CN101671400A - Method of preparing soluble soybean polysaccharide - Google Patents
Method of preparing soluble soybean polysaccharide Download PDFInfo
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- CN101671400A CN101671400A CN200910186212A CN200910186212A CN101671400A CN 101671400 A CN101671400 A CN 101671400A CN 200910186212 A CN200910186212 A CN 200910186212A CN 200910186212 A CN200910186212 A CN 200910186212A CN 101671400 A CN101671400 A CN 101671400A
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- soybean polysaccharide
- soluble soybean
- soybean
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Abstract
The invention relates to a method of preparing soluble soybean polysaccharide which is characterized by comprising the following steps of homogenizing bean dregs formed after the bean product processing, hydrolyzing the bean dregs by enzyme, washing the bean dregs, extracting extract from bean dreg fiber under weak-acid high-temperature condition, centrifuging the extract to obtain supernate, precipitating the supernate for desalinating, filtering the supernate by a film for purifying, drying the concentrated solution of the soluble soybean polysaccharide through spraying, detecting the soluble soybean polysaccharide and packaging the soluble soybean polysaccharide. The invention has the advantages of greatly improving the soluble soybean polysaccharide production efficiency, lowering thesoluble soybean polysaccharide production cost, reducing the pollution of the environment, greatly improving the soluble soybean polysaccharide extracting rate under the weak-acid high-temperature condition, ensuring that the flavor of the soluble soybean polysaccharide is acceptable to consumers through decomposing unpleasant odor in the raw materials by steaming the raw materials at high temperature, precipitating and desalinating the extracted supernate with the precipitating and desalinating technology, separating the soluble soybean polysaccharide with the centrifugal separation method, and drying the soluble soybean polysaccharide into powder through spraying. In addition, the invention has simple soluble soybean polysaccharide process and is easy to realize the industrialization.
Description
Technical field
The present invention relates to a kind of preparation method of soybean polysaccharide, relate in particular to a kind of preparation method of water soluble soybean polysaccharide.
Background technology
The production method of water soluble soybean polysaccharide has multiple, there is following shortcoming in existing production method: 1, acid is carried, to put forward technology be the traditional production technique of producing soluble soybean polysaccharide at present to alkali, but this technology yield is low, and product quality is uneven, and having relatively high expectations to equipment; 2, the purity of the soluble soybean polysaccharide that obtains of sequestrant extract at low temperature technology is low, and the sequestrant usage quantity is many, remove difficulty, and extraction time is long, and cost is higher; 3, the water soluble soybean polysaccharide product purity is not high at present, and cost is higher, costs an arm and a leg, and can not be widely used.4, the poor quality of existing water soluble soybean polysaccharide product has unpleasant odor.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of water soluble soybean polysaccharide, this method is a raw material with bean dregs (bean product process residues), adopts homogeneous, enzymolysis, washing, weak acid high temperature extraction, centrifugal, precipitation desalination, membrane separation purification, concentrates, modern high technology such as spraying drying produces the water soluble soybean polysaccharide product.
The present invention is achieved like this, and it is characterized in that the preparation method is:
1, the bean dregs homogeneous that forms of bean product processing back: (1) transfers liquid: bean dregs after the degreasing are mixed with the solution of 5%-10% concentration, mix; (2) homogeneous pre-treatment: adopt clarifixator that feed liquid is handled twice, soybean-drag fiber is fully ground a large amount of strippings of albumen; (3) clean: clean up with the bean dregs of clear water after to homogeneous;
2, enzymolysis-washing: (1) transfers liquid: the bean dregs behind the homogeneous are mixed with the solution of 5%-10% concentration, mix; (2) enzymolysis: the 0.1%-0.5% ratio in solid bean dregs weight is added proteolytic enzyme, enzymolysis 2.0-3.0 hour; (3) washing: the bean dregs behind enzymolysis are washed, the centrifuge dripping soybean-drag fiber;
3, high temperature weak acid extracts: (1) transfers liquid: soybean-drag fiber is mixed with the soybean-drag fiber solution that concentration is 5%-10%, mixes; (2) transfer pH: regulate the soybean-drag fiber pH value of solution to 2.8-5.0; (3) high temperature extraction: in temperature is to extract 1.5-2.0 hour under 100 ℃ of-130 ℃ of conditions;
4, centrifugal: as will to extract the back feed liquid under 5000 rev/mins-7000 rev/mins rotating speed centrifugal 10 minutes, and get supernatant liquor;
5, precipitation desalination: in supernatant liquor, add precipitation agent to precipitating fully, reach the purpose of complete desalination;
6, membrane filtration purifying: supernatant liquor is crossed 0.45 micrometer fibers film, purifying soybean polysaccharide;
7, concentrate: adopt vacuum rotation thickening equipment that soybean polysaccharide solution is concentrated, being concentrated into soybean polysaccharide concentration is 10%-15%;
8, spraying drying: concentrated solution carries out spraying drying, and inlet temperature is 210 ℃-220 ℃, and air outlet temperature is controlled at 90 ℃-100 ℃.
9, detect: the index of detection comprises physical and chemical index and biochemical indicator;
10, packing.
Advantage of the present invention is: 1, improve the purity of soybean polysaccharide, reduce the colour-change of soybean polysaccharide simultaneously, omit soybean polysaccharide decolouring step, improved production efficiency greatly, reduced production cost, reduced environmental pollution; 2, extract soybean polysaccharide under weak acid, hot conditions, the extraction yield of soybean polysaccharide improves greatly.The difference of present method is to use edible acid to reconcile the pH value, extracts soybean polysaccharide under hot conditions, promotes that soybean polysaccharide is dissolved in rapidly under hot conditions in the water, improves the extraction yield of soybean polysaccharide; 3, utilize high temperature steaming that the unpleasant odor in the raw material is decomposed or evaporation, the smell of product is accepted by the human consumer easily; 4, present method adopts the precipitation desalting technology that extracting solution is precipitated desalination; 5, the production method of this water soluble soybean polysaccharide is by centrifugation water soluble soybean polysaccharide to be separated on the basis of homogeneous pre-treatment, weak acid, high temperature steaming, precipitation desalination, membrane separation purification, is dried powdered by spraying drying at last; 6, production technique realizes industrialization simply, easily.
Embodiment
Embodiment one, be raw material with bean dregs, its processing step is:
1, the bean dregs homogeneous that forms of bean product processing back: (1) transfers liquid: bean dregs after the degreasing are mixed with the solution of 5%-concentration, mix; (2) homogeneous pre-treatment: adopt clarifixator that feed liquid is handled twice, soybean-drag fiber is fully ground a large amount of strippings of albumen; (3) clean: clean up with the bean dregs of clear water after to homogeneous;
2, enzymolysis-washing: (1) transfers liquid: the bean dregs behind the homogeneous are mixed with the solution of 5% concentration, mix; (2) enzymolysis: 0.1% ratio in solid bean dregs weight is added proteolytic enzyme, enzymolysis 2.0 hours; (3) washing: the bean dregs behind enzymolysis are washed, the centrifuge dripping soybean-drag fiber;
3, high temperature weak acid extracts: (1) transfers liquid: it is 5% soybean-drag fiber solution that soybean-drag fiber is mixed with concentration, mixes; (2) transfer pH: regulate soybean-drag fiber pH value of solution to 2.8; (3) high temperature extraction: in temperature is to extract 1.5 hours under 100 ℃ of conditions;
4, centrifugal: as will to extract the back feed liquid under 5000 rev/mins rotating speed centrifugal 10 minutes, and get supernatant liquor;
5, precipitation desalination: in supernatant liquor, add precipitation agent to precipitating fully, reach the purpose of complete desalination;
6, membrane filtration purifying: supernatant liquor is crossed 0.45 micrometer fibers film, purifying soybean polysaccharide;
7, concentrate: adopt vacuum rotation thickening equipment that soybean polysaccharide solution is concentrated, being concentrated into soybean polysaccharide concentration is 10%;
8, spraying drying: concentrated solution carries out spraying drying, and inlet temperature is 210 ℃, and air outlet temperature is controlled at 90 ℃.
9, detect: the index of detection comprises physical and chemical index and biochemical indicator;
10, packing.
Embodiment two, be raw material with bean dregs, its processing step is:
1, the bean dregs homogeneous that forms of bean product processing back: (1) transfers liquid: bean dregs after the degreasing are mixed with the solution of 10% concentration, mix; (2) homogeneous pre-treatment: adopt clarifixator that feed liquid is handled twice, soybean-drag fiber is fully ground a large amount of strippings of albumen; (3) clean: clean up with the bean dregs of clear water after to homogeneous;
2, enzymolysis-washing: (1) transfers liquid: the bean dregs behind the homogeneous are mixed with the solution of 10% concentration, mix; (2) enzymolysis: the 0.1%-0.5% ratio in solid bean dregs weight is added proteolytic enzyme, enzymolysis 3.0 hours; (3) washing: the bean dregs behind enzymolysis are washed, the centrifuge dripping soybean-drag fiber;
3, high temperature weak acid extracts: (1) transfers liquid: it is 10% soybean-drag fiber solution that soybean-drag fiber is mixed with concentration, mixes; (2) transfer pH: regulate soybean-drag fiber pH value of solution to 5.0; (3) high temperature extraction: in temperature is to extract 2.0 hours under 130 ℃ of conditions;
4, centrifugal: as will to extract the back feed liquid under 7000 rev/mins rotating speed centrifugal 10 minutes, and get supernatant liquor;
5, precipitation desalination: in supernatant liquor, add precipitation agent to precipitating fully, reach the purpose of complete desalination;
6, membrane filtration purifying: supernatant liquor is crossed 0.45 micrometer fibers film, purifying soybean polysaccharide;
7, concentrate: adopt vacuum rotation thickening equipment that soybean polysaccharide solution is concentrated, being concentrated into soybean polysaccharide concentration is 15%;
8, spraying drying: concentrated solution carries out spraying drying, and inlet temperature is 220 ℃, and air outlet temperature is controlled at 100 ℃.
9, detect: the index of detection comprises physical and chemical index and biochemical indicator;
10, packing.
Embodiment three, be raw material with bean dregs, its processing step is:
1, the bean dregs homogeneous that forms of bean product processing back: (1) transfers liquid: bean dregs after the degreasing are mixed with the solution of 8% concentration, mix; (2) homogeneous pre-treatment: adopt clarifixator that feed liquid is handled twice, soybean-drag fiber is fully ground a large amount of strippings of albumen; (3) clean: clean up with the bean dregs of clear water after to homogeneous;
2, enzymolysis-washing: (1) transfers liquid: the bean dregs behind the homogeneous are mixed with the solution of 8% concentration, mix; (2) enzymolysis: 0.3% ratio in solid bean dregs weight is added proteolytic enzyme, enzymolysis 2.5 hours; (3) washing: the bean dregs behind enzymolysis are washed, the centrifuge dripping soybean-drag fiber;
3, high temperature weak acid extracts: (1) transfers liquid: it is 8% soybean-drag fiber solution that soybean-drag fiber is mixed with concentration, mixes; (2) transfer pH: regulate soybean-drag fiber pH value of solution to 4.0; (3) high temperature extraction: in temperature is to extract 1.8 hours under 120 ℃ of conditions;
4, centrifugal: as will to extract the back feed liquid under 6000 rev/mins rotating speed centrifugal 10 minutes, and get supernatant liquor;
5, precipitation desalination: in supernatant liquor, add precipitation agent to precipitating fully, reach the purpose of complete desalination;
6, membrane filtration purifying: supernatant liquor is crossed 0.45 micrometer fibers film, purifying soybean polysaccharide;
7, concentrate: adopt vacuum rotation thickening equipment that soybean polysaccharide solution is concentrated, being concentrated into soybean polysaccharide concentration is 13%;
8, spraying drying: concentrated solution carries out spraying drying, and inlet temperature is 215 ℃, and air outlet temperature is controlled at 95 ℃.
9, detect: the index of detection comprises physical and chemical index and biochemical indicator;
10, packing.
Claims (1)
1, a kind of preparation method of water soluble soybean polysaccharide is characterized in that the preparation method is:
(1) the bean dregs homogeneous that forms of bean product processing back: (1) transfers liquid: bean dregs after the degreasing are mixed with the solution of 5%-10% concentration, mix; (2) homogeneous pre-treatment: adopt clarifixator that feed liquid is handled twice, soybean-drag fiber is fully ground a large amount of strippings of albumen; (3) clean: clean up with the bean dregs of clear water after to homogeneous;
(2) enzymolysis-washing: (1) transfers liquid: the bean dregs behind the homogeneous are mixed with the solution of 5%-10% concentration, mix; (2) enzymolysis: the 0.1%-0.5% ratio in solid bean dregs weight is added proteolytic enzyme, enzymolysis 2.0-3.0 hour; (3) washing: the bean dregs behind enzymolysis are washed, the centrifuge dripping soybean-drag fiber;
(3) high temperature weak acid extracts: (1) transfers liquid: soybean-drag fiber is mixed with the soybean-drag fiber solution that concentration is 5%-10%, mixes; (2) transfer pH: regulate the soybean-drag fiber pH value of solution to 2.8-5.0; (3) high temperature extraction: in temperature is to extract 1.5-2.0 hour under 100 ℃ of-130 ℃ of conditions;
(4) centrifugal: as will to extract the back feed liquid under 5000 rev/mins-7000 rev/mins rotating speed centrifugal 10 minutes, and get supernatant liquor;
(5) precipitation desalination: in supernatant liquor, add precipitation agent to precipitating fully, reach the purpose of complete desalination;
(6) membrane filtration purifying: supernatant liquor is crossed 0.45 micrometer fibers film, purifying soybean polysaccharide;
(7) concentrate: adopt vacuum rotation thickening equipment that soybean polysaccharide solution is concentrated, being concentrated into soybean polysaccharide concentration is 10%-15%;
(8) spraying drying: concentrated solution carries out spraying drying, and inlet temperature is 210 ℃-220 ℃, and air outlet temperature is controlled at 90 ℃-100 ℃.
(9) detect: the index of detection comprises physical and chemical index and biochemical indicator;
(10) packing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910186212A CN101671400A (en) | 2009-10-13 | 2009-10-13 | Method of preparing soluble soybean polysaccharide |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910186212A CN101671400A (en) | 2009-10-13 | 2009-10-13 | Method of preparing soluble soybean polysaccharide |
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| CN101671400A true CN101671400A (en) | 2010-03-17 |
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| CN200910186212A Pending CN101671400A (en) | 2009-10-13 | 2009-10-13 | Method of preparing soluble soybean polysaccharide |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250260A (en) * | 2010-05-21 | 2011-11-23 | 上海易初生物工程有限公司 | Enzyme engineering manufacturing method of functional glycan |
| CN102558377A (en) * | 2011-12-23 | 2012-07-11 | 南昌大学 | Preparation method of soybean polysaccharide gum |
| CN102550982A (en) * | 2011-12-28 | 2012-07-11 | 华东师范大学 | Method for preparing foam stabilized soybean fibers |
| CN108976305A (en) * | 2017-05-31 | 2018-12-11 | 宋鸣宇 | A kind of solubility amidation soybean polyoses and preparation method thereof |
| CN112851828A (en) * | 2021-01-14 | 2021-05-28 | 江南大学 | Preparation method and application of soluble soybean polysaccharide for stabilizing acidic whey protein beverage |
-
2009
- 2009-10-13 CN CN200910186212A patent/CN101671400A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250260A (en) * | 2010-05-21 | 2011-11-23 | 上海易初生物工程有限公司 | Enzyme engineering manufacturing method of functional glycan |
| CN102250260B (en) * | 2010-05-21 | 2015-06-03 | 上海和实生物科技有限公司 | Enzyme engineering manufacturing method of functional glycan |
| CN102558377A (en) * | 2011-12-23 | 2012-07-11 | 南昌大学 | Preparation method of soybean polysaccharide gum |
| CN102550982A (en) * | 2011-12-28 | 2012-07-11 | 华东师范大学 | Method for preparing foam stabilized soybean fibers |
| CN102550982B (en) * | 2011-12-28 | 2013-07-17 | 华东师范大学 | Method for preparing foam stabilized soybean fibers |
| CN108976305A (en) * | 2017-05-31 | 2018-12-11 | 宋鸣宇 | A kind of solubility amidation soybean polyoses and preparation method thereof |
| CN112851828A (en) * | 2021-01-14 | 2021-05-28 | 江南大学 | Preparation method and application of soluble soybean polysaccharide for stabilizing acidic whey protein beverage |
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Open date: 20100317 |