CN102221574B - Linear sweep voltammetry for detecting vitamin content of blood sample - Google Patents
Linear sweep voltammetry for detecting vitamin content of blood sample Download PDFInfo
- Publication number
- CN102221574B CN102221574B CN 201110070640 CN201110070640A CN102221574B CN 102221574 B CN102221574 B CN 102221574B CN 201110070640 CN201110070640 CN 201110070640 CN 201110070640 A CN201110070640 A CN 201110070640A CN 102221574 B CN102221574 B CN 102221574B
- Authority
- CN
- China
- Prior art keywords
- vitamin
- perchlorate
- sample
- blood sample
- content
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 229940088594 vitamin Drugs 0.000 title claims abstract description 61
- 229930003231 vitamin Natural products 0.000 title claims abstract description 61
- 235000013343 vitamin Nutrition 0.000 title claims abstract description 61
- 239000011782 vitamin Substances 0.000 title claims abstract description 61
- 150000003722 vitamin derivatives Chemical class 0.000 title claims abstract description 60
- 239000008280 blood Substances 0.000 title claims abstract description 55
- 210000004369 blood Anatomy 0.000 title claims abstract description 55
- 238000004502 linear sweep voltammetry Methods 0.000 title claims abstract description 29
- 239000000523 sample Substances 0.000 claims abstract description 109
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 40
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 20
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 20
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 20
- 239000011718 vitamin C Substances 0.000 claims abstract description 20
- 239000012530 fluid Substances 0.000 claims abstract description 5
- 238000006479 redox reaction Methods 0.000 claims abstract description 3
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims description 48
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 claims description 45
- 239000002253 acid Substances 0.000 claims description 31
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 28
- VLTRZXGMWDSKGL-UHFFFAOYSA-M perchlorate Inorganic materials [O-]Cl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-M 0.000 claims description 25
- 239000000203 mixture Substances 0.000 claims description 22
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 20
- 238000012360 testing method Methods 0.000 claims description 13
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 10
- 229910017604 nitric acid Inorganic materials 0.000 claims description 10
- AXZAYXJCENRGIM-UHFFFAOYSA-J dipotassium;tetrabromoplatinum(2-) Chemical compound [K+].[K+].[Br-].[Br-].[Br-].[Br-].[Pt+2] AXZAYXJCENRGIM-UHFFFAOYSA-J 0.000 claims description 9
- 229910001487 potassium perchlorate Inorganic materials 0.000 claims description 9
- BAZAXWOYCMUHIX-UHFFFAOYSA-M sodium perchlorate Chemical compound [Na+].[O-]Cl(=O)(=O)=O BAZAXWOYCMUHIX-UHFFFAOYSA-M 0.000 claims description 9
- 229910001488 sodium perchlorate Inorganic materials 0.000 claims description 9
- HHEFNVCDPLQQTP-UHFFFAOYSA-N ammonium perchlorate Chemical compound [NH4+].[O-]Cl(=O)(=O)=O HHEFNVCDPLQQTP-UHFFFAOYSA-N 0.000 claims description 8
- MHCFAGZWMAWTNR-UHFFFAOYSA-M lithium perchlorate Chemical compound [Li+].[O-]Cl(=O)(=O)=O MHCFAGZWMAWTNR-UHFFFAOYSA-M 0.000 claims description 8
- 229910001486 lithium perchlorate Inorganic materials 0.000 claims description 8
- RBWNDBNSJFCLBZ-UHFFFAOYSA-N 7-methyl-5,6,7,8-tetrahydro-3h-[1]benzothiolo[2,3-d]pyrimidine-4-thione Chemical compound N1=CNC(=S)C2=C1SC1=C2CCC(C)C1 RBWNDBNSJFCLBZ-UHFFFAOYSA-N 0.000 claims description 7
- MPCRDALPQLDDFX-UHFFFAOYSA-L Magnesium perchlorate Chemical compound [Mg+2].[O-]Cl(=O)(=O)=O.[O-]Cl(=O)(=O)=O MPCRDALPQLDDFX-UHFFFAOYSA-L 0.000 claims description 7
- 230000005477 standard model Effects 0.000 claims description 7
- 238000001514 detection method Methods 0.000 abstract description 22
- 230000035945 sensitivity Effects 0.000 abstract description 13
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 abstract description 8
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 abstract description 5
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 abstract description 5
- LXNHXLLTXMVWPM-UHFFFAOYSA-N Vitamin B6 Natural products CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 abstract description 5
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 abstract description 5
- 235000019155 vitamin A Nutrition 0.000 abstract description 5
- 239000011719 vitamin A Substances 0.000 abstract description 5
- 229940045997 vitamin a Drugs 0.000 abstract description 5
- ABSPRNADVQNDOU-UHFFFAOYSA-N Menaquinone 1 Natural products C1=CC=C2C(=O)C(CC=C(C)C)=C(C)C(=O)C2=C1 ABSPRNADVQNDOU-UHFFFAOYSA-N 0.000 abstract description 4
- 229930003427 Vitamin E Natural products 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 4
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 abstract description 4
- 235000019175 phylloquinone Nutrition 0.000 abstract description 4
- 239000011772 phylloquinone Substances 0.000 abstract description 4
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 abstract description 4
- 235000019165 vitamin E Nutrition 0.000 abstract description 4
- 239000011709 vitamin E Substances 0.000 abstract description 4
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 abstract description 3
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 abstract description 3
- 229930003451 Vitamin B1 Natural products 0.000 abstract description 3
- 229930003471 Vitamin B2 Natural products 0.000 abstract description 3
- 229930003316 Vitamin D Natural products 0.000 abstract description 3
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 abstract description 3
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 abstract description 3
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 abstract description 3
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 abstract description 3
- 235000010374 vitamin B1 Nutrition 0.000 abstract description 3
- 239000011691 vitamin B1 Substances 0.000 abstract description 3
- 235000019164 vitamin B2 Nutrition 0.000 abstract description 3
- 239000011716 vitamin B2 Substances 0.000 abstract description 3
- 235000019158 vitamin B6 Nutrition 0.000 abstract description 3
- 239000011726 vitamin B6 Substances 0.000 abstract description 3
- 235000019166 vitamin D Nutrition 0.000 abstract description 3
- 239000011710 vitamin D Substances 0.000 abstract description 3
- 150000003710 vitamin D derivatives Chemical class 0.000 abstract description 3
- MJVAVZPDRWSRRC-UHFFFAOYSA-N Menadione Chemical compound C1=CC=C2C(=O)C(C)=CC(=O)C2=C1 MJVAVZPDRWSRRC-UHFFFAOYSA-N 0.000 abstract 2
- 229930003779 Vitamin B12 Natural products 0.000 abstract 1
- 229930003761 Vitamin B9 Natural products 0.000 abstract 1
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 abstract 1
- MBWXNTAXLNYFJB-LKUDQCMESA-N phylloquinone Chemical compound C1=CC=C2C(=O)C(C/C=C(C)/CCCC(C)CCCC(C)CCCC(C)C)=C(C)C(=O)C2=C1 MBWXNTAXLNYFJB-LKUDQCMESA-N 0.000 abstract 1
- ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 abstract 1
- 235000019163 vitamin B12 Nutrition 0.000 abstract 1
- 239000011715 vitamin B12 Substances 0.000 abstract 1
- 235000019159 vitamin B9 Nutrition 0.000 abstract 1
- 239000011727 vitamin B9 Substances 0.000 abstract 1
- 235000012711 vitamin K3 Nutrition 0.000 abstract 1
- 239000011652 vitamin K3 Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 26
- 239000004094 surface-active agent Substances 0.000 description 11
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 10
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 8
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 229940095064 tartrate Drugs 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 6
- 239000008351 acetate buffer Substances 0.000 description 6
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 6
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 description 5
- 239000001103 potassium chloride Substances 0.000 description 5
- 235000011164 potassium chloride Nutrition 0.000 description 5
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 4
- OKIZCWYLBDKLSU-UHFFFAOYSA-M N,N,N-Trimethylmethanaminium chloride Chemical compound [Cl-].C[N+](C)(C)C OKIZCWYLBDKLSU-UHFFFAOYSA-M 0.000 description 4
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 4
- 235000019270 ammonium chloride Nutrition 0.000 description 4
- 235000014113 dietary fatty acids Nutrition 0.000 description 4
- JGUQDUKBUKFFRO-CIIODKQPSA-N dimethylglyoxime Chemical compound O/N=C(/C)\C(\C)=N\O JGUQDUKBUKFFRO-CIIODKQPSA-N 0.000 description 4
- 239000000194 fatty acid Substances 0.000 description 4
- 229930195729 fatty acid Natural products 0.000 description 4
- 150000004665 fatty acids Chemical class 0.000 description 4
- 229950008882 polysorbate Drugs 0.000 description 4
- 229920000136 polysorbate Polymers 0.000 description 4
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 4
- 229910019142 PO4 Inorganic materials 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 235000021355 Stearic acid Nutrition 0.000 description 3
- 239000012670 alkaline solution Substances 0.000 description 3
- 235000011114 ammonium hydroxide Nutrition 0.000 description 3
- 239000008366 buffered solution Substances 0.000 description 3
- 230000002950 deficient Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- -1 neopelex Chemical compound 0.000 description 3
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 3
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 3
- 239000010452 phosphate Substances 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
- MBWXNTAXLNYFJB-NKFFZRIASA-N phylloquinone Chemical compound C1=CC=C2C(=O)C(C/C=C(C)/CCC[C@H](C)CCC[C@H](C)CCCC(C)C)=C(C)C(=O)C2=C1 MBWXNTAXLNYFJB-NKFFZRIASA-N 0.000 description 3
- 229960001898 phytomenadione Drugs 0.000 description 3
- AVTYONGGKAJVTE-OLXYHTOASA-L potassium L-tartrate Chemical compound [K+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O AVTYONGGKAJVTE-OLXYHTOASA-L 0.000 description 3
- 239000004323 potassium nitrate Substances 0.000 description 3
- 235000010333 potassium nitrate Nutrition 0.000 description 3
- 239000001472 potassium tartrate Substances 0.000 description 3
- 229940111695 potassium tartrate Drugs 0.000 description 3
- 235000011005 potassium tartrates Nutrition 0.000 description 3
- VZOPRCCTKLAGPN-ZFJVMAEJSA-L potassium;sodium;(2r,3r)-2,3-dihydroxybutanedioate;tetrahydrate Chemical compound O.O.O.O.[Na+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O VZOPRCCTKLAGPN-ZFJVMAEJSA-L 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 235000002639 sodium chloride Nutrition 0.000 description 3
- 229940074446 sodium potassium tartrate tetrahydrate Drugs 0.000 description 3
- 239000008117 stearic acid Substances 0.000 description 3
- 229940046009 vitamin E Drugs 0.000 description 3
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 2
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 2
- 239000005695 Ammonium acetate Substances 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- UGSCTJJUUHWKRM-UHFFFAOYSA-L [K+].N.C(=O)([O-])C(O)C(O)C(=O)[O-].[K+] Chemical compound [K+].N.C(=O)([O-])C(O)C(O)C(=O)[O-].[K+] UGSCTJJUUHWKRM-UHFFFAOYSA-L 0.000 description 2
- 235000019257 ammonium acetate Nutrition 0.000 description 2
- 229940043376 ammonium acetate Drugs 0.000 description 2
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 description 2
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- HPNMFZURTQLUMO-UHFFFAOYSA-N diethylamine Chemical compound CCNCC HPNMFZURTQLUMO-UHFFFAOYSA-N 0.000 description 2
- 238000005558 fluorometry Methods 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 235000019837 monoammonium phosphate Nutrition 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 230000035790 physiological processes and functions Effects 0.000 description 2
- 235000011056 potassium acetate Nutrition 0.000 description 2
- IIQJBVZYLIIMND-UHFFFAOYSA-J potassium;antimony(3+);2,3-dihydroxybutanedioate Chemical compound [K+].[Sb+3].[O-]C(=O)C(O)C(O)C([O-])=O.[O-]C(=O)C(O)C(O)C([O-])=O IIQJBVZYLIIMND-UHFFFAOYSA-J 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 229960002477 riboflavin Drugs 0.000 description 2
- HELHAJAZNSDZJO-OLXYHTOASA-L sodium L-tartrate Chemical compound [Na+].[Na+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O HELHAJAZNSDZJO-OLXYHTOASA-L 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 229910052938 sodium sulfate Inorganic materials 0.000 description 2
- 235000011152 sodium sulphate Nutrition 0.000 description 2
- 239000001433 sodium tartrate Substances 0.000 description 2
- 229960002167 sodium tartrate Drugs 0.000 description 2
- 235000011004 sodium tartrates Nutrition 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- 229960003495 thiamine Drugs 0.000 description 2
- 229940011671 vitamin b6 Drugs 0.000 description 2
- 229940046008 vitamin d Drugs 0.000 description 2
- 206010013786 Dry skin Diseases 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 208000031845 Pernicious anaemia Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 description 1
- 229940062672 calcium dihydrogen phosphate Drugs 0.000 description 1
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 1
- 239000000920 calcium hydroxide Substances 0.000 description 1
- 229910001861 calcium hydroxide Inorganic materials 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 229910000388 diammonium phosphate Inorganic materials 0.000 description 1
- 235000019838 diammonium phosphate Nutrition 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000037336 dry skin Effects 0.000 description 1
- 238000000840 electrochemical analysis Methods 0.000 description 1
- 238000002795 fluorescence method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 229910000398 iron phosphate Inorganic materials 0.000 description 1
- WBJZTOZJJYAKHQ-UHFFFAOYSA-K iron(3+) phosphate Chemical compound [Fe+3].[O-]P([O-])([O-])=O WBJZTOZJJYAKHQ-UHFFFAOYSA-K 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000019691 monocalcium phosphate Nutrition 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 208000007442 rickets Diseases 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Belonging to the technical field of analytical detection of vitamin, the invention relates to linear sweep voltammetry for detecting the vitamin content of a blood sample. The linear sweep voltammetry for detecting the vitamin content of a blood sample is characterized by: mixing a blood sample to be detected and a vitamin sample treatment fluid, and detecting the current signal generated by the sample during an oxidation reduction reaction on the sensor probe of a vitamin detector with linear sweep voltammetry, establishing a standard curve by comparing the current signal values of vitamin standard samples with different concentration, and acquiring the vitamin content of the blood sample to be detected according to the current signal values generated by the sample to be detected. The present invention has the advantages of high sensitivity, high accuracy, simple operation, rapidness, wide application scope, applicability to the health sector, and capability for analytical detection of vitamin A, B1, B2, B6, B9, B12, C, D, E, K1 and K3, thus being suitable for the rapid detection of vitamin content in a blood sample.
Description
Technical field
The invention belongs to the vitamin technical field of analysis and detection, particularly relate to a kind of linear sweep voltammetry that detects the blood sample vitamin content.
Background technology
Vitamin (vitamin) be body be keep normal physiological function must be by a class micro-content organism of food intake, regulating metabolism and keeping aspect such as physiological function and bringing into play important effect.Long-term lacking or certain vitamin of excessive absorption all can cause corresponding disease.As yctalopia, scheroma and dry skin can appear in the A that is deficient in vitamin; The D that is deficient in vitamin can suffer from rickets; The B12 that is deficient in vitamin can suffer from pernicious anaemia; The vitamin E of excess intake can destroy coagulation function, increases hemorrhage possibility; Taking in the vitamin C that surpasses 1000 milligrams can influence the functions of expelling toxin of kidney.
At present, instrument and the detection method of human body vitamin content are had nothing in common with each other, and mainly contain microbial method, ultraviolet spectrophotometry, fluorometry, high performance liquid chromatography etc.Loaded down with trivial details consuming time, the more organic solvent of needs of pretreatment technology in the high performance liquid chromatography, and the serum requirement is bigger.The vitamin kind that ultraviolet spectrophotometry, fluorometry can detect is less.The instrument that is applied to the vitamin detection at present has: Korea S Younglin company has developed vitameter, utilizes the various vitamins in efficient liquid phase process detection food, the medicine; Germany visits a R-Biopharm company and utilizes fluorescence method to produce the instrument that detects vitamin.Because instruments such as high performance liquid chromatograph, fluorescence analyser are expensive, and need the technical skill personnel to operate, detection method is numerous and diverse, and detection time is longer, so be difficult for promoting.
Summary of the invention
The present invention provides a kind of linear sweep voltammetry that detects the blood sample vitamin content for solving the technical matters that exists in the known technology.
The purpose of this invention is to provide the vitamin electrochemical analysis method in a kind of blood sample, have highly sensitive, characteristics such as accuracy good, easy and simple to handle, quick, applied range, be suitable for medical department and use, can carry out the linear sweep voltammetry of the detection blood sample vitamin content of characteristics such as analyzing and testing to the vitamin A in the blood sample, B1, B2, B6, B9, B12, C, D, E, K1, K3.
Implementation procedure of the present invention: get a certain amount of blood sample and mix with a certain amount of homemade vitamin sample treating fluid, utilize linear sweep voltammetry to make the vitamin in the blood sample at homemade sensor probe oxidation (reduction) reaction take place, produce current signal.By comparing the current signal value of variable concentrations vitamin standard model, formulate typical curve, according to the current signal value that testing sample produces, obtain the content of vitamin in the testing sample.
The linear sweep voltammetry that the present invention detects the blood sample vitamin content for the technical scheme that solves the technical matters that exists in the known technology and take is:
A kind of linear sweep voltammetry that detects the blood sample vitamin content, be characterized in: get blood sample to be measured and mix with vitamin sample treating fluid, the current signal that utilizes the linear sweep voltammetry test sample when redox reaction takes place vitamin detector sensor probe electrode, to produce; By comparing the current signal value of variable concentrations vitamin standard model, the typical curve of formulation according to the current signal value that testing sample produces, obtains the content of vitamin in the blood sample to be measured.
The present invention detects the linear sweep voltammetry of blood sample vitamin content can also take following technical scheme:
The linear sweep voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin A content, the initial potential of detector is-1000～800mV, the termination current potential is 1000～1800mV, sweep velocity is 20～150mV/s, the sample interval is 4～20mV, and be 10～60s rest time; Range (sensitivity) is 1～500 μ A.The vitamin A sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is that one or more are formed in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, and the addition of perchlorate is 0.05～10mol/L; Sodium perchlorate, potassium perchlorate, lithium perchlorate, ammonia perchlorate be perchlorate most preferably.Strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～5mol/L; Nitric acid, perchloric acid be strong acid most preferably.The addition of dimethyl formamide is 1～100 mmol/L.
The linear sweep voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin B1 content, the initial potential of detector is-1000～800mV, the termination current potential is 1000～1800mV, sweep velocity is 20～150mV/s, the sample interval is 4～20mV, and be 10～60s rest time; Range (sensitivity) is 1～500 μ A.The vitamin B1 sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is that one or more are formed in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, and the addition of perchlorate is 0.1～10mol/L; Strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～8mol/L; The addition of dimethyl formamide is 0.01～2mmol/L.
The linear sweep voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin B2 content, the initial potential of detector is-1500～500mV, the termination current potential is-400～900mV, sweep velocity is 20～150mV/s, the sample interval is 4～20mV, and be 10～60s rest time; Range (sensitivity) is 1～500 μ A.The vitamin B2 sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is that one or more are formed in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, and the addition of perchlorate is 0.05～10mol/L; Strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～2mol/L; The addition of dimethyl formamide is 0.01～10mmol/L.
The linear sweep voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin B6 content, the initial potential of detector is-600～400mV, the termination current potential is 500～1000mV, sweep velocity is 20～150mV/s, the sample interval is 4～20mV, and be 10～60s rest time; Range (sensitivity) is 1～500 μ A.The vitamin B6 sample treatment solution is made up of phosphate buffered solution, alkaline solution, surfactant; Phosphate buffered solution is by sodium hydrogen phosphate, sodium dihydrogen phosphate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, phosphoric acid one hydrogen iron, primary iron phosphate, diammonium hydrogen phosphate, ammonium dihydrogen phosphate (ADP), one or more compositions in the calcium dihydrogen phosphate, the phosphate buffered solution addition is 0.005～10mol/L, alkaline solution is by NaOH, potassium hydroxide, calcium hydroxide, one or more compositions in the ammoniacal liquor, the alkaline solution addition is 0.01～1mol/L, surfactant is by stearic acid, neopelex, cetyl trimethyl ammonium bromide, glycerin monostearate, the fatty acid sorb is smooth, one or more compositions of polysorbate, the surfactant addition is 0.05～10mmol/L, and the fatty acid sorb is smooth, polysorbate, glycerin monostearate be surfactant most preferably.
The linear sweep voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample Cobastab 9 content, the initial potential of detector is-2000～-500mV, the termination current potential is-400～1000mV, sweep velocity is 20～150mV/s, the sample interval is 4～20mV, and be 10～60s rest time; Range (sensitivity) is 1～500 μ A.Cobastab 9 sample treatment solutions are made up of tartrate, acetate buffer solution, surfactant, ionic strength adjustor, strong acid and dimethylglyoxime ethanolic solution; Tartrate is by sodium potassium tartrate tetrahydrate, potassium antimony tartrate, tartrate ammonia potassium, sodium tartrate, one or more compositions in the potassium tartrate, potassium tartrate, sodium potassium tartrate tetrahydrate be tartrate most preferably, the tartrate addition is 2～10mmol/L, acetate buffer solution is by sodium acetate, potassium acetate, one or more are formed with acetic acid in the ammonium acetate, the acetate buffer solution addition is 0.01～10mol/L, surfactant is by stearic acid, neopelex, cetyl trimethyl ammonium bromide, glycerin monostearate, the fatty acid sorb is smooth, one or more compositions in the polysorbate, cetyl trimethyl ammonium bromide, glycerin monostearate be surfactant most preferably, the surfactant addition is 0.05～50mmol/L, ionic strength adjustor is by potassium chloride, sodium chloride, sodium sulphate, potassium chloride, one or more compositions in the potassium nitrate, the ionic strength adjustor addition is 0.01～10mol/L, strong acid is by hydrochloric acid, sulfuric acid, nitric acid, one or more compositions in the perchloric acid, sodium chloride, potassium chloride, potassium nitrate be ionic strength adjustor most preferably, the strong acid addition is 0.01～5mol/L, and the addition of dimethylglyoxime is 0.01～10mmol/L.
The linear sweep voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample cobalamin content, the initial potential of detector is-2000～-500mV, the termination current potential is-400～1000mV, sweep velocity is 20～150mV/s, the sample interval is 4～20mV, and be 10～60s rest time; Range (sensitivity) is 1～500 μ A.The cobalamin sample treatment solution is made up of tartrate, acetate buffer solution, surfactant, ionic strength adjustor, strong acid, ethanol and dimethylglyoxime; Tartrate is by sodium potassium tartrate tetrahydrate, potassium antimony tartrate, tartrate ammonia potassium, sodium tartrate, one or more compositions in the potassium tartrate, the tartrate addition is 0.02～5mol/L, acetate buffer solution is by by sodium acetate, potassium acetate, one or more are formed with acetic acid in the ammonium acetate, the acetate buffer solution addition is 0.01～10mol/L, surfactant is by stearic acid, neopelex, cetyl trimethyl ammonium bromide, glycerin monostearate, the fatty acid sorb is smooth, one or more compositions in the polysorbate, the surfactant addition is 0.02～10mmol/L, ionic strength adjustor is by potassium chloride, sodium chloride, sodium sulphate, potassium chloride, one or more compositions in the potassium nitrate, the ionic strength adjustor addition is 0.05～10mol/L, strong acid is by hydrochloric acid, sulfuric acid, nitric acid, one or more compositions in the perchloric acid, the strong acid addition is 0.01～5mol/L, and the addition of dimethylglyoxime is 0.02～10mmol/L.
The linear sweep voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample Vitamin C content, the initial potential of detector is-1000～800mV, the termination current potential is 1000～1800mV, sweep velocity is 20～150mV/s, the sample interval is 4～20mV, and be 10～60s rest time; Range (sensitivity) is 1～500 μ A.The vitamin C sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is one or more compositions in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, the perchlorate addition is 0.01～10 mol/L, strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～10mol/L; The addition of dimethyl formamide is 0.01～10mmol/L.
The linear sweep voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin D content, the initial potential of detector is-1000～800mV, the termination current potential is 1000～1800mV, sweep velocity is 20～150mV/s, the sample interval is 4～20mV, and be 10～60s rest time; Range (sensitivity) is 1～500 μ A.The vitamin D sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is one or more compositions in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, the perchlorate addition is 0.04～8 mol/L, strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.02～10mol/L; The addition of dimethyl formamide is 0.05～10mmol/L;
The linear sweep voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample content of vitamin E, the initial potential of detector is-1000～800mV, the termination current potential is 1000～1800mV, sweep velocity is 20～150mV/s, the sample interval is 4～20mV, and be 10～60s rest time; Range (sensitivity) is 1～500 μ A.The vitamin E sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is one or more compositions in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, the perchlorate addition is 0.04～8 mol/L, strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.05～10mol/L; The addition of dimethyl formamide is 0.01～2mmol/L.
The linear sweep voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin K1 content, the initial potential of detector is-1000～100mV, the termination current potential is-100～1500mV, sweep velocity is 20～150mV/s, the sample interval is 4～20mV, and be 10～60s rest time; Range (sensitivity) is 1～500 μ A.The vitamin K1 sample treatment solution is made up of in ammonium chloride, ammonium nitrate, ammoniacal liquor, hydrochloric acid, tetramethyl ammonium chloride, diethylamine, the triethylamine one or more, ammonium chloride, hydrochloric acid, tetramethyl ammonium chloride be the vitamin K1 sample treatment solution most preferably, addition is 0.02～15mol/L;
The linear sweep voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample prokeyvit content, the initial potential of detector is-1000～100mV, the termination current potential is-100～1500mV, sweep velocity is 20～150mV/s, the sample interval is 4～20mV, and be 10～60s rest time; Range (sensitivity) is 1～500 μ A.The prokeyvit sample treatment solution is made up of in ammonium chloride, ammonium nitrate, ammoniacal liquor, hydrochloric acid, tetramethyl ammonium chloride, diethylamine, the triethylamine one or more, ammonium chloride, hydrochloric acid, tetramethyl ammonium chloride be the prokeyvit sample treatment solution most preferably, addition is 0.01～5mol/L.
Advantage and good effect that the present invention has are:
Detect the linear sweep voltammetry of blood sample vitamin content owing to adopted brand-new technology scheme of the present invention, compared with prior art, that the present invention has is highly sensitive, accuracy good, easy and simple to handle, quick, applied range, be suitable for medical department and use, can carry out advantages such as analyzing and testing to the vitamin A in the blood sample, B1, B2, B6, B9, B12, C, D, E, K1, K3.
A kind of method that detects vitamin content in the blood sample provided by the invention has short, advantage such as accuracy is high, sensing range is wide of test duration, can be used for the fast detecting of blood sample vitamin content.
Description of drawings
Fig. 1 is vitamin C standard model current value of the present invention-concentration standard curve synoptic diagram.
Embodiment
For further understanding technology contents of the present invention, characteristics and effect, exemplify following examples now, and conjunction with figs. is described in detail as follows:
Consult accompanying drawing 1.
Embodiment 1
Detect the linear sweep voltammetry of blood sample vitamin content: get a certain amount of testing sample and mix with a certain amount of homemade vitamin sample treating fluid, utilize linear sweep voltammetry to make the vitamin in the testing sample at homemade sensor probe oxidation (reduction) reaction take place, produce current signal.By comparing the current signal value of variable concentrations vitamin standard model, formulate typical curve, according to the current signal value that testing sample produces, obtain the content of vitamin in the testing sample.
Detect the specific implementation process of Vitamin C content in the blood sample:
1. prepare the vitamin C sample treatment solution: the vitamin C sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is that sodium perchlorate, potassium perchlorate are formed, and the perchlorate addition is 5 mol/L, and strong acid is hydrochloric acid, sulfuric acid, and the addition of strong acid is 5mol/L; The addition of dimethyl formamide is 5mmol/L.
2. in the detection blood sample during Vitamin C content, the initial potential of detector is 200mV, and the termination current potential is 1500mV, and sweep velocity is 80mV/s, and the sample interval is 10mV, and be 30s rest time; Range (sensitivity) is 1～500 μ A
3, vitamin C standard model-concentration standard curve
(1) accurately pipettes 2000uL vitamin C sample treatment solution with pipettor;
(2) accurately pipette 5uL, 20uL, 40uL, 60uL, 100uL vitamin C standard model respectively to the vitamin C sample treatment solution with pipettor, mix;
(3) work for the treatment of electrode and auxiliary electrode under the condition of the vitamin C detected parameters that has configured, detect the record current signal value respectively;
(4) draw vitamin C standard model current value-concentration standard curve (accompanying drawing).
4, ascorbic method for quick in the blood sample
(1) accurately pipettes 2000uL vitamin C sample treatment solution with pipettor;
(2) accurately pipette the 40uL blood sample to the vitamin C sample treatment solution with pipettor, mix;
(3 work for the treatment of electrode and auxiliary electrodes under the condition of the vitamin C detected parameters that has configured, detect the record current signal value;
(4) compare with typical curve, obtain ascorbic content in the blood sample.
Claims (1)
1. linear sweep voltammetry that detects the blood sample vitamin content, it is characterized in that: get blood sample to be measured and mix with vitamin sample treating fluid, the current signal that utilizes the linear sweep voltammetry test sample when redox reaction takes place vitamin detector sensor probe, to produce; By comparing the current signal value of variable concentrations vitamin standard model, formulate typical curve, according to the current signal value that testing sample produces, obtain the content of vitamin in the blood sample to be measured;
When detecting in the blood sample Vitamin C content, the initial potential of detector is-1000～800mV, and the termination current potential is 1000～1800mV, and sweep velocity is 20～150mV/s, and the sample interval is 4～20mV, and be 10～60s rest time; The vitamin C sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is one or more compositions in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, the perchlorate addition is 0.01～10 mol/L, strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～10mol/L; The addition of dimethyl formamide is 0.01～10mmol/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110070640 CN102221574B (en) | 2011-03-23 | 2011-03-23 | Linear sweep voltammetry for detecting vitamin content of blood sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110070640 CN102221574B (en) | 2011-03-23 | 2011-03-23 | Linear sweep voltammetry for detecting vitamin content of blood sample |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102221574A CN102221574A (en) | 2011-10-19 |
| CN102221574B true CN102221574B (en) | 2013-07-03 |
Family
ID=44778183
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110070640 Expired - Fee Related CN102221574B (en) | 2011-03-23 | 2011-03-23 | Linear sweep voltammetry for detecting vitamin content of blood sample |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102221574B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106568817B (en) * | 2016-10-12 | 2019-03-05 | 衡阳师范学院 | It is a kind of for the chemically modified electrode of quantitative detection of folic acid and the preparation method of electrochemical sensor |
| CN109307720B (en) * | 2018-10-19 | 2021-08-10 | 深圳天辰医疗科技有限公司 | Composition, composite calibrator and application of composite calibrator |
| CN110646495A (en) * | 2019-09-30 | 2020-01-03 | 合肥天一生物技术研究所有限责任公司 | Convolution current voltammetry for detecting vitamin content in blood sample |
| CN112557471B (en) * | 2020-12-22 | 2023-07-18 | 合肥天一生物技术研究所有限责任公司 | Preparation method and detection method of modified electrode capable of simultaneously detecting vitamins B6 and B9 |
| CN112557468A (en) * | 2020-12-22 | 2021-03-26 | 合肥天一生物技术研究所有限责任公司 | Preparation method of modified electrode for simultaneously detecting vitamins B2 and C and electrochemical detection method |
| CN112557469A (en) * | 2020-12-22 | 2021-03-26 | 合肥天一生物技术研究所有限责任公司 | Convolution current voltammetry for simultaneously detecting vitamin A, D, E |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101320015A (en) * | 2008-07-21 | 2008-12-10 | 中国农业大学 | Method for fast detecting vitamin E content |
-
2011
- 2011-03-23 CN CN 201110070640 patent/CN102221574B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101320015A (en) * | 2008-07-21 | 2008-12-10 | 中国农业大学 | Method for fast detecting vitamin E content |
Non-Patent Citations (4)
| Title |
|---|
| 刘迎红，郝海玲，刘快之.维生素A的微分脉冲伏安法测定.《河南大学学报（自然科学版）》.2000,第30卷(第2期), * |
| 张杰,陈蓉娜,张颖衍.碳纤维电极循环伏安法检测维生素B6的条件选择.《齐齐哈尔医学院学报》.2000,第21卷(第1期), * |
| 杜小鹃，崔凤侠，韦立红等.线性扫描伏安法测定维生素C.《承德医学院学报》.2001,第18卷(第3期), * |
| 毕淑云,张敬东,刘英红,王桂芬.溶出伏安法对维生素K3的研究与测定.《理化检测-化学分册》.2006,第42卷(第6期), * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102221574A (en) | 2011-10-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102221574B (en) | Linear sweep voltammetry for detecting vitamin content of blood sample | |
| CN102221575A (en) | Differential pulse stripping voltammetry for detecting vitamin content in blood sample | |
| CN102192944B (en) | Linear scanning stripping voltammetry method for detecting content of vitamins in blood sample | |
| CN102192932B (en) | Normal pulse stripping method for detecting content of vitamin in blood samples | |
| CN104502432A (en) | Sensitive membrane, screen-printed electrode containing sensitive membrane and application thereof | |
| CN110646495A (en) | Convolution current voltammetry for detecting vitamin content in blood sample | |
| CN106442515A (en) | Simple and low-cost silver ion visual quantitative detection method | |
| CN104597090A (en) | Enzyme-free potentiometric glucose sensor and detection method thereof | |
| CN110018210A (en) | A method of directly quickly detecting uric acid | |
| Gross et al. | Nitrite/nitrate detection in serum based on dual-plate generator–collector currents in a microtrench | |
| CN104596959A (en) | Method for detecting potassium ion concentration based on DNA enzymes | |
| CN104155289A (en) | Solid electrochemical luminescence sensor for detecting mercury ions and preparation method and application of solid electrochemical luminescence sensor for detecting mercury ions | |
| CN103267783B (en) | A kind of method being detected hydrogen oxide, uric acid or uriKoxidase | |
| Song et al. | Chemiluminescence assay for uric acid in human serum and urine using flow-injection with immobilized reagents technology | |
| CN102207484B (en) | Cyclic square wave voltammetry for detecting vitamin content in blood sample | |
| CN102200527A (en) | Cyclic voltammetry for detecting vitamin content in blood sample | |
| CN102192946B (en) | Square wave volt-ampere method for detecting vitamin content in blood samples | |
| CN105784699A (en) | Potassium ion reagent with better stability and higher sensitivity and detection method | |
| CN103115882B (en) | Method for detecting H2O2 | |
| CN105301261A (en) | Kit for detecting HbAlc (Glycosylated Hemoglobin), and preparation method and using method of kit | |
| CN102192945A (en) | Osteryoung square wave stripping voltammetry for detecting content of vitamins in blood sample | |
| CN206248606U (en) | A kind of measuring electrode for detecting uric acid | |
| CN104458728A (en) | Method for detecting concentration of sodium ions | |
| CN101620069A (en) | Manganese ion diagnosis/determination kit and method for determining manganese ion concentration | |
| CN101625312A (en) | Manganese ion diagnostic/assay kit and method for assaying concentration of manganese ions |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130703 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |