CN102221575A - Differential pulse stripping voltammetry for detecting vitamin content in blood sample - Google Patents
Differential pulse stripping voltammetry for detecting vitamin content in blood sample Download PDFInfo
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Abstract
The invention which relates to differential pulse stripping voltammetry for detecting a vitamin content in a blood sample belongs to the technical field of vitamin analysis and detection. Differential pulse stripping voltammetry is characterized in that: the blood sample to be detected and a vitamin sample treating liquid are mixed, and a current signal which is genearated when the blood sample is subjected to an oxidation reduction reaction on a sensor probe of a vitamin detector through differential pulse stripping voltammetry; and establishing a standard curve through comparing current signal values of vitamin standard samples with different concentrations and acquiring the vitamin content in the blood sample to be detected according to the current signal value of the blood sample to be detected. Differential pulse stripping voltammetry has the advantages of high sensitivity, good accuracy, simple and fast operation and wide application scope, is suitable for health care departments to use, allows vitamins, A, B1, B2, B6, B9, B12, C, D, E, K1 and K3, in the blood sample to be analyzed and detected, so differential pulse stripping voltammetry can be used to rapidly detect vitamin contents in the blood sample.
Description
Technical field
The invention belongs to the vitamin technical field of analysis and detection, particularly relate to a kind of differential pulse stripping voltammetry that detects the blood sample vitamin content.
Background technology
Vitamin (vitamin) be body be keep normal physiological function must be by a class micro-content organism of food intake, regulating metabolism and keeping aspect such as physiological function and bringing into play important effect.Long-term lacking or certain vitamin of excessive absorption all can cause corresponding disease.As yctalopia, scheroma and dry skin can appear in the A that is deficient in vitamin; The D that is deficient in vitamin can suffer from rickets; The B12 that is deficient in vitamin can suffer from pernicious anaemia; The vitamin E of excess intake can destroy coagulation function, increases hemorrhage possibility; Taking in the vitamin C that surpasses 1000 milligrams can influence the functions of expelling toxin of kidney.
At present, the instrument and the detection method of human body vitamin content are had nothing in common with each other, and mainly contain microbial method, ultraviolet spectrophotometry, fluorometry, high performance liquid chromatography etc.Loaded down with trivial details consuming time, the more organic solvent of needs of pretreatment technology in the high performance liquid chromatography, and the serum requirement is bigger.The vitamin kind that ultraviolet spectrophotometry, fluorometry can detect is less.The instrument that is applied to the vitamin detection at present has: Korea S Younglin company has developed vitameter, utilizes the various vitamins in high performance liquid chromatogram method detection food, the medicine; Germany visits a R-Biopharm company and utilizes fluorescence method to produce the instrument that detects vitamin.Because instruments such as high performance liquid chromatograph, fluorescence analyser cost an arm and a leg, and need the technical skill personnel to operate, detection method is numerous and diverse, and detection time is longer, so be difficult for promoting.
Summary of the invention
The present invention provides a kind of differential pulse stripping voltammetry that detects the blood sample vitamin content for solving the technical matters that exists in the known technology.
The purpose of this invention is to provide the vitamin electrochemical analysis method in a kind of blood sample, have highly sensitive, characteristics such as accuracy good, easy and simple to handle, quick, applied range, be suitable for medical department and use, can carry out the differential pulse stripping voltammetry of the detection blood sample vitamin content of characteristics such as analyzing and testing the vitamin A in the blood sample, B1, B2, B6, B9, B12, C, D, E, K1, K3.
Implementation procedure of the present invention: get a certain amount of blood sample and mix, utilize the differential pulse stripping voltammetry to make the vitamin in the blood sample that oxidation (reduction) reaction take place on homemade sensor probe, produce current signal with a certain amount of homemade vitamin sample treating fluid.By comparing the current signal value of variable concentrations vitamin standard model, formulate typical curve, according to the current signal value that testing sample produces, obtain the content of vitamin in the testing sample.
The differential pulse stripping voltammetry that the present invention detects the blood sample vitamin content for the technical scheme that solves the technical matters that exists in the known technology and take is:
A kind of differential pulse stripping voltammetry that detects the blood sample vitamin content, be characterized in: get blood sample to be measured and mix, the current signal that produces when utilizing differential pulse stripping voltammetry test sample on vitamin detector sensor probe electrode, redox reaction to take place with vitamin sample treating fluid; By comparing the current signal value of variable concentrations vitamin standard model, the typical curve of formulation according to the current signal value that testing sample produces, obtains the content of vitamin in the blood sample to be measured.
The present invention detects the differential pulse stripping voltammetry of blood sample vitamin content can also take following technical scheme:
The differential pulse stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin A content, the enrichment electro-deposition current potential of detector is-200～400mV, the enrichment electrodeposition time is 10～90s, initial potential is-200～400mV, the termination current potential is 1000～1800mV, the current potential increment is 2～15mV, pulse height is 10～60mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 1～500 μ A.The vitamin A sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is that one or more are formed in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, and the addition of perchlorate is 0.05～10mol/L; Sodium perchlorate, potassium perchlorate, lithium perchlorate, ammonia perchlorate be perchlorate most preferably.Strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～5mol/L; Nitric acid, perchloric acid be strong acid most preferably.The addition of dimethyl formamide is 1～100 mmol/L.
The differential pulse stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin B1 content, the enrichment electro-deposition current potential of detector is-400～400mV, the enrichment electrodeposition time is 10～60s, initial potential is-200～400mV, the termination current potential is 1000～1800mV, the current potential increment is 5～20mV, pulse height is 10～60mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 1～500 μ A.The vitamin B1 sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is that one or more are formed in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, and the addition of perchlorate is 0.1～10mol/L; Strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～8mol/L; The addition of dimethyl formamide is 0.01～2mmol/L.
The differential pulse stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin B2 content, the enrichment electro-deposition current potential of detector is-1500～-500mV, the enrichment electrodeposition time is 20～80s, initial potential is-1500～-500mV, the termination current potential is-400～900mV, the current potential increment is 2～15mV, pulse height is 10～80mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 1～500 μ A.The vitamin B2 sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is that one or more are formed in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, and the addition of perchlorate is 0.05～10mol/L; Strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～2mol/L; The addition of dimethyl formamide is 0.01～10mmol/L.
The differential pulse stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin B6 content, the enrichment electro-deposition current potential of detector is-600～400mV, the enrichment electrodeposition time is 10～60s, initial potential is-600～400mV, the termination current potential is 500～1000mV, the current potential increment is 2～15mV, pulse height is 20～80mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 1～500 μ A.The vitamin B6 sample treatment solution is made up of phosphate buffered solution, alkaline solution, surfactant; Phosphate buffered solution is by sodium hydrogen phosphate, sodium dihydrogen phosphate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, phosphoric acid one hydrogen iron, primary iron phosphate, diammonium hydrogen phosphate, ammonium dihydrogen phosphate (ADP), one or more compositions in the calcium dihydrogen phosphate, the phosphate buffered solution addition is 0.005～10mol/L, alkaline solution is by NaOH, potassium hydroxide, calcium hydroxide, one or more compositions in the ammoniacal liquor, the alkaline solution addition is 0.01～1mol/L, surfactant is by stearic acid, neopelex, cetyl trimethyl ammonium bromide, glycerin monostearate, the fatty acid sorb is smooth, one or more compositions of polysorbate, the fatty acid sorb is smooth, polysorbate, glycerin monostearate be surfactant most preferably, the surfactant addition is 0.05～10mmol/L.
The differential pulse stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample Cobastab 9 content, the enrichment electro-deposition current potential of detector is-2000～-500mV, the enrichment electrodeposition time is 20～80s, initial potential is-2000～-1000mV, the termination current potential is-400～1000mV, the current potential increment is 2～15mV, pulse height is 10～80mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 1 ~ 500 μ A.Cobastab 9 sample treatment solutions are made up of tartrate, acetate buffer solution, surfactant, ionic strength adjustor, strong acid and dimethylglyoxime ethanolic solution; Tartrate is by sodium potassium tartrate tetrahydrate, potassium antimony tartrate, tartrate ammonia potassium, sodium tartrate, one or more compositions in the potassium tartrate, potassium tartrate, sodium potassium tartrate tetrahydrate be tartrate most preferably, the tartrate addition is 2～10mmol/L, acetate buffer solution is by sodium acetate, potassium acetate, one or more are formed with acetic acid in the ammonium acetate, the acetate buffer solution addition is 0.01～10mol/L, surfactant is by stearic acid, neopelex, cetyl trimethyl ammonium bromide, glycerin monostearate, the fatty acid sorb is smooth, one or more compositions in the polysorbate, cetyl trimethyl ammonium bromide, glycerin monostearate be surfactant most preferably, the surfactant addition is 0.05～50mmol/L, ionic strength adjustor is by potassium chloride, sodium chloride, sodium sulphate, potassium chloride, one or more compositions in the potassium nitrate, the ionic strength adjustor addition is 0.01～10mol/L, strong acid is by hydrochloric acid, sulfuric acid, nitric acid, one or more compositions in the perchloric acid, sodium chloride, potassium chloride, potassium nitrate be ionic strength adjustor most preferably, the strong acid addition is 0.01～5mol/L, and the addition of dimethylglyoxime is 0.01～10mmol/L.
The differential pulse stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample cobalamin content, the enrichment electro-deposition current potential of detector is-2000～-500mV, enrichment (electro-deposition) time is 20～80s, initial potential is-2000～-1000mV, the termination current potential is-400～1000mV, the current potential increment is 2～15mV, pulse height is 10～80mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 1 ~ 500 μ A.The cobalamin sample treatment solution is made up of tartrate, acetate buffer solution, surfactant, ionic strength adjustor, strong acid, ethanol and dimethylglyoxime; Tartrate is by sodium potassium tartrate tetrahydrate, potassium antimony tartrate, tartrate ammonia potassium, sodium tartrate, one or more compositions in the potassium tartrate, the tartrate addition is 0.02～5mol/L, acetate buffer solution is by by sodium acetate, potassium acetate, one or more are formed with acetic acid in the ammonium acetate, the acetate buffer solution addition is 0.01～10mol/L, surfactant is by stearic acid, neopelex, cetyl trimethyl ammonium bromide, glycerin monostearate, the fatty acid sorb is smooth, one or more compositions in the polysorbate, the surfactant addition is 0.02～10mmol/L, ionic strength adjustor is by potassium chloride, sodium chloride, sodium sulphate, potassium chloride, one or more compositions in the potassium nitrate, the ionic strength adjustor addition is 0.05～10mol/L, strong acid is by hydrochloric acid, sulfuric acid, nitric acid, one or more compositions in the perchloric acid, the strong acid addition is 0.01～5mol/L, and the addition of dimethylglyoxime is 0.02～10mmol/L.
The differential pulse stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample Vit C contents, the enrichment electro-deposition current potential of detector is-400～400mV, the enrichment electrodeposition time is 10～60s, initial potential is-200～400mV, the termination current potential is 1000～1800mV, the current potential increment is 5～20mV, pulse height is 10～60mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 1 ~ 500 μ A.The vitamin C sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is one or more compositions in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, the perchlorate addition is 0.01～10 mol/L, strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～10mol/L; The addition of dimethyl formamide is 0.01～10mmol/L.
The differential pulse stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin D content, the enrichment electro-deposition current potential of detector is-200～400mV, the enrichment electrodeposition time is 10～90s, initial potential is-200～400mV, the termination current potential is 1000～1800mV, the current potential increment is 2～15mV, pulse height is 10～60mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 1 ~ 500 μ A.The vitamin D sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is one or more compositions in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, the perchlorate addition is 0.04～8 mol/L, strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.02～10mol/L; The addition of dimethyl formamide is 0.05～10mmol/L;
The differential pulse stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample content of vitamin E, the enrichment electro-deposition current potential of detector is-200～400mV, the enrichment electrodeposition time is 10～90s, initial potential is-200～400mV, the termination current potential is 1000～1800mV, the current potential increment is 2～15mV, pulse height is 10～60mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 1 ~ 500 μ A.The vitamin E sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is one or more compositions in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, the perchlorate addition is 0.04～8 mol/L, strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.05～10mol/L; The addition of dimethyl formamide is 0.01～2mmol/L.
The differential pulse stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin K1 content, the enrichment electro-deposition current potential of detector is 100～1000mV, the enrichment electrodeposition time is 20～80s, initial potential is-1000～100mV, the termination current potential is-100～1500mV, the current potential increment is 2～15mV, pulse height is 10～80mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 1 ~ 500 μ A.The vitamin K1 sample treatment solution is made up of in ammonium chloride, ammonium nitrate, ammoniacal liquor, hydrochloric acid, tetramethyl ammonium chloride, diethylamine, the triethylamine one or more, ammonium chloride, hydrochloric acid, tetramethyl ammonium chloride be the vitamin K1 sample treatment solution most preferably, addition is 0.02～15mol/L;
The differential pulse stripping voltammetry of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample prokeyvit content, the enrichment electro-deposition current potential of detector is 100～1000mV, the enrichment electrodeposition time is 20～80s, initial potential is-1000～100mV, the termination current potential is-100～1500mV, the current potential increment is 2～15mV, pulse height is 10～80mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; Range (sensitivity) is 1 ~ 500 μ A.The prokeyvit sample treatment solution is made up of in ammonium chloride, ammonium nitrate, ammoniacal liquor, hydrochloric acid, tetramethyl ammonium chloride, diethylamine, the triethylamine one or more, ammonium chloride, hydrochloric acid, tetramethyl ammonium chloride be the prokeyvit sample treatment solution most preferably, addition is 0.01～5mol/L.
Advantage and good effect that the present invention has are:
Detect the differential pulse stripping voltammetry of blood sample vitamin content owing to adopted brand-new technology scheme of the present invention, compared with prior art, that the present invention has is highly sensitive, accuracy good, easy and simple to handle, quick, applied range, be suitable for medical department and use, can carry out advantages such as analyzing and testing the vitamin A in the blood sample, B1, B2, B6, B9, B12, C, D, E, K1, K3.
A kind of method that detects vitamin content in the blood sample provided by the invention has short, advantage such as accuracy is high, sensing range is wide of test duration, can be used for the fast detecting of blood sample vitamin content.
Description of drawings
Fig. 1 is vitamin A standard model current value of the present invention-concentration standard curve synoptic diagram.
Embodiment
For further understanding technology contents of the present invention, characteristics and effect, exemplify following examples now, and conjunction with figs. is described in detail as follows:
Consult accompanying drawing 1.
Embodiment 1
Detect the differential pulse stripping voltammetry of blood sample vitamin content: get a certain amount of testing sample and mix with a certain amount of homemade vitamin sample treating fluid, utilize the differential pulse stripping voltammetry to make the vitamin in the testing sample that oxidation (reduction) reaction take place on homemade sensor probe, produce current signal.By comparing the current signal value of variable concentrations vitamin standard model, formulate typical curve, according to the current signal value that testing sample produces, obtain the content of vitamin in the testing sample.
Detect the specific implementation process of vitamin A content in the blood sample:
1. prepare the vitamin A sample treatment solution: vitamin A sample treatment solution perchlorate, strong acid and dimethyl formamide are formed; Perchlorate is sodium perchlorate, potassium perchlorate, and addition is 2mol/L; Strong acid is nitric acid, perchloric acid, and addition is 2mol/L; The addition of dimethyl formamide is 30mmol/L.
2. in the detection blood sample during vitamin A content, the enrichment electro-deposition current potential of detector is 200mV, and the enrichment electrodeposition time is 50s, initial potential is 200mV, the termination current potential is 1300mV, and the current potential increment is 10mV, and pulse height is 30mV, pulse width is 0.06s, recurrence interval is 0.2s, and the sampling width is 0.03s, and be 40s rest time, resting potential is 300mV, and the stop time is 30s; Range (sensitivity) is 1 ~ 500 μ A.
3, vitamin A standard model-concentration standard curve
(1) accurately pipettes 2000uL vitamin A sample treatment solution with pipettor;
(2) accurately pipette 8uL, 20uL, 40uL, 80uL, 160uL vitamin A standard model respectively to the vitamin A sample treatment solution with pipettor, mix;
(3) work of treatment electrode and auxiliary electrode under the condition of the vitamin A detected parameters that has configured, detect the record current signal value respectively;
(4) draw vitamin A standard model current value-concentration standard curve (accompanying drawing).
4, the method for quick of vitamin A in the blood sample
(1) accurately pipettes 2000uL vitamin A sample treatment solution with pipettor;
(2) accurately pipette the 80uL blood sample to the vitamin A sample treatment solution with pipettor, mix;
(3 work of treatment electrode and auxiliary electrodes under the condition of the vitamin A detected parameters that has configured, detect the record current signal value;
(4) compare with typical curve, obtain the content of vitamin A in the blood sample.
Claims (10)
1. differential pulse stripping voltammetry that detects the blood sample vitamin content, it is characterized in that: get blood sample to be measured and mix, the current signal that produces when utilizing differential pulse stripping voltammetry test sample on vitamin detector sensor probe, redox reaction to take place with vitamin sample treating fluid; By comparing the current signal value of variable concentrations vitamin standard model, formulate typical curve, according to the current signal value that testing sample produces, obtain the content of vitamin in the blood sample to be measured.
2. according to the differential pulse stripping voltammetry of the described detection blood sample of claim 1 vitamin content, it is characterized in that: when detecting in the blood sample vitamin A content, the enrichment electro-deposition current potential of detector is-200～400mV, the enrichment electrodeposition time is 10～90s, initial potential is-200～400mV, the termination current potential is 1000～1800mV, the current potential increment is 2～15mV, pulse height is 10～60mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; The vitamin A sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is that one or more are formed in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, and the addition of perchlorate is 0.05～10mol/L; Strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～5mol/L; The addition of dimethyl formamide is 1～100 mmol/L.
3. according to the differential pulse stripping voltammetry of the described detection blood sample of claim 1 vitamin content, it is characterized in that: when detecting in the blood sample vitamin B1 content, the enrichment electro-deposition current potential of detector is-400～400mV, the enrichment electrodeposition time is 10～60s, initial potential is-200～400mV, the termination current potential is 1000～1800mV, the current potential increment is 5～20mV, pulse height is 10～60mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; The vitamin B1 sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is that one or more are formed in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, and the addition of perchlorate is 0.1～10mol/L; Strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～8mol/L; The addition of dimethyl formamide is 0.01～2mmol/L.
4. in the detection blood sample during vitamin B2 content, the enrichment electro-deposition current potential of detector is-1500～-500mV, the enrichment electrodeposition time is 20～80s, initial potential is-1500～-500mV, the termination current potential is-400～900mV, and the current potential increment is 2～15mV, and pulse height is 10～80mV, pulse width is 0.04～0.08s, recurrence interval is 0.1～0.3s, and the sampling width is 0.01～0.05s, and be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s; The vitamin B2 sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is that one or more are formed in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, and the addition of perchlorate is 0.05～10mol/L; Strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～2mol/L; The addition of dimethyl formamide is 0.01～10mmol/L.
5. according to the differential pulse stripping voltammetry of the described detection blood sample of claim 1 vitamin content, it is characterized in that: when detecting in the blood sample vitamin B6 content, the enrichment electro-deposition current potential of detector is-600～400mV, the enrichment electrodeposition time is 10～60s, initial potential is-600～400mV, the termination current potential is 500～1000mV, the current potential increment is 2～15mV, pulse height is 20～80mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; The vitamin B6 sample treatment solution is made up of phosphate buffered solution, alkaline solution, surfactant; Phosphate buffered solution is by sodium hydrogen phosphate, sodium dihydrogen phosphate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, phosphoric acid one hydrogen iron, primary iron phosphate, diammonium hydrogen phosphate, ammonium dihydrogen phosphate (ADP), one or more compositions in the calcium dihydrogen phosphate, the phosphate buffered solution addition is 0.005～10mol/L, alkaline solution is by NaOH, potassium hydroxide, calcium hydroxide, one or more compositions in the ammoniacal liquor, the alkaline solution addition is 0.01～1mol/L, surfactant is by stearic acid, neopelex, cetyl trimethyl ammonium bromide, glycerin monostearate, the fatty acid sorb is smooth, one or more compositions of polysorbate, surfactant addition are 0.05～10mmol/L.
6. according to the differential pulse stripping voltammetry of the described detection blood sample of claim 1 vitamin content, it is characterized in that: when detecting in the blood sample Cobastab 9 content, the enrichment electro-deposition current potential of detector is-2000～-500mV, the enrichment electrodeposition time is 20～80s, initial potential is-2000～-1000mV, the termination current potential is-400～1000mV, the current potential increment is 2～15mV, pulse height is 10～80mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; Cobastab 9 sample treatment solutions are made up of tartrate, acetate buffer solution, surfactant, ionic strength adjustor, strong acid and dimethylglyoxime ethanolic solution; Tartrate is by sodium potassium tartrate tetrahydrate, potassium antimony tartrate, tartrate ammonia potassium, sodium tartrate, one or more compositions in the potassium tartrate, the tartrate addition is 2～10mmol/L, acetate buffer solution is by sodium acetate, potassium acetate, one or more are formed with acetic acid in the ammonium acetate, the acetate buffer solution addition is 0.01～10mol/L, surfactant is by stearic acid, neopelex, cetyl trimethyl ammonium bromide, glycerin monostearate, the fatty acid sorb is smooth, one or more compositions in the polysorbate, the surfactant addition is 0.05～50mmol/L, ionic strength adjustor is by potassium chloride, sodium chloride, sodium sulphate, potassium chloride, one or more compositions in the potassium nitrate, the ionic strength adjustor addition is 0.01～10mol/L, strong acid is by hydrochloric acid, sulfuric acid, nitric acid, one or more compositions in the perchloric acid, the strong acid addition is 0.01～5mol/L, and the addition of dimethylglyoxime is 0.01～10mmol/L.
7. according to the differential pulse stripping voltammetry of the described detection blood sample of claim 1 vitamin content, it is characterized in that: when detecting in the blood sample cobalamin content, the enrichment electro-deposition current potential of detector is-2000～-500mV, the enrichment electrodeposition time is 20～80s, initial potential is-2000～-1000mV, the termination current potential is-400～1000mV, the current potential increment is 2～15mV, pulse height is 10～80mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; The cobalamin sample treatment solution is made up of tartrate, acetate buffer solution, surfactant, ionic strength adjustor, strong acid, ethanol and dimethylglyoxime; Tartrate is by sodium potassium tartrate tetrahydrate, potassium antimony tartrate, tartrate ammonia potassium, sodium tartrate, one or more compositions in the potassium tartrate, the tartrate addition is 0.02～5mol/L, acetate buffer solution is by sodium acetate, potassium acetate, one or more are formed with acetic acid in the ammonium acetate, the acetate buffer solution addition is 0.01～10mol/L, surfactant is by stearic acid, neopelex, cetyl trimethyl ammonium bromide, glycerin monostearate, the fatty acid sorb is smooth, one or more compositions in the polysorbate, the surfactant addition is 0.02～10mmol/L, ionic strength adjustor is by potassium chloride, sodium chloride, sodium sulphate, potassium chloride, one or more compositions in the potassium nitrate, the ionic strength adjustor addition is 0.05～10mol/L, strong acid is by hydrochloric acid, sulfuric acid, nitric acid, one or more compositions in the perchloric acid, the strong acid addition is 0.01～5mol/L, and the addition of dimethylglyoxime is 0.02～10mmol/L.
8. according to the differential pulse stripping voltammetry of the described detection blood sample of claim 1 vitamin content, it is characterized in that: when detecting in the blood sample Vit C contents, the enrichment electro-deposition current potential of detector is-400～400mV, the enrichment electrodeposition time is 10～60s, initial potential is-200～400mV, the termination current potential is 1000～1800mV, the current potential increment is 5～20mV, pulse height is 10～60mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; The vitamin C sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is one or more compositions in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, the perchlorate addition is 0.01～10 mol/L, strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～10mol/L; The addition of dimethyl formamide is 0.01～10mmol/L.
9. according to the differential pulse stripping voltammetry of the described detection blood sample of claim 1 vitamin content, it is characterized in that: when detecting in the blood sample vitamin D content, the enrichment electro-deposition current potential of detector is-200～400mV, the enrichment electrodeposition time is 10～90s, initial potential is-200～400mV, the termination current potential is 1000～1800mV, the current potential increment is 2～15mV, pulse height is 10～60mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; The vitamin D sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is one or more compositions in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, the perchlorate addition is 0.04～8 mol/L, strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.02～10mol/L; The addition of dimethyl formamide is 0.05～10mmol/L;
Differential pulse stripping voltammetry according to the described detection blood sample of claim 1 vitamin content, it is characterized in that: when detecting in the blood sample content of vitamin E, the enrichment electro-deposition current potential of detector is-200～400mV, the enrichment electrodeposition time is 10～90s, initial potential is-200～400mV, the termination current potential is 1000～1800mV, the current potential increment is 2～15mV, pulse height is 10～60mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; The vitamin E sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is one or more compositions in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, the perchlorate addition is 0.04～8 mol/L, strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.05～10mol/L; The addition of dimethyl formamide is 0.01～2mmol/L.
10. according to the differential pulse stripping voltammetry of the described detection blood sample of claim 1 vitamin content, it is characterized in that: when detecting in the blood sample vitamin K1 content, the enrichment electro-deposition current potential of detector is 100～1000mV, the enrichment electrodeposition time is 20～80s, initial potential is-1000～100mV, the termination current potential is-100～1500mV, the current potential increment is 2～15mV, pulse height is 10～80mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, the sampling width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; The vitamin K1 sample treatment solution is made up of in ammonium chloride, ammonium nitrate, ammoniacal liquor, hydrochloric acid, tetramethyl ammonium chloride, diethylamine, the triethylamine one or more, and addition is 0.02～15mol/L;
When detecting in the blood sample prokeyvit content, the enrichment electro-deposition current potential of detector is 100～1000mV, and the enrichment electrodeposition time is 20～80s, initial potential is-1000～100mV, the termination current potential is-100～1500mV, and the current potential increment is 2～15mV, and pulse height is 10～80mV, pulse width is 0.04～0.08s, recurrence interval is 0.1～0.3s, and the sampling width is 0.01～0.05s, and be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s; The prokeyvit sample treatment solution is made up of in ammonium chloride, ammonium nitrate, ammoniacal liquor, hydrochloric acid, tetramethyl ammonium chloride, diethylamine, the triethylamine one or more, and addition is 0.01～5mol/L.
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