CN102192944B - Linear scanning stripping voltammetry method for detecting content of vitamins in blood sample - Google Patents
Linear scanning stripping voltammetry method for detecting content of vitamins in blood sample Download PDFInfo
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- CN102192944B CN102192944B CN 201110070638 CN201110070638A CN102192944B CN 102192944 B CN102192944 B CN 102192944B CN 201110070638 CN201110070638 CN 201110070638 CN 201110070638 A CN201110070638 A CN 201110070638A CN 102192944 B CN102192944 B CN 102192944B
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- Prior art keywords
- vitamin
- perchlorate
- sample
- blood sample
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- 229940088594 vitamin Drugs 0.000 title claims abstract description 61
- 229930003231 vitamin Natural products 0.000 title claims abstract description 61
- 235000013343 vitamin Nutrition 0.000 title claims abstract description 61
- 239000011782 vitamin Substances 0.000 title claims abstract description 61
- 239000008280 blood Substances 0.000 title claims abstract description 55
- 210000004369 blood Anatomy 0.000 title claims abstract description 55
- 238000003950 stripping voltammetry Methods 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title abstract description 13
- 239000000523 sample Substances 0.000 claims abstract description 110
- 150000003722 vitamin derivatives Chemical class 0.000 claims abstract description 55
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims abstract description 44
- 229930003427 Vitamin E Natural products 0.000 claims abstract description 22
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims abstract description 22
- 235000019165 vitamin E Nutrition 0.000 claims abstract description 22
- 239000011709 vitamin E Substances 0.000 claims abstract description 22
- 229940046009 vitamin E Drugs 0.000 claims abstract description 22
- 238000006479 redox reaction Methods 0.000 claims abstract description 3
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims description 48
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 claims description 44
- 239000002253 acid Substances 0.000 claims description 30
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 28
- 238000004070 electrodeposition Methods 0.000 claims description 26
- VLTRZXGMWDSKGL-UHFFFAOYSA-M perchlorate Inorganic materials [O-]Cl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-M 0.000 claims description 24
- 239000000203 mixture Substances 0.000 claims description 22
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 20
- 230000000284 resting effect Effects 0.000 claims description 13
- 238000012360 testing method Methods 0.000 claims description 13
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 10
- 229910017604 nitric acid Inorganic materials 0.000 claims description 10
- AXZAYXJCENRGIM-UHFFFAOYSA-J dipotassium;tetrabromoplatinum(2-) Chemical compound [K+].[K+].[Br-].[Br-].[Br-].[Br-].[Pt+2] AXZAYXJCENRGIM-UHFFFAOYSA-J 0.000 claims description 9
- 229910001487 potassium perchlorate Inorganic materials 0.000 claims description 9
- HHEFNVCDPLQQTP-UHFFFAOYSA-N ammonium perchlorate Chemical compound [NH4+].[O-]Cl(=O)(=O)=O HHEFNVCDPLQQTP-UHFFFAOYSA-N 0.000 claims description 8
- MHCFAGZWMAWTNR-UHFFFAOYSA-M lithium perchlorate Chemical compound [Li+].[O-]Cl(=O)(=O)=O MHCFAGZWMAWTNR-UHFFFAOYSA-M 0.000 claims description 8
- 229910001486 lithium perchlorate Inorganic materials 0.000 claims description 8
- BAZAXWOYCMUHIX-UHFFFAOYSA-M sodium perchlorate Chemical compound [Na+].[O-]Cl(=O)(=O)=O BAZAXWOYCMUHIX-UHFFFAOYSA-M 0.000 claims description 8
- 229910001488 sodium perchlorate Inorganic materials 0.000 claims description 8
- RBWNDBNSJFCLBZ-UHFFFAOYSA-N 7-methyl-5,6,7,8-tetrahydro-3h-[1]benzothiolo[2,3-d]pyrimidine-4-thione Chemical compound N1=CNC(=S)C2=C1SC1=C2CCC(C)C1 RBWNDBNSJFCLBZ-UHFFFAOYSA-N 0.000 claims description 7
- MPCRDALPQLDDFX-UHFFFAOYSA-L Magnesium perchlorate Chemical compound [Mg+2].[O-]Cl(=O)(=O)=O.[O-]Cl(=O)(=O)=O MPCRDALPQLDDFX-UHFFFAOYSA-L 0.000 claims description 7
- 230000005477 standard model Effects 0.000 claims description 7
- 239000012530 fluid Substances 0.000 claims description 4
- 238000001514 detection method Methods 0.000 abstract description 20
- 230000035945 sensitivity Effects 0.000 abstract description 14
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 abstract description 8
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 abstract description 6
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 abstract description 5
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 abstract description 5
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 abstract description 5
- 235000019155 vitamin A Nutrition 0.000 abstract description 5
- 239000011719 vitamin A Substances 0.000 abstract description 5
- 229940045997 vitamin a Drugs 0.000 abstract description 5
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 abstract description 4
- ABSPRNADVQNDOU-UHFFFAOYSA-N Menaquinone 1 Natural products C1=CC=C2C(=O)C(CC=C(C)C)=C(C)C(=O)C2=C1 ABSPRNADVQNDOU-UHFFFAOYSA-N 0.000 abstract description 4
- 229930003268 Vitamin C Natural products 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 4
- 235000019175 phylloquinone Nutrition 0.000 abstract description 4
- 239000011772 phylloquinone Substances 0.000 abstract description 4
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 abstract description 4
- MBWXNTAXLNYFJB-NKFFZRIASA-N phylloquinone Chemical compound C1=CC=C2C(=O)C(C/C=C(C)/CCC[C@H](C)CCC[C@H](C)CCCC(C)C)=C(C)C(=O)C2=C1 MBWXNTAXLNYFJB-NKFFZRIASA-N 0.000 abstract description 4
- 229960001898 phytomenadione Drugs 0.000 abstract description 4
- 235000019154 vitamin C Nutrition 0.000 abstract description 4
- 239000011718 vitamin C Substances 0.000 abstract description 4
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 abstract description 3
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 abstract description 3
- 229930003451 Vitamin B1 Natural products 0.000 abstract description 3
- 229930003471 Vitamin B2 Natural products 0.000 abstract description 3
- 229930003316 Vitamin D Natural products 0.000 abstract description 3
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 abstract description 3
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 abstract description 3
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 abstract description 3
- 229960002477 riboflavin Drugs 0.000 abstract description 3
- 229960003495 thiamine Drugs 0.000 abstract description 3
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 abstract description 3
- 235000010374 vitamin B1 Nutrition 0.000 abstract description 3
- 239000011691 vitamin B1 Substances 0.000 abstract description 3
- 235000019164 vitamin B2 Nutrition 0.000 abstract description 3
- 239000011716 vitamin B2 Substances 0.000 abstract description 3
- 235000019158 vitamin B6 Nutrition 0.000 abstract description 3
- 239000011726 vitamin B6 Substances 0.000 abstract description 3
- 235000019166 vitamin D Nutrition 0.000 abstract description 3
- 239000011710 vitamin D Substances 0.000 abstract description 3
- 150000003710 vitamin D derivatives Chemical class 0.000 abstract description 3
- 229940011671 vitamin b6 Drugs 0.000 abstract description 3
- 229940046008 vitamin d Drugs 0.000 abstract description 3
- 238000004458 analytical method Methods 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 abstract description 2
- MJVAVZPDRWSRRC-UHFFFAOYSA-N Menadione Chemical compound C1=CC=C2C(=O)C(C)=CC(=O)C2=C1 MJVAVZPDRWSRRC-UHFFFAOYSA-N 0.000 abstract 2
- 229930003779 Vitamin B12 Natural products 0.000 abstract 1
- 229930003761 Vitamin B9 Natural products 0.000 abstract 1
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 abstract 1
- 235000019163 vitamin B12 Nutrition 0.000 abstract 1
- 239000011715 vitamin B12 Substances 0.000 abstract 1
- 235000019159 vitamin B9 Nutrition 0.000 abstract 1
- 239000011727 vitamin B9 Substances 0.000 abstract 1
- 235000012711 vitamin K3 Nutrition 0.000 abstract 1
- 239000011652 vitamin K3 Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 26
- 239000004094 surface-active agent Substances 0.000 description 11
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 10
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 8
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 7
- 229940095064 tartrate Drugs 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 6
- 239000008351 acetate buffer Substances 0.000 description 6
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 6
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 description 5
- 239000001103 potassium chloride Substances 0.000 description 5
- 235000011164 potassium chloride Nutrition 0.000 description 5
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 4
- OKIZCWYLBDKLSU-UHFFFAOYSA-M N,N,N-Trimethylmethanaminium chloride Chemical compound [Cl-].C[N+](C)(C)C OKIZCWYLBDKLSU-UHFFFAOYSA-M 0.000 description 4
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 4
- 235000019270 ammonium chloride Nutrition 0.000 description 4
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- JGUQDUKBUKFFRO-CIIODKQPSA-N dimethylglyoxime Chemical compound O/N=C(/C)\C(\C)=N\O JGUQDUKBUKFFRO-CIIODKQPSA-N 0.000 description 4
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- -1 neopelex Chemical compound 0.000 description 3
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- 239000010452 phosphate Substances 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
- AVTYONGGKAJVTE-OLXYHTOASA-L potassium L-tartrate Chemical compound [K+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O AVTYONGGKAJVTE-OLXYHTOASA-L 0.000 description 3
- 239000004323 potassium nitrate Substances 0.000 description 3
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- VZOPRCCTKLAGPN-ZFJVMAEJSA-L potassium;sodium;(2r,3r)-2,3-dihydroxybutanedioate;tetrahydrate Chemical compound O.O.O.O.[Na+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O VZOPRCCTKLAGPN-ZFJVMAEJSA-L 0.000 description 3
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- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 2
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 2
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
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- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
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- 229910052742 iron Inorganic materials 0.000 description 1
- 229910000398 iron phosphate Inorganic materials 0.000 description 1
- WBJZTOZJJYAKHQ-UHFFFAOYSA-K iron(3+) phosphate Chemical compound [Fe+3].[O-]P([O-])([O-])=O WBJZTOZJJYAKHQ-UHFFFAOYSA-K 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000019691 monocalcium phosphate Nutrition 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 208000007442 rickets Diseases 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
Images
Abstract
The invention relates to a linear scanning stripping voltammetry method for detecting content of vitamins in a blood sample, which belongs to the technical field of vitamin analysis and detection. The linear scanning stripping voltammetry method for detecting the content of the vitamins in the blood sample is characterized by comprising the following steps of: mixing a blood sample to be tested and vitamin sample processing liquid, and detecting a current signal which is generated when the sample is subjected to oxidation-reduction reaction on the sensor probe of a vitamin detector by the linear scanning stripping voltammetry method; and making a standard curve by comparing the current signal values of standard vitamin samples at different concentrations, and obtaining the content of the vitamins in the blood sample to be tested according to the current signal value generated by the sample to be tested. The method has the advantages that: the method is high in sensitivity and accuracy, convenient to operate and quick in operation, has a wide application range, is suitable for medical health departments and can be applied to the quick detection of the content of the vitamins in theblood sample, vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B9, vitamin B12, vitamin C, vitamin D, vitamin E, vitamin K1 and vitamin K3 in the blood sample can be analyzed and detected, and the like.
Description
Technical field
The invention belongs to the vitamin technical field of analysis and detection, particularly relate to a kind of linear sweep stripping voltammetry that detects the blood sample vitamin content.
Background technology
Vitamin (vitamin) be body be keep normal physiological function must be by a class micro-content organism of food intake, regulating metabolism and keeping aspect such as physiological function and bringing into play important effect.Long-term lacking or certain vitamin of excessive absorption all can cause corresponding disease.As yctalopia, scheroma and dry skin can appear in the A that is deficient in vitamin; The D that is deficient in vitamin can suffer from rickets; The B12 that is deficient in vitamin can suffer from pernicious anaemia; The vitamin E of excess intake can destroy coagulation function, increases hemorrhage possibility; Taking in the vitamin C that surpasses 1000 milligrams can influence the functions of expelling toxin of kidney.
At present, instrument and the detection method of human body vitamin content are had nothing in common with each other, and mainly contain microbial method, ultraviolet spectrophotometry, fluorometry, high performance liquid chromatography etc.Loaded down with trivial details consuming time, the more organic solvent of needs of pretreatment technology in the high performance liquid chromatography, and the serum requirement is bigger.The vitamin kind that ultraviolet spectrophotometry, fluorometry can detect is less.The instrument that is applied to the vitamin detection at present has: Korea S Younglin company has developed vitameter, utilizes the various vitamins in efficient liquid phase process detection food, the medicine; Germany visits a R-Biopharm company and utilizes fluorescence method to produce the instrument that detects vitamin.Because instruments such as high performance liquid chromatograph, fluorescence analyser are expensive, and need the technical skill personnel to operate, detection method is numerous and diverse, and detection time is longer, so be difficult for promoting.
Summary of the invention
The present invention provides a kind of linear sweep stripping voltammetry that detects the blood sample vitamin content for solving the technical matters that exists in the known technology.
The purpose of this invention is to provide the vitamin electrochemical analysis method in a kind of blood sample, have highly sensitive, characteristics such as accuracy good, easy and simple to handle, quick, applied range, be suitable for medical department and use, can carry out the linear sweep stripping voltammetry of the detection blood sample vitamin content of characteristics such as analyzing and testing to the vitamin A in the blood sample, B1, B2, B6, B9, B12, C, D, E, K1, K3.
Implementation procedure of the present invention: get a certain amount of blood sample and mix with a certain amount of homemade vitamin sample treating fluid, utilize the linear sweep stripping voltammetry to make the vitamin in the blood sample at homemade sensor probe oxidation (reduction) reaction take place, produce current signal.By comparing the current signal value of variable concentrations vitamin standard model, formulate typical curve, according to the current signal value that testing sample produces, obtain the content of vitamin in the testing sample.
The linear sweep stripping voltammetry that the present invention detects the blood sample vitamin content for the technical scheme that solves the technical matters that exists in the known technology and take is:
A kind of linear sweep stripping voltammetry that detects the blood sample vitamin content, be characterized in: get blood sample to be measured and mix with vitamin sample treating fluid, the current signal that utilizes linear sweep stripping voltammetry test sample when redox reaction takes place vitamin detector sensor probe electrode, to produce; By comparing the current signal value of variable concentrations vitamin standard model, the typical curve of formulation according to the current signal value that testing sample produces, obtains the content of vitamin in the blood sample to be measured.
The present invention detects the linear sweep stripping voltammetry of blood sample vitamin content can also take following technical scheme:
The linear sweep stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin A content, the enrichment of detector (electro-deposition) current potential is-200～400mV, and the enrichment electrodeposition time is 10～90s, and initial potential is-200～400mV, the termination current potential is 1000～1800mV, sweep velocity is 20～150mV/s, and the sample interval is 4～20mV, and be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 0.01～50mA.The vitamin A sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is that one or more are formed in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, and the addition of perchlorate is 0.05～10mol/L; Sodium perchlorate, potassium perchlorate, lithium perchlorate, ammonia perchlorate be perchlorate most preferably.Strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～5mol/L; Nitric acid, perchloric acid be strong acid most preferably.The addition of dimethyl formamide is 1～100 mmol/L.
The linear sweep stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin B1 content, the enrichment of detector (electro-deposition) current potential is-400～400mV, and the enrichment electrodeposition time is 10～60s, and initial potential is-200～400mV, the termination current potential is 1000～1800mV, sweep velocity is 20～150mV/s, and the sample interval is 4～20mV, and be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 0.01～50mA.The vitamin B1 sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is that one or more are formed in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, and the addition of perchlorate is 0.1～10mol/L; Strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～8mol/L; The addition of dimethyl formamide is 0.01～2mmol/L.
The linear sweep stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin B2 content, the enrichment of detector (electro-deposition) current potential is-1500～-500mV, the enrichment electrodeposition time is 20～80s, initial potential is-1500～-500mV, the termination current potential is-400～900mV, sweep velocity is 20～150mV/s, the sample interval is 4～20mV, be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s, and range (sensitivity) is 0.01～50mA.The vitamin B2 sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is that one or more are formed in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, and the addition of perchlorate is 0.05～10mol/L; Strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～2mol/L; The addition of dimethyl formamide is 0.01～10mmol/L.
The linear sweep stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin B6 content, the enrichment of detector (electro-deposition) current potential is-600～400mV, and the enrichment electrodeposition time is 10～60s, and initial potential is-600～400mV, the termination current potential is 500～1000mV, sweep velocity is 20～150mV/s, and the sample interval is 4～20mV, and be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 0.01～50mA.The vitamin B6 sample treatment solution is made up of phosphate buffered solution, alkaline solution, surfactant; Phosphate buffered solution is by sodium hydrogen phosphate, sodium dihydrogen phosphate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, phosphoric acid one hydrogen iron, primary iron phosphate, diammonium hydrogen phosphate, ammonium dihydrogen phosphate (ADP), one or more compositions in the calcium dihydrogen phosphate, the phosphate buffered solution addition is 0.005～10mol/L, alkaline solution is by NaOH, potassium hydroxide, calcium hydroxide, one or more compositions in the ammoniacal liquor, the alkaline solution addition is 0.01～1mol/L, surfactant is by stearic acid, neopelex, cetyl trimethyl ammonium bromide, glycerin monostearate, the fatty acid sorb is smooth, one or more compositions of polysorbate, the surfactant addition is 0.05～10mmol/L, and the fatty acid sorb is smooth, polysorbate, glycerin monostearate be surfactant most preferably.
The linear sweep stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample Cobastab 9 content, the enrichment of detector (electro-deposition) current potential is-2000～-500mV, the enrichment electrodeposition time is 10～90s, initial potential is-2000～-1000mV, the termination current potential is-400～1000mV, sweep velocity is 20～150mV/s, and the sample interval is 4～20mV, and be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 0.01～50mA.Cobastab 9 sample treatment solutions are made up of tartrate, acetate buffer solution, surfactant, ionic strength adjustor, strong acid and dimethylglyoxime ethanolic solution; Tartrate is by sodium potassium tartrate tetrahydrate, potassium antimony tartrate, tartrate ammonia potassium, sodium tartrate, one or more compositions in the potassium tartrate, potassium tartrate, sodium potassium tartrate tetrahydrate be tartrate most preferably, the tartrate addition is 2～10mmol/L, acetate buffer solution is by sodium acetate, potassium acetate, one or more are formed with acetic acid in the ammonium acetate, the acetate buffer solution addition is 0.01～10mol/L, surfactant is by stearic acid, neopelex, cetyl trimethyl ammonium bromide, glycerin monostearate, the fatty acid sorb is smooth, one or more compositions in the polysorbate, cetyl trimethyl ammonium bromide, glycerin monostearate be surfactant most preferably, the surfactant addition is 0.05～50mmol/L, ionic strength adjustor is by potassium chloride, sodium chloride, sodium sulphate, potassium chloride, one or more compositions in the potassium nitrate, the ionic strength adjustor addition is 0.01～10mol/L, strong acid is by hydrochloric acid, sulfuric acid, nitric acid, one or more compositions in the perchloric acid, sodium chloride, potassium chloride, potassium nitrate be ionic strength adjustor most preferably, the strong acid addition is 0.01～5mol/L, and the addition of dimethylglyoxime is 0.01～10mmol/L.
The linear sweep stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample cobalamin content, the enrichment of detector (electro-deposition) current potential is-2000～-500mV, the enrichment electrodeposition time is 10～90s, initial potential is-2000～-1000mV, the termination current potential is-400～1000mV, sweep velocity is 20～150mV/s, and the sample interval is 4～20mV, and be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 0.01～50mA.The cobalamin sample treatment solution is made up of tartrate, acetate buffer solution, surfactant, ionic strength adjustor, strong acid, ethanol and dimethylglyoxime; Tartrate is by sodium potassium tartrate tetrahydrate, potassium antimony tartrate, tartrate ammonia potassium, sodium tartrate, one or more compositions in the potassium tartrate, the tartrate addition is 0.02～5mol/L, acetate buffer solution is by by sodium acetate, potassium acetate, one or more are formed with acetic acid in the ammonium acetate, the acetate buffer solution addition is 0.01～10mol/L, surfactant is by stearic acid, neopelex, cetyl trimethyl ammonium bromide, glycerin monostearate, the fatty acid sorb is smooth, one or more compositions in the polysorbate, the surfactant addition is 0.02～10mmol/L, ionic strength adjustor is by potassium chloride, sodium chloride, sodium sulphate, potassium chloride, one or more compositions in the potassium nitrate, the ionic strength adjustor addition is 0.05～10mol/L, strong acid is by hydrochloric acid, sulfuric acid, nitric acid, one or more compositions in the perchloric acid, the strong acid addition is 0.01～5mol/L, and the addition of dimethylglyoxime is 0.02～10mmol/L.
The linear sweep stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample Vitamin C content, the enrichment of detector (electro-deposition) current potential is-400～400mV, and the enrichment electrodeposition time is 10～60s, and initial potential is-200～400mV, the termination current potential is 1000～1800mV, sweep velocity is 20～150mV/s, and the sample interval is 4～20mV, and be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 0.01～50mA.The vitamin C sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is one or more compositions in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, the perchlorate addition is 0.01～10 mol/L, strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～10mol/L; The addition of dimethyl formamide is 0.01～10mmol/L.
The linear sweep stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin D content, the enrichment of detector (electro-deposition) current potential is-200～400mV, and the enrichment electrodeposition time is 10～90s, and initial potential is-200～400mV, the termination current potential is 1000～1800mV, sweep velocity is 20～150mV/s, and the sample interval is 4～20mV, and be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 0.01～50mA.The vitamin D sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is one or more compositions in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, the perchlorate addition is 0.04～8 mol/L, strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.02～10mol/L; The addition of dimethyl formamide is 0.05～10mmol/L;
The linear sweep stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample content of vitamin E, the enrichment of detector (electro-deposition) current potential is-200～400mV, and the enrichment electrodeposition time is 10～90s, and initial potential is-200～400mV, the termination current potential is 1000～1800mV, sweep velocity is 20～150mV/s, and the sample interval is 4～20mV, and be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 0.01～50mA.The vitamin E sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is one or more compositions in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, the perchlorate addition is 0.04～8 mol/L, strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.05～10mol/L; The addition of dimethyl formamide is 0.01～2mmol/L.
The linear sweep stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin K1 content, the enrichment of detector (electro-deposition) current potential is 100～1000mV, and the enrichment electrodeposition time is 20～80s, and initial potential is-1000～100mV, the termination current potential is-100～1500mV, sweep velocity is 20～150mV/s, and the sample interval is 4～20mV, and be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 0.01～50mA.The vitamin K1 sample treatment solution is made up of in ammonium chloride, ammonium nitrate, ammoniacal liquor, hydrochloric acid, tetramethyl ammonium chloride, diethylamine, the triethylamine one or more, ammonium chloride, hydrochloric acid, tetramethyl ammonium chloride be the vitamin K1 sample treatment solution most preferably, addition is 0.02～15mol/L;
The linear sweep stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample prokeyvit content, the enrichment of detector (electro-deposition) current potential is 100～1000mV, and the enrichment electrodeposition time is 20～80s, and initial potential is-1000～100mV, the termination current potential is-100～1500mV, sweep velocity is 20～150mV/s, and the sample interval is 4～20mV, and be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 0.01～50mA.The prokeyvit sample treatment solution is made up of in ammonium chloride, ammonium nitrate, ammoniacal liquor, hydrochloric acid, tetramethyl ammonium chloride, diethylamine, the triethylamine one or more, ammonium chloride, hydrochloric acid, tetramethyl ammonium chloride be the prokeyvit sample treatment solution most preferably, addition is 0.01～5mol/L.
Advantage and good effect that the present invention has are:
Detect the linear sweep stripping voltammetry of blood sample vitamin content owing to adopted brand-new technology scheme of the present invention, compared with prior art, that the present invention has is highly sensitive, accuracy good, easy and simple to handle, quick, applied range, be suitable for medical department and use, can carry out advantages such as analyzing and testing to the vitamin A in the blood sample, B1, B2, B6, B9, B12, C, D, E, K1, K3.
A kind of method that detects vitamin content in the blood sample provided by the invention has short, advantage such as accuracy is high, sensing range is wide of test duration, can be used for the fast detecting of blood sample vitamin content.
Description of drawings
Fig. 1 is vitamin E standard model current value of the present invention-concentration standard curve synoptic diagram.
Embodiment
For further understanding technology contents of the present invention, characteristics and effect, exemplify following examples now, and conjunction with figs. is described in detail as follows:
Consult accompanying drawing 1.
Embodiment 1
Detect the linear sweep stripping voltammetry of blood sample vitamin content: get a certain amount of testing sample and mix with a certain amount of homemade vitamin sample treating fluid, utilize the linear sweep stripping voltammetry to make the vitamin in the testing sample at homemade sensor probe oxidation (reduction) reaction take place, produce current signal.By comparing the current signal value of variable concentrations vitamin standard model, formulate typical curve, according to the current signal value that testing sample produces, obtain the content of vitamin in the testing sample.
Detect the specific implementation process of content of vitamin E in the blood sample:
1. prepare the vitamin E sample treatment solution: the vitamin E sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; The high chlorine of perchlorate is sour sodium, potassium perchlorate, and addition is 0.04～8 mol/L, and strong acid is hydrochloric acid, sulfuric acid, and addition is 0.05～10mol/L; The addition of dimethyl formamide is 1mmol/L.Range (sensitivity) is 0.01～50mA.
2. debugging detector: when detecting in the blood sample content of vitamin E, the enrichment of detector (electro-deposition) current potential is 200mV, the enrichment electrodeposition time is 50s, initial potential is 200mV, and the termination current potential is 1500mV, and sweep velocity is 90mV/s, the sample interval is 10mV, be 40s rest time, and resting potential is 300mV, and the stop time is 30s; Range (sensitivity) is 0.01～50mA.
3, vitamin E standard model-concentration standard curve
(1) accurately pipettes 2000uL vitamin E sample treatment solution with pipettor;
(2) accurately pipette 10uL, 20uL, 50uL, 750uL, 100uL vitamin E standard model respectively to the vitamin E sample treatment solution with pipettor, mix;
(3) work for the treatment of electrode and auxiliary electrode under the condition of the vitamin E detected parameters that has configured, detect the record current signal value respectively;
(4) draw vitamin E standard model current value-concentration standard curve (accompanying drawing).
4, the method for quick of vitamin E in the blood sample
(1) accurately pipettes 2000uL vitamin E sample treatment solution with pipettor;
(2) accurately pipette the 80uL blood sample to the vitamin E sample treatment solution with pipettor, mix;
(3 work for the treatment of electrode and auxiliary electrodes under the condition of the vitamin E detected parameters that has configured, detect the record current signal value;
(4) compare with typical curve, obtain the content of vitamin E in the blood sample.
Claims (1)
1. linear sweep stripping voltammetry that detects the blood sample vitamin content, it is characterized in that: get blood sample to be measured and mix with vitamin sample treating fluid, the current signal that utilizes linear sweep stripping voltammetry test sample when redox reaction takes place vitamin detector sensor probe, to produce; By comparing the current signal value of variable concentrations vitamin standard model, formulate typical curve, according to the current signal value that testing sample produces, obtain the content of vitamin in the blood sample to be measured;
When detecting in the blood sample content of vitamin E, the enrichment electro-deposition current potential of detector is-200～400mV, the enrichment electrodeposition time is 10～90s, initial potential is-200～400mV, and the termination current potential is 1000～1800mV, and sweep velocity is 20～150mV/s, the sample interval is 4～20mV, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; The vitamin E sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is one or more compositions in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, the perchlorate addition is 0.04～8 mol/L, strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.05～10mol/L; The addition of dimethyl formamide is 0.01～2mmol/L.
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| CN112557469A (en) * | 2020-12-22 | 2021-03-26 | 合肥天一生物技术研究所有限责任公司 | Convolution current voltammetry for simultaneously detecting vitamin A, D, E |
| CN112557471B (en) * | 2020-12-22 | 2023-07-18 | 合肥天一生物技术研究所有限责任公司 | Preparation method and detection method of modified electrode capable of simultaneously detecting vitamins B6 and B9 |
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