid feeding disc centrifuge of the enzyme that goes out, centrifuge, collect enzymolysis light
Liquid；
（5）Hydration reaction
By step（4）In obtained enzymolysis light liquid be heated to 80~85 DEG C, be pumped into retort, stir, water with 90 DEG C of water
Change 20~30min of reaction；
（6）Secondary centrifuging
By step（5）In light liquid after obtained hydration reaction be transferred in butterfly centrifugal machine, secondary centrifuging separation is collected
Sediment and light liquid；
（7）Dry
By step（6）In obtained light liquid be heated to 110-115 DEG C, feeding vacuum desiccator is dried in vacuo, and obtains extra large
The oily crude extract of ginseng；
By step（6）In obtain sediment freeze-drying, obtain sea cucumber phosphatide crude extract.
Described protease is one kind or one in compound protease, flavor protease, papain, alkali protease
More than kind.
The present invention is the technique being combined by biological enzymolysis with physical separation, separated by concentration, enzymolysis, disc-type,
Aquation, disc-type two processing step such as separate, dried and sea cucumber phosphatide and oil of sea cucumber are produced from sea cucumber blanching solution.It is with existing
Leftover bits and pieces-sea cucumber blanching solution during Holothurian machining is waste sea cucumber phosphatide and oil of sea cucumber, and its production cost is low.By
Contain abundant protein, polypeptide, amino acid and sea cucumber flavor substance in sea cucumber blanching solution, not only taste is fresh for the oil of sea cucumber produced
U.S., and containing the serial polyunsaturated fatty acids of Ω -3, key component is eicosapentaenoic acid (abbreviation EPA), 22 carbon six
Olefin(e) acid (abbreviation DHA) and a small amount of salinity, its nutritional ingredient are more easy to be absorbed by the body；The sea cucumber phosphatide produced can adjust metabolism,
Strengthen physical efficiency；Improve brain function, strengthen memory；Adjust blood fat, reduction cholesterol, atherosclerosis；Improve liver
Function；Pre- preventing bone rarefaction and senile dementia etc..The extracting method of the present invention, rational technology is simple to operate, and production cost is low,
It is particularly suitable for large-scale production.There is the oil of sea cucumber and sea cucumber phosphatide extracted using the present invention higher nutrition and health care to be worth,
Can extensively using with the field such as food, health care, while the environmental pollution after the discharge of sea cucumber blanching solution can be also reduced, with extensive
Social and economic benefit.
Embodiment
With reference to embodiment, the present invention will be further described.
Embodiment 1
A kind of method that utilization sea cucumber blanching solution extracts phosphatide and oil of sea cucumber, by following process steps：
（1）, raw material selection and processing
It is raw material to choose sea cucumber blanching solution 4000L, and it is concentrated under reduced pressure through the equipment that is concentrated under reduced pressure, volume 800L is concentrated into,
Solid content is 10%, obtains concentrate；
（2）, enzymolysis
By step（1）In obtained concentrate be heated to 100 DEG C of holding 30min, fully dissolving, cooling controls temperature 55
DEG C, then the compound protease for concentrate quality 1 ‰ being added in concentrate, 1h is digested, enzymolysis liquid is obtained；Wherein, compound protease
The compound protease produced and sold for Ningxia jade of the He family Bioisystech Co., Ltd；
（3）, go out enzyme
By step（2）In obtained enzymolysis liquid be heated to 100 DEG C, keep 10min, carry out destroy the enzyme treatment, the enzyme for the enzyme that must go out
Solve liquid；
（4）, centrifugation
By step（3）In the obtained enzymolysis liquid of the enzyme that goes out be cooled to 25 DEG C, be then fed into disc centrifuge, control turns
Fast 6000rpm, is centrifuged, and collects enzymolysis light liquid；
（5）Aquation
By step（4）In obtained enzymolysis light liquid be heated to 83 DEG C, be pumped into retort, stir with 90 DEG C of water, aquation is anti-
Answer 25min；
（6）Secondary centrifuging
By step（5）In enzymolysis light liquid after obtained hydration reaction be transferred in butterfly centrifugal machine, control rotating speed
6000rpm, secondary centrifuging separation, collects sediment and light liquid；
（7）Dry
By step（6）In obtained light liquid be heated to 112 DEG C, feeding vacuum desiccator is dried in vacuo, and obtains oil of sea cucumber
Crude extract；
By step（6）In obtain sediment freeze-drying, obtain sea cucumber phosphatide crude extract.
The utilization sea cucumber blanching solution that the present embodiment is provided extracts the method for phosphatide and oil of sea cucumber, its rational technology, operation letter
Single, production cost is low, is particularly suitable for large-scale production.The oil of sea cucumber and sea cucumber phosphatide extracted using the present invention has higher battalion
Support and health value, can extensively using with the field such as food, health care.
Embodiment 2
A kind of method that utilization sea cucumber blanching solution extracts phosphatide and oil of sea cucumber, by following process steps：
（1）, raw material selection and processing
It is raw material to choose sea cucumber blanching solution 4000L, is sent to be concentrated under reduced pressure in equipment and is concentrated under reduced pressure, and is concentrated into body
Product 1000L, solid content is 5% or so, obtains concentrate；
（2）, enzymolysis
By step（1）In obtained concentrate heat 120 DEG C of holding 10min, fully dissolving, chilling temperature to 60 DEG C, then
The compound protease of concentrate quality 2 ‰ will be added in concentrate, digest 1h, then add the local flavor egg of concentrate quality 2 ‰
White enzyme, hydrolyzes 2.5h, obtains enzymolysis liquid；Wherein, compound protease is commercially available Novi's letter compound protease, and flavor protease is
The flavor protease that the enzyme-linked biological study in Shanghai is produced and sold；
（3）, go out enzyme
By step（2）In obtained enzymolysis liquid be heated to 95 DEG C, keep 20min, carry out destroy the enzyme treatment, the enzymolysis for the enzyme that must go out
Liquid；
（4）, centrifugation
By step（3）In obtain the enzyme that goes out enzymolysis liquid feeding disc centrifuge in, control rotating speed 6000rpm, centrifugation
Separation, collects enzymolysis light liquid；
（5）Hydration reaction
By step（4）In obtained enzymolysis light liquid be heated to 80 DEG C, be pumped into retort, stir with 90 DEG C of water, aquation is anti-
Answer 30min；
（6）Secondary centrifuging
By step（5）In enzymolysis light liquid after obtained hydration reaction be transferred in butterfly centrifugal machine, control rotating speed
6000rpm, secondary centrifuging separation, collects sediment and light liquid；
（7）Dry
By step（6）In obtained light liquid be heated to 115 DEG C, feeding vacuum desiccator is dried in vacuo, and obtains oil of sea cucumber
Crude extract；
By step（6）In obtain sediment freeze-drying, obtain sea cucumber phosphatide crude extract.
The utilization sea cucumber blanching solution that the present embodiment is provided extracts the method for phosphatide and oil of sea cucumber, its rational technology, operation letter
Single, production cost is low.There is the oil of sea cucumber and sea cucumber phosphatide extracted using the present invention higher nutrition and health care to be worth.
Embodiment 3
A kind of method that utilization sea cucumber blanching solution extracts phosphatide and oil of sea cucumber, by following process steps：
（1）, raw material selection and processing
It is raw material to choose sea cucumber blanching solution 4000L, is sent to be concentrated under reduced pressure in equipment and is concentrated under reduced pressure, and is concentrated into body
Product 500L, solid content is 15% or so, obtains concentrate；
（2）, enzymolysis
By step（1）In obtained concentrate heat 90 DEG C of holding 30min, fully dissolving, chilling temperature, then will to 50 DEG C
The papain of concentrate quality 3 ‰ is added in concentrate, 2h is digested, the basic protein of concentrate quality 2 ‰ is then added
Enzyme, hydrolyzes 3h, obtains enzymolysis liquid；Wherein, papain is the papain that Shanghai Shi Feng bio tech ltd is sold,
Neutral proteinase is the neutral proteinase that the producing and selling of hill Co., Ltd are raised by Sichuan Xinhua；
（3）, go out enzyme
By step（2）In obtained enzymolysis liquid be heated to 90 DEG C, keep 30min, carry out destroy the enzyme treatment, the enzymolysis for the enzyme that must go out
Liquid；
（4）, centrifugation
By step（3）In obtain the enzyme that goes out enzymolysis liquid feeding disc centrifuge in, control rotating speed 4000rpm, centrifugation
Separation, collects enzymolysis light liquid；
（5）Hydration reaction
By step（4）In obtained enzymolysis light liquid be heated to 85 DEG C, be pumped into retort, stir with 90 DEG C of water, aquation is anti-
Answer 20min；
（6）Secondary centrifuging
By step（5）In enzymolysis light liquid after obtained hydration reaction be transferred in butterfly centrifugal machine, control rotating speed
4000rpm, secondary centrifuging separation, collects sediment and light liquid；
（7）Dry
By step（6）In obtained light liquid be heated to 110 DEG C, feeding vacuum desiccator is dried in vacuo, and obtains oil of sea cucumber
Crude extract；
By step（6）In obtain sediment freeze-drying, obtain sea cucumber phosphatide crude extract.
The utilization sea cucumber blanching solution that the present embodiment is provided extracts the method for phosphatide and oil of sea cucumber, its rational technology, operation letter
Single, production cost is low, reduces environmental pollution.Oil of sea cucumber and sea cucumber phospholipid nutritive, the health value extracted using the present invention is high, can
Extensively using with the field such as food, health care.
Embodiment 4
A kind of method that utilization sea cucumber blanching solution extracts phosphatide and oil of sea cucumber, by following process steps：
（1）, raw material selection and processing
It is raw material to choose sea cucumber blanching solution 4000L, and it is concentrated under reduced pressure through the equipment that is concentrated under reduced pressure, volume 700L is concentrated into,
Solid content is 12%, obtains concentrate；
（2）, enzymolysis
By step（1）In obtained concentrate heat 100 DEG C of holding 30min, fully dissolving, chilling temperature to 55 DEG C, then
The compound protease of concentrate quality 2 ‰ will be added in concentrate, digest 1h, then add the pawpaw egg of concentrate quality 3 ‰
White enzyme, hydrolyzes 2h, then adds the flavor protease of concentrate quality 3 ‰, hydrolyzes 1h, obtains enzymolysis liquid；Wherein, compound protease
It is commercially available Novi's letter compound protease, papain is the pawpaw of Pangbo Bioengineering Co Ltd, Nanning's production and sales
Protease；Flavor protease is Novi's letter flavor protease that city sells；
（3）, go out enzyme
By step（2）In obtained enzymolysis liquid be heated to 100 DEG C, keep 30min, carry out destroy the enzyme treatment, the enzyme for the enzyme that must go out
Solve liquid；
（4）, centrifugation
By step（3）In obtain the enzyme that goes out enzymolysis liquid feeding disc centrifuge in, control rotating speed 5000rpm, centrifugation
Separation, collects enzymolysis light liquid；
（5）Hydration reaction
By step（4）In obtained enzymolysis light liquid be heated to 85 DEG C, be pumped into retort, stir with 90 DEG C of water, aquation is anti-
Answer 30min；
（6）Secondary centrifuging
By step（5）In enzymolysis light liquid after obtained hydration reaction be transferred in butterfly centrifugal machine, control rotating speed
5000rpm, secondary centrifuging separation, collects sediment and light liquid；
（7）Dry
By step（6）In obtained light liquid be heated to 115 DEG C, feeding vacuum desiccator is dried in vacuo, and obtains oil of sea cucumber
Crude extract；
By step（6）In obtain sediment freeze-drying, obtain sea cucumber phosphatide crude extract.
The utilization sea cucumber blanching solution that the present embodiment is provided extracts the method for phosphatide and oil of sea cucumber, its rational technology, operation letter
Single, production cost is low, the environmental pollution after the discharge of sea cucumber blanching solution can be reduced, with extensive social and economic benefit.Using
There is the oil of sea cucumber and sea cucumber phosphatide that the present invention is extracted higher nutrition and health care to be worth, can be extensively using with food, health care etc.
Field.
Claims (1)
1. a kind of method that utilization sea cucumber blanching solution extracts phosphatide and oil of sea cucumber, it is characterised in that：By following process steps：
（1）, raw material selection and processing
It is raw material to choose sea cucumber blanching solution, is concentrated under reduced pressure, and it is 7~15% to be concentrated into solid content, obtains concentrate；
（2）, enzymolysis
By step（1）In obtained concentrate dissolve by heating, cooling controls 50~60 DEG C of temperature, then dense by being added in concentrate
The protease of contracting liquid quality 1~8 ‰, digests 1~5h, obtains enzymolysis liquid；Wherein, described protease is compound protease, local flavor
One or more of protease, papain, alkali protease；
（3）, go out enzyme
By step（2）In obtained enzymolysis liquid be heated to 90~100 DEG C, keep 10~30min, carry out destroy the enzyme treatment, must go out enzyme
Enzymolysis liquid；
（4）, centrifugation
By step（3）In obtain the enzyme that goes out enzymolysis liquid feeding disc centrifuge in, centrifuge, collect enzymolysis light liquid；
（5）, hydration reaction
By step（4）In obtained enzymolysis light liquid be heated to 80~85 DEG C, be pumped into retort, stir with 90 DEG C of water, aquation is anti-
Answer 20~30min；
（6）, secondary centrifuging
By step（5）In light liquid after obtained hydration reaction be transferred in butterfly centrifugal machine, precipitation is collected in secondary centrifuging separation
Thing and light liquid；
（7）, dry
By step（6）In obtained light liquid be heated to 110~115 DEG C, feeding vacuum desiccator is dried in vacuo, and obtains oil of sea cucumber
Crude extract；
By step（6）In obtain sediment freeze-drying, obtain sea cucumber phosphatide crude extract.
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