CN105331439A - Method for extracting soybean blend oil and compound protein by using aqueous enzymatic method - Google Patents
Method for extracting soybean blend oil and compound protein by using aqueous enzymatic method Download PDFInfo
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Abstract
The invention relates to a method for extracting soybean blend oil and compound protein by using an aqueous enzymatic method, which belongs to the technical field of vegetable oil and fat and protein processing. The method comprises the following steps of (1) proportionally mixing soybeans and peanuts, then peeling, crushing, and then extruding and expanding, so as to obtain an expanded product; (2) adding water to an enzymolysis reaction kettle, heating with steam, then adding the expanded product, so as to obtain a mixed solution, regulating a pH value of the mixed solution, then adding alkaline protease for enzymolysis, and centrifuging after the enzymolysis, so as to obtain emulsified oil, hydrolysate and residues; (3) performing ethanol demulsification on the emulsified oil, and then centrifuging to obtain free oil and a solid phase; (4) adding the solid phase to the hydrolysate for alkali extraction and acid precipitation, and then centrifuging to obtain a liquid phase and sediments; (5) performing steam demulsification on the liquid phase, then centrifuging to obtain free oil, merging the free oil, so as to obtain the soybean blend oil, and dying the sediments, so as to obtain the compound protein. The method has the advantages of simple process and low cost, the prepared soybean blend oil has strong oil fragrance, the prepared compound protein has good functionality, and the method is favorable for being applied in a food industry.
Description
Technical field
The invention belongs to Vegetable oil lipoprotein and Protein processing technical field, relate generally to the method for aqueous enzymatic extraction soybean mixed oil and conjugated protein.
Background technology
The edible oil of people's current consumption mainly contains: sunflower seed oil, peanut oil, soybean oil, vegetable seed wet goods, general is all the grease obtained by single Raw material processing, often eat single edible oil and can cause the insufficiency of intake of certain or several lipid acid or excessive, thus the metabolic balance in human body is changed, cause various disease.Scientific and reasonablely balancedly take in diverse lipid acid, to the good function maintaining tissue, keep lasting health to be absolutely necessary.
Traditional mixed oil method of preparing is all mix after first being proposed by single grease, carrying out allotment to compound lard again makes its lipid acid reach balance, this method technique is loaded down with trivial details, during suitability for industrialized production, production line is too much, production cost is high, and all will carry out index determining to often kind of grease, workload is large at every turn.
To be that of growing up at 20 century 70s is emerging carry oil tech with aqueous enzymatic extraction vegetables oil, as a kind of emerging Vegetable oil lipoprotein extractive technique, be on the basis of Mechanical Crushing, enzymolysis is carried out to oil plant tissue and the complex body such as lipoprotein, lipopolysaccharides, thus make grease free out.Adopt in aqueous enzymatic extraction Vegetable oil lipoprotein process, protein in oil plant can be obtained simultaneously.Compared with traditional technology, aqueous enzymatic method is carried oil tech and is had that energy consumption is low, environmental protection, safety and sanitation, reaction conditions is gentle, and degraded product can not react with extract, grease no solvent residue and the advantages such as quality better, applied in the multiple oil plants such as soybean, peanut, linseed oil and tea seed.Current aqueous enzymatic method is carried oily great majority and is confined to laboratory study, because aqueous enzymatic method puies forward in oily process problems such as there is zymin cost is high, long reaction time, breakdown of emulsion difficulty, seldom can put in production practice, little to the commercial application research of aqueous enzymatic method.
Based on the practical situation of suitability for industrialized production, the inventive method selects the peanut of the soybean of the low oil of high protein and the low albumen of height oil to be raw materials for production, utilize the complementation of nutritive ingredient, after the two is mixed in proportion, the mixed oil of high-quality and functional conjugated protein is prepared through aqueous enzymatic extraction, mainly comprising expanded, enzymolysis, breakdown of emulsion, the technical process such as centrifugal, dry, is industrialization continuous seepage soybean mixed oil and conjugated protein based theoretical.
Summary of the invention
Technical problem to be solved by this invention overcomes the deficiencies in the prior art, provides a kind of method of aqueous enzymatic extraction soybean mixed oil and conjugated protein, reach Simplified flowsheet, reduce costs, improve the object of product quality.
Technical problem to be solved by this invention is achieved through the following technical solutions:
The method of aqueous enzymatic extraction soybean mixed oil and conjugated protein, the method comprises the following steps: soybean and peanut are mixed in proportion by (1), described soybean and Quality of Peanuts are than being 1-3:1, carry out after mixing peeling, pulverization process, then regulate moisture to carry out extruding puffing process and obtain expanded product, described material water ratio is 18%, and jacket temperature is 95 DEG C, screw speed is 110r/min, and nib aperture is 20mm; (2) in enzyme digestion reaction still, water is added, pass into steam to heat, described steam heating temperature is 50-60 DEG C, in the water after heating, add expanded product carry out being mixed to get mixed solution, the NaOH solution of 2mol/L is adopted to regulate the initial pH of mixed solution to be 8.5, then in mixed solution, add Alcalase Sumizyme MP carry out enzymolysis, described enzyme concentration is the 2.5-5 ‰ of mixed solution quality, solid-liquid ratio is 1:6-8, enzymolysis time is 40-60min, adopts triphase horizontal whizzer to carry out centrifugation and obtain oil in water emulsion, hydrolyzed solution and residue after enzymolysis; (3) in oil in water emulsion, add ethanol and carry out breakdown of emulsion, described ethanol and oil in water emulsion volume ratio are 1-5:1, the ethanol breakdown of emulsion time is 20-40min, adopt two-phase horizontal centrifuge to carry out centrifugation after ethanol breakdown of emulsion and obtain oil phase and solid phase, oil phase is carried out underpressure distillation and obtains free oil, reclaim ethanol simultaneously; (4) solid phase is added in hydrolyzed solution and carry out alkali extraction and acid precipitation after mixing, then adopt two-phase horizontal centrifuge to carry out centrifugation and obtain liquid phase and precipitation; (5) pass into hot steam to liquid phase and carry out breakdown of emulsion, described vapor temperature is 110-130 DEG C, and the steam breakdown of emulsion time is 6-10s, adopts disc centrifuge to carry out centrifugation and obtain free oil after steam breakdown of emulsion, merge free oil and be soybean mixed oil, precipitation is carried out spraying dry and obtains conjugated protein.
Described preferred soybean and Quality of Peanuts are than being 2:1.
Described preferred steam heating temperature is 55 DEG C.
Described enzymolysis preferred parameter is: enzyme concentration is 3.5 ‰ of mixed solution quality, solid-liquid ratio 1:7, enzymolysis time 50min.
Described ethanol breakdown of emulsion preferred parameter is: ethanol and oil in water emulsion volume ratio 3:1, ethanol breakdown of emulsion time 30min.
Described steam breakdown of emulsion preferred parameter is: vapor temperature 120 DEG C, steam breakdown of emulsion time 8s.
Present method for raw material with soybean and peanut, adopts extruding puffing to carry out pre-treatment, can effectively destroy material cell walls, make the suitable sex change of protein simultaneously, expose enzymolysis site, be beneficial to enzymolysis.Then in enzyme digestion reaction still, carry out enzymolysis processing to expanded product, the initial pH of enzymolysis is proteolytic enzyme peak enzymolysis-ability pH8.5, along with the carrying out of reaction, pH constantly declines, and at the end of enzymolysis, the pH of enzymolysis solution reaches neutral, without the need to acid neutralization, can not salt be produced, eliminate desalinating process.After enzymolysis, ethanol break milk separation free oil is carried out to oil in water emulsion, obtain Solid phase protein simultaneously, be mixed in hydrolyzed solution after carrying out alkali extraction and acid precipitation and carry out centrifugation, steam breakdown of emulsion is carried out to liquid phase and obtains free oil, merge free oil and be soybean mixed oil, precipitation is carried out spraying dry and obtains conjugated protein.The method has the advantages that technique is simple, enzyme dosage is few, cost is low.Obtained soybean mixed oil comprehensive nutrition, balanced fatty acid proportion, the fragrance of peanut oil compensate for beany flavor, has strong salted cake fried in sesame oil taste; More comprehensively, soybean protein compensate for the shortcoming of the functional difference of Semen arachidis hypogaeae protein to obtained conjugated protein amino acid composition, is beneficial to application in the food industry.
Embodiment
The method of aqueous enzymatic extraction soybean mixed oil and conjugated protein, the method comprises the following steps: soybean and peanut are mixed in proportion by (1), described soybean and Quality of Peanuts are than being 1-3:1, carry out after mixing peeling, pulverization process, then regulate moisture to carry out extruding puffing process and obtain expanded product, described material water ratio is 18%, and jacket temperature is 95 DEG C, screw speed is 110r/min, and nib aperture is 20mm; (2) in enzyme digestion reaction still, water is added, pass into steam to heat, described steam heating temperature is 50-60 DEG C, in the water after heating, add expanded product carry out being mixed to get mixed solution, the NaOH solution of 2mol/L is adopted to regulate the initial pH of mixed solution to be 8.5, then in mixed solution, add Alcalase Sumizyme MP carry out enzymolysis, described enzyme concentration is the 2.5-5 ‰ of mixed solution quality, solid-liquid ratio is 1:6-8, enzymolysis time is 40-60min, adopts triphase horizontal whizzer to carry out centrifugation and obtain oil in water emulsion, hydrolyzed solution and residue after enzymolysis; (3) in oil in water emulsion, add ethanol and carry out breakdown of emulsion, described ethanol and oil in water emulsion volume ratio are 1-5:1, the ethanol breakdown of emulsion time is 20-40min, adopt two-phase horizontal centrifuge to carry out centrifugation after ethanol breakdown of emulsion and obtain oil phase and solid phase, oil phase is carried out underpressure distillation and obtains free oil, reclaim ethanol simultaneously; (4) solid phase is added in hydrolyzed solution and carry out alkali extraction and acid precipitation after mixing, then adopt two-phase horizontal centrifuge to carry out centrifugation and obtain liquid phase and precipitation; (5) pass into hot steam to liquid phase and carry out breakdown of emulsion, described vapor temperature is 110-130 DEG C, and the steam breakdown of emulsion time is 6-10s, adopts disc centrifuge to carry out centrifugation and obtain free oil after steam breakdown of emulsion, merge free oil and be soybean mixed oil, precipitation is carried out spraying dry and obtains conjugated protein.
Described preferred soybean and Quality of Peanuts are than being 2:1.
Described preferred steam heating temperature is 55 DEG C.
Described enzymolysis preferred parameter is: enzyme concentration is 3.5 ‰ of mixed solution quality, solid-liquid ratio 1:7, enzymolysis time 50min.
Described ethanol breakdown of emulsion preferred parameter is: ethanol and oil in water emulsion volume ratio 3:1, ethanol breakdown of emulsion time 30min.
Described steam breakdown of emulsion preferred parameter is: vapor temperature 120 DEG C, steam breakdown of emulsion time 8s.
Embodiment 1:
Carry out after soybean is mixed in the ratio of 2:1 with peanut peeling, pulverization process, then regulate moisture, material water ratio be 18%, jacket temperature is 95 DEG C, screw speed is 110r/min, nib aperture is carried out extruding puffing process under being the condition of 20mm and obtained expanded product, water is added in enzyme digestion reaction still, the steam passing into 55 DEG C heats, in the water after heating, add expanded product carry out being mixed to get mixed solution, the NaOH solution of 2mol/L is adopted to regulate the initial pH of mixed solution to be 8.5, the Alcalase Sumizyme MP adding 3.5 ‰ under solid-liquid ratio is 1:7 condition in mixed solution carries out enzymolysis 50min, adopts triphase horizontal whizzer to carry out centrifugation and obtain oil in water emulsion, hydrolyzed solution and residue after enzymolysis, under ethanol and oil in water emulsion volume ratio are the condition of 3:1, in oil in water emulsion, add ethanol carry out breakdown of emulsion 30min, adopt two-phase horizontal centrifuge to carry out centrifugation after ethanol breakdown of emulsion and obtain oil phase and solid phase, oil phase is carried out underpressure distillation and obtains free oil, reclaim ethanol simultaneously, solid phase is added in hydrolyzed solution and carry out alkali extraction and acid precipitation after mixing, then adopt two-phase horizontal centrifuge to carry out centrifugation and obtain liquid phase and precipitation, under vapor temperature is 120 DEG C of conditions, passes into hot steam to liquid phase carries out breakdown of emulsion 8s, adopt disc centrifuge to carry out centrifugation after steam breakdown of emulsion and obtain free oil, merge free oil and be soybean mixed oil, precipitation is carried out spraying dry and obtains conjugated protein.The method technique is simple, enzyme dosage is few, greatly reduces production cost, is applicable to industrialization continuous seepage, and can also obtain conjugated protein while obtained soybean mixed oil, grease extraction efficiency is 84.63%, and protein extracting ratio is 88.27%.This soybean mixed oil comprehensive nutrition, balanced fatty acid proportion, the fragrance of peanut oil compensate for beany flavor, has strong salted cake fried in sesame oil taste; More comprehensively, soybean protein compensate for the shortcoming of the functional difference of Semen arachidis hypogaeae protein to this conjugated protein amino acid composition, is beneficial to application in the food industry.
Embodiment 2:
Carry out after soybean is mixed in the ratio of 1:1 with peanut peeling, pulverization process, then regulate moisture, material water ratio be 18%, jacket temperature is 95 DEG C, screw speed is 110r/min, nib aperture is carried out extruding puffing process under being the condition of 20mm and obtained expanded product, water is added in enzyme digestion reaction still, the steam passing into 50 DEG C heats, in the water after heating, add expanded product carry out being mixed to get mixed solution, the NaOH solution of 2mol/L is adopted to regulate the initial pH of mixed solution to be 8.5, the Alcalase Sumizyme MP adding 4 ‰ under solid-liquid ratio is 1:8 condition in mixed solution carries out enzymolysis 40min, adopts triphase horizontal whizzer to carry out centrifugation and obtain oil in water emulsion, hydrolyzed solution and residue after enzymolysis, under ethanol and oil in water emulsion volume ratio are the condition of 2:1, in oil in water emulsion, add ethanol carry out breakdown of emulsion 40min, adopt two-phase horizontal centrifuge to carry out centrifugation after ethanol breakdown of emulsion and obtain oil phase and solid phase, oil phase is carried out underpressure distillation and obtains free oil, reclaim ethanol simultaneously, solid phase is added in hydrolyzed solution and carry out alkali extraction and acid precipitation after mixing, then adopt two-phase horizontal centrifuge to carry out centrifugation and obtain liquid phase and precipitation, under vapor temperature is 125 DEG C of conditions, passes into hot steam to liquid phase carries out breakdown of emulsion 6s, adopt disc centrifuge to carry out centrifugation after steam breakdown of emulsion and obtain free oil, merge free oil and be soybean mixed oil, precipitation is carried out spraying dry and obtains conjugated protein.The method technique is simple, enzyme dosage is few, greatly reduces production cost, is applicable to industrialization continuous seepage, and can also obtain conjugated protein while obtained soybean mixed oil, grease extraction efficiency is 83.12%, and protein extracting ratio is 88.69%.This soybean mixed oil comprehensive nutrition, balanced fatty acid proportion, the fragrance of peanut oil compensate for beany flavor, has strong salted cake fried in sesame oil taste; More comprehensively, soybean protein compensate for the shortcoming of the functional difference of Semen arachidis hypogaeae protein to this conjugated protein amino acid composition, is beneficial to application in the food industry.
Embodiment 3:
Carry out after soybean is mixed in the ratio of 3:1 with peanut peeling, pulverization process, then regulate moisture, material water ratio be 18%, jacket temperature is 95 DEG C, screw speed is 110r/min, nib aperture is carried out extruding puffing process under being the condition of 20mm and obtained expanded product, water is added in enzyme digestion reaction still, the steam passing into 60 DEG C heats, in the water after heating, add expanded product carry out being mixed to get mixed solution, the NaOH solution of 2mol/L is adopted to regulate the initial pH of mixed solution to be 8.5, the Alcalase Sumizyme MP adding 3 ‰ under solid-liquid ratio is 1:6 condition in mixed solution carries out enzymolysis 60min, adopts triphase horizontal whizzer to carry out centrifugation and obtain oil in water emulsion, hydrolyzed solution and residue after enzymolysis, under ethanol and oil in water emulsion volume ratio are the condition of 4:1, in oil in water emulsion, add ethanol carry out breakdown of emulsion 20min, adopt two-phase horizontal centrifuge to carry out centrifugation after ethanol breakdown of emulsion and obtain oil phase and solid phase, oil phase is carried out underpressure distillation and obtains free oil, reclaim ethanol simultaneously, solid phase is added in hydrolyzed solution and carry out alkali extraction and acid precipitation after mixing, then adopt two-phase horizontal centrifuge to carry out centrifugation and obtain liquid phase and precipitation, under vapor temperature is 115 DEG C of conditions, passes into hot steam to liquid phase carries out breakdown of emulsion 10s, adopt disc centrifuge to carry out centrifugation after steam breakdown of emulsion and obtain free oil, merge free oil and be soybean mixed oil, precipitation is carried out spraying dry and obtains conjugated protein.The method technique is simple, enzyme dosage is few, greatly reduces production cost, is applicable to industrialization continuous seepage, and can also obtain conjugated protein while obtained soybean mixed oil, grease extraction efficiency is 83.86%, and protein extracting ratio is 87.73%.This soybean mixed oil comprehensive nutrition, balanced fatty acid proportion, the fragrance of peanut oil compensate for beany flavor, has strong salted cake fried in sesame oil taste; More comprehensively, soybean protein compensate for the shortcoming of the functional difference of Semen arachidis hypogaeae protein to this conjugated protein amino acid composition, is beneficial to application in the food industry.
Claims (6)
1. the method for aqueous enzymatic extraction soybean mixed oil and conjugated protein, it is characterized in that the method comprises the following steps: soybean and peanut are mixed in proportion by (1), described soybean and Quality of Peanuts are than being 1-3:1, carry out after mixing peeling, pulverization process, then regulate moisture to carry out extruding puffing process and obtain expanded product, described material water ratio is 18%, and jacket temperature is 95 DEG C, screw speed is 110r/min, and nib aperture is 20mm; (2) in enzyme digestion reaction still, water is added, pass into steam to heat, described steam heating temperature is 50-60 DEG C, in the water after heating, add expanded product carry out being mixed to get mixed solution, the NaOH solution of 2mol/L is adopted to regulate the initial pH of mixed solution to be 8.5, then in mixed solution, add Alcalase Sumizyme MP carry out enzymolysis, described enzyme concentration is the 2.5-5 ‰ of mixed solution quality, solid-liquid ratio is 1:6-8, enzymolysis time is 40-60min, adopts triphase horizontal whizzer to carry out centrifugation and obtain oil in water emulsion, hydrolyzed solution and residue after enzymolysis; (3) in oil in water emulsion, add ethanol and carry out breakdown of emulsion, described ethanol and oil in water emulsion volume ratio are 1-5:1, the ethanol breakdown of emulsion time is 20-40min, adopt two-phase horizontal centrifuge to carry out centrifugation after ethanol breakdown of emulsion and obtain oil phase and solid phase, oil phase is carried out underpressure distillation and obtains free oil, reclaim ethanol simultaneously; (4) solid phase is added in hydrolyzed solution and carry out alkali extraction and acid precipitation after mixing, then adopt two-phase horizontal centrifuge to carry out centrifugation and obtain liquid phase and precipitation; (5) pass into hot steam to liquid phase and carry out breakdown of emulsion, described vapor temperature is 110-130 DEG C, and the steam breakdown of emulsion time is 6-10s, adopts disc centrifuge to carry out centrifugation and obtain free oil after steam breakdown of emulsion, merge free oil and be soybean mixed oil, precipitation is carried out spraying dry and obtains conjugated protein.
2. the method for aqueous enzymatic extraction soybean mixed oil according to claim 1 and conjugated protein, is characterized in that described preferred soybean and Quality of Peanuts are than being 2:1.
3. the method for aqueous enzymatic extraction soybean mixed oil according to claim 1 and conjugated protein, is characterized in that described preferred steam heating temperature is 55 DEG C.
4. the method for aqueous enzymatic extraction soybean mixed oil according to claim 1 and conjugated protein, is characterized in that described enzymolysis preferred parameter is: enzyme concentration is 3.5 ‰ of mixed solution quality, solid-liquid ratio 1:7, enzymolysis time 50min.
5. the method for aqueous enzymatic extraction soybean mixed oil according to claim 1 and conjugated protein, is characterized in that described ethanol breakdown of emulsion preferred parameter is: ethanol and oil in water emulsion volume ratio 3:1, ethanol breakdown of emulsion time 30min.
6. the method for aqueous enzymatic extraction soybean mixed oil according to claim 1 and conjugated protein, is characterized in that described steam breakdown of emulsion preferred parameter is: vapor temperature 120 DEG C, steam breakdown of emulsion time 8s.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105925365A (en) * | 2016-05-17 | 2016-09-07 | 东北农业大学 | Method for extracting soybean oil and non-bitter soybean peptide through aqueous enzymatic method |
| CN105950275A (en) * | 2016-05-17 | 2016-09-21 | 东北农业大学 | Aqueous enzymatic method for preparing blend sesame oil and composite protein peptide |
| CN107474941A (en) * | 2017-09-26 | 2017-12-15 | 绥化学院 | The method that aqueous enzymatic method synchronously extracts sesame oil and sesame polypeptide powder |
| CN108441536A (en) * | 2018-04-04 | 2018-08-24 | 陕西科技大学 | A method of improving rapeseed protein emulsifying activity |
| CN108913328A (en) * | 2018-08-04 | 2018-11-30 | 望江县振兴植物油厂（普通合伙） | A kind of preparation process of nutrient blend oil |
| CN109181844A (en) * | 2018-09-14 | 2019-01-11 | 许昌鑫瑞德化工科技有限公司 | A kind of modified vegetable and animals oils extracting method |
| CN109363021A (en) * | 2018-10-16 | 2019-02-22 | 宾川县华侨庄园农业科技开发有限公司 | A kind of processing method of orange blossom |
| CN115812841A (en) * | 2022-12-14 | 2023-03-21 | 中国农业科学院农产品加工研究所 | Method for efficiently and synchronously separating oil and protein in peanut oil aqueous enzymatic preparation |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105925365A (en) * | 2016-05-17 | 2016-09-07 | 东北农业大学 | Method for extracting soybean oil and non-bitter soybean peptide through aqueous enzymatic method |
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| CN107474941A (en) * | 2017-09-26 | 2017-12-15 | 绥化学院 | The method that aqueous enzymatic method synchronously extracts sesame oil and sesame polypeptide powder |
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